CN105287790A - Paederia scandens extract and applications thereof - Google Patents
Paederia scandens extract and applications thereof Download PDFInfo
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Abstract
The present invention provides a paederia scandens extract and applications thereof. According to the present invention, the paederia scandens extract provides important application values in treatment of food retention, diarrhea, dyspepsia and other gastrointestinal tract diseases; and the compound traditional Chinese medicine comprising the paederia scandens extract, dried ginger, radix aconiti lateralis preparata, alisma orientalis and glucose oxidase provides good market prospects in the fields of inhibition and killing of pathogenic microorganisms, treatments of food retention, diarrhea, dyspepsia and other gastrointestinal tract diseases, promotion of animal growth, improvement of feed conversion rate, and the like.
Description
Technical field
The present invention relates to field of veterinary, especially a kind of fevervine extract and application thereof.
Background technology
Along with the development of intensive animal husbandry, the disease of domestic animal also becomes increasingly complex, and the use amount of veterinary drug and antibiotic feed additive also day by day increases.The stable antibiotic of properties is excreted to the drug residue caused after in environment in environment.These residual medicines by animal products directly accumulate in human body or by Environment release in other plant, and finally come together in human body with various approach, cause the drug resistance of normal flora in the chronic toxic effect of human body and body to change.Natural medicinal plants structure is unique, and not easily producing drug resistance, can degrade voluntarily in the environment, do not cause the irreversible breaking to environment, to the relative low toxicity of people and animals, meet the requirement of green ecological animal husbandry to medicine, is the important development direction of following veterinary drug.
Fevervine (Paederiascandens) is Maguireothamnus speciosus fevervine herb, and have another name called Herba Paederiae and smelly rattan, being mainly distributed in the areas such as China's southeastern coast and the Yangtze river basin, is Chinese medicine material.The main component of fevervine comprises iridoid glycoside constituents, triterpenes components, steroidal and methods of glycosides thereof, Phenylpropanoid Glycosides class, flavone compound, volatile oil component and other compositions.Modern pharmacology research proves, fevervine has significant antiinflammatory, analgesia, calmness and reduces the multiple effects such as uric acid.A kind of composition that fevervine is used for feed additive at present in veterinary applications uses.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of fevervine extract.
Another technical problem to be solved by this invention is the application providing above-mentioned fevervine extract.
For solving the problems of the technologies described above, technical scheme of the present invention is:
A kind of fevervine extract, the iridoid glycosides containing 60-99%, is prepared by following method:
(1) fevervine medical material is got, add 8-12 times amount 75% (v/v) alcohol reflux 1-3 hour, filter extracting solution, medicinal residues repeat said extracted process 2-3 time, merge extractive liquid, concentrating under reduced pressure, extremely without alcohol taste, the distilled water adding 2-6 times of medical material amount makes dissolving, centrifugal, obtains supernatant;
(2) D101 macroporous adsorbent resin on supernatant, medical material needs the D101 macroporous resin of 200-400ml after dehydrated alcohol swelling pretreatment with amount of resin than for every 1kg medical material, resin column blade diameter length ratio is 1: 8-12, and loading flow velocity is 1.0-2.0BV/h, and elution flow rate is 4-6BV/h;
(3) after loading, first use distilled water eluting resin column 2BV, discard stream and wear liquid and water elution liquid, then use 20-85% (v/v) ethanol-eluting resin column 6BV, collect the eluent of ethanol, 60 DEG C of concentrating under reduced pressure, dry, obtain fevervine extract.
Preferably, above-mentioned fevervine extract, is prepared by following method:
(1) get fevervine medical material, add 10 times amount 75% (v/v) alcohol reflux 2 hours, filter extracting solution, medicinal residues repeat said extracted process 3 times, merge extractive liquid, 60 DEG C of concentrating under reduced pressure, extremely without alcohol taste, the distilled water adding 5 times of medical material amounts makes dissolving, centrifugal, obtains supernatant;
(2) D101 macroporous adsorbent resin on supernatant, medical material needs the D101 macroporous resin of 300ml after dehydrated alcohol swelling pretreatment with amount of resin than for every 1kg medical material, and resin column blade diameter length ratio is 1: 8, and loading flow velocity is 2.0BV/h, and elution flow rate is 6BV/h;
(3) after loading, first use distilled water eluting resin column 2BV, discard stream and wear liquid and water elution liquid, then use 30% (v/v) ethanol-eluting resin column 6BV, collect the eluent of ethanol, 60 DEG C of concentrating under reduced pressure, dry, obtain fevervine extract.
The application of above-mentioned fevervine extract in the medicine for the preparation for the treatment of gastrointestinal tract of livestock and fowls disease.
