CN104489420A - Pig feed additive capable of booting immunity and improving meat quality - Google Patents
Pig feed additive capable of booting immunity and improving meat quality Download PDFInfo
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Abstract
The invention discloses a pig feed additive capable of booting immunity and improving meat quality, and belongs to the field of feed additives. The pig feed additive is composed of Chinese herbal medicine, plant extract, amino acid, mineral substances, vitamins and the like. The pig feed additive is scientific in medicine compatibility, capable of booting the immunity of pigs and decreasing the morbidity of pigs, and beneficial for reducing the usage of antibiotics; meanwhile, the additive can improve the meat quality of pigs, improve the breeding effect and increase economic benefits.
Description
Technical field
The present invention relates to a kind of pig's feed additive, especially relate to a kind of develop immunitypty and the pig's feed additive improving meat, belong to feed additive field.
Background technology
Along with the flourish of China's aquaculture and the attention rate of people to Pork Security are more and more higher, research and develop pollution-free, noresidue, hot issue that green safety, the feed addictive that do not develop immunity to drugs have become research, be also the trend of future in feed industry development.At present, the additive used in feedstuff industry is still main based on chemical synthesis, these additives more or less can be with and serve side effect, remain in pig body or some bacterium in pig body is developed immunity to drugs, bring harm in various degree eventually through food chain to human body, therefore develop and to substitute or the material of Some substitute chemical addition agent seems particularly important.Chinese herbal medicine is as the rarity of China, and the utilization in feed is with a long history, also has unique advantage compared with chemical synthesis additive.Resources of Chinese medicinal herb in natural plants, medicament residue can not be produced, bacterium can not be caused, virus variation, feature free from environmental pollution etc., can play and substitute or the effect of Some substitute medicated premix, antibiotic etc.
Pork is the maximum meat product of China's consumption figure, very popular.Along with the fast development of aquaculture and improving constantly of living standard, people to the demand of meat products from amount to fundamental change.Therefore, how to provide best in quality to people, local flavor be delicious, safety, nutrition meat products be the direction of animal husbandry future development.Meat quality comprises the organoleptic attribute, technical indicator, nutritive value, sanitary condition etc. of meat.Affect a lot of because have of meat quality, as inherent cause, feed factor and feeding process management etc., wherein feeding quality affects the very important factor of of meat quality.
Therefore, the pig's feed additive by using that the green feed development of raw materials such as Chinese herbal medicine goes out a kind of safe, efficient, nutrition, health has develop immunitypty and improving meat seems particularly important.Not only can strengthen pig immunity, reduce the use of the chemical synthesis class medicines such as antibiotic, open and can improve meat quality, improve pig flesh flavor, be conducive to reducing cultivation risk, increase culture benefit, be conducive to promoting feed and the healthy and orderly development of livestock, to raising culture benefit, reduce cultivation risk significant.
Summary of the invention
The object of the present invention is to provide a kind of develop immunitypty and the pig's feed additive improving meat.This additive can strengthen the immunity of pig, and reduce the incidence of disease of pig, be conducive to reducing antibiotic use, this additive can also improve meat quality simultaneously, promotes culture efficiency, increases economic benefit.
