CN104721337B - Traditional Chinese medicine composition for preventing and treating chicken colibacillosis and preparation method and application thereof - Google Patents
Traditional Chinese medicine composition for preventing and treating chicken colibacillosis and preparation method and application thereof Download PDFInfo
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Abstract
The invention provides a traditional Chinese medicine composition, which comprises the raw materials of 10-30 parts of Chinese pulsatilla root, 3-30 parts of coptis root, 3-30 parts of scutellaria baicalensis and 5-15 parts of myrobalan. The Chinese medicinal composition has good therapeutic effect on intestinal infectious diseases caused by pathogenic Escherichia coli, Salmonella, Pasteurella, etc.
Description
Technical Field
The invention relates to a traditional Chinese medicine composition, in particular to a traditional Chinese medicine composition for treating bacterial diseases of poultry, a preparation method and application thereof.
Background
With the gradual development of the breeding industry of China to scale, intensification and modernization, but problems also exist, for example, the incidence rate of livestock and poultry diseases rises year by year, new diseases are continuously increased, and particularly, the trend that the diseases damaging the safety of livestock and poultry are rapidly spread becomes an important obstacle which troubles the breeding industry of China. Especially the bacterial infection of colibacillus, salmonella, pasteurella, staphylococcus and the like, is the most serious bacterial disease which endangers the breeding production, particularly the production of broiler chickens at present. The morbidity and mortality are high, and serious economic losses are caused. At present, antibacterial and anti-inflammatory western medicines are mainly adopted for treating the chicken colibacillosis, the problems of easy generation of drug resistance and adverse side effects exist although the antibacterial and anti-inflammatory western medicines have certain curative effects, and chemical medicines remained in chicken and eggs also bring potential threats to human health. Modern pharmacological studies have found that: many Chinese herbal medicines have the effects of resisting bacteria, diminishing inflammation, relieving fever, resisting viruses, regulating the immunity of organisms and the like, and also have the advantages of small toxic and side effects, no drug resistance, multi-component synergistic effect and the like, so the research of natural medicines is more and more focused. Is used for preventing and treating diseases of poultry and has wide application prospect. Although the types of the Chinese herbal medicine preparations for treating chicken colibacillosis sold in the market at present are many, the actual using effect is not satisfactory.
The patent with publication number CN101856416A discloses a Chinese herbal medicine oral liquid for treating chicken colibacillosis, which comprises the following raw materials in parts by weight: 4-6 parts of Chinese pulsatilla root, 2-4 parts of coptis root, 2-4 parts of scutellaria baicalensis, 1-3 parts of phellodendron bark, 1-3 parts of ash bark, 1-3 parts of white paeony root, 1-3 parts of dark plum fruit, 1-2 parts of myrobalan, 1-3 parts of codonopsis pilosula, 1-3 parts of astragalus mongholicus and 1-2 parts of liquorice. However, the components in the formula are more, and the quality standard is not easy to control; the components in the formula are more, and the cost is higher; and the antibacterial effect is general.
Disclosure of Invention
Aiming at the problems of drug resistance and drug residue of bacteria to western medicines at present, the invention provides an effective traditional Chinese medicine composition, which can treat poultry diarrhea caused by pathogens such as escherichia coli and the like and environmental factors such as feeding management and the like.
The invention mainly aims to provide a traditional Chinese medicine composition, wherein the raw materials of the traditional Chinese medicine composition comprise 10-30 parts of Chinese pulsatilla root, 3-30 parts of coptis chinensis, 3-30 parts of scutellaria baicalensis and 5-15 parts of myrobalan.
Preferably, the raw materials of the traditional Chinese medicine composition comprise 20-30 parts of Chinese pulsatilla root, 10-30 parts of coptis chinensis, 10-30 parts of scutellaria baicalensis and 10-15 parts of myrobalan.
More preferably, the raw materials of the traditional Chinese medicine composition comprise 30 parts of Chinese pulsatilla root, 30 parts of coptis chinensis, 30 parts of scutellaria baicalensis and 15 parts of myrobalan.
More preferably, the raw materials of the traditional Chinese medicine composition comprise 30 parts of Chinese pulsatilla root, 20 parts of coptis chinensis, 20 parts of scutellaria baicalensis and 15 parts of myrobalan.
