CN110100956A - It is a kind of with anti-oxidant and immunoloregulation function " acid+essential oil " coating type feed addictive and its preparation method and application - Google Patents
It is a kind of with anti-oxidant and immunoloregulation function " acid+essential oil " coating type feed addictive and its preparation method and application Download PDFInfo
- Publication number
- CN110100956A CN110100956A CN201910462836.6A CN201910462836A CN110100956A CN 110100956 A CN110100956 A CN 110100956A CN 201910462836 A CN201910462836 A CN 201910462836A CN 110100956 A CN110100956 A CN 110100956A
- Authority
- CN
- China
- Prior art keywords
- essential oil
- parts
- acid
- coating
- feed addictive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
Abstract
The invention discloses a kind of with anti-oxidant and immunoloregulation function " acid+essential oil " coating type feed addictive and its preparation method and application, which includes coating wall material, coating core material, plants essential oil compound, organic-acid complex, antioxidant, sodium butyrate, catalase, glucose oxidase.Plants essential oil compound, organic-acid complex, antioxidant, sodium butyrate, catalase, glucose oxidase are combined by the present invention, and by rational proportion, obtain a kind of completely new feed addictive.Feed addictive of the invention has excellent physiological function and immunomodulatory effect.It is demonstrated experimentally that novel fodder additive makes grice diarrhoea rate reduce 83% or more；The novel fodder additive can also improve the daily gain of live pig simultaneously, and reduce feedstuff-meat ratio, reduce respiratory disease, illustrate product of the present invention to pig growth is promoted, and improve efficiency of feed utilization, and enhancing immunity of organisms etc. has extraordinary help.
Description
Technical field
It is the present invention relates to a kind of animal feed additive, in particular to a kind of with anti-oxidant and immunoloregulation function
" acid+essential oil " coating type feed addictive and its preparation method and application.
Background technique
Antibiotic plays important facilitation to the development of aquaculture, but long-term abuse, abuse resistance are plain, are easy to lead
The generation for the problems such as causing medicament residue, drug resistance and intestinal bacilli illness.Countries in the world are all being reduced or are being forbidden to use at present
Antibiotic, especially because the domestic various animality horizontal safety problem incident layers caused by feed safety go out not in recent years
Thoroughly, so that vast livestock practitioner is recognized the importance of feed safety, therefore find part substitution or substitute completely anti-
The green feed additive of raw element is that herding scientific research practitioner compels the thing highly necessary done.
Plants essential oil belongs to one of plant extracts, is a kind of volatile liquid of fragranced, have food calling, growth promotion,
The effects of inhibiting bacteria and diminishing inflammation, resisting stress, disease preventing and treating, anti-feed mold, therefore the use of plants essential oil is increasingly becoming animal foodstuff
Safety in production and feed industry new growth engines.Many plants essential oils such as Herba Origani Vulgaris quintessence oil etc. is ratified by the Ministry of Agriculture, China to dynamic
It is used in object as feed addictive, with good physiological function and immunoregulation effect.Organic acid, which refers to, has acid
The organic compound of property, it can enter bacterial cell membrane by the acid ion or hydrogen ion dissociateed, increase infiltration intracellular
Pressure thoroughly reduces interior environment acid value, bacterium eubolism is made to get muddled, or even cracking is dead, to reduce harmful bacteria indirectly
Quantity.Although there is certain research to plants essential oil and organic acid at present, compatibility for plants essential oil and its with it is organic
Acid is compound on antibacterial, anti-oxidant, to improve the research that influences of the effects of growth performance less, how to be developed using related raw material more excellent
The natural green feed additive of matter has become the important content and research hotspot of feed addictive development.
Summary of the invention
In view of the deficiencies of the prior art, the object of the present invention is to provide a kind of with anti-oxidant and immunoloregulation function
" acid+essential oil " coating type feed addictive and its preparation method and application solves other synthetic drugs such as antibiotic and is added to
There are many drawbacks such as toxic side effect in feed, good result is achieved.
To achieve the goals above, the technical scheme adopted by the invention is that:
It is a kind of with anti-oxidant and immunoloregulation function " acid+essential oil " coating type feed addictive, including following weight
The raw material of part: 15 parts -20 parts of wall material of coating, 30 parts -50 parts of core material of coating, 15 parts -20 parts of plants essential oil compound, organic acid are multiple
Close 20 parts -25 parts of object, 2 parts -5 parts of antioxidant, 3 parts -5 parts of sodium butyrate, 2 parts -3 parts of catalase, glucose oxidase 1
- 2 parts of part.
Coating wall material is tristerin, and coating core material is microcrystalline cellulose.
Plants essential oil compound includes the raw material of following weight ratio: carvacrol 5-10: Thymol 16-20: rosemary essence
Oily 3-6: Forsythia suspense essential oil 2-5: Salvia japonica essential oil 12-15: cinnamon essential oil 20-25: Basil Essential 2-4: tea tree ethereal oil 8-12: betel
Essential oil 3-5: Pogostemon cablin essential 6-8: aniseed essential oil 15-18.
Organic-acid complex includes the raw material of following weight ratio: citric acid 10-30: malic acid 10-15: tartaric acid 1-3: mountain
Pears acid 15-20: fumaric acid 20-25.
