CN108260737A - A kind of production technology for the ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit - Google Patents
A kind of production technology for the ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit Download PDFInfo
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- CN108260737A CN108260737A CN201810156340.1A CN201810156340A CN108260737A CN 108260737 A CN108260737 A CN 108260737A CN 201810156340 A CN201810156340 A CN 201810156340A CN 108260737 A CN108260737 A CN 108260737A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
Abstract
The invention discloses a kind of production technologies for the ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit, specifically include：Hybrid bacterial strain prepares, Chinese medicine material prepares, fermentation prepares, raw material fermentation.By production technology disclosed by the invention, the product being prepared can enhance immunity of meat chickens, prevent the generation of disease, while improve stress ability, promote growth of meat chicken；And preparation process disclosed by the invention is simple, and ginkgo leaf with plurality of Chinese is mixed, is fermented using specific microorganism, can integrate the effect of playing each traditional Chinese medicine ingredients, enhances the compatibility effect of Chinese medicine.
Description
Technical field
The present invention relates to broiler chicken feed additive technical field, more particularly to a kind of enhancing broiler feeding benefit
Ginkgo leaf compound fermentation preparation production technology.
Background technology
Chicken be quite by the favorite meat of people, to it is malnutritive, chilly is cold, weak fatigue, irregular menstruation, anaemia,
Weakness etc. has good dietary function, is not only suitable for propagandizing hotly, stewing, and being that comparison is suitble to the cold and dressed with sauce meat of cold food.And with
The improvement of people's living standards, it is increasing to the amount of meat, promote the development of aquaculture, especially broiler chicken aquaculture.
It is easy to infect various diseases during growth of meat chicken, especially during broiler chicken large-scale farming, due to meat
The hypoimmunity of chicken once disease, especially respiratory tract class disease occurs, tends to infect, causes large batch of death；
And cause that broiler chicken appetite is low, and deliver for sale reduces again, marketing time extends, and huge loss is brought to broiler breeding.
To solve the problems, such as broiler breeding process, at present frequently with the mode of first treatment after the onset, find ill
It is just administered after chicken, therapic opportunity best at this time may have already passed by, it is difficult to make up.And best mode should be to prevent to be
It is main, promoted broiler chicken disease resistance be only solve the problems, such as it is basic.To improve the immunity of broiler chicken, for a long time lot of domestic and foreign
Researcher has done many unremitting efforts, has application process of some feed addictives on broiler chicken at present, but all there is effects
The problem of fruit is single, and effect is limited is merely capable of reaching raising immunity to a certain extent, and broiler chicken cannot be promoted to give birth to
It is long.
Therefore, it is necessary to a kind of feed addictives, can enhance immunity of meat chickens, prevent the generation of disease, while can have
The promotion growth of meat chicken of effect.
Invention content
In view of this, the present invention provides a kind of production works for the ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit
Skill, the product being prepared can enhance immunity of meat chickens, prevent the generation of disease, while improve stress ability, promote broiler chicken
Growth；And the bright disclosed preparation process of this law is simple, and ginkgo leaf with plurality of Chinese is mixed, is carried out using specific microorganism
Fermentation can integrate the effect of playing each traditional Chinese medicine ingredients, enhance the compatibility effect of Chinese medicine.
A kind of production technology for the ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit, which is characterized in that specific packet
Include following steps：
(1) hybrid bacterial strain prepares：Excellent bacterial strain is screened, tablet is first carried out and spreads cultivation, then carry out Liquid Culture, it is spare；
(2) Chinese medicine material prepares：The Chinese medicine material including ginkgo leaf is weighed, it is spare；
(3) fermentation prepares：By above-mentioned Chinese medicine material after mixing, hybrid bacterial strain is accessed, and adds brown sugar, supplements water
Point, it is packed, sealed using respiratory membrane, obtain raw material；
(4) above-mentioned raw material are subjected to fermentation process.
A kind of production technology of ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit disclosed by the invention, utilizes spy
Fixed microorganism is fermented, and can integrate the effect of playing traditional Chinese medicine ingredients, enhances the compatibility effect of Chinese medicine；And whole preparation
Method is simple, easily implements, and has certain development prospect.