Technical scheme provided by the invention is as follows:
Develop immunitypty and the pig's feed additive improving meat, every 100 parts are made up of following raw materials by weight portion: rhizoma nardostachyos 3-5 part, Japanese polygala 3-5 part, Rabdosia rubescens 2-4 part, Sculellaria barbata 2-4 part, rush 2-3 part, red stilbene 2-3 part, storax 2-3 part, cloves 1-3 part, fennel seeds 1-3 part, carpet bugle 1-2 part, refining accumulated snow grass meal 2-4 part, Caulis Polygoni Multiflori extract 2-3 part, L-Trp 0.1-0.3 part, L-Leu 0.1-0.3 part, glutamic acid 0.1-0.2 part, valine 0.1-0.2 part, Cobastab
10.05-0.1 part, Cobastab
120.05-0.1 part, vitamin D
30.05-0.1 part, vitamin E 0.1-0.3 part, VBT 0.2-0.3 part, betaine 0.2-0.3 part, ferrous citrate 0.3-0.5 part, dipotassium hydrogen phosphate 0.1-0.2 part, magnesium chloride 0.1-0.2 part, manganese chloride 0.1-0.2 part, zinc acetate 0.3-0.6 part, phytase 0.05-0.2 part, amylase 0.05-0.1 part, zytase 0.1-0.2 part, alpha-galactosidase 0.01-0.05 part, butylated hydroxy anisole 0.02-0.05 part, citric acid 0.03-0.06 part, probiotics 0.5-1 part, all the other are carrier, carrier is peanut hull meal and defatted rice bran 1:2-3 composition in mass ratio, 40 mesh sieves pulverized in advance by peanut hull meal and defatted rice bran.
The preparation method of described refining accumulated snow grass meal is:
(1) cleaned by fresh centella blade, chopping, be placed in drying box and dry, make its moisture lower than 15%, bake out temperature is 60-70 DEG C, pulverizes, and crosses 60 mesh sieves, obtains accumulated snow grass meal;
(2) by accumulated snow grass meal and sodium chloride 100:(0.5-1 by weight) mix, then by solid-liquid ratio 1:(20-30) add distilled water, heating, make temperature reach 95-98 DEG C, lixiviate 0.5-1h, is reduced to 50-60 DEG C by temperature afterwards, lixiviate 2-4h, filters, gets filtrate;
(3) filtrate is concentrated, vacuum drying, pulverized 60 mesh sieves, obtain refining accumulated snow grass meal;
The preparation method of described Caulis Polygoni Multiflori extract is:
(1) vine of multiflower knotweed of drying is pulverized, cross 40 mesh sieves, with cellulase, sodium chloride and magnesium chloride by weight 100:(0.1-0.3): (0.5-1): (0.2-0.4) mixes, then by solid-liquid ratio 1:(10-15) add distilled water, at 80-90 DEG C of temperature, soak 1-2h, then at 40-50 DEG C of temperature, 2-4h is soaked, soak complete filtration afterwards, get filter residue, dry, make its moisture lower than 15%, bake out temperature is 55-65 DEG C;
(2) by the ethanol of filter residue and the 70-75% after drying by solid-liquid ratio 1:(10-20) mix, be placed in supersonic extractors and extract, get filtrate, extract 2 times, merge 2 filtrates;
(3) filtrate after merging loaded in container, water-bath container being placed in 85-90 DEG C removes ethanol, then by filtrate concentrate drying, pulverizes 60 mesh sieves, and obtains Caulis Polygoni Multiflori extract;
Described probiotics by bacillus subtilis, Pediococcus acidilactici, VREF, Rhodopseudomonas palustris by bacteria suspension volume ratio 1:(1-3): (1-3): (1-2) mixes; Wherein, bacillus subtilis bacteria suspension concentration is 2.5 × 10
8individual/ml, Pediococcus acidilactici bacteria suspension concentration is 1.6 × 10
6individual/ml, VREF bacteria suspension concentration is 2.2 × 10
7individual/ml, Rhodopseudomonas palustris bacteria suspension concentration is 2.8 × 10
8individual/ml;
Develop immunitypty and the pig's feed additive improving meat, the addition in pig feed is 100-300g/t.
Beneficial effect
A kind of develop immunitypty provided by the invention is formed by Multiple components scientific compatibility with the pig's feed additive improving meat, has develop immunitypty and the effect improving meat.By the extraction to centella fleece-flower root rattan active ingredient, enhance effect of the present invention, the effect making the present invention increase immunity is further promoted.By the interpolation of probiotics, facilitate intestinal growth and immune function, Chinese herbal medicine can be made better to play the double action of medicine and nutrition.In process of the test, also find that the present invention is except the effect with develop immunitypty, also can promote that pig grows, and improves meat quality, substantially increases pig farm economic benefit.
