CN108653498B - Spleen-tonifying and growth-promoting pharmaceutical composition and application thereof - Google Patents
Spleen-tonifying and growth-promoting pharmaceutical composition and application thereof Download PDFInfo
- Publication number
- CN108653498B CN108653498B CN201810593271.0A CN201810593271A CN108653498B CN 108653498 B CN108653498 B CN 108653498B CN 201810593271 A CN201810593271 A CN 201810593271A CN 108653498 B CN108653498 B CN 108653498B
- Authority
- CN
- China
- Prior art keywords
- parts
- growth
- spleen
- promoting
- test
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000008194 pharmaceutical composition Substances 0.000 title claims abstract description 19
- 239000003814 drug Substances 0.000 claims abstract description 183
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 117
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 74
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims abstract description 39
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims abstract description 39
- 239000001630 malic acid Substances 0.000 claims abstract description 39
- 235000011090 malic acid Nutrition 0.000 claims abstract description 39
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 37
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 37
- 235000015165 citric acid Nutrition 0.000 claims abstract description 37
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 37
- 239000011718 vitamin C Substances 0.000 claims abstract description 37
- 210000000582 semen Anatomy 0.000 claims abstract description 35
- 238000002360 preparation method Methods 0.000 claims abstract description 17
- 239000004480 active ingredient Substances 0.000 claims abstract description 10
- 244000144972 livestock Species 0.000 claims abstract description 10
- 241000208689 Eucommia ulmoides Species 0.000 claims abstract 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 42
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 37
- 238000002156 mixing Methods 0.000 claims description 34
- 238000000034 method Methods 0.000 claims description 18
- 239000000843 powder Substances 0.000 claims description 15
- 238000010298 pulverizing process Methods 0.000 claims description 15
- 238000005303 weighing Methods 0.000 claims description 13
- 239000003674 animal food additive Substances 0.000 claims description 10
- 239000002671 adjuvant Substances 0.000 claims description 9
- 239000008187 granular material Substances 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 4
- 238000007873 sieving Methods 0.000 claims description 3
- 239000002552 dosage form Substances 0.000 claims 1
- 210000000952 spleen Anatomy 0.000 abstract description 34
- 230000001737 promoting effect Effects 0.000 abstract description 31
- 230000000694 effects Effects 0.000 abstract description 29
- 239000002994 raw material Substances 0.000 abstract description 11
- 244000144977 poultry Species 0.000 abstract description 5
- 230000036528 appetite Effects 0.000 abstract description 3
- 235000019789 appetite Nutrition 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 3
- 208000024891 symptom Diseases 0.000 abstract description 3
- 230000007774 longterm Effects 0.000 abstract description 2
- 238000011084 recovery Methods 0.000 abstract description 2
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 230000007613 environmental effect Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 229940079593 drug Drugs 0.000 description 103
- 238000012360 testing method Methods 0.000 description 96
- 206010012735 Diarrhoea Diseases 0.000 description 61
- 241000282887 Suidae Species 0.000 description 34
- 239000000706 filtrate Substances 0.000 description 26
- 241000208688 Eucommia Species 0.000 description 25
- 238000005728 strengthening Methods 0.000 description 23
- 241001465754 Metazoa Species 0.000 description 19
- 240000007371 Cuscuta campestris Species 0.000 description 14
- 230000003405 preventing effect Effects 0.000 description 14
- 230000004584 weight gain Effects 0.000 description 14
- 235000019786 weight gain Nutrition 0.000 description 14
- 241000049624 Alisma plantago-aquatica subsp. orientale Species 0.000 description 12
- 230000007812 deficiency Effects 0.000 description 12
- 210000003734 kidney Anatomy 0.000 description 12
- 229920001353 Dextrin Polymers 0.000 description 11
- 239000004375 Dextrin Substances 0.000 description 11
- 229920002472 Starch Polymers 0.000 description 11
- 235000019425 dextrin Nutrition 0.000 description 11
- 238000002791 soaking Methods 0.000 description 11
- 235000019698 starch Nutrition 0.000 description 11
- 239000008107 starch Substances 0.000 description 11
- 238000005516 engineering process Methods 0.000 description 9
- 238000001914 filtration Methods 0.000 description 9
- 235000013305 food Nutrition 0.000 description 9
- 239000000463 material Substances 0.000 description 9
- 239000000825 pharmaceutical preparation Substances 0.000 description 9
- 230000035622 drinking Effects 0.000 description 8
- 244000237330 gutta percha tree Species 0.000 description 8
- 241000411851 herbal medicine Species 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- 241000699670 Mus sp. Species 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 6
- 201000010099 disease Diseases 0.000 description 6
- 238000001647 drug administration Methods 0.000 description 6
- 230000036541 health Effects 0.000 description 6
- 230000002265 prevention Effects 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 235000021050 feed intake Nutrition 0.000 description 5
- 238000000643 oven drying Methods 0.000 description 5
- 239000007779 soft material Substances 0.000 description 5
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 230000006378 damage Effects 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 210000004185 liver Anatomy 0.000 description 4
- 210000002784 stomach Anatomy 0.000 description 4
- 229940126680 traditional chinese medicines Drugs 0.000 description 4
- 239000004181 Flavomycin Substances 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- 210000001015 abdomen Anatomy 0.000 description 3
- 238000010171 animal model Methods 0.000 description 3
- 229940124350 antibacterial drug Drugs 0.000 description 3
- PERZMHJGZKHNGU-JGYWJTCASA-N bambermycin Chemical compound O([C@H]1[C@H](NC(C)=O)[C@@H](O)[C@@H]([C@H](O1)CO[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@@H]1O[C@@H]([C@H]([C@H](O)[C@H]1NC(C)=O)O[C@H]1[C@@H]([C@@H](O)[C@@H](O)[C@H](O1)C(=O)NC=1C(CCC=1O)=O)O)C)[C@H]1[C@@H](OP(O)(=O)OC[C@@H](OC\C=C(/C)CC\C=C\C(C)(C)CCC(=C)C\C=C(/C)CCC=C(C)C)C(O)=O)O[C@H](C(O)=O)[C@@](C)(O)[C@@H]1OC(N)=O PERZMHJGZKHNGU-JGYWJTCASA-N 0.000 description 3
- 230000006399 behavior Effects 0.000 description 3
- 230000037396 body weight Effects 0.000 description 3
- 230000013872 defecation Effects 0.000 description 3
- 235000019374 flavomycin Nutrition 0.000 description 3
- 230000037406 food intake Effects 0.000 description 3
- 235000012631 food intake Nutrition 0.000 description 3
- 230000037308 hair color Effects 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 238000000465 moulding Methods 0.000 description 3
- 229940076742 senna leaves Drugs 0.000 description 3
- 230000002195 synergetic effect Effects 0.000 description 3
- 239000002023 wood Substances 0.000 description 3
- 208000008035 Back Pain Diseases 0.000 description 2
- 235000006693 Cassia laevigata Nutrition 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- 206010024419 Libido decreased Diseases 0.000 description 2
- 241000522641 Senna Species 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 229940126678 chinese medicines Drugs 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000003255 drug test Methods 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 239000001530 fumaric acid Substances 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229940124513 senna glycoside Drugs 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 208000011580 syndromic disease Diseases 0.000 description 2
