CN108651710A - A kind of Efficient antibacterial composite essential oil and preparation method thereof - Google Patents
A kind of Efficient antibacterial composite essential oil and preparation method thereof Download PDFInfo
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- CN108651710A CN108651710A CN201810682814.6A CN201810682814A CN108651710A CN 108651710 A CN108651710 A CN 108651710A CN 201810682814 A CN201810682814 A CN 201810682814A CN 108651710 A CN108651710 A CN 108651710A
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- 239000000341 volatile oil Substances 0.000 title claims abstract description 89
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 62
- 239000002131 composite material Substances 0.000 title claims abstract description 57
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- MGSRCZKZVOBKFT-UHFFFAOYSA-N thymol Chemical compound CC(C)C1=CC=C(C)C=C1O MGSRCZKZVOBKFT-UHFFFAOYSA-N 0.000 claims abstract description 58
- GHVNFZFCNZKVNT-UHFFFAOYSA-N decanoic acid Chemical compound CCCCCCCCCC(O)=O GHVNFZFCNZKVNT-UHFFFAOYSA-N 0.000 claims abstract description 56
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 56
- RRAFCDWBNXTKKO-UHFFFAOYSA-N eugenol Chemical compound COC1=CC(CC=C)=CC=C1O RRAFCDWBNXTKKO-UHFFFAOYSA-N 0.000 claims abstract description 54
- RECUKUPTGUEGMW-UHFFFAOYSA-N carvacrol Chemical compound CC(C)C1=CC=C(C)C(O)=C1 RECUKUPTGUEGMW-UHFFFAOYSA-N 0.000 claims abstract description 30
- HHTWOMMSBMNRKP-UHFFFAOYSA-N carvacrol Natural products CC(=C)C1=CC=C(C)C(O)=C1 HHTWOMMSBMNRKP-UHFFFAOYSA-N 0.000 claims abstract description 30
- 235000007746 carvacrol Nutrition 0.000 claims abstract description 30
- WYXXLXHHWYNKJF-UHFFFAOYSA-N isocarvacrol Natural products CC(C)C1=CC=C(O)C(C)=C1 WYXXLXHHWYNKJF-UHFFFAOYSA-N 0.000 claims abstract description 30
- 239000005844 Thymol Substances 0.000 claims abstract description 29
- 229960000790 thymol Drugs 0.000 claims abstract description 29
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- 108010070257 neotame Proteins 0.000 claims abstract description 28
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- 239000005770 Eugenol Substances 0.000 claims abstract description 27
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- WBYWAXJHAXSJNI-SREVYHEPSA-N Cinnamic acid Chemical compound OC(=O)\C=C/C1=CC=CC=C1 WBYWAXJHAXSJNI-SREVYHEPSA-N 0.000 claims abstract description 25
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- WBYWAXJHAXSJNI-UHFFFAOYSA-N methyl p-hydroxycinnamate Natural products OC(=O)C=CC1=CC=CC=C1 WBYWAXJHAXSJNI-UHFFFAOYSA-N 0.000 claims abstract description 25
- BGNXCDMCOKJUMV-UHFFFAOYSA-N Tert-Butylhydroquinone Chemical compound CC(C)(C)C1=CC(O)=CC=C1O BGNXCDMCOKJUMV-UHFFFAOYSA-N 0.000 claims abstract description 19
- 235000019482 Palm oil Nutrition 0.000 claims abstract description 18
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- 235000019281 tert-butylhydroquinone Nutrition 0.000 claims abstract description 18
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- 239000000243 solution Substances 0.000 description 29
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 20
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- 206010059866 Drug resistance Diseases 0.000 description 2
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- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- KJPRLNWUNMBNBZ-QPJJXVBHSA-N (E)-cinnamaldehyde Chemical compound O=C\C=C\C1=CC=CC=C1 KJPRLNWUNMBNBZ-QPJJXVBHSA-N 0.000 description 1
- 241000607528 Aeromonas hydrophila Species 0.000 description 1
- 206010004016 Bacterial diarrhoea Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 208000022555 Genital disease Diseases 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
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- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
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- AXMVYSVVTMKQSL-UHFFFAOYSA-N UNPD142122 Natural products OC1=CC=C(C=CC=O)C=C1O AXMVYSVVTMKQSL-UHFFFAOYSA-N 0.000 description 1
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- 235000004279 alanine Nutrition 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
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- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical compound OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
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- 239000003153 chemical reaction reagent Substances 0.000 description 1
- KJPRLNWUNMBNBZ-UHFFFAOYSA-N cinnamic aldehyde Natural products O=CC=CC1=CC=CC=C1 KJPRLNWUNMBNBZ-UHFFFAOYSA-N 0.000 description 1
- 229940117916 cinnamic aldehyde Drugs 0.000 description 1
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- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
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- 229910021645 metal ion Inorganic materials 0.000 description 1
