CN103719623B - Prevention or alleviate weanling pig stress the Chinese herbal feed additive and preparation method thereof of syndrome - Google Patents
Prevention or alleviate weanling pig stress the Chinese herbal feed additive and preparation method thereof of syndrome Download PDFInfo
- Publication number
- CN103719623B CN103719623B CN201310729176.6A CN201310729176A CN103719623B CN 103719623 B CN103719623 B CN 103719623B CN 201310729176 A CN201310729176 A CN 201310729176A CN 103719623 B CN103719623 B CN 103719623B
- Authority
- CN
- China
- Prior art keywords
- feed additive
- chinese herbal
- extract
- herbal feed
- turmeric
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention discloses prevention or alleviate weanling pig stress the Chinese herbal feed additive and preparation method thereof of syndrome.Chinese herbal feed additive of the present invention is made primarily of following bulk drug: only dysentery grass, turmeric and macleaya cordata; Chinese herbal feed additive of the present invention also can be made up of following key component: only dysentery grass extract, Turmeric P.E and macleaya cordata extracts.The present invention further discloses the method preparing described Chinese herbal feed additive.Chinese herbal feed additive of the present invention can effectively strengthen weanling pig immunity of organisms, improve its oxidation resistance, regulate the alimentary canal gastrointestinal function of piglet, promote Development of Digestive Organs and gastrointestinal mucosa reparation, shorten the grice diarrhoea course of disease, final effectively extenuate weanling pig stress syndrome, significantly improve the growth performance of piglet.
Description
Technical field
The present invention relates to a kind of prevention or alleviate domestic animal stress the additive of syndrome, and particularly relating to a kind of prevention or alleviate weanling pig stress the Chinese herbal feed additive and preparation method thereof of syndrome, and belonging to weanling pig stress the prevention of syndrome or treatment field.
Background technology
The pig farm piglet of the current intensive manufacture of China was generally weaned 3 ~ 4 week age.Weaned piglet is an important link in Swine Production process, directly later the growing of impact.Piglet and sow are separated during wean, the feed of piglet becomes dry feed or particulate material from full milk daily ration, and now weanling pig is in intensive growth developmental stage, but digestive function and resistance against diseases are strong not again.Along with the change of physiology, nutrition, environmental condition, piglet can make stress reaction at once, piglet immunological function, circulating antibody level can be made to decline, cellular immune function is suppressed, cause a series of physiological acoustic signals such as the circulation of carcass inner blood, breathing, endocrine, nervous system, even cause " early weaning piglet stress disease (PWSD) " of various pathologic-physiological reaction simultaneously.Piglet often shows as feed intake decline, indigestion, efficiency of feed utilization is low, disease-resistant and immunocompetence is poor, diarrhoea, growth retardation and the situation such as body condition is poor, and therefore part piglet becomes cad pig.Uncontrollability due to Changes in weather adds that raiser generally takes the pattern of high density culture for pursuing cultured output, therefore, in breeding process piglet occur in various degree stress phenomenon inevitable.Due to stress, annual direct economic loss of bringing to Chinese piglet aquaculture exceedes billions of unit.
At present, the control of the change mainly consisted of daily ration in clinical, temperature control and other environment prevents early weaning piglet stress syndrome.But the control of environment needs great number to drop into, use high trophism, high-adaptability weaned piglet material can increase feeding cost, this is pig farm at home, and particularly small-sized pig farm and rural area are raised family scattered and be difficult to reach.And treat the diarrhoea that stress of baby pigs caused ablaction causes, then main by all kinds of antibiotic formulations of employing (as: gentamicin, Ciprofloxacin, Florfenicol etc.), and in feed, add the medicated feed additives such as terramycin prevent and treat.Antibiotic has the effect promoting piglet growth and disease preventing and treating, to raising culture benefit, promote that the development of poultry industry serves larger effect, but Long-Time Service antibiotic not only can destroy microbiota original in animal alimentary canal, affect the normal development of animal, also easily cause the problems such as medicament residue, drug resistance, drug contamination, affected the health of the mankind by food chain, human survival is threatened.
Therefore, develop that a kind of Be very effective, effect are stable, low toxicity easy to use, safe, environmental protection piglet Antistress agent tool be of great significance.
Summary of the invention
Main purpose of the present invention be to provide a kind of can effectively prevent or alleviate weanling pig stress the Chinese herbal feed additive of syndrome;
Two of object of the present invention a kind ofly provides that a kind of prepare described prevention or alleviate weanling pig stress the method for Chinese herbal feed additive of syndrome.
Above-mentioned purpose of the present invention is mainly achieved through the following technical solutions:
Effective prevention or alleviate weanling pig stress the Chinese herbal feed additive of syndrome, comprise following raw material and make: only dysentery grass, turmeric and macleaya cordata.
Stress the effect of syndrome in order to reach better prevention or alleviate weanling pig, the weight portion of each raw material is preferably: only dysentery grass 4-8 part, turmeric 2-4 part and macleaya cordata 0.1-0.2 part; Preferred, the weight portion of each raw material is: only dysentery grass 5 parts, 4 parts, turmeric, macleaya cordata 0.1 part.
Further, also can containing KG and the raw element of arginine in described Chinese herbal feed additive; In order to realize better effect, the weight portion of KG and the raw element of arginine is excellent is respectively 8-12 part and 4-8 part, and preferred, KG is 12 weight portions, and the raw element of arginine is 8 weight portions.
In addition, also carrier can be contained in described Chinese herbal feed additive; Described carrier is preferably yeast cell wall, and its weight portion is preferably 20-30 part.
In addition, in Chinese herbal feed additive of the present invention, each raw material also can replace with its extract, identical technique effect can be reached, thus, present invention also offers another can prevent or alleviate weanling pig stress the Chinese herbal feed additive of syndrome, comprise following component: only dysentery grass extract, Turmeric P.E and macleaya cordata extracts.
