CN110100965A - A kind of derma type essential oil organic acid composite feed additive and its preparation method and application - Google Patents
A kind of derma type essential oil organic acid composite feed additive and its preparation method and application Download PDFInfo
- Publication number
- CN110100965A CN110100965A CN201910462347.0A CN201910462347A CN110100965A CN 110100965 A CN110100965 A CN 110100965A CN 201910462347 A CN201910462347 A CN 201910462347A CN 110100965 A CN110100965 A CN 110100965A
- Authority
- CN
- China
- Prior art keywords
- essential oil
- organic acid
- acid
- sodium
- feed additive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Birds (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of derma type essential oil organic acid composite feed additive and its preparation method and application, which is respectively " plants essential oil+organic acid " core particles, " casing+organic acid " middle layer coatings and crosses stomach outer layer coating layer from inside to outside.Plants essential oil and organic acid are combined by the present invention using derma technology, and pass through rational proportion, obtain a kind of novel coating type feed addictive, the additive can effectively pass through gastric acid, the slow release in animal intestinal tract, so that the effective component of product can reach animal intestinal tract, play physiology and immunization, Biochemical Indices In Serum can be improved simultaneously, improve Immune Organs of Body index, reduce gastrointestinal content pH value, control harmful microbe growth, keep the balance of intestinal microflora, improve small intestine Intestinal Morphology, increase intestine of young pigs mucous membrane permeability, protect gut barrier, improve the utilization rate of nutriment, to promote animal body to grow, improve production performance.
Description
Technical field
The present invention relates to a kind of animal feed additive, in particular to a kind of derma type essential oil organic acid mixed feed
Additive and its preparation method and application.
Background technique
Plants essential oil is a Plant Extracts, is the volatile liquid of fragranced, has food calling, growth promotion, antibacterial disappears
The effects of inflammation, resisting stress, disease preventing and treating, anti-feed mold；Organic acid refers to that it can pass through with acid organic compound
The acid ion or hydrogen ion dissociateed enters bacterial cell membrane, increases osmotic pressure intracellular, reduces interior environment acid value, makes bacterium
Eubolism gets muddled, or even cracking is dead, to reduce the quantity of harmful bacteria indirectly.Although at present to plants essential oil and
Organic acid has certain research, but compatibility for plants essential oil and its with organic acid it is compound to it is antibacterial, anti-oxidant, improve and give birth to
The research that the effects of long performance influences is less, how to develop more good natural green feed additive using related raw material and has become
For the important content and research hotspot of feed addictive development.
Meanwhile being found during essential oil and organic acid are applied to production as feed addictive, essential oil, which exists, easily to be waved
The problems such as hair aoxidizes, ingredient is indefinite and irritant；The problems such as organic acid cannot cross stomach there are the reaction time slowly, these problems
Have functions that cause essential oil and organic acid that can not play its more preferable.The coating type product of " essential oil+organic acid " just can be with
Solve their some limitations during feeding.Such coating type product has effects that in gastrointestinal tract slow release, can
It prevents essential oil and organic acid to be absorbed in alimentary canal leading portion, guarantees the more longlasting performance of the effect energy of essential oil and organic acid；Into
After enteron aisle, different starting dissociation programs of the organic acid carried according to different enteron aisle positions pH value, release hydrogen ions make enteron aisle
PH value decline inhibits most of harmful bacteria growth, while the hydrogen ion can also enter cell directly through cell membrane, reduce harmful
The activity and breeding of bacterium.At present on the market coating type " essential oil+organic acid " product that there are quality is inhomogenous, grain density is not high,
Chewing when coated layer is thin, agitating friction, shearing, occlusion, extruding and animals intolerant in feed processing, transporting procedures feed,
Easily keep coated layer damaged, the problems such as core material is exposed or even crushes, leading to be coated with effect reduces or failure.Therefore novel coating is developed
Type " essential oil+organic acid " product has become one of important content and research hotspot of feed addictive development.
Summary of the invention
In view of the deficiencies of the prior art, the object of the present invention is to provide a kind of derma type essential oil organic acid mixed feeds
Additive and its preparation method and application solves many drawbacks existing for current " essential oil+organic acid " product, achieves good
Effect.
To achieve the goals above, the technical scheme adopted by the invention is that:
A kind of derma type essential oil organic acid composite feed additive, the composite feed additive are graininess, from
It is interior to arrive outside respectively " plants essential oil+organic acid " core particles, " casing+organic acid " middle layer coatings and cross stomach outer layer coating layer；Institute
State " plants essential oil+organic acid " core particles component include Thymol, carvacrol, betel essential oil, Forsythia suspense essential oil, wormwood essential oil,
Cinnamon essential oil, citric acid, fumaric acid, sorbic acid, maltodextrin and hydroxypropyl methyl cellulose；The mistake " casing+organic acid "
The component of middle layer coatings includes sodium succinate, adipic acid sodium, fumaric acid sodium, ethyl cellulose, croscarmellose sodium
And dehydrated alcohol；The component for crossing stomach outer layer coating layer includes Eudragit Ⅲ, hydroxypropyl methyl cellulose and anhydrous
Ethyl alcohol.
The weight percent of each component is respectively as follows: Thymol 25%-30%, perfume (or spice) in " plants essential oil+organic acid " core particles
Celery phenol 15%-18%, betel essential oil 2%-3%, Forsythia suspense essential oil 3%-5%, wormwood essential oil 5%-8%, cinnamon essential oil 10%-
12%, citric acid 5%-8%, fumaric acid 1%-2%, sorbic acid 2%-5%, maltodextrin 12%-15% and hydroxypropyl methyl
Cellulose 15%-20%.
