CN103877521A - Pharmaceutical composition for preventing and treating chicken colibacillosis and preparation method of pharmaceutical composition - Google Patents
Pharmaceutical composition for preventing and treating chicken colibacillosis and preparation method of pharmaceutical composition Download PDFInfo
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- CN103877521A CN103877521A CN201410153295.6A CN201410153295A CN103877521A CN 103877521 A CN103877521 A CN 103877521A CN 201410153295 A CN201410153295 A CN 201410153295A CN 103877521 A CN103877521 A CN 103877521A
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Abstract
The invention discloses a pharmaceutical composition for preventing and treating chicken colibacillosis and a preparation method of the pharmaceutical composition. The composition comprises the following components in parts by weight: 25-30 parts of Chinese pulsatilla roots, 25-30 parts of coptis chinensis, 25-30 parts of scutellaria baicalensis, 15-20 parts of cortex fraxini, 15-20 parts of common andrographis herbs, 15-20 parts of radix isatidis, 10-15 parts of radix curcumae, 10-15 parts of agastache rugosus and 25-30 parts of liquorice. The composition can be used for effectively inhibiting chicken colibacillosis, increasing the protection rate of broiler chicken not suffering from diseases by 5% and reducing the death rate of laying hens suffering from diseases by 23.4%.
Description
Technical field
The present invention relates to a kind of pharmaceutical composition of preventing and treating chicken colibacillosis and preparation method thereof, belong to Chinese medicine veterinary drug technical field.
Background technology
Chicken colibacillosis is a kind of common opportunistic illnesses being caused by Escherichia coli.This disease can be raw in various age in days chicken mass-sendings, show as the different symptoms such as fibrinous pericarditis, fibrin perihepatitis, enteritis, granuloma, peritonitis, inflammation of the umbilical cord, salpingitis, ophthalmia, can have a strong impact on the growth promoter and the egg production that becomes chicken of chickling, cause very large loss to poultry husbandry, restricting the development of China's poultry husbandry.
Colibacillary serotype is more, uses often poor effect of vaccine control; Western medicine control not only brings the residual problem of medicine, also can promote antibacterial to produce drug resistance.Modern poultry husbandry is in scale, standardization, Ecological Development process; the probability that turns the generation stressors such as group, immunity increases greatly; chicken in high production status is extremely sensitive to various stressors; when irriate, very easily there is colibacillosis; feed conversion rate reduces; the speed of growth slows down or laying rate declines, and affects the benefit of raising chicken, has hindered the development of poultry husbandry.
Herbal mixture control chicken colibacillosis, has overcome the shortcoming of Western medicine and vaccine control chicken colibacillosis, and can promote chicken body to eliminate the toxin that escherichia coli are discharged, and avoids it to the harm of chicken body, therefore has broad application prospects.
Summary of the invention
The object of this invention is to provide a kind of pharmaceutical composition of preventing and treating chicken colibacillosis, described pharmaceutical composition can effectively prevent chicken colibacillosis.
In order to realize object of the present invention, inventor provides following technical scheme:
A kind of Chinese medicine composition of preventing and treating chicken colibacillosis, in weight portion, the composition of described pharmaceutical composition comprises: 25～30 parts of the Radix Pulsatillaes, 25～30 parts of Rhizoma Coptidis, 25～30 parts of Radix Scutellariaes, 15～20 parts of Cortex Fraxinis, 15～20 parts of Herba Andrographis, 15～20 parts of Radix Isatidis, 10～15 parts of Radix Curcumaes, 10～15 parts of Herba Pogostemoniss, 25～30 parts, Radix Glycyrrhizae.
The Chinese medicine composition of above-mentioned control chicken colibacillosis, in weight portion, the composition of described pharmaceutical composition comprises: 30 parts of the Radix Pulsatillaes, 30 parts of Rhizoma Coptidis, 30 parts of Radix Scutellariaes, 20 parts of Cortex Fraxinis, 20 parts of Herba Andrographis, 20 parts of Radix Isatidis, 15 parts of Radix Curcumaes, 15 parts of Herba Pogostemoniss, 30 parts, Radix Glycyrrhizae.