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- 239000011780 sodium chloride Substances 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- GOLXNESZZPUPJE-UHFFFAOYSA-N spiromesifen Chemical compound CC1=CC(C)=CC(C)=C1C(C(O1)=O)=C(OC(=O)CC(C)(C)C)C11CCCC1 GOLXNESZZPUPJE-UHFFFAOYSA-N 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- 150000003505 terpenes Chemical class 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of Efficient antibacterial composite essential oils and preparation method thereof.The Efficient antibacterial composite essential oil includes following components：Carvacrol, Thymol, cinnamic acid, eugenol, fennel oil, perilla herb oil, pungent capric acid, lactic acid, tert-butylhydroquinone, neotame solution, white carbon, palm oil.The Efficient antibacterial composite essential oil of the present invention has good antibacterial ability, and being confirmed by bacteriostatic test has stronger fungistatic effect under the proportioning of plurality of raw materials；The Efficient antibacterial composite essential oil of the present invention passes through rational proportion, preparation process is improved simultaneously, increases pungent capric acid and lactic acid as synergist and chelating agent, the antibacterial ability of composite essential oil is made to greatly improve, and there is preferable antioxygenic property, effectively improve growth of animal performance；Processing technology of the present invention is simple, of low cost, green safe, and the antibacterial composite essential oil is enable preferably to be promoted and applied as feeding antibiotic substitute.
Description
Technical field
The present invention relates to feed additive field, specifically a kind of Efficient antibacterial composite essential oil and preparation method thereof.
Background technology
For to animal health and the considerations of improve growth performance, used in feed and breeding process in animal husbandry system
Various antibiotic cause abuse of antibiotics and bacterium to generate drug resistance, and food quality declines, and environmental pollution is serious, people's health
Unprecedentedly threatened.So people have active demand to the food of health and good environment.American-European and other many countries are
Through forbidding or will forbid adding antibiotic in feed successively, reduce various antibiotic farm use scope.In feed
With prohibit in farm it is anti-and subtract it is anti-be world's animal husbandry inexorable trend, Chinese taboo is anti-to be subtracted anti-measure and is also pushing away by easy stages
Row.
Essential oil resists the during of subtracting anti-to play the part of emphatically because essence has the effects that bactericidal, growth promotion, raising are immune prohibiting
Want role.The main function of plants essential oil has：（1）Antibacterial action；Essential oil is to Escherichia coli, salmonella, Staphylococcus aureus
The pathogenic bacteria such as vibrios, Aeromonas hydrophila, streptococcus in bacterium, aquatic livestock have very strong inhibition or killing effect, to animal
Intestinal bacterial diarrhea, genital disease and breathing problem have prevention and mitigation well, while to mould and posting
It is infested to have compared with high inhibition effect.（2）Growth promoting function；It safeguards that intestinal flora balances, activates gastrointestinal smooth muscle, promote digestion
The secretion of liquid and the activity for improving digestive ferment improve absorption of the animal to nutriment, improve feedstuff-meat ratio, play somatotrophic work
With.（3）Improve animal body immunity；In range of doses, essential oil can promote the growth and development of Immune Organs of Body, bright
The aobvious weight for increasing fowl poultry immune organ, improves the phagocytic activity of macrophage in livestock and poultry body, enhances body anti-inflammatory effect, carries
Antibody level in high body.（4）Antioxidation；Derived essential oil contains phenolic hydroxyl group, and mostly terpenoid, has very strong
Antioxidation.It generates more stable benzene oxygen radical by dehydrogenation reaction and terminates chain reaction, can also make cell membrane
It is not oxidized with the unsaturated fatty acid in mitochondria, and then the structure of protective film and maintenance cell function.Body can be activated
The sources Inner property antioxidant, and adjustable anti-oxidative defense enzyme activity.（5）Improve meat flavor and be waterpower；As fragrance
Type essential oil, to glutamic acid related with delicate flavour and sweet taste, glycine, threonine, serine and alanine etc. in animal muscle
The content of amino acid has different degrees of raising, improves the content of a variety of unsaturated fatty acids in muscle, increases meat flavor
Be waterpower.（6）Reduce exhaust gas discharge；Essential oil reduces the discharge of poultry ammonia and hydrogen sulfide, purifies colony house air, improves cultivation
Environment.（7）Promote appetite；There is special essential oil fragrance, stimulate the appetite of animal, increases feed intake.Glossopharyngeal nerve can be stimulated
And olfactory organ, promote the secretion of saliva, gastric juice and enteron aisle digestive ferment, the digestibility for the substance that has additional nutrients.（8）Synergistic effect；
There are synergistic effect, enhancings when being used in conjunction with the enzyme preparation in feed addictive, organic acid, amino-acid chelate, antibiotic
Its function and effect.From the foregoing, it will be observed that it includes the multiple functions such as antibacterial, growth promotion that essential oil, which has, as a kind of green, it is efficient, do not produce
Raw drug resistance and the residual novel fodder additive of medicine have broad application prospects.But existing essential oil type feed addictive is still
Have the shortcomings that antibacterial ability is poor.Therefore, a kind of Efficient antibacterial composite essential oil of present invention offer and preparation method thereof.