Preferably, the excellent bacterial strain screened in the step (1) include aspergillus oryzae, lactobacillus thermophilus, lactobacillus plantarum and
Candida utili.
The bacterial strain that the present invention uses is to be allowed in the country feed addictive probiotics strain, can make Chinese medicine material
Rapid Fermentation, and be not easy to be killed by certain ingredients in Chinese medicine, without antagonism, the hair of energy maximal efficiency between each bacterial strain
Ferment.
Preferably, the aspergillus oryzae in the step (1), lactobacillus thermophilus, lactobacillus plantarum and candida utili are matched
Than being 2~4：1~4：1~3：1~2.
Preferably, the aspergillus oryzae in the step (1), lactobacillus thermophilus, lactobacillus plantarum and candida utili are matched
Than being 2：2：2：1.
Under conditions of mixture ratios disclosed by the invention, each bacterium growth is most fast, can obtain the maximum amount of total bacteria count, obtained mixing
Bacterial strain effect is more excellent, is more advantageous to the fermentation of Chinese medicine material, so as to be conducive to prepare compound fermentation preparation.
Preferably, in the step (1) Liquid Culture to 109~1010A/mL.
Preferably, step (2) Chinese medicine material includes the component of following parts by weight：4~7 parts of ginkgo leaves, 2~4 portions of fishes
Raw meat grass, 1~3 part of Radix Astragali, 1~2 part of radix scutellariae and 2~5 portions of selfheals
Preferably, step (2) Chinese medicine material includes the component of following parts by weight：5 parts of ginkgo leaves, 3 parts of cordate houttuynias, 1
Part Radix Astragali, 1 part of radix scutellariae and 2 portions of selfheals.
Under proportioning disclosed by the invention, the probiotics that Chinese medicine material can be added fully is decomposed, the activity of Chinese herbal medicine
Adequately discharged.It is and living containing the biology such as flavones, alkaloid, polysaccharide, saponin(e, terpene in the Chinese medicine material of the invention used
Property substance, it is safe and non-toxic, the immune efficacy of broiler chicken can be effectively improved after probiotics is decomposed, have it is antiviral, improve broiler chicken
Intestinal health promotes the effect of growth of meat chicken, and can also provide part growth of meat chicken necessary nutritional ingredient, promotes broiler chicken life
It is long.
Preferably, the mass ratio of the volume of hybrid bacterial strain and Chinese medicine material is 5~7 in the step (3)：100；Brown sugar with
The mass ratio of Chinese medicine material is 8~10：100；Water volume is 35-40 with Chinese medicine material mass ratio:100.
Under the conditions disclosed in the present invention this, the nutritional ingredient and moisture content being capable of providing needed for strain growth so that bacterial strain is fast
Fast-growing is long, and total bacteria count content is high；And so that the active ingredient in Chinese medicine material is fully decomposed, so as to make in Chinese medicine material
Activity is adequately discharged.
Preferably, 0.5~2Kg of respiratory membrane loading amount in the step (3).
Preferably, fermentation temperature is 20~35 DEG C in the step (4), 10~15d of fermentation time.
On this condition, most fast, the total bacteria count content highest of acquisition, effective component of chinese medicine are grown after each bacterium compatibility combination
Most fast decomposition can be obtained, fermenting-ripening is fastest.
It can be seen via above technical scheme that compared with prior art, the present disclosure provides a kind of enhancing broiler chicken to raise
The production technology of the ginkgo leaf compound fermentation preparation of benefit is supported, the product being prepared can enhance immunity of meat chickens, prevent disease
The generation of disease, while stress ability is improved, promote growth of meat chicken.And the bright disclosed preparation process of this law is simple, by ginkgo leaf
It mixes with plurality of Chinese, is fermented using specific microorganism, the effect of playing each traditional Chinese medicine ingredients can be integrated, enhance Chinese medicine
Compatibility effect；After fermentation so that the component contents such as flavones, fiber decline, and the nutritional ingredients such as amino acid, crude protein significantly carry
It rises, and produces certain lactic acid, alcohol, so as to increase the prebiotic bacterial content of product, palatability and nutritional ingredient.