Stress the effect of syndrome in order to reach better prevention or alleviate weanling pig, the weight portion of each component preferably: only dysentery grass extract 0.008-0.16 part, Turmeric P.E 0.008-0.16 part, macleaya cordata extracts 0.005-0.01 part; Preferred, the weight portion of each component is: only dysentery grass extract 0.01 part, Turmeric P.E 0.016 part, macleaya cordata extracts 0.005 part;
Described each taste Chinese medical extract is preferably solid powdery, and it is all 100% calculating by active principle that described number calculates;
Further, also can containing the nutriment comprising KG and the raw element of arginine in above-mentioned Chinese medicine additive, wherein the weight portion of KG is preferably 8 ~ 12 parts, and the weight portion of the raw element of arginine is preferably 4 ~ 8 parts; Preferred, KG is 12 weight portions, and the raw element of arginine is 8 weight portions.
Further, the carrier in above-mentioned Chinese medicine additive comprises yeast cell wall 20 ~ 30 parts.
Only dysentery grass extract, Turmeric P.E or Turmeric P.E described in the present invention can be bought by various commercial sources and obtain, and its specification meets professional standard; As a reference, described extract also can refer to following methods and extracts and obtain: will stop that dysentery is careless, turmeric or macleaya cordata alcohol extract or by water boiling and extraction; Extracting liquid filtering, after filtrate merges, concentration and recovery ethanol is to thick medicinal extract.
Preferably, only dysentery grass, turmeric or macleaya cordata adopt 10-95% alcohol extract 1-3 time; Preferred, with 80% alcohol extract 2 times, wherein, the 1st 80% ethanol by medicinal material 12 times of weight, 85 DEG C are extracted 2h; 2nd the 80% alcohol extract 1.5h by medicinal material 10 times of weight.
The present invention utilizes the theory that modern molecular nutrition and Traditional Chinese Medicine Anti stress be relevant; for clinical outstanding symptom and pathology link; from protection intestine of young pigs 26S Proteasome Structure and Function, alleviate wean the damage of intestine of young pigs, the aspect such as oxidative stress that alleviates body set about, provide that a kind of curative effect is given prominence to, no drug residue, have no side effect, the Chinese herbal feed additive of effective prevention of having no drug resistance or treatment stress of baby pigs caused ablaction syndrome.
The present invention is according to the principles of formulating prescriptions of Chinese medicine " removing toxic substances of expelling cold and relieving exterior syndrome, promoting blood circulation and removing blood stasis, Eradicates wind, dampness elimination stop dysentery ", based on traditional Chinese veterinary medicine to pathogenetic cognition of stress of baby pigs caused ablaction syndrome and principle of reatment, according to the etiology and pathogenesis of stress of baby pigs caused ablaction syndrome, compatibility obtains Chinese herbal feed additive of the present invention, and in conjunction with the principle of modern molecular nutrition, with the addition of KG and the raw element of arginine, promote the absorption of nutriment to improve intestinal mucosa form.Prescription of the present invention can be clearing heat and detoxicating, anti-inflammation, strengthen weanling pig immunity of organisms, improve oxidation resistance, regulate the alimentary canal gastrointestinal function of diarrhoea piglet, promote Development of Digestive Organs, promote the reparation of piglet gastrointestinal mucosa, shorten the grice diarrhoea course of disease, thus extenuate the ablactation stress of piglet, improve piglet growth performance.
Only dysentery grass: having another name called wild marjoram, Elsholtzia patrini garcke, south Sinkiang ziziphora herb, is Labiatae Origanum herbaceos perennial, has effect that relieving superficies by cooling, regulating QI to expel dampness urinate detumescence; Only dysentery grass or its extract can strengthen the ability of body scavenging free radicals, weaken the degree of lipid peroxidation, improve body resistance to oxidation.
Turmeric: be the dry rhizome of zingiberaceous plant turmeric Curcuma longa L..Property pungent, bitter, temperature.Return spleen, Liver Channel.Function cures mainly: broken blood gas, inducing meastruation to relieve menalgia.Twinge for the chest side of body, amenorrhoea, abdominal mass, rheumatism shoulder arm pain, tumbling and swelling.Turmeric is the disinfective action of fragrant stomachic tonic, favourable biliary tract and liver, and to invigorate blood circulation, holding concurrently can promoting the circulation of qi, has cholagogic and urgees metabolism, anti-inflammatory, the multiple efficacies such as anti-oxidant.
Macleaya cordata (Macleaya cordata (wild) R.Br): be Papaveraceae Macleaya perennial plant, it mainly contains effective constituent is sanguinarine and the white red alkali of Lay in the wrong, there is the Sweeling-eliminating medicine powder stasis of blood, Eradicates wind detoxifies, the function of analgesia desinsection, the effect that macleaya cordata or its extract have anti-inflammation, improve intestinal health, prevent and treat diarrhoea.
KG (A-ketoglutarate; AKG): be glutamine (Glutamine; Gln) precursor; the metabolic stress alleviated in animal productiong process by promoting the metabolism of nitrogen; daily ration amino acid can also be protected to be utilized as energy through enteron aisle; the absorption of nutriment can be promoted by improving intestinal mucosa form, thus boost productivity.
The raw element (AAA) of arginine: at stress situation and special growth phase, in body, the arginine of synthesis can not meet the needs of physiological metabolism, arginic deficiency is the key factor of stress of baby pigs caused ablaction, and arginine plays an important role in the damage of reparation intestinal mucosa, enhancing immunity of organisms, various physiology course such as promotion cell division and secreting hormone etc.The deficiency of N-acetylglutamat (NAG) can cause piglet arginine to lack, but the deacylase activity of mammiferous enterocyte degraded NAG is very high, limits the approach being improved arginine concentrations by exogenous interpolation NAG bis-.AAA is the analog that in urea cycle, ornithine generates the intermediate N acetylglutamate (NAG) of citrulling, can the arginic synthesis of Effective Regulation endogenous.
The present invention found through experiments, dysentery grass, turmeric will be stopped together with macleaya cordata compatibility, three kinds of medicines have significant Synergistic effect, compared to other drug regimen, the combination of these three kinds of medicines can the growth performance of more effective raising weanling pig, and has best Antidiarrheic effect, and average daily gain significantly improves, feed-weight ratio, diarrhea rate and the death rate all significantly reduce; Chinese herbal feed additive of the present invention can significantly improve the anti-oxidation function of weanling pig body, effectively raises the leucocyte of weanling pig, lymphocyte and neutrophil leucocyte sum, significantly promotes the immunity of organisms of piglet.