Invention content
The present invention starts with from the compound proportion of various monomer essential oils in order to improve the antibacterial ability of essential oil, passes through antibacterial examination
Selection optimum compound proportion example is tested, addition synergist improves sterilizing ability, improves its palatability by adjusting mouthfeel and smell, finally
Essential oil special packet is covered smell, is improved the anti-oxidant and anti-developability of essential oil by process.Product produced by the invention is
A kind of antibacterial growth promotion ability is strong, resistance is strong, green high-efficient novel fodder additive.
To achieve the above object, the present invention provides the following technical solutions：
A kind of Efficient antibacterial composite essential oil, the Efficient antibacterial composite essential oil includes following components：Carvacrol, Thymol, meat
Cinnamic aldehyde, eugenol, fennel oil, perilla herb oil, pungent capric acid, lactic acid, tert-butylhydroquinone, neotame solution, white carbon, palm oil.
As a further solution of the present invention：The weight ratio of pungent capric acid and lactic acid is 3:1, the total weight of pungent capric acid and lactic acid
Account for the 4% of Efficient antibacterial composite essential oil.
As a further solution of the present invention：The weight ratio of carvacrol and Thymol is 3:2；Eugenol and cinnamic acid
Weight ratio is 1:9.
As a further solution of the present invention：The total weight of the total weight and eugenol, cinnamic acid of carvacrol, Thymol
The ratio between be 1:1.
As a further solution of the present invention：The weight ratio of fennel oil and perilla herb oil is 1:1, fennel oil and perilla herb oil it is total
Weight accounts for the 4% of Efficient antibacterial composite essential oil.
As a further solution of the present invention：The neotame solution is using neotame with absolute ethyl alcohol according to 1：50 weight ratio
It mixes.
As a further solution of the present invention：The Efficient antibacterial composite essential oil includes following components：Carvacrol 1.5-6
It is part, 1-4 parts of Thymol, 2.25-9 parts of cinnamic acid, 0.25-1 parts of eugenol, 0.5-2 parts of fennel oil, 0.5-2 parts of perilla herb oil, pungent
0.75-3 parts of capric acid, 0.25-1 parts of lactic acid, 0.01-0.04 parts of tert-butylhydroquinone, 0.35-1.4 parts of neotame solution, white carbon
5.49-21.96 37-148 parts of part, palm oil.
As a further solution of the present invention：The Efficient antibacterial composite essential oil includes following components：3 parts of carvacrol, hundred
In fragrant 2 parts of phenol, 4.5 parts of cinnamic acid, 0.5 part of eugenol, 1 part of fennel oil, 1 part of perilla herb oil, 1.5 parts of pungent capric acid, 0.5 part of lactic acid,
0.02 part of tert-butylhydroquinone, 0.7 part of neotame solution, 10.98 parts of white carbon, 74 parts of palm oil.
A kind of preparation method of Efficient antibacterial composite essential oil, includes the following steps：（1）By carvacrol, Thymol, Chinese cassia tree
Aldehyde, eugenol, fennel oil, perilla herb oil are added in reaction kettle and are stirred, fully spare after dissolving mixing；（2）It then will be pungent
Capric acid, lactic acid, tert-butylhydroquinone, neotame solution are added sequentially to be stirred in reaction kettle；White carbon is thrown after mixing
Enter and adsorbs mixing rapidly into reaction kettle；（3）First palm oil is melted in advance, is then then added in reaction kettle and is sufficiently mixed
It is even；Use spraying apparatus later, the above mixed liquor be sprayed in closed cooling device, form cooling fine particle, sieving to get
Finished product.
