CN103719623A - Traditional Chinese medicine feed additive capable of preventing or relieving stress syndrome of weaned pigs and preparation method of feed additive - Google Patents
Traditional Chinese medicine feed additive capable of preventing or relieving stress syndrome of weaned pigs and preparation method of feed additive Download PDFInfo
- Publication number
- CN103719623A CN103719623A CN201310729176.6A CN201310729176A CN103719623A CN 103719623 A CN103719623 A CN 103719623A CN 201310729176 A CN201310729176 A CN 201310729176A CN 103719623 A CN103719623 A CN 103719623A
- Authority
- CN
- China
- Prior art keywords
- feed additive
- extract
- turmeric
- chinese medicine
- chinese herbal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention discloses a traditional Chinese medicine feed additive capable of preventing or relieving stress syndrome of weaned pigs and a preparation method of the feed additive. The traditional Chinese medicine feed additive provided by the invention is mainly prepared from the following raw materials: majoram, turmeric and macleaya cordata. The traditional Chinese medicine feed additive provided by the invention also can be composed of the following major ingredients: a majoram extract, a turmeric extract and a macleaya cordata extract. The invention further discloses the method for preparing the traditional Chinese medicine feed additive. The traditional Chinese medicine feed additive provided by the invention is capable of effectively enhancing the organism immunity of the weaned pigs, improving the oxidation resistance of the weaned pigs, regulating the functions of the digestive tract and the stomach and intestine of the weaned pigs, promoting the development of the digestive organs and the recovering of the gastrointestinal mucosa and shortening the diarrhea process of the weaned pigs; finally, the stress syndrome of the weaned pigs is effectively relieved and the growth performance of the weaned pigs is remarkably improved.
Description
Technical field
The present invention relates to a kind of prevention or alleviate the additive that domestic animal stress syndrome, relate in particular to a kind of prevention or alleviate Chinese herbal feed additive that weanling pig stress syndrome and preparation method thereof, belong to prevention or treatment field that weanling pig stress syndrome.
Background technology
The pig farm piglet of the current intensive production of China was generally weaned 3～4 week age.Weaned piglet is an important link in Swine Production process, directly affects later growing.During wean, by piglet and sow separately, the feed of piglet becomes dry feed or particulate material from full milk daily ration, and now weanling pig is in strongly growing period, but digestive function and resistance against diseases are strong not again.Variation along with physiology, nutrition, environmental condition, piglet can be made stress reaction at once, can make piglet immunological function, circulating antibody level decline, cellular immune function is suppressed, cause that a series of Physiology and biochemistries such as the circulation of carcass inner blood, breathing, endocrine, nervous system change, even cause " early weaning piglet stress disease (PWSD) " of various pathologic-physiological reactions simultaneously.Piglet often shows as feed intake decline, indigestion, efficiency of feed utilization is low, disease-resistant and immunocompetence is poor, diarrhoea, growth retardation and the situation such as body condition is poor, and therefore part piglet becomes cad pig.Because the uncontrollability of Changes in weather adds raiser, generally take the pattern of high density culture for pursuing cultured output, therefore, in breeding process there is in various degree stress phenomenon inevitable in piglet.Due to stress, surpass billions of units to every year the direct economic loss that Chinese piglet aquaculture is brought.
At present, in clinical, be mainly that the control of the change, temperature control and other environment that consist of daily ration prevents the early weaning piglet stress syndrome.But the control of environment needs great number to drop into, and uses high trophism, high-adaptability weaned piglet material can increase feeding cost, and this is pig farm at home, particularly small-sized pig farm and rural area are raised family scattered and are difficult to reach.And the diarrhoea that treatment stress of baby pigs caused ablaction causes mainly by adopting all kinds of antibiotic formulations (as: gentamicin, Ciprofloxacin, Florfenicol etc.), and is added the medicated feed additives such as terramycin and is prevented and treated in feed.Antibiotic has the effect that promotes piglet growth and disease preventing and treating, to improving culture benefit, promote the development of poultry industry to play larger effect, yet use for a long time antibiotic not only can destroy original microbiota in animal alimentary canal, affect the normal development of animal, also easily cause the problems such as medicament residue, drug resistance, drug contamination, by food chain, affect the mankind's health, human survival is threatened.
Therefore, develop a kind of effect significantly, the piglet Antistress agent tool of stable, the low toxicity easy to use, safe of effect, environmental protection is of great significance.
Summary of the invention
Main purpose of the present invention is to provide a kind of Chinese herbal feed additive that can effectively prevent or alleviate weanling pig stress syndrome;
Two of object of the present invention is a kind of a kind of methods of preparing described prevention or alleviating the Chinese herbal feed additive that weanling pig stress syndrome that provide.
Above-mentioned purpose of the present invention is mainly achieved through the following technical solutions:
Effective prevention or alleviate the Chinese herbal feed additive that weanling pig stress syndrome, comprises that following raw material makes: only dysentery grass, turmeric and macleaya cordata.
In order to reach better prevention or to alleviate the effect that weanling pig stress syndrome, the weight portion of each raw material is preferably: only dysentery grass 4-8 part, turmeric 2-4 part and macleaya cordata 0.1-0.2 part; Preferred, the weight portion of each raw material is: stop 5 parts of dysentery grass, 4 parts, turmeric, 0.1 part of macleaya cordata.
Further, in described Chinese herbal feed additive, also can contain the raw element of KG and arginine; In order to realize better effect, it is 8-12 part and 4-8 part that KG and arginine are given birth to plain weight portion excellent respectively, and preferred, KG is 12 weight portions, and the raw element of arginine is 8 weight portions.
In addition, in described Chinese herbal feed additive, also can contain carrier; Described carrier is preferably yeast cell wall, and its weight portion is preferably 20-30 part.
In addition, in Chinese herbal feed additive of the present invention, each raw material also can replace with its extract, can reach identical technique effect, thus, the present invention also provides another can prevent or alleviate the Chinese herbal feed additive that weanling pig stress syndrome, comprises following component: only dysentery grass extract, Turmeric P.E and macleaya cordata extracts.
