CN105287790B - A kind of fevervine extract and its application - Google Patents
A kind of fevervine extract and its application Download PDFInfo
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Abstract
The present invention provides a kind of fevervine extract and its applications, the fevervine extract has significant application value in terms of the enterogastric diseases such as treatment having indigestion, diarrhea, indigestion, herbal mixture is formed with rhizoma zingiberis, Radix Aconiti Lateralis Preparata, rhizoma alismatis and glucose oxidase, it is being applied to inhibit and kill pathogenic microorganism, the enterogastric diseases such as having indigestion, indigestion and diarrhea are treated, promote growth of animal, improve food conversion ratio etc. has good market prospects.
Description
Technical field
The present invention relates to field of veterinary, especially a kind of fevervine extract and its application.
Background technique
With the development of intensive animal husbandry, the disease of domestic animal also becomes increasingly complex, veterinary drug and antibiotic feed additive
Usage amount also increasingly increase.The stable antibiotic of properties causes the medicament residue in environment after being excreted in environment.This
A little remaining drugs can directly be accumulated in human body by animal products or pass through Environment release into other plants, and finally with each
Kind approach comes together in human body, leads to the drug resistance variation of the chronic toxic effect and internal normal flora of human body.Natural plant
Object unique structure is not likely to produce drug resistance, can voluntarily degrade in the environment, does not cause the irreversible breaking to environment, to people and animals
It is relatively less toxic, meet requirement of the green ecological animal husbandry to drug, is the important development direction of the following veterinary drug.
Fevervine (Paederia scandens) is madder wort fevervine herb also known as Paederia scandens and smelly rattan, mainly
The areas such as China's southeastern coast and the Yangtze river basin are distributed in, are traditional Chinese medicines.The main component of fevervine includes cyclenes ether
Terpene methods of glycosides, triterpenes components, steroidal and its methods of glycosides, Phenylpropanoid Glycosides class, flavone compound, volatile oil component and other
Ingredient.Modern pharmacology research proves that fevervine has a variety of effects such as significant anti-inflammatory, analgesia, calm and reduction uric acid.Chicken
A kind of ingredient that arrow rattan is chiefly used in feed addictive in veterinary field at present uses.
Summary of the invention
Technical problem to be solved by the present invention lies in provide a kind of fevervine extract.
Another technical problem to be solved by this invention is to provide the application of above-mentioned fevervine extract.
In order to solve the above technical problems, the technical scheme is that
A kind of fevervine extract, the iridoid glycosides containing 60-99% are prepared by the following method to obtain:
(1) fevervine medicinal material is taken, 8-12 times is added and measures 75% (v/v) alcohol reflux extraction 1-3 hours, filter extracting solution,
The dregs of a decoction repeat said extracted process 2-3 times, combined extract, are concentrated under reduced pressure, until the distillation of 2-6 times of medicinal material amount is added without alcohol taste
Water makes to dissolve, and centrifugation obtains supernatant；
(2) D101 macroporous absorbent resin on supernatant, medicinal material and amount of resin ratio are that every 1kg medicinal material needs 200-400ml through nothing
D101 macroreticular resin after water-ethanol swelling pretreatment, it is 1: 8-12 that resin column diameter height, which compares, and loading flow velocity is 1.0-2.0BV/h,
Elution flow rate is 4-6BV/h；
(3) it after loading, first with distillation water elution resin column 2BV, discards and flows through liquid and water elution, then with 20-85% (v/
V) ethanol-eluting resin column 6BV, collects the eluent of ethyl alcohol, and 60 DEG C of reduced pressures are dry to get fevervine extract.
Preferably, above-mentioned fevervine extract, is prepared by the following method to obtain:
(1) fevervine medicinal material is taken, 10 times of 75% (v/v) alcohol refluxs of amount are added and extract 2 hours, filter extracting solution, the dregs of a decoction
It repeats said extracted process 3 times, combined extract, 60 DEG C of reduced pressures, until the distilled water that 5 times of medicinal material amounts are added makes without alcohol taste
Dissolution, centrifugation, obtains supernatant；
(2) D101 macroporous absorbent resin on supernatant, medicinal material and amount of resin ratio are that every 1kg medicinal material needs 300ml through anhydrous second
D101 macroreticular resin after alcohol swelling pretreatment, it is 1: 8 that resin column diameter height, which compares, and loading flow velocity is 2.0BV/h, and elution flow rate is
6BV/h；
(3) it after loading, first with distillation water elution resin column 2BV, discards and flows through liquid and water elution, then with 30% (v/v)
Ethanol-eluting resin column 6BV, collects the eluent of ethyl alcohol, and 60 DEG C of reduced pressures are dry to get fevervine extract.