Specific embodiment
The technical solution in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel all other embodiments obtained without making creative work belong to the model that the present invention protects
It encloses.
Embodiment 1
A kind of production technology for the ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit, specifically comprises the following steps：
(1) hybrid bacterial strain prepares：Screen excellent aspergillus oryzae, lactobacillus thermophilus, lactobacillus plantarum and candida utili
It carries out tablet to spread cultivation, then Liquid Culture is to 109A/mL；Then according to aspergillus oryzae, lactobacillus thermophilus, lactobacillus plantarum and production protein
The proportioning of Candida is 2：2：2：1 mixing for standby use；
(2) Chinese medicine material prepares：Weigh 417 grams of part ginkgo leaves, 250 grams of cordate houttuynias, 83 grams of Radix Astragalis, 83 grams of radix scutellariaes and 167
Gram selfheal, crushing sieve with 100 mesh sieve spare；
(3) fermentation prepares：By Chinese medicine material after mixing, the hybrid bacterial strain that access 50mL steps (1) obtain, Ran Houtian
Add 80g brown sugar, while supplement 350mL moisture, packed using the respiratory membrane of loading amount 0.5Kg, sealing, obtain raw material；
(4) by raw material under the conditions of 30 DEG C, ferment 15d.
Embodiment 2
A kind of production technology for the ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit, specifically comprises the following steps：
(1) hybrid bacterial strain prepares：Screen excellent aspergillus oryzae, lactobacillus thermophilus, lactobacillus plantarum and candida utili
It carries out tablet to spread cultivation, then Liquid Culture is to 1010A/mL；Then according to aspergillus oryzae, lactobacillus thermophilus, lactobacillus plantarum and production protein
The proportioning of Candida is 4：2：2：2, by bacterial strain mixing for standby use；
(2) Chinese medicine material prepares：Weigh 468g parts of ginkgo leaves, 133g cordate houttuynias, 133g Radix Astragalis, 133g radix scutellariaes and 133g summers
Withered grass, crushing sieve with 100 mesh sieve spare；
(3) fermentation prepares：By Chinese medicine material after mixing, the hybrid bacterial strain that access 70mL is obtained by step (1), then
10g brown sugar is added, while supplements 400mL moisture, is packed using loading amount 0.5Kg respiratory membranes, sealing, obtains raw material；
(4) by raw material under the conditions of 28 DEG C, ferment 10d.
Embodiment 3
A kind of production technology for the ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit, which is characterized in that specific packet
Include following steps：
(1) hybrid bacterial strain prepares：Screen excellent aspergillus oryzae, lactobacillus thermophilus, lactobacillus plantarum and candida utili
It carries out tablet to spread cultivation, then Liquid Culture is to 109A/mL；Then according to aspergillus oryzae, lactobacillus thermophilus, lactobacillus plantarum and production protein
The proportioning of Candida is 2：1：1：1 mixing for standby use；
(2) Chinese medicine material prepares：Weigh 444 grams of part ginkgo leaves, 444 grams of cordate houttuynias, 444 grams of Radix Astragalis, 444 grams of radix scutellariaes and
224 grams of selfheals, crushing sieve with 100 mesh sieve spare；
(3) fermentation prepares：By Chinese medicine material after mixing, hybrid bacterial strain 140mL is accessed, and adds 200g brown sugar, together
When keep the skin wet 700mL, packed using loading amount 1Kg respiratory membranes, sealing, obtain raw material；
(4) by raw material under the conditions of 35 DEG C, ferment 15d.
Comparative example 1
A kind of production technology of ginkgo leaf Chinese traditional compound medicine, specifically comprises the following steps：
(1) Chinese medicine material prepares：Weigh 417 grams of part ginkgo leaves, 250 grams of cordate houttuynias, 83 grams of Radix Astragalis, 83 grams of radix scutellariaes and 167
Gram selfheal, crushing sieve with 100 mesh sieve spare；
(2) above-mentioned Chinese medicine material is uniformly mixed and can obtain ginkgo leaf Chinese traditional compound medicine.