In order to reach better prevention or to alleviate the effect that weanling pig stress syndrome, the weight portion of each component preferably: dysentery grass extract 0.008-0.16 part only, Turmeric P.E 0.008-0.16 part, macleaya cordata extracts 0.005-0.01 part; Preferred, the weight portion of each component is: only dysentery grass extract is 0.01 part, 0.016 part of Turmeric P.E, 0.005 part of macleaya cordata extracts;
Described each taste Chinese medical extract is preferably solid powdery, and it is all 100% calculating by active principle that described umber calculates;
Further, also can contain the nutriment that comprises KG and the raw element of arginine in above-mentioned Chinese medicine additive, wherein the weight portion of KG is preferably 8～12 parts, and the raw plain weight portion of arginine is preferably 4～8 parts; Preferred, KG is 12 weight portions, and the raw element of arginine is 8 weight portions.
Further, the carrier in above-mentioned Chinese medicine additive comprises 20～30 parts of yeast cell walls.
Only dysentery grass extract, Turmeric P.E or Turmeric P.E described in the present invention can be bought and be obtained by various commercial sources, and its specification meets industry standard; As a reference, described extract also can extract and obtain with reference to following methods: will stop dysentery grass, turmeric or macleaya cordata with alcohol extract or by water boiling and extraction; Extracting liquid filtering, after filtrate merges, concentration and recovery ethanol is to thick medicinal extract.
Preferably, only dysentery grass, turmeric or macleaya cordata adopt 10-95% alcohol extract 1-3 time; Preferred, with 80% alcohol extract 2 times, wherein, and the 1st 80% ethanol by 12 times of weight of medicinal material, 85 ℃ are extracted 2h; The 2nd the 80% alcohol extract 1.5h by 10 times of weight of medicinal material.
The present invention utilizes the theory that modern molecular nutrition is learned and Traditional Chinese Medicine Anti stress be relevant; for clinical outstanding symptom and pathology link; from protection intestine of young pigs 26S Proteasome Structure and Function, alleviate wean the damage of intestine of young pigs, the aspects such as oxidative stress that alleviate body set about, provide that a kind of curative effect is outstanding, the Chinese herbal feed additive of no drug residue, the effective prevention that has no side effect, has no drug resistance or treatment stress of baby pigs caused ablaction syndrome.
The present invention is according to the principles of formulating prescriptions of Chinese medicine " expelling cold and relieving exterior syndrome, the removing toxic substances of promoting blood circulation and removing blood stasis, Eradicates wind, dampness elimination stop dysentery ", pathogenetic cognition and principle of reatment based on traditional Chinese veterinary medicine to stress of baby pigs caused ablaction syndrome, according to the etiology and pathogenesis of stress of baby pigs caused ablaction syndrome, compatibility obtains Chinese herbal feed additive of the present invention, and the principle of learning in conjunction with modern molecular nutrition, add the raw element of KG and arginine, to improve intestinal mucosa form, promoted the absorption of nutriment.Prescription of the present invention can be clearing heat and detoxicating, anti-inflammation, strengthen weanling pig immunity of organisms, improve oxidation resistance, regulate the alimentary canal gastrointestinal function of diarrhoea piglet, promote Development of Digestive Organs, promote the reparation of piglet gastrointestinal mucosa, shorten the grice diarrhoea course of disease, thereby extenuate the ablactation stress of piglet, improve piglet growth performance.
Only dysentery is careless: having another name called wild marjoram, Elsholtzia patrini garcke, south Sinkiang ziziphora herb, is Labiatae Origanum herbaceos perennial, has the effect of urine detumescence of relieving superficies by cooling, regulating QI to expel dampness; Only dysentery grass or its extract can strengthen the ability that body is removed free radical, weaken the degree of lipid peroxidation, improve body resistance to oxidation.
Turmeric: be the dry rhizome of zingiberaceous plant turmeric Curcuma longa L..Property is pungent, bitter, temperature.Return spleen, Liver Channel.Function cures mainly: broken blood gas, and inducing meastruation to relieve menalgia.For the chest side of body, twinge amenorrhoea, abdominal mass, rheumatism shoulder arm pain, tumbling and swelling.Turmeric is fragrant stomachic tonic, and the disinfective action of favourable biliary tract and liver be take and invigorated blood circulation as main, and holding concurrently can promoting the circulation of qi, has the short metabolism of cholagogic, anti-inflammatory, the multiple efficacies such as anti-oxidant.
Macleaya cordata (Macleaya cordata (wild) R.Br): be Papaveraceae Macleaya perennial plant, it mainly contains effective constituent is sanguinarine and the white red alkali of Lay of bending, there is the Sweeling-eliminating medicine powder stasis of blood, the removing toxic substances of Eradicates wind, the function of analgesia desinsection, macleaya cordata or its extract have anti-inflammation, improve intestinal health, prevent and treat the effect of diarrhoea.
KG (A-ketoglutarate; AKG): be glutamine (Glutamine; Gln) precursor; can be by promoting the metabolism of nitrogen to alleviate the metabolic stress in animal production process; can also protect daily ration amino acid to be utilized as energy through enteron aisle; can promote by improving intestinal mucosa form the absorption of nutriment, thereby boost productivity.
The raw element of arginine (AAA): at stress situation and special growth phase, in body, synthetic arginine can not meet the needs of physiological metabolism, arginic deficiency is the key factor of stress of baby pigs caused ablaction, and arginine is being repaired intestinal mucosa damage, strengthens immunity of organisms, promoted to play an important role in the various physiology courses such as cell division and secreting hormone.The deficiency of N-acetylglutamat (NAG) can cause piglet arginine to lack, but the deacylase activity of mammiferous enterocyte degraded NAG is very high, has limited the approach that improves arginine concentration by exogenous interpolation NAG bis-.AAA is the analog that in urea cycle, ornithine generates the intermediate N acetylglutamate (NAG) of citrulling, can Effective Regulation endogenous arginic synthetic.
The present invention found through experiments, to stop dysentery grass, turmeric together with macleaya cordata compatibility, three kinds of medicines have significant Synergistic effect, than other drug regimen, the combination of these three kinds of medicines can be more effective the growth performance of raising weanling pig, and have best Antidiarrheic effect, average daily gain significantly improves, material anharmonic ratio, diarrhea rate and the death rate all significantly reduce; Chinese herbal feed additive of the present invention can significantly improve the anti-oxidation function of weanling pig body, and the leucocyte of the weanling pig that effectively raises, lymphocyte and neutrophil leucocyte are total, significantly promotes the immunity of organisms of piglet.
Another one object of the present invention is to provide a kind of method of preparing described Chinese herbal feed additive;
When Chinese herbal feed additive is that while take that only dysentery grass, turmeric and macleaya cordata are as primary raw material, its preparation method comprises the following steps:
(1) will stop dysentery grass, turmeric and macleaya cordata alcohol extract; Extracting liquid filtering, after filtrate merges, concentration and recovery ethanol is to thick medicinal extract;
(2) by thick extract dry powdered, powder is mixed with carrier, obtain Chinese medicine crude extract powder;
(3) Chinese medicine crude extract powder is mixed with KG, the raw element of arginine and yeast cell wall, obtain.
