CN103191207B - Perpetration for treating chicken intestinal tract escherichia coli diarrhea and preparation method thereof - Google Patents
Perpetration for treating chicken intestinal tract escherichia coli diarrhea and preparation method thereof Download PDFInfo
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- CN103191207B CN103191207B CN201310108341.6A CN201310108341A CN103191207B CN 103191207 B CN103191207 B CN 103191207B CN 201310108341 A CN201310108341 A CN 201310108341A CN 103191207 B CN103191207 B CN 103191207B
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Abstract
The invention relates to the prevention and the treatment of chicken intestinal tract escherichia coli diarrhea, in particular to a synbiotics Chinese herbal composition for preventing and treating the chicken intestinal tract escherichia coli diarrhea, and discloses a traditional Chinese medicine composition for treating the chicken intestinal tract escherichia coli diarrhea. The traditional Chinese medicine composition consists of dianthus superbus, the root of Chinese pulsatilla, liquorice and dandelion; the synbiotics composition of the traditional Chinese medicine composition is proportioned by the traditional Chinese medicine composition and the synbiotics according to the weight ratio of (55-90):(5-10); within two weeks after the chickens go out of the shells, the chicken are fed by the preparation and the feed, so that the preparation can play a part in prevention; the chicken intestinal tract escherichia coli diarrhea can be cured by increasing the dose after the disease comes on; after the traditional Chinese medicines are jointly used, the composition is prominent in the treatment effect for the chicken intestinal tract escherichia coli diarrhea, so that the appetite can be improved; and after the synbiotics preparation is added, the traditional Chinese medicine composition has synergy function.
Description
Technical field
The present invention relates to the control of chickling intestinal coliform diarrhea, especially a kind of symphysis unit Chinese medicine compound of preventing and treating chickling intestinal coliform diarrhea disease.
Background technology
Carnis Gallus domesticus is the leading indicator of the vegetable basket project improving national life level, is also the key factor affecting price fluctuation.China's Carnis Gallus domesticus imbalance of supply and demand in 2010, causes the price rise of meat valency, causes the concern of country to aquaculture.
Poultry husbandry development key is chickling, and once had the saying of " supporting good chickling most to receive ", chickling is healthy and strong, and the broiler rate of animals delivered to the slaughter-house is high.Chickling is born and latter two week is called the chickling phase, now the most easily coliform diarrhea occurs.Suffer from the chickling slight of stature of coliform diarrhea, be unwilling to take food, have loose bowels at first, be infectious, intestinal mucosa has catarrhal inflammation, mesenteric lymph node mild swelling, the sick chicken intraperitoneal of minority has a small amount of glue peptone sample material, bladder mucosa hyporrhea, and treating chickling not in time can be dead.Chickling intestinal coliform diarrhea disease " is relied mainly on prevention, is prevented overweighting controlling ", but there is no good prevention and therapy medicine at present.
Summary of the invention
An object of the present invention is to provide with a kind of Chinese medicine composition, and it has effect of control chickling intestinal coliform diarrhea disease.
For achieving the above object, technical scheme of the present invention is:
Be used for the treatment of the Chinese medicine composition of chickling intestinal coliform diarrhea, be made up of the dry medical material of following weight portion: Herba Dianthi 35-50 part, Radix Pulsatillae 3-8 part, Radix Glycyrrhizae 5-15 part, Herba Taraxaci 8-25 part.
Further, described Chinese medicine composition, is made up of the dry medical material of following weight portion: Herba Dianthi 45 parts, the Radix Pulsatillae 6 parts, 13 parts, Radix Glycyrrhizae, Herba Taraxaci 22 parts.
Two of object of the present invention is to provide with a kind of symphysis unit's compositions and preparation thereof and the feedstuff containing said preparation, and they add symphysis unit element on the basis of described Chinese medicine composition, collaborative control chickling intestinal coliform diarrhea disease.
For achieving the above object, technical scheme of the present invention is:
Formed by described Chinese medicine composition and the first weight ratio proportioning according to 55-90: 5-10 of symphysis; Described symphysis unit be by bifidobacterium preparations and oligosaccharide according to 3-5: 5-10 weight ratio proportioning form, containing the number of active bifid bacteria in described every gram of symphysis unit is 200-450 hundred million, and described oligosaccharide is any one or more in fruit oligose, oligomeric xylose, dextrinosan and soybean oligo saccharide.