Specific embodiment
The present invention is further described below by way of specific embodiment.
Embodiment 1
Develop immunitypty and the pig's feed additive improving meat, every 100 parts are made up of following raw materials by weight portion: rhizoma nardostachyos 3 parts, Japanese polygala 3 parts, Rabdosia rubescens 2 parts, Sculellaria barbata 2 parts, rush 2 parts, red stilbene 2 parts, storax 2 parts, cloves 1 part, fennel seeds 1 part, carpet bugle 1 part, refining accumulated snow grass meal 2 parts, Caulis Polygoni Multiflori extract 2 parts, L-Trp 0.1 part, L-Leu 0.1 part, 0.1 part, glutamic acid, valine 0.1 part, Cobastab
10.05 part, Cobastab
120.05 part, vitamin D
30.05 part, vitamin E 0.1 part, VBT 0.2 part, betaine 0.2 part, ferrous citrate 0.3 part, dipotassium hydrogen phosphate 0.1 part, 0.1 part, magnesium chloride, manganese chloride 0.1 part, zinc acetate 0.3 part, phytase 0.05 part, amylase 0.05 part, zytase 0.1 part, alpha-galactosidase 0.01 part, butylated hydroxy anisole 0.02 part, citric acid 0.03 part, probiotics 0.5 part, all the other are carrier, carrier is peanut hull meal and defatted rice bran 1:2 composition in mass ratio, and 40 mesh sieves pulverized in advance by peanut hull meal and defatted rice bran.
The preparation method of described refining accumulated snow grass meal is:
(1) cleaned by fresh centella blade, chopping, be placed in drying box and dry, make its moisture lower than 15%, bake out temperature is 60 DEG C, pulverizes, and crosses 60 mesh sieves, obtains accumulated snow grass meal;
(2) mixed by weight 100:0.5 with sodium chloride by accumulated snow grass meal, then add distilled water by solid-liquid ratio 1:20, heating, makes temperature reach 95 DEG C, lixiviate 0.5h, afterwards temperature is reduced to 50 DEG C, lixiviate 2h, filters, gets filtrate;
(3) filtrate is concentrated, vacuum drying, pulverized 60 mesh sieves, obtain refining accumulated snow grass meal;
The preparation method of described Caulis Polygoni Multiflori extract is:
(1) vine of multiflower knotweed of drying is pulverized, cross 40 mesh sieves, mix by weight 100:0.1:0.5:0.2 with cellulase, sodium chloride and magnesium chloride, then add distilled water by solid-liquid ratio 1:10, at 80 DEG C of temperature, soak 1h, then at 40 DEG C of temperature, 2h is soaked, soak complete filtration afterwards, get filter residue, dry, make its moisture lower than 15%, bake out temperature is 55 DEG C;
(2) by dry after filter residue with 70% ethanol mix by solid-liquid ratio 1:10, be placed in supersonic extractors and extract, get filtrate, extract 2 times, merge 2 filtrates;
(3) filtrate after merging loaded in container, water-bath container being placed in 85 DEG C removes ethanol, then by filtrate concentrate drying, pulverizes 60 mesh sieves, and obtains Caulis Polygoni Multiflori extract;
Described probiotics is mixed by bacteria suspension volume ratio 1:1:1:1 by bacillus subtilis, Pediococcus acidilactici, VREF, Rhodopseudomonas palustris; Wherein, bacillus subtilis bacteria suspension concentration is 2.5 × 10
8individual/ml, Pediococcus acidilactici bacteria suspension concentration is 1.6 × 10
6individual/ml, VREF bacteria suspension concentration is 2.2 × 10
7individual/ml, Rhodopseudomonas palustris bacteria suspension concentration is 2.8 × 10
8individual/ml;
Develop immunitypty and the pig's feed additive improving meat, the addition in pig feed is 100g/t.