As a further solution of the present invention：Include the following steps：（1）By carvacrol, Thymol, cinnamic acid, cloves
Phenol, fennel oil, perilla herb oil are added in reaction kettle and are stirred, and temperature of reaction kettle is 50 DEG C, speed of agitator 40rpm/min,
It is fully spare after dissolving mixing；（2）Then pungent capric acid, lactic acid, tert-butylhydroquinone, neotame solution are added sequentially to react
In kettle, 5min is stirred under the rotating speed of 40rpm/min；White carbon is put into adsorb rapidly in reaction kettle after mixing and is mixed
It is even；（3）First palm oil is melted at a temperature of 70 DEG C in advance, is then then added in reaction kettle, is sufficiently mixed at 50 DEG C uniformly；
Use spraying apparatus later, the above mixed liquor be sprayed in closed cooling device, form cooling fine particle, sieving to get at
Product.
Compared with prior art, the beneficial effects of the invention are as follows：
The Efficient antibacterial composite essential oil of the present invention has good antibacterial ability, and matching for plurality of raw materials is confirmed by bacteriostatic test
There is stronger fungistatic effect than lower：Such as the best compounding weight ratio of carvacrol and Thymol is 3:2, cinnamic acid and cloves
The best compounding weight ratio of phenol is 9:1；Carvacrol, the total weight of Thymol and the ratio between the total weight of eugenol, cinnamic acid are
1:1；The Efficient antibacterial composite essential oil of the present invention passes through rational proportion, while improving preparation process, increases pungent capric acid and lactic acid is made
For synergist and chelating agent, the antibacterial ability of composite essential oil is made to greatly improve, and there is preferable antioxygenic property, effectively improved
Growth of animal performance；Processing technology of the present invention is simple, of low cost, green safe, makes the antibacterial composite essential oil as feeding anti-
Raw element substitute can be promoted and applied preferably.
Specific implementation mode
The technical solution of this patent is described in more detail With reference to embodiment.
Embodiment 1
A kind of Efficient antibacterial composite essential oil, the Efficient antibacterial composite essential oil includes following components：1.5 parts of carvacrol, thyme
1 part of phenol, 2.25 parts of cinnamic acid, 0.25 part of eugenol, 0.5 part of fennel oil, 0.5 part of perilla herb oil, 0.75 part of pungent capric acid, lactic acid 0.25
Part, 0.01 part of tert-butylhydroquinone, 0.35 part of neotame solution, 5.49 parts of white carbon, 37 parts of palm oil.The neotame solution is adopted
With neotame and absolute ethyl alcohol according to 1：50 weight ratio mixes.
A kind of preparation method of Efficient antibacterial composite essential oil, includes the following steps：（1）By carvacrol, Thymol, Chinese cassia tree
Aldehyde, eugenol, fennel oil, perilla herb oil are added in reaction kettle and are stirred, and temperature of reaction kettle is 40 DEG C, and speed of agitator is
30rpm/min, it is fully spare after dissolving mixing；（2）Then successively by pungent capric acid, lactic acid, tert-butylhydroquinone, neotame solution
It is added in reaction kettle, 3min is stirred under the rotating speed of 30rpm/min；White carbon put into after mixing fast in reaction kettle
Speed absorption mixing；（3）First palm oil is melted at a temperature of 60 DEG C in advance, is then then added in reaction kettle, at 40 DEG C fully
It is uniformly mixed；Spraying apparatus is used later, the above mixed liquor is sprayed in closed cooling device, forms cooling fine particle, mistake
Sieve is to get finished product.
Embodiment 2
A kind of Efficient antibacterial composite essential oil, the Efficient antibacterial composite essential oil includes following components：6 parts of carvacrol, Thymol 4
Part, 9 parts of cinnamic acid, 1 part of eugenol, 2 parts of fennel oil, 2 parts of perilla herb oil, 3 parts of pungent capric acid, 1 part of lactic acid, tert-butylhydroquinone
0.04 part, 1.4 parts of neotame solution, 21.96 parts of white carbon, 148 parts of palm oil.The neotame solution uses neotame and absolute ethyl alcohol
According to 1：50 weight ratio mixes.
A kind of preparation method of Efficient antibacterial composite essential oil, includes the following steps：（1）By carvacrol, Thymol, Chinese cassia tree
Aldehyde, eugenol, fennel oil, perilla herb oil are added in reaction kettle and are stirred, and temperature of reaction kettle is 55 DEG C, and speed of agitator is
50rpm/min, it is fully spare after dissolving mixing；（2）Then successively by pungent capric acid, lactic acid, tert-butylhydroquinone, neotame solution
It is added in reaction kettle, 8min is stirred under the rotating speed of 40rpm/min；White carbon put into after mixing fast in reaction kettle
Speed absorption mixing；（3）First palm oil is melted at a temperature of 75 DEG C in advance, is then then added in reaction kettle, at 55 DEG C fully
It is uniformly mixed；Spraying apparatus is used later, the above mixed liquor is sprayed in closed cooling device, forms cooling fine particle, mistake
Sieve is to get finished product.
