The specific embodiment
By describing technology contents of the present invention, structural feature in detail, realized purpose and effect, give explanation below in conjunction with embodiment is detailed.
The used raw material explanation of the present invention:
1, the Chinese bulbul: the Chinese bulbul is cohosh Chinese bulbul perennial root, available from Fujian with aphrodisiac industry limited company.Appraisal basis is " two ones of People's Republic of China's veterinary drug allusion quotations " 2005 editions " Chinese bulbul " medicinal material standards.
2, Poria cocos: available from Fujian with aphrodisiac industry limited company.Appraisal basis is " two ones of People's Republic of China's veterinary drug allusion quotations " 2005 editions " Poria cocos " medicinal material standards.
3, the bighead atractylodes rhizome: be the rhizome of the composite family [WTBX plant bighead atractylodes rhizome, contain volatile oil (atractylone, atractylol, atractyloxide, juniper camphor, atractyloide etc.), fructose, synanthrin, soluble polysaccharide, several amino acids and vitamin A etc.Available from Fujian with aphrodisiac industry limited company.Appraisal basis is " two ones of People's Republic of China's veterinary drug allusion quotations " 2005 editions " bighead atractylodes rhizome " medicinal material standards.
4, contracting fructus amomi: the place of production is North China, northeast and Shandong, Shaanxi, Gansu, Ningxia, is the root of Umbelliferae contracting Amomum plant contracting fructus amomi.Available from Fujian with aphrodisiac industry limited company.Appraisal basis is " two ones of People's Republic of China's veterinary drug allusion quotations " 2005 editions " contracting fructus amomi " medicinal material standards.
5, Radix Codonopsis: the root for Campanulaceae Codonopsis plant Radix Codonopsis, plain flower Radix Codonopsis, radix codonpsis tangshen, pipe flower Radix Codonopsis, globe daisy Radix Codonopsis, gray wool Radix Codonopsis contains sterol, tangshenoside, Codonopsis pilosula polysaccharide, codonolactone, alkaloid etc.Available from Fujian with aphrodisiac industry limited company.Appraisal basis is " two ones of People's Republic of China's veterinary drug allusion quotations " 2005 editions " Radix Codonopsis " medicinal material standards.
6, dried orange peel: the maturate peel of rutaceae orange and variety thereof.Available from Fujian with aphrodisiac industry limited company.Appraisal basis is " two ones of People's Republic of China's veterinary drug allusion quotations " 2005 editions " dried orange peel " medicinal material standards.
7, the tuber of pinellia: the dry mature fruit of rosaceous plant large-fruited Chinese hawthorn or the tuber of pinellia.Available from Fujian with aphrodisiac industry limited company.Appraisal basis is " two ones of People's Republic of China's veterinary drug allusion quotations " 2005 editions " tuber of pinellia " medicinal material standards.
8, wrinkled giant hyssop: be the dry herb of Labiatae herbaceos perennial wrinkled giant hyssop.Available from Fujian with aphrodisiac industry limited company.Appraisal basis is " two ones of People's Republic of China's veterinary drug allusion quotations " 2005 editions " wrinkled giant hyssop " medicinal material standards.
9, balloonflower root: be the dried roots of Campanulaceae Platycodon grandiflouorum.Available from Fujian with aphrodisiac industry limited company.Appraisal basis is " two ones of People's Republic of China's veterinary drug allusion quotations " 2005 editions " balloonflower root " medicinal material standards.
10, honey-fried licorice root: for the honey of glycyrrhizic legume is smoked processed goods.Available from Fujian with aphrodisiac industry limited company.Appraisal basis is " two ones of People's Republic of China's veterinary drug allusion quotations " 2005 editions " Radix Glycyrrhizae " medicinal material standards.
11, rhizoma zingiberis: the dry rhizome of zingiber, available from Fujian with aphrodisiac industry limited company.Appraisal basis is " two ones of People's Republic of China's veterinary drug allusion quotations " 2005 editions " rhizoma zingiberis " medicinal material standards.