Another one object of the present invention is to provide a kind of method preparing described Chinese herbal feed additive;
When Chinese herbal feed additive is that its preparation method comprises the following steps with when only dysentery grass, turmeric and macleaya cordata are for primary raw material:
(1) dysentery grass, turmeric and macleaya cordata alcohol extract will be stopped; Extracting liquid filtering, after filtrate merges, concentration and recovery ethanol is to thick medicinal extract;
(2) by thick extract dry powdered, powder is mixed with carrier, obtain Chinese medicine crude extract powder;
(3) Chinese medicine crude extract powder is mixed with KG, the raw element of arginine and yeast cell wall, to obtain final product.
Wherein, preferably, will stop that dysentery is careless in step (1), turmeric and macleaya cordata 10-95% alcohol extract 1-3 time; Preferred, with 80% alcohol extract 2 times, wherein, the 1st 80% ethanol by medicinal material 12 times of weight, 85 DEG C are extracted 2h, the 2nd the 80% alcohol extract 1.5h by medicinal material 10 times of weight.
Adopt spray drying process or vacuum drying chamber by thick extract dry powdered in step (2); Powder and carrier are mixed into the solid state powder with bulk drug equivalent weight; Described carrier is preferably starch.
Its step (2) or the mixing described in step (3) are preferably the rotating speed premixed 25 minutes adopting 15 revs/min in mixing arrangement.
When Chinese herbal feed additive is that its preparation method comprises the following steps with when only dysentery grass extract, Turmeric P.E and macleaya cordata extracts is for key component:
(1) dysentery grass extract, Turmeric P.E and macleaya cordata extracts will be stopped mix with carrier, obtain Chinese medicine crude extract powder;
(2) Chinese medicine crude extract powder is mixed with KG, the raw element of arginine and yeast cell wall, to obtain final product.
Wherein, the extract obtained after described only dysentery grass extract, Turmeric P.E or macleaya cordata extracts adopt 10-95% alcohol extract; Preferably, dysentery grass, turmeric and macleaya cordata 10-95% alcohol extract 1-3 time will be stopped; Preferred, with 80% alcohol extract 2 times, wherein, the 1st 80% ethanol by medicinal material 12 times of weight, 85 DEG C are extracted 2h, the 2nd the 80% alcohol extract 1.5h by medicinal material 10 times of weight.
By only dysentery grass extract, Turmeric P.E or macleaya cordata extracts powder and carrier are mixed into the solid state powder with bulk drug equivalent weight in step (1); Described carrier is preferably starch.
Step (1) or the mixing described in step (2) be preferably in the rotating speed premixed 25 minutes adopting 15 revs/min in mixing arrangement.
The application process of Chinese herbal feed additive of the present invention: add in the perfect compound feed of pig by Chinese herbal feed additive of the present invention, the addition of Chinese herbal feed additive in perfect compound feed is preferably 0.5% (w/w).
The experimental result of weanling pig Antidiarrheic effect and production performance is shown, each group of Chinese herbal feed additive of the present invention obtains higher daily gain and searches for food day and lower feedstuff-meat ratio within experimental period, and the diarrhea rate of medication group and the death rate are all less than blank group and drug control group.Drug control sample respectively organizes daily gain, feed-weight ratio compared with blank group, also have some improvement, its diarrhea rate and the death rate are all less than blank group, but be starkly lower than Antidiarrheic effect and production performance that Chinese herbal feed additive of the present invention respectively organizes, this shows that in Chinese herbal feed additive of the present invention, each components compatibility is together to promoting the growth of weanling pig and preventing diarrhoea aspect from serving significant synergistic function.Illustrate to stop dysentery grass, turmeric and win three taste Chinese medicines and jointly play a role, can clearing heat and detoxicating, anti-inflammation, strengthen weanling pig cellular immune function, improve oxidation resistance, regulate the alimentary canal gastrointestinal function of diarrhoea piglet, promote Development of Digestive Organs, promote the reparation of piglet gastrointestinal mucosa, to shorten the grice diarrhoea course of disease, thus extenuate the ablactation stress of piglet, improve piglet growth performance.
Power and the health degree of body defending system oxidation resistance also exist substantial connection.Body Antioxidative Defense System is formed primarily of antioxidant reductase and antioxidant, comprises the enzymes such as SOD, GSH-Px, CAT and VE, VC, carotenoid, amino acid etc.Can mutually coordinate between each composition of defense system, mutually compensatory, interdepend and jointly play antioxidation.The mensuration of T-AOC can reflect the antioxidant status of animal body all sidedly.Defense system comprises SOD and GSH-Px in animal body and all plays a very important role in the configuration aspects of scavenging free radicals, anti-oxidative damage and maintenance cell.When interior free yl generation increases.SOD and superoxide anion react and generate H
2o
2, then be transformed into water by CAT and GSH-Px, thus free radical is eliminated, Cell protection is from damage.CAT is present in red blood cell and histocyte in microsome, can catalysis H
2o
2change H into
2o and O
2.MDA is the end last metabolite of lipid peroxidation, the level of lipid peroxidation of its content reflection Mediated by Free Radicals.
Weanling pig oxidation resistance experimental result is shown, the oxidation resistance of Chinese herbal feed additive medicine group of the present invention is significantly higher than the oxidation resistance with drug control group and blank group piglet, the change of and MDA content active in conjunction with SOD, GSH-Px, CAT, by only dysentery grass, turmeric with win the activity more effectively can improving piglet body radicals scavenging enzyme together with three taste drug matchings, strengthen the oxidation resistance of body.
Leucocyte is the main defence line that body resists the foreign body intrusions such as pathogenic microorganism, there is participation cellular immunity, engulf, produce antibody, the functions such as premunition information, total white blood cells directly reflects the immune state of body, and the height of neutrophil leucocyte quantity directly reflects the development of inflammation, lymphocyte is the most important part of participating in body's immunity in leucocyte, weanling pig Blood Physiological Indexes measurement result is shown, dysentery grass will be stopped, turmeric with win total white blood cells and the lymphocyte quantity that more effectively can increase weanling pig together with three taste drug matchings, illustrate that this Chinese medicine additive can increase the quantity of leucocyte in weanling pig peripheral blood, strengthen leukocytic immunity function.
