CN106728000A - A kind of macleaya cordata baikal skullcap root composition and its application for veterinary drug or medicated feed additive - Google Patents
A kind of macleaya cordata baikal skullcap root composition and its application for veterinary drug or medicated feed additive Download PDFInfo
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- CN106728000A CN106728000A CN201611044294.3A CN201611044294A CN106728000A CN 106728000 A CN106728000 A CN 106728000A CN 201611044294 A CN201611044294 A CN 201611044294A CN 106728000 A CN106728000 A CN 106728000A
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- macleaya cordata
- skullcap root
- veterinary drug
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/66—Papaveraceae (Poppy family), e.g. bloodroot
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
Abstract
The invention discloses a kind of macleaya cordata baikal skullcap root composition and its application for veterinary drug or medicated feed additive；The macleaya cordata baikal skullcap root composition includes macleaya cordata ground non-fruit part (containing macleaya cordata aerial part and Post flowering macleaya cordata ground non-fruit part before blooming) and/or macleaya cordata ground non-fruit extracting section thing, and the root of large-flowered skullcap and/or Baical Skullcap root P.E；Said composition is applied to prepare anti-inflammatory, antibacterial, resisting stress, the veterinary drug of pre- anti-diarrhea, or for preparing the medicated feed additive for promoting growth of animal or improving food conversion or improvement growth of animal performance；Veterinary drug or medicated feed additive with preferable resisting stress, anti-inflammatory, protection intestinal health and improve growth of animal performance to treatment animal alimentary canal diseases and mastitis for milk cows good effect；Composition material source is wide, low cost, realizes comprehensive utilization of resources, is worthy of popularization.
Description
Technical field
The present invention relates to a kind of macleaya cordata (Macleaya cordata (Willd) R.Br.) and the root of large-flowered skullcap (Scutellaria
Baicalensis Georgi) composition, wherein macleaya cordata include macleaya cordata and macleaya microcarpa Fedde (Macleaya
Microcarpa (Maxim.) Fedde), the non-fruit in more particularly to a kind of macleaya cordata ground non-fruit part and/or macleaya cordata ground
Real part point extract, and the application of the root of large-flowered skullcap and/or Baical Skullcap root P.E composition in veterinary drug or animal pharmaceuticals feed addictive,
Belong to field of veterinary.
Background technology
The abuse of aquaculture feeding antibiotic, have impact on nature microbial environment in a balanced way, cause the adaptability of cause of disease
Variation, causes bacterial drug resistance to increase, and also causes the decline of animal autoimmunity, disease to take place frequently and feed intake reduction.
Food-safety problem caused by feeding antibiotic has received the extensive concern of countries in the world government, numerous and confused vertical
Method forbids being used antibiotic as feed addictive, and the national strict limitation of also making laws without disabling is stopped using simultaneously strict regulations
The medicine phase.
European Union completely forbade antibiotic and is used as feed addictive in 2006；2012 end of the year U.S. FDAs come into effect
Forbid being added in feed the decree of antibiotic；China starts to phase out or limit the use of feeding antibiotic.Use green
The natural drug additive substitute antibiotics feed addictive of safety, is the inexorable trend of Modern Animal Husbandry development.
The bioactivity such as herbal medicine source crude drug, polysaccharide, saponins, alkaloids, volatile oil that it contains
Material；Bioactivator exists for a long time in nature, and certain balance is adapted to and established with microorganism, is not easily caused bacterium
Drug resistance produce；Bioactivator can voluntarily degrade in the environment, be unlikely in agricultural products, livestock products long-term residual
Stay or be enriched with by food chain, the irreversible breaking to environment is not caused, to people and animals with respect to low toxicity, meet green ecological animal husbandry
Requirement to medicine, is the important development direction of following veterinary drug.Using herbal medicine product, animal health, food are not only ensure that
Safety, and be preferable feeding antibiosis with increasing body's immunity, promoting various functions such as growth of animal, improvement meat
Plain substitute.
There is extremely abundant plant and natural products resource in China, new antibiotic is found from natural products resource and is substituted
Material, with vast potential for future development.Macleaya cordata Macleaya cordata (Willd.) R.Br., China's most area is all
It is distributed, also known as horn grass, horn bar, bubble cylinder bar, Greensboro time, it is a kind of folk tradition Chinese medicine rich in alkaloid, it is opium poppy
The herbaceos perennial of section's Macleaya.Totally two kinds of full category, including macleaya cordata and macleaya microcarpa Fedde (Macleaya
microcarpa(Maxim.)Fedde).Macleaya cordata is very long in medicinal history among the people, first recorded in《Bencao Shiyi》、《Herbal guiding principle
Mesh》、《Dictionary of medicinal plant》And《Chinese medicinal herbal》Deng Chinese medicine ancient books and records.In traditional ancient hospital, macleaya cordata is often entered with dry herb
Medicine.
The Macleaya cordata injection included in the application aspect of macleaya cordata, Ministry of Agriculture's veterinary medical quality standard be by macleaya cordata really
Sterile water solution that is extracted, separating the biological alkali sulfate being made, it has the function of anti-inflammation, cures mainly the white of piglet
Dysentery, yellow dysentery；Herbal medicine macleaya cordata extracts and its preparation be by macleaya cordata Fruit pod it is extracted, separate the benzo phenanthridines class that is made and give birth to
Alkaloids, with anti-inflammation, growth promotion (patent No. ZL200910043145.9)；
Yu Jiansheng (2007) carries active ingredient TA extracted in macleaya cordata Fruit pod and root and Herba Andrographitis herb
Take to andrographolide and be made Macleaya cordata injection, oral liquid, be used to treat the white scour of piglet, the preferable effect of cholera fowl disease acquirement
Really；Macleaya cordata and Herba Andrographitis cooperation are made Macleaya cordata injection patent applied for (patent No. ZL by Yu Jiansheng etc.