A kind of compositions being used for the treatment of gastrointestinal tract of livestock and fowls disease, be made up of above-mentioned fevervine extract, Rhizoma Zingiberis, Radix Aconiti Lateralis Preparata, Rhizoma Alismatis and glucoseoxidase, by its weight parts fevervine extract 5-10 part, Rhizoma Zingiberis 3-10 part, Radix Aconiti Lateralis Preparata 3-15 part, Rhizoma Alismatis 5-10 part, glucoseoxidase 3-10 part.
Preferably, the above-mentioned compositions being used for the treatment of gastrointestinal tract of livestock and fowls disease, by its weight parts fevervine extract 6 parts, Rhizoma Zingiberis 5 parts, Radix Aconiti Lateralis Preparata 10 parts, Rhizoma Alismatis 7 parts, glucoseoxidase 5 parts.
The above-mentioned preparation method being used for the treatment of the compositions of gastrointestinal tract of livestock and fowls disease, concrete preparation process is as follows:
(1) take fevervine extract, Rhizoma Zingiberis, Radix Aconiti Lateralis Preparata and Rhizoma Alismatis by prescription amount to carry out sterilizing, being ground into fine powder and sieving;
(2) add the glucoseoxidase of prescription amount again, mix homogeneously, to obtain final product.
Above-mentioned composition rear component of step (2) mixing in actual production also needs to add appropriate pharmaceutical adjunct, and described pharmaceutical adjunct is including but not limited to following several: starch, sucrose, anhydrous glucose, auxiliary whitening, dextrin, lactose, micropowder silica gel.
Above-mentioned another preparation method being used for the treatment of the compositions of gastrointestinal tract of livestock and fowls disease, concrete preparation process is as follows:
(1) take fevervine extract, Rhizoma Zingiberis, Radix Aconiti Lateralis Preparata and Rhizoma Alismatis by prescription amount to carry out sterilizing, pulverize, mix, obtain crude drug mixed powder;
(2) by crude drug mixed powder, add 8-12 times amount water soaking 30-60 minute, after decocting 60-90 minute, filter and once decocted medicinal liquid and medicinal residues; By the medicinal residues obtained, again add appropriate decocting and endure, filtration obtains secondary and decocts medicinal liquid; Repeat above-mentioned steps, obtain three times and decoct medicinal liquid, by three gained medicinal liquid mixing, being evaporated to concentrated solution relative density is 1.10-1.15;
(3) after concentrated solution lets cool, filters, the glucoseoxidase of prescription amount is added, mix homogeneously and get final product.
Above-mentioned composition also needs to add appropriate sodium benzoate in actual production.
The invention has the beneficial effects as follows:
Above-mentioned fevervine extract has significant application value in the gastroenteropathys such as treatment food stagnation, diarrhoea, dyspepsia, especially remarkable to fungistatic effects such as pasteurellosis bacillus, Salmonella, staphylococcus aureus and Fu Shi dysentery bacteriums; Can be made into pre-mixing agent or mixture etc. by forming herbal mixture to other drug or add corresponding adjuvant, herbal mixture pre-mixing agent can by the form administration of mixed feeding, and mixture is by the mode administration of drinking-water.Be applied to suppression and pathogenic microbe killing, the gastroenteropathy such as treatment food stagnation, dyspepsia and diarrhoea, aspect has good market prospect to promote growth of animal, improve food conversion ratio etc.
When especially carrying out prescription with specific Chinese medicine, the mutual synergism of each component, the compositions that gained is used for the treatment of gastrointestinal tract of livestock and fowls disease can effectively treat the gastroenteropathys such as food stagnation, diarrhoea, dyspepsia, ensure the normal of animal intestines and stomach function, reduce antibiotic use, thus ensure that animal health is grown up.
Detailed description of the invention
Below in conjunction with specific embodiment, technical scheme of the present invention is further described.
Embodiment 1
A kind of fevervine extract, containing the iridoid glycosides of 98.3%, is prepared by following method:
(1) get fevervine medical material, add 10 times amount 75% (v/v) alcohol reflux 2 hours, filter extracting solution, medicinal residues repeat said extracted process 3 times, merge extractive liquid, 60 DEG C of concentrating under reduced pressure, extremely without alcohol taste, the distilled water adding 5 times of medical material amounts makes dissolving, centrifugal, obtains supernatant;
(2) D101 macroporous adsorbent resin on supernatant, medical material needs the D101 macroporous resin of 300ml after dehydrated alcohol swelling pretreatment with amount of resin than for every 1kg medical material, and resin column blade diameter length ratio is 1: 8, and loading flow velocity is 2.0BV/h, and elution flow rate is 6BV/h;
(3) after loading, first use distilled water eluting resin column 2BV, discard stream and wear liquid and water elution liquid, then use 30% (v/v) ethanol-eluting resin column 6BV, collect the eluent of ethanol, 60 DEG C of concentrating under reduced pressure, dry, obtain fevervine extract.