Further, described symphysis unit compositions, is formed by described Chinese medicine composition and the first weight ratio proportioning according to 80: 7 of symphysis; Described symphysis unit be by bifidobacterium preparations and oligosaccharide according to 5: 8 weight ratio proportioning form, be 40,000,000,000 containing the number of active bifid bacteria in described every gram of symphysis unit, described oligosaccharide is soybean oligo saccharide
The preparation of described symphysis unit compositions.
The feedstuff of the preparation containing described symphysis unit compositions.
Further, the preparation that mass fraction is the described symphysis unit compositions of 8-15% is contained in described feedstuff.
Three of object of the present invention is the preparation method of the preparation providing symphysis unit compositions, and the method is simple to operate, process stabilizing, is applicable to suitability for industrialized production.
For achieving the above object, technical scheme of the present invention is:
The preparation method of described preparation, specifically comprises the following steps: being inoculated by the 4-6% of bacillus bifidus by culture volume, is 6.0-8.0 at pH value, and 37 DEG C of constant temperature quiescent culture 24-72 hour, obtain bifidobacterium preparations; Medium of Bifidobacterium calculates composed of the following components by weight: peptone 0.1-1.0 part, Carnis Bovis seu Bubali cream 0.1-0.5 part, cysteine 0.1-0.2 part, sodium chloride 0.1-0.5 part, glucose 1-1.5 part, hepar siccatum 0.1-0.2 part, aqueous medium 90-99 part, sterilizing; The bifidobacterium preparations of formula ratio and the oligosaccharide of formula ratio are fully mixed, obtains described symphysis unit; By the decocting for Chinese herbal medicine liquid mixing that described symphysis unit and the described Chinese medicine composition of formula ratio obtain, obtain the preparation of symphysis unit compositions, in the preparation of described symphysis unit compositions, the concentration of bacillus bifidus is more than or equal to 1 × 10
7individual/mL.
Further, in the preparation method described in 6, described bifidobacterium preparations specifically comprises the following steps:
A expands kind of a daughter bacteria
Get strain inoculation on inclined-plane and enter to be equipped with Medium of Bifidobacterium in vitro, 37 DEG C of constant temperature quiescent culture 24 hours, obtain first order seed bacterium, stored refrigerated;
The preparation of B secondary seed bacterium
By the ratio of first order seed bacterium in the 4-6% of Medium of Bifidobacterium volume, be inoculated into sterilizing and cooling Medium of Bifidobacterium in, 37 DEG C of constant temperature quiescent culture 24h, obtain secondary seed bacterium; Stored refrigerated; Poured into by Medium of Bifidobacterium in microorganism fermentation tank, sterilizing also cools;
C fermentation culture
By step B gained secondary seed bacterium in sterilizing and the ratio inoculation of the 6-8% of the Medium of Bifidobacterium volume of cooling, stir, 37 DEG C of constant temperature quiescent culture 48-72h, obtain culture fluid;
D centrifuge dehydration
In the centrifugal 20min of 3000r/min after step C gained culture fluid is cooled, remove supernatant, get bacterium mud, add and be equivalent to bacterium mud weight 1-2 filler mix homogeneously doubly, dry under being placed in 40-50 DEG C of condition, obtain bifidobacterium preparations.
Further, in described preparation method: Medium of Bifidobacterium formula: peptone 0.6 part, Carnis Bovis seu Bubali cream 0.3 part, cysteine 0.15 part, 0.5 part, sodium chloride, glucose 1.2 parts, hepar siccatum 0.2 part, aqueous medium 95 parts.
Beneficial effect of the present invention is: the Chinese medicine composition being used for the treatment of chickling intestinal coliform diarrhea of the present invention, is made up of Herba Dianthi, the Radix Pulsatillae, Radix Glycyrrhizae, Herba Taraxaci, adopts water-boiling method acquisition medicinal liquid to add symphysis unit again and forms.Go out in latter two week of shell chickling, with this medicine and feedstuff mixing feeding chickling, can preventive effect be played; Strengthen dose after morbidity and can treat recovery from illness.Wherein, the sliding profit of Herba Dianthi, can fall YIN-fire, except five types of stranguria, and sharp blood vessels, the medicine of a cold nature of holding concurrently.Radix Pulsatillae bitter in the mouth, cold in nature; Return stomach, large intestine channel, there is heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery, effect of removing dampness and killing parasites.The effects such as Radix Glycyrrhizae has heat-clearing and toxic substances removing, gastral cavity abdomen.Herba Taraxaci has heat-clearing and toxic substances removing, effect of dispersing swelling and dissipating binds.Several Chinese medicine conjunctive use, have significant curative effect curative effect to treatment chickling intestinal coliform diarrhea, and can improve appetite.After adding symphysis unit preparation, there is synergistic function.