200510020409.0), it has wide spectrum clearing heat, releasing toxin, removing swelling, anti-bacteria and anti-virus anti-inflammatory effect, is mainly used in treatment livestock and poultry and suffers from
Influenza, diarrhoea dysentery, the white scour of piglet and yellow dysentery etc.；
Once was founded the state and macleaya cordata medicinal material is carried out diacolation with acid solution, percolate added alkali to be adjusted to meta-alkalescence and carry out alkali to sink, and sank
Shallow lake low-carbon alcohols dissolve, and add the appropriate activated carbon to carry out refluxing extraction, and acid adding is into saltouing after extract solution recovery section solvent
Go out salmon pink crystalline precipitate, precipitation filtration alcohol wash away residual acid dry after obtain final product macleaya cordata extracts patent applied for (specially
Profit ZL 200910043045.6).
Once founded the state is used to preparing to livestock and poultry encountered pathogenic bacteria Li bacillus, bloodthirsty by macleaya cordata Fruit pod and/or leaf extract
Bacillus, Pasteurella or streptococcus have the veterinary drug of good inhibiting effect and killing action；Or be used to macleaya cordata extracts make
The veterinary drug patent applied for (patent No. ZL 2,010,102,930 39.9) of standby pre- anti-diarrhea effect.
Once founded the state from plant --- macleaya cordata, macleaya microcarpa Fedde, snowpoppy, greater celandine containing benzylisoquinoline alkaloid
The protopine total Alkaloid for obtaining is extracted in plant, mainly with Biflorine and allocryptopine to represent patent applied for
(patent No. ZL 200910042840.3), it has good application in terms of economic animal pig, chicken nematodiasis is driven away.
The content of the invention
In prior art more than it can be appreciated that at present existing technical staff research substantially concentrate on respectively it is rich
The extraction and application for dropping back into total alkaloid content highest Fruit pod or fruit are upper；Research to the other parts of macleaya cordata, especially
It is how the macleaya cordata of the part carries out the fresh understatement road of compatibility with other medicinal materials.
Inventor is by lasting research of many decades to macleaya cordata, pleasantly surprised discovery, in macleaya cordata ground non-fruit part
Alkaloid it is relatively low in the case of, macleaya cordata ground non-fruit part and its extract are matched somebody with somebody with the root of large-flowered skullcap or its extract
5, the effect with good Synergistic.Inventor research find said composition have to animal good anti-inflammatory, it is antibacterial,
Resisting stress and anti-diarrhea effect, and promote growth of animal, improve food conversion ratio, improve breeding performonce fo animals or protection enteron aisle
Health.Macleaya cordata ground non-fruit part or its extract coumaric acyl containing N-P- tyrasamine composition, content in 1.0~100.0ppm,
It is used for veterinary drug or animal pharmaceuticals feed addictive by the present invention with the root of large-flowered skullcap or its extract compatibility has good technique effect,
Be inventor do not expect completely.
On the basis of existing technology, the purpose of the present invention is intended to improve a kind of for veterinary drug or medicated feed additive
Macleaya cordata-baikal skullcap root composition, by macleaya cordata ground non-fruit part and/or macleaya cordata ground non-fruit extracting section thing and the root of large-flowered skullcap
And/or fresh Scutellaria baicalensis extract combination is used, there is anti-inflammatory, antibacterial, resisting stress and anti-diarrhea, can make as veterinary drug
With；Meanwhile, the good effects also with promotion growth of animal or raising food conversion or improvement growth of animal performance, or can make
For animal pharmaceuticals feed addictive is used.
It is to provide a kind of application of macleaya cordata-baikal skullcap root composition that another object of the present invention is, said composition is in system
The veterinary drug of standby anti-inflammatory, antibacterial, resisting stress and anti-diarrhea, or prepare and promote growth of animal or improve food conversion ratio or improve animal
Production performance, is respectively provided with good application effect in regulating intestinal canal flora medicated feed additive, higher with drug effect, residual
The advantages of low, Small side effects, it is more beneficial for economizing on resources.
In order to realize above-mentioned technical purpose, the invention provides it is a kind of for veterinary drug or medicated feed additive it is rich fall
Return-baikal skullcap root composition, the macleaya cordata-baikal skullcap root composition it is non-on the ground including macleaya cordata ground non-fruit part and/or macleaya cordata
Fruit parts extract, and the root of large-flowered skullcap and/or Baical Skullcap root P.E.
Preferred scheme, macleaya cordata ground non-fruit part include blooming before macleaya cordata aerial part and/or Post flowering it is rich
Drop back into ground non-fruit part.
More preferably scheme, N-P- coumaric acyls tyramine content is 1~100ppm in macleaya cordata-baikal skullcap root composition, more preferably
It is 1~50.0ppm；General flavone content is 1~10000ppm, is more preferably 1~6000ppm.