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 208000006820 Arthralgia Diseases 0.000 description 1
- 241000132012 Atractylodes Species 0.000 description 1
- 206010063659 Aversion Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 244000303040 Glycyrrhiza glabra Species 0.000 description 1
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 description 1
- 235000017443 Hedysarum boreale Nutrition 0.000 description 1
- 235000007858 Hedysarum occidentale Nutrition 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- 208000008930 Low Back Pain Diseases 0.000 description 1
- TURHTASYUMWZCC-UHFFFAOYSA-N Olaquindox [BAN:INN] Chemical compound C1=CC=C2N([O-])C(C)=C(C(=O)NCCO)[N+](=O)C2=C1 TURHTASYUMWZCC-UHFFFAOYSA-N 0.000 description 1
- 244000248825 Peltandra virginica Species 0.000 description 1
- 235000001188 Peltandra virginica Nutrition 0.000 description 1
- 206010035039 Piloerection Diseases 0.000 description 1
- 235000008599 Poria cocos Nutrition 0.000 description 1
- 208000003286 Protein-Energy Malnutrition Diseases 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 206010038084 Rectocele Diseases 0.000 description 1
- 206010057040 Temperature intolerance Diseases 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 208000022531 anorexia Diseases 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- XKNKHVGWJDPIRJ-UHFFFAOYSA-N arsanilic acid Chemical compound NC1=CC=C([As](O)(O)=O)C=C1 XKNKHVGWJDPIRJ-UHFFFAOYSA-N 0.000 description 1
- 229950002705 arsanilic acid Drugs 0.000 description 1
- 208000019804 backache Diseases 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 235000021316 daily nutritional intake Nutrition 0.000 description 1
- 206010061428 decreased appetite Diseases 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000001947 glycyrrhiza glabra rhizome/root Substances 0.000 description 1
- 230000036449 good health Effects 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 208000027905 limb weakness Diseases 0.000 description 1
- 231100000861 limb weakness Toxicity 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 206010026820 marasmus Diseases 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 229950010210 olaquindox Drugs 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000005371 pilomotor reflex Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- XMVJITFPVVRMHC-UHFFFAOYSA-N roxarsone Chemical compound OC1=CC=C([As](O)(O)=O)C=C1[N+]([O-])=O XMVJITFPVVRMHC-UHFFFAOYSA-N 0.000 description 1
- 229960003052 roxarsone Drugs 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 239000000273 veterinary drug Substances 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 230000004580 weight loss Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/46—Eucommiaceae (Eucommia family), e.g. hardy rubber tree
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/194—Carboxylic acids, e.g. valproic acid having two or more carboxyl groups, e.g. succinic, maleic or phthalic acid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/365—Lactones
- A61K31/375—Ascorbic acid, i.e. vitamin C; Salts thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/39—Convolvulaceae (Morning-glory family), e.g. bindweed
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/43—Cuscutaceae (Dodder family), e.g. Cuscuta epithymum or greater dodder
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/884—Alismataceae (Water-plantain family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/12—Antidiarrhoeals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Polymers & Plastics (AREA)
- Epidemiology (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Alternative & Traditional Medicine (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Immunology (AREA)
- Animal Husbandry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Nutrition Science (AREA)
- Physiology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a preparation method and application of a spleen-tonifying and growth-promoting pharmaceutical composition. The medicine contains active ingredients, wherein the active ingredients are prepared from eucommia ulmoides, semen cuscutae, rhizoma alismatis, citric acid, malic acid, vitamin C and the like. The spleen-tonifying and growth-promoting pharmaceutical composition has the effects of tonifying spleen, promoting appetite, enhancing body constitution, and promoting rapid growth of livestock and poultry, can be effectively used for treating symptoms such as slow growth and weak constitution of livestock and poultry, rehabilitation after recovery of livestock and poultry, and the like, and has the advantages of wide raw material source, safety, environmental protection, simple preparation process, low production cost, small dosage, long-term addition and the like.
Description
Technical Field
The invention relates to a preparation method and application of a spleen-tonifying and growth-promoting pharmaceutical composition, belonging to the field of veterinary medicines.
Background
With the development of intensive breeding industry, antibacterial drugs are added into the feed at low dosage, so that the disease can be prevented, the utilization rate of the feed can be improved, the growth of animals can be promoted, and the breeding benefit can be improved. However, long-term addition of the antibacterial growth promoter in low dosage can induce pathogenic bacteria to generate drug resistance, cause animal-derived food drug residues, further cause food safety problems and public health problems, and damage human health. In view of these problems, the european union and the united states have recently been banning the use of antibacterial agents as growth promoting additives in food animal feeds. The banning anti-call in China is increasing day by day, and 3 kinds of growth-promoting veterinary drugs such as olaquindox, arsanilic acid, roxarsone and the like are banned from food animals in 2019, 5 months and 1 days. Therefore, research and development of growth-promoting drugs which are substituted for antibiotics, safe and can be added for a long time are urgent needs of livestock intensive culture.
The diarrhea is an important factor influencing the growth of the livestock and poultry, can reduce the feed conversion rate, induce other diseases, cause death or growth and development obstruction due to untimely treatment or improper treatment method, delay the slaughtering time and increase the feeding cost. In recent years, a large amount of various antibacterial drugs are clinically and blindly used for treating diarrhea, the effect is not ideal, but the drug resistance of bacteria is increased, the elimination speed of the antibacterial drugs is accelerated, and the intestinal diarrhea diseases of livestock and poultry cause huge loss to the breeding industry and become important factors for restricting the development of the livestock industry.
According to the basic theory of Chinese veterinarian, the cause of diarrhea of early weaned piglets is caused by food stagnation and food retention impairment due to weakness of spleen and stomach of the piglets. When the spleen and stomach are affected by the invasion of damp pathogen, the food enters the stomach, the food is not transformed, the essence is not distributed, and the food is polluted, resulting in diarrhea. Therefore, the prevention and treatment of diarrhea should be mainly to resolve dampness, activate spleen, invigorate spleen and tonify deficiency. Therefore, the Chinese herbal medicine preparation for strengthening the spleen, tonifying qi, strengthening the body resistance and cultivating vigor can be developed, the appetite of the piglets can be enhanced, the immunity and the resistance to stress of the piglets can be improved, the physique of the piglets can be enhanced, the diarrhea harm can be reduced, and the growth can be promoted.