Embodiment 1 pig feed Chinese medicine additive prescription of the present invention and preparation method
Prescription one
The weight part ratio of each raw material components is as follows in the additive: 5 parts of the Chinese bulbuls, 3 parts in Poria cocos, 3 parts of contracting fructus amomis, 3 parts of Radix Codonopsis, 3 parts of the bighead atractylodes rhizomes.
Prescription two
The weight part ratio of each raw material components is as follows in the additive: 8 parts of the Chinese bulbuls, 5 parts in Poria cocos, 5 parts of contracting fructus amomis, 5 parts of Radix Codonopsis, 5 parts of the bighead atractylodes rhizomes.
Prescription three
The weight part ratio of each raw material components is as follows in the additive: 5 parts of the Chinese bulbuls, 3 parts in Poria cocos, contracting fructus amomi 3-5 part, 3 parts of Radix Codonopsis, 3 parts of the bighead atractylodes rhizomes, 5 parts of dried orange peels, 5 parts of the tuber of pinellia, 4 parts of wrinkled giant hyssops, 3 parts of balloonflower roots, 3 parts of honey-fried licorice roots, 2 parts of rhizoma zingiberis.
Prescription four
The weight part ratio of each raw material components is as follows in the additive: 8 parts of the Chinese bulbuls, 5 parts in Poria cocos, 5 parts of contracting fructus amomis, 5 parts of Radix Codonopsis, 5 parts of the bighead atractylodes rhizomes, 5 parts of dried orange peels, 5 parts of the tuber of pinellia, 4 parts of wrinkled giant hyssops, 3 parts of balloonflower roots, 3 parts of honey-fried licorice roots, 2 parts of rhizoma zingiberis.
Prescription five
The weight part ratio of each raw material components is as follows in the additive: the Chinese bulbul 16 grams, Radix Codonopsis 10 grams, contracting fructus amomi 10 grams, the bighead atractylodes rhizome 10 grams, Poria cocos 10 grams, the tuber of pinellia 10 grams, dried orange peel 10 grams, wrinkled giant hyssop 8 grams, balloonflower root 6 grams, honey-fried licorice root 6 grams, rhizoma zingiberis 4 grams.
Method with the feedstock production pig feed Chinese medicine additive of above prescription may further comprise the steps:
Each raw material of a, the above prescription of weighing.
B, with half of every kind of raw material of step a, pour into the equivalent incremental method and to carry out one-level in the efficient mixer and mix, rotating speed 12r/min mixed 10 minutes, the back is dropped into remaining raw material and was mixed the shutdown discharging 20 minutes.
C, drying: being dried to moisture is 3%, pulverizes according to the requirement of tcm and herbal slice powder, and 3 minutes pulverizing time, the rate 80% that feeds intake is crossed sieve No. two.
Clinical 4 result of the tests of embodiment 2 pig feed Chinese medicine additives of the present invention and analysis
Pig feed Chinese medicine additive of the present invention is powder, and wherein related technology and used pharmaceutical standards all are routine techniques well known to those skilled in the art unless stated otherwise.Referring to for example " veterinary drug stability test guideline " (appendix of Chinese veterinary drug allusion quotation version in 2005),
Pig syndrome of deficiency of spleen qi mosel methodology is inquired into the influence to the empty pork pig blood parameters of temper and immune index of correlation.This experiment is used in the syndrome of deficiency of spleen qi model of pig, prepare syndrome of deficiency of spleen qi model pig with consumption gas relieving stagnant Qi model traditional on the traditional Chinese medical science and reserpine model, every sign of observing modeling front and back and nature convalescence pig body changes, and comprises body temperature, body weight, appetite, spirit, hair etc.Draw blood simultaneously and cut open the inspection pig only, measure before and after the modeling and the nature every organ index of convalescence pig body and with the variation of Ia index.
Test 1: the pig feed Chinese medicine additive is to the influence of the empty pork pig blood parameters of temper and immune index of correlation.