Preferably, the traditional Chinese medicine composition is oral liquid, powder and granules.
Another object of the present invention is to provide a method for preparing a Chinese medicinal composition, which comprises: soaking the raw materials of the Chinese medicinal composition of claim 1 in water, and heating for extraction; soaking the extracted raw materials in water, and then heating and extracting; mixing the two extracting solutions, concentrating, precipitating with ethanol, collecting the supernatant, and making into the dosage form of the Chinese medicinal composition.
The preparation method specifically comprises the steps of crushing the pulsatilla chinensis, the coptis chinensis, the scutellaria baicalensis and the myrobalan, sieving the crushed pulsatilla chinensis, the coptis chinensis, the scutellaria baicalensis and the myrobalan through a 20-80-mesh sieve, soaking the raw materials for 6-8 hours by using 3-5 times of distilled water for the first time, then adjusting the pH value to 4.0-5.0 by using a 2% NaOH solution, extracting the raw materials for 6-12 hours in a water bath at 60-80 ℃, filtering the raw materials through 4-6 layers of gauze, adding 3-5 times of distilled water for the second time, extracting the raw materials for 6 hours in a water bath at 60-80 ℃, concentrating the leachate to 1/5 with the volume, placing the leachate in an environment at 4 ℃, precipitating the leachate for 12-24 hours by using 50-90% ethanol with 3-5 times of weight, filtering the leachate through 4-6 layers of gauze, taking supernate, adding 2-5% of gelatin and 2-5% of β -cyclodextrin into the supernate, concentrating the filtrate to obtain a concentrated solution with the relative density of 1.06-1.08.
The invention also aims to provide application of the traditional Chinese medicine composition in preparing medicines for treating and preventing bacterial diseases of poultry.
Preferably, the bacterial diseases of the poultry comprise diseases caused by escherichia coli, salmonella, pasteurella and staphylococcus infections.
Preferably, 100ml of the Chinese medicinal oral liquid is mixed with 25kg of water for 2 times a day for 5 consecutive days.
The invention has the following technical effects:
1. has reasonable formula, can realize synergistic effect and has stronger broad-spectrum antibacterial property.
2. The invention is a pure Chinese medicinal preparation, the myrobalan and the coptis in the prescription have the inhibiting effect on various bacteria, and the medicine has the effects of clearing away heat and toxic materials, astringing intestines and stopping diarrhea by matching with the pulsatilla chinensis, particularly has good curative effect on damp-heat diarrhea, and has obvious treatment effect on diarrhea and watery diarrhea caused by bacteria due to improper feeding and management of escherichia coli, salmonella and the like of poultry. Clinical tests show that the product has a prevention rate of over 90 percent for escherichia coli and a prevention rate of over 97 percent for diseases caused by improper feeding management.
3. The medicinal composition has low toxicity and low cost, can be directly added into drinking water of animals or mixed with materials after being prepared into powder by spray drying through a spray dryer, is convenient to use, can play a role in preventing bacterial diseases, and can enhance the resistance of organisms.
Detailed Description
The present invention will be described in detail with reference to specific examples.
Example 1: preparation of the Chinese medicinal composition oral liquid
The formula of the traditional Chinese medicine composition is shown in the table 1.
Table 1 specific formula of the Chinese medicinal composition
| Chinese medicine | Composition 1 (in parts) | Composition 2 (in parts) | Composition 3 (in parts) |
| Chinese pulsatilla root | 15 | 30 | 30 |
| Coptis root, rhizoma Coptidis | 15 | 30 | 20 |
| Radix Scutellariae | 15 | 30 | 20 |
| Medicine terminalia fruit | 15 | 15 | 15 |
1. The traditional Chinese medicines are weighed according to the specific formula of the traditional Chinese medicine composition in the table 1, and the weight of the components is shown in the table 2.
TABLE 2 weight of each component of the Chinese medicinal composition
| Chinese medicine | Composition 1 (g) | Composition 2 (g) | Composition 3 (g) |
| Chinese pulsatilla root | 100 | 115 | 141.2 |
| Coptis root, rhizoma Coptidis | 100 | 115 | 94.1 |
| Radix Scutellariae | 100 | 115 | 94.1 |
| Medicine terminalia fruit | 100 | 57.5 | 70.6 |
2. Pulverizing radix Pulsatillae, Coptidis rhizoma, Scutellariae radix and fructus Chebulae, and sieving with 60 mesh sieve.