CN200710017503.X discloses a kidney invigorating and yang strengthening Chinese medicinal preparation, which uses 22 kinds of Chinese medicines, wherein semen Cuscutae, Eucommiae cortex, and Alismatis rhizoma have the effects of invigorating kidney and strengthening yang. CN201110023224.0 discloses a Chinese herbal medicine feed additive for increasing litter size of fox nests, which uses 18 traditional Chinese medicines, wherein dodder, eucommia bark and alisma orientale are mainly used for supplementing kidney essence so as to improve reproductive capacity of organisms. CN201410789490.8 the present invention discloses a traditional Chinese medicine for treating backup boar hyposexuality, which uses 17 traditional Chinese medicines, wherein dodder, eucommia bark and alisma orientale are mainly used for invigorating kidney and strengthening yang, so as to achieve the effects of treating backup boar hyposexuality and increasing sperm number. CN201410789025.4 the present invention discloses a boar feed, wherein 8 Chinese herbal medicines are added in the boar feed, wherein dodder, eucommia bark and alisma orientale are mainly used for tonifying kidney and replenishing vital essence to achieve the effect of improving the quality of boar sperm. CN201610917565.5 discloses a Chinese herbal medicine feed additive and a preparation method thereof, the Chinese herbal medicine feed additive uses 23 kinds of Chinese herbal medicines, wherein, dodder, eucommia bark and alisma orientale are mainly used for tonifying kidney and strengthening bones so as to achieve the effects of disease resistance and survival rate improvement. CN201711020990.5 discloses a Chinese herbal medicine feed additive and a preparation method thereof, the Chinese herbal medicine feed additive uses 14 Chinese medicines, wherein, dodder, eucommia bark and alisma orientale are mainly used for tonifying kidney and strengthening tendons so as to achieve the effects of resisting diseases and improving survival rate.
In conclusion, the development of drugs capable of promoting growth and preventing diarrhea is a solution to the problems in the livestock breeding industry and is an urgent need.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a safe, environment-friendly and effective spleen-tonifying and growth-promoting pharmaceutical composition.
The invention also aims to provide a preparation method of the spleen-tonifying and growth-promoting pharmaceutical composition.
The spleen-tonifying and growth-promoting pharmaceutical composition contains active ingredients, wherein the active ingredients are prepared from eucommia ulmoides, semen cuscutae, rhizoma alismatis, citric acid, malic acid and vitamin C.
The active ingredients are prepared from 40-80 parts of eucommia bark, 30-60 parts of semen cuscutae, 10-30 parts of rhizoma alismatis, 1-10 parts of citric acid, 1-10 parts of malic acid and 78-10 parts of vitamin C1.
Further, the active ingredients are prepared from 50-60 parts of eucommia ulmoides, 40-50 parts of semen cuscutae, 15-25 parts of rhizoma alismatis, 3-8 parts of citric acid, 3-8 parts of malic acid and 78-8 parts of vitamin C3.
Furthermore, the active ingredients are prepared from 55 parts of eucommia bark, 45 parts of semen cuscutae, 20 parts of rhizoma alismatis, 5 parts of citric acid, 5 parts of malic acid and 5 parts of vitamin C.
The invention also provides a preparation method of the spleen-invigorating and growth-promoting pharmaceutical composition, which comprises the following steps:
(1) weighing eucommia ulmoides, semen cuscutae and rhizoma alismatis according to the weight ratio to serve as a medicinal component 1;
(2) weighing citric acid, malic acid and vitamin C as the medicinal component 2 according to the weight ratio;
(3) pulverizing the weighed medicine component 1, sieving, or extracting the weighed medicine component with water or alcohol, concentrating, adding medicine component 2, mixing, and adding pharmaceutically acceptable adjuvants or auxiliary components.
The preparation form of the spleen-tonifying and growth-promoting pharmaceutical composition prepared by the preparation method can be as follows: premix, powder, extract powder, granule, and oral liquid.
The invention also provides application of the spleen-tonifying growth-promoting pharmaceutical composition in preparation of a livestock growth-promoting feed additive.
The spleen-strengthening and growth-promoting pharmaceutical composition provided by the invention is used as a feed additive alone, and can also be used in combination with other conventional additives, so that the invention also provides a feed additive containing the spleen-strengthening and growth-promoting pharmaceutical composition.
The eucommia ulmoides, the semen cuscutae and the rhizoma alismatis are used for strengthening spleen and promoting growth, and the three medicines have a synergistic effect when being used together with the citric acid, the malic acid and the vitamin C, so that the effects of promoting growth and preventing and treating diarrhea can be achieved remarkably. Eucommia bark, dodder seed and alisma orientale are used simultaneously in patents of CN200710017503.X, CN201110023224.0, CN201410789490.8, CN201410789025.4, CN201610917565.5, CN201711020990.5 and the like, and mainly play roles of tonifying kidney, nourishing kidney, replenishing vital essence and strengthening yang. The five-element theory of traditional Chinese medicine indicates that the liver and kidney are homologous, the kidney is water and the liver is wood, and the kidney water can nourish liver wood, but if the liver wood is too strong, spleen soil is restrained, and the spleen and the stomach are exteriorly-interiorly connected, so that the digestion and absorption functions are influenced. Therefore, compared with the prior art, the essence of the application of the three traditional Chinese medicines is different, and the three traditional Chinese medicines can be properly combined only through a great deal of research, so that a good growth promoting effect is exerted, otherwise, the effect is undesirable.
The medicinal components and the dosage thereof in the spleen-invigorating and growth-promoting medicinal composition are obtained according to dialectical treatment and a large number of repeated practices of Chinese veterinary prescriptions. Eucommia bark is used for treating lumbago and backache, tonifying middle-jiao, benefiting vital essence, strengthening muscles and bones and strengthening the mind; the eucommia bark has good growth promoting effect. Dodder mainly has the effects of curing wound, tonifying deficiency, tonifying qi and building up body; the south dodder seed is sweet in flavor and can help spleen and treat spleen deficiency. Rhizoma alismatis mainly treats wind-cold-dampness arthralgia, eliminates water, nourishes five internal organs, benefits strength and is fertile and healthy; it can nourish the spleen by purging dampness, so it can tonify qi and strengthen body. The medicine provided by the invention combines the eucommia bark, the dodder seed and the alisma orientale together from the angle of tonifying spleen, so that the medicine can invigorate the spleen to promote growth, has a stronger effect, and can be fed for a long time without causing adverse effects on animal organisms. The citric acid and the malic acid are organic acids, and the combination of the citric acid and the malic acid has a synergistic effect, so that the feed intake of cultured animals can be obviously improved, the feed conversion rate and the weight gain can be improved, pathogenic microorganisms can be effectively killed, and diarrhea can be prevented, thereby promoting the growth. The vitamin C can improve the immunity of the organism and improve the disease resistance of the cultured animals, thereby keeping the cultured animals in a good health state. The eucommia bark, the dodder seed and the alisma orientale are further combined with the citric acid, the malic acid and the vitamin C to show a better growth promoting effect, and after the composition is prepared into a common preparation in pharmacy, the composition can effectively prevent the diarrhea of weaned piglets, enhance the appetite of the piglets, improve the immunity and the resistance of the piglets to weaning stress, enhance the physique of the piglets, reduce the harm of the diarrhea of the piglets and promote the growth of the piglets.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
EXAMPLE 1 pharmaceutical preparation and use of the invention
(1) Weighing the following raw materials by weight:
80 parts of eucommia bark, 60 parts of semen cuscutae, 30 parts of rhizoma alismatis, 10 parts of citric acid, 10 parts of malic acid and 10 parts of vitamin C.
(2) Pulverizing Eucommiae cortex, semen Cuscutae and Alismatis rhizoma, soaking in water for 30min, decocting for 3 times, each for 1.5 hr, mixing filtrates, concentrating into soft extract, adding citric acid, malic acid and vitamin C, adding appropriate amount of starch and dextrin (or other adjuvants), mixing, making into soft material, granulating, oven drying, and making into granule (each 1g is equivalent to 1g of crude drug).