In " casing+organic acid " middle layer coatings the weight percent of each component be respectively as follows: sodium succinate 2%-10%, oneself
Diacid sodium 2%-10%, fumaric acid sodium 10%-20%, ethyl cellulose 3%-5%, croscarmellose sodium 25%-
30% and dehydrated alcohol 45%-55%.
The weight percent for crossing each component in stomach outer layer coating layer is respectively as follows: Eudragit Ⅲ 20%-40%, hydroxypropyl
Ylmethyl cellulose 15%-25% and dehydrated alcohol 45%-55%.
Betel essential oil the preparation method comprises the following steps: will dry betel stem and leaf by weight the ratio co-grinding of 1:2, crush
The ethanol solution of object and 70%-90% (v/v) are mixed in the ratio of solid-liquid ratio 1:5-10 (g/mL), in 200r/min stirring condition
Lower immersion 1h, 50 DEG C are heated to reflux 2h, collect filtrate, filter residue are repeated to extract 3 times, merging filtrate is concentrated with Rotary Evaporators
To the 1/3 of original volume, betel essential oil is obtained.
A kind of preparation method of composite feed additive, comprising the following steps:
(1) by each component of " plants essential oil+organic acid " core particles according to weight percent after mixing, be put into centrifugation system
The little particle of 40-60 mesh is made in grain machine；
(2) ethyl cellulose, croscarmellose sodium are dissolved in dehydrated alcohol, then be added sodium succinate,
Adipic acid sodium, fumaric acid sodium are uniformly mixed, and are configured to coating solution A；Little particle made from step (1) is put into fluidized bed coating pot
It is interior, coating solution A is sprayed into spray gun；Coating terminates for the first time after little particle weight gain 2%-5%；
(3) Eudragit Ⅲ, hydroxypropyl methyl cellulose are dissolved in dehydrated alcohol, are configured to coating solution B, it will
Particle is once coated with made from step (2) to be put into fluidized bed coating pot, sprays into coating solution B, weight gain 5%- with spray gun
Second of coating terminates after 10%；
(4) by the coating particle drying of step (3), vibrating screen is crossed, grain diameter controls in 10-30 mesh.
A kind of application of composite feed additive, composite feed additive additive amount in pannage is 0.1-0.15kg/
T, additive amount is 0.01-0.1kg/t in fowl feed.
The invention has the advantages that:
1, wormwood essential oil, betel essential oil, Forsythia suspense essential oil and the cinnamon essential oil that the present invention uses have complicated chemical component
With extensive pharmacological activity.(1) antibacterial.Wormwood essential oil main component is the terpenoids such as Cineole, camphor, borneol, limonene
Object；Betel essential oil main component be chavicol, chavicol, 4- allyl pyrocatechol, carvacrol, eugenol, p-cymene,
1, 8-Cineole, eugenol methyl ether, carypohyllene, cadinene；Forsythia suspense essential oil main component is alkene terpenoid and alkanes chemical combination
Object；Wherein sequiterpene and monoterpenes chemical component in wormwood essential oil, chavicol, eugenol etc. in betel essential oil, in cinnamon essential oil
Trans-cinnamic acid, trans-cinnamic aldehyde etc. all have bacteriostasis property.On the one hand its antifungal mechanism is Some essential oils ingredient such as thyme
The hydrophobic combination of phenol can directly act on the lipid bilayer on bacterial cell membrane, change the conformation of bacterial cell membrane
Become, cellular content is caused to leak.Derived essential oil can change Escherichia coli, staphylococcus aureus and salmonella simultaneously
The structure and ratio of fatty acid on cell membrane reduce the content of film unsaturated fatty acid, increase the content of saturated fatty acid, from
And the mobility of cell membrane is reduced, destroy the form of bacterial cell.Another aspect derived essential oil such as cinnamic acid acts not only on
Cell membrane, it can also penetrating cell film enter cytoplasm, by influencing the protein of the structural conservation in bacterial cell, prevent thin
Born of the same parents' division, to inhibit the activity of bacterium.(2) anti-inflammatory.Cineole, linalool, p-cymene etc. in the essential oils such as Forsythia suspense essential oil
Have the function of heat-clearing, anti-inflammatory.(3) body microbial balance is adjusted.The scholars such as Thompson A are research shows that cinnamon essential oil etc.
The quantity of bacterial species and the abundance of specific bacteria species can be changed, adjust intestinal flora balance.
Multiple components play pharmacological action jointly after scientific compatibility, are conducive to the antibacterial wide spectrum performance for enhancing compound, should
Compound additive can effectively inhibit staphylococcus aureus (antibacterial circle diameter > 15mm), Escherichia coli (antibacterial circle diameter >
22mm), Bacillus cercus (antibacterial circle diameter > 15.4mm), salmonella (antibacterial circle diameter > 14.7mm) etc..Other at
Divide the renewal rate that also can speed up enterocyte, stimulation enteron aisle villus is developed；Increase and digest and assimilate area, improves feeding
Amount；Antibacterial, anti-inflammatory promote cell metabolism.Compatibility is reasonable between each ingredient of essential oil, and effect is complementary, passes through biochemical reaction energy
Generate the synergistic functions such as preferable antibacterial, anti-oxidant, anti-inflammatory, raising animal immunizing power.
2, for citric acid, fumaric acid, sorbic acid that the present invention uses etc. for organic acid, antifungal mechanism is mainly that energy is competing
It strives, permeabilization bacterial outer membrane, changes osmotic pressure, macromolecular synthesis and induction peptide expression is inhibited to have in various antimicrobial mechanisms
Machine acid inhibits the effect of pathogen proliferation the most prominent in effect intracellular, and is effectively inhibiting the same of harmful bacteria in intestinal canal proliferation
When, the metabolism of the growth of probiotics such as Bacillus acidi lactici is not influenced.These three organic acids cooperate with compatibility, and acidity is strong, dosage is few, effect is fast,
Also changeable Intestinal Morphology, reduces chitterlings Crypt depth, increases height of naps while antibacterial.Citric acid can also be formed with copper
The high complex compound of biological value promotes the absorption and reservation of copper.
