CN104940375A - Production method for lactic acid bacteria fermented poplar flower oral liquid - Google Patents
Production method for lactic acid bacteria fermented poplar flower oral liquid Download PDFInfo
- Publication number
- CN104940375A CN104940375A CN201510291659.1A CN201510291659A CN104940375A CN 104940375 A CN104940375 A CN 104940375A CN 201510291659 A CN201510291659 A CN 201510291659A CN 104940375 A CN104940375 A CN 104940375A
- Authority
- CN
- China
- Prior art keywords
- lactic acid
- acid bacteria
- poplar
- flower
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Relating to microorganism fermented Chinese medicinal poultry drugs, the invention provides a production method for a lactic acid bacteria fermented poplar flower oral liquid. The method includes: taking 80-100mL of poplar flower water, 3-5g of soybean meal, 3-5g of brown sugar and 1.5-2.5g of peptone; coordinating the raw materials, conducting high-pressure sterilization, inoculating lactic acid bacteria accounting for 1% of the weight of the raw materials, and carrying out anaerobic fermentation at 37DEG C for 48h, thus obtaining the oral liquid. According to the invention, lactic acid bacteria are employed to ferment poplar flower water, at the end of fermentation, the fermentation broth contains two effective ingredients, i.e. lactic acid bacteria and fermentation type poplar flower extract. During application, the product can give play to the intestinal flora regulating, animal immunity enhancing and pathogenic microorganism inhibiting effects of lactic acid bacteria, and also can bring the poplar flower's efficacy of tonifying spleen and nourishing stomach, resolving dampness and stopping dysentery, relieving diarrhea with astringents, clearing away heat and toxic materials into play. Also, after fermentation in vitro, poplar flowers are easier to absorb, thereby better achieving the treatment purpose. The drug involved in the invention has the advantages of treatment of both symptoms and root causes, rapid efficacy, pure green and no residue, and excellent curative effect. 167 cases of clinical tests show that the total effective rate reaches 97.0%.
Description
Technical field
The present invention relates to fowl medicine, is fermentable Chinese medicine class fowl medicine further, particularly treats the Chinese medicine microecological preparation of the caused diarrhoea of chicken pathogen mixed infection and enteritis.
Technical background
Lactobacillus is a kind of microbe additive of work, often add to use in feedstuff, to growth of animal, body health, there is important function, intestinal environment facilitating digestion can be improved, suppress pathogen, play health invigorating, control the pathogenetic effect of disease, be verified clinically.
The inflorescence of Salicaceous Plants Canada of flower of poplar system willow, be rich in the compositions such as vitamin, alkaloid, organic acid, phenols and alkaloid, there is effect of strengthening the spleen and nourishing the stomach, removing dampness dysentery relieving, relieving diarrhea with astringents, heat-clearing and toxic substances removing, be commonly used for the good medicine for the treatment of pig, sheep, fowl dysentery.
Fermentation technique is produced with Chinese herbal medicine and is combined by the present invention, develops a kind of poplar flower oral liquid of lactic acid bacteria fermentation, may be used for treating the diarrhoea caused by the mixed infection of chicken pathogen.
Summary of the invention
The object of the present invention is to provide a kind of production method of lactic acid bacteria fermentation poplar flower oral liquid, it can utilize lactic acid bacteria fermentation poplar flower extract, make a kind of poplar flower oral liquid of lactic acid bacteria fermentation, be used for the treatment of the caused diarrhoea of chicken pathogen mixed infection and enteritis; And there is the effect improving chicken immunity, preventing chicken diseases.
The object of the invention is to be realized by following scheme:
The production method of lactic acid bacteria fermentation poplar flower oral liquid, is characterized in that: get flower of poplar water 80-100mL, bean cake 3-5 gram, brown sugar 3-5 gram, peptone 1.5-2.5 gram; After being worked good by raw material, autoclaving, the above all raw material weights of inoculation (flower of poplar water, bean cake, brown sugar, peptone weight sum, lower same) 1% lactic acid bacteria culturers, 37 DEG C of anaerobic fermentation 48h both obtain.
Object of the present invention can realize further by following scheme:
Each component the best is: flower of poplar water 90mL, bean cake 4 grams, 4 grams, brown sugar, peptone 2 grams; After being worked good by raw material, autoclaving, the lactic acid bacteria culturers of the above all raw material weights 1% of inoculation, 37 DEG C of anaerobic fermentation 48h both obtained.
