CN106212516A - A kind of compound method of biologic formulation antibacterial mixed liquor - Google Patents
A kind of compound method of biologic formulation antibacterial mixed liquor Download PDFInfo
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- CN106212516A CN106212516A CN201610602894.0A CN201610602894A CN106212516A CN 106212516 A CN106212516 A CN 106212516A CN 201610602894 A CN201610602894 A CN 201610602894A CN 106212516 A CN106212516 A CN 106212516A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Biotechnology (AREA)
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- Mycology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Inorganic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses the compound method of a kind of biologic formulation antibacterial mixed liquor, become including by the mixed raw material of following portions by weight: 25% peroxidase 200～400 parts, glacial acetic acid 2～5 parts, Fructus Forsythiae extracting solution 2～4 parts, Radix Sophorae Flavescentis extractive liquid 1～3 parts, Caulis Spatholobi extracting solution 2～3 parts, carbamide 5～10 parts, heteroauxing 0.01～0.05 part, glucoseoxidase 100～200 parts, the salt 50 of organic acid and/or organic acid～150 parts, Loprazolam 10～20 parts, fumaric acid 5～20 parts, the barium hydroxide 0 of 50%～5 parts.The mixed liquor bactericidal effect persistent period of antibacterial of the present invention is long, bactericidal effect is obvious, skin and histoorgan impact on human body are little, reduce the infringement of the skin to medical worker during use, alleviate the pain of patient when the mixed liquor of antibacterial contacts the skin of patient when.
Description
Technical field
The present invention relates to the preparation method of a kind of biologic formulation antibacterial, particularly a kind of biologic formulation antibacterial mixed liquor
Compound method.
Background technology
At present, in medicinal therapeutic process, sterilization usual for skin degerming cleaning liquid have 3% concentration hydrogen peroxide,
75% concentration ethanol and iodine tincture, wherein iodine tincture is the liquid formed in the alcoholic solution that iodine is dissolved in potassium iodide.Specifically
Using method be to dip bottled above-mentioned sterilization cleaning liquid with common plastics cotton swab, then to skin, wound disinfection or to vessel
Deng equipment disinfection.During routine use, if improper use, used cotton swab is placed again in sterilization cleaning liquid bottle,
Cross infection will be caused.The disinfective action of these conventional sterilization disinfectant not only persistent period is not grown and to skin or mucosa
Tissue irritation is big, with clean a wound, at skin ulceration, at mucosal infections, patient can be produced zest pain and damage
Wound, causes the misery being difficult to bear to patient.Along with the development of society, the raising of people's health care consciousness, people are to external
The requirement of disinfectant also improves constantly, and design studies goes out a kind of novel medical bactericide and has important practical significance.
Summary of the invention
It is an object of the invention to overcome the weak point of above-mentioned conventional art, invention a kind of biologic formulation antibacterial mixing
The compound method of liquid.
For solving the problems referred to above, the technical solution adopted in the present invention is: joining of a kind of biologic formulation antibacterial mixed liquor
Method processed, it is characterised in that: include being become by the mixed raw material of following portions by weight:
25% peroxidase 200～400 parts, glacial acetic acid 2～5 parts, Fructus Forsythiae extracting solution 2～4 parts, Radix Sophorae Flavescentis extractive liquid 1～3
Part, Caulis Spatholobi extracting solution 2～3 parts, carbamide 5～10 parts, heteroauxing 0.01～0.05 part, glucoseoxidase 100～200
Part, the salt 50 of organic acid and/or organic acid～150 parts, Loprazolam 10～20 parts, fumaric acid 5～20 parts, the hydrogen-oxygen of 50%
Change barium 0～5 parts；
Described organic acid and/or the salt of organic acid, heteroauxing, glucoseoxidase, Loprazolam, fumaric acid are at ice
Water-bath mixes, maintains the temperature at-5～15 DEG C of reactions and stir 5～15 minutes, being subsequently adding 25% peroxidase, ice second
Acid, the mixing of Fructus Forsythiae extracting solution, Radix Sophorae Flavescentis extractive liquid, Caulis Spatholobi extracting solution, carbamide, stand 24～48 hours at-5～25 DEG C,
Mixed liquor to antibacterial；
The barium hydroxide of addition 50% regulates the pH value of the mixed liquor of this antibacterial.