More preferably scheme, described macleaya cordata ground non-fruit part is made macleaya cordata powder by drying, crushing；
The described root of large-flowered skullcap is made Baical Skullcap Root by drying, crushing；Described macleaya cordata ground non-fruit part is molten by after drying
Agent is extracted, and is made macleaya cordata extracts；The described root of large-flowered skullcap is by after drying, solvent extraction obtains Baical Skullcap root P.E；Will be described rich
Drop back into powder and/or the macleaya cordata extracts mix with the Baical Skullcap Root and/or the Baical Skullcap root P.E, obtain final product and win
Return-baikal skullcap root composition.
More preferably scheme, macleaya cordata ground non-fruit part is by after drying, solvent extraction is made macleaya cordata extraction
Thing.In extraction process, the solvent for using is alcohols (such as methyl alcohol, ethanol) or acid solution (such as sulfuric acid solution).Scutellariae,radix passes through
After drying, Baical Skullcap root P.E is made by techniques such as solvent extractions.In extraction process, the solvent for using is alcohols (such as methyl alcohol, second
Alcohol etc.) or the aqueous solution.
More preferably scheme, the specific extracting method of macleaya cordata ground non-fruit extracting section thing：It is non-on the ground with macleaya cordata
Fruit parts are extracted by sour water (such as sulfuric acid solution), regulation pH value is 8~9, and after staticly settling, gained precipitation uses alcoholic solution again
Extract, concentration, with the heavy salt of concentrated acid, obtain macleaya cordata extracts.
In macleaya cordata-baikal skullcap root composition of the invention, the ratio of macleaya cordata ground non-fruit amount of powder and Baical Skullcap Root
Have 1:1、2:1、3:1 and 4:1, addition of the active principle composition in feed is ensured in feed addictive, preferably win
Return-baikal skullcap root composition addition at 0.1Kg/ tons~4.0Kg/ tons, further more preferably scope is at 0.5Kg/ tons~1.5Kg/ tons
Addition.
Present invention also offers the application of described macleaya cordata-baikal skullcap root composition, macleaya cordata-baikal skullcap root composition is used to make
The veterinary drug of standby anti-inflammatory, antibacterial, resisting stress and anti-diarrhea；Or promote growth of animal, improve food conversion ratio, improve for preparing
The animal pharmaceuticals feed addictive of breeding performonce fo animals or regulating intestinal canal flora.
Preferred scheme, macleaya cordata-baikal skullcap root composition is used to prepare to gram-positive bacteria and/or Gram-negative bacteria
There is the veterinary drug of inhibitory action；Or for preparing the veterinary drug for the treatment of animal alimentary canal diseases, anti-mastitis for milk cows, resisting stress.
Preferred scheme, macleaya cordata-baikal skullcap root composition is used to prepare to including Staphylococcus gallinarum, white diarrhea Salmonella
Bacterium, Salmonella choleraesuls, ETEC, and/or staphylococcus aureus have suppression or kill in interior encountered pathogenic bacteria
Go out the veterinary drug of effect.
More preferably scheme, the addition concentration of veterinary drug prepared by macleaya cordata-baikal skullcap root composition in animal feed, with N-P-
Coumaric acyl tyramine content is 0.1~300ppb and general flavone content for 1.0~100ppm is measured.Further preferred scheme, wins
Drop back into-baikal skullcap root composition when the veterinary drug of anti-inflammatory, antibacterial, resisting stress and anti-diarrhea is prepared, prepared by macleaya cordata-baikal skullcap root composition
Addition concentration of the veterinary drug in animal feed, with N-P- coumaric acyls tyramine content as 0.5~100ppb and general flavone content be 1~
80ppm is measured；It is 1~70ppm meterings more preferably by 1~70ppb and general flavone content of N-P- coumaric acyls tyramine content.
More preferably scheme, the addition of medicated feed additive prepared by macleaya cordata-baikal skullcap root composition in animal feed
Concentration, is 1.0~50.0ppm meterings by 1.5~60ppb and general flavone content of N-P- coumaric acyls tyramine content.It is further excellent
The scheme of choosing, macleaya cordata-baikal skullcap root composition in the promotion growth of animal for preparing, improve food conversion ratio, improve animal productiong
During the animal pharmaceuticals feed addictive of energy or regulating intestinal canal flora, the addition concentration in animal feed, with N-P- coumaric acyl junket
Amine content is 2.7~37.5ppb and general flavone content for 1.0~45.0ppm is measured；More preferably contained with N-P- coumaric acyl tyrasamines
Measure is that 4.1~18.8ppb and general flavone content are 1.0~40.0ppm meterings.
Preferred scheme, macleaya cordata-baikal skullcap root composition as veterinary drug or animal pharmaceuticals to raise in the form of powder or pre-mixing agent
Feed additives are used.
More preferably scheme, veterinary drug or animal pharmaceuticals feed addictive are mixed with feed and feed oral mode and be administered.
Macleaya cordata-baikal skullcap root composition of the invention directly can be administered with active compound powder in the form of mixed feeding.And macleaya cordata-Huang
The extract included in a kind of reed mentioned in ancient books composition can add auxiliary material be made the preparations such as pre-mixing agent or powder mix feed be administered orally.
Technical scheme using macleaya cordata ground non-fruit part former powder and/or extract and the root of large-flowered skullcap and/or
Baical Skullcap root P.E (preferably fresh Scutellaria baicalensis) is combined, and is applied to anti-inflammatory, antibacterial, resisting stress and anti-diarrhea, or promote growth of animal
Or raising food conversion or improvement growth of animal performance have good effect.