1, materials and methods
1.1 experimental animal
30 of DLY ternary commodity pork pigs about 10 kilograms after transition raised for 1 week after buying back, carry out group experiment.
1.2 trial drug
Press the pig feed Chinese medicine additive of the present invention of embodiment 1 prescription four preparations.The high, medium and low dosage group of pig feed Chinese medicine additive is in 0.25%, 0.5% and 1.0% ratio spice.
1.3 test grouping and processing
Pig is bought the back transition and raises a week, about 30 10 kilograms DLY piglets is divided into 5 groups at random is respectively normal control group, the empty control group of temper, the high, medium and low dosage group of pig feed Chinese medicine additive group, 6 every group.Except normal control group musculi colli every day injecting normal saline, other each group is all by 0.05mg/kg dosage musculi colli injection reserpine, once a day, modeling 12d, after modeling finishes, the empty control group of temper recovers 15d naturally, and the high, medium and low dosage group of pig feed Chinese medicine additive is in 0.25%, 0.5% and 1.0% ratio spice, and 15d feeds.
1.4 the collection of blood sample and preservation
After the off-test, the Baoding of lying on the back, vena cava anterior blood sampling 10mL gets the mensuration that the 2mL heparin anti-coagulating is used for lymphocyte subgroup, after all the other are placed to and separate out serum, 3000r the centrifugal 10min of min-1, collect blood serum sample, be sub-packed in the Eppendorf pipe, in-20 ℃ of preservations, to be measured.
1.5 the detection method of blood parameters and immune index of correlation
Measure blood parameters according to specification with SYSMEX-1800i type automatic clinical chemistry analyzer, the mensuration of cAMP, cGMP, cortisol adopts radioimmunology, wherein available from General Hospital of Beijing Military Command, tester is the SN-682 radio-immunity r-counter that Shanghai nuclear good fortune photoelectric instrument Co., Ltd produces to the cortisol kit available from Isotope Research institute of China Atomic Energy Science Research Institute, cAMP/cGMP kit; The EPICS-XL fluidic cell detector that t lymphocyte subset group's mensuration adopts U.S. Colter company to produce; The L-P method is adopted in the determination of activity of lactic dehydrogenase (LDH), and kit is provided by Shanghai Rongsheng Bioisystech Co., Ltd, and tester is that last Hisoon reaches the XD-811L semi-automatic biochemical analyzer that Medical Instruments company produces.
1.6 data analysis
Use the SPSS13.0 statistical analysis software that data are carried out the one-factor analysis of variance and correlation analysis, carry out multiple ratio again.
2, result and analysis
The testing result (n=6) of respectively organizing the test pig blood parameters by table 1 as can be known, after reserpine intramuscular injection pork pig success modeling, glutamic-oxalacetic transaminease (AST), glutamic-pyruvic transaminase (ALT), alkaline phosphatase (ALP), glutamic-pyruvic transaminase (GGT), uric acid (UA), creatine kinase (CK) all raise, wherein AST, ALP, GGT, CK rising remarkable (P<0.05); Total protein (TP), creatinine (CREA), albumin (ALB), serum glucose (GLU), triacylglycerol (TG), T-CHOL (CHO), lactic dehydrogenase (LDH), calcium (Ca), phosphorus (P), chlorine (Cl) all descend significantly (P<0.05).After the empty test pig of temper was treated through pig feed Chinese medicine additive various dose, AST, ALT, ALP, GGT, UA, CK compared reduction with the empty control group of temper, but still are higher than the normal control group; TP, CREA, ALB, GLU, TG, CHO, LDH, Ca, P, Cl raise to some extent, but do not return to original level, and wherein the middle and high dosage group of pig feed Chinese medicine additive effect is more obvious.Illustrate after the empty test pig of temper is through the treatment of pig feed Chinese medicine additive and recover normal substantially, every index is compared with the empty control group of temper all better recovery.