Embodiment 2
Develop immunitypty and the pig's feed additive improving meat, every 100 parts are made up of following raw materials by weight portion: rhizoma nardostachyos 5 parts, Japanese polygala 5 parts, Rabdosia rubescens 4 parts, Sculellaria barbata 4 parts, rush 3 parts, red stilbene 3 parts, storax 3 parts, cloves 3 parts, fennel seeds 3 parts, carpet bugle 2 parts, refining accumulated snow grass meal 4 parts, Caulis Polygoni Multiflori extract 3 parts, L-Trp 0.3 part, L-Leu 0.3 part, 0.2 part, glutamic acid, valine 0.2 part, Cobastab
10.1 part, Cobastab
120.1 part, vitamin D
30.1 part, vitamin E 0.3 part, VBT 0.3 part, betaine 0.3 part, ferrous citrate 0.5 part, dipotassium hydrogen phosphate 0.2 part, 0.2 part, magnesium chloride, manganese chloride 0.2 part, zinc acetate 0.6 part, phytase 0.2 part, amylase 0.1 part, zytase 0.2 part, alpha-galactosidase 0.05 part, butylated hydroxy anisole 0.05 part, citric acid 0.06 part, probiotics 1 part, all the other are carrier, carrier is peanut hull meal and defatted rice bran 1:3 composition in mass ratio, and 40 mesh sieves pulverized in advance by peanut hull meal and defatted rice bran.
The preparation method of described refining accumulated snow grass meal is:
(1) cleaned by fresh centella blade, chopping, be placed in drying box and dry, make its moisture lower than 15%, bake out temperature is 70 DEG C, pulverizes, and crosses 60 mesh sieves, obtains accumulated snow grass meal;
(2) mixed by weight 100:1 with sodium chloride by accumulated snow grass meal, then add distilled water by solid-liquid ratio 1:30, heating, makes temperature reach 98 DEG C, lixiviate 1h, afterwards temperature is reduced to 60 DEG C, lixiviate 4h, filters, gets filtrate;
(3) filtrate is concentrated, vacuum drying, pulverized 60 mesh sieves, obtain refining accumulated snow grass meal;
The preparation method of described Caulis Polygoni Multiflori extract is:
(1) vine of multiflower knotweed of drying is pulverized, cross 40 mesh sieves, mix by weight 100:0.3:1:0.4 with cellulase, sodium chloride and magnesium chloride, then add distilled water by solid-liquid ratio 1:15, at 90 DEG C of temperature, soak 2h, then at 50 DEG C of temperature, 4h is soaked, soak complete filtration afterwards, get filter residue, dry, make its moisture lower than 15%, bake out temperature is 65 DEG C;
(2) by dry after filter residue with 75% ethanol mix by solid-liquid ratio 1:20, be placed in supersonic extractors and extract, get filtrate, extract 2 times, merge 2 filtrates;
(3) filtrate after merging loaded in container, water-bath container being placed in 90 DEG C removes ethanol, then by filtrate concentrate drying, pulverizes 60 mesh sieves, and obtains Caulis Polygoni Multiflori extract;
Described probiotics is mixed by bacteria suspension volume ratio 1:3:3:2 by bacillus subtilis, Pediococcus acidilactici, VREF, Rhodopseudomonas palustris; Wherein, bacillus subtilis bacteria suspension concentration is 2.5 × 10
8individual/ml, Pediococcus acidilactici bacteria suspension concentration is 1.6 × 10
6individual/ml, VREF bacteria suspension concentration is 2.2 × 10
7individual/ml, Rhodopseudomonas palustris bacteria suspension concentration is 2.8 × 10
8individual/ml;
Develop immunitypty and the pig's feed additive improving meat, the addition in pig feed is 300g/t.