Detailed description of the invention
Embodiment 1 is used for the treatment of the preparation of the preparation of chickling intestinal coliform diarrhea
One, taking oligosaccharide is that each 10Kg of fruit oligose, oligomeric xylose, dextrinosan and soybean oligo saccharide is for subsequent use;
Two, the preparation of bifidobacterium preparations:
Strain is the bacillus bifidus (Bifidobacterium) that lyophilizing is preserved; Medium of Bifidobacterium calculates composed of the following components by weight: peptone 0.6 part, Carnis Bovis seu Bubali cream 0.3 part, cysteine 0.15 part, 0.5 part, sodium chloride, glucose 1.2 parts, hepar siccatum 0.2 part, aqueous medium 95 parts, pH6.8,115 DEG C of high pressure steam sterilization 30min;
1. expand kind of a daughter bacteria, get strain on inclined-plane (as an inoculating loop) and inoculate into Medium of Bifidobacterium being housed in vitro, 37 DEG C of constant temperature quiescent culture 24h, obtain first order seed bacterium, take out Refrigerator store, stand-by;
2. the preparation of secondary seed bacterium: by first order seed bacterium in Medium of Bifidobacterium volume 5% ratio, be inoculated in bacillus bifidus bacterium culture medium, 37 DEG C of constant temperature quiescent culture 24h, obtain secondary seed bacterium;
The preparation of the Medium of Bifidobacterium 3. produced: take each composition by the component ratio of Medium of Bifidobacterium and be dissolved in water, pour in microorganism fermentation tank, in 115 DEG C of steam sterilizations 30 minutes;
4. inoculate: Medium of Bifidobacterium to be produced is chilled to about 37 DEG C, under flame controls, add secondary seed bacterium, inoculate in the ratio of the 6-8% of Medium of Bifidobacterium volume, stir;
5. cultivate: close all breather valve, 37 DEG C of constant temperature culture 60h;
6. centrifuge dehydration: take out culture fluid in biological fermentation tank, refrigerator is kept in, centrifugal 20 minutes in 3000 revs/min after culture fluid cooling, removes supernatant, get bacterium mud and add 2 times of bran powder mix homogeneously, load disk, be placed in 45 DEG C of air dry ovens and dry, carry out bacillus bifidus qualification by GB4789-2012 method, and calculate total viable count, pack, obtain bifidobacterium preparations, in cool place, dry place keeps in Dark Place for subsequent use.
Three preparations being used for the treatment of the preparation of chickling intestinal coliform diarrhea
1 Traditional Chinese medicine composition formula being used for the treatment of chickling intestinal coliform diarrhea
Be used for the treatment of the Chinese medicine composition of chickling intestinal coliform diarrhea, be called for short Chinese medicine composition 1: be made up of the dry medical material of following weight portion: Herba Dianthi 35 parts, the Radix Pulsatillae 3 parts, 15 parts, Radix Glycyrrhizae, Herba Taraxaci 25 parts.
Be used for the treatment of the Chinese medicine composition of chickling intestinal coliform diarrhea, be called for short Chinese medicine composition 2: be made up of the dry medical material of following weight portion: Herba Dianthi 50 parts, the Radix Pulsatillae 8 parts, 5 parts, Radix Glycyrrhizae, Herba Taraxaci 8 parts.
Be used for the treatment of the Chinese medicine composition of chickling intestinal coliform diarrhea, be called for short Chinese medicine composition 3: be made up of the dry medical material of following weight portion: Herba Dianthi 45 parts, the Radix Pulsatillae 6 parts, 13 parts, Radix Glycyrrhizae, Herba Taraxaci 22 parts.
The formula of 2 symphysis unit compositionss containing described Chinese medicine composition
Symphysis unit compositions containing above-mentioned Chinese medicine composition 1, is called for short symphysis unit compositions 1: formed by described Chinese medicine composition and the first weight ratio proportioning according to 55: 10 of symphysis; Described symphysis unit be by bacillus bifidus system and oligosaccharide according to 3: 10 weight ratio proportioning form, be 20,000,000,000 containing the number of active bifid bacteria in described every gram of symphysis unit, described oligosaccharide is oligomeric xylose.