A kind of improvement as technique scheme: described by Caulis Spatholobi boiling, described amount of water is made a living the 4 of dose
～6 times, the filtrate being filtrated to get is concentrated into be equivalent to biological weight 1～2 times, respectively obtains the extracting solution of Caulis Spatholobi.
A kind of improvement as technique scheme: the described Fructus Forsythiae through preliminary cleaning treatment, carries out carbon dioxide and surpasses
Critical extraction, described extraction pressure is 20-25Mpa, and Extracting temperature is 45-50 DEG C, and carbon dioxide flow rate is 15-20L/h, separates
Pressure is 15-20Mpa, and separation temperature is 30-45 DEG C, and the time is 4-6 hour, and products therefrom is Fructus Forsythiae extracting solution.
A kind of improvement as technique scheme: described Radix Sophorae Flavescentis is carried out, and after carrying out slicing treatment, put into dense
After degree is for soaking 15-48 hour in the ethanol of 80%-97%, pull the Radix Sophorae Flavescentis after immersion out, ethanol solution slow fire is heated 30-45
Stop working after minute, obtain Radix Sophorae Flavescentis extractive liquid.
A kind of improvement as technique scheme: described organic acid is selected from citric acid, lactic acid, malic acid, succinum
Acid, the acid of tartaric acid at least a part of which more than a kind.
A kind of improvement as technique scheme: the mixed liquor PH of described antibacterial is at PH5.5～PH4.5.
Owing to have employed technique scheme, compared with prior art, the mixed liquor bactericidal effect of antibacterial of the present invention is held
The continuous time is long, and bactericidal effect is obvious, and skin and histoorgan impact on human body are little, reduce medical worker during use
The infringement of skin, when the mixed liquor of antibacterial contacts the skin of patient when, alleviate the pain of patient.
Peroxisome is that diameter is about 0.5～1.0 μm, generally than mitochondrion by the vesicle of one layer of unit membrane parcel
Little.Being prevalent in Eukaryotic various types of cells, in hepatocyte and nephrocyte, quantity is the most.Peroxisome
Marker enzyme is catalase, and hydrogen peroxide is mainly hydrolyzed by its effect.Hydrogen peroxide (H2O2) is oxidase catalyzed
The cytotoxic substance produced in redox reaction, oxidase and catalase be all present in peroxisome, from
And cell is shielded.This characteristic of peroxidase can play the protective effect to contactee.
Glacial acetic acid, i.e. anhydrous acetic acid, organic compound.It is frozen into ice-like when low temperature, is commonly called as glacial acetic acid.Body during solidification
Long-pending expansion may cause vessel breaks.Flash-point 39 DEG C, explosion limit 4.0%～16.0%, in air, maximum acceptable concentration does not surpasses
Cross 25mg/m3.Pure acetic acid can be frozen into ice-like crystal when less than fusing point, so anhydrous acetic acid is also called glacial acetic acid.Conventional
Analytical reagent, is widely used in neutralization or acidization.
Glucoseoxidase is the enzyme found from the mycetes such as Penicllium notatum (Penicillium notatum) and Mel.
It can be catalyzed the reaction of D-Glucose+O2D-gluconic acid (6-lactone)+H2O2.EC1.1.3.4. Penicllium notatum
(P.notatum) enzyme shows with it has antibiotic property to introduce attention.Therefore, also there is the address of glucose oxidase (notatin), existing
The clearest, antibiotic property is that the H2O2 generated due to reaction has sterilization functions.Purify the FAD that thing contains 2 molecules, be subject to as electronics
Body, in addition to O2, also can with 2,6, Dichlorophenol, indophenol reaction.This enzyme has specificity to glucose.The lowest (the 10-of Michaelis constant Km
3M degree), owing to H2O2 can be generated quantitatively, therefore extensively it is applied to biochemistry as the quantitative reagent of D-Glucose
Field and clinical examination.