Embodiment 3
Develop immunitypty and the pig's feed additive improving meat, every 100 parts are made up of following raw materials by weight portion: rhizoma nardostachyos 4 parts, Japanese polygala 4 parts, Rabdosia rubescens 3 parts, Sculellaria barbata 3 parts, rush 2.5 parts, red stilbene 2.5 parts, storax 2.5 parts, cloves 2 parts, fennel seeds 2 parts, carpet bugle 1.5 parts, refining accumulated snow grass meal 3 parts, Caulis Polygoni Multiflori extract 2.5 parts, L-Trp 0.2 part, L-Leu 0.2 part, 0.15 part, glutamic acid, valine 0.15 part, Cobastab
10.08 part, Cobastab
120.08 part, vitamin D
30.08 part, vitamin E 0.2 part, VBT 0.25 part, betaine 0.25 part, ferrous citrate 0.4 part, dipotassium hydrogen phosphate 0.15 part, 0.15 part, magnesium chloride, manganese chloride 0.15 part, zinc acetate 0.5 part, phytase 0.1 part, amylase 0.06 part, zytase 0.15 part, alpha-galactosidase 0.03 part, butylated hydroxy anisole 0.04 part, citric acid 0.05 part, probiotics 0.8 part, all the other are carrier, carrier is peanut hull meal and defatted rice bran 1:2.5 composition in mass ratio, and 40 mesh sieves pulverized in advance by peanut hull meal and defatted rice bran.
The preparation method of described refining accumulated snow grass meal is:
(1) cleaned by fresh centella blade, chopping, be placed in drying box and dry, make its moisture lower than 15%, bake out temperature is 65 DEG C, pulverizes, and crosses 60 mesh sieves, obtains accumulated snow grass meal;
(2) mixed by weight 100:0.8 with sodium chloride by accumulated snow grass meal, then add distilled water by solid-liquid ratio 1:25, heating, makes temperature reach 96 DEG C, lixiviate 0.8h, afterwards temperature is reduced to 55 DEG C, lixiviate 3h, filters, gets filtrate;
(3) filtrate is concentrated, vacuum drying, pulverized 60 mesh sieves, obtain refining accumulated snow grass meal;
The preparation method of described Caulis Polygoni Multiflori extract is:
(1) vine of multiflower knotweed of drying is pulverized, cross 40 mesh sieves, mix by weight 100:0.2:0.6:0.3 with cellulase, sodium chloride and magnesium chloride, then add distilled water by solid-liquid ratio 1:13, at 85 DEG C of temperature, soak 1.5h, then at 45 DEG C of temperature, 3h is soaked, soak complete filtration afterwards, get filter residue, dry, make its moisture lower than 15%, bake out temperature is 60 DEG C;
(2) filter residue after oven dry is mixed by solid-liquid ratio 1:15 with the ethanol of 70-75%, be placed in supersonic extractors and extract, get filtrate, extract 2 times, merge 2 filtrates;
(3) filtrate after merging loaded in container, water-bath container being placed in 88 DEG C removes ethanol, then by filtrate concentrate drying, pulverizes 60 mesh sieves, and obtains Caulis Polygoni Multiflori extract;
Described probiotics is mixed by bacteria suspension volume ratio 1:2:2:1.5 by bacillus subtilis, Pediococcus acidilactici, VREF, Rhodopseudomonas palustris; Wherein, bacillus subtilis bacteria suspension concentration is 2.5 × 10
8individual/ml, Pediococcus acidilactici bacteria suspension concentration is 1.6 × 10
6individual/ml, VREF bacteria suspension concentration is 2.2 × 10
7individual/ml, Rhodopseudomonas palustris bacteria suspension concentration is 2.8 × 10
8individual/ml;
Develop immunitypty and the pig's feed additive improving meat, the addition in pig feed is 200g/t.