Embodiment 3
A kind of Efficient antibacterial composite essential oil, the Efficient antibacterial composite essential oil includes following components：3 parts of carvacrol, Thymol 2
Part, 4.5 parts of cinnamic acid, 0.5 part of eugenol, 1 part of fennel oil, 1 part of perilla herb oil, 1.5 parts of pungent capric acid, 0.5 part of lactic acid, tertiary butyl pair
0.02 part of benzenediol, 0.7 part of neotame solution, 10.98 parts of white carbon, 74 parts of palm oil.The neotame solution uses neotame and nothing
Water-ethanol is according to 1：50 weight ratio mixes.
A kind of preparation method of Efficient antibacterial composite essential oil, includes the following steps：（1）By carvacrol, Thymol, Chinese cassia tree
Aldehyde, eugenol, fennel oil, perilla herb oil are added in reaction kettle and are stirred, and temperature of reaction kettle is 50 DEG C, and speed of agitator is
40rpm/min, it is fully spare after dissolving mixing；（2）Then successively by pungent capric acid, lactic acid, tert-butylhydroquinone, neotame solution
It is added in reaction kettle, 5min is stirred under the rotating speed of 40rpm/min；White carbon put into after mixing fast in reaction kettle
Speed absorption mixing；（3）First palm oil is melted at a temperature of 70 DEG C in advance, is then then added in reaction kettle, at 50 DEG C fully
It is uniformly mixed；Spraying apparatus is used later, the above mixed liquor is sprayed in closed cooling device, forms cooling fine particle, mistake
Sieve is to get finished product.
Experimental example
By carvacrol and Thymol respectively with 1:2,2:3,1:1,3:2,2:1 ratio is made into mixed liquor；By cinnamic acid and fourth
Fragrant phenol is respectively with 1:3,1:6,1:9,1:1,9:1,6:1,3:1 ratio is made into mixed liquor；The mixed liquor of acquisition is cooked into antibacterial examination
It tests, observes the fungistatic effect of different proportion, the compound proportion for selecting fungistatic effect best.
The optimal proportion of the carvacrol of acquisition and Thymol and cinnamic acid and eugenol optimal proportion are pressed two kinds again to mix
The different proportion mixing for closing object, selects best antibacterial ratio.（It is fragrant：Hundred）Most preferably：（Meat：Fourth）Most preferablyCompound proportion be：1:2,1:4,
1:8,1:1,8:Isosorbide-5-Nitrae:1,2:1.
Experiment one：
1, essential oil is handled
The accurate composite essential oil for weighing 1g, is added to mixing in the conical flask with stopper containing 20g dimethyl sulfoxide (DMSO)s, is placed on ultrasonic wave
Ultrasound 15-20min on washer makes derived essential oil fully analyse and disengages.Or it is crushed with pulverizer, then ultrasound 6-8 minutes.With
Dimethyl sulfoxide (DMSO) is diluted to suitable concentration.
2, prepared by nutrient agar panel
Illustratively nutrient agar is fully dissolved with distilled water, sterilize in high-pressure sterilizing pot at 121 DEG C 20min.It has gone out bacterium
Nutrient agar is cooled to 50-55 DEG C of pour plate, be placed in 37 DEG C of insulating boxs after 12 hours after cooling take out it is spare.
3, the preparation of bacterium bacteria suspension
Picking Escherichia coli, salmonella, staphylococcus aureus single bacterium are fallen in the nutrient broth equipped with 100ml respectively, permanent
Warm shaking table culture（Overnight incubation at 37 DEG C）, it is about 10 that bacterium, which is diluted to concentration, respectively with sterile saline6-107cfu/ml
Bacteria suspension, for use.
Or 2-3 Escherichia coli of picking, salmonella, staphylococcus aureus single bacterium are fallen within containing 1ml0.9% physiology salts
In the 1.5ml centrifuge tubes of water, is fully inhaled and beaten 8-10 times with liquid-transfering gun, bacterium colony is made fully to beat, for use.
4, the preparation of filter paper
Qualitative filter paper homogeneous, that water absorbing force is strong is selected, gets the round paper of a diameter of 6mm sizes from filter paper with puncher
It is spare to be placed in super-clean bench for piece, high pressure sterilization.