3. Soaking the above composition in 2000ml water beaker for 6 hr, extracting at 80 deg.C for 12 hr, and filtering.
4. 2000ml of water was added to the residue, which was then extracted at 80 ℃ for 6 hours and concentrated to 800 ml. Placing in an environment of 4 deg.C, adding 2400ml of 90% ethanol into the concentrated solution, precipitating with ethanol for 24h, filtering, and collecting supernatant.
5. Collecting supernatant, concentrating under reduced pressure, recovering ethanol, and concentrating to 400 ml.
6. Boiling the concentrated solution of 5 above, adding 1% Tween 80, adding 5%101 clarifier 32ml, 0.5% nipagin, mixing, vacuumizing, filtering with 0.65 μm filter membrane, bottling, and sterilizing.
Example 2: preparation of Chinese medicinal composition powder
1. The traditional Chinese medicines were weighed according to the specific formulation of the traditional Chinese medicine composition in table 1.
2. Pulverizing radix Pulsatillae, Coptidis rhizoma, Scutellariae radix and fructus Chebulae, and sieving with 60 mesh sieve.
3. Soaking the above composition in 2000ml distilled water beaker for 6 hr, extracting in 80 deg.C water bath for 12 hr, and filtering.
4. 2000ml of distilled water was added to the residue, which was then extracted in a water bath at 80 ℃ for 6 hours and concentrated to 800 ml. Placing in 4 deg.C environment, adding 2400ml 90% ethanol into the concentrated solution, precipitating with ethanol for 24h, filtering with 6 layers of gauze, and collecting supernatant.
5. Collecting supernatant, concentrating under reduced pressure, recovering ethanol, and concentrating to 400 ml.
7. Spray drying with vacuum spray dryer, and packaging.
Example 3: preparation of Chinese medicinal composition granule
1. The traditional Chinese medicines were weighed according to the specific formulation of the traditional Chinese medicine composition in table 1.
2. Pulverizing radix Pulsatillae, Coptidis rhizoma, Scutellariae radix and fructus Chebulae, and sieving with 60 mesh sieve.
3. Soaking the above composition in 2000ml distilled water beaker for 6 hr, extracting in 80 deg.C water bath for 12 hr, and filtering with 4 layers of gauze.
4. 2000ml of distilled water was added to the residue, which was then extracted in a water bath at 80 ℃ for 6 hours and concentrated to 800 ml. Placing in 4 deg.C environment, adding 2400ml 90% ethanol into the concentrated solution, precipitating with ethanol for 24h, filtering with 6 layers of gauze, and collecting supernatant.
5. Collecting supernatant, concentrating under reduced pressure, recovering ethanol, and concentrating to 400 ml.
6. Adding 2% of gelatin and 2% of β -cyclodextrin into the concentrated solution in the step 5.
7. Spray drying with a vacuum spray dryer.
8. 9g of lactose and 0.2g of magnesium stearate are added into the traditional Chinese medicine composition powder.
9. Mixing, tabletting, crushing in a swing granulator, grading, and sieving with 30 mesh sieve to remove fine powder to obtain uniform granule.
Example 4: curative effect test of artificially infected chicken colibacillus
1 preparation of bacterial liquid
Selecting rejuvenated Escherichia coli O78(purchased from China institute of veterinary medicine), inoculating to meat soup culture medium, culturing at 37 deg.C for 24 hr, counting viable bacteria, and making into bacteria with concentration of 1 × 109cfu/ml of bacterial liquid.
Grouping of Experimental animals
After the experimental chickens are raised to 24 days old and the abnormal phenomenon of body conditions is checked, the experimental chickens are randomly divided into 20 groups of traditional Chinese medicine composition 1 (a first group), traditional Chinese medicine composition 2 (a second group), traditional Chinese medicine composition 3 (a third group), ofloxacin medicine control group (a fourth group), anemone powder control group (a fifth group), negative control group (a sixth group) and healthy control group (a seventh group).