Embodiment 2
A kind of fevervine extract, containing the iridoid glycosides of 89.2%, is prepared by following method:
(1) get fevervine medical material, add 12 times amount 75% (v/v) alcohol reflux 3 hours, filter extracting solution, medicinal residues repeat said extracted process 2 times, merge extractive liquid, concentrating under reduced pressure, extremely without alcohol taste, the distilled water adding 2 times of medical material amounts makes dissolving, centrifugal, obtains supernatant;
(2) D101 macroporous adsorbent resin on supernatant, medical material needs the D101 macroporous resin of 400ml after dehydrated alcohol swelling pretreatment with amount of resin than for every 1kg medical material, and resin column blade diameter length ratio is 1: 10, and loading flow velocity is 1.5BV/h, and elution flow rate is 5BV/h;
(3) after loading, first use distilled water eluting resin column 2BV, discard stream and wear liquid and water elution liquid, then use 20% (v/v) ethanol-eluting resin column 6BV, collect the eluent of ethanol, 60 DEG C of concentrating under reduced pressure, dry, obtain fevervine extract.
Embodiment 3
A kind of fevervine extract, containing the iridoid glycosides of 76.9%, is prepared by following method:
(1) get fevervine medical material, add 8 times amount 75% (v/v) alcohol reflux 1 hour, filter extracting solution, medicinal residues repeat said extracted process 3 times, merge extractive liquid, concentrating under reduced pressure, extremely without alcohol taste, the distilled water adding 6 times of medical material amounts makes dissolving, centrifugal, obtains supernatant;
(2) D101 macroporous adsorbent resin on supernatant, medical material needs the D101 macroporous resin of 200ml after dehydrated alcohol swelling pretreatment with amount of resin than for every 1kg medical material, and resin column blade diameter length ratio is 1: 12, and loading flow velocity is 1.0BV/h, and elution flow rate is 4BV/h;
(3) after loading, first use distilled water eluting resin column 2BV, discard stream and wear liquid and water elution liquid, then use 85% (v/v) ethanol-eluting resin column 6BV, collect the eluent of ethanol, 60 DEG C of concentrating under reduced pressure, dry, obtain fevervine extract.
Embodiment 4
A kind of compositions being used for the treatment of gastrointestinal tract of livestock and fowls disease, be made up of fevervine extract, Rhizoma Zingiberis, Radix Aconiti Lateralis Preparata, Rhizoma Alismatis and glucoseoxidase described in embodiment 1, wherein, fevervine extract 6kg, Rhizoma Zingiberis 5kg, Radix Aconiti Lateralis Preparata 10kg, Rhizoma Alismatis 7kg, glucoseoxidase 5kg.
The above-mentioned preparation method being used for the treatment of the compositions of gastrointestinal tract of livestock and fowls disease, concrete preparation process is as follows:
(1) take fevervine extract, Rhizoma Zingiberis, Radix Aconiti Lateralis Preparata and Rhizoma Alismatis by prescription amount to carry out sterilizing, pulverize, mix, obtain crude drug mixed powder;
(2) by crude drug mixed powder, add 10 times amount water soaking 50 minutes, after decocting 80 minutes, filter and once decocted medicinal liquid and medicinal residues; By the medicinal residues obtained, again add appropriate decocting and endure, filtration obtains secondary and decocts medicinal liquid; Repeat above-mentioned steps, obtain three times and decoct medicinal liquid, by three gained medicinal liquid mixing, being evaporated to concentrated solution relative density is 1.13;
(3) after concentrated solution lets cool, filters, the glucoseoxidase of prescription amount is added, mix homogeneously and get final product.
Embodiment 5
A kind of compositions being used for the treatment of gastrointestinal tract of livestock and fowls disease, be made up of fevervine extract, Rhizoma Zingiberis, Radix Aconiti Lateralis Preparata, Rhizoma Alismatis and glucoseoxidase described in embodiment 1, wherein, fevervine extract 5kg, Rhizoma Zingiberis 10kg, Radix Aconiti Lateralis Preparata 3kg, Rhizoma Alismatis 10kg, glucoseoxidase 3kg.
The above-mentioned preparation method being used for the treatment of the compositions of gastrointestinal tract of livestock and fowls disease, concrete preparation process is as follows:
(1) take fevervine extract, Rhizoma Zingiberis, Radix Aconiti Lateralis Preparata and Rhizoma Alismatis by prescription amount to carry out sterilizing, being ground into fine powder and sieving;
(2) add the glucoseoxidase of prescription amount again, mix homogeneously, to obtain final product.