Detailed description of the invention
Embodiment 1
A kind of compound method of biologic formulation antibacterial mixed liquor, it is characterised in that: include by following portions by weight is former
Material is mixed:
25% peroxidase 200 parts, glacial acetic acid 2 parts, Fructus Forsythiae extracting solution 2 parts, Radix Sophorae Flavescentis extractive liquid 1 part, Caulis Spatholobi is extracted
Liquid 2 parts, 5 parts of carbamide, heteroauxing 0.01 part, glucoseoxidase 100 parts, the salt of organic acid and/or organic acid 50 parts, first
Alkyl sulfonic acid 10 parts, fumaric acid 5 parts；
Described organic acid and/or the salt of organic acid, heteroauxing, glucoseoxidase, Loprazolam, fumaric acid are at ice
Water-bath mixes, maintains the temperature at-5 DEG C of reactions and stir 5 minutes, be subsequently adding 25% peroxidase, glacial acetic acid, Fructus Forsythiae carry
Take liquid, Radix Sophorae Flavescentis extractive liquid, Caulis Spatholobi extracting solution, carbamide mixing, stand 24 hours at-5 times, obtain the mixed liquor of antibacterial；
Described by Caulis Spatholobi boiling, described amount of water is made a living 4 times of dose, and the filtrate being filtrated to get is concentrated into phase
When in 1 times of biological weight, respectively obtain the extracting solution of Caulis Spatholobi.
The described Fructus Forsythiae through preliminary cleaning treatment, carries out carbon dioxide supercritical fluid extraction, and described extraction pressure is
20Mpa, Extracting temperature is 45 DEG C, and carbon dioxide flow rate is 15L/h, and separating pressure is 15Mpa, and separation temperature is 30 DEG C, the time
Being 4 hours, products therefrom is Fructus Forsythiae extracting solution.
Described Radix Sophorae Flavescentis is carried out, and after carrying out slicing treatment, puts in the ethanol that concentration is 80% and soak 15 hours
After, pull the Radix Sophorae Flavescentis after immersion out, flame-out after ethanol solution slow fire is heated 30 minutes, obtain Radix Sophorae Flavescentis extractive liquid.
Described organic acid is selected from citric acid, lactic acid, malic acid, succinic acid, tartaric acid at least a part of which more than a kind
Acid.
The mixed liquor PH of described antibacterial is at PH5.5.
Embodiment 2
A kind of compound method of biologic formulation antibacterial mixed liquor, it is characterised in that: include by following portions by weight is former
Material is mixed:
25% peroxidase 300 parts, glacial acetic acid 3.5 parts, Fructus Forsythiae extracting solution 3 parts, Radix Sophorae Flavescentis extractive liquid 2.5 parts, Caulis Spatholobi
Extracting solution 2.5 parts, 7.5 parts of carbamide, heteroauxing 0.03 part, glucoseoxidase 150 parts, organic acid and/or the salt of organic acid
Class 100 parts, Loprazolam 15 parts, fumaric acid 12.5 parts, the barium hydroxide of 50% 2.5 parts；
Described organic acid and/or the salt of organic acid, heteroauxing, glucoseoxidase, Loprazolam, fumaric acid are at ice
Water-bath mixes, maintains the temperature at 10 DEG C of reactions and stir 10 minutes, being subsequently adding 25% peroxidase, glacial acetic acid, Fructus Forsythiae
Extracting solution, Radix Sophorae Flavescentis extractive liquid, Caulis Spatholobi extracting solution, carbamide mix, and stand 36 hours, obtain the mixing of antibacterial at 10 DEG C
Liquid；
The barium hydroxide of addition 50% regulates the pH value of the mixed liquor of this antibacterial.
Described by Caulis Spatholobi boiling, described amount of water is made a living 5 times of dose, and the filtrate being filtrated to get is concentrated into phase
When in 1.5 times of biological weight, respectively obtain the extracting solution of Caulis Spatholobi.