The testing result (n=6) of respectively organizing test pig immunity index of correlation by table 2 as can be known, after reserpine intramuscular injection pork pig success modeling, remarkable rising all appears in T cell subsets such as CD3, CD4, CD8, obviously reducing appears in cAMP.After the pig feed Chinese medicine additive treatment through various dose, T cell subsets such as CD3, CD4, CD8 all return to normal level, and the cAMP level significantly rises.
Wherein the middle and high dosage group of pig feed Chinese medicine additive effect is the most obvious, prompting, and the middle and high dosage group of pig feed Chinese medicine additive has remarkable result aspect the enhancing syndrome of deficiency of spleen qi model pig immunologic function.
Table 1
Table 2
3 conclusions
By above test, can see that pig syndrome of deficiency of spleen qi model can copy well, the middle and high dosage group of pig feed Chinese medicine additive can obviously be improved the clinical symptoms of syndrome of deficiency of spleen qi pig, strengthens its immunocompetence, promotes every blood parameters.
Test 2: the pig feed Chinese medicine additive is to the clinical treatment effect of syndrome of deficiency of spleen qi pork pig
In the dialectical principle of three large-scale pig farms according to syndrome of deficiency of spleen qi; select 90 syndrome of deficiency of spleen qi child care pigs to carry out the test of pig feed Chinese medicine additive result for the treatment of; with the positive contrast of shenling baizhu powder; observe the body surface changing features for the treatment of front and back piglet; statistics daily feed intake and daily gain, the variation of special survey diarrhea rate.Understand the pig feed Chinese medicine additive to the result for the treatment of of syndrome of deficiency of spleen qi child care pig.
1 material and method
1.1 pig feed Chinese medicine additive
Press the pig feed Chinese medicine additive of the present invention of embodiment 1 prescription four preparations.
1.2 selection and the grouping of test piglet
From the wean of 3 large-scale pig farms, Fujian Province, 21 ages in days and in 233 weak piglets that transition in 3 days is fed, standard according to syndrome of deficiency of spleen qi, One's spirits are drooping with the pig body, poor appetite, rush down down the loose stool, the not flourish grade of hair is principle, 90 DLY ablactation of picking syndrome of deficiency of spleen qi piglet, be divided into 5 groups at random, every group on each 1 hurdle, pig farm, 3 hurdles altogether, be respectively negative control group, positive controls, the pig feed Chinese medicine additive is low, in, a Senior Three test group, the negative control group basal feed of feeding wherein, the pig feed Chinese medicine additive is low, in, high dose group adds 0.25% respectively in basal diet, 0.5% and 1.0% pig feed Chinese medicine additive.Experimental period is 15d.Wherein positive controls is shenling baizhu powder, adds with 0.5% on basal diet.Prescription is: Radix Codonopsis 45g, bighead atractylodes rhizome 45g, Poria cocos 45g, honey-fried licorice root 45g, Chinese yam 45g, French beans 60g, Semen Nelumbinis 30g, balloonflower root 30g, coix seed 30g, fructus amomi 30g.
1.3 test diet and feeding and management
The experimental basis diet is formed and nutritional labeling sees Table 3, and raising and immune rules are carried out feeding and management routinely.
Table 3
1.3 index determining
In the weight that takes by weighing every test piglet the 1st day, the 15th day 7: 30 morning on an empty stomach that test formally begins, record every group of each stage consumption forage volume respectively, calculate daily gain, daily ingestion amount and material anharmonic ratio (F/G), diarrhea rate.
Daily gain=(weight-initial weight during off-test)/test fate
Material anharmonic ratio=duration of test is tested total feed consumption rate/duration of test total augment weight weight
Diarrhea rate=(a diarrhoea number * diarrhoea fate)/(testing total number of total fate * piglet) * 100%
2 results and analysis
The pig feed Chinese medicine additive the results are shown in Table 4(n=6 to the influence of insufficiency of the spleen weak piglet growth performance).As shown in Table 4, compare with the blank group, the pig feed Chinese medicine additive group daily gain of three dosage increases, and with the increase of dosage the highest trend is arranged; The material anharmonic ratio has reduction trend, and diarrhea rate is reduction trend, and significant difference (P<0.05), and The above results shows that the pig feed Chinese medicine additive can improve insufficiency of the spleen weak piglet daily gain, reduces material anharmonic ratio and diarrhea rate.Particularly high, middle dosage group has the significant symptom of improving the syndrome of deficiency of spleen qi piglet, reduces the effect of diarrhea rate.