(3) The application method of the medicine comprises the following steps: strengthening the spleen, promoting growth and preventing diarrhea of weaned pigs; mixed feeding: 0.5-2g of the medicine is added into every 1kg of the feed of the pigs, and the pigs can be freely fed; the medicine is mixed for drinking, 0.25-1g of the medicine is added into 1L of water, and the medicine is freely drunk.
Example 2 pharmaceutical preparation and use of the invention
(1) Weighing the following raw materials by weight:
40 parts of eucommia, 30 parts of semen cuscutae, 10 parts of rhizoma alismatis, 1 part of citric acid, 1 part of malic acid and 1 part of vitamin C.
(2) Pulverizing Eucommiae cortex, semen Cuscutae and Alismatis rhizoma, soaking in water for 30min, decocting for 3 times, each for 1.5 hr, mixing filtrates, concentrating into soft extract, adding citric acid, malic acid and vitamin C, adding appropriate amount of starch and dextrin (or other adjuvants), mixing, making into soft material, granulating, oven drying, and making into granule (each 1g is equivalent to 1g of crude drug).
(3) The application method of the medicine comprises the following steps: strengthening the spleen, promoting growth and preventing diarrhea of weaned pigs; mixed feeding: 0.5-2g of the medicine is added into every 1kg of the feed of the pigs, and the pigs can be freely fed; the medicine is mixed for drinking, 0.25-1g of the medicine is added into 1L of water, and the medicine is freely drunk.
Example 3 pharmaceutical preparation and use of the invention
(1) Weighing the following raw materials by weight:
50 parts of eucommia bark, 40 parts of south dodder seed, 15 parts of oriental waterplantain rhizome, 3 parts of citric acid, 3 parts of malic acid and 3 parts of vitamin C.
(2) Pulverizing Eucommiae cortex, semen Cuscutae and Alismatis rhizoma, soaking in water for 30min, decocting for 3 times, each for 1.5 hr, mixing filtrates, concentrating into soft extract, adding citric acid, malic acid and vitamin C, adding appropriate amount of starch and dextrin (or other adjuvants), mixing, making into soft material, granulating, oven drying, and making into granule (each 1g is equivalent to 1g of crude drug).
(3) The application method of the medicine comprises the following steps: strengthening the spleen, promoting growth and preventing diarrhea of weaned pigs; mixed feeding: 0.5-2g of the medicine is added into every 1kg of the feed of the pigs, and the pigs can be freely fed; the medicine is mixed for drinking, 0.25-1g of the medicine is added into 1L of water, and the medicine is freely drunk.
Example 4 pharmaceutical preparation and use of the invention
(1) Weighing the following raw materials by weight:
60 parts of eucommia bark, 50 parts of dodder, 25 parts of alisma orientale, 8 parts of citric acid, 8 parts of malic acid and 8 parts of vitamin C.
(2) Pulverizing Eucommiae cortex, semen Cuscutae and Alismatis rhizoma, soaking in water for 30min, decocting for 3 times, each for 1.5 hr, mixing filtrates, concentrating into soft extract, adding citric acid, malic acid and vitamin C, adding appropriate amount of starch and dextrin (or other adjuvants), mixing, making into soft material, granulating, oven drying, and making into granule (each 1g is equivalent to 1g of crude drug).
(3) The application method of the medicine comprises the following steps: strengthening the spleen, promoting growth and preventing diarrhea of weaned pigs; mixed feeding: 0.5-2g of the medicine is added into every 1kg of the feed of the pigs, and the pigs can be freely fed; the medicine is mixed for drinking, 0.25-1g of the medicine is added into 1L of water, and the medicine is freely drunk.
Example 5 pharmaceutical preparation and use of the invention
(1) Weighing the following raw materials by weight:
55 parts of eucommia bark, 45 parts of semen cuscutae, 20 parts of rhizoma alismatis, 5 parts of citric acid, 5 parts of malic acid and 5 parts of vitamin C.
(2) Pulverizing Eucommiae cortex, semen Cuscutae and Alismatis rhizoma, soaking in water for 30min, decocting for 3 times, each for 1.5 hr, mixing filtrates, concentrating into soft extract, adding citric acid, malic acid and vitamin C, adding appropriate amount of starch and dextrin (or other adjuvants), mixing, making into soft material, granulating, oven drying, and making into granule (each 1g is equivalent to 1g of crude drug).
(3) The application method of the medicine comprises the following steps: strengthening the spleen, promoting growth and preventing diarrhea of weaned pigs; mixed feeding: 0.5-2g of the medicine is added into every 1kg of the feed of the pigs, and the pigs can be freely fed; the medicine is mixed for drinking, 0.25-1g of the medicine is added into 1L of water, and the medicine is freely drunk.
Example 6 pharmaceutical preparation and use of the invention
(1) Weighing the following raw materials by weight:
80 parts of eucommia bark, 30 parts of semen cuscutae, 25 parts of rhizoma alismatis, 10 parts of citric acid, 1 part of malic acid and 10 parts of vitamin C.
(2) Pulverizing Eucommiae cortex, semen Cuscutae and Alismatis rhizoma, soaking in water for 30min, decocting for 3 times, each for 1.5 hr, mixing filtrates, concentrating to obtain a certain volume (equivalent to 1g/ml of crude drug), adding ethanol until ethanol content in filtrate is 70%, filtering, recovering ethanol from filtrate, adding citric acid, malic acid and vitamin C, mixing, adding appropriate amount of starch and dextrin (or other adjuvants), mixing, and making into extract powder (each 1g is equivalent to 1g of crude drug)
(3) The application method of the medicine comprises the following steps: strengthening the spleen, promoting growth and preventing diarrhea of weaned pigs; mixed feeding: 0.5-2g of the medicine is added into every 1kg of the feed of the pigs, and the pigs can be freely fed; the medicine is mixed for drinking, 0.25-1g of the medicine is added into 1L of water, and the medicine is freely drunk.
Example 7 pharmaceutical preparation and use of the invention
(1) Weighing the following raw materials by weight:
60 parts of eucommia bark, 40 parts of south dodder seed, 15 parts of oriental waterplantain rhizome, 5 parts of citric acid, 2 parts of malic acid and 4 parts of vitamin C.
(2) Mixing the raw materials weighed in the step (1), micronizing, sieving with 200 mesh sieve, mixing well, and making into powder.
(3) The application method of the medicine comprises the following steps: strengthening the spleen, promoting growth and preventing diarrhea of weaned pigs; mixed feeding: the medicine of the invention is added into every 1kg of feed of pigs by 0.5-2g, and the pigs can be freely fed.
Example 8 pharmaceutical preparation and use of the invention
(1) Weighing the following raw materials by weight:
60 parts of eucommia bark, 40 parts of south dodder seed, 10 parts of oriental waterplantain rhizome, 6 parts of citric acid, 2 parts of malic acid and 10 parts of vitamin C.
(2) Pulverizing Eucommiae cortex, semen Cuscutae and Alismatis rhizoma, soaking in water for 30min, decocting for 3 times, each for 1.5 hr, mixing filtrates, concentrating to obtain a certain volume (equivalent to 1g/ml of crude drug), adding ethanol until ethanol content in filtrate is 70%, filtering, recovering ethanol from filtrate, adding citric acid, malic acid and vitamin C, mixing, adding appropriate amount of starch and dextrin (or other adjuvants), mixing, and making into extract powder (each 1g is equivalent to 1g of crude drug)
(3) The application method of the medicine comprises the following steps: strengthening the spleen, promoting growth and preventing diarrhea of weaned pigs; mixed feeding: the medicine of the invention is added into every 1kg of feed of pigs by 0.5-2g, and the pigs can be freely fed.