5, the measurement of inhibition zone
Filter paper enzyme：It takes each 100 microlitres of the three kinds of bacteria suspensions prepared to be added on nutrient agar panel, applies bacteria stick and smoothen.It puts
After setting a period of time, grips sterilized 6mm circular filter paper pieces with aseptic nipper and be placed on nutrient agar panel, while doing 3
It is parallel, 5 microlitres of essential oil product dilutions then are drawn on filter paper with liquid-transfering gun, are put into constant incubator, are trained at 37 DEG C
It supports 12-18 hours.It observes and with the antibacterial circle diameter of each laboratory sample of vernier caliper measurement（mm）, calculate its average value.
1 carvacrol of table and the inhibition zone of Thymol different proportion compare
| Different proportion | Escherichia coli | Salmonella | S. aureus L-forms |
| 1:2 | 10 | 13 | 11 |
| 2:3 | 11 | 15 | 12 |
| 1:1 | 12 | 14 | 12 |
| 3:2 | 13 | 16 | 14 |
| 2:1 | 10 | 12 | 11 |
2 cinnamic acid of table and the inhibition zone of eugenol different proportion compare
By the above bacteriostatic test it is found that the optimum compound proportion example of carvacrol and Thymol is 3:2, cinnamic acid and eugenol
Optimum compound proportion example is 9:1.
The optimum compound proportion example that two are combined is pressed different proportion and is compounded again, and minimum inhibitory concentration method is selected to obtain best suppression
Bacterium combines.（It is fragrant：Hundred）Most preferably：（Meat：Fourth）Most preferablyCompound proportion be：1:2,1:4,1:8,1:1,8:Isosorbide-5-Nitrae:1,2:1.
Experiment two：
1, strain：Escherichia coli, salmonella, staphylococcus aureus
2, reagent：Dimethyl sulfoxide (DMSO), nutrient agar, nutrient broth
3, test apparatus and articles for use：Test tube is several or 96 orifice plates, high-pressure sterilizing pot, superclean bench, ultrasonic cleaner, mixed
Even device, constant incubator, constant-temperature table, electric heating constant-temperature blowing drying box
4, test procedure
4.1, composite essential oil is handled
The accurate composite essential oil for weighing 1.28g, is added to mixing in the conical flask with stopper containing 100ml dimethyl sulfoxide (DMSO)s, is placed on
Ultrasound 15-20min on ultrasonic cleaner makes derived essential oil fully analyse and disengages.Or it is crushed with pulverizer, then ultrasound 6-8
Minute.It is diluted to suitable concentration with dimethyl sulfoxide (DMSO).
4.2, the preparation of bacterium bacteria suspension
Picking Escherichia coli, salmonella, staphylococcus aureus single bacterium are fallen in the nutrient broth equipped with 100ml respectively, permanent
Warm shaking table culture（Overnight incubation at 37 DEG C）, it is about 10 that bacterium, which is diluted to concentration, respectively with nutrient broth6The bacterium of cfu/ml is outstanding
Liquid, for use.
4.3, it is loaded
It is that 1.28g/100ml essential oil smaples solution is added in the following order that will handle above：96 orifice plates are kept flat, in each hole
100 μ l essential oils are mixed and are added in first test tube, mixed well by the nutrient broth for adding 100 μ l, and 1ml mixed liquors is taken to be added
Into second test tube, and so on, last pipe discards.Essential oil concentration is：6400 μ g/ml, 3200 μ g/ml, 1600 μ g/
Ml, 800 μ g/ml, 400 μ g/ml, 200 μ g/ml, 100 μ g/ml, 50 μ g/ml, 25 μ g/ml, 12.5 μ g/ml.Afterwards in each test tube
1ml meat soup bacterium solutions are added, test tube is placed on 37 DEG C of incubators and stays overnight, and observes result.+ 100 μ l battalion of 100 μ l essential oils mixed liquor is done simultaneously
Support meat soup ,+100 μ l meat soup bacterium solutions of 100 μ l nutrient broths.
Table 3（It is fragrant：Hundred）Most preferably：（Meat：Fourth）The inhibition zone comparison of best difference compound proportion
| Compound proportion | Escherichia coli | Salmonella | Staphylococcus aureus |
| 1:2 | 400 | 200 | 200 |
| 1:4 | 200 | 200 | 100 |
| 1:8 | 200 | 100 | 50 |
| 1:1 | 100 | 50 | 100 |
| 8:1 | 100 | 200 | 400 |
| 4:1 | 100 | 100 | 200 |
| 2:1 | 200 | 100 | 200 |
The compound proportion of three kinds of pathogenic bacteria of composite essential oil pair is most preferably as can be drawn from Table 3：1:1.