Above-mentioned fevervine extract is preparing the application in the drug for treating gastrointestinal tract of livestock and fowls disease.
It is a kind of for treating the composition of gastrointestinal tract of livestock and fowls disease, by above-mentioned fevervine extract, rhizoma zingiberis, Radix Aconiti Lateralis Preparata, pool
It rushes down and is formed with glucose oxidase, 5-10 parts of fevervine extract, 3-10 parts of rhizoma zingiberis, Radix Aconiti Lateralis Preparata 3-15 based on its parts by weight
Part, 5-10 parts of rhizoma alismatis, 3-10 parts of glucose oxidase.
Preferably, above-mentioned for treating the composition of gastrointestinal tract of livestock and fowls disease, the fevervine extract 6 based on its parts by weight
Part, 5 parts of rhizoma zingiberis, 10 parts of Radix Aconiti Lateralis Preparata, 7 parts of rhizoma alismatis, 5 parts of glucose oxidase.
The preparation method of the above-mentioned composition for being used to treat gastrointestinal tract of livestock and fowls disease, specific preparation process is as follows:
(1) fevervine extract, rhizoma zingiberis, Radix Aconiti Lateralis Preparata and rhizoma alismatis is weighed by prescription amount to be sterilized, be ground into fine powder and mistake
Sieve；
(2) add the glucose oxidase of prescription amount, be uniformly mixed to get.
Above-mentioned composition in actual production step (2) mixing after component also need that suitable pharmaceutical adjunct, the system is added
Agent auxiliary material is including but not limited to following several: starch, sucrose, DEXTROSE ANHYDROUS, auxiliary whitening, dextrin, lactose, superfine silica gel powder.
Another preparation method of the above-mentioned composition for being used to treat gastrointestinal tract of livestock and fowls disease, specific preparation process is as follows:
(1) fevervine extract, rhizoma zingiberis, Radix Aconiti Lateralis Preparata and rhizoma alismatis are weighed by prescription amount to be sterilized, crush, mix, is obtained
Bulk pharmaceutical chemicals mixed powder；
(2) by bulk pharmaceutical chemicals mixed powder, add 8-12 times to measure water and impregnate 30-60 minutes, after decocting 60-90 minutes, be obtained by filtration
Once decoct medical fluid and the dregs of a decoction；The dregs of a decoction that will be obtained, again plus suitable decocting is endured, and is obtained by filtration and secondary is decocted medical fluid；It repeats
Above-mentioned steps are decocted medical fluid three times, gained medical fluid will be mixed three times, being concentrated under reduced pressure into concentrate relative density is 1.10-
1.15；
(3) after concentrate lets cool, filters, the glucose oxidase of prescription amount is added, is uniformly mixed to obtain the final product.
Above-mentioned composition also needs that suitable sodium benzoate is added in actual production.
The beneficial effects of the present invention are:
Above-mentioned fevervine extract has important application in terms of the enterogastric diseases such as treatment having indigestion, diarrhea, indigestion
Value is especially significant to fungistatic effects such as Pasteurella, salmonella, staphylococcus aureus and Fu Shi shigella dysenteriaes；Pass through
Herbal mixture is formed with other drugs or corresponding auxiliary material is added to can be made into pre-mixing agent or mixture etc., and herbal mixture pre-mixing agent can lead to
The form administration of mixed feeding is crossed, mixture can be administered by way of drinking-water.It is being applied to inhibit and kill pathogenic microorganism, treatment product
The enterogastric diseases such as food, indigestion and diarrhea, promote growth of animal, improve food conversion ratio etc. have good market
Prospect.
When especially carrying out prescription with specific Chinese medicine, each component mutually acts synergistically, and gained is for treating gastrointestinal tract of livestock and fowls disease
The composition of disease can effectively treat the enterogastric diseases such as having indigestion, diarrhea, indigestion, guarantee the normal of animal intestines and stomach function,
The use of antibiotic is reduced, to guarantee that animal health is grown up.
Specific embodiment
Technical solution of the present invention is further described combined with specific embodiments below.