Example 9 pharmaceutical preparation and use of the invention
(1) Weighing the following raw materials by weight:
80 parts of eucommia bark, 30 parts of semen cuscutae, 20 parts of rhizoma alismatis, 6 parts of citric acid, 2 parts of malic acid and 10 parts of vitamin C.
(2) Pulverizing Eucommiae cortex, semen Cuscutae and Alismatis rhizoma, adding water, soaking for 30min, decocting for 3 times, each time for 1.5 hr, mixing filtrates, concentrating to a certain volume (equivalent to 1g/ml of crude drug), adding ethanol until the ethanol content in the filtrate is 70%, filtering, concentrating to an amount of 0.5g per 1ml of crude drug, adding citric acid, malic acid and vitamin C, filtering, bottling, and sterilizing to obtain oral liquid. (0.5 g per 1ml of crude drug)
(3) The application method of the medicine comprises the following steps: strengthening the spleen, promoting growth and preventing diarrhea of weaned pigs; mixing and drinking: 0.5-2ml of the medicine is added into 1L of water of the pig, and the pig can drink freely.
In order to show the effects of the medicine of the invention on strengthening spleen, promoting growth and preventing diarrhea, clinical curative effect observation tests are carried out on the medicine of the invention, and the beneficial effects of the medicine of the invention are further illustrated by test examples.
Test example 1
In order to verify the curative effect of the medicine, the influence of the medicine on a senna model mouse is investigated in an experimental animal house of the research center of animal health care technology of the great northern agricultural group, and a traditional Chinese medicine preparation for treating the animal spleen deficiency syndrome and promoting the growth is screened by a senna type spleen deficiency modeling method, wherein the experimental result is as follows:
1 materials and methods
1.1 animal model replication
And (3) taking a mouse with the weight of 24-26g, and carrying out gastric lavage by using the water decoction of the senna leaves of 0.3ml/20g for 8 days continuously, wherein the mice are fasted every other day, and the mice have the symptoms of withered and lusterless hair, evacuation, loose stool, weight, physical strength, food consumption reduction, cold intolerance and the like, namely successful model building.
The spleen deficiency animal model standard is as follows: firstly, patients with serious diarrhea even rectocele; ② eating less and anorexia; ③ marasmus and weight loss; metal depression, limb weakness, hair curling, low body temperature and fatigue.
1.2 preparation of test drugs
Extracting the senna leaves: pulverizing folium sennae, decocting in water 16 times for 3 times (each for 1.5 hr), filtering, and concentrating the filtrate to 1 g/ml.
Preparation of test drugs: test drugs were prepared according to pharmaceutical examples 1-5 of the present invention, respectively.
Sijunzi san, made by oneself, mainly made into dangshen, white atractylodes rhizome, licorice root, tuckahoe.
1.3 test grouping and administration
Clean-grade Kunming mice 80 were divided into 8 groups of 10 mice each. Experiment 1 group administered with the drug of the present invention prepared in example 1, with drinking water, 1g of the present invention was added per 1L of water; experiment 2 group administered with the drug prepared in inventive drug example 2, 1g of the drug of the present invention was added per 1L of water; experiment 3 groups administered with the drug prepared in inventive drug example 3, with water, 1g of the drug of the present invention was added per 1L of water; experiment 4 groups administered with the drug prepared in inventive drug example 4, with water, 1g of the drug of the present invention was added per 1L of water; experiment 5 groups of the drugs prepared in inventive drug example 5 were administered with water, 1g of the inventive drug was added per 1L of water; the test 6 groups adopt four-monarch-seed powder, mixed feeding is carried out, and 2g of the four-monarch-seed powder is added into each kilogram of feed; test 7 group is model group; test 8 groups were healthy controls. Continuously performing intragastric administration for 8 days for other administration groups and spleen deficiency model groups except the healthy control group, and drinking distilled water for the healthy control group during molding; after each group had spleen deficiency symptoms, the administration group drunk the test liquid respectively, and continued for 7 days, and the healthy control group and the spleen deficiency and spleen deficiency model group drunk distilled water. The feeding conditions of all groups are consistent.
1.4 examination of body weight and food intake
The feed intake of each group of test animals was recorded daily and each test group was weighed before molding, after molding and at the end of the test.
1.5 statistical methods
Values in each group are expressed as mean. + -. standard deviation
The results show that the comparison among the groups adopts variance analysis, the data of each group are pairwise compared, the significant difference is statistically significant when P is less than 0.05, and all data are statistically processed by adopting PASW Statistics 18.0 edition analysis software.
2 test results and analysis
The mice have loose stool and lose weight next day after the gastric lavage and the water decoction of the senna leaves, and then have spleen deficiency states of crinkling, aversion to cold, piloerection, slight temperature drop, reduced activity and the like. The condition is recovered after the treatment of the medicine and the four-monarch-drug powder, which is close to a normal group, but the recovery of a model group is not obvious. The death and weight gain during the mouse trial are shown in table 1.
TABLE 1 test results of the effect of the drug of the present invention on weight gain in spleen deficient mice
Note: the weight gain of the test groups 1-5, the Sijunzi san group and the model group is 0.000, 0.003, 0.000, 0.002, 0.229, 0.000 and 0.000 respectively compared with the healthy group; compared with the model group, the p values of the test group 1-5 and the Sijunzi san group are respectively 0.000, 0.000 and 0.038; compared with the four-monarch powder group, the p values of the test groups 1-5 are 0.016, 0.001, 0.010, 0.001 and 0.000.
The test results show that compared with the model group, each test group of the medicament can remarkably improve the weight gain of mice (P <0.01), and the effect of the medicament in the aspect of weight gain is remarkably superior to that of the control medicament Sijunzi san (P < 0.05). The drug test 5 group has the best weight gain effect on mice with spleen deficiency syndrome, and is higher than other drug test groups and control drug Sijunzi san groups.
Test example 2
In order to verify the curative effect of the medicine, the research center of animal health care technology of northern Yangtze farm in Hebei province verifies the growth promoting effect test of the medicine on the weaned pig, and the test results are as follows:
1 materials and methods
1.1 test animals
The Duda Bai ternary hybrid is used for weaning piglets for 28 days.
1.2 feed and drugs
Feed: is a conservation material produced by certain feed science and technology limited liability company.
Test drugs: prepared according to the invention as pharmaceutical example 6.
Control drug 1: pulverizing 80 parts of eucommia bark, 30 parts of semen cuscutae and 25 parts of rhizoma alismatis, adding water to soak the medicinal materials for 30min, decocting for 3 times, 1.5 hours each time, combining the filtrates, concentrating to a certain volume (equivalent to 1g/ml of the original medicine), adding ethanol until the ethanol content in the filtrate is 70%, filtering, recovering ethanol from the filtrate, adding a proper amount of starch and dextrin, fully mixing uniformly, and preparing into extract powder (each 1g of the extract powder is equivalent to 1g of the original medicine).