With（It is fragrant：Hundred）Most preferably：（Meat：Fourth）Most preferablyRatio be 1:1 composite essential oil is product one；With（It is fragrant：Hundred）Most preferably：（Meat：
Fourth）Most preferablyRatio be 1:Product two is made in 1 composite essential oil after being mixed with pungent capric acid, lactic acid, fennel oil and perilla herb oil；Using etc.
The product one of amount does bacteriostatic test with product two by the method for experiment one.Its inhibition zone comparison such as table 4.
4 bacteriostatic test comparing result of table
| Different proportion | Escherichia coli | Salmonella | S. aureus L-forms |
| Product one：（It is fragrant：Hundred）Most preferably：（Meat：Fourth）Most preferably=1:1 | 19 | 23 | 20 |
| Product two：（It is fragrant：Hundred）Most preferably：（Meat：Fourth）Most preferably=1:1+ fennel oils：Perilla herb oil+pungent capric acid：Lactic acid mixed liquor | 26 | 29 | 26 |
It is learnt through testing above, compounds carvacrol, Thymol, cinnamic acid, eugenol, fennel oil, perilla herb oil by a certain percentage
And composite essential oil product made from pungent capric acid and lactic acid has stronger fungistatic effect.
Experiment three
The influence of test compound essential oil product dialogue plumage meat chicken growth performance.Experimental animal：AA white meat-type chickens.Grouping：Control group,
Test 1 group and 2 groups of experiment.
Control group：Wild marjoram perfume (or spice) phenol and any antibiotic are not added, and vaccine is used by normal procedure.
Test 1 group：Common essential oil group, carvacrol and Thymol ratio are 1:1, total effective content is 12%, and No. 1 material adds
Dosage is 350ml/T complete diet pellets, and 2, No. 3 material additive amounts are 300ml/T complete diet pellets, and whole process uses.Vaccine is used by normal procedure.
Test 2 groups：Composite essential oil group, carvacrol and Thymol ratio are 3:2, cinnamic acid and eugenol ratio are 9:1,
Fennel oil and perilla herb oil ratio are 1:1, total effective content is 1.2%, and No. 1 material additive amount is 350ml/T complete diet pellets, and 2, No. 3 material add
Dosage is 300ml/T complete diet pellets, and whole process uses.Vaccine is used by normal procedure.
Test period：- 40 age in days of 9 age in days, totally 32 days.
5 test result of table
From table 5 it can be appreciated that experiment 2 groups in white meat-type chickens feed add composite essential oil after, feedstuff-meat ratio, diarrhea rate and
The death rate is all significantly improved.Illustrate that the present invention, as synergist and chelating agent, makes by rational proportion plus pungent capric acid and lactic acid
The antibacterial ability of composite essential oil greatly improves, and effectively improves growth of animal performance, and composite essential oil is made to be substituted as feeding antibiotic
Product can be promoted and applied preferably.
The present invention operation principle be：The ratio of carvacrol and Thymol is about 3:Fungistatic effect is best when 2；Eugenol
Ratio with cinnamic acid is about 1:Fungistatic effect is best when 9.Two groups of cooperation bactericidal effects are more preferably；Fennel oil and purple are added in formula
Soviet Union's oil also has the function of flavouring, improves appetite other than enhancing bactericidal effect；This formula obtains the best of essential oil by experiment
Antibacterial ratio greatly improves product fungistatic effect.The pungent capric acid and lactic acid for adjusting acidity are added in formula, can be provided
H+, pH value is reduced, the hydrophobicity of essential oil is maintained, improves its antibacterial ability；Energy chelated metal ions simultaneously avoid it from making essential oil
With the destruction of group.Add a certain amount of TBHQ（Tert-butylhydroquinone）, the oxidation of derived essential oil is reduced, ensures the anti-of essential oil
Bacterium activity.A certain amount of neotame solution is added, the pungent mouthfeel of essential oil is reduced, improves the palatability of product.Step of the present invention（3）It adopts
With low-melting mixing, prevent derived essential oil from volatilizing and aoxidizing.It selects palm oil as capsulating material in the present invention, plays antioxygen
The effect for changing anti-volatilization keeps composite essential oil easily stored.It under the action of lipase through that can make essential oil molecule slow in enteron aisle
Release, plays the role of Continuous sterilization.