Red blood cell is the one that in haemocyte, quantity is maximum, and red blood cell is considered to just transport O for a long time
2and CO
2instrument, modern medicine study find, it can identify antigen, identify oneself and non-self; Immune complex in purged body; Strengthen cytophagous phagocytic function; There is Antigen-presenting role; Play the effect of effector cell's sample.Containing hemoglobin in red blood cell, hemoglobin major function is to carry O
2and CO
2; Weanling pig Blood Physiological Indexes measurement result is shown, by only dysentery grass, turmeric with win the erythrocyte number that can effectively to improve together with three taste drug matchings in weanling pig peripheral blood, content of hemoglobin is corresponding raising also, and this illustrates that this Chinese medicine additive has significant facilitation to the hematopoiesis function of weanling pig and the raising of immunity.
Chinese herbal feed additive of the present invention mainly contains the beneficial effect of following several respects:
(1) growth performance of weanling pig is effectively improved, and significantly strengthening the anti diar rhea ability of weanling pig: Chinese herbal feed additive of the present invention can significantly improve weanling pig average daily gain, reduce feed-weight ratio, compare other medicines combination, Chinese herbal feed additive of the present invention significantly can reduce diarrhea rate and the death rate of weanling pig.
(2) anti-oxidation function of weanling pig body is significantly improved, the anti-stress ability of effective raising weanling pig: after adding Chinese herbal feed additive of the present invention, the TAC (T-AOC) of weanling pig, the activity of glutathione peroxidase (GSH-PX), superoxide dismutase (SOD) activity that is active and catalase (CAT) significantly improves, and the content of MDA (MDA) significantly reduces.
(3) immunity of organisms of weanling pig is effectively improved: significantly can raise weanling pig total white blood cells, lymphocyte number, neutrophil leucocyte number.
(4) significantly weanling pig red blood cell number and hemoglobin content is promoted: after adding Chinese herbal feed additive of the present invention, the Chinese medicine additive group red blood cell number of weanling pig and hemoglobin have conspicuousness to raise, show that Chinese herbal feed additive of the present invention can promote animal body metabolism, have positive effect to the growth and development of body, this is also one of them pharmacological basis that this Chinese medicine additive improves weanling pig average daily gain.
(5) weanling pig Mucosal Morphology in Small is effectively improved, alleviate the atrophy of weanling pig intestinal villi, promote intestinal regeneration: after adding Chinese herbal feed additive of the present invention, the duodenal height of naps of weanling pig is significantly increased, Crypt depth all significantly reduces, and height of naps significantly improves with the ratio of Crypt depth.
Detailed description of the invention
More specifically embodiments of the present invention will be described by the following example, and it should be understood that described embodiment is only exemplary, any restriction is not formed to scope of the present invention.It will be understood by those skilled in the art that and can modify to the details of technical solution of the present invention and form or replace down without departing from the spirit and scope of the present invention, but these amendments or replacement all fall into protection scope of the present invention.
The preparation of embodiment 1 Chinese herbal feed additive
Each bulk drug is got: only dysentery grass 6.0kg, turmeric 3.0kg, macleaya cordata 0.1kg according to following weight;
After each bulk drug chopping, for the first time with 80% ethanol of medicinal material weight 12 times, 85 DEG C are extracted 2h, second time 80% alcohol extract 1.5h of medicinal material weight 10 times, filter, merging filtrate, concentration and recovery ethanol, to thick medicinal extract, uses vacuum drying chamber that thick extract dry is become powder, obtain Chinese medicine composition crude extract powder, add the solid state powder that starch is mixed with 9.1kg, in the mixing arrangement of 15 revs/min, premixed 25 minutes, obtains Chinese medicine composition crude extract powder;
Take KG 10.0kg, arginine raw plain 6.0kg, yeast cell wall 24.9kg, with the Chinese medicine composition crude extract powder prepared together, mix 25 minutes and get final product in the mixing arrangement of 15 revs/min
The preparation of embodiment 2 Chinese herbal feed additive
Each bulk drug is got: only dysentery grass 5.0kg, turmeric 4.0kg, macleaya cordata 0.1kg according to following weight;
After each bulk drug chopping, for the first time with 80% ethanol of medicinal material amount 12 times, 85 DEG C are extracted 2h, second time 80% alcohol extract 1.5h of medicinal material amount 10 times, filter, merging filtrate, concentration and recovery ethanol, to thick medicinal extract, uses vacuum drying chamber that thick extract dry is become powder, obtain Chinese medicine composition crude extract powder, add the solid state powder that starch is mixed with 9.1kg, in the mixing arrangement of 15 revs/min, premixed 25 minutes, obtains Chinese medicine composition crude extract powder;
Take KG 12.0kg, arginine raw plain 8.0kg, yeast cell wall 20.9kg, with the Chinese medicine composition crude extract powder prepared together, mix 25 minutes and get final product in the mixing arrangement of 15 revs/min;
The preparation of embodiment 3 Chinese herbal feed additive
Each component is got: only dysentery grass extract 0.012kg, Turmeric P.E 0.012kg, macleaya cordata extracts 0.005kg according to following weight; After taking each taste Chinese medical extract, add the solid state powder that starch is mixed with 9.1kg, with starch together, in the mixing arrangement of 15 revs/min, premixed 25 minutes, obtains Chinese medicine composition extract powder to each taste extract;
Take KG 10.0kg, arginine raw plain 6.0kg, yeast cell wall 24.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min;
The preparation of embodiment 4 Chinese herbal feed additive
Each component is got: only dysentery grass extract 0.010kg, Turmeric P.E 0.016kg, macleaya cordata extracts 0.005kg according to following weight; After taking each taste Chinese medical extract, add the solid state powder that starch is mixed with 9.1kg, with starch together, in the mixing arrangement of 15 revs/min, premixed 25 minutes, obtains Chinese medicine composition extract powder to each taste extract;
Take KG 12.0kg, arginine raw plain 8.0kg, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
The preparation of embodiment 5 Chinese herbal feed additive