Symphysis unit compositions containing above-mentioned Chinese medicine composition 2, is called for short symphysis unit compositions 2: formed by described Chinese medicine composition and the first weight ratio proportioning according to 90: 5 of symphysis; Described symphysis unit be by bacillus bifidus system and oligosaccharide according to 1: 1 weight ratio proportioning form, be 45,000,000,000 containing the number of active bifid bacteria in described every gram of symphysis unit, described oligosaccharide is fruit oligose.
Symphysis unit compositions containing above-mentioned Chinese medicine composition 3, is called for short symphysis unit compositions 3: formed by described Chinese medicine composition and the first weight ratio proportioning according to 80: 7 of symphysis; Described symphysis unit be by bifidobacterium preparations and oligosaccharide according to 5: 8 weight ratio proportioning form, be 40,000,000,000 containing the number of active bifid bacteria in described every gram of symphysis unit, described oligosaccharide is soybean oligo saccharide.
3 preparations being used for the treatment of the preparation of chickling intestinal coliform diarrhea
In the described symphysis unit compositions 3 of the bifidobacterium preparations prepare embodiment 1 and embodiment 3, the soybean oligo saccharide of formula ratio fully mixes, and obtains described symphysis unit; By the decocting for Chinese herbal medicine liquid mixing that the described symphysis unit of described symphysis unit compositions 3 formula ratio of embodiment 3 and described Chinese medicine composition obtain, obtain the preparation of symphysis unit compositions, in the preparation of described symphysis unit compositions, bacillus bifidus equals 1 × 10
7individual/mL.
Each component in said herbal medicine additive, (Xie Zongwan edits to refer to " national Chinese herbal medicine compilation ", People's Health Publisher, 1996-1052) and " Chinese medicine voluminous dictionary " (Nanjing University of Traditional Chinese Medicine writes, Shanghai science tech publishing house publication time: the 2006-3-1 second edition.
Embodiment 2 is used for the treatment of the effect of the preparation of chickling intestinal coliform diarrhea
The effect of one treatment chickling intestinal coliform diarrhea
In normal feedstuff, add the preparation of symphysis unit compositions prepared by embodiment 1, the feedstuff of the preparation of described symphysis unit compositions must be contained.Be the preparation of the described symphysis unit compositions of 8-15% containing mass fraction in described feedstuff.
1) 160 chickling (every chickling is independently raised), are divided into experimental group and matched group.Experimental group 80, mixes with 2000 grams of normal feedstuff with 280 grams, the preparation of above-mentioned symphysis unit compositions, feeds chickling 7 days.Matched group 80, feeds chickling 7 days with normal feedstuff.Wherein, there is not intestinal coliform diarrhea in experimental group chickling; The incidence rate of matched group coliform diarrhea is for being 20%.
20 chickling of 2 pairs of matched groups morbidity contain the standard feeding 5 days of the feedstuff of the preparation of described symphysis unit compositions by every 110g that only often eats, and 14 chickling coliform diarrheas are cured, and after this do not recur.
The diagnosis of chickling intestinal coliform diarrhea is civilian with reference to village a kind of jade " diagnosis of chickling Escherichia coli disease ".
Two feed effectiveness comparison
160 chickling of the same age, are divided into experimental group and matched group at random.Experimental group 80, mixes with 2000 grams of normal feedstuff with 160 grams, the preparation of above-mentioned symphysis unit compositions, feeds chickling 5 days.Matched group 80, feeds chickling 5 days with normal feedstuff.
After feeding completes for five days, record the daily inleting appetite that feeding completes the 2nd day, the 3rd day and the 5th day respectively.Daily inleting appetite be calculated as matched group or experimental group feed total amount divided by chickling quantity, i.e. the per day food-intake of every chickling.
The per day food-intake of table 1 chickling
What finally illustrate is, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although with reference to preferred embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not departing from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (8)
1. be used for the treatment of the preparation of chickling intestinal coliform diarrhea, it is characterized in that: formed according to the weight ratio proportioning of 55-90:5-10 by Chinese medicine composition and symphysis unit; Described Chinese medicine composition is made up of the dry medical material of following weight portion: Herba Dianthi 35-50 part, Radix Pulsatillae 3-8 part, Radix Glycyrrhizae 5-15 part, Herba Taraxaci 8-25 part; Described symphysis unit is formed according to the weight ratio proportioning of 3-5:5-10 by bifidobacterium preparations and oligosaccharide, containing the number of active bifid bacteria in described every gram of symphysis unit is 200-450 hundred million, and described oligosaccharide is any one or more in fruit oligose, oligomeric xylose, dextrinosan and soybean oligo saccharide.