3, the present invention is the compound that a variety of natural plant essential oils and organic acid are combined through scientific matching, natural plants
Essential oil bacteriostasis under low ph condition caused by organic acid is stronger, is on the one hand H+The effect of itself, pH are reduced, bacterium institute
Place's environment changes, and reduces the activity of bacterium；On the other hand, pH reduces the phenol for causing institute's band on nonpolar phenolic compound
The degree of ionization of hydroxyl becomes smaller, and hydrophobicity increases, and phenols is more soluble in the lipid phase of bacterial cell membrane and the hydrophobic region of protein, and
Protein binding capacity on cell membrane is stronger, and bacteriostasis increases, therefore the compound additive is to conventional pathogenic bacteria such as large intestine
Bacillus has good restraining and sterilizing bacteria effect, and use that is alternative or reducing antibiotic has prevention to diarrhea caused by animal bacteria
And therapeutic effect, animal immunizing power can be enhanced, reduce respiratory disease incidence, while there is promotion animal digestion, improved
The effects of feed conversion rate.
4, external environment can be effectively relieved as added in Eudragit Ⅲ, hydroxypropyl methyl cellulose in stomach outer layer coating layer
Work temperature, external microbe, oxygen and internal gastric acid to the compound nature's mystery and caused by different degrees of destruction." casing+have
Organic acid contained in machine acid " middle layer coatings, can effectively buffer gastric acid and act on enteric materials structural damage, to keep away
Exempt from enteric materials reticular structure to collapse and lead to problems such as additive that can not reach enteron aisle, when composite feed additive enters enteron aisle
When, the organic acid in " casing+organic acid " middle layer coatings is replaced by gastric acid, with the intact coating material of reticular structure
Material is gradually dissolved by intestinal juice, and additive is uniformly discharged, and can effectively solve the efficient Utilizing question of essential oil+organic acid in this way.
The coating type additive as made from this method crosses stomach rate and is up to 92% or more, and small intestine release rate is up to 96% or more.
5, plants essential oil and organic acid are combined by the present invention using derma technology, and by rational proportion, are obtained
To a kind of novel coating type feed addictive, which can effectively pass through gastric acid, the slow release in animal intestinal tract, thus
Enable the effective component of product to reach animal intestinal tract, play physiology and immunization, while Biochemical Indices In Serum can be improved,
Immune Organs of Body index is improved, gastrointestinal content pH value is reduced, control harmful microbe growth keeps enteric microorganism
The balance of fauna improves small intestine Intestinal Morphology, increases intestine of young pigs mucous membrane permeability, protects gut barrier, improves nutrients
The utilization rate of matter improves production performance so that animal body be promoted to grow.
Specific embodiment
Specific embodiments of the present invention will be described in further detail with reference to embodiments.
Betel essential oil the preparation method comprises the following steps: will dry betel stem and leaf by weight the ratio co-grinding of 1:2, crush
The ethanol solution of object and 80% (v/v) are mixed in the ratio of solid-liquid ratio 1:10 (g/mL), are impregnated under 200r/min stirring condition
1h, 50 DEG C are heated to reflux 2h, collect filtrate, filter residue are repeated to extract 3 times, merging filtrate is concentrated into substance with Rotary Evaporators
Long-pending 1/3 obtains betel essential oil.
Embodiment 1
A kind of derma type essential oil organic acid composite feed additive (graininess) is respectively " plant essence from inside to outside
Oil+organic acid " core particles, " casing+organic acid " middle layer coatings and stomach outer layer coating layer excessively；
The weight percent of each component is respectively as follows: Thymol 25%, carvacrol in " plants essential oil+organic acid " core particles
15%, betel essential oil 2%, Forsythia suspense essential oil (CAS:68917-49-7) 3%, wormwood essential oil (CAS:8022-37-5) 5%, cortex cinnamomi
Essential oil (CAS:8007-80-5) 10%, citric acid 6%, fumaric acid 2%, sorbic acid 5%, maltodextrin 12% and hydroxypropyl first
Base cellulose 15%.
The weight percent of each component is respectively as follows: sodium succinate 5%, adipic acid in " casing+organic acid " middle layer coatings
Sodium 5%, fumaric acid sodium 10%, ethyl cellulose 5%, croscarmellose sodium 30% and dehydrated alcohol 45%.
The weight percent for crossing each component in stomach outer layer coating layer is respectively as follows: Eudragit Ⅲ 20%, hydroxypropyl first
Base cellulose 25% and dehydrated alcohol 55%.
Preparation method, comprising the following steps:
(1) by each component of " plants essential oil+organic acid " core particles according to weight percent after mixing, be put into centrifugation system
40-60 mesh little particle is made in grain machine；
(2) ethyl cellulose, croscarmellose sodium are dissolved in dehydrated alcohol, then be added sodium succinate,
Adipic acid sodium, fumaric acid sodium are uniformly mixed, and are configured to coating solution A；Little particle made from step (1) is put into fluidized bed coating pot
It is interior, coating solution A is sprayed into spray gun；Coating terminates for the first time after little particle weight gain 3%；
(3) Eudragit Ⅲ, hydroxypropyl methyl cellulose are dissolved in dehydrated alcohol, are configured to coating solution B, it will
Particle is once coated with made from step (2) to be put into fluidized bed coating pot, sprays into coating solution B with spray gun, the after weight gain 8%
Secondary coating terminates；
(4) by the coating particle drying of step (3), vibrating screen is crossed, product particle size controlling is in 10-30 mesh.