test example
1, experimental animal and experimental design
Test selection 200 subordinate plant of Qingyuan City Jiang Feng feed corporation,Ltd average weights are " DLY " tri-crossbreeding of about 30kg, be divided into 4 groups at random, often organize 5 repetitions, each repetition 10 pigs (male and female half and half), comprising a blank group and 3 test group.Blank group daily ration does not add any additive obtained in embodiment 1-3, all the other 3 test group swine rationses add the obtained additive of embodiment 1-3 respectively, live pig free choice feeding and drinking-water, carry out immunity and expelling parasite routinely, in experimental period, every day observes and records the mental status of pig diarrhoea, influenza and pig.Experimental period 90d.In feeding process, each group of pig incidence is added up.At the end of feeding experiment, the growth performance of pig, diarrhea rate and immune indexes are analyzed.
Data statistic analysis
Test data adopts SPSS19.0 statistical software to carry out statistical analysis, the results are shown in following table.
Table 1 growth performance index
| Project | Control group | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Daily ingestion amount/kg | 1.48 | 1.55 | 1.59 | 1.75 |
| Daily gain/kg | 0.52 | 0.57 | 0.62 | 0.70 |
| Feed-weight ratio | 2.85 | 2.72 | 2.56 | 2.50 |
Daily ingestion amount (ADFI): the feeding capacity of accurate recording each hurdle test pig and clout amount, calculates every pig average daily ingestion amount weekly and full phase average daily ingestion amount;
Daily gain (ADG): test pig starts weekly and terminates morning 7:30 and weighs on an empty stomach, calculates the average daily gain weekly of every pig and full phase average daily gain;
Feed-weight ratio: (F/G): calculate according to feed intake and weightening finish.
Table 2 immune indexes, diarrhea rate, the incidence of disease
| Project | Control group | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| IgA/(g/L) | 0.13 | 0.18 | 0.22 | 0.26 |
| IgG/(g/L) | 5.78 | 6.32 | 6.68 | 7.76 |
| IgM(g/L) | 0.89 | 0.96 | 0.99 | 1.03 |
| Diarrhea rate (%) | 13.0% | 7.2% | 6.3% | 2.8% |
| The incidence of disease (%) | 13.2% | 8.7% | 6.9% | 2.5% |
Immune indexes measures: the 7:30 in morning last day that experimental period terminates, and often group is chosen two and measured IgA, IgG and IgM level.
Diarrhea rate: diarrhea rate is experimental period diarrhoea piglet head/(test piglet head number × test number of days) × 100%.
The incidence of disease: sick pig generates heat suddenly and reaches 42 DEG C even higher, and ight soil is dry and hard, lassitude, anorexia or do not eat, normal accumbency together, is reluctant activity, expiratory dyspnea, cough, eye, nose stream mucus.
Table 3 meat quality index
| Project | Control group | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| The thickness of backfat (cm) | 1.38 | 1.32 | 1.26 | 1.23 |
| Lean meat percentage (%) | 71.4 | 73.6 | 74.7 | 76.5 |
| Dressing percentage (%) | 70.2 | 75.5 | 77.8 | 78.6 |
The raw material of this product is the material of green safety, pig body immunity can be strengthened, promote pig growth, improve meat quality, can be used as antibiotic substitute adds in pig feed, produce the mixed feed that disease resisting effect is good, promotion pig grows fast, there are good using value and wide market prospects.