Table 4
Group |
All the time (kg) |
End heavy (kg) |
Daily gain (g) |
The material anharmonic ratio |
Diarrhea rate (%) |
Control group |
6.38±0.66 |
8.43±1.75a |
137.67±65.83a |
2.02±0.33a |
43.43±8.98a |
Positive controls |
6.25±0.55 |
9.39±1.33b |
207.66±60.843b |
1.41±0.35b |
5.32±1.32c |
Low dose group |
6.24±0.53 |
9.19±1.33b |
196.67±57.742b |
1.58±0.44b |
10.36±4.46c |
Middle dosage group |
6.31±0.67 |
9.68±1.61b |
224.67±75.36b |
1.37±0.32b |
4.48±0.53c |
High dose group |
6.27±0.75 |
9.75±1.56b |
232.00±62.32b |
1.37±0.26b |
4.79±0.47c |
3 conclusions
Can see by test, the pig feed Chinese medicine additive can improve the symptom of diarrhea of clinical syndrome of deficiency of spleen qi child care pig well, increase daily gain, reduce the material anharmonic ratio, the middle and high dosage group of pig feed Chinese medicine additive particularly, more remarkable effect, with the classical prescription shenling baizhu powder that is used for syndrome of deficiency of spleen qi relatively, have the effect of better weightening finish and minimizing diarrhea rate, but do not have the difference on the statistics.Shenling baizhu powder is asthenic symptoms class non-prescribed medicine medicine.Prescription is to add Chinese yam, lotus seeds, Semen Lablab Album, coix seed, fructus amomi, balloonflower root to form on the Sijunzi Tang basis.
Test 3: the pig feed Chinese medicine additive is to the influence of weanling pig antioxidative functional
Choose parity close (3-5 tire), Du-length that age in days is identical-big suckling pig 12 nests (every nest 8-10 head), be divided into 4 groups at random, be respectively pig feed Chinese medicine additive senior middle school low dose group, blank group, every group of 4 nests.7 ages in days begin food calling test diet, until wean back 28 ages in days.From every nest, choose the close piglet 4-5 head of body weight during the wean of 25 ages in days, merge in the group, jump, every group each 18.Wherein the basic, normal, high dosage group of pig feed Chinese medicine additive in basal diet respectively the amount by 0.5%, 1.0% and 1.5% add the pig feed Chinese medicine additive respectively; The blank group basal diet of only feeding.
When weaned piglet 28d, every group is selected 6 near the piglet of average weight, the vena cava anterior of lying on the back blood sampling detects glutathione peroxidase (GSH-Px) activity, MDA (MDA) content, superoxide dismutase (SOD) content, catalase (CAT) content and TAC (T-AOC).
1, material and method
1.1 trial drug
Press the pig feed Chinese medicine additive of the present invention of embodiment 1 prescription four preparations.
1.2 experimental animal grouping and processing
Choose parity close (3-5 tire), Du-length that age in days is identical-big suckling pig 16 nests (every nest 8-10 head), be divided into 4 groups at random, be respectively pig feed Chinese medicine additive senior middle school low dose group, every group of 4 nests of blank group.7 ages in days begin food calling test diet, until wean back 28 ages in days.From every nest, choose the close piglet 4-5 head of body weight during the wean of 25 ages in days, merge in the group, jump, every group each 18.Wherein pig feed Chinese medicine additive group amount by 2.5kg/T, 5.0kg/T, 10kg/T in basal diet is added the pig feed Chinese medicine additive respectively; The blank group basal diet of only feeding; Each assembly is than all being made as graininess test diet after adding.