Embodiment 2
A kind of derma type essential oil organic acid composite feed additive (graininess) is respectively " plant essence from inside to outside
Oil+organic acid " core particles, " casing+organic acid " middle layer coatings and stomach outer layer coating layer excessively；
The weight percent of each component is respectively as follows: Thymol 25%, carvacrol in " plants essential oil+organic acid " core particles
16%, betel essential oil 3%, Forsythia suspense essential oil 3.5%, wormwood essential oil 5.5%, cinnamon essential oil 10%, citric acid 5%, fumaric acid
1%, sorbic acid 2%, maltodextrin 12% and hydroxypropyl methyl cellulose 17%.
The weight percent of each component is respectively as follows: sodium succinate 2%, adipic acid in " casing+organic acid " middle layer coatings
Sodium 2%, fumaric acid sodium 16%, ethyl cellulose 3%, croscarmellose sodium 25% and dehydrated alcohol 52%.
The weight percent for crossing each component in stomach outer layer coating layer is respectively as follows: Eudragit Ⅲ 30%, hydroxypropyl first
Base cellulose 15% and dehydrated alcohol 55%.
Preparation method and embodiment 1 are essentially identical, and the main distinction is, the weight gain of the present embodiment step (2) small particles
It is 5%, weight gain is 10% in step (3).
Embodiment 3
A kind of derma type essential oil organic acid composite feed additive (graininess) is respectively " plant essence from inside to outside
Oil+organic acid " core particles, " casing+organic acid " middle layer coatings and stomach outer layer coating layer excessively；
The weight percent of each component is respectively as follows: Thymol 30%, carvacrol in " plants essential oil+organic acid " core particles
15%, betel essential oil 2%, Forsythia suspense essential oil 3%, wormwood essential oil 5%, cinnamon essential oil 10%, citric acid 5%, fumaric acid 1%, mountain
Pears acid 2%, maltodextrin 12% and hydroxypropyl methyl cellulose 15%.
The weight percent of each component is respectively as follows: sodium succinate 10%, adipic acid in " casing+organic acid " middle layer coatings
Sodium 5%, fumaric acid sodium 10%, ethyl cellulose 4%, croscarmellose sodium 26%, dehydrated alcohol 45%.
The weight percent for crossing each component in stomach outer layer coating layer is respectively as follows: Eudragit Ⅲ 40%, hydroxypropyl first
Base cellulose 15%, dehydrated alcohol 45%.
Preparation method and embodiment 1 are essentially identical, and the main distinction is, the weight gain of the present embodiment step (2) small particles
It is 2%, weight gain is 5% in step (3).
Compliance test result
1, bacteriostatic experiment:
Fresh bacterial strain (staphylococcus aureus, Escherichia coli, Bacillus cercus, salmonella) slant culture is taken to use
It is 1 × 10 that sterile saline, which prepares concentration,4~1 × 105The bacteria suspension of CFU/ml, sterile 100 μ l of absorption bacteria suspension is in corresponding training
Primary surface is supported, coating is uniform.Additive of the present invention product 1g is set in ± 0.1 simulated intestinal fluid of 10mL pH=6.8, magnetic agitation
Accurately drawn after 4h 20 μ l drops in the sterile round scraps of paper (× 4mm) on, it is placed with after 37 DEG C of drying in media surface, it is negative
It compares the scraps of paper and 20 μ l sterile distilled waters is added dropwise.37 DEG C of cultures 18~for 24 hours, antibacterial circle diameter is measured, indicates antibacterial work greater than 7mm
With.
The result shows that additive of the present invention can effectively inhibit pathogenic entero becteria, such as staphylococcus aureus (antibacterial circle diameter
> 15mm), Escherichia coli (antibacterial circle diameter > 22mm), Bacillus cercus (antibacterial circle diameter > 15.4mm), salmonella
(antibacterial circle diameter > 14.7mm).
2, in-vitro simulated product digestion experiment:
(1) dissolution rate: according to the Pharmacopoeia of the People's Republic of China four 0931 dissolution rates of version in 2015 and drug release determination method
Second method (paddle method) weighs 9g additive product and is placed in 450mL pH=2 simulate the gastric juice, stirring slurry revolving speed 100r/min, surveys
The hydrochloric acid solution for determining process suitable concentration keeps pH=2.0 ± 0.1 of simulate the gastric juice, measuring the dissolution rate of different time.
(2) disintegration: the product after above-mentioned experiment out soluble in the stomach is cleaned with pure water to cleaning solution and is in neutrality, after taking cleaning
Product 1g is set in ± 0.1 simulated intestinal fluid of 25mL pH=6.8, magnetic agitation, and observation is not disintegrated the surplus of product particle, assessment
Disintegration.Experimental result is shown in Table 1.
The in-vitro simulated product digestion experiment of table 1
| Experimental project/time | 0.5h | 1h | 2h | 4h |
| Dissolution rate/% | 1.4 | 3.9 | 6.1 | 7.11 |
| Disintegration/% | 45 | 63 | 85 | 96.84 |
Experiment shows that coating type additive obtained crosses stomach rate by the method for the invention and is up to 92% or more, small enteric release
Rate is up to 96% or more.
3, weanling pig is tested:
This test uses 120 weanling pigs (28 ± 2d of Weaning Age, starting weight 7kg or so), is randomly divided at 3
Reason, 4 repetitions of each processing, 10 piglets of each repetition.Processing one is blank group, feeds basal diet, does not add feed and add
Add agent；It handles and adds novel fodder additive 0.1kg/t in embodiment 1 in two basal diets；It handles and is added in three basal diets
Novel fodder additive 0.1kg/t in embodiment 2.Experimental period is 30 days, and piglet uses free diet and drinking-water during test, is exempted from
Epidemic disease program and other feeding managements provide to carry out according to pig farm.