Antioxidant is tert-butyl hydroquinone or dibutyl hydroxy toluene.
Betel essential oil is the preparation method comprises the following steps: the ratio co-grinding of the stem and leaf of betel by weight 1:2, crushed material will be dried
It is mixed with the ethanol solution of 70%-90% (v/v) in the ratio of solid-liquid ratio 1:5-10 (g/mL), under 200r/min stirring condition
1h is impregnated, 50 DEG C are heated to reflux 2h, collect filtrate, filter residue are repeated to extract 3 times, merging filtrate is concentrated into Rotary Evaporators
The 1/3 of original volume obtains betel essential oil.
A kind of preparation method with anti-oxidant and immunoloregulation function " acid+essential oil " coating type feed addictive, packet
Include following steps:
(1) each raw material for weighing plants essential oil compound and organic-acid complex in proportion, is sufficiently mixed, and it is mixed to form first
Close object；
(2) antioxidant, sodium butyrate, catalase, glucose oxidase, stirring are added in the mixing of the first mixture
Uniformly, the second mixture is formed；
(3) the second mixture and coating core material are sufficiently mixed absorption, are coated with for the first time through bottom spray fluidized-bed coating machine；
(4) coating wall material is atomized through spray gun after 35 DEG C of heating and meltings and sprays into fluidized bed, is coated with to first coating object
It is granulated, then obtains finished product additive after the screening of 30-60 mesh classifying screen.
A kind of application with anti-oxidant and immunoloregulation function " acid+essential oil " coating type feed addictive, feed add
Adding agent additive amount in pannage is 0.1-0.2kg/t, and additive amount is 0.1-0.15kg/t in fowl feed.
The invention has the advantages that:
1, the present invention is with carvacrol, Thymol, Rosemary Oil, Forsythia suspense essential oil, Salvia japonica essential oil, cinnamon essential oil, sieve
11 kinds of plants essential oils such as essential oil, tea tree ethereal oil, betel essential oil, Pogostemon cablin essential, aniseed essential oil are strangled as raw material, each raw material all has
Standby identical or unique function can accelerate the update speed of intestinal villus surface-ripe enterocyte such as carvacrol and Thymol
Rate, while having facilitation to the maturation of alimentary canal endodermis, stimulation enteron aisle villus develops, increases and digest and assimilate area；Cortex cinnamomi
Essential oil has the function of food calling, can inhibit bad smell in feed；Salvia japonica essential oil has the function of anti-oxidant, anti-aging；
Pogostemon cablin essential has antibacterial, anti-inflammatory, promotes the effect of cell metabolism；Basil Essential, which has, promotes digestion, prevention just
Secret effect；Tea tree ethereal oil has that good broad-spectrum sterilization is antibacterial and health-care effect, can antiviral, antibacterial, activation immune factor,
Anti parasitic, promotion wound healing etc.；Rosemary Oil primary bioactivity such as australene, nopinene, camphor and 1,8- folium eucalypti
Element has extremely strong antibiotic property and oxidation resistance, furthermore also obtains in terms of antidepression, anti-inflammatory, antitumor and improvement
To extensive use；Forsythia suspense essential oil main component is alkene terpenoid and alkyl compound, has certain antioxidant effect, together
When have different degrees of inhibitory effect to bacterium, mould and yeast, antimicrobial component has good thermal stability；Betel essential oil
Main component be chavicol, chavicol, 4- allyl pyrocatechol, carvacrol, eugenol, p-cymene, 1, 8-Cineole,
Eugenol methyl ether, carypohyllene, cadinene have wind-dispelling, eliminating dampness, desinsection, antipruritic, treatment cough due to wind-cold evil, stomachache, scabies leprosy, eczema etc.
Effect；Aniseed essential oil chemical component is mainly a variety of by anisic aldehyde, limonene, trans-anethole, chavicol methyl ether and β-carypohyllene etc.
Compound composition, has certain antibacterial and anti-oxidation characteristics.Compatibility is reasonable between each ingredient, and effect is complementary, passes through biochemical reaction
The synergistic functions such as preferable antibacterial, anti-oxidant, anti-inflammatory, raising animal immunizing power can be generated.The compounding of essential oil can be with simultaneously
Expand antimicrobial spectrum, enhances antibacterial effect, to reduce dosage (concentration).The compound additive can effectively inhibit golden yellow Portugal
Grape coccus (antibacterial circle diameter > 12mm), Escherichia coli (antibacterial circle diameter > 19.4mm), Bacillus cercus (antibacterial circle diameter
> 17.1mm), salmonella (antibacterial circle diameter > 15.5mm) etc..
2, citric acid, malic acid, tartaric acid, sorbic acid, fumaric acid that the present invention uses etc. is organic acid, antibacterial machine
Reason is mainly energy competition, permeabilization bacterial outer membrane, changes osmotic pressure, inhibits macromolecular synthesis and induction peptide expression, each
In kind antimicrobial mechanism, organic acid inhibits the effect of pathogen proliferation the most prominent in effect intracellular, and inhibits in enteron aisle effective
While harmful bacteria is proliferated, the metabolism of the growth of probiotics such as Bacillus acidi lactici is not influenced.These types of organic acid cooperates with compatibility, acidity
By force, dosage is few, and effect is fast.