1.3 the collection of blood sample and processing
When weaned piglet 28d, every group is selected 6 near the piglet of average weight, and the vena cava anterior of lying on the back blood sampling 1-2mL adds liquaemin (25u/mL) anti-freezing, be placed to separate out serum after, the centrifugal 10min of 3000r/min collects serum, be sub-packed in the eppendorf pipe ,-20 ℃ of preservations, to be measured.
1.4 anti-oxidant index
Active two sulfo-dinitrobenzoic acid methods, MDA (MDA) content of adopting of glutathione peroxidase (GSH-Px) adopts sulfo-barbital method, TAC (T-AOC) to adopt chemical colorimetry, and tester is the VIS-7220 type visible spectrophotometer that Shanghai Techcomp Instrument Ltd. produces.
1.5 data analysis: use the SPSS11.5 statistical processing software and carry out one-way analysis of variance and DUNCAN multiple ratio.
2, result and analysis
2.1 the dynamic change of weanling pig serum T-AOC the results are shown in Table 5 pig feed Chinese medicine additives to weanling pig serum T-AOC(U mL-1) influence (n=6).
2.2 the dynamic change to weanling pig serum GSH-Px activity the results are shown in Table 6, the pig feed Chinese medicine additive is to the influence (n=6) of weanling pig serum GSH-Px activity.
2.3 the result of variations of weanling pig activity of SOD in serum sees Table 7, the pig feed Chinese medicine additive is to the influence (n=6) of weanling pig activity of SOD in serum.
2.4 the result of variations of weanling pig change of serum C AT activity sees Table 8, the pig feed Chinese medicine additive is to the influence (n=6) of weanling pig change of serum C AT activity.
2.5 the result of variations of weanling pig Content of MDA sees Table 9, the pig feed Chinese medicine additive is to the influence (n=6) of weanling pig Content of MDA.
Table 5
Table 6
Table 7
Table 8
Table 9
3 conclusions
The above results shows: the back 1d that weans compares all reductions before respectively organizing piglet serum TAC (T-AOC), hepatocuprein (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) activity and wean, and MDA content raises than wean is preceding; During the whole test, pig feed Chinese medicine additive height, middle dosage group serum T-AOC, SOD, GSH-Px, than low dose group and control group height, MDA content is lower than low dose group and control group to the CAT activity all the time all the time.The above results shows that pig feed Chinese medicine additive height, middle dosage can improve the activity of piglet body radicals scavenging enzyme, strengthens the oxidation resistance of body.
Four, the pig feed Chinese medicine additive is to the weanling pig Immune Effects
Choose parity close (3-5 tire), Du-length that age in days is identical-big suckling pig 12 nests (every nest 8-10 head), be divided into 4 groups at random, be respectively pig feed Chinese medicine additive senior middle school low dose group, blank group, every group of 4 nests.7 ages in days begin food calling test diet, until wean back 28 ages in days.From every nest, choose the close piglet 4-5 head of body weight during the wean of 25 ages in days, merge in the group, jump, every group each 18.Wherein the basic, normal, high dosage group of pig feed Chinese medicine additive in basal diet respectively the amount by 0.5%, 1.0% and 1.5% add the pig feed Chinese medicine additive respectively; The blank group basal diet of only feeding.
Detect piglet blood red blood cell number, leukocyte count, lymphocyte percentage, the content of serum immune globulin, complement is inquired into the pig feed Chinese medicine additive to the weanling pig Immune Effects.
1, materials and methods
1.1 press the pig feed Chinese medicine additive of the present invention of embodiment 1 prescription four preparations.
1.2 experimental animal grouping and the same test 3 of processing.
1.3 assay method and testing index
1.3.1 the mensuration of hematology project: when weaned piglet 14,28d, every group is selected 6 near the piglet of average weight, the vena cava anterior of lying on the back blood sampling 1mL, add the EDTA-K2 anti-freezing, detect red blood cell number (RBC) with SysmexK-1000 blood cell automatic analyzer, hemoglobin (HGB), hematid specific volume (HCT), mean corpuscular volume (MCV) (MCV), mean corpusular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) (MCHC), leukocyte count (WBC), lymphocyte percentage (LYM).