Embodiment 6
A kind of compositions being used for the treatment of gastrointestinal tract of livestock and fowls disease, be made up of fevervine extract, Rhizoma Zingiberis, Radix Aconiti Lateralis Preparata, Rhizoma Alismatis and glucoseoxidase described in embodiment 1, wherein, fevervine extract 10kg, Rhizoma Zingiberis 3kg, Radix Aconiti Lateralis Preparata 15kg, Rhizoma Alismatis 5kg, glucoseoxidase 10kg.
The above-mentioned preparation method being used for the treatment of the compositions of gastrointestinal tract of livestock and fowls disease, concrete preparation process is as follows:
(1) take fevervine extract, Rhizoma Zingiberis, Radix Aconiti Lateralis Preparata and Rhizoma Alismatis by prescription amount to carry out sterilizing, pulverize, mix, obtain crude drug mixed powder;
(2) by crude drug mixed powder, add 12 times amount water soaking 30 minutes, after decocting 90 minutes, filter and once decocted medicinal liquid and medicinal residues; By the medicinal residues obtained, again add appropriate decocting and endure, filtration obtains secondary and decocts medicinal liquid; Repeat above-mentioned steps, obtain three times and decoct medicinal liquid, by three gained medicinal liquid mixing, being evaporated to concentrated solution relative density is 1.15;
(3) after concentrated solution lets cool, filters, the glucoseoxidase of prescription amount is added, mix homogeneously and get final product.
Embodiment 7
A kind of compositions being used for the treatment of gastrointestinal tract of livestock and fowls disease, be made up of fevervine extract, Rhizoma Zingiberis, Radix Aconiti Lateralis Preparata, Rhizoma Alismatis and glucoseoxidase described in embodiment 1, wherein, fevervine extract 8kg, Rhizoma Zingiberis 4kg, Radix Aconiti Lateralis Preparata 4kg, Rhizoma Alismatis 6kg, glucoseoxidase 7kg.
The above-mentioned preparation method being used for the treatment of the compositions of gastrointestinal tract of livestock and fowls disease, concrete preparation process is as follows:
(1) take fevervine extract, Rhizoma Zingiberis, Radix Aconiti Lateralis Preparata and Rhizoma Alismatis by prescription amount to carry out sterilizing, pulverize, mix, obtain crude drug mixed powder;
(2) by crude drug mixed powder, add 8 times amount water soaking 60 minutes, after decocting 60 minutes, filter and once decocted medicinal liquid and medicinal residues; By the medicinal residues obtained, again add appropriate decocting and endure, filtration obtains secondary and decocts medicinal liquid; Repeat above-mentioned steps, obtain three times and decoct medicinal liquid, by three gained medicinal liquid mixing, being evaporated to concentrated solution relative density is 1.10;
(3) after concentrated solution lets cool, filters, the glucoseoxidase of prescription amount is added, mix homogeneously and get final product.
Embodiment 8
A kind of compositions being used for the treatment of gastrointestinal tract of livestock and fowls disease, be made up of fevervine extract, Rhizoma Zingiberis, Radix Aconiti Lateralis Preparata, Rhizoma Alismatis and glucoseoxidase described in embodiment 1, wherein, fevervine extract 7kg, Rhizoma Zingiberis 9kg, Radix Aconiti Lateralis Preparata 8kg, Rhizoma Alismatis 6kg, glucoseoxidase 8kg.
The above-mentioned preparation method being used for the treatment of the compositions of gastrointestinal tract of livestock and fowls disease, concrete preparation process is as follows:
(1) take fevervine extract, Rhizoma Zingiberis, Radix Aconiti Lateralis Preparata and Rhizoma Alismatis by prescription amount to carry out sterilizing, being ground into fine powder and sieving;
(2) add the glucoseoxidase of prescription amount again, mix homogeneously, to obtain final product.
The each amounts of components of embodiment 2-6 increases according to same ratio or reduces, and the parts by weight relation of each component of gained all belongs to protection scope of the present invention.
The composition principle of Chinese medicine composition of the present invention is:
Fevervine extract effect in the gastroenteropathys such as treatment food stagnation, diarrhoea, dyspepsia.
Rhizoma Zingiberis has warming spleen and stomach for dispelling cold, and recuperating depleted YANG is promoted blood circulation, effect of warming the lung to resolve fluid-retention, and for coldness and pain in the epigastrium, vomiting is had loose bowels, and cold extremities faint pulse, cold fluid-retention is breathed with cough.
Radix Aconiti Lateralis Preparata is used for YANG-depletion collapse, cold extremities faint pulse, sexual impotence, cold womb, trusted subordinate's cold type of pain, and deficiency and coldness is vomited and diarrhoea, YIN-cold edema, yang deficiency diseases caused by exogenous pathogenic factor, arthralgia due to cold-dampnes, carbuncle of yin nature skin infection etc.