A kind of ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit that above-described embodiment 1 is prepared and comparison
The middle medicine composite preparation that example 1 is prepared carries out effect detection.
1 test method
(1) animal is detected：1 age in days healthy AA broiler chicken, 180 plumage of weight close (46.46 ± 1.02g) is selected same beautiful
Rice-bean pulp type basal diet raises experiment broiler chicken, and specific feed formula and Major Nutrient level are shown in Table 1.
Table 1
Note：Premix provides copper Cu 10mg, iron Fe 60mg, manganese Mn 60mg, zinc Zn 120mg, selenium for every kilogram of daily ration
Se 0.3mg, VA 10000IU, VB1 2mg, VB2 5mg, VB6 4mg, VB12 0.04mg, VD3 3500IU, VE 50IU, VK
3IU, D-VB5 calcium 20mg, biotin 0.20mg, niacin 40mg, folic acid 2.0mg；Trophic level is calculated value.
(2) experimental design
Detection animal is randomly divided into 8 groups, respectively blank control group, aureomycin group, 1 low dose group of embodiment, implementation
1 middle dose group of example, 1 high dose group of embodiment, 1 low dose group of comparative example, 1 middle dose group of comparative example, 1 high dose group of comparative example.
Blank control group：Feed Corn-soybean basal diet
Aureomycin group：The feeding aureomycin of 300mg/kg is added in basal diet,
1 low dose group of embodiment：Add what the embodiment 1 that mass percent is 0.25% was prepared in basal diet
A kind of ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit；
1 middle dose group of embodiment：Add what the embodiment 1 that mass percent is 0.50% was prepared in basal diet
A kind of ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit；
1 high dose group of embodiment：Add what the embodiment 1 that mass percent is 1.00% was prepared in basal diet
A kind of ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit；
1 low dose group of comparative example：Add what the comparative example 1 that mass percent is 0.25% was prepared in basal diet
Ginkgo leaf Chinese traditional compound medicine；
1 middle dose group of comparative example：Add what the comparative example 1 that mass percent is 0.50% was prepared in basal diet
Ginkgo leaf Chinese traditional compound medicine；
1 high dose group of comparative example：Add what the comparative example 1 that mass percent is 1.00% was prepared in basal diet
Ginkgo leaf Chinese traditional compound medicine；
(3) testing index
A, growth performance：Experimental period, per weekend, weighs, and consumption weekly is counted as unit of repetition after broiler chicken 12h fasting
Doses calculates average daily gain (ADG), average daily gain (ADFI) and the feed-weight ratio (F/G) in entire raising stage.
B, Immune Organs Index：During 21d and 35d, after broiler chicken fasting 12h, each repetition is chosen 1 chicken and is butchered, and slaughters
Thymus gland, spleen and the bursa of farbricius are detached after government official, using weight as basic Computation immunity shoot formation.Immune Organs Index calculates public：Exempt from
Epidemic disease shoot formation=immune organ fresh weight (g)/weight (g) × 100%.
C, sero-immunity index：During 21d and 35d, after broiler chicken fasting 12h, each 1 chicken progress jugular vein of selection that repeats is adopted
Blood, 2000rpm/min detach serum after centrifuging 5min, and -80 DEG C preserve, and the examination of Bioengineering Research Institute's offer is built up using Nanjing
Agent box measures serum IgG, IgM and IgA contents.
D, Serum antioxidant indices：It is each to repeat to choose 1 chicken progress jugular vein after broiler chicken fasting 12h during 21d and 35d
Blood sampling, 2000rpm/min detach serum after centrifuging 5min, and -80 DEG C of preservations build up Bioengineering Research Institute's offer using Nanjing
Kit measurement soluble transferring receptor (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-
PX), malonaldehyde (MDA) and catalase (CAT) content.
(4) statistical analysis
Each test data carries out single factor test after 2010 edits of Microsoft Excel, using SPSS13.0 softwares
Variance analysis is carried out Multiple range test using Duncan methods, is as a result represented with " average ± standard deviation ".