Described flower of poplar water refers to: flower of poplar is placed in extraction element, adds 5 times to the water of flower of poplar weight, boils and extracts medicinal liquid in 30 minutes; Then the water adding 3 times, 3 times more respectively according to upper method carries out the extraction of second and third time, mix three extracting solution concentrated.Concentrated solution specification is that 1mL is equivalent to crude drug in whole 1 gram.
Described autoclaving refers to 121 DEG C of autoclaving 30min.
Described peptone refers to animal proteinum peptone, especially beef peptone.
Described lactic acid bacteria culturers specification is containing Lactobacillus plantarum 1 × 10 in every milliliter of strain
8individual, streptococcus faecalis 2 × 10
8individual, enterococcus faecalis 1 × 10
8individual.
Raw of the present invention: peptone, English name: peptone is organic compound.Peptone is that meat, casein or gelatin is sour or after protease hydrolysis, dry outward appearance is flaxen powder, has the special breath of meat perfume (or spice).Protein also can form peptone after acid, alkali or proteases for decomposing.Bean cake, brown sugar, peptone (beef peptone) all adopt food grade products.Strain can directly be bought, and also can make by oneself, and during self-control, in picking glycerol cryopreservation tube, lactobacillus solid spawn is a little, and access liquid MRS culture medium, 37 DEG C of anaerobic fermentation 24h both obtained.
Configuration mechanism of the present invention: the inflorescence of Salicaceous Plants Canada of flower of poplar system willow, be rich in the compositions such as vitamin, alkaloid, organic acid, phenols and alkaloid, there is effect of strengthening the spleen and nourishing the stomach, removing dampness dysentery relieving, relieving diarrhea with astringents, heat-clearing and toxic substances removing, be commonly used for the good medicine for the treatment of pig, sheep, fowl dysentery.Lactobacillus is having under moisture and anaerobic condition and can grow in poplar flower extract, add brown sugar and peptone can improve lactic acid bacterium number, make lactobacillus can play Fermentation to poplar flower extract better, be conducive to the release of effective ingredient in medicine; Meanwhile, lactobacillus can produce new active substance in metabolic process, improves the action effect of medicine.
Beneficial effect of the present invention: with lactic acid bacteria fermentation flower of poplar water, containing two kinds of effective ingredient, i.e. lactobacillus and fermented type poplar flower extract in the after fermentation liquid that fermented.This product can play lactobacillus regulating intestinal canal flora, improving animal body immunity, suppressing pathogenic microorganism effect when applying, effect of flower of poplar strengthening the spleen and nourishing the stomach, removing dampness dysentery relieving, relieving diarrhea with astringents, heat-clearing and toxic substances removing can be played again, simultaneously, flower of poplar water more easily absorbs after In Vitro Fermentation, can better reach therapeutic purposes.Medicine of the present invention, rapid, the pure green noresidue for the treatment of both the principal and secondary aspects of a disease, drug effect, curative effect are better.Show with 167 routine clinical trials, total effective rate reaches 97.0%.Under the best circumstances, tunning lactobacillus bacterium number can reach 2.0 × 10
9cfu/g; Thus scalable chicken intestinal flora, suppresses pathogenic microorganism, improves chicken immunity of organisms, thus effectively reduces the generation of chicken disease particularly intestinal tract disease, and through 296 example tests, average attack rate reduces by 28.7%.
According to correlation test data, effect of the present invention is described below.
testing data:
(1) to cure the disease aspect:
1, bacterial diarrhea is cured mainly.
2, case statistics: treat in 167 examples, broiler 144 example, laying hen 23 example.The course of disease is the shortest 1 day, the longest 3 days.
3, Therapeutic Method: every for this product 250mL is watered 1000 jin and freely drinks to sick chicken, also can calculate the once concentrated dispensing of total amount of drinking water every day.Each course for the treatment of 3-5 days, each course for the treatment of interval 3-5 days, can again with the next course for the treatment of.General 1 the course for the treatment of clinical symptoms just can substantially disappear.Afterwards, re-use 1 course for the treatment of, consolidate curative effect, in case recurrence.
4, contraindication: Deficiency and coldness of spleen and stomach case is cautious use of.