3, the present invention is the compound that a variety of natural plant essential oils and organic acid are combined through scientific matching, natural plants
Essential oil bacteriostasis under low ph condition caused by organic acid is stronger, is on the one hand H+The effect of itself, pH are reduced, bacterium institute
Place's environment changes, and reduces the activity of bacterium；On the other hand, pH reduces the phenol for causing institute's band on nonpolar phenolic compound
The degree of ionization of hydroxyl becomes smaller, and hydrophobicity increases, and phenols is more soluble in the lipid phase of bacterial cell membrane and the hydrophobic region of protein, and
Protein binding capacity on cell membrane is stronger, and bacteriostasis increases, therefore the compound additive is to conventional pathogenic bacteria such as large intestine
Bacillus has good restraining and sterilizing bacteria effect, and use that is alternative or reducing antibiotic has prevention to diarrhea caused by animal bacteria
And therapeutic effect, animal immunizing power can be enhanced, promote animal digestion, improve food conversion ratio.
4, it is tert-butyl hydroquinone, dibutyl hydroxy toluene that antioxidant, which is principle active component, in the present invention.Antioxygen
Agent terminates free radical chain reactions using H ion, plays antioxidation, can plants essential oil effectively in stabilization additives, have
The components such as machine acid avoid being oxidized destruction.
5, glucose oxidase and catalase consume oxygen and generate grape by redox system in the present invention
Saccharic acid and hydrogen peroxide.Gluconic acid can reduce animal intestinal tract pH value, increase the quantity of beneficial bacterium；Hydrogen peroxide can effectively be prevented
The only growth of pathogenic entero becteria such as Escherichia coli etc., and then maintain or adjust microbial population of animal intestinal tract balance.
6, on the one hand preparation method in the present invention, coating technique can increase the raw materials such as organic acid, essential oil, antioxidant and match
On the other hand 5 combined stability enable the additive effectively by gastric acid, the slow release in animal intestinal tract, to make
The effective component for obtaining product can reach rear intestinal physiology and the immunization of animal.
7, of the invention by plants essential oil compound, organic-acid complex, antioxidant, sodium butyrate, catalase, grape
Carbohydrate oxidase is combined, and by rational proportion, obtains a kind of completely new feed addictive.Feed addictive tool of the invention
There are excellent physiological function and immunomodulatory effect.It is demonstrated experimentally that novel fodder additive makes grice diarrhoea rate reduce 83%
More than；The novel fodder additive can also improve the daily gain of live pig simultaneously, and reduce feedstuff-meat ratio, reduce respiratory disease,
Illustrate product of the present invention to pig growth is promoted, improve efficiency of feed utilization, enhancing immunity of organisms etc. has extraordinary help.
Specific embodiment
Specific embodiments of the present invention will be described in further detail with reference to embodiments.
Betel essential oil is the preparation method comprises the following steps: the ratio co-grinding of the stem and leaf of betel by weight 1:2, crushed material will be dried
It is mixed with the ethanol solution of 80% (v/v) in the ratio of solid-liquid ratio 1:10 (g/mL), impregnates 1h under 200r/min stirring condition,
50 DEG C are heated to reflux 2h, collect filtrate, filter residue are repeated to extract 3 times, merging filtrate is concentrated into original volume with Rotary Evaporators
1/3, obtain betel essential oil.
Embodiment 1
It is a kind of with anti-oxidant and immunoloregulation function " acid+essential oil " coating type feed addictive, including following weight
The raw material of part: 15 parts of wall material of coating, coating 35 parts of core material, 15 parts of plants essential oil compound, 23 parts of organic-acid complex, dibutyl
5 parts of hydroxy-methylbenzene, 3.5 parts of sodium butyrate, 2 parts of catalase, 1.5 parts of glucose oxidase.
Plants essential oil compound includes the raw material of following weight ratio: carvacrol 5: Thymol 20: Rosemary Oil (CAS:
8000-25-7) 5: Forsythia suspense essential oil (CAS:68917-49-7) 5: Salvia japonica essential oil (CAS:8022-56-8) 12: cinnamon essential oil
(CAS:8007-80-5) 22: Basil Essential (CAS:8015-73-4) 4: tea tree ethereal oil (CAS:68647-73-4) 10: betel essence
Oil 4: Pogostemon cablin essential (CAS:8014-09-3) 6: aniseed essential oil (CAS:84775-42-8) 15.
Organic-acid complex includes the raw material of following weight ratio: citric acid 20: malic acid 15: tartaric acid 3: sorbic acid 20:
Fumaric acid 20.
Preparation method, comprising the following steps:
(1) each raw material for weighing plants essential oil compound and organic-acid complex in proportion, is sufficiently mixed, and it is mixed to form first
Close object；
(2) antioxidant, sodium butyrate, catalase, glucose oxidase, stirring are added in the mixing of the first mixture
Uniformly, the second mixture is formed；
(3) by the second mixture and coating core material microcrystalline cellulose be sufficiently mixed absorption, through bottom spray fluidized-bed coating machine into
The first coating of row；
(4) coating wall material tristerin is atomized through spray gun after 35 DEG C of heating and meltings and sprays into fluidized bed, to first packet
Coating granulation is carried out by object, then obtains finished product additive after the screening of 30-60 mesh classifying screen.