2. the preparation being used for the treatment of chickling intestinal coliform diarrhea according to claim 1, is characterized in that: described Chinese medicine composition is made up of the dry medical material of following weight portion: Herba Dianthi 45 parts, the Radix Pulsatillae 6 parts, 13 parts, Radix Glycyrrhizae, Herba Taraxaci 22 parts.
3. the preparation being used for the treatment of chickling intestinal coliform diarrhea according to claim 1, is characterized in that: formed according to the weight ratio proportioning of 80:7 by described Chinese medicine composition and symphysis unit; Described symphysis unit is formed according to the weight ratio proportioning of 5:8 by bifidobacterium preparations and oligosaccharide, and containing the number of active bifid bacteria in described every gram of symphysis unit is 40,000,000,000, and described oligosaccharide is soybean oligo saccharide.
4. the preparation method of preparation according to claim 1, is characterized in that, specifically comprises the following steps: being inoculated by the 4-6% of bacillus bifidus by culture volume, is 6.0-8.0 at pH value, and 37 DEG C of constant temperature quiescent culture 24-72 hour, obtain bifidobacterium preparations; Medium of Bifidobacterium calculates composed of the following components by weight: peptone 0.1-1.0 part, Carnis Bovis seu Bubali cream 0.1-0.5 part, cysteine 0.1-0.2 part, sodium chloride 0.1-0.5 part, glucose 1-1.5 part, hepar siccatum 0.1-0.2 part, aqueous medium 90-99 part, sterilizing; The bifidobacterium preparations of formula ratio and the oligosaccharide of formula ratio are fully mixed, obtains described symphysis unit; By the decocting for Chinese herbal medicine liquid mixing that described symphysis unit and the described Chinese medicine composition of formula ratio obtain, obtain the preparation of symphysis unit compositions, in the preparation of described symphysis unit compositions, the concentration of bacillus bifidus is more than or equal to 1 × 10
7individual/mL.
5. preparation method according to claim 4, is characterized in that, described bifidobacterium preparations specifically comprises the following steps:
A expands kind of a daughter bacteria
Get strain inoculation on inclined-plane and enter to be equipped with Medium of Bifidobacterium in vitro, 37 DEG C of constant temperature quiescent culture 24 hours, obtain first order seed bacterium, stored refrigerated;
The preparation of B secondary seed bacterium
By the ratio of first order seed bacterium in the 4-6% of Medium of Bifidobacterium volume, be inoculated into sterilizing and cooling Medium of Bifidobacterium in, 37 DEG C of constant temperature quiescent culture 24h, obtain secondary seed bacterium; Stored refrigerated; Poured into by Medium of Bifidobacterium in microorganism fermentation tank, sterilizing also cools;
C fermentation culture
By step B gained secondary seed bacterium in sterilizing and the ratio inoculation of the 6-8% of the Medium of Bifidobacterium volume of cooling, stir, 37 DEG C of constant temperature quiescent culture 48-72h, obtain culture fluid;
D centrifuge dehydration
In the centrifugal 20min of 3000r/min after step C gained culture fluid is cooled, remove supernatant, get bacterium mud, add and be equivalent to bacterium mud weight 1-2 filler mix homogeneously doubly, dry under being placed in 40-50 DEG C of condition, obtain bifidobacterium preparations.
6. preparation method according to claim 5, is characterized in that: Medium of Bifidobacterium formula: peptone 0.6 part, Carnis Bovis seu Bubali cream 0.3 part, cysteine 0.15 part, 0.5 part, sodium chloride, glucose 1.2 parts, hepar siccatum 0.2 part, aqueous medium 95 parts.
7. containing the feedstuff being used for the treatment of the preparation of chickling intestinal coliform diarrhea according to claim 1.
8. feedstuff according to claim 7, is characterized in that, is the preparation of the described symphysis unit compositions of 8-15% in described feedstuff containing mass fraction.
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| CN105535387B (en) * | 2014-11-03 | 2018-05-08 | 孙文彬 | Animal and bird intestines diarrhea ferment and preparation method thereof can be improved |
| CN106074812B (en) * | 2016-08-29 | 2020-01-21 | 兴文县鸿程农业生态科技开发有限责任公司 | Traditional Chinese medicine compound for preventing and treating poultry bacterial diseases and application |
| CN108783076A (en) * | 2018-06-28 | 2018-11-13 | 浦北县清华生态养鸡专业合作社 | Prevent the preparation method of chicken colibacillosis feed |
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| 试论仔猪白痢病的发病规律及分类防治;裴忠绳等;《中兽医医药杂志》;20051231(第2期);第45-46页 * |
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