Wherein, preferred, in step (1), will stop 10-95% alcohol extract 1-3 time of dysentery grass, turmeric and macleaya cordata; Preferred, with 80% alcohol extract 2 times, wherein, and the 1st 80% ethanol by 12 times of weight of medicinal material, 85 ℃ are extracted 2h, the 2nd the 80% alcohol extract 1.5h by 10 times of weight of medicinal material.
In step (2), adopt spray drying process or vacuum drying chamber by thick extract dry powdered; Powder and carrier are mixed into the solid state powder with bulk drug equivalent weight; Described carrier is preferably starch.
Mixing described in its step (2) or step (3) is preferably the rotating speed premixed 25 minutes that adopts 15 revs/min in mixing arrangement.
When Chinese herbal feed additive is that while take that only dysentery grass extract, Turmeric P.E and macleaya cordata extracts are as key component, its preparation method comprises the following steps:
(1) will stop dysentery grass extract, Turmeric P.E and macleaya cordata extracts and mix with carrier, obtain Chinese medicine crude extract powder;
(2) Chinese medicine crude extract powder is mixed with KG, the raw element of arginine and yeast cell wall, obtain.
Wherein, described only dysentery grass extract, Turmeric P.E or macleaya cordata extracts adopts the extract obtaining after 10-95% alcohol extract; Preferably, will stop 10-95% alcohol extract 1-3 time of dysentery grass, turmeric and macleaya cordata; Preferred, with 80% alcohol extract 2 times, wherein, and the 1st 80% ethanol by 12 times of weight of medicinal material, 85 ℃ are extracted 2h, the 2nd the 80% alcohol extract 1.5h by 10 times of weight of medicinal material.
In step (1), only dysentery grass extract, Turmeric P.E or macleaya cordata extracts powder and carrier are mixed into the solid state powder with bulk drug equivalent weight; Described carrier is preferably starch.
Mixing described in step (1) or step (2) be preferably in mixing arrangement, adopt the rotating speed premixed 25 minutes of 15 revs/min.
The application process of Chinese herbal feed additive of the present invention: Chinese herbal feed additive of the present invention is added in the perfect compound feed of pig, the addition of Chinese herbal feed additive in perfect compound feed is preferably 0.5% (w/w).
Experimental result to weanling pig Antidiarrheic effect and production performance shows, each group of Chinese herbal feed additive of the present invention has obtained higher daily gain within experimental period and day searches for food and lower feedstuff-meat ratio, and the diarrhea rate of medication group and the death rate are all less than blank group and medicine control group.Medicine control sample is respectively organized daily gain, material anharmonic ratio is compared with blank group, also have some improvement, its diarrhea rate and the death rate are all less than blank group, but be starkly lower than Antidiarrheic effect and the production performance of each group of Chinese herbal feed additive of the present invention, this shows in Chinese herbal feed additive of the present invention that each components compatibility is together to promoting the growth of weanling pig and preventing that diarrhoea aspect from having played significant synergistic function.Illustrate to stop dysentery grass, turmeric and win three taste Chinese medicines and jointly play a role, can clearing heat and detoxicating, anti-inflammation, strengthen weanling pig cellular immune function, improve oxidation resistance, regulate the alimentary canal gastrointestinal function of diarrhoea piglet, promote Development of Digestive Organs, promote the reparation of piglet gastrointestinal mucosa, to shorten the grice diarrhoea course of disease, thereby extenuate the ablactation stress of piglet, improved piglet growth performance.
Power and the health degree of body defending system oxidation resistance exist substantial connection.Body Antioxidative Defense System mainly consists of antioxidant reductase and antioxidant, comprises the enzymes such as SOD, GSH-Px, CAT and VE, VC, carotenoid, amino acid etc.Between each composition of defense system, can mutually coordinate, mutually compensatory, interdepend and jointly bring into play antioxidation.The mensuration of T-AOC can reflect the antioxidant status of animal body all sidedly.Defense system comprises that SOD and GSH-Px in animal body play a very important role in the configuration aspects of removing free radical, anti-oxidative damage and maintaining cell.When interior free yl generation increases.SOD reacts with superoxide anion and generates H
2o
2, then be transformed into water by CAT and GSH-Px, thus free radical is eliminated, Cell protection is avoided damage.CAT is present in red blood cell and the interior microsome of histocyte, can catalysis H
2o
2change H into
2o and O
2.MDA is the end last metabolite of lipid peroxidation, the level of lipid peroxidation of its content reflection Mediated by Free Radicals.
Weanling pig oxidation resistance experimental result is shown, the oxidation resistance of Chinese herbal feed additive medicine group of the present invention is significantly higher than the oxidation resistance with medicine control group and blank group piglet, variation in conjunction with SOD, GSH-Px, CAT activity and MDA content, by only dysentery grass, turmeric can more effectively be improved the activity of piglet body radicals scavenging enzyme, the oxidation resistance of enhancing body together with winning three taste drug matchings.
Leucocyte is the main defence line that body is resisted the foreign body intrusions such as pathogenic microorganism, there is participation cellular immunity, engulf, produce antibody, the functions such as premunition information, total white blood cells directly reflects the immune state of body, and the height of neutrophil leucocyte quantity directly reflects the development of inflammation, lymphocyte is the most important part of participating in body's immunity in leucocyte, weanling pig Blood Physiological Indexes measurement result is shown, to stop dysentery grass, turmeric can more effectively increase total white blood cells and the lymphocyte quantity of weanling pig together with winning three taste drug matchings, illustrate that this Chinese medicine additive can increase the quantity of leucocyte in weanling pig peripheral blood, strengthen leukocytic immunity function.
Red blood cell is maximum a kind of of quantity in haemocyte, and red blood cell is considered to just transport O for a long time
2and CO
2instrument, modern medicine study finds, it can identify antigen, identification oneself and non-oneself; Remove immune complex in body; Strengthen cytophagous phagocytic function; There is the antigen effect of offering; The effect of performance effector cell sample.In red blood cell, contain hemoglobin, hemoglobin major function is to carry O
2and CO
2; Weanling pig Blood Physiological Indexes measurement result is shown, by only dysentery grass, turmeric can effectively be improved the erythrocyte number in weanling pig peripheral blood together with winning three taste drug matchings, content of hemoglobin is corresponding raising also, and this illustrates that this Chinese medicine additive has significant facilitation to the raising of the hematopoiesis function of weanling pig and immunity.