5, efficacy assessment standard:
Cure: symptom of diarrhea disappear, the chicken group mental status and feed intake normal.Take a turn for the better: diarrhea rate reduces, and the mental status and feed intake reach normal substantially.Invalid: not reach improvement target person.
6, therapeutic effect:
The shortest 2 days, the longest 5 days, 119 examples are cured in medication, account for 71.3%; Take a turn for the better 43 examples, accounts for 25.7%; Invalid 5 examples, account for 3.0%; Total effective rate 97.0%.
7, model case introduction:
Case 1: broiler field, Wang Zhu township, Puyang high and new technology industrial development zone, Henan Province, morbidity broiler 8 age in days, sickness rate 60%, symptom is that feed intake declines, spirit is not good enough, draws green loose stool, uses antibiotic 2 days, invalid.Sooner or later concentrate dispensing 2 times by this product, have positive effect after 1 day, after 3 days, clinical symptoms all disappears.
Case 2: broiler field, Anxiang, Anyang in Henan province county two, morbidity broiler 13 age in days, sickness rate 70%, symptom is lassitude, punishment in advance, yellow green watery stool, and use this product sooner or later to concentrate dispensing 2 times, have positive effect after 2 days, after 4 days, clinical symptoms all disappears.
(2) diseases prevention aspect:
Every for this product 250mL is watered 2000 jin freely to drink to sick chicken, during chickling, every 1 week feeding once, and during young chicken, every 2 weeks feedings once.Within 2013-2014 years, carry out contrast test in broiler field in town through the ages in broiler field, normal society mouth town, Hua County, Henan Province, Hua County, Henan Province, select 4 batches of totally 296 chickling feedings, by above-mentioned requirements feeding, compared with 160 chickling matched groups of not this medicine of feeding, disease incidence does not reduce 25.1-32.4% not etc., and average attack rate reduces by 28.7%.
In order to make those skilled in the art person understand the present invention program better, and above-mentioned purpose of the present invention, characteristic sum a little can be become apparent more, below in conjunction with embodiment, the present invention is further detailed explanation.
Detailed description of the invention
Embodiment 1: get flower of poplar water 90mL, bean cake 4 grams, 4 grams, brown sugar, peptone 2 grams, after raw material is worked good, autoclaving, inoculation above all raw material weights (flower of poplar water, bean cake, brown sugar, peptone weight sum, the lactic acid bacteria culturers of 1% down together), anaerobic fermentation 48h both obtained, and fermentation liquid lactobacillus bacterium number is 2.0 × 10
9cfu/mL.
Embodiment 2: get flower of poplar water 90mL, bean cake 3 grams, 5 grams, brown sugar, peptone 2 grams, after being worked good by raw material, autoclaving, the lactic acid bacteria culturers of the above all raw material weights 1% of inoculation, anaerobic fermentation 48h both obtained, and fermentation liquid lactobacillus bacterium number is 1.8 × 10
9cfu/mL.
Embodiment 3: get flower of poplar water 90mL, bean cake 5 grams, 3 grams, brown sugar, peptone 2 grams, after being worked good by raw material, autoclaving, the lactic acid bacteria culturers of the above all raw material weights 1% of inoculation, anaerobic fermentation 48h both obtained, and fermentation liquid lactobacillus bacterium number is 1.7 × 10
9cfu/mL.
Embodiment 4: get flower of poplar water 80mL, bean cake 3 grams, 3 grams, brown sugar, peptone 1.5 grams; After being worked good by raw material, autoclaving, above all raw material weight 1% lactic acid bacteria culturerss of inoculation, it is 1.8 × 10 that 37 DEG C of anaerobic fermentation 48h both obtain fermentation liquid lactobacillus bacterium number
9cfu/mL.
Embodiment 5: get flower of poplar water 100mL, bean cake 5 grams, 5 grams, brown sugar, peptone 2.5 grams; After being worked good by raw material, autoclaving, above all raw material weight 1% lactic acid bacteria culturerss of inoculation, it is 1.9 × 10 that 37 DEG C of anaerobic fermentation 48h both obtain fermentation liquid lactobacillus bacterium number
9cfu/mL.
Embodiment 6: get flower of poplar water 89mL, bean cake 3.9 grams, 3.8 grams, brown sugar, peptone 1.8 grams; After being worked good by raw material, autoclaving, above all raw material weight 1% lactic acid bacteria culturerss of inoculation, it is 1.7 × 10 that 37 DEG C of anaerobic fermentation 48h both obtain fermentation liquid lactobacillus bacterium number
9cfu/mL.