The macleaya cordata ground non-fruit part tyrasamine of coumaric acyl containing N-P- that technical solution of the present invention is used, and in the prior art
Medicinal part be macleaya cordata really.The present invention wins after the April before macleaya cordata is bloomed can tap ground non-fruit part
Returning can be rebloomed result with continued growth, increase the income of plantation peasant.And the main active in the root of large-flowered skullcap is to include the root of large-flowered skullcap
Glycosides in interior Flavonoid substances, with anti-inflammatory and immunoregulation effect.Inventor is had found by long-term practice and research will be rich
Drop back into (N-P- coumaric acyls tyrasamine) and the root of large-flowered skullcap (scutelloside) is combined and can prepare with anti-inflammatory, antibacterial, resisting stress and anti-abdomen
Rush down the veterinary drug of effect, prepare to have and promote growth of animal or improve food conversion ratio or improve breeding performonce fo animals, regulating intestinal canal
The medicated feed additive of flora effect, and higher with drug effect, the advantages of remain low, Small side effects, is conducive to economizing on resources.
Be combined for macleaya cordata and the root of large-flowered skullcap by technical scheme, make full use of the natural effective active that it is included into
Point, animal alimentary canal diseases are treated, regulating intestinal canal flora, resisting stress improves growth of animal performance etc., can replace existing anti-
Raw element etc. is used.
To prior art, the Advantageous Effects that technical scheme is brought：
Used as active component present invention firstly provides using the dried powder or extract of ground non-fruit part and the root of large-flowered skullcap
In the veterinary drug for being made anti-inflammatory, antibacterial, resisting stress and anti-diarrhea, or for preparing what is promoted growth of animal and improve food conversion ratio
Animal pharmaceuticals feed addictive, with good drug efficacy, the advantage of Small side effects can replace existing associated antibiotic and use.
Ground non-fruit part and the root of large-flowered skullcap that the present invention is used, belong to natural plant composition, and source is wide, easily obtains,
And can be remained less with natural degradation, it is low to people and animals' toxicity, meet green ecological animal husbandry standard.
Specific embodiment
Following examples are intended to further illustrate present invention, rather than the protection model of limitation the claims in the present invention
Enclose.
The N-P- coumaric acyl tyramine contents of macleaya cordata ground non-fruit part dry powder or extract are examined by HPLC methods
Survey, the active component content in dry powder or extract is calculated with N-P- coumaric acyls tyramine content.The root of large-flowered skullcap/fresh Scutellaria baicalensis extract
General flavone content detected by ultraviolet or HPLC methods, the root of large-flowered skullcap/Baical Skullcap root P.E general flavone is calculated with content of baicalin
Content.The macleaya cordata ground non-fruit part used in embodiment refer to bloom before macleaya cordata aerial part and Post flowering macleaya cordata ground
Upper non-fruit part.
Embodiment 1
This experiment is by macleaya cordata ground non-fruit amount of powder (containing macleaya cordata aerial part and Post flowering macleaya cordata before blooming
Non-fruit part) or its extract be combined with root of large-flowered skullcap powder or its extract, according to compared with ratio of greater inequality example prescription, then survey its combination
The content of N-p- coumaric acyls tyrasamine and scutelloside in thing, each prescription ratio and content range see the table below 1
The content range of each prescription N-p- coumaric acyls tyrasamine of table 1 and scutelloside
Embodiment 2
Macleaya cordata extracts, Baical Skullcap root P.E and macleaya cordata-root of large-flowered skullcap combined extracts press down in vitro to livestock and poultry encountered pathogenic bacteria
System experiment.
From macleaya cordata extracts (N-p- coumaric acyls tyrasamine 0.01%), Baical Skullcap root P.E (scutelloside 1%) and macleaya cordata-
Baical Skullcap root P.E (N-p- coumaric acyls tyrasamine 0.01%, scutelloside 1%), extract is configured to the solution of isoconcentration, then right respectively
The livestock and poultry such as Staphylococcus gallinarum, S. pullonum, Salmonella choleraesuls, ETEC and staphylococcus aureus are normal
The animal pathogen seen carries out extracorporeal bacteria inhibitor test.By sterile working code, aseptically, corresponding strain correspondence is toppled over
Culture medium flat plate, the corresponding strain inoculum of coated thereon, after flat board solidification after, paste dosing susceptability test paper piece, use aseptic nipper
It is tightly combined scraps of paper peace plate.Every part of sample does 3 parts, results averaged.37 DEG C are inverted culture, and observe antibacterial in 24h
Effect, determines the diameter of inhibition zone, judges susceptibility.
Criterion：Inhibition zone diameter is judged as there is bacteriostasis more than 7mm person, and inhibition zone diameter is less than or equal to 7mm
Without bacteriostasis, bacteriostatic diameter is bigger, and explanation fungistatic effect is better.
Result detection shows：The concentration of the extract of macleaya cordata extracts, Baical Skullcap root P.E and macleaya cordata-root of large-flowered skullcap is
Under the conditions of 100 μ g/mL, Staphylococcus gallinarum, S. pullonum, Salmonella choleraesuls, ETEC and golden yellow
The bacteriostatic diameter of 5 kinds of livestock and poultry encountered pathogenic bacterias of staphylococcus is all higher than 7mm, shows good bacteriostasis (being shown in Table 2).