2nd, experimental result
(1) influence of the ginkgo leaf compound fermentation preparation that the embodiment of the present invention 1 is prepared to meat chicken growth performance, as a result
Such as the following table 2.
Table 2
By data in table 2 it is found that the ginkgo leaf compound fermentation of a kind of enhancing broiler feeding benefit that embodiment 1 is prepared
The middle medicine composite preparation that preparation and comparative example 1 are prepared has no significant effect (P to 0~35d meat chicken growth performances>
0.05).The ginkgo leaf compound fermentation preparation that aureomycin and the embodiment of the present invention 1 are prepared, which can have, improves growth of meat chicken
The trend of energy.Wherein broiler chicken ADG is respectively increased in daily ration addition 300mg/kg aureomycin and 0.25% ginkgo leaf compound fermentation agent
2.80% and 2.12%.Illustrate that the ginkgo leaf compound fermentation agent that the present invention is prepared has excellent raising growth of meat chicken
Energy.
(2) influence result of the ginkgo leaf compound fermentation preparation that the embodiment of the present invention 1 is prepared to broiler chicken immune function
As shown in table 3-4, wherein table 3 show influence of the daily ration addition ginkgo leaf compound fermentation agent to broiler chicken Immune Organs Index.
Table 3
Daily ration adds influence of the ginkgo leaf compound fermentation agent to broiler chicken Immune Organs Index and is shown in Table 3.As shown in Table 3, daily ration
The ginkgo leaf compound fermentation agent that the compound preparation and embodiment 1 that addition comparative example 1 is prepared are prepared is to 21d and 35d meat
Immune Organs of Chicken index has no significant effect (P>0.05).But the ginkgo leaf that daily ration adds aureomycin and embodiment 1 is prepared
Compound fermentation agent has the trend for improving broiler chicken 21d broiler chicken Immune Organs Indexes.Wherein daily ration addition aureomycin and 1.00% silver medal
Apricot leaf compound fermentation agent makes broiler chicken thymus index that 12.82% and 7.69% be respectively increased；Add aureomycin and 0.25% ginkgo
Leaf compound fermentation agent makes broiler chicken index and spleen index that 11.11% and 33.33% be respectively increased；Add aureomycin and 0.25%, 0.50%
Ginkgo leaf compound fermentation agent makes broiler chicken bursal index be respectively increased 20.00%, 10.00% and 10.00%.
Table 4 show influence of the daily ration addition ginkgo leaf compound fermentation agent to serum of broilers immunoglobulin content.
Table 4
Data result is it is found that daily ration addition ginkgo leaf compound fermentation agent can be significantly or extremely aobvious to 21d broiler chicken as shown in Table 4
It writes and improves 21d serum of broilers IgG and IgA contents (P<0.05,0.01), 21d serum IgMs and 35d serum immune globulins are contained
Amount has no significant effect (P>0.05).
Compared with blank control group, aureomycin group and comparative example 1, daily ration, which adds 0.25% ginkgo leaf compound fermentation agent, makes meat
Chicken serum IgG content is significantly or pole significantly improves 40.11% (P<0.05), 49.29% (P<, 33.76% 0.01)~
48.02% (P<0.05 or P<0.01)；Compared with aureomycin group, 0.50% and 1.00% ginkgo leaf compound fermentation agent of addition makes blood
Clear IgG content has been respectively increased can significantly or pole significantly improves serum 25.67% and 22.99%；Blank control group is mould with gold
Plain group and the not notable (P of 1 difference of comparative example>0.05).Compared with blank control group, aureomycin group and comparative example 1, daily ration adds
1.00% ginkgo leaf compound fermentation agent is added to make, and serum of broilers IgA contents are notable or pole significantly improves 35.04% (P<0.05)、
56.78% (P<And 36.03%~58.12% (P 0.01)<0.05 or P<0.01), and with other ginkgo leaf compound fermentation agent groups
Significant difference (P<0.05)；Add 0.25% and 0.50% ginkgo leaf compound fermentation agent and control group and aureomycin group serum IgA
Not notable (the P of content difference>0.05).