Rhizoma Alismatis cures mainly controls dysuria, edema distension, vomiting, dysentery etc.
Glucoseoxidase is a kind of aerobic dehydrogenase, can become gluconic acid and hydrogen peroxide by single-minded oxidation of beta-D-Glucose.Glucoseoxidase has antibacterial, growth promoting function, can reduce pH value in animal gastrointestinal tract, thus suppresses harmful bacteria, promotes the growth and breeding of probiotics, reduces animal diarrhea, improves growth of animal, have the effect of similar acidulant.The use of glucoseoxidase is considered to safe feed additive, and it is the natural product of fermentable, is referred to as " green " additive.At present, the research of glucoseoxidase is mainly contained animal intestinal is grown, the impact of fertility performance, and in the application of food fresh keeping and human medical.
Each component synergism, considerably increases the bacteriostasis of fevervine extract, simultaneously whole compositions Be very effective in promotion poultry length and treatment gastroenteropathy.
Test example 1
Embodiment 1 fevervine extract is to poultry encountered pathogenic bacteria body outer suppressioning test
The solution that fevervine extract is prepared is carried out extracorporeal bacteria inhibitor test to the animal pathogen that the poultry such as pasteurellosis bacillus, Salmonella, staphylococcus aureus and Fu Shi dysentery bacterium are common respectively.By sterile working's code, aseptically, the culture medium flat plate that preparation strain is corresponding, draws each bacterium liquid on Agar Plating, and coating evenly.Subsides add drug susceptability test paper sheet, with aseptic nipper, peaceful the hardening of the scraps of paper are closed closely.Every increment product do 3 parts, results averaged.Contrast with enrofloxacin medicinal liquid simultaneously.37 DEG C of constant incubators use vernier caliper measurement antibacterial circle diameter after cultivating 24h, judge the fungistatic effect of medicinal liquid to pasteurellosis bacillus, Salmonella, staphylococcus aureus and Fu Shi dysentery bacterium, judge sensitivity.
Criterion: inhibition zone is greater than 20mm for extremely responsive, and 15-20mm is Gao Min, and 10-15mm is quick in being, below 10mm is hypoallergenic, is insensitive without inhibition zone.
Result detects and shows: under the concentration of fevervine extract and enrofloxacin is 100ug/ml condition, the bacteriostatic diameter of pasteurellosis bacillus, Salmonella, staphylococcus aureus and Fu Shi dysentery bacterium is all greater than 10mm, demonstrate good bacteriostasis, as table 1-1.
Table 1-1 fevervine extract scraps of paper drug sensitive test result (unit: mm)
Compositions described in embodiment 2 is to poultry encountered pathogenic bacteria body outer suppressioning test
Described in embodiment 2, compositions is mixed with required concentration, carries out extracorporeal bacteria inhibitor test respectively to the animal pathogen that the poultry such as pasteurellosis bacillus, Salmonella, staphylococcus aureus and Fu Shi dysentery bacterium are common.By sterile working's code, aseptically, the culture medium flat plate that preparation strain is corresponding, draws each bacterium liquid on Agar Plating, and coating evenly.Subsides add drug susceptability test paper sheet, with aseptic nipper, peaceful the hardening of the scraps of paper are closed closely.Every increment product do 3 parts, results averaged.Contrast with enrofloxacin medicinal liquid simultaneously.37 DEG C of constant incubators use vernier caliper measurement antibacterial circle diameter after cultivating 24h, judge the fungistatic effect of medicinal liquid to pasteurellosis bacillus, Salmonella, staphylococcus aureus and Fu Shi dysentery bacterium, judge sensitivity.
Criterion: inhibition zone is greater than 20mm for extremely responsive, and 15-20mm is Gao Min, and 10-15mm is quick in being, below 10mm is hypoallergenic, is insensitive without inhibition zone.
Result detects and shows: under described in embodiment 2, the concentration of compositions (in Herba Paederiae extract) and enrofloxacin is 100ug/ml condition, the bacteriostatic diameter of pasteurellosis bacillus, Salmonella, staphylococcus aureus and Fu Shi dysentery bacterium is all greater than 10mm, demonstrate good bacteriostasis, as table 1-2.
Compositions scraps of paper drug sensitive test result (unit: mm) described in table 1-2 embodiment 2
Adopt tube dilution method, compare with enrofloxacin, measure the minimum inhibitory concentration (MIC) having the medicinal liquid of bacteriostatic activity.Described in embodiment 2, compositions is test group, makes a series of continuous doubling dilution in ordinary broth, then get 37 DEG C of shaken cultivation 6h for examination bacterium, rarely get 0.1ml, be inoculated in respectively in each medicine test tube, do without medicine bacterial controls simultaneously, 37 DEG C of cultivation 24h.