Claims (8)
1. develop immunitypty and the pig's feed additive improving meat, is characterized in that every 100 parts are made up of following raw materials by weight portion: rhizoma nardostachyos 3-5 part, Japanese polygala 3-5 part, Rabdosia rubescens 2-4 part, Sculellaria barbata 2-4 part, rush 2-3 part, red stilbene 2-3 part, storax 2-3 part, cloves 1-3 part, fennel seeds 1-3 part, carpet bugle 1-2 part, refining accumulated snow grass meal 2-4 part, Caulis Polygoni Multiflori extract 2-3 part, L-Trp 0.1-0.3 part, L-Leu 0.1-0.3 part, glutamic acid 0.1-0.2 part, valine 0.1-0.2 part, Cobastab
10.05-0.1 part, Cobastab
120.05-0.1 part, vitamin D
30.05-0.1 part, vitamin E 0.1-0.3 part, VBT 0.2-0.3 part, betaine 0.2-0.3 part, ferrous citrate 0.3-0.5 part, dipotassium hydrogen phosphate 0.1-0.2 part, magnesium chloride 0.1-0.2 part, manganese chloride 0.1-0.2 part, zinc acetate 0.3-0.6 part, phytase 0.05-0.2 part, amylase 0.05-0.1 part, zytase 0.1-0.2 part, alpha-galactosidase 0.01-0.05 part, butylated hydroxy anisole 0.02-0.05 part, citric acid 0.03-0.06 part, probiotics 0.5-1 part, all the other are carrier, carrier is peanut hull meal and defatted rice bran 1:2-3 composition in mass ratio, 40 mesh sieves pulverized in advance by peanut hull meal and defatted rice bran,
Described probiotics by bacillus subtilis, Pediococcus acidilactici, VREF, Rhodopseudomonas palustris by bacteria suspension volume ratio 1:(1-3): (1-3): (1-2) mixes; Wherein, bacillus subtilis bacteria suspension concentration is 2.5 × 10
8individual/ml, Pediococcus acidilactici bacteria suspension concentration is 1.6 × 10
6individual/ml, VREF bacteria suspension concentration is 2.2 × 10
7individual/ml, Rhodopseudomonas palustris bacteria suspension concentration is 2.8 × 10
8individual/ml.
2. a kind of develop immunitypty and improve the pig's feed additive of meat according to claim 1, is characterized in that every 100 parts are made up of following raw materials by weight portion: rhizoma nardostachyos 4 parts, Japanese polygala 4 parts, Rabdosia rubescens 3 parts, Sculellaria barbata 3 parts, rush 2.5 parts, red stilbene 2.5 parts, storax 2.5 parts, cloves 2 parts, fennel seeds 2 parts, carpet bugle 1.5 parts, refining accumulated snow grass meal 3 parts, Caulis Polygoni Multiflori extract 2.5 parts, L-Trp 0.2 part, L-Leu 0.2 part, 0.15 part, glutamic acid, valine 0.15 part, Cobastab
10.08 part, Cobastab
120.08 part, vitamin D
30.08 part, vitamin E 0.2 part, VBT 0.25 part, betaine 0.25 part, ferrous citrate 0.4 part, dipotassium hydrogen phosphate 0.15 part, 0.15 part, magnesium chloride, manganese chloride 0.15 part, zinc acetate 0.5 part, phytase 0.1 part, amylase 0.06 part, zytase 0.15 part, alpha-galactosidase 0.03 part, butylated hydroxy anisole 0.04 part, citric acid 0.05 part, probiotics 0.8 part, all the other are carrier, carrier is peanut hull meal and defatted rice bran 1:2.5 composition in mass ratio, and 40 mesh sieves pulverized in advance by peanut hull meal and defatted rice bran;
Describedly to be mixed by bacteria suspension volume ratio 1:2:2:1.5 by bacillus subtilis, Pediococcus acidilactici, VREF, Rhodopseudomonas palustris; Wherein, bacillus subtilis bacteria suspension concentration is 2.5 × 10
8individual/ml, Pediococcus acidilactici bacteria suspension concentration is 1.6 × 10
6individual/ml, VREF bacteria suspension concentration is 2.2 × 10
7individual/ml, Rhodopseudomonas palustris bacteria suspension concentration is 2.8 × 10
8individual/ml.