(3) influence of the ginkgo leaf compound fermentation agent to serum of broilers anti-oxidation function, the results are shown in Table 5.
Table 5
As shown in Table 5, daily ration addition ginkgo leaf compound fermentation agent, which can improve, significantly improves 21d serum of broilers T-AOC and contains
Amount, significantly reduces Content of MDA (P<0.05,0.01)；35d SOD in serum contents can be significantly improved, significantly reduce serum
MDA contents (P<0.05,0.01).
Compared with blank control group, daily ration, which adds 0.25% ginkgo leaf leavening, makes 21d serum of broilers T-AOC contents notable
Improve 27.94% (P<0.05)；Compared with aureomycin group, the ginkgo leaf compound fermentation agent of daily ration addition various dose is extremely notable
21d serum of broilers T-AOC contents are improved, 59.32% and 49.41% and 59.32% (P has been respectively increased<0.01)；Comparison
Example 1 is close with aureomycin group serum T-AOC contents；21d serum of broilers T-AOC between control group, aureomycin group and comparative example 1
Not notable (the P of content difference>0.05).Compared with blank control group, daily ration, which adds 0.50% ginkgo leaf compound fermentation agent, makes 21d meat
Chicken serum MDA contents pole significantly reduces 32.45% (P<0.01), the MDA contents of blank control group and comparative example 1 are higher；With
Not notable (the P of aureomycin group difference>0.05)；Aureomycin group significantly reduces 40.58% (P than control group MDA contents pole<
0.01), the not notable (P of difference between 1 each dosage component of embodiment>0.05).
Compared with blank control group, daily ration addition various dose ginkgo leaf compound fermentation agent, which has, improves 21d serum of broilers
SOD and GSH-PX contents reduce the trend (P of CAT contents>0.05)；0.50% ginkgo leaf compound fermentation agent group SOD in serum content
Improve 34.11%；1.00% ginkgo leaf compound fermentation agent group serum GSH-PX contents improve the reduction of 21.64%, CAT contents
13.64%.
Compared with blank control group, aureomycin group and comparative example 1, daily ration adds 1.00% ginkgo leaf compound fermentation agent and improves
Or pole significantly improves 35d serum of broilers SOD contents, improves 13.44% (P than blank control group and aureomycin group respectively>
And 65.20% (P 0.05)<0.01)；Compared with aureomycin group, 0.25% and 0.50% ginkgo leaf compound fermentation agent makes broiler chicken blood
39.09% and 57.05% (P has been respectively increased in clear SOD contents<0.05).Compared with blank control group and comparative example 1, daily ration adds
0.50% ginkgo leaf compound fermentation agent is added to significantly reduce MDA contents in 35d serum of broilers, is reduced compared with blank control group
25.34% (P<0.05).
From above-mentioned testing result：
Ginkgo leaf compound fermentation preparation can enhance oxidation resistance, reduce virose mda content, promote to have anti-
The superoxide dismutase and catalase of oxidation are formed, so as to which catalysis is accelerated to remove the oxygen freedom that biological oxygen generates
Base blocks the initiation of lipid peroxidation, makes cell and tissue from damage, MDA contents decrease.This experiment discovery,
With control group, aureomycin group and not plus compared with the compound preparation of ginkgo leaf, 0.50% leavening can significantly inhibit MDA generations, promote
Into the generation of SOD and GSH-PX, so as to enhance oxidation resistance.In conclusion the agent of ginkgo leaf compound fermentation, which has, improves broiler chicken
The trend of production performance, 0.25~0.5% ginkgo leaf compound fermentation agent can enhance the immune function of 21d broiler chicken and anti-oxidant work(
Energy.
IgG is chicken serum main antibody, is played a significant role in body resistant to infection is immunized, and is the important immune finger of body
Mark, serum IgG content raising illustrate that humoral immunity of organism function enhances；Serum globulins include immunoglobulin, horizontal high
It is low to reflect Immunologic States.This experiment is found, is compared with blank control group, aureomycin group and comparative example 1,1.00% hair
Ferment agent can be such that IgG content in chicken serum significantly increases, while IgM is promoted to generate.Illustrate a kind of enhancing that the present invention is prepared
The ginkgo leaf compound fermentation preparation of broiler feeding benefit can enhance broiler chicken immune function.