3 counteracting toxic pathogen and administering
Except for a healthy control group, 0.2 ml/one of the bacteria liquid is injected into the pectoral muscles of other groups of chickens, 0.5ml of the oral liquid in 1g/ml of the traditional Chinese medicine composition 1 is irrigated to a first group, 0.5ml of the oral liquid in 1g/ml of the traditional Chinese medicine composition 2 is irrigated to a second group, 0.5ml of the oral liquid in 1g/ml of the traditional Chinese medicine composition 3 is irrigated to a third group, and the oral liquid is irrigated to the third group for 1 time in the morning and afternoon and is continuously used for 5 days.
The western medicine control group is administered with enrofloxacin in an amount of 50mg per 1L water. 2 times per day, and using 5d (refer to veterinary drug administration guide chemical paper) and Chinese medicinal preparation control group for administration of 1g (crude drug)/ml and 0.5ml (refer to veterinary drug administration guide Chinese medicinal paper).
4 determination of therapeutic Effect
Onset of disease: the test chicken successively appeared with depressed spirit, loss of appetite, squat or crowding into piles, and two wings drooping, and the disease was judged to be onset.
Grading symptoms: the clinical symptom scoring criteria are shown in table 3.
TABLE 3 clinical symptom scoring criteria
| Rank of | Score of | Symptoms and signs |
| First stage | 0 | The chickens have good mental state and normal drinking water and appetite; the behavioral activities were normal. |
| Second stage | 1 | Crouching or crowding into piles, two wings droop, and the wings quickly move away after being driven. |
| Three-stage | 2 | The patient is in a poor posture, has a faint and drowsy eyes and has a slow response after being dispelled. |
| Four stages | 3 | Extreme depression and expelling of mental depression will not respond, and the patient will not get up or die in lying down. |
Observation indexes are as follows: through mortality, cure rate, effective rate and relative weight gain rate
Mortality rate: in the test period, all dead chickens have typical symptoms of escherichia coli through the autopsy, livers of the dead chickens are aseptically collected and are subjected to differential culture by a Mackanka culture medium, the chickens with the escherichia coli are separated and judged to be dead chickens with the escherichia coli, and the death rate is calculated according to the ratio of the number of the dead chickens with the escherichia coli to the number of the chickens in the whole test group.
The cure rate is as follows: the treatment group, chicken essence state and appetite return to normal, symptoms disappear, score 0, aseptically collect chicken liver, identify and culture chicken without separating Escherichia coli by Mackanka culture medium, and judge to be cured. The cure rate was calculated according to the ratio of the number of cured chickens in the whole test group.
The effective rate is as follows: after the medicine is taken, the spirit and the appetite of the test chickens are obviously improved, the symptoms are relieved, the score reduction is judged to be effective by taking the score recording result of each chicken before the medicine is taken and the score recording result of each chicken at the end of the test as more than or equal to 1, and the effective rate is calculated according to the ratio of the number of the effective chickens to the number of the chickens in the whole test group.
Relative rate of weight gain: and calculating the average weight gain according to the weight of each chicken at the beginning and the end of the test, and then calculating the average weight gain of each group of chickens, wherein the weight gain of the blank control group is 100%, and the weight gain of each group is compared with the weight gain of each group to obtain the relative weight gain rate of each group.
Calculating the formula:
5 results of the test
As can be seen from table 4, after 24h of challenge, the incidence rates of the groups (1-6) were 90%, 91.2%, 89.8%, 91.8%, 90.2%, and 91.7%, and the differences between the groups were not significant. The traditional Chinese medicine composition can effectively reduce the death rate of chicken flocks, and the effective rate of the traditional Chinese medicine composition 2 reaches 95%. In composition 2, the relative weight gain of the chicken flocks is increased by 19.19 percent compared with that of the negative control group.
TABLE 4 influence of Chinese medicinal composition on the treatment effect of chicken colibacillosis
Note: the shoulder marks different lower case letters indicate significant difference (P < 0.05).
Conclusion 6
Different traditional Chinese medicine compositions in the invention can obviously reduce the mortality rate of chicken flocks infected with escherichia coli and improve the weight gain rate of the chicken flocks. And (4) conclusion: the oral liquid of the traditional Chinese medicine composition 2 with 0.5ml is administrated by drenching for 1 time respectively in the morning and afternoon, and has certain treatment effect on colibacillosis after being taken for 5 days.