In above embodiment:
Described flower of poplar water refers to: flower of poplar is placed in extraction element, adds 5 times to the water of flower of poplar weight, boils and extracts medicinal liquid in 30 minutes; Then the water adding 3 times, 3 times more respectively according to upper method carries out the extraction of second and third time, mix three extracting solution concentrated.Concentrated solution specification is that 1mL is equivalent to crude drug in whole 1 gram.
Described peptone can be the one of beef peptone, Carnis caprae seu ovis peptone.
Described autoclaving refers to 121 DEG C of autoclaving 30min.
Described lactic acid bacteria culturers specification is containing Lactobacillus plantarum 1 × 10 in every milliliter of strain
8individual, streptococcus faecalis 2 × 10
8individual, enterococcus faecalis 1 × 10
8individual.
The above; be only the specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, is anyly familiar with those skilled in the art in the technical scope that the present invention discloses; the change that can expect easily or replacement, all should be encompassed within protection scope of the present invention.
Claims (7)
1. the production method of lactic acid bacteria fermentation poplar flower oral liquid, is characterized in that: get flower of poplar water 80-100mL, bean cake 3-5 gram, brown sugar 3-5 gram, peptone 1.5-2.5 gram; After being worked good by raw material, autoclaving, above all raw material weight 1% lactic acid bacteria culturerss of inoculation, 37 DEG C of anaerobic fermentation 48h both obtained.
2. the production method of lactic acid bacteria fermentation poplar flower oral liquid according to claim 1, is characterized in that: flower of poplar water 90mL, bean cake 4 grams, 4 grams, brown sugar, peptone 2 grams; After being worked good by raw material, 121 DEG C of autoclaving 30min, the lactic acid bacteria culturers of the above all raw material weights 1% of inoculation, 37 DEG C of anaerobic fermentation 48h both obtained.
3. the production method of lactic acid bacteria fermentation poplar flower oral liquid according to claim 1 and 2, is characterized in that: described flower of poplar water refers to: flower of poplar is placed in extraction element, adds 5 times to the water of flower of poplar weight, boils and extracts medicinal liquid in 30 minutes; Then the water adding 3 times, 3 times more respectively according to upper method carries out the extraction of second and third time, mix three extracting solution concentrated.
4. the production method of lactic acid bacteria fermentation poplar flower oral liquid according to claim 1 and 2, is characterized in that: described peptone refers to animal proteinum peptone.
5. the production method of lactic acid bacteria fermentation poplar flower oral liquid according to claim 1 and 2, is characterized in that: described peptone refers to beef peptone.
6. the production method of lactic acid bacteria fermentation poplar flower oral liquid according to claim 1 and 2, is characterized in that: described autoclaving refers to 121 DEG C of autoclaving 30min.
7. the production method of lactic acid bacteria fermentation poplar flower oral liquid according to claim 1 and 2, is characterized in that: described lactic acid bacteria culturers specification is containing Lactobacillus plantarum 1 × 10 in every milliliter of strain
8individual, streptococcus faecalis 2 × 10
8individual, enterococcus faecalis 1 × 10
8individual.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510291659.1A CN104940375A (en) | 2015-06-01 | 2015-06-01 | Production method for lactic acid bacteria fermented poplar flower oral liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510291659.1A CN104940375A (en) | 2015-06-01 | 2015-06-01 | Production method for lactic acid bacteria fermented poplar flower oral liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104940375A true CN104940375A (en) | 2015-09-30 |
Family
ID=54155803
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510291659.1A Pending CN104940375A (en) | 2015-06-01 | 2015-06-01 | Production method for lactic acid bacteria fermented poplar flower oral liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104940375A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107334021A (en) * | 2017-07-21 | 2017-11-10 | 东莞波顿香料有限公司 | Betelnutpalm male flower drink and preparation method thereof |
| CN114246898A (en) * | 2021-12-24 | 2022-03-29 | 河南后羿实业集团有限公司 | Preparation method of fermented traditional Chinese medicine preparation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101664106A (en) * | 2008-09-02 | 2010-03-10 | 天津生机集团有限公司 | Method for producing livestock/poultry feed additives by fermenting poplar flowers |