Control drug 2: taking 10 parts of citric acid and 1 part of malic acid, and mixing uniformly for later use as a control medicament 2.
Control drug 3: and (3) vitamin C.
Control drug 4: a contrast medicament 4 is prepared according to the prescription of 80 parts of eucommia bark, 30 parts of semen cuscutae, 25 parts of rhizoma alismatis, 10 parts of citric acid and 1 part of malic acid. Pulverizing Eucommiae cortex, semen Cuscutae and Alismatis rhizoma, adding water, soaking for 30min, decocting for 3 times, each time for 1.5 hr, mixing filtrates, concentrating to a certain volume (equivalent to 1g/ml of crude drug), adding ethanol until the ethanol content in the filtrate is 70%, filtering, recovering ethanol from the filtrate, adding citric acid and malic acid, mixing, adding appropriate amount of starch and dextrin, mixing completely, and making into extract powder (each 1g is equivalent to 1g of crude drug).
Control drug 5: preparing a control medicament 5 according to the prescription of 80 parts of eucommia bark, 30 parts of semen cuscutae, 25 parts of rhizoma alismatis and 10 parts of vitamin C. Pulverizing Eucommiae cortex, semen Cuscutae and Alismatis rhizoma, soaking in water for 30min, decocting for 3 times, each for 1.5 hr, mixing filtrates, concentrating to obtain a certain volume (equivalent to 1g/ml of crude drug), adding ethanol until the ethanol content in the filtrate is 70%, filtering, recovering ethanol from the filtrate, adding vitamin C, mixing, adding appropriate amount of starch and dextrin, mixing, and making into extract powder (each 1g is equivalent to 1g of crude drug).
Control drug 6: taking 10 parts of citric acid, 1 part of malic acid and 10 parts of vitamin C, and mixing uniformly to obtain a control drug 6.
Control drug 7: preparing a control medicament 5 according to the prescription of 80 parts of eucommia bark, 30 parts of semen cuscutae, 25 parts of rhizoma alismatis, 11 parts of fumaric acid and 10 parts of vitamin C. Pulverizing Eucommiae cortex, semen Cuscutae and Alismatis rhizoma, adding water, soaking for 30min, decocting for 3 times, each time for 1.5 hr, mixing filtrates, concentrating to a certain volume (equivalent to 1g/ml of crude drug), adding ethanol until the ethanol content in the filtrate is 70%, filtering, recovering ethanol from the filtrate, adding fumaric acid and vitamin C, mixing, adding appropriate amount of starch and dextrin, mixing completely, and making into extract powder (each 1g is equivalent to 1g of crude drug).
1.3 test grouping
540 weaned piglets are randomly divided into 9 groups, the test 1 group is a test drug group, 1g of the drug prepared in the 6 examples of the drug is added into 1kg of feed, mixed feeding is carried out, and the drug is continuously fed for 28 days; in the group 1 of the control drugs, 1g of the control drug 1 is added into 1kg of feed, mixed feeding is carried out, and the drug administration is carried out continuously for 28 days; in the group 2 of the control medicaments, 1g of the control medicament 2 is added into each 1kg of feed, mixed feeding is carried out, and the continuous feeding is carried out for 28 days; in the 3 groups of the control drugs, 1g of the control drug 3 is added into each 1kg of feed, mixed feeding is carried out, and the drug administration is carried out continuously for 28 days; in the 4 groups of the control medicaments, 1g of the control medicament 4 is added into each 1kg of feed, mixed feeding is carried out, and the continuous administration is carried out for 28 days; in the 5 groups of the control drugs, 1g of the control drug 5 is added into each 1kg of feed, mixed feeding is carried out, and the drug administration is carried out continuously for 28 days; in the 6 groups of the control drugs, 1g of the control drug 6 is added into each 1kg of feed, mixed feeding is carried out, and the drug administration is carried out continuously for 28 days; adding 1g of the control medicament 7 into 1kg of feed in 7 groups of the control medicaments, and carrying out mixed feeding administration for 28 days continuously; the feed of the blank control group is not added with any medicine.
1.4 survey index
The abdomen was weighed at 28 days old and at 7 am at 56 days old, and piglets were observed and recorded for feeding, body condition, hair color, behavior, defecation, diarrhea, etc.
2 test results and analysis
The weight gain of piglets is shown in table 2.
TABLE 2 piglet weight gain test results
The test results show that, compared with the blank control group, the test drugs and the control drugs 1 to 7 have good growth promoting effects on piglets, wherein the test drug prepared according to the drug example 6 of the invention has the best growth promoting effect. The effect of the drug prepared according to the drug embodiment 6 of the present invention on piglet growth promotion is better than that of the control drug 1 (3 traditional Chinese medicine compositions such as eucommia ulmoides, semen cuscutae and rhizoma alismatis are used alone) or the control drug 2 (citric acid and malic acid composition used alone) or the control drug 3 (vitamin C used alone), and is also better than that of the control drug 4 (eucommia ulmoides, semen cuscutae, rhizoma alismatis, citric acid and malic acid composition) or the control drug 5 (eucommia ulmoides, semen cuscutae, rhizoma alismatis and vitamin C composition) and is also better than that of the control drug 6 (citric acid, malic acid and vitamin C composition), which indicates that the combined use of the eucommia ulmoides, semen cuscutae and rhizoma alismatis, the citric acid, malic acid and vitamin C has a synergistic. The effect of the drug prepared according to drug example 6 of the present invention on promoting growth of piglets is better than that of the control drug 7, which indicates that citric acid and malic acid used in the present invention are the best choice and should not be replaced by other acidulants.
Test example 3
In order to further verify the growth promotion effect of the medicament on the weaned piglets, a comparative test is carried out in a certain test pig farm in Huanghai and hai in Hubei by the research center of animal health technology of agricultural groups in North China, and the test results are as follows:
1 materials and methods
1.1 test animals
And (5) growing the Du-growing triply-hybridized 28-day weaned piglets.
1.2 feed and drugs
Feed: a conservation material produced by certain feed science and technology limited liability company;
test drugs: prepared according to the invention as pharmaceutical example 7.
Control drugs: 4% flavomycin premix
1.3 test grouping
300 weaned piglets for 28 days are randomly divided into 3 groups, the test 1 group is a test drug group, 1k g prepared according to the invention in the drug embodiment 7 is added into each 1 ton of feed, and the mixed feeding and the drug administration are carried out for 28 days; the test 2 group is a control drug group, 125g of 4% flavomycin premix is added into each 1 ton of feed, and mixed feeding and drug administration are carried out for 28 days continuously; the test 3 group was a blank control group, and no drug was added to the feed.
1.4 survey index
The abdomen was weighed at 28 days old and at 7 am at 56 days old, and piglets were observed and recorded for feeding, body condition, hair color, behavior, defecation, diarrhea, etc.
2 test results and analysis
The weight gain of piglets is shown in table 3.
TABLE 3 piglet weight gain test results
The results of the piglet diarrhea prevention test are shown in table 4.