Embodiment 2
It is a kind of with anti-oxidant and immunoloregulation function " acid+essential oil " coating type feed addictive, including following weight
The raw material of part: 18 parts of wall material of coating, coating 32 parts of core material, 18 parts of plants essential oil compound, 20 parts of organic-acid complex, tert-butyl
5 parts of hydroquinone, 3.5 parts of sodium butyrate, 2 parts of catalase, 1.5 parts of glucose oxidase.
Plants essential oil compound includes the raw material of following weight ratio: carvacrol 10: Thymol 18: Rosemary Oil 3.5:
Forsythia suspense essential oil 5: Salvia japonica essential oil 15: cinnamon essential oil 25: Basil Essential 2.5: tea tree ethereal oil 8: betel essential oil 3: Pogostemon cablin essential
8: aniseed essential oil 18.
Organic-acid complex includes the raw material of following weight ratio: citric acid 30: malic acid 10: tartaric acid 1: sorbic acid 16:
Fumaric acid 25.
Preparation method is the same as embodiment 1.
Embodiment 3
It is a kind of with anti-oxidant and immunoloregulation function " acid+essential oil " coating type feed addictive, including following weight
The raw material of part: 15 parts of wall material of coating, coating 39 parts of core material, 15 parts of plants essential oil compound, 20 parts of organic-acid complex, dibutyl
2 parts of hydroxy-methylbenzene, 5 parts of sodium butyrate, catalase-3 part, 1 part of glucose oxidase.
Plants essential oil compound includes the raw material of following weight ratio: carvacrol 8: Thymol 16: Rosemary Oil 6: even
Stick up essential oil 2: Salvia japonica essential oil 13: cinnamon essential oil 20: Basil Essential 3: tea tree ethereal oil 12: betel essential oil 5: Pogostemon cablin essential 7: eight
Angle essential oil 16.
Organic-acid complex includes the raw material of following weight ratio: citric acid 10: malic acid 12: tartaric acid 2: sorbic acid 15:
Fumaric acid 22.
Preparation method is the same as embodiment 1.
Compliance test result
1, bacteriostatic experiment:
Fresh bacterial strain (staphylococcus aureus, Escherichia coli, Bacillus cercus, salmonella) slant culture is taken to use
It is 1 × 10 that sterile saline, which prepares concentration,4~1 × 105The bacteria suspension of CFU/ml, sterile 100 μ l of absorption bacteria suspension is in corresponding training
Primary surface is supported, coating is uniform.Additive of the present invention product 1g is set in ± 0.1 simulated intestinal fluid of 10mLpH=6.8, magnetic agitation 4h
20 μ l drops are accurately drawn afterwards in the sterile round scraps of paperOn, it is placed with after 37 DEG C of drying in media surface, it is negative
It compares the scraps of paper and 20 μ l sterile distilled waters is added dropwise.37 DEG C of cultures 18~for 24 hours, antibacterial circle diameter is measured, indicates antibacterial work greater than 7mm
With.
The result shows that additive of the present invention can effectively inhibit pathogenic entero becteria, such as staphylococcus aureus (antibacterial circle diameter
> 12mm), Escherichia coli (antibacterial circle diameter > 19.4mm), Bacillus cercus (antibacterial circle diameter > 17.1mm), Salmonella
Bacterium (antibacterial circle diameter > 15.5mm).
2, weanling pig is tested:
This test uses 180 weanling pigs (28 ± 2d of Weaning Age, starting weight 7kg or so), is randomly divided at 3
Reason, 6 repetitions of each processing, 10 piglets of each repetition.Processing one is blank group, feeds basal diet, does not add feed and add
Add agent；It handles and adds novel fodder additive 0.1kg/t in embodiment 1 in two basal diets；It handles and is added in three basal diets
Novel fodder additive 0.1kg/t in embodiment 2.Experimental period is 30 days, and piglet uses free diet and drinking-water during test, is exempted from
Epidemic disease program and other feeding managements provide to carry out according to pig farm.
(1) influence of the novel fodder additive to weaned piglets
Average daily gain, daily ingestion amount, feedstuff-meat ratio and diarrhea rate during testing are calculated, the results are shown in Table 1.
Influence of 1 novel fodder additive of table to weaned piglets
| Project | Processing one | Processing two | Processing three |
| Starting weight (kg) | 6.96±1.05 | 6.85±0.99 | 6.87±0.62 |
| Daily gain (kg) | 0.48±0.51 | 0.59±0.63 | 0.56±0.74 |
| Daily ingestion amount (kg) | 0.71±0.45 | 0.79±0.29 | 0.76±0.31 |
| Feedstuff-meat ratio | 1.47±0.07 | 1.33±0.09 | 1.35±0.12 |
| Diarrhea rate (%) | 19.3±1.85 | 3.2±1.42 | 2.5±1.21 |
As shown in Table 1, novel fodder additive of the present invention can effective prevention of diarrhea in piglets, compared to the blank group, piglet abdomen
Rush down rate reduces 83.4% and 87.0% respectively；The novel fodder additive can also improve the daily gain of live pig simultaneously, and reduce
Feedstuff-meat ratio illustrates that product of the present invention also has extraordinary help to promotion pig growth and raising efficiency of feed utilization.