Example 5: test of treatment of chicken colibacillus by traditional Chinese medicine composition oral liquid
Test method 1
2000 naturally affected 37-day-old chickens were selected as broilers, and divided into 5 groups of 400 chickens. The first group is the high dose group of formula No. 2 in example 1, and the administration method is to add 25kg of water into 200ml of Chinese medicinal oral liquid, 2 times a day, and take 5 days continuously.
Group 2 is the dosage group of formula 2 in example 1, and the dosage is 100ml of Chinese medicinal oral liquid mixed with 25kg of water, 2 times a day for 5 days.
Group 3 is the low dose group of formulation No. 2 in example 1; the application method comprises adding 50ml of Chinese medicinal oral liquid into 25kg of water, and taking 2 times a day for 5 days.
The group 4 western medicine control group is administered enrofloxacin in an amount of 50mg per 1L water. 2 times a day, and using 5 days (refer to veterinary drug use guideline chemical medicine volume),
the Chinese medicinal preparation of group 5 is administered by drenching radix Pulsatillae powder 1g/ml and 0.5ml (referring to the use of Chinese medicinal rolls of south America as veterinary drug).
The clinical symptoms, cure rate and mortality rate of the chicken flocks are observed.
2 test results and analysis
Clinical symptoms: initially, mental depression, diarrhea, abdominal distension and sporadic death. C, performing dissection to examine pathological changes: yellow liquid is in the abdomen, the duodenal intestinal wall becomes thin, and a layer of cellulose membrane is arranged on the surface of the liver. Collecting chicken liver, and identifying and culturing to separate out Escherichia coli by Mackanka culture medium, wherein Escherichia coli can be identified.
It is known that colibacillosis occurs in chickens due to improper climate and environmental feeding management.
As can be seen from Table 5, the cure rate of the traditional Chinese medicine oral liquid of the traditional Chinese medicine composition 2 of the invention on chicken escherichia coli reaches 97-99%, and the mortality rate of chicken flocks can be effectively reduced.
TABLE 5 Effect of Chinese medicinal composition granules on the treatment Effect of Chicken Escherichia coli
3 conclusion
In the formula No. 2 in the example 1, the dosage group is used by adding 25kg of water into 100ml of the traditional Chinese medicine oral liquid, 2 times a day and continuously using for 5 days, has certain treatment effect on colibacillosis of chicken flocks, and the effect is better than that of an enrofloxacin control group and a anemone powder control group.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (6)
1. A Chinese medicinal composition for preventing and treating chicken colibacillosis is provided,
the raw materials of the traditional Chinese medicine composition comprise 15 parts of Chinese pulsatilla root, 15 parts of coptis root, 15 parts of scutellaria baicalensis and 15 parts of myrobalan; or the raw materials of the traditional Chinese medicine composition comprise 30 parts of Chinese pulsatilla root, 30 parts of coptis chinensis, 30 parts of scutellaria baicalensis and 15 parts of myrobalan; the traditional Chinese medicine composition is prepared from 30 parts of Chinese pulsatilla root, 20 parts of coptis chinensis, 20 parts of scutellaria baicalensis and 15 parts of myrobalan.
2. The Chinese medicinal composition according to claim 1, wherein the Chinese medicinal composition is oral liquid, powder or granule.
3. A preparation method of a traditional Chinese medicine composition for preventing and treating chicken colibacillosis comprises the following steps:
soaking the raw materials of the Chinese medicinal composition of claim 1 in water, and heating for extraction;
soaking the extracted raw materials in water, and then heating and extracting;
mixing the two extracting solutions, concentrating, precipitating with ethanol, collecting the supernatant, and making into the dosage form of the Chinese medicinal composition.
4. The use of the Chinese medicinal composition of claim 1 in the preparation of a medicament for treating and preventing chicken colibacillosis.
5. The use of the Chinese medicinal composition of claim 2 in the preparation of a medicament for treating and preventing chicken colibacillosis.
6. The use of claim 5, wherein the oral liquid is prepared by mixing 100ml of the Chinese medicinal composition with 25kg of water for 2 times a day for 5 consecutive days.
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| CN109568560A (en) * | 2019-01-28 | 2019-04-05 | 孟繁华 | A kind of preparation method of active compound that treating the infection of Escherichia coli persister |
| CN112168888A (en) * | 2020-11-12 | 2021-01-05 | 邓云波 | Pure traditional Chinese medicine composition powder for treating bacterial and viral intestinal diseases of livestock and poultry |
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