3. a kind of develop immunitypty and the pig's feed additive improving meat according to claim 1-2, is characterized in that the preparation method of refining accumulated snow grass meal is:
(1) cleaned by fresh centella blade, chopping, be placed in drying box and dry, make its moisture lower than 15%, bake out temperature is 60-70 DEG C, pulverizes, and crosses 60 mesh sieves, obtains accumulated snow grass meal;
(2) by accumulated snow grass meal and sodium chloride 100:(0.5-1 by weight) mix, then by solid-liquid ratio 1:(20-30) add distilled water, heating, make temperature reach 95-98 DEG C, lixiviate 0.5-1h, is reduced to 50-60 DEG C by temperature afterwards, lixiviate 2-4h, filters, gets filtrate;
(3) filtrate is concentrated, vacuum drying, pulverized 60 mesh sieves, obtain refining accumulated snow grass meal.
4. a kind of develop immunitypty and the pig's feed additive improving meat according to claim 1-2, is characterized in that the preparation method of Caulis Polygoni Multiflori extract is:
(1) vine of multiflower knotweed of drying is pulverized, cross 40 mesh sieves, with cellulase, sodium chloride and magnesium chloride by weight 100:(0.1-0.3): (0.5-1): (0.2-0.4) mixes, then by solid-liquid ratio 1:(10-15) add distilled water, at 80-90 DEG C of temperature, soak 1-2h, then at 40-50 DEG C of temperature, 2-4h is soaked, soak complete filtration afterwards, get filter residue, dry, make its moisture lower than 15%, bake out temperature is 55-65 DEG C;
(2) by the ethanol of filter residue and the 70-75% after drying by solid-liquid ratio 1:(10-20) mix, be placed in supersonic extractors and extract, get filtrate, extract 2 times, merge 2 filtrates;
(3) filtrate after merging loaded in container, water-bath container being placed in 85-90 DEG C removes ethanol, then by filtrate concentrate drying, pulverizes 60 mesh sieves, and obtains Caulis Polygoni Multiflori extract.
5. a kind of develop immunitypty and improve the pig's feed additive of meat according to claim 3, is characterized in that the preparation method of refining accumulated snow grass meal is:
(1) cleaned by fresh centella blade, chopping, be placed in drying box and dry, make its moisture lower than 15%, bake out temperature is 65 DEG C, pulverizes, and crosses 60 mesh sieves, obtains accumulated snow grass meal;
(2) mixed by weight 100:0.8 with sodium chloride by accumulated snow grass meal, then add distilled water by solid-liquid ratio 1:25, heating, makes temperature reach 96 DEG C, lixiviate 0.8h, afterwards temperature is reduced to 55 DEG C, lixiviate 3h, filters, gets filtrate;
(3) filtrate is concentrated, vacuum drying, pulverized 60 mesh sieves, obtain refining accumulated snow grass meal.
6. a kind of develop immunitypty and improve the pig's feed additive of meat according to claim 4, is characterized in that the preparation method of Caulis Polygoni Multiflori extract is:
(1) vine of multiflower knotweed of drying is pulverized, cross 40 mesh sieves, mix by weight 100:0.2:0.6:0.3 with cellulase, sodium chloride and magnesium chloride, then add distilled water by solid-liquid ratio 1:13, at 85 DEG C of temperature, soak 1.5h, then at 45 DEG C of temperature, 3h is soaked, soak complete filtration afterwards, get filter residue, dry, make its moisture lower than 15%, bake out temperature is 60 DEG C;
(2) filter residue after oven dry is mixed by solid-liquid ratio 1:15 with the ethanol of 70-75%, be placed in supersonic extractors and extract, get filtrate, extract 2 times, merge 2 filtrates;
(3) filtrate after merging loaded in container, water-bath container being placed in 88 DEG C removes ethanol, then by filtrate concentrate drying, pulverizes 60 mesh sieves, and obtains Caulis Polygoni Multiflori extract.
7. a kind of develop immunitypty and the pig's feed additive improving meat according to claim 1-2, is characterized in that the addition in pig feed is 100-300g/t.
8. a kind of develop immunitypty and improve the pig's feed additive of meat according to claim 7, is characterized in that the addition in pig feed is 200g/t.
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