Influence (unit of each extract of table 2 to bacteriostatic diameter：mm)
Using tube dilution method, measure has the minimum inhibitory concentration (MIC) of the liquid of bacteriostatic activity.Macleaya cordata extracts,
Baical Skullcap root P.E and macleaya cordata-Baical Skullcap root P.E are test group, and every group takes sterile test tube 13, is arranged in a row, except the 1st pipe is added
The 1.8mL MH bacterium solutions of 1000 times of broth dilution, remaining often pipe add MH meat soup 1mL, be separately added into every group of the 1st pipe and win
Return the solution stoste 0.2mL that extract, Baical Skullcap root P.E and macleaya cordata-Baical Skullcap root P.E prepare to mix, then draw 1mL to the
2 manage, and draw the pipes of 1mL to the 3rd after mixing again, and so continuous doubling dilution is to the 12nd pipe, and absorption 1mL is discarded from the 12nd pipe,
13rd pipe is the growth control of not drug containing.37 DEG C of culture 24h, are minimal inhibitory concentration with the least concentration without bacterial growth
(MIC)。
The testing result of minimal inhibitory concentration shows：Macleaya cordata extracts, Baical Skullcap root P.E and macleaya cordata-Baical Skullcap root P.E
To Staphylococcus gallinarum, S. pullonum, Salmonella choleraesuls, ETEC and the breeding stock of staphylococcus aureus 5
Fowl encountered pathogenic bacteria strain has a good fungistatic effect, i.e., have good suppression to Gram-positive and Gram-negative bacteria
Bacterium effect；But the fungistatic effect of macleaya cordata-Baical Skullcap root P.E prescription is extracted significantly better than single macleaya cordata extracts or the root of large-flowered skullcap
Thing.The medicine of its antibiotic as an alternative, its source is natural, degraded is fast, it is low to remain, Small side effects the advantages of, good market
Prospect.
Minimal inhibitory concentration MIC (the units of each extract of table 3：μg/mL)
Embodiment 3
Macleaya cordata-root of large-flowered skullcap group extract (N-p- coumaric acyls tyrasamine 0.01%, scutelloside 1%) causes mouse abdomen to Escherichia coli
The influence rushed down
ICR mouse 40, male and female half and half are randomly divided into 5 groups, respectively every group 8, model pair by sex, body weight district's groups
According to group, Salazosulfadimidine group, the basic, normal, high dosage group of macleaya cordata-Baical Skullcap root P.E, each group mouse is given by 0.2mL/10g gavages
Corresponding liquid, one time a day, successive administration 3 days, and model control group mouse gives isometric solvent 0.5% by same procedure gavage
CMC-Na.30min before last dose, each group mouse presses 0.6mL/ only, intraperitoneal injection 6 × 109The Escherichia coli solution of/mL, note
Penetrate rear Continuous Observation 4h, passage of loose stools time, diarrhea of mouse rate and Scours index in 4h first after record intraperitoneal injection Escherichia coli.
The mouse single of table 4 gavages medicine-feeding test dosage
Testing index：
Each group diarrhea of mouse latent time：The time of intraperitoneal injection Escherichia coli to occur first diarrhoea time interval.
Each group diarrhea of mouse rate：The number of animals of diarrhoea/this group of animal number × 100%.
Each group diarrhea of mouse index in 4h：Scours index=loose stool rate × average loose stools level.Loose stool rate is every dynamic in formula
The loose stools number that thing is arranged with always just number the ratio between.Loose stools level represents every degree of animal loose stools, and the diameter of filter paper is polluted with loose stools
Deciding grade and level, is divided into 4 grades：It is l grades less than 1cm, 1~1.9cm is 2 grades, 2~3cm is 3 grades, is 4 grades more than 3cm.Average loose stools level=
All loose stools level/loose stools number of times.
Result see the table below 5：
Table 5 causes the test of pesticide effectiveness result of Diarrhea Model to Escherichia coli
Note：Compare with model control group, digital right subscript " * ", represent significant difference (P<0.05)
The result of table 5 shows, the high, medium and low dosage group Scours index of macleaya cordata Baical Skullcap root P.E compared with control group significant difference, and
There is significant difference (P<0.05), macleaya cordata-Baical Skullcap root P.E has good therapeutic action to bacterial diarrhea model.
Embodiment 4
Shadow of the pre-mixing agent that macleaya cordata powder, root of large-flowered skullcap powder and macleaya cordata-baikal skullcap root composition are respectively prepared to piglet growth performance
Ring experiment
Experimental design：Two-way cross sodium selenite 250 is chosen, 25 ages in days or so are randomly divided into 5 groups, male and female half and half.Examination
Test and be grouped as follows：
The experiment packet of the piglet growth performance of table 6
By plant's standardization program feeding and management.Other medicines feed addictive, free water are free of in feeding daily ration.Examination
The cycle is tested for 30d.
Record index：Average daily gain (ADG), average daily gain (ADFI), feed conversion rate (FCR).