The described Fructus Forsythiae through preliminary cleaning treatment, carries out carbon dioxide supercritical fluid extraction, and described extraction pressure is
22.5Mpa, Extracting temperature is 47.5 DEG C, and carbon dioxide flow rate is 17.5L/h, and separating pressure is 17.5Mpa, and separation temperature is
37.5 DEG C, the time is 5 hours, and products therefrom is Fructus Forsythiae extracting solution.
Described Radix Sophorae Flavescentis is carried out, and after carrying out slicing treatment, puts into that to soak 32 in the ethanol that concentration is 88.5% little
Shi Hou, pulls the Radix Sophorae Flavescentis after immersion out, flame-out after being heated 37 minutes by ethanol solution slow fire, obtains Radix Sophorae Flavescentis extractive liquid.
Described organic acid is selected from citric acid, lactic acid, malic acid, succinic acid, tartaric acid at least a part of which more than a kind
Acid.
The mixed liquor PH of described antibacterial is at PH5.
Embodiment 3
A kind of compound method of biologic formulation antibacterial mixed liquor, it is characterised in that: include by following portions by weight is former
Material is mixed:
25% peroxidase 400 parts, glacial acetic acid 5 parts, Fructus Forsythiae extracting solution 4 parts, Radix Sophorae Flavescentis extractive liquid 3 parts, Caulis Spatholobi is extracted
Liquid 3 parts, 10 parts of carbamide, heteroauxing 0.05 part, glucoseoxidase 200 parts, the salt of organic acid and/or organic acid 150 parts,
Loprazolam 20 parts, fumaric acid 20 parts, the barium hydroxide of 50% 5 parts；
Described organic acid and/or the salt of organic acid, heteroauxing, glucoseoxidase, Loprazolam, fumaric acid are at ice
Water-bath mixes, maintains the temperature at 15 DEG C of reactions and stir 15 minutes, being subsequently adding 25% peroxidase, glacial acetic acid, Fructus Forsythiae
Extracting solution, Radix Sophorae Flavescentis extractive liquid, Caulis Spatholobi extracting solution, carbamide mix, and stand 48 hours, obtain the mixing of antibacterial at 25 DEG C
Liquid；
The barium hydroxide of addition 50% regulates the pH value of the mixed liquor of this antibacterial.
Described by Caulis Spatholobi boiling, described amount of water is made a living 6 times of dose, and the filtrate being filtrated to get is concentrated into phase
When in 2 times of biological weight, respectively obtain the extracting solution of Caulis Spatholobi.
The described Fructus Forsythiae through preliminary cleaning treatment, carries out carbon dioxide supercritical fluid extraction, and described extraction pressure is
25Mpa, Extracting temperature is 50 DEG C, and carbon dioxide flow rate is 20L/h, and separating pressure is 20Mpa, and separation temperature is 45 DEG C, the time
Being 6 hours, products therefrom is Fructus Forsythiae extracting solution.
Described Radix Sophorae Flavescentis is carried out, and after carrying out slicing treatment, puts in the ethanol that concentration is 97% and soak 48 hours
After, pull the Radix Sophorae Flavescentis after immersion out, flame-out after ethanol solution slow fire is heated 45 minutes, obtain Radix Sophorae Flavescentis extractive liquid.
Described organic acid is selected from citric acid, lactic acid, malic acid, succinic acid, tartaric acid at least a part of which more than a kind
Acid.
The mixed liquor PH of described antibacterial is at PH4.5.
Claims (6)
1. the compound method of a biologic formulation antibacterial mixed liquor, it is characterised in that: include by the raw material of following portions by weight
It is mixed:
25% peroxidase 200～400 parts, glacial acetic acid 2～5 parts, Fructus Forsythiae extracting solution 2～4 parts, Radix Sophorae Flavescentis extractive liquid 1～3 parts,
Caulis Spatholobi extracting solution 2～3 parts, carbamide 5～10 parts, heteroauxing 0.01～0.05 part, glucoseoxidase 100～200 parts, have
Machine acid and/or the salt 50 of organic acid～150 parts, Loprazolam 10～20 parts, fumaric acid 5～20 parts, the barium hydroxide 0 of 50%
～5 parts；
Described organic acid and/or the salt of organic acid, heteroauxing, glucoseoxidase, Loprazolam, fumaric acid are at ice-water bath
Middle mixing, maintains the temperature at-5～15 DEG C of reactions and stirs 5～15 minutes, being subsequently adding 25% peroxidase, glacial acetic acid, company
Stick up extracting solution, Radix Sophorae Flavescentis extractive liquid, Caulis Spatholobi extracting solution, carbamide mixing, at-5～25 DEG C, stand 24～48 hours, sterilized
The mixed liquor of agent；
The barium hydroxide solution of addition 50% regulates the pH value of the mixed liquor of this antibacterial.