(1) influence of the novel fodder additive to weaned piglets
Average daily gain, daily ingestion amount, feedstuff-meat ratio and diarrhea rate during testing are calculated, the results are shown in Table 2.
Influence of 2 novel fodder additive of table to weaned piglets
| Project | Processing one | Processing two | Processing three |
| Starting weight (kg) | 7.08±0.75 | 6.99±0.69 | 7.02±1.02 |
| Daily gain (kg) | 0.45±0.37 | 0.55±0.29 | 0.53±0.34 |
| Daily ingestion amount (kg) | 0.63±0.15 | 0.72±0.11 | 0.73±0.14 |
| Feedstuff-meat ratio | 1.40±0.04 | 1.30±0.06 | 1.37±0.03 |
| Diarrhea rate (%) | 16.5±1.12 | 4.5±0.83 | 4.2±0.92 |
From Table 2, it can be seen that novel fodder additive of the present invention can effective prevention of diarrhea in piglets, compared to the blank group,
Grice diarrhoea rate significantly reduces；The novel fodder additive can also improve the daily gain of live pig simultaneously, and reduce feedstuff-meat ratio, explanation
Product of the present invention also has extraordinary help to promotion pig growth and raising efficiency of feed utilization.
(2) influence of the novel fodder additive to piglet respiratory disease
Observe each processing group pig whether there is or not cough, fever, nasal obstruction, sneeze, air-breathing be abnormal, the hair sound of snoring, dog sitting type abdominal respiration,
Or when food, eats several mouthfuls of new line mouths and deeply breathe or do the respiratory tracts abnormal symptoms such as ventilation movement, the results are shown in Table 3.
Influence of 3 novel fodder additive of table to piglet respiratory disease
| Project | Processing one | Processing two | Processing three |
| Respiratory diseases disease incidence (%) | 12.31±4.14 | 3.6±2.51 | 3.4±2.42 |
From table 3 it is observed that processing two and processing three piglets respiratory tract disease incidence be significantly lower than handle one (P <
0.05), illustrate that the humoral immunity and cellular immune function of body can be enhanced in novel fodder additive, improve natural killer cells
Quantity.
(3) blood parameters measure
At last 1 day of test, 5 pig acquisition blood samples are randomly selected in each repeating groups, is collected in and anti-coagulants is not added
In general blood collection tube, after standing 30min, 3000r/min is centrifuged 10min, isolates serum, and -20 DEG C of preservations after packing measure blood
Following index in clear: total serum protein (TP) is measured using biuret method, and seralbumin (ALB) uses bromocresol green
Method is measured, immunoglobulin A (Ig A), immunoglobulin G (Ig G), immunoglobulin M (Ig M) and Complement C_3 and
C4 is all made of pig ELISA kit and is measured, and the results are shown in Table 4.
Influence of 4 novel fodder additive of table to piglet blood biochemical indicator
| Index | Processing one | Processing two | Processing three |
| C3(mg/L) | 60.25±0.41 | 75.45±0.64 | 72.73±0.53 |
| C4(mg/L) | 6.34±0.04 | 9.53±0.06 | 9.82±0.03 |
| ALB(g/L) | 24.78±3.12 | 34.41±4.42 | 34.72±2.18 |
| TP(g/L) | 44.52±6.15 | 56.82±5.14 | 54.51±4.58 |
| IgA(g/L) | 0.064±0.03 | 0.073±0.03 | 0.071±0.02 |
| IgG(g/L) | 0.482±0.04 | 0.631±0.01 | 0.672±0.05 |
| IgM(g/L) | 0.182±0.06 | 0.185±0.05 | 0.189±0.02 |
As can be seen from Table 4, addition new additive agent be remarkably improved total serum protein in serum, seralbumin,
The content of immunoglobulin A, immunoglobulin G, immunoglobulin M and Complement C_3 and C4 illustrates to add organic acid and plant
Essential oil has humidification for the immune function of body.
(4) influence of the novel fodder additive to intestine of young pigs intestinal permeability
At last 1 day of test, 5 pig acquisition blood samples are randomly selected in each repeating groups, are collected in adopting for anticoagulant heparin
In blood vessel, 3000r/min is centrifuged 10min, separated plasma, and -70 DEG C of preservations after packing use spectrophotometry measurement blood plasma D- cream
Acid and diamine oxidase index, the results are shown in Table 5.
Influence of 5 novel fodder additive of table to intestine of young pigs intestinal permeability
| Project | Processing one | Processing two | Processing three |
| D-ALPHA-Hydroxypropionic acid (mg/L) | 5.83±0.38 | 3.82±0.61 | 3.73±0.28 |
| Diamine oxidase (U/mL) | 1.45±0.42 | 0.73±0.29 | 0.85±0.46 |
As can be seen from Table 5, compared with one group of processing, the D-ALPHA-Hydroxypropionic acid content pole in two, three groups of piglet blood plasma is handled
One group (P < 0.01) is substantially less than handled, reduces 34.48%, 36.02% respectively, the diamine oxidase activity in blood plasma is aobvious
It writes lower than one group (P < 0.05) of processing, reduces 49.66%, 41.38% respectively, illustrate that novel fodder additive increases piglet intestines
Mucous membrane permeability protects gut barrier.
(5) influence of the novel fodder additive to intestine of young pigs microbial flora
When testing the 30th day, collection fresh excreta, which is placed in ice chest, to be saved, and after transporting laboratory back, is weighed under aseptic condition
1.0g content is added sterile saline 9ml, rear doubling dilution fullys shake, is respectively coated Mai Kang in sterile test tube
Then the triumphant aerobic culture of agar medium carries out bacterium colony counting, the results are shown in Table 6 for 24 hours with MRS agar medium Anaerobic culturel 48h.