The piglet growth performance result of the test of table 7
As understood in table 7, compared with negative control group, addition macleaya cordata powder, root of large-flowered skullcap powder and macleaya cordata-root of large-flowered skullcap experimental group
Piglet average daily gain ratio has been respectively increased 1.6%, 1.7% and 3.1%, and macleaya cordata is compared with addition aureomycin group (2.1%)
The feed intake of root of large-flowered skullcap powder group increased；Daily gain has been respectively increased 6.5%, 8.7% and 17.5%, better than aureomycin group
(10.9%)；The feed conversion rate of macleaya cordata-baikal skullcap root composition experiment significant difference (p compared with control group<0.05), and significantly
Reduce feedstuff-meat ratio.Result shows：Feed adds macleaya cordata-baikal skullcap root composition pre-mixing agent and is better than feed to the growth performance of piglet
Addition macleaya cordata or root of large-flowered skullcap pre-mixing agent.
Embodiment 5
Macleaya cordata-baikal skullcap root composition powder (N-p- coumaric acyl tyrasamines 30ppm, scutelloside 60ppm) is to mastitis for milk cows
Therapeutic action
Experimental design：120 are suffered from mammitis milk cow, wherein, raise 6 months ages~5 year, the state of an illness 10 days~3 months.Random point
For：Treatment group 60, control group 60.Two groups of beast ages etc., there was no significant difference, with comparativity experiment.
Test method：(1) control group takes certain commercially available anti-inflammatory powder for animals, takes to specifications.The course for the treatment of 30 days.(2)
Treatment group feeds the powder of macleaya cordata-baikal skullcap root composition of the present invention, it is early, middle and late it is each once.The course for the treatment of 30 days.(3) criterion of therapeutical effect and
Treatment results：Criterion of therapeutical effect：Recovery from illness：The clinical symptoms of mastitis for milk cows all disappear.Take a turn for the better：The clinical symptoms of mastitis for milk cows
Mitigate, the state of an illness is controlled.It is invalid：No significant improvement or aggravated in clinical symptoms for mastitis for milk cows.With calculating of fully recovering and take a turn for the better
It is efficient.Treatment statistics are shown in Table 8.
8 two groups of efficacy results of table
As can be seen here, mastitis for milk cows can safely and effectively be treated using macleaya cordata of the invention-baikal skullcap root composition powder.
Embodiment 6
Macleaya cordata-baikal skullcap root composition powder (N-p- coumaric acyl tyrasamines 10ppm, scutelloside 30ppm) is to dairy cattle
Influence
Experimental design：30 2~5 months postpartum of selection, the output of milk more than 20 kilograms, health, the moderate lactation of the growth of livestock
Milk cow is matched by body weight, parity and the same or like principle of the output of milk, is divided into totally 2 groups of blank control group, test group, often
15 repetitions of group, each repeats 1, totally 30 test ox.Each group treatment is respectively, blank control group：Basal diet；Experiment
Group：Basal diet+macleaya cordata-root of large-flowered skullcap powder.
Feeding and management：Test ox concentrates on same cowshed, and raising is responsible for by special messenger.The daily TMR feedings of test ox, freely drink
Water, dissipate formula management, daily at any time observation ox appetite, ruminate, spirit, fecaluria and whether have mammitis occur etc. behavior
Situation, carries out each single item record.Milk 3 times daily, milked in milking parlour respectively.Blank control group, test group experimental period be
40d, wherein preliminary trial period 10d, formal phase 30d.
Table 9 influences on dairy cattle
As seen from the above table, the average daily galactopoiesis of test group is substantially higher than blank group, and butterfat, lactoprotein, lactose in breast
All increase accordingly with milk density, and test group is compared with the significant difference of blank group；This shows macleaya cordata-baikal skullcap root composition powder energy
Improve the lifting of the milk production and breast matter amount of milk cow.
Embodiment 7
Macleaya cordata-root of large-flowered skullcap powder (N-p- coumaric acyl tyrasamines 30ppm, scutelloside 60ppm) controls curative effect examination to dog enterogastritis
Test
Choose 50 dogs with enterogastritis, all dogs all have one or more of the common performance of dog enterogastritis, such as eat
Be intended to it is depressed, with vomiting；Stench bloody stool is discharged once in a while；Body temperature is raised, even has salivation, foam and spasm.It is randomly divided into：Medicine
Control group 25, treatment group 25.
Test method：(1) control group takes certain commercially available pet treatment dog gastroenteritis medicine, takes to specifications.The course for the treatment of 3
~5 days.(2) treatment group feeds macleaya cordata of the invention-root of large-flowered skullcap powder, it is early, middle and late it is each once.The course for the treatment of 30 days.
Criterion of therapeutical effect：(1) fully recover：Recover normal, each item data inspection recovers normal；(2) effectively：Various enterogastritis symptoms
Mitigate, each item data inspection substantially tends to normal；(3) it is invalid：To no effect or sb.'s illness took a turn for the worse.
All case efficacy results are shown in Table 10.
The all case efficacy results of table 10
As can be seen here, dog enterogastritis can safely and effectively be treated using medicine obtained in the present invention.
Embodiment 8
Macleaya cordata-influence of the baikal skullcap root composition pre-mixing agent to dorking heat-resistant stress：
Experimental design：From the white meat-type chickens of 500 1 ages in days health, 5 groups are randomly divided into, every group 100, male and female half and half,
Experiment is grouped as follows：
The experiment packet of the dorking heat-resistant stress of table 11
The selection of stressed condition：Selection month summer 7-8,35 DEG C of daytime temperature can continue 4~6 hours, belong to nature generation
The condition chicken house of heat stress.