| CN102614288A (en) * | 2011-12-31 | 2012-08-01 | 郑州后羿制药有限公司 | Chinese herbal microecological preparation for preventing and treating chicken Colibacillosis and preparation method thereof |
-
2015
- 2015-06-01 CN CN201510291659.1A patent/CN104940375A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101664106A (en) * | 2008-09-02 | 2010-03-10 | 天津生机集团有限公司 | Method for producing livestock/poultry feed additives by fermenting poplar flowers |
| CN102614288A (en) * | 2011-12-31 | 2012-08-01 | 郑州后羿制药有限公司 | Chinese herbal microecological preparation for preventing and treating chicken Colibacillosis and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 中国兽药典委员会编: "《中华人民共和国兽药典•二部》", 30 April 2006 * |
| 张兆旺: "《中药药剂学》", 31 January 2003 * |
| 曾志平等: "《有机农业》", 31 January 2010 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107334021A (en) * | 2017-07-21 | 2017-11-10 | 东莞波顿香料有限公司 | Betelnutpalm male flower drink and preparation method thereof |
| CN107334021B (en) * | 2017-07-21 | 2020-07-17 | 东莞波顿香料有限公司 | Areca flower beverage and preparation method thereof |
| CN114246898A (en) * | 2021-12-24 | 2022-03-29 | 河南后羿实业集团有限公司 | Preparation method of fermented traditional Chinese medicine preparation |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102614288B (en) | Chinese herbal microecological preparation for preventing and treating chicken Colibacillosis and preparation method thereof | |
| CN110787247B (en) | Traditional Chinese medicine microecological preparation for preventing and treating livestock and poultry diarrhea and preparation method thereof | |
| CN103141702B (en) | Chinese herb extract feed additive capable of effectively preventing and treating porcine epizootic diarrhea | |
| CN102366021A (en) | Manufacturing method of poultry feed additive containing traditional Chinese medicinal activity probiotics | |
| CN105287790B (en) | A kind of fevervine extract and its application | |
| CN101926428B (en) | Probiotic gingko pollen freeze-dried powder and preparation method thereof | |
| CN110946907A (en) | Traditional Chinese medicine composition for young poultry, liquid fermented traditional Chinese medicine preparation and preparation method thereof | |
| CN104208240A (en) | Traditional Chinese herbal microecological preparation for preventing and treating piglet bacterial dysentery and preparation method thereof | |
| CN105767543A (en) | Stomach-strengthening and digestion-promoting traditional Chinese medicine micro-ecological preparation for piglet and preparation method thereof | |
| CN107384676A (en) | Probiotics fermented beverage and preparation method thereof | |
| CN101695529A (en) | Compound Chinese herbal medicinal preparation for preventing and controlling bacterial enteritis, black gill and red skin disease of freshwater fish | |
| CN106309506B (en) | Probiotics composition with menstruation regulating function and application thereof | |
| CN107232463A (en) | Positive gas-liquid drink of probiotics and preparation method thereof | |
| CN103039700B (en) | Feed additive for veterinary drug solid fermentation and preparation method of feed additive | |
| CN102764308A (en) | Drug for preventing bacterial diseases of fish and preparation method thereof | |
| CN107118997B (en) | A kind of feeding clostridium butyricum, the probiotics for promoting growth of animals or poultry and preparation method thereof | |
| CN107961260A (en) | Treatment, the medicament for preventing birds coliform diarrhea disease and preparation method thereof | |
| CN104940375A (en) | Production method for lactic acid bacteria fermented poplar flower oral liquid | |
| CN101904499B (en) | Method for preparing probiotics and ginkgo pollen oral solution | |
| CN103404709B (en) | Feed additive produced from waste liquid and dregs in pharmaceutical factory and preparation method of feed additive | |
| CN103191207B (en) | Perpetration for treating chicken intestinal tract escherichia coli diarrhea and preparation method thereof | |
| CN109221697A (en) | Weaned piglets probiotic feed and preparation method thereof | |
| CN106343148A (en) | Chinese herbal medicine green feed additive | |
| CN108850613A (en) | Fattening pannage and preparation method thereof | |
| KR101107764B1 (en) | The SJP-microbe which has a function of Anti-harmfulness microbe |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150930 |
|
| WD01 | Invention patent application deemed withdrawn after publication |