Each bulk drug is got: only dysentery grass 4.0kg, turmeric 2.0kg, macleaya cordata 0.1kg according to following weight;
After each bulk drug chopping, for the first time with 80% ethanol of medicinal material weight 12 times, 85 DEG C are extracted 2h, second time 80% alcohol extract 1.5h of medicinal material weight 10 times, filter, merging filtrate, concentration and recovery ethanol, to thick medicinal extract, uses vacuum drying chamber that thick extract dry is become powder, obtain Chinese medicine composition crude extract powder, add the solid state powder that starch is mixed with 9.1kg, in the mixing arrangement of 15 revs/min, premixed 25 minutes, obtains Chinese medicine composition crude extract powder;
Take KG 10.0kg, arginine raw plain 6.0kg, yeast cell wall 24.9kg, with the Chinese medicine composition crude extract powder prepared together, mix 25 minutes and get final product in the mixing arrangement of 15 revs/min
The preparation of embodiment 6 Chinese herbal feed additive
Each bulk drug is got: only dysentery grass 8.0kg, turmeric 4.0kg, macleaya cordata 0.2kg according to following weight;
After each bulk drug chopping, for the first time with 80% ethanol of medicinal material weight 12 times, 85 DEG C are extracted 2h, second time 80% alcohol extract 1.5h of medicinal material weight 10 times, filter, merging filtrate, concentration and recovery ethanol, to thick medicinal extract, uses vacuum drying chamber that thick extract dry is become powder, obtain Chinese medicine composition crude extract powder, add the solid state powder that starch is mixed with 9.1kg, in the mixing arrangement of 15 revs/min, premixed 25 minutes, obtains Chinese medicine composition crude extract powder;
Take KG 10.0kg, arginine raw plain 6.0kg, yeast cell wall 24.9kg, with the Chinese medicine composition crude extract powder prepared together, mix 25 minutes and get final product in the mixing arrangement of 15 revs/min
The preparation of embodiment 7 Chinese herbal feed additive
Each component is got: only dysentery grass extract 0.008kg, Turmeric P.E 0.008kg, macleaya cordata extracts 0.005kg according to following weight; After taking each taste Chinese medical extract, add the solid state powder that starch is mixed with 9.1kg, with starch together, in the mixing arrangement of 15 revs/min, premixed 25 minutes, obtains Chinese medicine composition extract powder to each taste extract;
Take KG 12.0kg, arginine raw plain 8.0kg, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
The preparation of embodiment 8 Chinese herbal feed additive
Each component is got: only dysentery grass extract 0.16kg, Turmeric P.E 0.16kg, macleaya cordata extracts 0.01kg according to following weight; After taking each taste Chinese medical extract, add the solid state powder that starch is mixed with 9.1kg, with starch together, in the mixing arrangement of 15 revs/min, premixed 25 minutes, obtains Chinese medicine composition extract powder to each taste extract;
Take KG 12.0kg, arginine raw plain 8.0kg, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
Comparative examples 1
Each component is got: only dysentery grass extract 0.012kg, macleaya cordata extracts 0.005kg according to following weight; After taking each taste Chinese medical extract, add the solid state powder that starch is mixed with 9.1kg, with starch together, in the mixing arrangement of 15 revs/min, premixed 25 minutes, obtains Chinese medicine composition extract powder to each taste extract;
Take KG 12.0kg, arginine raw plain 8.0kg, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
Comparative examples 2
Each component is got: Turmeric P.E 0.012kg, macleaya cordata extracts 0.005kg according to following weight; After taking each taste Chinese medical extract, add the solid state powder that starch is mixed with 9.1kg, with starch together, in the mixing arrangement of 15 revs/min, premixed 25 minutes, obtains Chinese medicine composition extract powder to each taste extract;
Take KG 12.0kg, arginine raw plain 8.0kg, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
Comparative examples 3
Each component is got: Turmeric P.E 0.012kg, only dysentery grass extract 0.012kg according to following weight; After taking each taste Chinese medical extract, add the solid state powder that starch is mixed with 9.1kg, with starch together, in the mixing arrangement of 15 revs/min, premixed 25 minutes, obtains Chinese medicine composition extract powder to each taste extract;
Take KG 12.0kg, arginine raw plain 8.0kg, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
Comparative examples 4
Component is got: only dysentery grass extract 0.012kg according to following weight; Take after Chinese medicine stops dysentery grass extract, add the solid state powder that starch is mixed with 9.1kg, only dysentery grass extract with starch together, premixed 25 minutes in the mixing arrangement of 15 revs/min, obtains Chinese medicine and stop dysentery grass extract powder;
Take KG 12.0kg, arginine raw plain 8.0kg, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
Comparative examples 5
Each component is got: macleaya cordata extracts 0.005kg according to following weight; After taking Chinese medical extract, add the solid state powder that starch is mixed with 9.1kg, with starch together, in the mixing arrangement of 15 revs/min, premixed 25 minutes, obtains Chinese medicine macleaya cordata extracts powder to macleaya cordata extracts;
Take KG 12.0kg, arginine raw plain 8.0kg, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
Comparative examples 6
Each component is got: Turmeric P.E 0.012kg according to following weight; After taking Turmeric extract, add the solid state powder that starch is mixed with 9.1kg, in the mixing arrangement of 15 revs/min, premixed 25 minutes, obtains Chinese medical extract powder;
Take KG 12.0kg, arginine raw plain 8.0kg, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
Comparative examples 7
The preparation of Radix Berberidis extract: the root selecting barberry, cleans, dries, pulverizes, boiling 3 times, and decoction liquor is concentrated, after drying, through high-pressure liquid phase chromatogram therapy determining, prove that extract Berberine content is 198g/kg.
Each component is got: only dysentery grass extract 0.010kg, Radix Berberidis extract 0.016kg, macleaya cordata extracts 0.005kg according to following weight; After taking three kinds of Chinese medical extracts, add the solid state powder that starch is mixed with 9.1kg, in the mixing arrangement of 15 revs/min, premixed 25 minutes, obtains Chinese medical extract powder;
Take KG 12.0kg, arginine raw plain 8.0kg, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
Comparative examples 8
Rosmarinus officinalis extract is purchased from the gloomy source book on Chinese herbal medicine natural products Co., Ltd of Yuzhou of Henan.
Each component is got: only dysentery grass extract 0.010kg, Rosmarinus officinalis extract 0.016kg, macleaya cordata extracts 0.005kg according to following weight; After taking three kinds of Chinese medical extracts, add the solid state powder that starch is mixed with 9.1kg, in the mixing arrangement of 15 revs/min, premixed 25 minutes, obtains Chinese medical extract powder;
Take KG 12.0kg, arginine raw plain 8.0kg, yeast cell wall 20.9kg; Above-mentioned raw materials together, mixes 25 minutes and get final product in the mixing arrangement of 15 revs/min.
Experimental example Chinese herbal feed additive of the present invention is in treatment or the effect experiment alleviating stress of baby pigs caused ablaction syndrome
1 materials and methods
1.1 animals used as test and grouping
Animal used as test: Du × length × Dasanyuan hybridization piglet 33 hurdle selecting 21 age in days wean, original body mass average out to 6.55kg, 6, every hurdle, is divided into 11 groups, and often organize 3 repetitions, the test period is 14d.
Basal diet is prepared according to NRC " pig nutritional need " (1998).
Experiment is divided into 12 groups, specific as follows:
1 blank group: do not add any additive except basal diet.
4 medicine groups: the Chinese herbal feed additive of medicine group 1(basal diet+embodiment 1), the Chinese herbal feed additive of medicine group 2(basal diet+embodiment 2), the Chinese herbal feed additive of medicine group 3(basal diet+embodiment 3), the Chinese herbal feed additive of medicine group 4(basal diet+embodiment 4);
8 control groups: the Chinese herbal feed additive of control group 1(basal diet+comparative examples 1), the Chinese herbal feed additive of control group 2(basal diet+comparative examples 2), the Chinese herbal feed additive of control group 3(basal diet+comparative examples 3), the Chinese herbal feed additive of control group 4(basal diet+comparative examples 4), the Chinese herbal feed additive of control group 5(basal diet+comparative examples 5), the Chinese herbal feed additive of control group 6(basal diet+comparative examples 6), the Chinese herbal feed additive of control group 7(basal diet+comparative examples 7), the Chinese herbal feed additive of control group 8(basal diet+comparative examples 8),
4 medicine groups and 8 control groups except the kind of added Chinese medicine additive is different, all the other are identical (comprise the addition of additive, basal diet composition, feed mode etc. all identical);
The addition of Chinese herbal feed additive in basal diet of 4 medicine groups and 8 control groups is 0.5%.Wet-mixing material is thrown something and fed, and carries out feeding experiment, and test carries out 15d, piglet free choice feeding and drinking-water, conventional feeding and management and immunity.Weigh after wean, record piglets starting weight, test end is heavy; To the diarrhea rate during 35 ages in days and the death rate after record wean; Record the feed intake of each process, calculate average feed intake of full phase (ADFI), average daily gain (ADG) and feed-weight ratio (F/G).
1.2 blood specimen collection
During off-test, morning, 8:00 weighed on an empty stomach, and carry out vena cava anterior blood sampling 10ml to often organizing each repetition random selecting 3 pigs, wherein 2ml adds anticoagulant heparin, is used as blood item-test, is stored in 4 DEG C of refrigerators, within second day, tests; Remainder at room temperature leaves standstill after 0.5h, with 3000r/min centrifugation 15min, separation of serum ,-20 DEG C keep in Dark Place, for serum antioxidase index determining.
1.3 Serum antioxidant indices
TAC (T-AOC), colorimetric method; Superoxide dismutase (SOD): hydroxylamine assay; Glutathione peroxidase (GSH-Px): dithiobis-nitrobenzoic acid (DTNB) direct measuring method; MDA (MDA): TBA(thiobarbituricacidα-) method; Catalase (CAT): ultraviolet spectrophotometry; Kit builds up Bioengineering Research Institute by Nanjing and provides, and measures with UV762 type ultraviolet specrophotometer.
1.4 statistical analysis
Carry out the variance analysis of data with the one-way ANOVA in SPSS13.0 statistical software, carry out the Multiple range test of data with LSD inspection.
2 experimental results
2.1 Chinese medicine additives are on the impact of weanling pig Antidiarrheic effect and production performance
Table 1 Chinese medicine additive is to diarrhea of weaned piglets rate and death rate comparing result
From table 1: medicine group 1,2,3,4 groups of diarrhea rates are respectively 0%, 5.6%, 0%, 0%, the death rate is 0; Control group 1,2,3,4,5,6,7,8 groups of diarrhea rates are respectively 16.7%, 22.2%, 16.7%, 22.2%, 27.8%, 33.3%, 16.7% and 16.7%, and the death rate is respectively 11.1%, 11.1%, 11.1,16.7%, 16.7%, 16.7%, 5.6% and 11.1%; Blank group diarrhea rate is higher, is 55.6%, and the death rate is 27.8%.Medicine group obviously can reduce diarrhea rate and the death rate of piglet, and general curative effect is obviously better than control group and blank group.
Table 2 Chinese medicine additive is to weight gain of piglets effect experimental result
Note: the different alphabetical person of colleague's data mark represents significant difference (p<0.05, Means ± SD).
From table 2, the medicine group 1-4 group of the present invention comparatively control group that increases weight will significantly increase (p<0.05), and it is all remarkable in medicine group of the present invention that drug control respectively organizes weightening finish.
2.2 Chinese medicine additives are on the impact of weanling pig oxidation resistance
Table 3 Chinese medicine additive is on the impact of weanling pig activities of antioxidant enzymes
Note: the different alphabetical person of colleague's data mark represents significant difference (p<0.05, Means ± SD).
From table 3: medicine has remarkable rising (p<0.05) in 1-4 group serum T-AOC activity compared with blank group, and comparatively control group 1-8 all has remarkable rising (p<0.05); Medicine 1-4 group activity of SOD in serum has remarkable rising (p<0.05) compared with blank group, and comparatively control group 1-8 also has remarkable rising (p<0.05); Medicine group 1-4 group change of serum C AT enzymatic activity comparatively blank group significantly raises (p<0.05), and comparatively control group 1-8 respectively organizes also in significantly raising (p<0.05); Medicine group 1-4 group Content of MDA comparatively blank group significantly reduces (p<0.05); Contrast each group of MDA content and all have reduction in various degree, but without significant difference (p>0.05) compared with blank group.