Influence of 6 novel fodder additive of table to intestine of young pigs flora
| Project | Processing one | Processing two | Processing three |
| Bacillus acidi lactici (cfu/g) | 7.15±0.05 | 7.85±0.08 | 7.97±0.06 |
| Escherichia coli (cfu/g) | 7.18±0.03 | 6.42±0.06 | 6.38±0.04 |
As can be seen from Table 6, processing two, three is compared with processing one, after feeding novel fodder additive in intestine of young pigs
The quantity of beneficial bacterium (Bacillus acidi lactici) significantly improves, and the quantity of harmful bacteria (Escherichia coli) is greatly reduced.
4, broiler chicken experimental verification:
1 age in days Huang plumage meat cock 180 of physical health is chosen, at random point 3 groups, each group of 5 repetitions, each repetition
12.Processing one is blank group, feeds basal diet, does not add feed addictive；Handle addition embodiment 1 in two basal diets
Middle novel fodder additive 0.06kg/t；It handles and adds novel fodder additive 0.06kg/t in embodiment 2 in three basal diets.
Test chicken daily hand fed 2 times is freely eaten, drinking-water.Immune, disinfection is carried out by broiler chicken conventional program.Experimental period 70 days.
When testing the 70th day, each broiler chicken for repeating selection 3 close to average weight takes a blood sample and butchers sampling.
(1) influence of the novel fodder additive to meat chicken growth performance
Every chicken birth weight is recorded when on-test, then at the 70th day 12 by only weighing, weigh preceding empty stomach 12h,
Calculate each stage average daily gain.As unit of repetition, precise simultaneously records each duplicate day inventory, clout amount and loss
Amount calculates each stage average daily gain, and then calculates each stage feed-weight ratio according to average daily gain and average daily gain.Together
When, dead chicken number is recorded daily, calculates each stage survival rate.
Influence of 7 novel fodder additive of table to meat chicken growth performance
| Project | Processing one | Processing two | Processing three |
| Average daily gain (g/ days) | 81.52±2.44 | 81.65±1.95 | 81.44±2.12 |
| Average daily gain (g/ days) | 31.45±0.54 | 32.75±0.71 | 32.41±0.93 |
| Feed-weight ratio | 2.59±0.07 | 2.49±0.04 | 2.51±0.06 |
| Survival rate (%) | 94.46±0.07 | 97.21±0.05 | 96.98±0.03 |
As can be seen from Table 7, novel fodder additive can increase average daily gain in same feed intake, drop
Low feed-weight ratio improves survival rate, significantly improves meat chicken growth performance.
(2) measurement of Immune Organs Index
Chicken is tested after the 70th day butchers, spleen, thymus gland and the bursa of farbricius is taken, sucks the blood on each organ with filter paper,
Removing fat is simultaneously weighed, and is calculated as follows:
Index and spleen index=(spleen weight/live-weight) × 100；
Thymus index=(chest gland weight/live-weight) × 100；
Bursal index=(bursa of farbricius weight/live-weight) × 100.
Influence of 8 novel fodder additive of table to broiler chicken Immune Organs Index
| Project | Processing one | Processing two | Processing three |
| Index and spleen index (g/kg) | 2.07±0.48 | 2.32±0.65 | 2.34±0.32 |
| Thymus index (g/kg) | 1.05±0.24 | 1.35±0.42 | 1.41±0.33 |
| Bursal index (g/kg) | 1.35±0.47 | 1.59±0.51 | 1.51±0.36 |
The raising of three kinds of spleen, thymus gland and the bursa of farbricius Immune Organs Indexes illustrates that novel fodder additive can pierce in table 8
Swash the immune response of broiler chicken, enhances immune function, promote the enhancing of broiler chicken entirety immunity function.
(3) measurement of Biochemical Indices In Serum
At test chicken the 70th day 12 by only weigh after, with vacuum blood collection tube carries out wing venous blood sampling 5ml, inclination standing,
After serum exudation, 3000r/min is centrifuged 15min, takes supernatant, is sub-packed in EP pipe, is placed in -80 DEG C and freezes.Using reagent
Box testing index Biochemical Indices In Serum, including total serum protein, albumin, uric acid content, are respectively adopted Coomassie Brilliant Blue, bromine
The green method of cresols and colorimetric method are measured.
Influence of 9 novel fodder additive of table to serum of broilers biochemical indicator
| Project | Processing one | Processing two | Processing three |
| Albumin (g/L) | 29.17±1.28 | 32.52±1.35 | 32.34±1.77 |
| Total protein (g/L) | 30.25±4.22 | 35.15±5.41 | 34.67±4.73 |
| Uric acid (μm ol/L) | 301.11±20.07 | 261.35±28.62 | 274.44±26.56 |
As can be seen from Table 9, novel fodder additive can significantly improve serum of broilers biochemical indicator, improve albumin and
Total protein levels reduce uric acid content, improve body to the utilization rate of protein, promote protein in the intracorporal deposition of machine, from
And promote the growth of broiler chicken.
Claims (7)
1. a kind of derma type essential oil organic acid composite feed additive, which is characterized in that the composite feed additive is
Graininess is respectively " plants essential oil+organic acid " core particles, " casing+organic acid " middle layer coatings from inside to outside and crosses stomach outer layer
Coatings；The component of " plants essential oil+organic acid " core particles include Thymol, carvacrol, betel essential oil, Forsythia suspense essential oil,
Wormwood essential oil, cinnamon essential oil, citric acid, fumaric acid, sorbic acid, maltodextrin and hydroxypropyl methyl cellulose；Mistake " the casing
The component of+organic acid " middle layer coatings includes sodium succinate, adipic acid sodium, fumaric acid sodium, ethyl cellulose, cross-linked carboxymethyl
Sodium cellulosate and dehydrated alcohol；The component for crossing stomach outer layer coating layer includes Eudragit Ⅲ, hydroxypropyl methyl fiber
Element and dehydrated alcohol.