1.3.2 the mensuration of amynologic index: 3d, wean back 1,4,7,14,28d 7:30-9:30 in the morning before weaned piglet, 6 empty stomaches of every group of picked at random piglet (the pig unanimity of omnidistance blood sampling), the Baoding of lying on the back, vena cava anterior blood sampling, the conventional serum for preparing.Serum immune globulin (IgG, IgM, IgA), complement (C3, C4) are used immune turbidimetry, and kit is provided by Zhejiang Province Yilikang Bioisystech Co., Ltd.Tester is that last Hisoon reaches the XD-811L semi-automatic biochemical analyzer that Medical Instruments company produces.
1.4 data analysis: use the SPSS11.5 statistical processing software and carry out one-way analysis of variance and Duncan multiple ratio.
2, result and analysis
2.1 the variation of weanling pig 14,28d blood project such as table 10, table 11, table 10 is back 14 days measurement results (n=6) for examination pig blood project of wean, and table 11 weanling pig 28 ages in days are for examination pig blood project measurement result (n=6)
2.2 the weanling pig serum immunoglobulin IgG, content
Table 12 is that the pig feed Chinese medicine additive is to the influence (n=6 of weanling pig serum immunoglobulin IgG content; G/L).
Table 10
Table 11
Table 12
3 conclusions
The above results shows: the pig feed Chinese medicine additive can improve weanling pig blood rbc number, leukocyte count, lymphocyte percentage, can promote the generation of weanling pig serum immune globulin, complement, show that the pig feed Chinese medicine additive is to the immunological enhancement of weanling pig.
The result of the test of pig feed Chinese medicine additive with prescription 1-4 is similar, and result of the test shows:
1, by above test, can see that pig syndrome of deficiency of spleen qi model can copy well, the middle and high dosage group of pig feed Chinese medicine additive can obviously be improved the clinical symptoms of syndrome of deficiency of spleen qi pig, strengthens its immunocompetence, promotes every blood parameters.
2, the pig feed Chinese medicine additive can improve the symptom of diarrhea of clinical syndrome of deficiency of spleen qi child care pig well, increase daily gain, reduce the material anharmonic ratio, the middle and high dosage group of pig feed Chinese medicine additive particularly, more remarkable effect, compare with the classical prescription shenling baizhu powder that is used for syndrome of deficiency of spleen qi, have the effect of better weightening finish and minimizing diarrhea rate, but do not have the difference on the statistics.
3, pig feed Chinese medicine additive height, middle dosage can improve the activity of piglet body radicals scavenging enzyme, strengthen the oxidation resistance of body.
4, the pig feed Chinese medicine additive can improve weanling pig blood rbc number, leukocyte count, lymphocyte percentage, can promote the weanling pig serum immune globulin to show that the pig feed Chinese medicine additive is to the immunological enhancement of weanling pig.
More than comprehensive, can draw to draw a conclusion: the reserpine model is the temper chronic diseases marked by deficiency of vital energy model of suitable pig; The pig feed Chinese medicine additive adds with 0.5% and 1.0%, all can effectively improve the clinical symptoms of syndrome of deficiency of spleen qi child care pig and reduce diarrhea rate, can improve the daily gain of syndrome of deficiency of spleen qi child care pig simultaneously, reduces the material anharmonic ratio.Raise pigs and use the pig feed Chinese medicine additive to add with 0.5% and 1.0% clinically to have the effect for the treatment of clinical syndrome of deficiency of spleen qi pig preferably, can effectively improve oxidation resistance and the immunologic function of pork pig simultaneously.Consider result of use and price factor, the pig feed Chinese medicine additive recommends the dosage with 0.5% to carry out the feed interpolation.
The above only is the several of the numerous by way of example of the present invention; be not so limit claim of the present invention; every equivalent structure or equivalent flow process conversion that utilizes description of the present invention to do; or directly or indirectly be used in other relevant technical fields, all in like manner be included in the scope of patent protection of the present invention.