Chinese herbal feed additive of the present invention mainly contains the beneficial effect of following several respects:
(1) effectively improve the growth performance of weanling pig, and significantly strengthen the anti diar rhea ability of weanling pig: Chinese herbal feed additive of the present invention can significantly improve weanling pig average daily gain, reduce material anharmonic ratio, compare other medicines combination, Chinese herbal feed additive of the present invention can significantly reduce diarrhea rate and the death rate of weanling pig.
(2) significantly improve the anti-oxidation function of weanling pig body, effectively improve the anti-stress ability of weanling pig: add after Chinese herbal feed additive of the present invention, the activity of the activity of the TAC of weanling pig (T-AOC), glutathione peroxidase (GSH-PX), superoxide dismutase (SOD) activity and catalase (CAT) significantly improves, and the content of MDA (MDA) significantly reduces.
(3) effectively improve the immunity of organisms of weanling pig: can significantly raise weanling pig total white blood cells, lymphocyte number, neutrophil leucocyte number.
(4) significantly promote weanling pig red blood cell number and hemoglobin content: add after Chinese herbal feed additive of the present invention, the Chinese medicine additive group red blood cell number of weanling pig and hemoglobin have conspicuousness to raise, show that Chinese herbal feed additive of the present invention can promote animal body metabolism, the growth and development of body is had to positive effect, and this is also one of them pharmacological basis that this Chinese medicine additive improves weanling pig average daily gain.
(5) effectively improve weanling pig Mucosal Morphology in Small, alleviate the atrophy of weanling pig intestinal villi, promote enteron aisle reparation: add after Chinese herbal feed additive of the present invention, the duodenal height of naps of weanling pig is significantly increased, the crypts degree of depth all significantly reduces, and height of naps significantly improves with the ratio of the crypts degree of depth.
The specific embodiment
By the following example, embodiments of the present invention will be more specifically described, it should be understood that described embodiment is only exemplary, does not form any restriction to scope of the present invention.It will be understood by those skilled in the art that lower without departing from the spirit and scope of the present invention and can the details of technical solution of the present invention and form be modified or be replaced, but these modifications or replacement all fall into protection scope of the present invention.
The preparation of embodiment 1 Chinese herbal feed additive
According to following weight, get each bulk drug: stop dysentery grass 6.0kg, turmeric 3.0kg, macleaya cordata 0.1kg;
By after each bulk drug chopping, use for the first time 80% ethanol of 12 times of medicinal material weight, 85 ℃ are extracted 2h, use for the second time 80% alcohol extract 1.5h of 10 times of medicinal material weight, filter, merging filtrate, concentration and recovery ethanol, to thick medicinal extract, is used vacuum drying chamber that thick extract dry is become to powder, obtain Chinese medicine composition crude extract powder, add the solid state powder that starch is mixed with 9.1kg, in the mixing arrangement of 15 revs/min, premixed is 25 minutes, obtains Chinese medicine composition crude extract powder;
Take KG 10.0kg, the raw plain 6.0kg of arginine, yeast cell wall 24.9kg with the Chinese medicine composition crude extract powder having prepared together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min
The preparation of embodiment 2 Chinese herbal feed additives
According to following weight, get each bulk drug: stop dysentery grass 5.0kg, turmeric 4.0kg, macleaya cordata 0.1kg;
By after each bulk drug chopping, with 80% ethanol of 12 times of medicinal material amounts, 85 ℃ are extracted 2h for the first time, for the second time with 80% alcohol extract 1.5h of 10 times of medicinal material amounts, filter, merging filtrate, concentration and recovery ethanol, to thick medicinal extract, is used vacuum drying chamber that thick extract dry is become to powder, obtain Chinese medicine composition crude extract powder, add the solid state powder that starch is mixed with 9.1kg, in the mixing arrangement of 15 revs/min, premixed is 25 minutes, obtains Chinese medicine composition crude extract powder;
Take KG 12.0kg, the raw plain 8.0kg of arginine, yeast cell wall 20.9kg with the Chinese medicine composition crude extract powder having prepared together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min;
The preparation of embodiment 3 Chinese herbal feed additives
According to following weight, get each component: stop dysentery grass extract 0.012kg, Turmeric P.E 0.012kg, macleaya cordata extracts 0.005kg; Take after each taste Chinese medical extract, add the solid state powder that starch is mixed with 9.1kg, with starch together, in the mixing arrangement of 15 revs/min, premixed is 25 minutes, obtains Chinese medicine composition extract powder for each taste extract;
Take KG 10.0kg, the raw plain 6.0kg of arginine, yeast cell wall 24.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min;
The preparation of embodiment 4 Chinese herbal feed additives
According to following weight, get each component: stop dysentery grass extract 0.010kg, Turmeric P.E 0.016kg, macleaya cordata extracts 0.005kg; Take after each taste Chinese medical extract, add the solid state powder that starch is mixed with 9.1kg, with starch together, in the mixing arrangement of 15 revs/min, premixed is 25 minutes, obtains Chinese medicine composition extract powder for each taste extract;
Take KG 12.0kg, the raw plain 8.0kg of arginine, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
The preparation of embodiment 5 Chinese herbal feed additives
According to following weight, get each bulk drug: stop dysentery grass 4.0kg, turmeric 2.0kg, macleaya cordata 0.1kg;
By after each bulk drug chopping, use for the first time 80% ethanol of 12 times of medicinal material weight, 85 ℃ are extracted 2h, use for the second time 80% alcohol extract 1.5h of 10 times of medicinal material weight, filter, merging filtrate, concentration and recovery ethanol, to thick medicinal extract, is used vacuum drying chamber that thick extract dry is become to powder, obtain Chinese medicine composition crude extract powder, add the solid state powder that starch is mixed with 9.1kg, in the mixing arrangement of 15 revs/min, premixed is 25 minutes, obtains Chinese medicine composition crude extract powder;
Take KG 10.0kg, the raw plain 6.0kg of arginine, yeast cell wall 24.9kg with the Chinese medicine composition crude extract powder having prepared together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min
The preparation of embodiment 6 Chinese herbal feed additives