Embodiment 1
A kind of fevervine extract is prepared by the following method to obtain containing 98.3% iridoid glycosides:
(1) fevervine medicinal material is taken, 10 times of 75% (v/v) alcohol refluxs of amount are added and extract 2 hours, filter extracting solution, the dregs of a decoction
It repeats said extracted process 3 times, combined extract, 60 DEG C of reduced pressures, until the distilled water that 5 times of medicinal material amounts are added makes without alcohol taste
Dissolution, centrifugation, obtains supernatant；
(2) D101 macroporous absorbent resin on supernatant, medicinal material and amount of resin ratio are that every 1kg medicinal material needs 300ml through anhydrous second
D101 macroreticular resin after alcohol swelling pretreatment, it is 1: 8 that resin column diameter height, which compares, and loading flow velocity is 2.0BV/h, and elution flow rate is
6BV/h；
(3) it after loading, first with distillation water elution resin column 2BV, discards and flows through liquid and water elution, then with 30% (v/v)
Ethanol-eluting resin column 6BV, collects the eluent of ethyl alcohol, and 60 DEG C of reduced pressures are dry to get fevervine extract.
Embodiment 2
A kind of fevervine extract is prepared by the following method to obtain containing 89.2% iridoid glycosides:
(1) fevervine medicinal material is taken, 12 times of 75% (v/v) alcohol refluxs of amount are added and extract 3 hours, filter extracting solution, the dregs of a decoction
It repeats said extracted process 2 times, combined extract, is concentrated under reduced pressure, until the distilled water that 2 times of medicinal material amounts are added makes to dissolve without alcohol taste,
Centrifugation, obtains supernatant；
(2) D101 macroporous absorbent resin on supernatant, medicinal material and amount of resin ratio are that every 1kg medicinal material needs 400ml through anhydrous second
D101 macroreticular resin after alcohol swelling pretreatment, it is 1: 10 that resin column diameter height, which compares, and loading flow velocity is 1.5BV/h, and elution flow rate is
5BV/h；
(3) it after loading, first with distillation water elution resin column 2BV, discards and flows through liquid and water elution, then with 20% (v/v)
Ethanol-eluting resin column 6BV, collects the eluent of ethyl alcohol, and 60 DEG C of reduced pressures are dry to get fevervine extract.
Embodiment 3
A kind of fevervine extract is prepared by the following method to obtain containing 76.9% iridoid glycosides:
(1) fevervine medicinal material is taken, 8 times of 75% (v/v) alcohol refluxs of amount are added and extract 1 hour, filter extracting solution, dregs of a decoction weight
Multiple said extracted process 3 times, combined extract is concentrated under reduced pressure, until the distilled water that 6 times of medicinal material amounts are added makes to dissolve without alcohol taste, from
The heart obtains supernatant；
(2) D101 macroporous absorbent resin on supernatant, medicinal material and amount of resin ratio are that every 1kg medicinal material needs 200ml through anhydrous second
D101 macroreticular resin after alcohol swelling pretreatment, it is 1: 12 that resin column diameter height, which compares, and loading flow velocity is 1.0BV/h, and elution flow rate is
4BV/h；
(3) it after loading, first with distillation water elution resin column 2BV, discards and flows through liquid and water elution, then with 85% (v/v)
Ethanol-eluting resin column 6BV, collects the eluent of ethyl alcohol, and 60 DEG C of reduced pressures are dry to get fevervine extract.
Embodiment 4
It is a kind of for treating the composition of gastrointestinal tract of livestock and fowls disease, it is fevervine extract, rhizoma zingiberis as described in embodiment 1, black
Monkshood, rhizoma alismatis and glucose oxidase composition, wherein fevervine extract 6kg, rhizoma zingiberis 5kg, Radix Aconiti Lateralis Preparata 10kg, rhizoma alismatis 7kg,
Glucose oxidase 5kg.
The preparation method of the above-mentioned composition for being used to treat gastrointestinal tract of livestock and fowls disease, specific preparation process is as follows:
(1) fevervine extract, rhizoma zingiberis, Radix Aconiti Lateralis Preparata and rhizoma alismatis are weighed by prescription amount to be sterilized, crush, mix, is obtained
Bulk pharmaceutical chemicals mixed powder；
(2) by bulk pharmaceutical chemicals mixed powder, add 10 times of amount water to impregnate 50 minutes, after decocting 80 minutes, be obtained by filtration and once decoct
Medical fluid and the dregs of a decoction；The dregs of a decoction that will be obtained, again plus suitable decocting is endured, and is obtained by filtration and secondary is decocted medical fluid；It repeats the above steps,
Medical fluid is decocted three times, gained medical fluid will be mixed three times, being concentrated under reduced pressure into concentrate relative density is 1.13；
(3) after concentrate lets cool, filters, the glucose oxidase of prescription amount is added, is uniformly mixed to obtain the final product.