Criterion: medicine broth culture is limpid is asepsis growth, is minimum antibacterial degree (MIC) with the least concentration of asepsis growth.
The testing result of minimal inhibitory concentration shows: compositions described in embodiment 2 to pasteurellosis bacillus, Salmonella, staphylococcus aureus and Fu Shi dysentery bacterium 4 kinds of poultry encountered pathogenic bacteria bacterial strains all have good fungistatic effect, all have good fungistatic effect to Gram-positive and gram negative bacteria.Described in visible embodiment 2, compositions has the bacteriostasis of wide spectrum.Result of the test shows that the minimum fungistatic effect concentration of compositions described in embodiment 2 is higher than enrofloxacin group (table 2), antibiotic medicine as an alternative, and its source is natural extensively, side effect is little, it is few to remain, and has good market prospect.
Compositions minimal inhibitory concentration MIC described in table 2 embodiment 2 (unit: ug/ml)
Test example 2
Described in embodiment 2, compositions is tested the impact of Growth Performance of Weaning Piglets
EXPERIMENTAL DESIGN: choose the ablactation of (35 ± 3) age in days, body weight close, normotrophic DLY three way cross piglet 120, be divided into 3 process at random by the male and female principle of correspondence between group, each process 3 repetition, each repetition 10.Process one: basal diet matched group; Process two: the enrofloxacin adding 100mg/kg; Process three: the herbal mixture pre-mixing agent (in Herba Paederiae extract) adding 50mg/kg.All feed after off-test the daily ration consistent with matched group, analytic statistics respectively processes every other day weightening finish, feedstuff-meat ratio, diarrhea rate situation etc.
Feeding and management: test is all carried out under same colony house, same poultry raiser and same management mode, feed mode is timing non-quantitative, and eat up as degree to feed in rear 30min, day feeds 3 times, freely drinks water.On-test with at the end of, carry out empty stomach to pig weigh to be treated to unit, a situation arises to record the feed intake of pig, diarrhoea number of days and diarrhoea degree and other disease in process of the test, calculating average daily gain, daily ingestion amount, feedstuff-meat ratio, diarrhea rate etc.
Table 3 herbal mixture pre-mixing agent is on the impact of piglet growth performance
Note: the completely not identical person of letter of going together in mark represents significant difference (P < 0.05).Lower same.
As shown in table 3, average daily ingestion amount and the average daily gain of 3 groups are followed successively by herbal mixture pre-mixing agent, positive controls (enrofloxacin), blank group from high to low.Compared with blank group, add herbal mixture pre-mixing agent in daily ration and improve average feed intake 7.29%; Average daily gain 14.05%; Feedstuff-meat ratio have dropped 5.92% (p < 0.05).Positive controls, herbal mixture pre-mixing agent significantly reduce diarrhea rate (P < 0.05) compared with blank group.Result shows to add feed intake, the daily gain that herbal mixture pre-mixing agent improves piglet in daily ration, and feedstuff-meat ratio and diarrhea rate are reduced, thus effectively improves the growth performance of piglet.Effect is suitable compared with the matched group of antibiotic enrofloxacin.
Test example 3
Herbal mixture pre-mixing agent is tested the impact of broiler growth performance
EXPERIMENTAL DESIGN: 360 1 age in days yellow plumage broiler chicken, body weight are close, are divided into 3 process at random, each process 6 repetition, each repetition 20 by the male and female principle of correspondence between group.Process one: basal diet matched group; Process two: the enrofloxacin adding 25mg/kg; Process three: the herbal mixture pre-mixing agent adding 100mg/kg.All feed after off-test the daily ration consistent with matched group, analytic statistics respectively processes every other day
Weightening finish, feedstuff-meat ratio, mortality rate situation etc.
Feeding and management: before test, hen house, tank and hopper are carried out disinfection.Adopt totally-enclosed ground flat foster, free choice feeding and drinking-water, 24 h light, carry out routine immunization.Its temperature, humidity, density, ventilation, sterilization, epidemic prevention, feeding standard, feeding frequency, feces clearing times etc. are all identical.Each group be in identical environment and rearing conditions under, observe and note down the diet of chicken group and feces discharges situation, death condition etc., once unusual circumstance processes in time, environment inspection, feed apparatus and place sterilization, periodic replacement bedding and padding, regulating temperature etc.
Table 4 herbal mixture pre-mixing agent is on the impact of growth of meat chicken performance
Note: the completely not identical person of letter of going together in mark represents significant difference (P < 0.05).Lower same.