According to following weight, get each bulk drug: stop dysentery grass 8.0kg, turmeric 4.0kg, macleaya cordata 0.2kg;
By after each bulk drug chopping, use for the first time 80% ethanol of 12 times of medicinal material weight, 85 ℃ are extracted 2h, use for the second time 80% alcohol extract 1.5h of 10 times of medicinal material weight, filter, merging filtrate, concentration and recovery ethanol, to thick medicinal extract, is used vacuum drying chamber that thick extract dry is become to powder, obtain Chinese medicine composition crude extract powder, add the solid state powder that starch is mixed with 9.1kg, in the mixing arrangement of 15 revs/min, premixed is 25 minutes, obtains Chinese medicine composition crude extract powder;
Take KG 10.0kg, the raw plain 6.0kg of arginine, yeast cell wall 24.9kg with the Chinese medicine composition crude extract powder having prepared together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min
The preparation of embodiment 7 Chinese herbal feed additives
According to following weight, get each component: stop dysentery grass extract 0.008kg, Turmeric P.E 0.008kg, macleaya cordata extracts 0.005kg; Take after each taste Chinese medical extract, add the solid state powder that starch is mixed with 9.1kg, with starch together, in the mixing arrangement of 15 revs/min, premixed is 25 minutes, obtains Chinese medicine composition extract powder for each taste extract;
Take KG 12.0kg, the raw plain 8.0kg of arginine, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
The preparation of embodiment 8 Chinese herbal feed additives
According to following weight, get each component: stop dysentery grass extract 0.16kg, Turmeric P.E 0.16kg, macleaya cordata extracts 0.01kg; Take after each taste Chinese medical extract, add the solid state powder that starch is mixed with 9.1kg, with starch together, in the mixing arrangement of 15 revs/min, premixed is 25 minutes, obtains Chinese medicine composition extract powder for each taste extract;
Take KG 12.0kg, the raw plain 8.0kg of arginine, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
Control Example 1
According to following weight, get each component: stop dysentery grass extract 0.012kg, macleaya cordata extracts 0.005kg; Take after each taste Chinese medical extract, add the solid state powder that starch is mixed with 9.1kg, with starch together, in the mixing arrangement of 15 revs/min, premixed is 25 minutes, obtains Chinese medicine composition extract powder for each taste extract;
Take KG 12.0kg, the raw plain 8.0kg of arginine, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
Control Example 2
According to following weight, get each component: Turmeric P.E 0.012kg, macleaya cordata extracts 0.005kg; Take after each taste Chinese medical extract, add the solid state powder that starch is mixed with 9.1kg, with starch together, in the mixing arrangement of 15 revs/min, premixed is 25 minutes, obtains Chinese medicine composition extract powder for each taste extract;
Take KG 12.0kg, the raw plain 8.0kg of arginine, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
Control Example 3
According to following weight, get each component: Turmeric P.E 0.012kg, only dysentery grass extract 0.012kg; Take after each taste Chinese medical extract, add the solid state powder that starch is mixed with 9.1kg, with starch together, in the mixing arrangement of 15 revs/min, premixed is 25 minutes, obtains Chinese medicine composition extract powder for each taste extract;
Take KG 12.0kg, the raw plain 8.0kg of arginine, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
Control Example 4
According to following weight, get component: stop dysentery grass extract 0.012kg; Take Chinese medicine and stop after dysentery grass extract, add the solid state powder that starch is mixed with 9.1kg, only with starch together, in the mixing arrangement of 15 revs/min, premixed is 25 minutes for dysentery grass extract, obtains Chinese medicine and stops dysentery grass extract powder;
Take KG 12.0kg, the raw plain 8.0kg of arginine, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
Control Example 5
According to following weight, get each component: macleaya cordata extracts 0.005kg; Take after Chinese medical extract, add the solid state powder that starch is mixed with 9.1kg, with starch together, in the mixing arrangement of 15 revs/min, premixed is 25 minutes for macleaya cordata extracts, obtains Chinese medicine macleaya cordata extracts powder;
Take KG 12.0kg, the raw plain 8.0kg of arginine, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
Control Example 6
According to following weight, get each component: Turmeric P.E 0.012kg; Take after Turmeric extract, add the solid state powder that starch is mixed with 9.1kg, in the mixing arrangement of 15 revs/min, premixed is 25 minutes, obtains Chinese medical extract powder;
Take KG 12.0kg, the raw plain 8.0kg of arginine, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
Control Example 7
The preparation of Radix Berberidis extract: select the root of barberry, clean, dry, pulverize, boiling 3 times, after decoction liquor is concentrated, dry, through high-pressure liquid phase chromatogram therapy determining, proves that extract Berberine content is 198g/kg.
According to following weight, get each component: stop dysentery grass extract 0.010kg, Radix Berberidis extract 0.016kg, macleaya cordata extracts 0.005kg; Take after three kinds of Chinese medical extracts, add the solid state powder that starch is mixed with 9.1kg, in the mixing arrangement of 15 revs/min, premixed is 25 minutes, obtains Chinese medical extract powder;
Take KG 12.0kg, the raw plain 8.0kg of arginine, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
Control Example 8
Rosmarinus officinalis extract is purchased from the gloomy source of Yuzhou of Henan book on Chinese herbal medicine natural products Co., Ltd.
According to following weight, get each component: stop dysentery grass extract 0.010kg, Rosmarinus officinalis extract 0.016kg, macleaya cordata extracts 0.005kg; Take after three kinds of Chinese medical extracts, add the solid state powder that starch is mixed with 9.1kg, in the mixing arrangement of 15 revs/min, premixed is 25 minutes, obtains Chinese medical extract powder;
Take KG 12.0kg, the raw plain 8.0kg of arginine, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
Experimental example Chinese herbal feed additive of the present invention is in the effect experiment for the treatment of or alleviation stress of baby pigs caused ablaction syndrome
1 materials and methods
1.1 animals used as test and grouping
Animal used as test: select Du * length * Dasanyuan hybridization piglet 33 hurdles that 21 age in days wean, initial weight average are 6.55kg, 6, every hurdle, is divided into 11 groups, every group of 3 repetitions, the test period is 14d.
Basal diet is prepared according to NRC < < pig nutritional need > > (1998).
Experiment is divided into 12 groups, specific as follows:
1 blank group: do not add any additive except basal diet.