TABLE 4 piglet diarrhea prevention test results
| Group of | Death number (head) | Diarrhea digital (head) | Diarrhea Rate (%) |
| Test 1 group | 0 | 0 | 0 |
| Test 2 groups | 1 | 2 | 2 |
| Test 3 groups | 3 | 15 | 15 |
The test results show that compared with a blank control group, the test drug and the control drug have good growth promoting effect and piglet diarrhea prevention effect, and the growth promoting effect and the diarrhea prevention effect of the drug are obviously superior to those of the antibacterial growth promoting control drug flavomycin. During the test period, the pigs were healthy well, indicating that the drug of the invention is safe and can be added for a long time.
Test example 4
To further verify the growth promoting effect of the medicament on the growing-finishing pigs, the research center of animal health care technology of the northern agricultural group carries out a comparative test in a test pig farm of Zhu Dong Zhu city, and the test results are as follows:
1 materials and methods
1.1 test animals
Growing fattening pigs of 70 days old by three-way hybridization.
1.2 feed and drugs
Feed: the fertilizer is a growth fertilizer produced by certain feed science and technology limited liability company;
test drugs: prepared according to the invention as pharmaceutical example 8.
1.3 test grouping
Randomly dividing 200 growing-finishing pigs of 70 days old into 2 groups, wherein the test 1 group is a test drug group, 0.5kg of the drug prepared in the drug example 8 is added into each 1 ton of feed, and the drug is mixed and administered for 28 days; the test 2 group was a blank control group, and no drug was added to the feed.
1.4 survey index
The abdomen of each group was weighed at 70 days old and 98 days old at 7 am, and the condition of ingestion, body condition, hair color, behavior, defecation, diarrhea, etc. of the pigs were observed and recorded.
2 test results and analysis
The weight gain of growing-finishing pigs is shown in Table 5.
TABLE 5 growth promoting test results for growing-finishing pigs
The test result shows that compared with a blank control group, the test medicament has good growth promoting effect on growing-finishing pigs. During the test period, the pigs were healthy well, indicating that the drug of the invention is safe and can be added for a long time.
Test example 5
In order to verify the curative effect of the medicine, the research center of animal health care technology of northern great agriculture group verifies the prevention effect of the medicine on the diarrhea of weaned piglets in certain test pig farm of Tianjin Diwu, and the results are as follows:
1 materials and methods
1.1 test animals
The three-element miscellaneous piglet, Duda Bai, has 20 litters, 9-l 2 litters and 209 litters. The difference of the date of birth of the experimental piglets is not more than 2 days, the weight and the number of births are similar, the sex proportion is consistent, and the sow has no disease history.
1.2 feed and drugs
Feed: granular feed for porket produced by certain feed science and technology Limited liability company;
test drugs: prepared according to the invention as pharmaceutical example 9.
1.3 test grouping
The 20 litter piglets were randomly divided into a test group (10 litter 103) and a control group (10 litter 106). Both the test group and the control group were weaned at 28 days of age. The test group was administered from the beginning of 21 days old to 3 weeks (49. sup. th.) after weaning with 1kg of the drug prepared in accordance with pharmaceutical example 9 of the present invention per ton of the feed, and the control group was administered without any drug.
1.4 survey index
The test group and the control group are respectively weighed at the time and space of 21 days old, 28 days old, 35 days old, 42 days old and 49 days old 7 am, and the feed input amount of each group per day and the feed residual amounts of 28 days old, 35 days old, 42 days old and 49 days old are recorded from 21 days old. The dung excretion status of the piglets is observed and recorded one by one at 7 hours and 20 hours each day from the age of 28 days.
1.5 data statistics
And (4) counting the feed intake before and after weaning of the piglets, the average weight before weaning, the weight gain of the piglets, the feed-weight ratio, the number of dead piglets after weaning, the number of diarrhea piglets, the diarrhea rate and the diarrhea frequency. All data were statistically processed using PASW Statistics version 18.0 analysis software and subjected to significance analysis.
Diarrhea rate is the number of diarrhea heads/total number of diarrhea heads
Diarrhea frequency ═ diarrhea first/(number of heads per group × number of days post-weaning test)
2 results of the test
2.1 diarrhea preventing Effect
As can be seen from table 6: the test group died 1, the control group died 5, and the difference between the two groups was significant (P < 0.05); the diarrhea of the test group is 34, the diarrhea of the control group is 59, and the difference between the two groups is significant (P < 0.05); the diarrhea rate of the test group is 33.0 percent, the diarrhea rate of the control group is 55.7 percent, and the difference between the two groups is obvious (P is less than 0.05); the diarrhea frequency of the test group is 1.57, the diarrhea frequency of the control group is 2.65, and the difference between the two groups is significant (P < 0.05).
TABLE 6 preventive effect of the inventive drug on diarrhea of weaned piglets
| Group of | Death number (head) | Diarrhea digital (head) | Diarrhea Rate (%) | Diarrhea frequency (%) |
| Test group | 1 | 34 | 33.0 | 1.57 |
| Control group | 5 | 59 | 55.7 | 2.65 |
2.2 feed intake
As can be seen from table 7: the difference of the feed intake of the 21-28-day-old test group and the control group is not obvious; after 28 days of age, the food intake of the test group is obviously higher than that of the control group, and the difference is obvious (P < 0.05).
TABLE 7 daily food intake (kg)
| Group of | 21-28 days old | 28-35 days old | 35-42 days old | Age of 42-49 days |
| Test group | 0.52 | 0.66 | 0.86 | 1.03 |
| Control group | 0.53 | 0.62 | 0.79 | 0.88 |
2.3 weight gain Effect
As can be seen from table 8: the average body weight difference between the test group and the control group is not significant at the age of 21-28 days; compared with a control group, the average weight of piglets in the test group after 28-mesh age is obviously improved.
TABLE 8 average body weight (kg) at each day's age
| Group of | Age of 21 days | Age of 28 days | Age of 35 days | Age of 42 days | Age 49 days old |
| Test group | 5.35 | 7.67 | 10.91 | 13.79 | 18.31 |
| Control group | 5.33 | 7.42 | 10.58 | 12.65 | 15.53 |
2.4 feed conversion ratio
As can be seen from table 9: the material weight ratio of the test group after weaning is obviously lower than that of the control group, which indicates that the feed conversion rate of the test group is higher than that of the control group.
TABLE 9 feed weight ratio for each age of day
| Group of | 21-28 days old | 28-35 days old | 35-42 days old | Age of 42-49 days |
| Test group | 1.77 | 1.61 | 1.99 | 1.58 |
| Control group | 1.65 | 1.77 | 2.73 | 1.83 |
Discussion of 3
Indexes such as the number of heads of diarrhea, diarrhea rate, diarrhea frequency and the like are adopted to investigate the diarrhea degree, the difference between a test group and a control group is obvious, and the result can objectively reflect the good effect of the medicine on preventing the diarrhea of the weaned piglets.
The piglet has larger stress and is easy to cause diarrhea and induce other diseases due to the drastic change of the feeding environment and the feeding conditions within 2 weeks after the piglet is weaned, so the daily gain and the feed conversion rate are reduced. In this growth stage, piglets are susceptible to diseases, and should be kept under increased management to minimize stress.