Embodiment 5
It is a kind of for treating the composition of gastrointestinal tract of livestock and fowls disease, it is fevervine extract, rhizoma zingiberis as described in embodiment 1, black
Monkshood, rhizoma alismatis and glucose oxidase composition, wherein fevervine extract 5kg, rhizoma zingiberis 10kg, Radix Aconiti Lateralis Preparata 3kg, rhizoma alismatis 10kg,
Glucose oxidase 3kg.
The preparation method of the above-mentioned composition for being used to treat gastrointestinal tract of livestock and fowls disease, specific preparation process is as follows:
(1) fevervine extract, rhizoma zingiberis, Radix Aconiti Lateralis Preparata and rhizoma alismatis is weighed by prescription amount to be sterilized, be ground into fine powder and mistake
Sieve；
(2) add the glucose oxidase of prescription amount, be uniformly mixed to get.
Embodiment 6
It is a kind of for treating the composition of gastrointestinal tract of livestock and fowls disease, it is fevervine extract, rhizoma zingiberis as described in embodiment 1, black
Monkshood, rhizoma alismatis and glucose oxidase composition, wherein fevervine extract 10kg, rhizoma zingiberis 3kg, Radix Aconiti Lateralis Preparata 15kg, rhizoma alismatis 5kg,
Glucose oxidase 10kg.
The preparation method of the above-mentioned composition for being used to treat gastrointestinal tract of livestock and fowls disease, specific preparation process is as follows:
(1) fevervine extract, rhizoma zingiberis, Radix Aconiti Lateralis Preparata and rhizoma alismatis are weighed by prescription amount to be sterilized, crush, mix, is obtained
Bulk pharmaceutical chemicals mixed powder；
(2) by bulk pharmaceutical chemicals mixed powder, add 12 times of amount water to impregnate 30 minutes, after decocting 90 minutes, be obtained by filtration and once decoct
Medical fluid and the dregs of a decoction；The dregs of a decoction that will be obtained, again plus suitable decocting is endured, and is obtained by filtration and secondary is decocted medical fluid；It repeats the above steps,
Medical fluid is decocted three times, gained medical fluid will be mixed three times, being concentrated under reduced pressure into concentrate relative density is 1.15；
(3) after concentrate lets cool, filters, the glucose oxidase of prescription amount is added, is uniformly mixed to obtain the final product.
Embodiment 7
It is a kind of for treating the composition of gastrointestinal tract of livestock and fowls disease, it is fevervine extract, rhizoma zingiberis as described in embodiment 1, black
Monkshood, rhizoma alismatis and glucose oxidase composition, wherein fevervine extract 8kg, rhizoma zingiberis 4kg, Radix Aconiti Lateralis Preparata 4kg, rhizoma alismatis 6kg, Portugal
Grape carbohydrate oxidase 7kg.
The preparation method of the above-mentioned composition for being used to treat gastrointestinal tract of livestock and fowls disease, specific preparation process is as follows:
(1) fevervine extract, rhizoma zingiberis, Radix Aconiti Lateralis Preparata and rhizoma alismatis are weighed by prescription amount to be sterilized, crush, mix, is obtained
Bulk pharmaceutical chemicals mixed powder；
(2) by bulk pharmaceutical chemicals mixed powder, add 8 times of amount water to impregnate 60 minutes, decoct after sixty minutes, be obtained by filtration and once decoct medicine
Liquid and the dregs of a decoction；The dregs of a decoction that will be obtained, again plus suitable decocting is endured, and is obtained by filtration and secondary is decocted medical fluid；It repeats the above steps, obtains
To medical fluid is decocted three times, gained medical fluid will mix three times, being concentrated under reduced pressure into concentrate relative density is 1.10；
(3) after concentrate lets cool, filters, the glucose oxidase of prescription amount is added, is uniformly mixed to obtain the final product.
Embodiment 8
It is a kind of for treating the composition of gastrointestinal tract of livestock and fowls disease, it is fevervine extract, rhizoma zingiberis as described in embodiment 1, black
Monkshood, rhizoma alismatis and glucose oxidase composition, wherein fevervine extract 7kg, rhizoma zingiberis 9kg, Radix Aconiti Lateralis Preparata 8kg, rhizoma alismatis 6kg, Portugal
Grape carbohydrate oxidase 8kg.
The preparation method of the above-mentioned composition for being used to treat gastrointestinal tract of livestock and fowls disease, specific preparation process is as follows:
(1) fevervine extract, rhizoma zingiberis, Radix Aconiti Lateralis Preparata and rhizoma alismatis is weighed by prescription amount to be sterilized, be ground into fine powder and mistake
Sieve；
(2) add the glucose oxidase of prescription amount, be uniformly mixed to get.