4 medicine groups: the Chinese herbal feed additive of medicine group 1(basal diet+embodiment 1), the Chinese herbal feed additive of medicine group 2(basal diet+embodiment 2), the Chinese herbal feed additive of medicine group 3(basal diet+embodiment 3), the Chinese herbal feed additive of medicine group 4(basal diet+embodiment 4);
8 control groups: the Chinese herbal feed additive of control group 1(basal diet+control Example 1), the Chinese herbal feed additive of control group 2(basal diet+control Example 2), the Chinese herbal feed additive of control group 3(basal diet+control Example 3), the Chinese herbal feed additive of control group 4(basal diet+control Example 4), the Chinese herbal feed additive of control group 5(basal diet+control Example 5), the Chinese herbal feed additive of control group 6(basal diet+control Example 6), the Chinese herbal feed additive of control group 7(basal diet+control Example 7), the Chinese herbal feed additive of control group 8(basal diet+control Example 8),
4 medicine groups and 8 control groups are except the kind of added Chinese medicine additive is different, and all the other are identical (comprising the addition of additive, composition of basal diet, the mode of feeding etc. are all identical);
The addition of the Chinese herbal feed additive of 4 medicine groups and 8 control groups in basal diet is 0.5%.Wet-mixing material is thrown something and fed, and carries out feeding experiment, and test is carried out 15d, piglet free choice feeding and drinking-water, conventional feeding and management and immunity.After wean, weigh, record piglets starting weight, test end is heavy; After record wean to diarrhea rate and the death rate during 35 ages in days; Each feed intake of processing of record, calculates average feed intake of full phase (ADFI), average daily gain (ADG) and material anharmonic ratio (F/G).
1.2 blood specimen collection
During off-test, morning, 8:00 weighed on an empty stomach, to every group each repeat to choose at random 3 pigs and carry out vena cava anterior blood sampling 10ml, wherein 2ml adds anticoagulant heparin, does the check of blood project, is stored in 4 ℃ of refrigerators, second day is tested; Remainder is at room temperature after standing 0.5h, and with 3000r/min centrifugation 15min, separation of serum ,-20 ℃ keep in Dark Place, for serum antioxidase index determining.
1.3 Serum antioxidant indices
TAC (T-AOC), colorimetric method; Superoxide dismutase (SOD): hydroxylamine assay; Glutathione peroxidase (GSH-Px): two sulfo-dinitrobenzoic acid (DTNB) direct measuring methods; MDA (MDA): TBA(thiobarbituricacidα-) method; Catalase (CAT): ultraviolet spectrophotometry; Kit builds up Bioengineering Research Institute by Nanjing and provides, and with UV762 type ultraviolet specrophotometer, measures.
1.4 statistical analysis
With the one-way ANOVA in SPSS13.0 statistical software, carry out the variance analysis of data, the multiple ratio of carrying out data with LSD check.
2 experimental results
The impact of 2.1 Chinese medicine additives on weanling pig Antidiarrheic effect and production performance
Table 1 Chinese medicine additive is to diarrhea of weaned piglets rate and death rate comparing result
From table 1: 1,2,3,4 groups of diarrhea rates of medicine group are respectively 0%, 5.6%, 0%, 0%,, the death rate is 0; 1,2,3,4,5,6,7,8 groups of diarrhea rates of control group are respectively 16.7%, 22.2%, 16.7%, 22.2%, 27.8%, 33.3%, 16.7% and 16.7%, and the death rate is respectively 11.1%, 11.1%, 11.1,16.7%, 16.7%, 16.7%, 5.6% and 11.1%; Blank group diarrhea rate is higher, is 55.6%, and the death rate is 27.8%.Medicine group can obviously reduce diarrhea rate and the death rate of piglet, and general curative effect is obviously better than control group and blank group.
Table 2 Chinese medicine additive is to weight gain of piglets effect experimental result
Note: the different alphabetical persons of colleague's data mark represent significant difference (p<0.05, Means ± SD).
From table 2, medicine group 1-4 group of the present invention weightening finish will significantly be increased (p<0.05) compared with control group, and each group weightening finish of medicine contrast is all significantly lower than medicine group of the present invention.
The impact of 2.2 Chinese medicine additives on weanling pig oxidation resistance
The impact of table 3 Chinese medicine additive on weanling pig activities of antioxidant enzymes
Note: the different alphabetical persons of colleague's data mark represent significant difference (p<0.05, Means ± SD).
From table 3: medicine is organized serum T-AOC activity at 1-4 and compared remarkable rising (p<0.05) with blank group, all has remarkable rising (p<0.05) compared with control group 1-8; Medicine 1-4 group activity of SOD in serum has been compared remarkable rising (p<0.05) with blank group, compared with control group 1-8, also have remarkable rising (p<0.05); Medicine group 1-4 group change of serum C AT enzymatic activity significantly raises (p<0.05) compared with blank group, compared with each group of control group 1-8, is also remarkable rising (p<0.05); Medicine group 1-4 group Content of MDA significantly reduces (p<0.05) compared with blank group; MDA content is respectively organized in contrast all reduction in various degree, but compares without significant difference (p>0.05) with blank group.
The impact of 2.3 Chinese medicine additives on weanling pig Blood Physiological Indexes
The impact of table 4 Chinese medicine additive on weanling pig Blood Physiological Indexes
Note: the different alphabetical persons of colleague's data mark represent significant difference (p<0.05, Means ± SD).
From table 4, medicine 1-4 group blood RBC content is compared remarkable rising (p<0.05) with blank group, the blood RBC content of control group 1-8 group raises to some extent, compares difference not remarkable (p>0.05) with blank group; Medicine 1-4 group blood HGB content is compared remarkable rising (p<0.05) with blank group, the blood HGB content of control group 1-8 group raises to some extent, compares difference not remarkable (p>0.05) with blank group; Medicine 1-4 group blood WBC content is compared remarkable rising (p<0.05) with blank group, the blood WBC content of control group 1-8 group raises to some extent, compares difference not remarkable (p>0.05) with blank group; Medicine 1-4 group LYM content is compared remarkable rising (p<0.05) with blank group, the LYM content of control group 1-8 group raises to some extent, compares difference not remarkable (p>0.05) with blank group; Each organizes NEU content without significant difference (p>0.05).
Claims (10)
1. prevention or alleviate the Chinese herbal feed additive that weanling pig stress syndrome, is characterized in that, comprises following bulk drug: only dysentery grass, turmeric and macleaya cordata.
2. according to Chinese herbal feed additive claimed in claim 1, it is characterized in that, the weight portion of each bulk drug is: stop dysentery grass 4-8 part, turmeric 2-4 part and macleaya cordata 0.1-0.2 part; Preferably, the weight portion of each raw material is: stop 5 parts of dysentery grass, 4 parts, turmeric, 0.1 part of macleaya cordata.