Each embodiment is described by the way of progressive in this specification, the highlights of each of the examples are with other
The difference of embodiment, just to refer each other for identical similar portion between each embodiment.For device disclosed in embodiment
For, since it is corresponded to the methods disclosed in the examples, so description is fairly simple, related part is said referring to method part
It is bright.
The foregoing description of the disclosed embodiments enables professional and technical personnel in the field to realize or use the present invention.
A variety of modifications of these embodiments will be apparent for those skilled in the art, it is as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, it is of the invention
The embodiments shown herein is not intended to be limited to, and is to fit to and the principles and novel features disclosed herein phase one
The most wide range caused.
Claims (8)
1. a kind of production technology for the ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit, which is characterized in that specifically include
Following steps：
(1) hybrid bacterial strain prepares：Excellent bacterial strain is screened, tablet is first carried out and spreads cultivation, then carry out Liquid Culture, it is spare；
(2) Chinese medicine material prepares：The Chinese medicine material including ginkgo leaf is weighed, it is spare；
(3) fermentation prepares：By above-mentioned Chinese medicine material after mixing, hybrid bacterial strain is accessed, and adds brown sugar, keeps the skin wet, makes
It is packed, sealed with respiratory membrane, obtain raw material；
(4) above-mentioned raw material are subjected to fermentation process.
2. a kind of production technology of ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit according to claim 1,
It is characterized in that, the excellent bacterial strain screened in the step (1) includes aspergillus oryzae, lactobacillus thermophilus, lactobacillus plantarum and production protein
Candida.
3. a kind of production technology of ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit according to claim 2,
It is characterized in that, the proportioning of the aspergillus oryzae, lactobacillus thermophilus, lactobacillus plantarum and candida utili in the step (1) is 2
~4：1~4：1~3：1~2.
4. a kind of production technology of ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit according to claim 3,
It is characterized in that, Liquid Culture is to 10 in the step (1)9~1010A/mL.
5. a kind of production technology of ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit according to claim 4,
It is characterized in that, step (2) Chinese medicine material includes the component of following parts by weight：4~7 parts of ginkgo leaves, 2~4 portions of cordate houttuynias,
1~3 part of Radix Astragali, 1~2 part of radix scutellariae and 2~5 portions of selfheals.
6. a kind of production technology of ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit according to claim 5,
It is characterized in that, the mass ratio of the volume of hybrid bacterial strain and Chinese medicine material is 5~7mL in the step (3)：100g；Brown sugar with
The mass ratio of Chinese medicine material is 8~10：100；Water volume is 35-40mL with Chinese medicine material mass ratio:100g.
7. a kind of production technology of ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit according to claim 6,
It is characterized in that, 0.5~2Kg of respiratory membrane loading amount in the step (3).
8. a kind of production technology of ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit according to claim 7,
It is characterized in that, fermentation temperature is 20~35 DEG C in the step (4), 10~15d of fermentation time.
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| CN201810156340.1A CN108260737A (en) | 2018-02-24 | 2018-02-24 | A kind of production technology for the ginkgo leaf compound fermentation preparation for enhancing broiler feeding benefit |
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| CN108902538A (en) * | 2018-09-17 | 2018-11-30 | 安徽农业大学 | Anti- broiler chicken stress Se-enriched herbal medicine additive, preparation method and application method |
| CN111602629A (en) * | 2020-05-29 | 2020-09-01 | 南宁学院 | Breeding method for improving growth performance of broiler chickens by using fermented Chinese herbal medicines |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108902538A (en) * | 2018-09-17 | 2018-11-30 | 安徽农业大学 | Anti- broiler chicken stress Se-enriched herbal medicine additive, preparation method and application method |
| CN111602629A (en) * | 2020-05-29 | 2020-09-01 | 南宁学院 | Breeding method for improving growth performance of broiler chickens by using fermented Chinese herbal medicines |
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