Embodiment 2-6 dosage of each component is increased or decreased according to same ratio, and the parts by weight relationship of gained each component is equal
It belongs to the scope of protection of the present invention.
The composition principle of Chinese medicine composition of the present invention is:
Fevervine extract is the effect for the treatment of in terms of the enterogastric diseases such as having indigestion, diarrhea, indigestion.
Rhizoma zingiberis has the effect of warming spleen and stomach for dispelling cold, recover Yang and smooth venation, warming lung and resolving fluid, is used for coldness and pain in the epigastrium, vomiting and diarrhea, limb cold arteries and veins
Micro-, cold drink is breathed with cough.
Radix Aconiti Lateralis Preparata is collapsed for yang depletion, cold weak pulse of limb, impotence, palace cold, trusted subordinate's crymodynia, cold of insufficiency type vomiting and diarrhea, cold sensation of the genitalia oedema, the deficiency of yang
Exogenous disease, numbness and ache of cold dampness, negative subcutaneous ulcer sore etc..
Rhizoma alismatis, which cures mainly, controls difficult urination, edema swelling, vomiting, dysentery etc..
Glucose oxidase is a kind of aerobic dehydrogenase, and oxidation of beta-D-Glucose that can be single-minded becomes gluconic acid and peroxide
Change hydrogen.Glucose oxidase has antibacterial, growth promoting function, can reduce pH value in animal gastrointestinal tract, thus inhibit harmful bacteria,
Promote the growth and breeding of beneficial bacterium, reduce animal diarrhea, improves growth of animal, there is similar acidulant.Grape is glycoxidative
The use of enzyme is considered as safe feed addictive, it is the natural products of microbial fermentation, is referred to as " green " addition
Agent.Currently, mainly having the research of glucose oxidase to animal intestinal tract development, the influence of production performance, and protected in food
Fresh and human medical application.
Each component synergistic effect considerably increases the bacteriostasis of fevervine extract, while entire composition is promoting
Livestock and poultry are long and treat significant effect in terms of enterogastric diseases.
Test example 1
1 fevervine extract of embodiment is to livestock and poultry encountered pathogenic bacteria body outer suppressioning test
The solution that fevervine extract is prepared is respectively to Pasteurella, salmonella, staphylococcus aureus and Fu Shi
The common animal pathogen of the livestock and poultry such as shigella dysenteriae carries out extracorporeal bacteria inhibitor test.By sterile working regulation, aseptically, match
The corresponding culture medium flat plate of strain processed draws each bacterium solution on Agar Plating, and coating is uniform.Dosing susceptability test paper piece is pasted,
Scraps of paper peace plate is tightly combined with aseptic nipper.Every part of sample does 3 parts, and results are averaged.Enrofloxacin medical fluid is used simultaneously
It compares.After 37 DEG C of constant incubator cultures for 24 hours, with vernier caliper measurement antibacterial circle diameter, judge medical fluid to Pasteurella, sand
The fungistatic effect of door Salmonella, staphylococcus aureus and Fu Shi shigella dysenteriae, judges susceptibility.
Judgment criteria: inhibition zone be greater than 20mm be it is extremely sensitive, 15-20mm Gao Min, 10-15mm be in quick, 10mm or less
For muting sensitive, no inhibition zone is insensitive.
As a result detection shows: under the conditions of the concentration of fevervine extract and Enrofloxacin is 100ug/ml, Pasteurella,
The bacteriostatic diameter of salmonella, staphylococcus aureus and Fu Shi shigella dysenteriae is all larger than 10mm, shows good antibacterial
Effect, such as table 1-1.
Table 1-1 fevervine extract scraps of paper drug sensitive test result (unit: mm)
Composition described in embodiment 2 is to livestock and poultry encountered pathogenic bacteria body outer suppressioning test
Composition described in embodiment 2 is configured to required concentration, respectively to Pasteurella, salmonella, golden yellow Portugal
The common animal pathogen of the livestock and poultry such as grape coccus and Fu Shi shigella dysenteriae carries out extracorporeal bacteria inhibitor test.By sterile working regulation,
Under aseptic condition, the corresponding culture medium flat plate of strain is prepared, draws each bacterium solution on Agar Plating, coating is uniform.Patch
Dosing susceptability test paper piece is tightly combined scraps of paper peace plate with aseptic nipper.Every part of sample does 3 parts, and results are averaged.It uses simultaneously
Enrofloxacin medical fluid compares.After 37 DEG C of constant incubator cultures for 24 hours, with vernier caliper measurement antibacterial circle diameter, medical fluid is judged
To the fungistatic effect of Pasteurella, salmonella, staphylococcus aureus and Fu Shi shigella dysenteriae, susceptibility is judged.