3. prevention or alleviate the Chinese herbal feed additive that weanling pig stress syndrome, is characterized in that, comprises following component: only dysentery grass extract, Turmeric P.E and macleaya cordata extracts.
4. according to Chinese herbal feed additive claimed in claim 3, it is characterized in that, the weight portion of each component is: stop dysentery grass extract 0.008-0.16 part, Turmeric P.E 0.008-0.16 part, macleaya cordata extracts 0.005-0.01 part; Preferably, the weight portion of each component is: only dysentery grass extract is 0.01 part, 0.016 part of Turmeric P.E, 0.005 part of macleaya cordata extracts.
5. the Chinese herbal feed additive described in any one according to claim 1-4, is characterized in that: also contain the raw element of KG and arginine; Preferably, the weight portion of KG is 8～12 parts, and the raw plain weight portion of arginine is 4～8 parts; Preferred, the weight portion of KG is 12 parts, and the raw plain weight portion of arginine is 8 parts.
6. the Chinese herbal feed additive described in any one according to claim 1-4, is characterized in that: also contain carrier; Described carrier is preferably yeast cell wall, and its weight portion is preferably 20～30 parts.
7. a method of preparing Chinese herbal feed additive described in claim 1 or 2, is characterized in that, comprises the following steps:
(1) will stop dysentery grass, turmeric and macleaya cordata alcohol extracting; Alcohol extract filters, and after filtrate merges, concentration and recovery ethanol is to thick medicinal extract;
(2) by thick extract dry powdered, powder is mixed with carrier starch, obtain Chinese medicine crude extract powder;
(3) Chinese medicine crude extract powder is mixed with KG, the raw element of arginine and yeast cell wall, obtain.
8. it is characterized in that in accordance with the method for claim 7: in step (1), will stop 10-95% alcohol extract 1-3 time of dysentery grass, turmeric and macleaya cordata; Preferably, with 80% alcohol extract 2 times, wherein, and the 1st 80% ethanol by 12 times of weight of medicinal material, 85 ℃ are extracted 2h, the 2nd the 80% alcohol extract 1.5h by 10 times of weight of medicinal material;
In step (2), adopt spray drying process or vacuum drying chamber by thick extract dry powdered; Powder and carrier are mixed into the solid state powder with bulk drug equivalent weight; Described carrier is preferably starch;
Being mixed into described in step (2) or step (3) adopts the rotating speed premixed 25 minutes of 15 revs/min in mixing arrangement.
9. a method of preparing Chinese herbal feed additive described in claim 3 or 4, is characterized in that, comprises the following steps:
(1) will stop dysentery grass extract, Turmeric P.E and macleaya cordata extracts and mix with carrier starch, obtain Chinese medicine crude extract powder;
(2) Chinese medicine crude extract powder is mixed with KG, the raw element of arginine and yeast cell wall, obtain.
The Chinese herbal feed additive of claim 1-4 described in any one preparation prevention or alleviate weanling pig stress syndrome medicament or feed in application.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310729176.6A CN103719623B (en) | 2013-12-26 | 2013-12-26 | Prevention or alleviate weanling pig stress the Chinese herbal feed additive and preparation method thereof of syndrome |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310729176.6A CN103719623B (en) | 2013-12-26 | 2013-12-26 | Prevention or alleviate weanling pig stress the Chinese herbal feed additive and preparation method thereof of syndrome |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103719623A true CN103719623A (en) | 2014-04-16 |
| CN103719623B CN103719623B (en) | 2015-08-19 |
Family
ID=50444117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310729176.6A Active CN103719623B (en) | 2013-12-26 | 2013-12-26 | Prevention or alleviate weanling pig stress the Chinese herbal feed additive and preparation method thereof of syndrome |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103719623B (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105231038A (en) * | 2015-09-23 | 2016-01-13 | 华南农业大学 | Application of alpha-oxoglutarate in respect of preparing pig feed additive |
| CN105394344A (en) * | 2015-11-27 | 2016-03-16 | 颍上县笑有生态农牧科技开发有限公司 | Feed Chinese herbal medicine capable of preventing stress syndrome |
| CN106538833A (en) * | 2016-11-24 | 2017-03-29 | 湖南美可达生物资源股份有限公司 | Application of the root of Herba Macleayae Cordatae in animal veterinary drug or medicated feed additive |
| CN107259155A (en) * | 2017-07-26 | 2017-10-20 | 漳州傲农牧业科技有限公司 | A kind of raising Growth Performance of Weaning Piglets simultaneously improves feed addictive of function of intestinal canal and its preparation method and application |
| CN107873987A (en) * | 2017-11-22 | 2018-04-06 | 漳州傲农牧业科技有限公司 | A kind of feed addictive for improving the gentle solution transport stress of fattening pig immunity and preparation method and application |
| CN108029859A (en) * | 2017-12-26 | 2018-05-15 | 广州美瑞泰科生物工程技术有限公司 | A kind of feed antioxidant and preparation method thereof |
| CN111759988A (en) * | 2020-07-16 | 2020-10-13 | 山西农业大学 | Traditional Chinese medicine preparation for improving immunity of weaned piglets and preparation method |
| CN112586607A (en) * | 2020-12-16 | 2021-04-02 | 长沙昆博生物科技有限公司 | Feed additive for improving color of animal carcass and application thereof |
| CN112617030A (en) * | 2020-12-10 | 2021-04-09 | 湖北雅琪生物科技有限公司 | Compound traditional Chinese medicine extract feed additive and preparation method thereof |
| CN113425676A (en) * | 2021-07-07 | 2021-09-24 | 河北农业大学 | Oregano macleaya cordata oral solution and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785536A (en) * | 2010-02-08 | 2010-07-28 | 阜阳市牧旺牧业有限公司 | Chinese herbal medicine compound feedstuff preparation eaten by broilers |
| CN102132767A (en) * | 2011-03-09 | 2011-07-27 | 北京桑普生物化学技术有限公司 | Curcumin or curcumin-ramification-containing composite feed additive and use thereof |
| CN102232467A (en) * | 2011-04-15 | 2011-11-09 | 北京康华远景科技有限公司 | Livestock and poultry anti-diarrheal feed additive and preparation method thereof |
| CN102450514A (en) * | 2010-12-06 | 2012-05-16 | 山东六和集团有限公司 | Compound crucian feed and preparation method thereof |