As shown in table 4, average daily ingestion amount and the average daily gain of 3 groups are followed successively by herbal mixture pre-mixing agent, positive controls (enrofloxacin), blank group from high to low.Compared with blank group, add herbal mixture pre-mixing agent in daily ration and improve average feed intake 1.57%; Average daily gain 11.76%; Feedstuff-meat ratio have dropped 9.09% (p < 0.05).Positive controls, herbal mixture pre-mixing agent significantly reduce death toll (P < 0.05) compared with blank group.Result shows to add feed intake, the daily gain that herbal mixture pre-mixing agent improves broiler in daily ration, and feedstuff-meat ratio and death toll are reduced, thus effectively improves the growth performance of broiler.Effect is suitable compared with the matched group of antibiotic enrofloxacin.
Test example 4
Described in embodiment 2, compositions is to the controlling experiment of salmonellosis
Certain chicken house to break out to arrange white loose stool and the disease for cardinal symptom such as drowsiness June.The young most spirit of disease is depressed, and drop-head neck, closes order necking down, lethargy, and slow-witted standing does not like movable, and fear of cold is afraid of cold, and disorderly, two wings are sagging for fine hair pine; Initial stage, sick chicken only showed poor appetite, did not then eat; Crop is spoken in a low voice inanition, and what have has gas; Diarrhoea, row's white slurry sample feces, perianal is stuck with paste by feces, dry and hard rear formation lump, and blocking anus, utters a scream during defecation; Indivedual severe patient is dead because of dyspnea or acute sepsis; The chicken retarded growth of resistance to mistake, feather is dry, and pine is disorderly; The only a few chicken later stage occurs that foot arthroncus is in walking lamely.Select isolated bacterium colony smear for microscopic examination, be gram-negative without the acapsular dialister bacterium of brood cell, blunt garden, two ends, how to be singlely dispersed in.Get the isolate that plain agar inclined-plane is cultivated 24h cultivate through 37 DEG C, add sterile saline and make bacterium liquid, inhale 50 μ L, make dull and stereotyped agglutination with equivalent S. pullonum polyvalent serum type positive serum and test, occur obvious particle agglutination in 2min, result is judged to be positive reaction.Through being accredited as Salmonella pullorum disease.Ill chicken is daily drinks the water preparation that concentration (in Herba Paederiae extract) that described in embodiment 2, compositions is mixed with is 50ppm, be used in conjunction 7 days.While treatment, thorough disinfection is carried out to hen house and surrounding and sprays, once a day, be used in conjunction 7d.After 2 weeks, chicken group gradually recovers one's health.
Above-mentioned detailed description of this kind of fevervine extract and application thereof being carried out with reference to embodiment; illustrative instead of determinate; several embodiments can be listed according to institute's limited range; therefore in the change do not departed under general plotting of the present invention and amendment, should belong within protection scope of the present invention.
Claims (7)
1. a fevervine extract, is characterized in that: the iridoid glycosides containing 60-99%, is prepared by following method:
(1) fevervine medical material is got, add 8-12 times amount 75% (v/v) alcohol reflux 1-3 hour, filter extracting solution, medicinal residues repeat said extracted process 2-3 time, merge extractive liquid, concentrating under reduced pressure, extremely without alcohol taste, the distilled water adding 2-6 times of medical material amount makes dissolving, centrifugal, obtains supernatant;
(2) D101 macroporous adsorbent resin on supernatant, medical material needs the D101 macroporous resin of 200-400ml after dehydrated alcohol swelling pretreatment with amount of resin than for every 1kg medical material, resin column blade diameter length ratio is 1: 8-12, and loading flow velocity is 1.0-2.0BV/h, and elution flow rate is 4-6BV/h;
(3) after loading, first use distilled water eluting resin column 2BV, discard stream and wear liquid and water elution liquid, then use 20-85% (v/v) ethanol-eluting resin column 6BV, collect the eluent of ethanol, 60 DEG C of concentrating under reduced pressure, dry, obtain fevervine extract.
2. fevervine extract according to claim 1, is characterized in that: prepared by following method:
(1) get fevervine medical material, add 10 times amount 75% (v/v) alcohol reflux 2 hours, filter extracting solution, medicinal residues repeat said extracted process 3 times, merge extractive liquid, 60 DEG C of concentrating under reduced pressure, extremely without alcohol taste, the distilled water adding 5 times of medical material amounts makes dissolving, centrifugal, obtains supernatant;
(2) D101 macroporous adsorbent resin on supernatant, medical material needs the D101 macroporous resin of 300ml after dehydrated alcohol swelling pretreatment with amount of resin than for every 1kg medical material, and resin column blade diameter length ratio is 1: 8, and loading flow velocity is 2.0BV/h, and elution flow rate is 6BV/h;
(3) after loading, first use distilled water eluting resin column 2BV, discard stream and wear liquid and water elution liquid, then use 30% (v/v) ethanol-eluting resin column 6BV, collect the eluent of ethanol, 60 DEG C of concentrating under reduced pressure, dry, obtain fevervine extract.