The compound method of a kind of biologic formulation antibacterial mixed liquor the most according to claim 1, it is characterised in that: by Sanguis Gallus domesticus
Rattan boiling, described amount of water is made a living 4～6 times of dose, the filtrate being filtrated to get is concentrated into and is equivalent to the 1 of biological weight
～2 times, respectively obtain the extracting solution of Caulis Spatholobi.
The compound method of a kind of biologic formulation antibacterial mixed liquor the most according to claim 1, it is characterised in that: Jing Guochu
The Fructus Forsythiae of step cleaning treatment, carries out carbon dioxide supercritical fluid extraction, and described extraction pressure is 20-25Mpa, and Extracting temperature is 45-
50 DEG C, carbon dioxide flow rate is 15-20L/h, and separating pressure is 15-20Mpa, and separation temperature is 30-45 DEG C, and the time is that 4-6 is little
Time, products therefrom is Fructus Forsythiae extracting solution.
The compound method of a kind of biologic formulation antibacterial mixed liquor the most according to claim 1, it is characterised in that: Radix Sophorae Flavescentis enters
Row cleans, and after carrying out slicing treatment, puts into after soaking 15-48 hour in the ethanol that concentration is 80%-97%, pull immersion out
After Radix Sophorae Flavescentis, ethanol solution slow fire is heated after 30-45 minute flame-out, obtains Radix Sophorae Flavescentis extractive liquid.
The compound method of a kind of biologic formulation antibacterial mixed liquor the most according to claim 1, it is characterised in that have described in:
Machine acid is selected from citric acid, lactic acid, malic acid, succinic acid, the acid of tartaric acid at least a part of which more than a kind.
The compound method of a kind of biologic formulation antibacterial mixed liquor the most according to claim 1, it is characterised in that: described institute
State the mixed liquor PH of antibacterial at PH5.5～PH4.5.
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| CN201610602894.0A CN106212516A (en) | 2016-07-21 | 2016-07-21 | A kind of compound method of biologic formulation antibacterial mixed liquor |
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Citations (4)
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|---|---|---|---|---|
| CN1593158A (en) * | 2004-07-12 | 2005-03-16 | 廉云鹏 | Fruit tree bactericide and its preparation method |
| CN102199592A (en) * | 2011-04-02 | 2011-09-28 | 重庆大学 | Method for preparing mixed immobilized glucose oxidase/catalase microspheres |
| CN104365674A (en) * | 2014-09-25 | 2015-02-25 | 河北省微生物研究所 | Leather anticorrosion sterilization enzyme preparation and its preparation method |
| CN105287790A (en) * | 2015-11-25 | 2016-02-03 | 天津市中升挑战生物科技有限公司 | Paederia scandens extract and applications thereof |
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2016
- 2016-07-21 CN CN201610602894.0A patent/CN106212516A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1593158A (en) * | 2004-07-12 | 2005-03-16 | 廉云鹏 | Fruit tree bactericide and its preparation method |
| CN102199592A (en) * | 2011-04-02 | 2011-09-28 | 重庆大学 | Method for preparing mixed immobilized glucose oxidase/catalase microspheres |
| CN104365674A (en) * | 2014-09-25 | 2015-02-25 | 河北省微生物研究所 | Leather anticorrosion sterilization enzyme preparation and its preparation method |
| CN105287790A (en) * | 2015-11-25 | 2016-02-03 | 天津市中升挑战生物科技有限公司 | Paederia scandens extract and applications thereof |
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| 福州军区药物专业组: "《药物学》", 31 December 1973 * |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161214 |
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| RJ01 | Rejection of invention patent application after publication |