The medicine is added into the piglet feed 1 week before weaning, so that the piglet constitution can be improved, the resistance to weaning stress is enhanced, the harm of piglet diarrhea is reduced, and the piglet growth is promoted.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the technical principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (6)
1. The spleen-invigorating and growth-promoting pharmaceutical composition contains active ingredients, wherein the active ingredients are prepared from 50-60 parts of eucommia ulmoides, 40-50 parts of semen cuscutae, 15-25 parts of rhizoma alismatis, 3-8 parts of citric acid, 3-8 parts of malic acid and 3-8 parts of vitamin C.
2. The spleen-invigorating and growth-promoting pharmaceutical composition according to claim 1, wherein the active ingredients are prepared from 55 parts of eucommia ulmoides, 45 parts of semen cuscutae, 20 parts of rhizoma alismatis, 5 parts of citric acid, 5 parts of malic acid and 5 parts of vitamin C.
3. A method for preparing the spleen-invigorating, growth-promoting pharmaceutical composition according to claim 1 or 2, comprising the steps of: 1) weighing eucommia ulmoides, semen cuscutae and rhizoma alismatis according to the weight ratio to serve as a medicinal component 1; 2) weighing citric acid, malic acid and vitamin C as the medicinal component 2 according to the weight ratio; 3) pulverizing the weighed medicine component 1, sieving, or extracting the weighed medicine component 1 with water or alcohol, concentrating, adding the medicine component 2, mixing, and adding pharmaceutically acceptable adjuvants or auxiliary components.
4. The method for preparing the spleen-invigorating and growth-promoting pharmaceutical composition according to claim 3, wherein the prepared dosage form is: powder, granule or oral liquid.
5. The use of the spleen-invigorating, growth-promoting pharmaceutical composition of claim 1 or 2 in the preparation of a livestock growth-promoting feed additive.
6. A feed additive comprising the spleen-invigorating, growth-promoting pharmaceutical composition according to claim 1 or 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810593271.0A CN108653498B (en) | 2018-06-11 | 2018-06-11 | Spleen-tonifying and growth-promoting pharmaceutical composition and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810593271.0A CN108653498B (en) | 2018-06-11 | 2018-06-11 | Spleen-tonifying and growth-promoting pharmaceutical composition and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108653498A CN108653498A (en) | 2018-10-16 |
| CN108653498B true CN108653498B (en) | 2021-08-13 |
Family
ID=63775539
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810593271.0A Active CN108653498B (en) | 2018-06-11 | 2018-06-11 | Spleen-tonifying and growth-promoting pharmaceutical composition and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108653498B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785528A (en) * | 2010-02-01 | 2010-07-28 | 西安沃森生物科技有限公司 | Eucommia ulmoides feed additive and preparation method and application thereof |
| CN106615846A (en) * | 2017-01-10 | 2017-05-10 | 湘西芙蓉资源农业科技有限公司 | Traditional Chinese medicine health-care feed for middle and big pigs and preparation method of traditional Chinese medicine health-care feed |
-
2018
- 2018-06-11 CN CN201810593271.0A patent/CN108653498B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785528A (en) * | 2010-02-01 | 2010-07-28 | 西安沃森生物科技有限公司 | Eucommia ulmoides feed additive and preparation method and application thereof |
| CN106615846A (en) * | 2017-01-10 | 2017-05-10 | 湘西芙蓉资源农业科技有限公司 | Traditional Chinese medicine health-care feed for middle and big pigs and preparation method of traditional Chinese medicine health-care feed |
Non-Patent Citations (3)
| Title |
|---|
| 复合酸化剂对断奶仔猪生产性能和肠道微生物区系的影响;汪海峰等;《中国畜牧杂志》;20110610(第11期);第49-53页 * |
| 杜仲提取物对猪生长性能及血液指标的影响;王建辉等;《饲料研究》;20070210(第02期);第1页右栏最后1部分及第2页左栏第1部分、第2页右栏最后1部分、第3页右栏第2段 * |
| 添加Vc可提高猪增重;《北京农业》;19950505(第05期);第26页第3列第3部分 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108653498A (en) | 2018-10-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101167918B (en) | Traditional Chinese medicinal preparation for increasing immunity of pig or poultry | |
| CN102356823B (en) | Chinese herbal medicine additive for reducing stress reaction of a weaned pig and pig feed thereof | |
| CN101698024B (en) | Traditional Chinese medicine for treating respiratory diseases and promoting growth for swine and preparation method thereof | |
| CN101869224B (en) | Feed additive for improving lactation performance of lactating sows and preparation method thereof | |
| CN101703171B (en) | Natural vegetal anti-weaning stress feed additive and application thereof | |
| CN105230568A (en) | Breeding method of high-immunity and high-quality live pigs | |
| CN1935182A (en) | Chinese herbal medicine compound immunopotentiator for animal | |
| CN106819482A (en) | Improve antibiotic-free feed of milking sow production performance and preparation method thereof | |
| CN103156061A (en) | Compound microbial active additive | |
| CN103006916A (en) | Pure traditional Chinese medicine recipe for curing various animal diarrhoeal diseases and preparation method of pure traditional Chinese medicine recipe | |
| CN105124164A (en) | High-efficient pig feed containing Chinese herbal medicines and making method of high-efficient pig feed | |
| CN111248347A (en) | Compound feed additive for livestock and poultry to remove mildew and detoxify and preparation method thereof | |
| CN101791339B (en) | Traditional Chinese veterinary medicine capable of tonifying spleen and stimulating appetite | |
| CN103735965A (en) | Traditional Chinese medicinal prescription for preventing and treating trichomonad of pigeon, as well as preparation method and application thereof | |
| CN106578484A (en) | Chinese herbal medicine feed supplement for sows in suckling period | |
| CN108653498B (en) | Spleen-tonifying and growth-promoting pharmaceutical composition and application thereof | |
| CN101757136A (en) | Traditional Chinese medicine composite for treating viral swine high fever | |
| CN106818635A (en) | A kind of method for breeding for improving calf survival rate | |
| CN105748873A (en) | Veterinary traditional Chinese medicine particle for treating livestock and poultry respiratory tract infection | |
| CN114191510B (en) | Chinese herbal compound preparation for preventing and treating diarrhea of piglets and preparation method thereof | |
| CN104666524A (en) | Stomach-invigorating traditional Chinese medicine for livestock | |
| CN103006889B (en) | Traditional Chinese medicine preparation for promoting lactation of female livestock and preparation method and using method thereof | |
| CN104415100A (en) | Compound traditional Chinese medicine powder for treating cow mastitis | |
| CN114887007B (en) | Traditional Chinese medicine composition for preventing and treating bighead carp bleeding disease, feed, and preparation method and application thereof | |
| CN103385368A (en) | Chinese medicine preparation for preventing and treating postpartum hypogalactia of sow and production method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220720 Address after: 101407 Beijing city Huairou District Yanqi Economic Development Zone No. 10 North Street Patentee after: Beijing Dabeinong Animal Health Care Technology Co.,Ltd. Patentee after: Shaoshan Dabeinong Animal Medicine Co.,Ltd. Address before: 101407 Beijing city Huairou District Yanqi Economic Development Zone No. 10 North Street Patentee before: Beijing Dabeinong Animal Health Care Technology Co.,Ltd. Patentee before: Shaoshan Dabeinong Animal Medicine Co.,Ltd. Patentee before: BEIJING DABEINONG TECHNOLOGY GROUP Co.,Ltd. |