Judgment criteria: inhibition zone be greater than 20mm be it is extremely sensitive, 15-20mm Gao Min, 10-15mm be in quick, 10mm or less
For muting sensitive, no inhibition zone is insensitive.
As a result detection shows: the concentration of composition described in embodiment 2 (in terms of Herba Paederiae extract) and Enrofloxacin is
Under the conditions of 100ug/ml, Pasteurella, salmonella, staphylococcus aureus and the bacteriostatic diameter of Fu Shi shigella dysenteriae are all larger than
10mm shows good bacteriostasis, such as table 1-2.
Composition scraps of paper drug sensitive test result (unit: mm) described in table 1-2 embodiment 2
It using tube dilution method, is compared with Enrofloxacin, measurement has the minimum inhibitory concentration of the medical fluid of bacteriostatic activity
(MIC).Composition described in embodiment 2 is test group, makees a series of continuous doubling dilutions in ordinary broth, then takes 37 DEG C of vibrations
Swing culture 6h for try bacterium, it is dilute to take 0.1ml, be inoculated in each drug test tube respectively, while doing no drug bacterial controls, 37 DEG C training
It supports for 24 hours.
Criterion: drug broth culture is limpid, is asepsis growth, is minimum antibacterial with the minimum concentration of asepsis growth
It spends (MIC).
The testing result of minimal inhibitory concentration shows: composition described in embodiment 2 is to Pasteurella, salmonella, golden yellow
4 kinds of livestock and poultry encountered pathogenic bacteria bacterial strains of color staphylococcus and Fu Shi shigella dysenteriae have good fungistatic effect, to gram sun
Property and Gram-negative bacteria have good fungistatic effect.It can be seen that composition described in embodiment 2 has the bacteriostasis of wide spectrum.Examination
It tests the result shows that the minimum fungistatic effect concentration of composition described in embodiment 2 is higher than Enrofloxacin group (table 2), as substitution antibiosis
The drug of element, source is natural extensively, Small side effects, remains less, has good market prospects.
Composition minimal inhibitory concentration MIC (unit: ug/ml) described in 2 embodiment 2 of table
Test example 2
Influence test of the composition described in embodiment 2 to Growth Performance of Weaning Piglets
Experimental design: selection (35 ± 3) age in days is weaned, weight is close, normotrophic Ternary Pig three way cross piglet 120
Head is randomly divided into 3 processing, 3 repetitions of each processing, each repetition 10 by the male and female principle of correspondence between group.Processing one: basis
Daily ration control group；Processing two: the Enrofloxacin of 100mg/kg is added；Processing three: the herbal mixture pre-mixing agent of 50mg/kg is added
(in terms of Herba Paederiae extract).All feeding and the consistent daily ration of control group, analysis statistics each processing every other day increase after the test
Weight, feedstuff-meat ratio, diarrhea rate situation etc..
Feeding management: test carries out under same colony house, same poultry raiser and same management mode, and feed mode is fixed
When non-quantitative fed 3 times day, free water with eating up in 30min as degree after feeding.On-test at the end of, be single with processing
Position carries out empty stomach weighing to pig, and feelings occur for feed intake, diarrhea number of days and the diarrhoea degree of record pig and other diseases during test
Condition calculates average daily gain, daily ingestion amount, feedstuff-meat ratio, diarrhea rate etc..
Influence of the 3 herbal mixture pre-mixing agent of table to piglet growth performance
Note: the completely not identical person of letter of going together in mark indicates significant difference (P < 0.05).Similarly hereinafter.
As shown in table 3, the average daily gain of 3 groups and average daily gain are followed successively by herbal mixture premix from high to low
Agent, positive controls (Enrofloxacin), blank control group.Compared with blank control group, herbal mixture pre-mixing agent is added in daily ration
Improve average feed intake 7.29%；Average daily gain 14.05%；Feedstuff-meat ratio has dropped 5.92% (p < 0.05).Positive control
Group, herbal mixture pre-mixing agent significantly reduce diarrhea rate (P < 0.05) compared with blank control group.The result shows that being added in daily ration
Herbal mixture pre-mixing agent improves the feed intake of piglet, daily gain, and reduces feedstuff-meat ratio and diarrhea rate, to effectively improve
The growth performance of piglet.Effect is suitable compared with the control group of antibiotic Enrofloxacin.