2. composite feed additive according to claim 1, which is characterized in that " plants essential oil+organic acid " core particles
The weight percent of middle each component be respectively as follows: Thymol 25%-30%, carvacrol 15%-18%, betel essential oil 2%-3%,
Forsythia suspense essential oil 3%-5%, wormwood essential oil 5%-8%, cinnamon essential oil 10%-12%, citric acid 5%-8%, fumaric acid 1%-
2%, sorbic acid 2%-5%, maltodextrin 12%-15% and hydroxypropyl methyl cellulose 15%-20%.
3. composite feed additive according to claim 1, which is characterized in that " casing+organic acid " the middle layer coating
The weight percent of each component is respectively as follows: sodium succinate 2%-10%, adipic acid sodium 2%-10%, fumaric acid sodium 10%- in layer
20%, ethyl cellulose 3%-5%, croscarmellose sodium 25%-30% and dehydrated alcohol 45%-55%.
4. composite feed additive according to claim 1, which is characterized in that described to cross each component in stomach outer layer coating layer
Weight percent be respectively as follows: Eudragit Ⅲ 20%-40%, hydroxypropyl methyl cellulose 15%-25% and anhydrous second
Alcohol 45%-55%.
5. according to right want 1 described in composite feed additive, which is characterized in that the betel essential oil the preparation method comprises the following steps: will
The stem and leaf of dry betel by weight the ratio co-grinding of 1:2, press by the ethanol solution of crushed material and 70%-90% (v/v)
The ratio of solid-liquid ratio 1:5-10 (g/mL) mixes, and 1h is impregnated under 200r/min stirring condition, and 50 DEG C are heated to reflux 2h, collects filter
Filter residue is repeated to extract 3 times by liquid, and merging filtrate is concentrated into the 1/3 of original volume with Rotary Evaporators, obtains betel essential oil.
6. a kind of preparation method of the composite feed additive as described in claim any one of 1-5, which is characterized in that including following
Step:
(1) by each component of " plants essential oil+organic acid " core particles according to weight percent after mixing, be put into centrifugal granulator
The little particle of 40-60 mesh is made；
(2) ethyl cellulose, croscarmellose sodium are dissolved in dehydrated alcohol, then be added sodium succinate, oneself two
Sour sodium, fumaric acid sodium are uniformly mixed, and are configured to coating solution A；Little particle made from step (1) is put into fluidized bed coating pot,
Coating solution A is sprayed into spray gun；Coating terminates for the first time after little particle weight gain 2%-5%；
(3) Eudragit Ⅲ, hydroxypropyl methyl cellulose are dissolved in dehydrated alcohol, are configured to coating solution B, by step
(2) it is once coated with particle made to be put into fluidized bed coating pot, after spray gun penetrating coating solution B, weight gain 5%-10%
Second of coating terminates；
(4) by the coating particle drying of step (3), vibrating screen is crossed, grain diameter controls in 10-30 mesh.
7. a kind of application of composite feed additive as described in any one in claim 1-5, which is characterized in that mixed feed adds
Adding agent additive amount in pannage is 0.1-0.15kg/t, and additive amount is 0.01-0.1kg/t in fowl feed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910462347.0A CN110100965A (en) | 2019-05-30 | 2019-05-30 | A kind of derma type essential oil organic acid composite feed additive and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910462347.0A CN110100965A (en) | 2019-05-30 | 2019-05-30 | A kind of derma type essential oil organic acid composite feed additive and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110100965A true CN110100965A (en) | 2019-08-09 |
Family
ID=67493050
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910462347.0A Pending CN110100965A (en) | 2019-05-30 | 2019-05-30 | A kind of derma type essential oil organic acid composite feed additive and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110100965A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112042830A (en) * | 2020-09-27 | 2020-12-08 | 中国农业大学 | Broiler feed additive and application thereof |
| CN113331306A (en) * | 2021-06-03 | 2021-09-03 | 北京中科万联科技有限公司 | Additive for improving livestock and poultry physiological function based on yucca, and preparation method and application thereof |
| CN113662097A (en) * | 2021-08-24 | 2021-11-19 | 广东酸动力生物科技有限公司 | Formic acid type intestinal tract slow-release acidifier and preparation method thereof |
| CN113679608A (en) * | 2021-08-24 | 2021-11-23 | 广东酸动力生物科技有限公司 | Preparation facilities of formic acid type intestinal slowly-releasing type acidifier |
| CN114557407A (en) * | 2022-03-23 | 2022-05-31 | 上海美农生物科技股份有限公司 | Plant essential oil feed additive for pigs and preparation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1518417A (en) * | 2001-06-21 | 2004-08-04 | 协和发酵工业株式会社 | Process for producing plant extract containing plant powder |
| CN102106461A (en) * | 2010-12-28 | 2011-06-29 | 安徽泰格生物技术股份有限公司 | Enveloping acidifier for feed and preparation method thereof |
| US20110230560A1 (en) * | 2007-08-03 | 2011-09-22 | Vetagro S.P.A. | Synergetic composition comprising flavouring substances and organic acids and use thereof |
| CN103549162A (en) * | 2013-11-02 | 2014-02-05 | 成都雪樱动物科技实业有限公司 | Novel feed additive and preparation method thereof |
| CN103583922A (en) * | 2013-11-25 | 2014-02-19 | 广州美瑞泰科生物工程技术有限公司 | Poultry used disease-resistant growth promotion green feed additive and preparing method thereof |
| CN105076717A (en) * | 2015-07-14 | 2015-11-25 | 上海美农生物科技股份有限公司 | Formula and processing method of feed additive for improving production performance of animals and guaranteeing health of alimentary canals |
| CN108740371A (en) * | 2018-04-04 | 2018-11-06 | 广州智特奇生物科技股份有限公司 | Enteric capsulating material, using coated zinc oxide of the capsulating material and preparation method thereof |