The present invention also provides the preparation method of the Chinese medicine composition of above-mentioned control chicken colibacillosis simultaneously.
The preparation method of the pharmaceutical composition of above-mentioned control chicken colibacillosis, in weight portion, by 25～30 parts of the Radix Pulsatillaes, 25～30 parts of Rhizoma Coptidis, 25～30 parts of Radix Scutellariaes, 15～20 parts of Cortex Fraxinis, 15～20 parts of Herba Andrographis, 15～20 parts of Radix Isatidis, 10～15 parts of Radix Curcumaes, 10～15 parts of Herba Pogostemoniss, 25～30 parts of pulverizing of Radix Glycyrrhizae, cross 80 mesh sieves, mix homogeneously, to obtain final product.
The preparation method of the pharmaceutical composition of above-mentioned control chicken colibacillosis, in weight portion, by 30 parts of the Radix Pulsatillaes, 30 parts of Rhizoma Coptidis, 30 parts of Radix Scutellariaes, 20 parts of Cortex Fraxinis, 20 parts of Herba Andrographis, 20 parts of Radix Isatidis, 15 parts of Radix Curcumaes, 15 parts of Herba Pogostemoniss, 30 parts of pulverizing of Radix Glycyrrhizae, cross 80 mesh sieves, mix homogeneously, to obtain final product.
From Chinese veterinarian's angle, chicken colibacillosis is damp disease, in the time that chicken is subject to stress stimulation, feed nutrition is not enough or infect the external cause actions such as other diseases, causes the positive QI-insufficiency of chicken body, the Wei Bugu of battalion, and exopathogen seizes the opportunity to worm one's way into.The heresy that body impression is damp and hot, injures taste, in epidemic disease poison, invades, and causes intestinal clear QI being mixed up with turbid QI, and water wets and bets and raw having loose bowels.Pathogenic heat impairing the liver, liver is verdigris color, and the large downright bad point of volume syringe needle is scattered on its surface.The evil direct attack nutrient blood of damp and hot poison, can cause chicken sudden death.The visible each organ surface of pathogenic heat infringement internal organs has fibrinous exudate, and serous coat thickens, necrosis of ovary, and egg yolk breaks etc.
The present invention, according to Chinese veterinarian's theory, filters out the herbal mixture compositions of control chicken colibacillosis; Deeply systematically study the performance of clinical symptoms and the mechanism of action of pathogen and the interaction in vitro of Chinese medicine to pathogen of suffering from colibacillosis chicken, studied and there is the herbal mixture that suppresses escherichia coli breeding and eliminate the interior colitoxin of body and clinical symptoms.
The pharmaceutical composition of control chicken colibacillosis of the present invention, Rhizoma Coptidis, Radix Scutellariae, Cortex Fraxini heat clearing and damp drying dysentery relieving, the effect of Herba Andrographis, Radix Isatidis clearing away heat-fire, removing heat from blood, elimination colitoxin, the heat clearing away of Radix Curcumae liver heat removing function of gallbladder promoting, Herba Pogostemonis circulation of qi promoting removing dampness, Radix Glycyrrhizae coordinating the actions of various ingredients in a prescription.
Show through clinical experiment; test group is added compositions of the present invention by 0.05% dosage in basal diet; be 96.2% to the protective rate of chicken colibacillosis; the escherichia coli significance of night blindness enteral reduces (P<0.05), and bacillus bifidus, lactobacillus significance increase (P<0.05).Test group is added compositions of the present invention by 0.1% dosage in basal diet, and the cure rate of chicken colibacillosis is 85.4%.
The specific embodiment
Below in conjunction with specific embodiment, content of the present invention is further described in detail.
The preparation of embodiment 1 pharmaceutical composition
Take 3000 grams of the Radix Pulsatillaes, 3000 grams of Rhizoma Coptidis, 3000 grams of Radix Scutellariaes, 2000 grams of Cortex Fraxinis, 2000 grams of Herba Andrographis, 2000 grams of Radix Isatidis, 1500 grams of Radix Curcumaes, 1500 grams of Herba Pogostemoniss, 3000 grams, Radix Glycyrrhizae, pulverized 80 mesh sieves, mix homogeneously, shady and cool dry place saves backup.
The preparation of embodiment 2 pharmaceutical compositions
Take 2500 grams of the Radix Pulsatillaes, 2500 grams of Rhizoma Coptidis, 2500 grams of Radix Scutellariaes, 1500 grams of Cortex Fraxinis, 1500 grams of Herba Andrographis, 1500 grams of Radix Isatidis, 1000 grams of Radix Curcumaes, 1000 grams of Herba Pogostemoniss, 2500 grams, Radix Glycyrrhizae, pulverized 80 mesh sieves, mix homogeneously, shady and cool dry place saves backup.
Embodiment 3 prevents broiler colibacillosis test
In order to investigate the preventive effect of pharmaceutical composition of the present invention to broiler colibacillosis, inventor has carried out zoopery, specific as follows:
Test method:
1.1 experimental animal
Commercially available 1 aa broiler chicken, feeding and management: raise in cages, carry out newcastle immunity by eye dripping, water way respectively in 7,21 ages in days; Carry out fabricius bursa immunity by drip, water way respectively in 10,17 ages in days.
1.2 animal grouping and processing
7 aa broiler chicken are divided into two groups of I, II at random, 500 every group.II group is added pharmaceutical composition of the present invention by 0.05% dosage in feedstuff, is used in conjunction 14 days, and I group is blank group.
1.3 EXPERIMENTAL DESIGN
In 10 of every group of random selections of 21 age in days, carry out heart blood sampling, then cut open inspection, get rapidly liver, tightly tie with fine rule at ileum and caecum porch, then the caecum of tying is taken off, keep cecal content to be hedged off from the outer world, with anti-pollution.
Carrying out according to a conventional method erythrocyte C3b garland formation rate measures; Build up by Nanjing test kit explanation detection serum, the Liver Superoxide Dismutase Activity (SOD) that Bioengineering Research Institute produces; The aseptic caecum chyme that takes; after physiological saline solution dilution; cultivate with its corresponding selective medium, method that the making of culture medium is introduced with reference to Chen Tianshou (Chen Tianshou. the Manufacture and application [M] of microbiological culture media. Beijing: Chinese agriculture publishing house, 1995:33～34); measure escherichia coli, lactobacillus and bacillus bifidus content in content; cultivate 36h for 37 ℃, carry out counting statistics, 45 when delivering for sale; statistics death toll, calculates protective rate.
Result of the test:
Escherichia coli, bacillus bifidus and lactobacillus number (logCFU/g) and protective rate in table 1 cecal content
Note: the different significant differences that represent of colleague's shoulder motes
Interpretation of result:
Can be found out by result in table 1; compared with blank group; pharmaceutical composition of the present invention has improved erythrocyte C3bR (E-C3bR) rosette rate; serum, liver SOD content are improved; probiotics lactobacillus and bacillus bifidus in intestinal are all had to proliferation function in various degree; and intestinal conditional pathogenic bacterium escherichia coli are had to obvious inhibitory action, and all remarkable (P<0.05) of diversity, protective rate has improved 5%.
Embodiment 4 treats the test of laying hen colibacillosis
In order to investigate the effect of pharmaceutical composition of the present invention to laying hen colibacillosis, inventor has carried out zoopery, specific as follows:
Test method:
1.1 experimental animal
The commercially available 1 blue grey laying hen in age in days sea, feeding and management: raise in cages, carry out newcastle immunity by eye dripping, water way respectively in 7,21 ages in days; Carry out fabricius bursa immunity by drip, water way respectively in 10,17 ages in days.
1.2 strain
Escherichia coli pathogenic strain is by this laboratory isolation identification.
1.3 animal grouping and processing
7 Japanese instar chicklings are divided into three groups of I, II, III at random, are respectively blank group, test group, negative control group, 150 every group.II, III group press Hu Gongzheng introduce method (Hu Gongzheng etc. the drug efficacy study [J] of Chinese herbal medicine to artificial challenge chickling colibacillosis. traditional Chinese veterinary medicine magazine, 2001 (1): 1～2.) carry out artificial colibacillosis infection, in the time that chicken group occurs that material feeding reduces symptom, II is added pharmaceutical composition of the present invention by 0.1% dosage in feedstuff, be used in conjunction 7 days, not medicine feed of III group.
1.4 EXPERIMENTAL DESIGN
In 10 of every group of random selections of 60 age in days, cut open after inspection, tightly tie with fine rule at ileum and caecum porch, then the caecum of tying is taken off, keep cecal content to be hedged off from the outer world, with anti-pollution, be used for measuring content escherichia coli, lactobacillus and bacillus bifidus content, the aseptic caecum chyme that takes, after physiological saline solution dilution, with its corresponding selective medium cultivation, the making of culture medium with reference to Chen Tianshou introduce method (Chen Tianshou. the Manufacture and application [M] of microbiological culture media. Beijing: Chinese agriculture publishing house, 1995:33～34).Cultivate 36h, carry out counting statistics for 37 ℃.When 60 age in days, statistics mortality rate.
Result of the test:
Table 2 has provided the result of the test of laying hen colibacillosis treatment.
Escherichia coli, bacillus bifidus and lactobacillus number (logCFU/g) and mortality rate in table 2 cecal content
Note: same column shoulder motes is different represents that diversity is remarkable
Interpretation of result:
As can be seen from Table 2, the cure rate of test group (1-mortality rate) is 85.4%, apparently higher than negative control group.Compared with negative control group, pharmaceutical composition of the present invention all has proliferation function in various degree to probiotics lactobacillus and bacillus bifidus in intestinal, and intestinal conditional pathogenic bacterium escherichia coli are had to obvious inhibitory action, the mortality rate of test group chicken has reduced by 23.4%, and diversity is remarkable (P<0.05) all.Compared with Normal group, diversity is remarkable (P>0.05) not, and this shows that pharmaceutical composition of the present invention can suppress the infringement of chicken source escherichia coli to body, relatively good to the therapeutic effect of colibacillosis.
In sum; the pharmaceutical composition of control chicken colibacillosis of the present invention can effectively suppress the colibacillary pathogenic effects of Intestinum Gallus domesticus road conditional pathogenic bacterium; can improve 5% to the protective rate of normal broiler; the pharmaceutical composition of control chicken colibacillosis of the present invention can effectively be cured chicken bacillosis, and mortality rate has reduced by 23.4%.
Claims (4)
1. prevent and treat the pharmaceutical composition of chicken colibacillosis for one kind, it is characterized in that, in weight portion, the composition of described pharmaceutical composition comprises: 25～30 parts of the Radix Pulsatillaes, 25～30 parts of Rhizoma Coptidis, 25～30 parts of Radix Scutellariaes, 15～20 parts of Cortex Fraxinis, 15～20 parts of Herba Andrographis, 15～20 parts of Radix Isatidis, 10～15 parts of Radix Curcumaes, 10～15 parts of Herba Pogostemoniss, 25～30 parts, Radix Glycyrrhizae.