2.3 Chinese medicine additives are on the impact of weanling pig Blood Physiological Indexes
Table 4 Chinese medicine additive is on the impact of weanling pig Blood Physiological Indexes
Note: the different alphabetical person of colleague's data mark represents significant difference (p<0.05, Means ± SD).
From table 4, the remarkable rising (p<0.05) compared with blank group of medicine 1-4 group blood RBC content, the blood RBC content of control group 1-8 group raises to some extent, and difference is not significantly (p>0.05) compared with blank group; The remarkable rising (p<0.05) compared with blank group of medicine 1-4 group blood HGB content, the blood HGB content of control group 1-8 group raises to some extent, and difference is not significantly (p>0.05) compared with blank group; The remarkable rising (p<0.05) compared with blank group of medicine 1-4 group blood WBC content, the blood WBC content of control group 1-8 group raises to some extent, and difference is not significantly (p>0.05) compared with blank group; The remarkable rising (p<0.05) compared with blank group of medicine 1-4 group LYM content, the LYM content of control group 1-8 group raises to some extent, and difference is not significantly (p>0.05) compared with blank group; Each group of NEU content is without significant difference (p>0.05).
Claims (9)
1. a prevention or alleviate weanling pig stress the Chinese herbal feed additive of syndrome, it is characterized in that, be made up of following composition: only dysentery grass 4-8 weight portion, turmeric 2-4 weight portion, macleaya cordata 0.1-0.2 weight portion, KG 8 ~ 12 weight portion, the raw element of arginine 4 ~ 8 weight portions, yeast cell wall 20 ~ 30 weight portion and carrier starch.
2. according to Chinese herbal feed additive according to claim 1, it is characterized in that, the weight portion of each composition is: only dysentery grass 5 parts, 4 parts, turmeric, macleaya cordata 0.1 part, KG 12 parts, the raw element 8 parts of arginine.
3. a prevention or alleviate weanling pig stress the Chinese herbal feed additive of syndrome, it is characterized in that, composed of the following components: only dysentery grass extract 0.008-0.16 weight portion, Turmeric P.E 0.008-0.16 weight portion, macleaya cordata extracts 0.005-0.01 weight portion, KG 8 ~ 12 weight portion, the raw element of arginine 4 ~ 8 weight portions, yeast cell wall 20 ~ 30 weight portion and carrier starch.
4. according to Chinese herbal feed additive according to claim 3, it is characterized in that, the weight portion of each component is: only dysentery grass extract 0.01 part, Turmeric P.E 0.016 part, macleaya cordata extracts 0.005 part, KG 12 parts, the raw element 8 parts of arginine.
5. prepare a method for Chinese herbal feed additive described in claim 1, it is characterized in that, comprise the following steps:
(1) dysentery grass, turmeric and macleaya cordata alcohol extracting will be stopped; Alcohol extract filters, and after filtrate merges, concentration and recovery ethanol is to thick medicinal extract;
(2) by thick extract dry powdered, powder is mixed with carrier starch, obtain Chinese medicine crude extract powder;
(3) Chinese medicine crude extract powder is mixed with KG, the raw element of arginine and yeast cell wall, to obtain final product.
6. in accordance with the method for claim 5, it is characterized in that: will stop that dysentery is careless in step (1), turmeric and macleaya cordata 10-95% alcohol extract 1-3 time;
Adopt spray drying process or vacuum drying chamber by thick extract dry powdered in step (2); Powder and carrier are mixed into the solid state powder with bulk drug equivalent weight;
Being mixed into described in step (2) or step (3) to adopt the rotating speed premixed 25 minutes of 15 revs/min in mixing arrangement.
7. in accordance with the method for claim 6, it is characterized in that: will stop that dysentery is careless in step (1), turmeric and macleaya cordata 80% alcohol extract 2 times, wherein, 1st 80% ethanol by medicinal material 12 times of weight, 85 DEG C are extracted 2h, the 2nd the 80% alcohol extract 1.5h by medicinal material 10 times of weight;
Carrier described in step (2) is starch.
8. prepare a method for Chinese herbal feed additive described in claim 3, it is characterized in that, comprise the following steps:
(1) dysentery grass extract, Turmeric P.E and macleaya cordata extracts will be stopped mix with carrier starch, obtain Chinese medicine crude extract powder;
(2) Chinese medicine crude extract powder is mixed with KG, the raw element of arginine and yeast cell wall, to obtain final product.
9. the Chinese herbal feed additive of claim 1-4 described in any one preparation prevention or alleviate weanling pig stress application in syndrome medicament or feed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310729176.6A CN103719623B (en) | 2013-12-26 | 2013-12-26 | Prevention or alleviate weanling pig stress the Chinese herbal feed additive and preparation method thereof of syndrome |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310729176.6A CN103719623B (en) | 2013-12-26 | 2013-12-26 | Prevention or alleviate weanling pig stress the Chinese herbal feed additive and preparation method thereof of syndrome |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103719623A CN103719623A (en) | 2014-04-16 |
| CN103719623B true CN103719623B (en) | 2015-08-19 |
Family
ID=50444117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310729176.6A Active CN103719623B (en) | 2013-12-26 | 2013-12-26 | Prevention or alleviate weanling pig stress the Chinese herbal feed additive and preparation method thereof of syndrome |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103719623B (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105231038A (en) * | 2015-09-23 | 2016-01-13 | 华南农业大学 | Application of alpha-oxoglutarate in respect of preparing pig feed additive |
| CN105394344A (en) * | 2015-11-27 | 2016-03-16 | 颍上县笑有生态农牧科技开发有限公司 | Feed Chinese herbal medicine capable of preventing stress syndrome |
| CN106538833A (en) * | 2016-11-24 | 2017-03-29 | 湖南美可达生物资源股份有限公司 | Application of the root of Herba Macleayae Cordatae in animal veterinary drug or medicated feed additive |