| CN102870984A (en) * | 2012-09-16 | 2013-01-16 | 湖南恒农生态农业发展有限公司 | Feed for anthony pigs and preparation method thereof |
-
2013
- 2013-12-26 CN CN201310729176.6A patent/CN103719623B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785536A (en) * | 2010-02-08 | 2010-07-28 | 阜阳市牧旺牧业有限公司 | Chinese herbal medicine compound feedstuff preparation eaten by broilers |
| CN102450514A (en) * | 2010-12-06 | 2012-05-16 | 山东六和集团有限公司 | Compound crucian feed and preparation method thereof |
| CN102132767A (en) * | 2011-03-09 | 2011-07-27 | 北京桑普生物化学技术有限公司 | Curcumin or curcumin-ramification-containing composite feed additive and use thereof |
| CN102232467A (en) * | 2011-04-15 | 2011-11-09 | 北京康华远景科技有限公司 | Livestock and poultry anti-diarrheal feed additive and preparation method thereof |
| CN102870984A (en) * | 2012-09-16 | 2013-01-16 | 湖南恒农生态农业发展有限公司 | Feed for anthony pigs and preparation method thereof |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105231038A (en) * | 2015-09-23 | 2016-01-13 | 华南农业大学 | Application of alpha-oxoglutarate in respect of preparing pig feed additive |
| CN105394344A (en) * | 2015-11-27 | 2016-03-16 | 颍上县笑有生态农牧科技开发有限公司 | Feed Chinese herbal medicine capable of preventing stress syndrome |
| CN106538833A (en) * | 2016-11-24 | 2017-03-29 | 湖南美可达生物资源股份有限公司 | Application of the root of Herba Macleayae Cordatae in animal veterinary drug or medicated feed additive |
| CN107259155A (en) * | 2017-07-26 | 2017-10-20 | 漳州傲农牧业科技有限公司 | A kind of raising Growth Performance of Weaning Piglets simultaneously improves feed addictive of function of intestinal canal and its preparation method and application |
| CN107873987A (en) * | 2017-11-22 | 2018-04-06 | 漳州傲农牧业科技有限公司 | A kind of feed addictive for improving the gentle solution transport stress of fattening pig immunity and preparation method and application |
| CN108029859A (en) * | 2017-12-26 | 2018-05-15 | 广州美瑞泰科生物工程技术有限公司 | A kind of feed antioxidant and preparation method thereof |
| CN111759988A (en) * | 2020-07-16 | 2020-10-13 | 山西农业大学 | Traditional Chinese medicine preparation for improving immunity of weaned piglets and preparation method |
| CN111759988B (en) * | 2020-07-16 | 2021-12-14 | 山西农业大学 | Traditional Chinese medicine preparation for improving immunity of weaned piglets and preparation method |
| CN112617030A (en) * | 2020-12-10 | 2021-04-09 | 湖北雅琪生物科技有限公司 | Compound traditional Chinese medicine extract feed additive and preparation method thereof |
| CN112586607A (en) * | 2020-12-16 | 2021-04-02 | 长沙昆博生物科技有限公司 | Feed additive for improving color of animal carcass and application thereof |
| CN113425676A (en) * | 2021-07-07 | 2021-09-24 | 河北农业大学 | Oregano macleaya cordata oral solution and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103719623B (en) | 2015-08-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103719623B (en) | Prevention or alleviate weanling pig stress the Chinese herbal feed additive and preparation method thereof of syndrome | |
| CN103125779B (en) | A kind of broiler chicken biological Chinese herbal medicine feed addictive and preparation method thereof | |
| CN102450515B (en) | Laying hen feed and traditional Chinese medicine feed additive used by same | |
| Hoseinifar et al. | Can dietary jujube (Ziziphus jujuba Mill.) fruit extract alter cutaneous mucosal immunity, immune related genes expression in skin and growth performance of common carp (Cyprinus carpio)? | |
| CN103583823A (en) | Mold removal agent for feed and preparation method and feed additive thereof | |
| CN102524579B (en) | Traditional Chinese medicine additive for pig feed and preparation method and application for traditional Chinese medicine additive | |
| CN103598426B (en) | For improving feed addictive and the feed of growth performance of chicks | |
| CN102078522A (en) | Chinese medicine composition for treating enterovirus syndrome in chicken | |
| CN104489420A (en) | Pig feed additive capable of booting immunity and improving meat quality | |
| CN104543375A (en) | Compound fermented Chinese herbal medicine preparation for livestock and poultry and preparation method and applications thereof | |
| CN107348136A (en) | A kind of vegetalitas promotees feed addictive and the application of weanling pig gastro-intestinal digestion function | |
| CN104543576B (en) | Prevent and treat composite vegetables extractive feed addictive and its preparation method application of grice diarrhoea | |
| CN101715893B (en) | Pure natural piglet immunological enhancement feed additive and application thereof | |
| CN106135749A (en) | Improve bait of Acipenser Sinensis meat and preparation method thereof | |
| CN102813056B (en) | Micro-biological degradation feed | |
| CN106511675A (en) | Medicine for preventing and treating poultry diseases and preparation method of medicine | |
| CN102318755A (en) | Traditional Chinese medicine immunity enhancer for aquatic animals | |
| CN104147586A (en) | Chinese herbal medicine composition and fodder for preventing and treating cold irritable enteritis of quails | |
| CN104324153A (en) | Traditional Chinese medicine composition for improving immunity of animals and preparation method thereof | |
| CN103892048A (en) | Feed additive with functions of soothing nerves, enhancing immunity and promoting growth for piglet and preparation method and application of additive | |
| CN106260521B (en) | Additive for farm animal feed and application thereof | |
| CN108041273A (en) | A kind of Chinese herbal composite feed additive for growing and fattening pigs | |
| CN105558350A (en) | Feed containing Chinese herbal medicine compound crude polysaccharide and preparation method of feed | |
| CN103263538B (en) | Traditional Chinese medicine combination with nourishing, anti-inflammation, antidiarrheal, hemostatic and analgesic effects for livestock and preparation method thereof | |
| KR101479619B1 (en) | A method for preparation of propolis by-product powder and feed additives comprising it |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210816 Address after: 437000 within the high tech Zone of Xianning City, Hubei Province Patentee after: Wuhan Huayang Tianle Biotechnology Co.,Ltd. Address before: No.15, Huanglongshan Road, Guannan East 2nd Park, Donghu hi tech Development Zone, Wuhan City, Hubei Province 430205 Patentee before: WUHAN HUAYANG ANIMAL PHARMACEUTICAL Co.,Ltd. |
|
| TR01 | Transfer of patent right |