By plant's normal farm standardization program feeding and management.Other medicines feed addictive is free of in feeding daily ration.Often
Epidemic disaster in its record hen house.Test chicken is supported using high bed is flat, and free water, scale of feeding is combined by recommendation nutritional requirements
Depending on actual feed intake.Hygienic cleaning and sterilization are carried out according to a conventional method.Experimental period, keeps crib, tank sanitation and hygiene.
Record index：Average daily gain (ADG), average daily gain (ADFI), feed conversion rate (FCR).
The dorking heat-resistant stress result of the test of table 12
Result as shown in table 12 shows：The basic, normal, high dosage of macleaya cordata baikal skullcap root composition is added in daily ration can effectively be increased
Plus the growth performance of white meat-type chickens, improve food conversion ratio, reduce feedstuff-meat ratio value.Show in this experiment, add macleaya cordata-Huang
A kind of reed mentioned in ancient books composition can enable chicken group in the condition normal health growth of heat stress and increase the yield of meat.In sum, macleaya cordata
Baikal skullcap root composition has stronger anti-heat stress effect.
Embodiment 9
Macleaya cordata pre-mixing agent (N-p- coumaric acyls tyrasamine 10ppm), root of large-flowered skullcap pre-mixing agent (scutelloside 30ppm) and macleaya cordata-Huang
Influence of a kind of reed mentioned in ancient books pre-mixing agent (N-p- coumaric acyl tyrasamines 10ppm, scutelloside 30ppm) to aquatic livestock (grass carp) production performance
Test after carrying out 1 week raise and train with fish, selection specification is consistent, physically fit, feeding is active, the disease-free cultivation without wound
Animal, is randomly divided into 4 groups, and blank control group, macleaya cordata group, root of large-flowered skullcap group and macleaya cordata-root of large-flowered skullcap group, every group of each setting 3 are put down
OK, each parallel 30 tail, test period 60d.
Test fish is raised in the net cage of 3m × 1.5m × 1.5m, and the pond waters pH for setting up net cage is 6.8, and dissolved oxygen is big
In 5mg/L, before on-test, pond is carried out into pasteurised completely, carried out disinfection after fish is transported back and packet is weighed, it is pre- to raise 1 week
Fish to be tested is tamed to the normal rear formal experiment of weighing of food.Feed 2 times daily, every morning 9：00 and afternoon 17：00 raises respectively
Feed once, fed by the 3~5% of body weight daily, throw feeding rate and determine that it is original to satisfy with 8~9 one-tenth according to body weight, water temperature condition
Then；Daily measurement water temperature, 25 DEG C~35 DEG C of water temperature during experiment.
Influence of the table 13 to Growth of Grass Carps Ctenopharyngodon Idellus effect
Note：Data are average ± standard deviation in table, have a same letter person with all after column data, represent that difference does not show
Write (P>0.05, Duncan ' s methods).
After the nursing of 60d, the growing state of grass carp is shown in Table 13；As shown in Table 13, three groups of phases of test group grass carp
Blank control group (P is significantly higher than to rate of body weight gain and average daily gain<0.05), macleaya cordata-root of large-flowered skullcap group is than macleaya cordata group, the root of large-flowered skullcap
The body weight increase rate of group test group and average daily gain are all high, and with certain otherness (P<0.05)；Three groups of test group grass
The feed coefficient of fish is substantially less than blank group (P<0.05).Therefore, macleaya cordata-Baical Skullcap root P.E is added in feed of grass carp than addition
Macleaya cordata or root of large-flowered skullcap growth-promoting effect are substantially preferable.
Claims (10)
1. a kind of for veterinary drug or the macleaya cordata-baikal skullcap root composition of medicated feed additive, it is characterised in that：Including macleaya cordata ground
Upper non-fruit part and/or macleaya cordata ground non-fruit extracting section thing, and the root of large-flowered skullcap and/or Baical Skullcap root P.E.
2. according to claim 1 for veterinary drug or the macleaya cordata-baikal skullcap root composition of medicated feed additive, its feature exists
In：Macleaya cordata aerial part and/or Post flowering macleaya cordata are non-on the ground before described macleaya cordata ground non-fruit part includes blooming
Fruit parts.
3. according to claim 1 and 2 for veterinary drug or the macleaya cordata-baikal skullcap root composition of medicated feed additive, it is special
Levy and be：In macleaya cordata-baikal skullcap root composition N-P- coumaric acyls tyramine content be 1~100ppm, general flavone content be 1~
10000ppm。
4. according to claim 1 or 2 or 3 for veterinary drug or the macleaya cordata-baikal skullcap root composition of medicated feed additive, its
It is characterised by：
Described macleaya cordata ground non-fruit part is made macleaya cordata powder by drying, crushing；
The described root of large-flowered skullcap is made Baical Skullcap Root by drying, crushing；
Described macleaya cordata ground non-fruit part is by after drying, solvent extraction is made macleaya cordata extracts；
The described root of large-flowered skullcap is by after drying, solvent extraction obtains Baical Skullcap root P.E；
The macleaya cordata powder and/or the macleaya cordata extracts are mixed with the Baical Skullcap Root and/or the Baical Skullcap root P.E
Close, obtain final product macleaya cordata-baikal skullcap root composition.