Test example 3
Influence test of the herbal mixture pre-mixing agent to broiler growth performance
Experimental design: 360 1 age in days Huang plumage Broiler chicks, weight are close, are randomly divided into 3 by the male and female principle of correspondence between group
Processing, 6 repetitions of each processing, each repetition 20.Processing one: basal diet control group；Processing two: add 25mg/kg's
Enrofloxacin；Processing three: the herbal mixture pre-mixing agent of 100mg/kg is added.All feeding is consistent with control group after the test
Daily ration, analysis count each processing every other day
Weight gain, feedstuff-meat ratio, death rate situation etc..
Feeding management: it carries out disinfection before test to henhouse, sink and hopper.It supports, is freely eaten using totally-enclosed ground is flat
And drinking-water, illumination in 24 hours carry out routine immunization.Its temperature, humidity, density, ventilation, disinfection, epidemic prevention, feeding standard, feeding
Number, excrement clearing times etc. are identical.Each group is under identical environment and rearing conditions, is observed and is noted down chicken group's
Diet and excrement discharge situation, death condition etc., once it found the abnormal situation processing in time, environment inspection, feed apparatus and field
Ground disinfection, periodic replacement padding, adjust temperature etc..
Influence of the 4 herbal mixture pre-mixing agent of table to meat chicken growth performance
Note: the completely not identical person of letter of going together in mark indicates significant difference (P < 0.05).Similarly hereinafter.
As shown in table 4, the average daily gain of 3 groups and average daily gain are followed successively by herbal mixture premix from high to low
Agent, positive controls (Enrofloxacin), blank control group.Compared with blank control group, herbal mixture pre-mixing agent is added in daily ration
Improve average feed intake 1.57%；Average daily gain 11.76%；Feedstuff-meat ratio has dropped 9.09% (p < 0.05).Positive control
Group, herbal mixture pre-mixing agent significantly reduce death toll (P < 0.05) compared with blank control group.The result shows that being added in daily ration
Herbal mixture pre-mixing agent improves the feed intake of broiler chicken, daily gain, and reduces feedstuff-meat ratio and death toll, to effectively improve
The growth performance of broiler chicken.Effect is suitable compared with the control group of antibiotic Enrofloxacin.
Test example 4
Controlling experiment of the composition described in embodiment 2 to salmonellosis
Certain chicken house is broken out to arrange white loose stools and the disease for cardinal symptom such as drowsiness June.Sick young most spirit are depressed, contracting
Neck closes mesh necking down, lethargic sleep, and slow-witted stand does not like activity, and chilly is cold, and villus is loose, and two wings are sagging；Initial stage diseased chicken only shows food
It is intended to reduce, after without eating；Crop is spoken in a low voice inanition, and some has gas；Diarrhea arranges white slurry sample excrement, and perianal is pasted by excrement,
Lump is formed after dry and hard, blocks anus, and when defecation utters a scream；Individual serious persons are dead because of expiratory dyspnea or acute sepsis
It dies；The chicken retarded growth of resistance to mistake, feather is dry, loose；There is foot joint enlargement in limping in the only a few chicken later period.It chooses
The bacterium colony smear for microscopic examination isolated is selected, is the gram-negative acapsular dialister bacterium of no brood cell, the blunt garden in both ends is how single scattered
?.It takes and cultivates the separation bacterium cultivated for 24 hours through 37 DEG C on plain agar inclined-plane, add sterile saline that bacterium solution is made, inhale 50 μ L, with
Equivalent S. pullonum polyvalent serum type positive serum makees plate agglutinating reaction test, and it is solidifying apparent particle occur in 2min
Collection, result judgement is positive reaction.It is identified as Salmonella pullorum disease.Group described in all daily drinking embodiments 2 of diseased chicken
The aqua that the concentration (in terms of Herba Paederiae extract) that object is configured to is 50ppm is closed, is used in conjunction 7 days.While treatment, to henhouse and
Ambient enviroment carries out thorough disinfection and is sprayed, and once a day, 7d is used in conjunction.Chicken group gradually recovers one's health after 2 weeks.
The above-mentioned detailed description that a kind of fevervine extract and its application are carried out referring to embodiment, be it is illustrative and
It is not restrictive, several embodiments can be enumerated according to limited range, therefore in the case where not departing from present general inventive concept
Change and modification, should belong within protection scope of the present invention.