| CN107259155A (en) * | 2017-07-26 | 2017-10-20 | 漳州傲农牧业科技有限公司 | A kind of raising Growth Performance of Weaning Piglets simultaneously improves feed addictive of function of intestinal canal and its preparation method and application |
| CN107873987A (en) * | 2017-11-22 | 2018-04-06 | 漳州傲农牧业科技有限公司 | A kind of feed addictive for improving the gentle solution transport stress of fattening pig immunity and preparation method and application |
| CN108029859A (en) * | 2017-12-26 | 2018-05-15 | 广州美瑞泰科生物工程技术有限公司 | A kind of feed antioxidant and preparation method thereof |
| CN111759988B (en) * | 2020-07-16 | 2021-12-14 | 山西农业大学 | Traditional Chinese medicine preparation for improving immunity of weaned piglets and preparation method |
| CN112617030A (en) * | 2020-12-10 | 2021-04-09 | 湖北雅琪生物科技有限公司 | Compound traditional Chinese medicine extract feed additive and preparation method thereof |
| CN112586607A (en) * | 2020-12-16 | 2021-04-02 | 长沙昆博生物科技有限公司 | Feed additive for improving color of animal carcass and application thereof |
| CN113425676B (en) * | 2021-07-07 | 2022-12-30 | 河北农业大学 | Oregano macleaya cordata oral solution and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785536A (en) * | 2010-02-08 | 2010-07-28 | 阜阳市牧旺牧业有限公司 | Chinese herbal medicine compound feedstuff preparation eaten by broilers |
| CN102132767A (en) * | 2011-03-09 | 2011-07-27 | 北京桑普生物化学技术有限公司 | Curcumin or curcumin-ramification-containing composite feed additive and use thereof |
| CN102232467A (en) * | 2011-04-15 | 2011-11-09 | 北京康华远景科技有限公司 | Livestock and poultry anti-diarrheal feed additive and preparation method thereof |
| CN102450514A (en) * | 2010-12-06 | 2012-05-16 | 山东六和集团有限公司 | Compound crucian feed and preparation method thereof |
| CN102870984A (en) * | 2012-09-16 | 2013-01-16 | 湖南恒农生态农业发展有限公司 | Feed for anthony pigs and preparation method thereof |
-
2013
- 2013-12-26 CN CN201310729176.6A patent/CN103719623B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785536A (en) * | 2010-02-08 | 2010-07-28 | 阜阳市牧旺牧业有限公司 | Chinese herbal medicine compound feedstuff preparation eaten by broilers |
| CN102450514A (en) * | 2010-12-06 | 2012-05-16 | 山东六和集团有限公司 | Compound crucian feed and preparation method thereof |
| CN102132767A (en) * | 2011-03-09 | 2011-07-27 | 北京桑普生物化学技术有限公司 | Curcumin or curcumin-ramification-containing composite feed additive and use thereof |
| CN102232467A (en) * | 2011-04-15 | 2011-11-09 | 北京康华远景科技有限公司 | Livestock and poultry anti-diarrheal feed additive and preparation method thereof |
| CN102870984A (en) * | 2012-09-16 | 2013-01-16 | 湖南恒农生态农业发展有限公司 | Feed for anthony pigs and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103719623A (en) | 2014-04-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103719623B (en) | Prevention or alleviate weanling pig stress the Chinese herbal feed additive and preparation method thereof of syndrome | |
| Hoseinifar et al. | Can dietary jujube (Ziziphus jujuba Mill.) fruit extract alter cutaneous mucosal immunity, immune related genes expression in skin and growth performance of common carp (Cyprinus carpio)? | |
| CN102450515B (en) | Laying hen feed and traditional Chinese medicine feed additive used by same | |
| CN103583823A (en) | Mold removal agent for feed and preparation method and feed additive thereof | |
| CN103125779A (en) | Biological Chinese herbal feed additive for broiler chicken and preparation method thereof | |
| CN110100956A (en) | It is a kind of with anti-oxidant and immunoloregulation function " acid+essential oil " coating type feed addictive and its preparation method and application | |
| CN103598426B (en) | For improving feed addictive and the feed of growth performance of chicks | |
| CN103262943B (en) | A kind of Herba Lophatheri extract and application thereof | |
| CN102078522A (en) | Chinese medicine composition for treating enterovirus syndrome in chicken | |
| Guo et al. | Effects of the stems and leaves of Astragalus membranaceus on growth performance, immunological parameters, antioxidant status, and intestinal bacteria of quail | |
| CN106071402A (en) | Multiple elements design feed of grass carp based on Folium Mori and preparation method thereof | |
| CN104304679A (en) | Multivitamin C, E immune polysaccharide microemulsion preparation and preparation method and application of preparation | |
| CN114568602A (en) | Traditional Chinese medicine compound for improving growth performance and organism immunity of broiler chickens and preparation method and application thereof | |
| CN109497310A (en) | One boar combines Se-enriched feedstuff with Chinese herbal medicine | |
| CN103039725A (en) | Health-care chick starter feed | |
| CN104664168B (en) | A kind of piglet specific complex vitamin microemulsion preparation and its preparation method and application | |
| CN103892048B (en) | Piglet is calmed the nerves to increase and exempts from growth-promoting feedstuff additive, preparation method and application thereof | |
| Mansour et al. | Physiological and immunological responses of Nile tilapia fed dietary supplementation of sweet basil ethanolic and aqueous extracts | |
| CN102318755A (en) | Traditional Chinese medicine immunity enhancer for aquatic animals | |
| CN104324153A (en) | Traditional Chinese medicine composition for improving immunity of animals and preparation method thereof | |
| CN106260521B (en) | Additive for farm animal feed and application thereof | |
| CN106075241A (en) | A kind of piglet health care growth promotion Chinese medicine composition and preparation method thereof | |
| Chekav et al. | Effects of dietary Persian hogweed (Heracleum persicum) powder on growth performance, hematological and immune indices, and resistance against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss) | |
| CN105558350A (en) | Feed containing Chinese herbal medicine compound crude polysaccharide and preparation method of feed | |
| KR101479619B1 (en) | A method for preparation of propolis by-product powder and feed additives comprising it |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210816 Address after: 437000 within the high tech Zone of Xianning City, Hubei Province Patentee after: Wuhan Huayang Tianle Biotechnology Co.,Ltd. Address before: No.15, Huanglongshan Road, Guannan East 2nd Park, Donghu hi tech Development Zone, Wuhan City, Hubei Province 430205 Patentee before: WUHAN HUAYANG ANIMAL PHARMACEUTICAL Co.,Ltd. |
|
| TR01 | Transfer of patent right |