3. the application of fevervine extract described in claim 1 or 2 in the medicine for the preparation for the treatment of gastrointestinal tract of livestock and fowls disease.
4. one kind is used for the treatment of the compositions of gastrointestinal tract of livestock and fowls disease, it is characterized in that: be made up of fevervine extract, Rhizoma Zingiberis, Radix Aconiti Lateralis Preparata, Rhizoma Alismatis and glucoseoxidase described in claim 1 or 2, by its weight parts fevervine extract 5-10 part, Rhizoma Zingiberis 3-10 part, Radix Aconiti Lateralis Preparata 3-15 part, Rhizoma Alismatis 5-10 part, glucoseoxidase 3-10 part.
5. the compositions being used for the treatment of gastrointestinal tract of livestock and fowls disease according to claim 4, is characterized in that: by its weight parts fevervine extract 6 parts, Rhizoma Zingiberis 5 parts, Radix Aconiti Lateralis Preparata 10 parts, Rhizoma Alismatis 7 parts, glucoseoxidase 5 parts.
6. the preparation method being used for the treatment of the compositions of gastrointestinal tract of livestock and fowls disease described in claim 4 or 5, is characterized in that: concrete preparation process is as follows:
(1) take fevervine extract, Rhizoma Zingiberis, Radix Aconiti Lateralis Preparata and Rhizoma Alismatis by prescription amount to carry out sterilizing, being ground into fine powder and sieving;
(2) add the glucoseoxidase of prescription amount again, mix homogeneously, to obtain final product.
7. the preparation method being used for the treatment of the compositions of gastrointestinal tract of livestock and fowls disease described in claim 4 or 5, is characterized in that: concrete preparation process is as follows:
(1) take fevervine extract, Rhizoma Zingiberis, Radix Aconiti Lateralis Preparata and Rhizoma Alismatis by prescription amount to carry out sterilizing, pulverize, mix, obtain crude drug mixed powder;
(2) by crude drug mixed powder, add 8-12 times amount water soaking 30-60 minute, after decocting 60-90 minute, filter and once decocted medicinal liquid and medicinal residues; By the medicinal residues obtained, again add appropriate decocting and endure, filtration obtains secondary and decocts medicinal liquid; Repeat above-mentioned steps, obtain three times and decoct medicinal liquid, by three gained medicinal liquid mixing, being evaporated to concentrated solution relative density is 1.10-1.15;
(3) after concentrated solution lets cool, filters, the glucoseoxidase of prescription amount is added, mix homogeneously and get final product.
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| CN108125070A (en) * | 2018-01-16 | 2018-06-08 | 佛山市所能网络有限公司 | A kind of anti-trip fish for cats bait and preparation method thereof |
| CN112057896A (en) * | 2020-09-17 | 2020-12-11 | 重庆市中药研究院 | Paederia scandens extract and preparation method and application thereof |
| CN115137676A (en) * | 2021-03-30 | 2022-10-04 | 海南师范大学 | Paederia scandens extract and application thereof in preparing toothpaste |
| CN117949591A (en) * | 2023-12-15 | 2024-04-30 | 海南大学 | Method for rapidly screening uric acid-reducing substances in fevervine and application |
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| CN106212516A (en) * | 2016-07-21 | 2016-12-14 | 陈博 | A kind of compound method of biologic formulation antibacterial mixed liquor |
| CN106035423A (en) * | 2016-08-03 | 2016-10-26 | 覃燕兰 | Toy disinfection solution |
| CN108125070A (en) * | 2018-01-16 | 2018-06-08 | 佛山市所能网络有限公司 | A kind of anti-trip fish for cats bait and preparation method thereof |
| CN112057896A (en) * | 2020-09-17 | 2020-12-11 | 重庆市中药研究院 | Paederia scandens extract and preparation method and application thereof |
| CN115137676A (en) * | 2021-03-30 | 2022-10-04 | 海南师范大学 | Paederia scandens extract and application thereof in preparing toothpaste |
| CN115137676B (en) * | 2021-03-30 | 2024-03-26 | 海南师范大学 | Paederia scandens extract and application thereof in preparing toothpaste |
| CN117949591A (en) * | 2023-12-15 | 2024-04-30 | 海南大学 | Method for rapidly screening uric acid-reducing substances in fevervine and application |
| CN117949591B (en) * | 2023-12-15 | 2024-10-11 | 海南大学 | Method for rapidly screening uric acid-reducing substances in fevervine and application |
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