Claims (5)
1. a kind of for treating the composition of gastrointestinal tract of livestock and fowls disease, it is characterised in that: by fevervine extract, rhizoma zingiberis, black attached
Son, rhizoma alismatis and glucose oxidase composition, 5-10 parts of fevervine extract, 3-10 parts of rhizoma zingiberis, Radix Aconiti Lateralis Preparata based on its parts by weight
3-15 parts, 5-10 parts of rhizoma alismatis, 3-10 parts of glucose oxidase；The fevervine extract contains the iridoid glycoside of 60-99%
Class is prepared by the following method to obtain:
(1) fevervine medicinal material is taken, 8-12 times is added and measures 75% volume ratio alcohol reflux extraction 1-3 hours, filter extracting solution, the dregs of a decoction
It repeats said extracted process 2-3 times, combined extract, is concentrated under reduced pressure, until the distilled water that 2-6 times of medicinal material amount is added makes without alcohol taste
Dissolution, centrifugation, obtains supernatant；
(2) D101 macroporous absorbent resin on supernatant, medicinal material and amount of resin ratio are that every 1kg medicinal material needs 200-400ml through anhydrous second
D101 macroreticular resin after alcohol swelling pretreatment, it is 1: 8-12 that resin column diameter height, which compares, and loading flow velocity is 1.0-2.0BV/h, elution
Flow velocity is 4-6BV/h；
(3) it after loading, first with distillation water elution resin column 2BV, discards and flows through liquid and water elution, then with 20-85% volume ratio
Ethanol-eluting resin column 6BV, collects the eluent of ethyl alcohol, and 60 DEG C of reduced pressures are dry to get fevervine extract.
2. according to claim 1 for treating the composition of gastrointestinal tract of livestock and fowls disease, it is characterised in that: the fevervine
Extract is prepared by the following method to obtain:
(1) fevervine medicinal material is taken, 10 times of 75% volume ratio alcohol refluxs of amount are added and extract 2 hours, filters extracting solution, the dregs of a decoction repeat
Said extracted process 3 times, combined extract, 60 DEG C of reduced pressures, until the distilled water that 5 times of medicinal material amounts are added makes to dissolve without alcohol taste,
Centrifugation, obtains supernatant；
(2) D101 macroporous absorbent resin on supernatant, medicinal material are that every 1kg medicinal material needs 300ml molten through dehydrated alcohol with amount of resin ratio
Swollen pretreated D101 macroreticular resin, it is 1: 8 that resin column diameter height, which compares, and loading flow velocity is 2.0BV/h, elution flow rate 6BV/h；
(3) it after loading, first with distillation water elution resin column 2BV, discards and flows through liquid and water elution, then with 30% volume ratio ethyl alcohol
Resin column 6BV is eluted, the eluent of ethyl alcohol is collected, 60 DEG C of reduced pressures are dry to get fevervine extract.
3. according to claim 1 for treating the composition of gastrointestinal tract of livestock and fowls disease, it is characterised in that: press its parts by weight
Number meter 6 parts of fevervine extract, 5 parts of rhizoma zingiberis, 10 parts of Radix Aconiti Lateralis Preparata, 7 parts of rhizoma alismatis, 5 parts of glucose oxidase.
4. for treating the preparation method of the composition of gastrointestinal tract of livestock and fowls disease described in one of claim 1-3, feature exists
In: specific preparation process is as follows:
(1) fevervine extract, rhizoma zingiberis, Radix Aconiti Lateralis Preparata and rhizoma alismatis is weighed by prescription amount to be sterilized, be ground into fine powder and be sieved；
(2) add the glucose oxidase of prescription amount, be uniformly mixed to get.
5. for treating the preparation method of the composition of gastrointestinal tract of livestock and fowls disease described in one of claim 1-3, feature exists
In: specific preparation process is as follows:
(1) fevervine extract, rhizoma zingiberis, Radix Aconiti Lateralis Preparata and rhizoma alismatis are weighed by prescription amount to be sterilized, crush, mix, obtains raw material
Medicine mixed powder；
(2) by bulk pharmaceutical chemicals mixed powder, add 8-12 times to measure water and impregnate 30-60 minutes, after decocting 60-90 minutes, be obtained by filtration primary
Decoct medical fluid and the dregs of a decoction；The dregs of a decoction that will be obtained, again plus suitable decocting is endured, and is obtained by filtration and secondary is decocted medical fluid；It repeats above-mentioned
Step is decocted medical fluid three times, gained medical fluid will be mixed three times, being concentrated under reduced pressure into concentrate relative density is 1.10-1.15；
(3) after concentrate lets cool, filters, the glucose oxidase of prescription amount is added, is uniformly mixed to obtain the final product.
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