(2) influence of the novel fodder additive to piglet respiratory disease
Observe each processing group pig whether there is or not cough, fever, nasal obstruction, sneeze, air-breathing be abnormal, the hair sound of snoring, dog sitting type abdominal respiration,
Or when food, eats several mouthfuls of new line mouths and deeply breathe or do the respiratory tracts abnormal symptoms such as ventilation movement, the results are shown in Table 2.
Influence of 2 novel fodder additive of table to piglet respiratory disease
| Project | Processing one | Processing two | Processing three |
| Respiratory diseases disease incidence (%) | 7.2±3.28 | 2.6±3.53 | 1.8±2.67 |
As shown in Table 2, the respiratory tract disease incidence of three piglets of processing two and processing, which is significantly lower than, handles one (P < 0.05), says
The humoral immunity and cellular immune function of body can be enhanced in bright novel fodder additive, improves natural killer cells quantity.
(3) Serum antioxidant indices measure
After experiment, each repetition randomly selects 2 piglets, and early morning empty stomach vena cava anterior takes a blood sample 5ml in the solidifying pipe of rush,
3500r/min is centrifuged 5min after standing 30min, supernatant is taken, with kit to Serum antioxidant indices total antioxidant capacity (T-
AOC), total number born (T-SOD) activity, malonaldehyde (MDA) content are measured, and the results are shown in Table 3.
3 novel fodder additive of table is on the oxidation resistant influence of piglet serum
| Project | Processing one | Processing two | Processing three |
| T-AOC(U/ml) | 2.28±0.16 | 3.85±0.15 | 4.18±0.21 |
| T-SOD(U/ml) | 63.35±0.27 | 78.12±0.19 | 72.96±0.11 |
| MDA(mmol/ml) | 3.64±0.14 | 3.24±0.21 | 3.11±0.19 |
As shown in Table 3, two, three groups of soluble transferring receptors, total number born activity of processing group are significantly higher than
Processing group one, serum malondialdehyde content are substantially less than processing group one.It can be seen that adding compound additive in diet can improve
The oxidation resistance of piglet.
(4) influence of the novel fodder additive to intestine of young pigs microbial flora
When testing the 30th day, collection fresh excreta, which is placed in ice chest, to be saved, and after transporting laboratory back, is weighed under aseptic condition
1.0g content is added sterile saline 9ml, rear doubling dilution fullys shake, is respectively coated Mai Kang in sterile test tube
Then the triumphant aerobic culture of agar medium carries out bacterium colony counting, the results are shown in Table 4 for 24 hours with MRS agar medium Anaerobic culturel 48h.
Influence of 4 novel fodder additive of table to intestine of young pigs flora
| Project | Processing one | Processing two | Processing three |
| Bacillus acidi lactici (cfu/g) | 7.58±0.08 | 8.39±0.02 | 8.28±0.03 |
| Escherichia coli (cfu/g) | 6.88±0.07 | 5.82±0.09 | 5.65±0.07 |
As shown in Table 4, processing two, three is compared with processing one, beneficial bacterium in intestine of young pigs after feeding novel fodder additive
The quantity of (Bacillus acidi lactici) significantly improves, and the quantity of harmful bacteria (Escherichia coli) is greatly reduced.
3, broiler chicken experimental verification:
5 age in days Huang plumage meat cock 180 of physical health are chosen, at random point 3 groups, each group of 5 repetitions, each repetition
12.Processing one is blank group, feeds basal diet, does not add feed addictive；Handle addition embodiment 1 in two basal diets
Middle novel fodder additive 0.15kg/t；It handles and adds novel fodder additive 0.15kg/t in embodiment 2 in three basal diets.
Test chicken daily hand fed 2 times is freely eaten, drinking-water.Immune, disinfection is carried out by broiler chicken conventional program.In test the 35th
It when, it is every repeat to choose 3 broiler chicken close to average weight take a blood sample and butcher sampling.
(1) influence of the novel fodder additive to meat chicken growth performance
Every chicken birth weight is recorded when on-test, then at the 35th day 12 by only weighing, weigh preceding empty stomach 12h,
Calculate each stage average daily gain.As unit of repetition, precise simultaneously records each duplicate day inventory, clout amount and loss
Amount calculates each stage average daily gain, and then calculates each stage feed-weight ratio according to average daily gain and average daily gain.Together
When, dead chicken number is recorded daily, is calculated each stage survival rate, be the results are shown in Table 5.
Influence of 5 novel fodder additive of table to meat chicken growth performance
| Project | Processing one | Processing two | Processing three |
| Average daily gain (g/ days) | 52.72±3.24 | 53.41±2.15 | 52.14±1.75 |
| Average daily gain (g/ days) | 33.15±0.24 | 35.24±0.51 | 34.71±0.33 |
| Feed-weight ratio | 1.59±0.11 | 1.51±0.09 | 1.50±0.13 |
| Survival rate (%) | 91.25±0.11 | 94.52±0.15 | 95.73±0.08 |
As shown in Table 5, novel fodder additive can increase average daily gain in same feed intake, reduce material weight
Than improving survival rate, significantly improving meat chicken growth performance.