2. a kind of pharmaceutical composition of preventing and treating chicken colibacillosis according to claim 1, it is characterized in that, in weight portion, the composition of described pharmaceutical composition comprises: 30 parts of the Radix Pulsatillaes, 30 parts of Rhizoma Coptidis, 30 parts of Radix Scutellariaes, 20 parts of Cortex Fraxinis, 20 parts of Herba Andrographis, 20 parts of Radix Isatidis, 15 parts of Radix Curcumaes, 15 parts of Herba Pogostemoniss, 30 parts, Radix Glycyrrhizae.
3. the preparation method of the pharmaceutical composition of a control chicken colibacillosis as claimed in claim 1, it is characterized in that, in weight portion, by 30 parts of the Radix Pulsatillaes, 30 parts of Rhizoma Coptidis, 30 parts of Radix Scutellariaes, 20 parts of Cortex Fraxinis, 20 parts of Herba Andrographis, 20 parts of Radix Isatidis, 15 parts of Radix Curcumaes, 15 parts of Herba Pogostemoniss, 30 parts of pulverizing of Radix Glycyrrhizae, cross 80 mesh sieves, mix homogeneously, to obtain final product.
4. the preparation method of the pharmaceutical composition of a control chicken colibacillosis as claimed in claim 2, it is characterized in that, in weight portion, by 30 parts of the Radix Pulsatillaes, 30 parts of Rhizoma Coptidis, 30 parts of Radix Scutellariaes, 20 parts of Cortex Fraxinis, 20 parts of Herba Andrographis, 20 parts of Radix Isatidis, 15 parts of Radix Curcumaes, 15 parts of Herba Pogostemoniss, 30 parts of pulverizing of Radix Glycyrrhizae, cross 80 mesh sieves, mix homogeneously, to obtain final product.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410153295.6A CN103877521A (en) | 2014-04-17 | 2014-04-17 | Pharmaceutical composition for preventing and treating chicken colibacillosis and preparation method of pharmaceutical composition |
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| CN201410153295.6A CN103877521A (en) | 2014-04-17 | 2014-04-17 | Pharmaceutical composition for preventing and treating chicken colibacillosis and preparation method of pharmaceutical composition |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105982977A (en) * | 2015-02-02 | 2016-10-05 | 北京大北农动物保健科技有限责任公司 | Medicinal composition containing radix pulsatillae, cortex fraxini, rhizoma coptidis, radix scutellariae, honeysuckle flowers and flos trollii, feed and applications of medicinal composition and feed |
| CN106511565A (en) * | 2015-09-09 | 2017-03-22 | 谭慧敏 | Medicament for treating cholera of chicken fowls |
| CN107412673A (en) * | 2017-07-28 | 2017-12-01 | 田东雄桂牧业有限公司 | A kind of Chinese medicine for being used to treat chicken colibacillosis |
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| CN103463501A (en) * | 2013-09-28 | 2013-12-25 | 青岛殖富园机械有限公司 | Oral solution treating colibacillosis of chickens |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103463501A (en) * | 2013-09-28 | 2013-12-25 | 青岛殖富园机械有限公司 | Oral solution treating colibacillosis of chickens |
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| 张英: "天然功能性竹叶提取物——竹叶黄酮", 《中国食品添加剂》 * |
| 石玉祥 等: "复方中药和益生素联合应用对肉鸡舍", 《家畜生态学报》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105982977A (en) * | 2015-02-02 | 2016-10-05 | 北京大北农动物保健科技有限责任公司 | Medicinal composition containing radix pulsatillae, cortex fraxini, rhizoma coptidis, radix scutellariae, honeysuckle flowers and flos trollii, feed and applications of medicinal composition and feed |
| CN106511565A (en) * | 2015-09-09 | 2017-03-22 | 谭慧敏 | Medicament for treating cholera of chicken fowls |
| CN107412673A (en) * | 2017-07-28 | 2017-12-01 | 田东雄桂牧业有限公司 | A kind of Chinese medicine for being used to treat chicken colibacillosis |
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Application publication date: 20140625 |