The better embodiment of this patent is explained in detail above, but this patent is not limited to above-mentioned embodiment party
Formula, one skilled in the relevant art within the scope of knowledge, can also be under the premise of not departing from this patent objective
Various changes can be made.
Claims (10)
1. a kind of Efficient antibacterial composite essential oil, which is characterized in that the Efficient antibacterial composite essential oil includes following components：Sheep's-parsley
Phenol, Thymol, cinnamic acid, eugenol, fennel oil, perilla herb oil, pungent capric acid, lactic acid, tert-butylhydroquinone, neotame solution,
White carbon, palm oil.
2. Efficient antibacterial composite essential oil according to claim 1, which is characterized in that the weight ratio of pungent capric acid and lactic acid is 3:
1, the total weight of pungent capric acid and lactic acid accounts for the 4% of Efficient antibacterial composite essential oil.
3. Efficient antibacterial composite essential oil according to claim 1, which is characterized in that the weight ratio of carvacrol and Thymol
It is 3:2；The weight ratio of eugenol and cinnamic acid is 1:9.
4. Efficient antibacterial composite essential oil according to claim 1, which is characterized in that carvacrol, Thymol total weight
It is 1 with the ratio between the total weight of eugenol, cinnamic acid:1.
5. Efficient antibacterial composite essential oil according to claim 1, which is characterized in that the weight ratio of fennel oil and perilla herb oil is
1:1, the total weight of fennel oil and perilla herb oil accounts for the 4% of Efficient antibacterial composite essential oil.
6. Efficient antibacterial composite essential oil according to claim 1, which is characterized in that the neotame solution uses neotame and nothing
Water-ethanol is according to 1：50 weight ratio mixes.
7. Efficient antibacterial composite essential oil according to claim 1, which is characterized in that the Efficient antibacterial composite essential oil includes
Following components：1.5-6 parts of carvacrol, 1-4 parts of Thymol, 2.25-9 parts of cinnamic acid, 0.25-1 parts of eugenol, fennel oil 0.5-
2 parts, 0.5-2 parts of perilla herb oil, capric acid 0.75-3 parts pungent, 0.25-1 parts of lactic acid, 0.01-0.04 parts of tert-butylhydroquinone, neotame
0.35-1.4 parts of solution, 5.49-21.96 parts of white carbon, 37-148 parts of palm oil.
8. Efficient antibacterial composite essential oil according to claim 1, which is characterized in that the Efficient antibacterial composite essential oil includes
Following components：It is 3 parts of carvacrol, 2 parts of Thymol, 4.5 parts of cinnamic acid, 0.5 part of eugenol, 1 part of fennel oil, 1 part of perilla herb oil, pungent
1.5 parts of capric acid, 0.5 part of lactic acid, 0.02 part of tert-butylhydroquinone, 0.7 part of neotame solution, 10.98 parts of white carbon, palm oil 74
Part.
9. a kind of preparation method according to any Efficient antibacterial composite essential oils of claim 1-8, which is characterized in that including
Following steps：（1）Carvacrol, Thymol, cinnamic acid, eugenol, fennel oil, perilla herb oil are added to stir in reaction kettle and mixed
It closes, it is fully spare after dissolving mixing；（2）Then pungent capric acid, lactic acid, tert-butylhydroquinone, neotame solution are added sequentially to
It is stirred in reaction kettle；White carbon is put into after mixing and adsorbs mixing in reaction kettle rapidly；（3）First palm oil is melted in advance
Change, is then then added in reaction kettle and is sufficiently mixed uniformly；Spraying apparatus is used later, and the above mixed liquor is sprayed to closed cooling
In device, cooling fine particle is formed, is sieved to get finished product.
10. the preparation method of Efficient antibacterial composite essential oil according to claim 9, which is characterized in that include the following steps：
（1）Carvacrol, Thymol, cinnamic acid, eugenol, fennel oil, perilla herb oil are added in reaction kettle and are stirred, reaction kettle
Temperature is 50 DEG C, speed of agitator 40rpm/min, fully spare after dissolving mixing；（2）Then by pungent capric acid, lactic acid, tertiary butyl
Hydroquinone, neotame solution are added sequentially in reaction kettle, and 5min is stirred under the rotating speed of 40rpm/min；It will after mixing
White carbon, which is put into, adsorbs rapidly mixing in reaction kettle；（3）First palm oil is melted at a temperature of 70 DEG C in advance, is then added
Into reaction kettle, it is sufficiently mixed at 50 DEG C uniformly；Spraying apparatus is used later, and the above mixed liquor is sprayed to closed cooling device
It is interior, cooling fine particle is formed, is sieved to get finished product.
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