(2) measurement of Biochemical Indices In Serum
At test chicken the 35th day 12 by only weigh after, with vacuum blood collection tube carries out wing venous blood sampling 5ml, inclination standing,
After serum exudation, 3000r/min is centrifuged 15min, takes supernatant, is sub-packed in EP pipe, is placed in -80 DEG C and freezes.Using reagent
Box testing index Biochemical Indices In Serum, including total serum protein, albumin, uric acid content, are respectively adopted Coomassie Brilliant Blue, bromine
The green method of cresols and colorimetric method are measured, and the results are shown in Table 6.
Influence of 6 novel fodder additive of table to serum of broilers biochemical indicator
| Project | Processing one | Processing two | Processing three |
| Albumin (g/L) | 22.67±0.78 | 26.72±1.02 | 28.14±0.85 |
| Total protein (g/L) | 25.21±2.15 | 28.45±1.65 | 30.07±1.03 |
| Uric acid (μm ol/L) | 268.12±18.24 | 254.77±10.73 | 241.67±14.52 |
As shown in Table 6, novel fodder additive can significantly improve serum of broilers biochemical indicator, improve albumin and total protein
Level reduces uric acid content, improves body to the utilization rate of protein, promotes protein in the intracorporal deposition of machine, to promote
The growth of broiler chicken.
Claims (8)
1. a kind of with anti-oxidant and immunoloregulation function " acid+essential oil " coating type feed addictive, which is characterized in that including
The raw material of following parts by weight: coating 15 parts -20 parts of wall material, coating 30 parts -50 parts of core material, 15 parts -20 parts of plants essential oil compound,
20 parts -25 parts of organic-acid complex, 2 parts -5 parts of antioxidant, 3 parts -5 parts of sodium butyrate, 2 parts -3 parts of catalase, glucose
1 part -2 parts of oxidizing ferment.
2. feed addictive according to claim 1, which is characterized in that the coating wall material is tristerin,
Coating core material is microcrystalline cellulose.
3. feed addictive according to claim 1, which is characterized in that the plants essential oil compound includes following heavy
The raw material of amount ratio: carvacrol 5-10: Thymol 16-20: Rosemary Oil 3-6: Forsythia suspense essential oil 2-5: Salvia japonica essential oil 12-
15: cinnamon essential oil 20-25: Basil Essential 2-4: tea tree ethereal oil 8-12: betel essential oil 3-5: Pogostemon cablin essential 6-8: aniseed essential oil
15-18。
4. feed addictive according to claim 1, which is characterized in that the organic-acid complex includes following weight
The raw material of ratio: citric acid 10-30: malic acid 10-15: tartaric acid 1-3: sorbic acid 15-20: fumaric acid 20-25.
5. feed addictive according to claim 1, which is characterized in that the antioxidant is tert-butyl hydroquinone
Or dibutyl hydroxy toluene.
6. plants essential oil compound according to claim 3, which is characterized in that the betel essential oil the preparation method comprises the following steps:
Ratio co-grinding by the stem and leaf of dry betel by weight 1:2, the ethanol solution of crushed material and 70%-90% (v/v)
It is mixed in the ratio of solid-liquid ratio 1:5-10 (g/mL), 1h is impregnated under 200r/min stirring condition, 50 DEG C are heated to reflux 2h, collect
Filter residue is repeated to extract 3 times by filtrate, and merging filtrate is concentrated into the 1/3 of original volume with Rotary Evaporators, obtains betel essential oil.
7. a kind of preparation method of the feed addictive as described in claim any one of 1-6, which is characterized in that including following step
It is rapid:
(1) each raw material for weighing plants essential oil compound and organic-acid complex in proportion, is sufficiently mixed, and forms the first mixing
Object；
(2) antioxidant, sodium butyrate, catalase, glucose oxidase are added in the mixing of the first mixture, stirring is equal
It is even, form the second mixture；
(3) the second mixture and coating core material are sufficiently mixed absorption, are coated with for the first time through bottom spray fluidized-bed coating machine；
(4) coating wall material is atomized through spray gun after 35 DEG C of heating and meltings and sprays into fluidized bed, carries out coating to first coating object and makes
Grain, then finished product additive is obtained after the screening of 30-60 mesh classifying screen.
8. a kind of application of the feed addictive as described in claim any one of 1-6, which is characterized in that feed addictive is raised in pig
Additive amount is 0.1-0.2kg/t in material, and additive amount is 0.1-0.15kg/t in fowl feed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910462836.6A CN110100956A (en) | 2019-05-30 | 2019-05-30 | It is a kind of with anti-oxidant and immunoloregulation function " acid+essential oil " coating type feed addictive and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910462836.6A CN110100956A (en) | 2019-05-30 | 2019-05-30 | It is a kind of with anti-oxidant and immunoloregulation function " acid+essential oil " coating type feed addictive and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110100956A true CN110100956A (en) | 2019-08-09 |
Family
ID=67493018
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910462836.6A Pending CN110100956A (en) | 2019-05-30 | 2019-05-30 | It is a kind of with anti-oxidant and immunoloregulation function " acid+essential oil " coating type feed addictive and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110100956A (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110367390A (en) * | 2019-08-19 | 2019-10-25 | 东莞正大康地饲料有限公司 | A kind of nonreactive piggy premixed feed of high-efficiency environment friendly |
| CN110771729A (en) * | 2019-11-01 | 2020-02-11 | 广西壮族自治区林业科学研究院 | Feed additive containing plant essential oil and preparation method thereof |
| CN110771728A (en) * | 2019-11-01 | 2020-02-11 | 广西壮族自治区林业科学研究院 | Plant essential oil feed additive for improving immunity of livestock and preparation method thereof |
| CN110771730A (en) * | 2019-11-01 | 2020-02-11 | 广西壮族自治区林业科学研究院 | Livestock feed containing plant essential oil and preparation method thereof |
| CN111631310A (en) * | 2020-07-13 | 2020-09-08 | 四川大想生物科技有限公司 | Anti-substitution additive for animal feed and preparation method thereof |
| CN112674219A (en) * | 2020-12-21 | 2021-04-20 | 湖北浩华生物技术有限公司 | Feed additive, preparation method thereof and animal feed |
| CN113349304A (en) * | 2021-06-21 | 2021-09-07 | 重庆农笑农业发展有限公司 | Traditional Chinese medicine pig feed additive, preparation method and pig feed thereof |
| CN113412884A (en) * | 2020-12-31 | 2021-09-21 | 江苏翼邦生物技术有限公司 | Pet flavoring agent with antioxidation effect and preparation method thereof |
| CN113892561A (en) * | 2021-10-27 | 2022-01-07 | 潍坊加易加生物科技有限公司 | Slow-release feed additive for pigs and poultry and preparation method and application thereof |
| CN114041542A (en) * | 2021-12-08 | 2022-02-15 | 成都大帝汉克生物科技有限公司 | Feed additive for promoting piglet gastrointestinal health and preparation method thereof |
| CN115067434A (en) * | 2022-06-20 | 2022-09-20 | 北京都润科技有限公司 | Feed additive for broiler chickens and preparation method and application thereof |
| CN116098240A (en) * | 2022-12-02 | 2023-05-12 | 安佑生物科技集团股份有限公司 | Feed additive for improving anti-cyst membrane virus capability of pigs and preparation method thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103082093A (en) * | 2013-01-31 | 2013-05-08 | 武汉工业学院 | Coated natural plant essential oil feed additive and application thereof |
| US20140037698A1 (en) * | 2011-01-21 | 2014-02-06 | Norel, S.A | Additives for animal food |
| CN105192312A (en) * | 2015-09-18 | 2015-12-30 | 河北农业大学 | Pig feed additive and preparation method thereof |
| CN106387439A (en) * | 2016-10-28 | 2017-02-15 | 上海申亚动物保健品阜阳有限公司 | Anti-diarrhea feed additive for piglets |
| CN107095072A (en) * | 2017-04-26 | 2017-08-29 | 盐城惠民饲料科技有限公司 | A kind of nonreactive type laying hens in brooding period ecological nutrition compound feed and preparation method thereof |
| CN107156455A (en) * | 2017-07-19 | 2017-09-15 | 浙江万方生物科技有限公司 | A kind of acid plus essential oil compound formulation and preparation method thereof |
| CN108651710A (en) * | 2018-06-28 | 2018-10-16 | 济南牧德生物科技有限公司 | A kind of Efficient antibacterial composite essential oil and preparation method thereof |
| CN109077180A (en) * | 2018-07-31 | 2018-12-25 | 广东蔚莱生物科技有限公司 | A kind of composite plant essential oil feed addictive and the preparation method and application thereof |
-
2019
- 2019-05-30 CN CN201910462836.6A patent/CN110100956A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140037698A1 (en) * | 2011-01-21 | 2014-02-06 | Norel, S.A | Additives for animal food |
| CN103082093A (en) * | 2013-01-31 | 2013-05-08 | 武汉工业学院 | Coated natural plant essential oil feed additive and application thereof |
| CN105192312A (en) * | 2015-09-18 | 2015-12-30 | 河北农业大学 | Pig feed additive and preparation method thereof |
| CN106387439A (en) * | 2016-10-28 | 2017-02-15 | 上海申亚动物保健品阜阳有限公司 | Anti-diarrhea feed additive for piglets |
| CN107095072A (en) * | 2017-04-26 | 2017-08-29 | 盐城惠民饲料科技有限公司 | A kind of nonreactive type laying hens in brooding period ecological nutrition compound feed and preparation method thereof |
| CN107156455A (en) * | 2017-07-19 | 2017-09-15 | 浙江万方生物科技有限公司 | A kind of acid plus essential oil compound formulation and preparation method thereof |
| CN108651710A (en) * | 2018-06-28 | 2018-10-16 | 济南牧德生物科技有限公司 | A kind of Efficient antibacterial composite essential oil and preparation method thereof |
| CN109077180A (en) * | 2018-07-31 | 2018-12-25 | 广东蔚莱生物科技有限公司 | A kind of composite plant essential oil feed addictive and the preparation method and application thereof |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110367390A (en) * | 2019-08-19 | 2019-10-25 | 东莞正大康地饲料有限公司 | A kind of nonreactive piggy premixed feed of high-efficiency environment friendly |
| CN110771729A (en) * | 2019-11-01 | 2020-02-11 | 广西壮族自治区林业科学研究院 | Feed additive containing plant essential oil and preparation method thereof |
| CN110771728A (en) * | 2019-11-01 | 2020-02-11 | 广西壮族自治区林业科学研究院 | Plant essential oil feed additive for improving immunity of livestock and preparation method thereof |
| CN110771730A (en) * | 2019-11-01 | 2020-02-11 | 广西壮族自治区林业科学研究院 | Livestock feed containing plant essential oil and preparation method thereof |
| CN111631310A (en) * | 2020-07-13 | 2020-09-08 | 四川大想生物科技有限公司 | Anti-substitution additive for animal feed and preparation method thereof |
| CN112674219A (en) * | 2020-12-21 | 2021-04-20 | 湖北浩华生物技术有限公司 | Feed additive, preparation method thereof and animal feed |
| CN113412884A (en) * | 2020-12-31 | 2021-09-21 | 江苏翼邦生物技术有限公司 | Pet flavoring agent with antioxidation effect and preparation method thereof |
| CN113349304A (en) * | 2021-06-21 | 2021-09-07 | 重庆农笑农业发展有限公司 | Traditional Chinese medicine pig feed additive, preparation method and pig feed thereof |
| CN113892561A (en) * | 2021-10-27 | 2022-01-07 | 潍坊加易加生物科技有限公司 | Slow-release feed additive for pigs and poultry and preparation method and application thereof |
| CN114041542A (en) * | 2021-12-08 | 2022-02-15 | 成都大帝汉克生物科技有限公司 | Feed additive for promoting piglet gastrointestinal health and preparation method thereof |
| CN115067434A (en) * | 2022-06-20 | 2022-09-20 | 北京都润科技有限公司 | Feed additive for broiler chickens and preparation method and application thereof |
| CN116098240A (en) * | 2022-12-02 | 2023-05-12 | 安佑生物科技集团股份有限公司 | Feed additive for improving anti-cyst membrane virus capability of pigs and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110100956A (en) | It is a kind of with anti-oxidant and immunoloregulation function " acid+essential oil " coating type feed addictive and its preparation method and application | |
| CN103355545B (en) | Fermentation type botanical weaned piglet feed additive | |
| CN103125779B (en) | A kind of broiler chicken biological Chinese herbal medicine feed addictive and preparation method thereof | |
| CN101664421B (en) | Traditional Chinese biological preparation for preventing and treating livestock diarrhea and preparation method and feedstuff thereof | |
| CN101912042B (en) | Pure natural plant feed additive for preventing and controlling diarrhea and application | |
| CN108813289A (en) | A kind of preparation and the preparation method and application thereof of high-performance bio degradation mycotoxin | |
| CN110100965A (en) | A kind of derma type essential oil organic acid composite feed additive and its preparation method and application | |
| Lu | Nutritionally related strategies for organic goat production | |
| CN102697888A (en) | Traditional Chinese medicine fermented preparation for preventing and treating piglet diarrhea and preparation method thereof | |
| CN113812528A (en) | Plant extract additive for improving intestinal health of livestock and poultry | |
| CN105287790B (en) | A kind of fevervine extract and its application | |
| CN103719623A (en) | Traditional Chinese medicine feed additive capable of preventing or relieving stress syndrome of weaned pigs and preparation method of feed additive | |
| Guo et al. | Effects of the stems and leaves of Astragalus membranaceus on growth performance, immunological parameters, antioxidant status, and intestinal bacteria of quail | |
| CN109601734A (en) | Feed addictive containing procyanidine and dietary fiber and preparation method thereof and nonreactive pig starter feed product | |
| CN111011602A (en) | Functional feed additive for relieving oxidative stress of livestock and poultry and improving intestinal health and preparation method thereof | |
| CN114568602B (en) | Traditional Chinese medicine compound for improving growth performance and organism immunity of broiler chickens and preparation method and application thereof | |
| CN112136965A (en) | Immunity-enhancing and growth-promoting fermented Chinese herbal medicine feed additive and preparation method thereof | |
| KR100672657B1 (en) | Assorted feedcontained fibroid material and its preparation method | |
| Shi et al. | Dietary yeast extract complex supplementation increases growth performance and nutrient digestibility of weaning pigs | |
| CN106266101A (en) | A kind of veterinary Chinese medicinal composition and its production and use | |
| KR101197682B1 (en) | Complex Feed Supplement and method for making Fermented Green Tea Probiotics | |
| CN115088785A (en) | Preparation method and application of compound fermented traditional Chinese medicine feed additive for preventing and treating aeromonas hydrophila | |
| CN101185482A (en) | Green environmental protection deer feed additive and preparation method thereof | |
| CN101053369B (en) | Feed additive for fowls and domestic animals and preparation method thereof | |
| CN104171540B (en) | A kind of weanling pig anti-diarrhea growth-promoting changqiang immunity compound Chinese herbal medicinal feed additive and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190809 |