5. the application of the macleaya cordata-baikal skullcap root composition described in any one of Claims 1 to 4, it is characterised in that：
Veterinary drug for preparing anti-inflammatory, antibacterial, resisting stress or anti-diarrhea；
Or promote growth of animal, improve food conversion ratio, improve breeding performonce fo animals or protect intestinal health for preparing
Medicated feed additive.
6. the application of macleaya cordata-baikal skullcap root composition according to claim 5, it is characterised in that：
For preparing the veterinary drug for having inhibitory action to gram-positive bacteria and/or Gram-negative bacteria；
Or for preparing treatment animal alimentary canal diseases, anti-mastitis for milk cows or the veterinary drug of resisting stress.
7. the application of macleaya cordata-baikal skullcap root composition according to claim 5, it is characterised in that：
For prepare to including Staphylococcus gallinarum, S. pullonum, Salmonella choleraesuls, ETEC and/or
Staphylococcus aureus has the veterinary drug of suppression or killing action in interior encountered pathogenic bacteria.
8. the application of macleaya cordata-baikal skullcap root composition according to claim 5, it is characterised in that：Macleaya cordata-baikal skullcap root composition
Addition concentration of the veterinary drug of preparation in animal feed, is that 0.1~300ppb and general flavone contain with N-P- coumaric acyls tyramine content
Measure as 1.0~100.0ppm is measured.
9. the application of macleaya cordata-baikal skullcap root composition according to claim 5, it is characterised in that：Macleaya cordata-baikal skullcap root composition
Addition concentration of the medicated feed additive of preparation in animal feed, with N-P- coumaric acyls tyramine content as 1.5~60ppb and
General flavone content is measured for 1.0~50.0ppm.
10. the application of macleaya cordata-baikal skullcap root composition according to claim 5, it is characterised in that：Described macleaya cordata-Huang
A kind of reed mentioned in ancient books composition as veterinary drug or animal pharmaceuticals feed addictive to use in the form of powder or pre-mixing agent.
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| CN107828866A (en) * | 2017-12-25 | 2018-03-23 | 云南牧道生物技术有限公司 | A kind of application for the macleaya cordata conditioning agent for improving weanling pig immune performance |
| CN108452014A (en) * | 2018-05-17 | 2018-08-28 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of pharmaceutical composition of purification kind salmonella gallinarum |
| CN108771703A (en) * | 2018-05-17 | 2018-11-09 | 中国农业科学院兰州畜牧与兽药研究所 | Application of the pharmaceutical composition in the drug for preparing prevention laying hen Salmonella infection disease |
| CN109198206A (en) * | 2017-07-07 | 2019-01-15 | 北京中联华康科技有限公司 | Radix scutellariae quaternary ammonium salt feed addictive |
| CN109362969A (en) * | 2018-11-07 | 2019-02-22 | 华中农业大学 | A kind of feed addictive and its application with raising except ammonia deodorizing effect |
| CN111513211A (en) * | 2020-06-01 | 2020-08-11 | 广州市天朝生物科技有限公司 | Water-soluble nutrient essential oil for livestock and poultry and aquaculture and preparation method and application thereof |
| CN112206256A (en) * | 2020-11-03 | 2021-01-12 | 河南黑马动物药业有限公司 | A pharmaceutical preparation for improving animal immunity and its preparation method |
| CN112655819A (en) * | 2020-11-30 | 2021-04-16 | 广西农业职业技术学院 | Anti-avian influenza feed additive for large-scale chicken raising and preparation method thereof |
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| CN109198206A (en) * | 2017-07-07 | 2019-01-15 | 北京中联华康科技有限公司 | Radix scutellariae quaternary ammonium salt feed addictive |
| CN107828866A (en) * | 2017-12-25 | 2018-03-23 | 云南牧道生物技术有限公司 | A kind of application for the macleaya cordata conditioning agent for improving weanling pig immune performance |
| CN108452014A (en) * | 2018-05-17 | 2018-08-28 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of pharmaceutical composition of purification kind salmonella gallinarum |
| CN108771703A (en) * | 2018-05-17 | 2018-11-09 | 中国农业科学院兰州畜牧与兽药研究所 | Application of the pharmaceutical composition in the drug for preparing prevention laying hen Salmonella infection disease |
| CN108452014B (en) * | 2018-05-17 | 2021-05-04 | 中国农业科学院兰州畜牧与兽药研究所 | Pharmaceutical composition for purifying seed of salmonella gallinarum |
| CN108771703B (en) * | 2018-05-17 | 2021-05-04 | 中国农业科学院兰州畜牧与兽药研究所 | Application of pharmaceutical composition in preparation of drugs for preventing and treating salmonellosis infection of laying hens |
| CN109362969A (en) * | 2018-11-07 | 2019-02-22 | 华中农业大学 | A kind of feed addictive and its application with raising except ammonia deodorizing effect |
| CN111513211A (en) * | 2020-06-01 | 2020-08-11 | 广州市天朝生物科技有限公司 | Water-soluble nutrient essential oil for livestock and poultry and aquaculture and preparation method and application thereof |
| CN112206256A (en) * | 2020-11-03 | 2021-01-12 | 河南黑马动物药业有限公司 | A pharmaceutical preparation for improving animal immunity and its preparation method |
| CN112655819A (en) * | 2020-11-30 | 2021-04-16 | 广西农业职业技术学院 | Anti-avian influenza feed additive for large-scale chicken raising and preparation method thereof |
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Application publication date: 20170531 |