-
2019
- 2019-05-30 CN CN201910462347.0A patent/CN110100965A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1518417A (en) * | 2001-06-21 | 2004-08-04 | 协和发酵工业株式会社 | Process for producing plant extract containing plant powder |
| US20110230560A1 (en) * | 2007-08-03 | 2011-09-22 | Vetagro S.P.A. | Synergetic composition comprising flavouring substances and organic acids and use thereof |
| CN102106461A (en) * | 2010-12-28 | 2011-06-29 | 安徽泰格生物技术股份有限公司 | Enveloping acidifier for feed and preparation method thereof |
| CN103549162A (en) * | 2013-11-02 | 2014-02-05 | 成都雪樱动物科技实业有限公司 | Novel feed additive and preparation method thereof |
| CN103583922A (en) * | 2013-11-25 | 2014-02-19 | 广州美瑞泰科生物工程技术有限公司 | Poultry used disease-resistant growth promotion green feed additive and preparing method thereof |
| CN105076717A (en) * | 2015-07-14 | 2015-11-25 | 上海美农生物科技股份有限公司 | Formula and processing method of feed additive for improving production performance of animals and guaranteeing health of alimentary canals |
| CN108740371A (en) * | 2018-04-04 | 2018-11-06 | 广州智特奇生物科技股份有限公司 | Enteric capsulating material, using coated zinc oxide of the capsulating material and preparation method thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112042830A (en) * | 2020-09-27 | 2020-12-08 | 中国农业大学 | Broiler feed additive and application thereof |
| CN113331306A (en) * | 2021-06-03 | 2021-09-03 | 北京中科万联科技有限公司 | Additive for improving livestock and poultry physiological function based on yucca, and preparation method and application thereof |
| CN113662097A (en) * | 2021-08-24 | 2021-11-19 | 广东酸动力生物科技有限公司 | Formic acid type intestinal tract slow-release acidifier and preparation method thereof |
| CN113679608A (en) * | 2021-08-24 | 2021-11-23 | 广东酸动力生物科技有限公司 | Preparation facilities of formic acid type intestinal slowly-releasing type acidifier |
| CN113679608B (en) * | 2021-08-24 | 2024-03-19 | 广东酸动力生物科技有限公司 | Preparation facilities of formic acid type intestinal slowly-releasing acidulant |
| CN114557407A (en) * | 2022-03-23 | 2022-05-31 | 上海美农生物科技股份有限公司 | Plant essential oil feed additive for pigs and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110100965A (en) | A kind of derma type essential oil organic acid composite feed additive and its preparation method and application | |
| CN110100956A (en) | It is a kind of with anti-oxidant and immunoloregulation function " acid+essential oil " coating type feed addictive and its preparation method and application | |
| US20200368301A1 (en) | Novel Composition to Improve Gut Health and Animal Performance and Methods of Making the Same | |
| CN103355545B (en) | Fermentation type botanical weaned piglet feed additive | |
| CN114007442A (en) | Feed additive for preventing African swine fever and other similar swine diseases or replacing antibiotic growth promoter, and preparation method and application thereof | |
| CN104719671A (en) | Compound essential oil for preventing diarrhea of piglets as well as preparation method and application of compound essential oil | |
| CN113812528A (en) | Plant extract additive for improving intestinal health of livestock and poultry | |
| CN105287790B (en) | A kind of fevervine extract and its application | |
| CN103719623A (en) | Traditional Chinese medicine feed additive capable of preventing or relieving stress syndrome of weaned pigs and preparation method of feed additive | |
| CN101912042A (en) | Pure natural plant feed additive for preventing and controlling diarrhea and application | |
| CN110314178B (en) | Pure natural compound essential oil with broad-spectrum bactericidal activity and preparation and application thereof | |
| CN110679737A (en) | Liver-protecting kidney-protecting mould-removing feed additive for livestock and poultry as well as preparation method and application thereof | |
| JP2018509161A (en) | Archaea in bioactive animal feed, method for producing the composition, and method using the composition | |
| CN114568602A (en) | Traditional Chinese medicine compound for improving growth performance and organism immunity of broiler chickens and preparation method and application thereof | |
| Alagbe | Clerodendron splendens leaf extract supplementation in weaner rabbits: impact on growth performance, haematology and intestinal microbial population | |
| CN107183310A (en) | A kind of feed addictive of effective enhancing pigling immunity | |
| CN112807348B (en) | Disease-resistant and growth-promoting traditional Chinese medicine compound, and preparation and application thereof | |
| CN111165651A (en) | Additive premixed feed and application thereof | |
| CN110604757A (en) | Compound preparation for strengthening body resistance and detoxifying, and preparation method and application thereof | |
| Chekav et al. | Effects of dietary Persian hogweed (Heracleum persicum) powder on growth performance, hematological and immune indices, and resistance against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss) | |
| Devi et al. | Dietary Addition of Black Cumin (Nigella Sativa) Seed on the Performance of Broiler Chicken during Summer Season | |
| Thuy et al. | Effect of adding guava leaf (Psidium guajava) and garlic (Allium sativum) powders in diets on growth performance and diarrhea incidence of weaned piglets | |
| CN105558350A (en) | Feed containing Chinese herbal medicine compound crude polysaccharide and preparation method of feed | |
| Elnaggar et al. | Impact Of Ginseng (Panax Ginseng) On Growth Performance, Blood Biochemical Parameters And Antioxidative Status Of Japanese Quail | |
| CN109620877A (en) | Pure Chinese medicine fowl antibacterium disease preparation and preparation method thereof and application method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |