CN103168965B - Anti-stress synbiotics traditional Chinese medicine composition applicable to chicken and preparation method of anti-stress synbiotics traditional Chinese medicine composition - Google Patents
Anti-stress synbiotics traditional Chinese medicine composition applicable to chicken and preparation method of anti-stress synbiotics traditional Chinese medicine composition Download PDFInfo
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Abstract
The invention relates to the field of livestock breeding and particularly relates to an anti-stress synbiotics traditional Chinese medicine composition for chicken. The anti-stress synbiotics traditional Chinese medicine composition consists of a micro-ecological synbiotics feed additive and a Chinese herbal medicine additive, wherein the micro-ecological synbiotics feed additive consists of the following components in parts by weight: 10-60 parts of micro-ecological probiotics and 40-90 parts of oligosaccharide, 20-45 billion living bifidobacteria are contained in per gram of micro-ecological synbiotics feed additive, the micro-ecological probiotics are a bifidobacteria preparation, the oligosaccharide is any one or more of fructooligosaccharide, xylooligosaccharide, isomaltooligosacharide and soybean oligosaccharides, the micro-ecological synbiotics feed additive accounts for 1-2% of the total weight of the anti-stress synbiotics traditional Chinese medicine composition for chicken, and the Chinese herbal medicine additive consists of the following components in parts by weight: 3-5 parts of schisandra, 10-15 parts of keels, 15-18 parts of zizyphus jujube kernel, 10-20 parts of nymphaea tetragona and 25-30 parts of clover. The anti-stress synbiotics traditional Chinese medicine composition can be used for improving the immunity and anti-stress capability of the chicken, and reducing the incidence of diseases.
Description
Technical field
The present invention relates to livestock rearing field, particularly micro-ecological feed and preparation method thereof.
Background technology
What is called stress (stress) refer to a kind of nonspecific reaction that animal shows poor environment factor.These factors comprise: the flip-flop of living environment as transport, turn the crowded and trample etc. in group and when resettlement; The flip-flop of environmental condition is as the flip-flop of the variation of weather, light application time and intensity; The change of nutritional status is as the flip-flop of the kind of feed, quality, quantity, and feed formula is unreasonable, the long-term lacking of some nutritional labeling or excessive; The deterioration of living environment is as too high in cultivation density, harmful gas concentration is excessive, the dysdipsia of searching for food, water quality inferiority, ventilation is bad, dust is many, pathogenic microorganism is many, sanitary condition is poor etc.; The flip-flop of feeding and management program is as grabbed chicken, docking, injection, controlled feeding, suddenly clear excrement etc.; Disease is undoubtedly stress factor, and dispensing also easily cause stress.The phosphamidon class medical instrument of high dose has peculiar smell, excitant and toxicity, and mordant disinfectant easily causes for the drinking-water of feeding process, spraying, sterilization etc. stress; Stressor because of some vaccine and immunologic process thereof can cause stress.The unexpected variation of the unexpected movement of object, unexpected noise, work clothes color (particularly red) etc. all can cause that livestock and poultry are excited and produce stress.In fact, all can cause that livestock and poultry body does not feel like oneself and causes the factor of body damage all to can be described as stressor.
Chick is that a class is to the highstrung animal of environmental change.Chick, under stress situation, often shows the symptoms such as apocleisis, dysphoria, growth slow down, death rate rising.Generally take the pattern of high density culture because the uncontrollability of Changes in weather adds China raiser for pursuing cultured output, therefore, in breeding process there is in various degree stress phenomenon inevitable in chick.Due to stress, exceed 2,000,000,000 yuan to every year the direct economic loss brought of China's chick breeding industry.Therefore, develop a kind of effect significantly, stable, the chick Antistress agent tool easy to use of effect is of great significance.
At present, for livestock and poultry anti-stress medicine or additive, mostly be chemicals, long-term use, not only can cause that livestock and poultry body develops immunity to drugs, stress reaction, the bad reaction such as poisoning, and its harmful components residue in the livestock products such as meat, egg, milk, can directly be detrimental to health.Modern medicine study shows, in animal derived food, antibiotic residue may cause human DNA structure to be undergone mutation, thereby causes the serious consequences such as " disabling ", " teratogenesis ", " carcinogenic ".
Natural Chinese medicinal herb and probiotics preparation have no side effect with its uniqueness, and without hazard residue, the advantage such as non-stress, does not produce drug resistance, free from environmental pollution, becomes and produce the benefit-additives of green animal food and emphasis and the focus of herding worker research.External approved uses as feed medicated premix.Along with further developing of China's animal husbandry and animal products foreign trade.Must pay attention to the reasonable use of medicated premix.
Summary of the invention
The object of the present invention is to provide the composition with a kind of micro-ecological symphysis unit's feed addictive and Chinese herbal feed composition, the number of viable that described additive contains is high, the stress reaction of the Feed Energy treatment chick of preparing with this additive.
For achieving the above object, technical scheme of the present invention is:
Chick is anti-stress symphysis unit Chinese medicine composition, formed by micro-ecological symphysis unit's feed addictive and Chinese herb feed additive, the first feed addictive of micro-ecological symphysis is composed of the following components according to listed as parts by weight: micro-ecological probio 10-60 part, oligosaccharides 40-90 part, in every gram of described micro-ecological symphysis unit feed addictive, be hundred million of 200-450 containing the number of active bifid bacteria, described micro-ecological probio is bifidobacterium preparations, and described oligosaccharides is any one or more in FOS, xylo-oligosaccharide, isomalto-oligosaccharide and soyabean oligosaccharides; Described micro-ecological symphysis unit feed addictive accounts for the anti-1-2% that stress symphysis unit Chinese medicine composition gross weight of described chick, and described Chinese herb feed additive is composed of the following components by weight: fruit of Chinese magnoliavine 3-5 part, keel 10-15 part, spina date seed 15-18 part; Water lily 10-20 part; Clover 25-30 part.
Further, the first feed addictive of the micro-ecological symphysis of described Chinese herbal medicine, in the first feed addictive of described micro-ecological symphysis, every gram is 40,000,000,000 containing the number of active bifid bacteria.
Further, described chick is anti-stress symphysis unit Chinese medicine composition, described micro-ecological symphysis unit feed addictive account for described chick anti-stress symphysis unit Chinese medicine composition gross weight 1.5%, described Chinese herb feed additive is composed of the following components by weight: 5 parts, the fruit of Chinese magnoliavine, 12 parts of keel, 16 parts of spina date seeds; 18 parts of water lilys; 27 parts of clover.
Contain the anti-feed that stress symphysis unit Chinese medicine composition of described chick.
Further, in described feed, containing mass fraction is that the described chick of 1.5-2% is anti-stress symphysis unit Chinese medicine composition.
The preparation method who another object of the present invention is to provide the anti-preparation that stress symphysis unit Chinese medicine composition of above-mentioned chick, the method is simple to operate, and production cost is low, is applicable to large-scale production.
The preparation method of the anti-preparation that stress symphysis unit Chinese medicine composition of described chick, the 4-6% inoculation of Bifidobacterium press to culture volume, is 6.0-8.0 in pH value, the 37 DEG C of standing cultivation of constant temperature 24-72 hour, obtain bifidobacterium preparations; Medium of Bifidobacterium calculates composed of the following components by weight: peptone 0.1-1.0 part, beef extract 0.1-0.5 part, cysteine 0.1-0.2 part, sodium chloride 0.1-0.5 part, glucose 1-1.5 part, hepar siccatum 0.1-0.2 part, aqueous medium 90-99 part, sterilizing; The FOS of the bifidobacterium preparations of formula ratio and formula ratio is fully mixed, obtain the first feed addictive of micro-ecological symphysis; Described micro-ecological symphysis unit's feed addictive of formula ratio and each component of described Chinese herb feed additive are fully mixed, obtain the anti-preparation that stress symphysis unit Chinese medicine composition of chick.
Further, in described preparation method, described bifidobacterium preparations specifically comprises the following steps:
A expands kind of a daughter bacteria
Get bacterial classification on inclined-plane and inoculate into Medium of Bifidobacterium being housed in vitro, 37 DEG C of constant temperature leave standstill to be cultivated 24 hours, obtains first order seed bacterium, stored refrigerated stand-by;
The preparation of B secondary seed bacterium
First order seed bacterium, in the ratio of the 4-6% of Medium of Bifidobacterium volume, is inoculated in sterilizing cooling Medium of Bifidobacterium, and 37 DEG C of constant temperature leave standstill cultivates 24h, obtains secondary seed bacterium; Take out Refrigerator store, stand-by; Medium of Bifidobacterium is poured in microorganism fermentation tank, and sterilizing is also cooling;
C fermented and cultured
Step B gained secondary seed bacterium, in the also ratio inoculation of the 6-8% of cooling Medium of Bifidobacterium volume of sterilizing, is stirred, and 37 DEG C of constant temperature leave standstill cultivates 48-72h, obtains nutrient solution;
D centrifugal dehydration
By after cooling step C gained nutrient solution in the centrifugal 20min of 3000r/min, remove supernatant, get bacterium mud, add and be equivalent to bacterium mud weight 1-2 filler doubly and mix, be placed under 40-50 DEG C of condition and dry, obtain bifidobacterium preparations.
Further, in described preparation method, Medium of Bifidobacterium formula: 0.6 part of peptone, 0.3 part of beef extract, 0.15 part of cysteine, 0.5 part, sodium chloride, 1.2 parts of glucose, 0.2 part of hepar siccatum, 95 parts of aqueous mediums.
Beneficial effect of the present invention is: 1) compare and have the following advantages with alone ecological symphysis unit feed addictive: can improve chicken immunity and anti-stress ability, reduce the incidence of disease, the few or not medication of medication, is conducive to health of human body and environmental protection; Be applicable to modernization industry and produce, constant product quality; 2) Chinese herbal medicine in this formula, the power of fruit of Chinese magnoliavine energy strengthening by means of tonics, has tonifying liver effect; Keel have tranquillizing the mind by relieving convulsion, arrest sweating controlling nocturnal emission with astringent drugs, the puckery intestines that stop blooding, the effect of regenerating tissue to heal wond; Spina date seed has analgesia, anticonvulsion, cooling effect; Water lily has wet brain calms the nerves, and effect of hot nourishing the liver falls in heat-clearing bushing; Clover has heat-clearing, the effect of cool blood; Use in conjunction is obvious to chick anti-stress ability.
Detailed description of the invention
The anti-preparation that stress symphysis unit Chinese medicinal composition preparation of embodiment 1 chick
One, taking oligosaccharides is that the each 10Kg of FOS, xylo-oligosaccharide, isomalto-oligosaccharide and soyabean oligosaccharides is for subsequent use;
Two, the preparation of bifidobacterium preparations:
Bacterial classification is the Bifidobacterium (Bifidobacterium) that freeze-drying is preserved; Medium of Bifidobacterium calculates composed of the following components by weight: 0.6 part of peptone, 0.3 part of beef extract, 0.15 part of cysteine, 0.5 part, sodium chloride, 1.2 parts of glucose, 0.2 part of hepar siccatum, 95 parts of aqueous mediums, pH6.8,115 DEG C of high pressure steam sterilization 30min;
1. expand kind of a daughter bacteria, get bacterial classification on inclined-plane (as an oese) and inoculate into Medium of Bifidobacterium being housed in vitro, 37 DEG C of constant temperature leave standstill cultivates 24h, obtains first order seed bacterium, takes out Refrigerator store, stand-by;
2. the preparation of secondary seed bacterium: first order seed bacterium, in 5% ratio of the volume of Medium of Bifidobacterium, is inoculated in Bifidobacterium bacterium culture medium, and 37 DEG C of constant temperature leave standstill cultivates 24h, obtains secondary seed bacterium;
3. produce the preparation of the Medium of Bifidobacterium of use: take each composition in the composition ratio of Medium of Bifidobacterium and be dissolved in water, pour in microorganism fermentation tank, in 115 DEG C of steam sterilizations 30 minutes;
4. inoculation: the Medium of Bifidobacterium of use to be produced is chilled to 37 DEG C of left and right, adds secondary seed bacterium under flame control, in the ratio inoculation of the 6-8% of Medium of Bifidobacterium volume, stirs;
5. cultivate: close all breather valves, 37 DEG C of constant temperature culture 60h;
6. centrifugal dehydration: take out nutrient solution in biological fermentation tank, refrigerator is temporary, after nutrient solution is cooling in 3000 revs/min centrifugal 20 minutes, remove supernatant, getting bacterium mud adds 2 times of chaff powder to mix, pack disk into, be placed in 45 DEG C of air dry ovens and dry, carry out Bifidobacterium qualification by GB4789-2012 method, and calculate total viable count, pack, obtain bifidobacterium preparations, in cool place, dry place keeps in Dark Place for subsequent use.
The preparation of the anti-preparation that stress symphysis unit Chinese medicine composition of three chick
1) micro-ecological symphysis unit's feed addictive prescription one (being called for short additive 1): calculate by weight composed of the following components: 50 parts of bifidobacterium preparations, 90 parts of FOSs, wherein, in every gram of first feed addictive of ecological symphysis, viable count is 30,000,000,000.
Chick is anti-stress symphysis unit Chinese traditional medicine composition composition formula one (being called for short composition 1): additive 1 account for described chick anti-stress symphysis unit Chinese medicine composition 1 gross weight 1%, surplus is Chinese herb feed additive (being called for short Chinese herbal medicine 1), described Chinese herb feed additive is composed of the following components by weight: 3 parts, the fruit of Chinese magnoliavine, 15 parts of keel, 15 parts of spina date seeds; 20 parts of water lilys; 30 parts of clover.
2) micro-ecological symphysis unit's feed addictive prescription two (being called for short additive 2): calculate by weight composed of the following components: 60 parts of bifidobacterium preparations, 40 parts of soyabean oligosaccharides, wherein, in every gram of first feed addictive of ecological symphysis, viable count is 45,000,000,000.
Chick is anti-stress symphysis unit Chinese traditional medicine composition composition formula one (being called for short composition 2): additive 1 account for described chick anti-stress symphysis unit Chinese medicine composition 2 gross weights 2%, surplus is Chinese herb feed additive (being called for short Chinese herbal medicine 1), described Chinese herb feed additive is composed of the following components by weight: 5 parts, the fruit of Chinese magnoliavine, 10 parts of keel, 18 parts of spina date seeds; 10 parts of water lilys; 25 parts of clover.
3) micro-ecological symphysis unit's feed addictive prescription three (being called for short additive 3): calculate by weight composed of the following components: 20 parts of bifidobacterium preparations, 80 parts of xylo-oligosaccharides, wherein, in every gram of first feed addictive of ecological symphysis, viable count is 40,000,000,000.
Chick is anti-stress symphysis unit Chinese traditional medicine composition composition formula one (being called for short composition 3): additive 1 account for described chick anti-stress symphysis unit Chinese medicine composition 3 gross weights 1.5%, surplus is Chinese herb feed additive (being called for short Chinese herbal medicine 1), described Chinese herb feed additive is composed of the following components by weight: 5 parts, the fruit of Chinese magnoliavine, 12 parts of keel, 16 parts of spina date seeds; 18 parts of water lilys; 27 parts of clover.Mouthfeel the best of this formula.
Each component in said herbal medicine additive, refer to " national Chinese herbal medicine compilation " (Xie Zongwan chief editor, People's Health Publisher, 1996-1052) and " Chinese medicine voluminous dictionary " (Nanjing University of Traditional Chinese Medicine writes, Shanghai science tech publishing house publication time: the 2006-3-1 second edition.
Each component of formula ratio is mixed in proportion, and granulation agent, so that quality control, preservation, transport and use.
The application of the anti-preparation that stress symphysis unit Chinese medicine composition of embodiment 2 chick
One, experiment grouping
1 experimental group
Be chosen at 300 chick that occur transport stress reaction in transportation, the feed of freely ingesting and containing composition 2 described in embodiment 1.The mass percent of the anti-preparation that stress symphysis unit Chinese medicine composition of the chick that adds in described feed is 2%.
2 control groups
Be chosen at 50 chick that occur transport stress reaction in transportation, edible normal diet.
Two, index detects
Blood is got in blood sample acquisition method oxter, and blood sample is taked after respectively at first 1 day of administration, administration for 3 days.Every each blood sampling 0.5ml, separation of serum is put-20 DEG C of refrigerators, does Biochemical Indices In Serum inspection, in 4d, completes.
Biochemical investigation method Physiological-biochemical Parameters send laboratory, Rongchang County the People's Hospital to detect.
The serum glucose of experimental group and control group chick, serum cortisol, urea nitrogen assay are in table 1.From table 1, test group serum glucose, serum cortisol, urea nitrogen average are tending towards declining, and through t inspection, have utmost point significant difference (P < 0.01) with control group.
The variation of table 1 serum glucose, T4, urea nitrogen
Experimental group is continuously in the edible feed that contains composition 2 described in embodiment 1 one week.Control group and experimental group were under equal conditions raised respectively after January, and the death rate of control group is 23%, and the experimental group death rate is 4%.
Finally explanation is, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although the present invention is had been described in detail with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can modify or be equal to replacement technical scheme of the present invention, and not departing from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of claim scope of the present invention.
Claims (8)
1. chick is anti-stress symphysis unit Chinese medicine composition, it is characterized in that, formed by micro-ecological symphysis unit's feed addictive and Chinese herb feed additive, the first feed addictive of micro-ecological symphysis is composed of the following components according to listed as parts by weight: micro-ecological probio 10-60 part, oligosaccharides 40-90 part, in every gram of described micro-ecological symphysis unit feed addictive, be hundred million of 200-450 containing the number of active bifid bacteria, described micro-ecological probio is bifidobacterium preparations, and described oligosaccharides is any one or more in FOS, xylo-oligosaccharide, isomalto-oligosaccharide and soyabean oligosaccharides; Described micro-ecological symphysis unit feed addictive accounts for the anti-1-2% that stress symphysis unit Chinese medicine composition gross weight of described chick, and described Chinese herb feed additive is composed of the following components by weight: fruit of Chinese magnoliavine 3-5 part, keel 10-15 part, spina date seed 15-18 part; Water lily 10-20 part; Clover 25-30 part.
2. chick according to claim 1 is anti-stress symphysis unit Chinese medicine composition, it is characterized in that, in described micro-ecological symphysis unit feed addictive, every gram is 40,000,000,000 containing the number of active bifid bacteria.
3. chick according to claim 1 is anti-stress symphysis unit Chinese medicine composition, it is characterized in that: described micro-ecological symphysis unit feed addictive account for described chick anti-stress symphysis unit Chinese medicine composition gross weight 1.5%, described Chinese herb feed additive is composed of the following components by weight: 5 parts, the fruit of Chinese magnoliavine, 12 parts of keel, 16 parts of spina date seeds; 18 parts of water lilys; 27 parts of clover.
4. the preparation method of the anti-preparation that stress symphysis unit Chinese medicine composition of the chick described in claim 1-3 any one, it is characterized in that, Bifidobacterium is pressed to the 4-6% inoculation of culture volume, is 6.0-8.0 in pH value, 37 DEG C of constant temperature leave standstill cultivates 24-72 hour, obtains bifidobacterium preparations; Medium of Bifidobacterium calculates composed of the following components by weight: peptone 0.1-1.0 part, beef extract 0.1-0.5 part, cysteine 0.1-0.2 part, sodium chloride 0.1-0.5 part, glucose 1-1.5 part, hepar siccatum 0.1-0.2 part, aqueous medium 90-99 part, sterilizing; The FOS of the bifidobacterium preparations of formula ratio and formula ratio is fully mixed, obtain the first feed addictive of micro-ecological symphysis; Described micro-ecological symphysis unit's feed addictive of formula ratio and each component of described Chinese herb feed additive are fully mixed, obtain the anti-preparation that stress symphysis unit Chinese medicine composition of chick.
5. preparation method according to claim 4, is characterized in that, described bifidobacterium preparations specifically comprises the following steps:
A expands kind of a daughter bacteria
Get bacterial classification on inclined-plane and inoculate into Medium of Bifidobacterium being housed in vitro, 37 DEG C of constant temperature leave standstill to be cultivated 24 hours, obtains first order seed bacterium, stored refrigerated;
The preparation of B secondary seed bacterium
First order seed bacterium, in the ratio of the 4-6% of Medium of Bifidobacterium volume, is inoculated in sterilizing cooling Medium of Bifidobacterium, and 37 DEG C of constant temperature leave standstill cultivates 24h, obtains secondary seed bacterium; Stored refrigerated; Medium of Bifidobacterium is poured in microorganism fermentation tank, and sterilizing is also cooling;
C fermented and cultured
Step B gained secondary seed bacterium, in the also ratio inoculation of the 6-8% of cooling Medium of Bifidobacterium volume of sterilizing, is stirred, and 37 DEG C of constant temperature leave standstill cultivates 48-72h, obtains nutrient solution;
D centrifugal dehydration
By after cooling step C gained nutrient solution in the centrifugal 20min of 3000r/min, remove supernatant, get bacterium mud, add and be equivalent to bacterium mud weight 1-2 filler doubly and mix, be placed under 40-50 DEG C of condition and dry, obtain bifidobacterium preparations.
6. preparation method according to claim 5, is characterized in that: Medium of Bifidobacterium formula: 0.6 part of peptone, 0.3 part of beef extract, 0.15 part of cysteine, 0.5 part, sodium chloride, 1.2 parts of glucose, 0.2 part of hepar siccatum, 95 parts of aqueous mediums.
7. contain the anti-feed that stress symphysis unit Chinese medicine composition of chick claimed in claim 1.
8. feed according to claim 7, is characterized in that, in described feed, contain mass fraction and be the described chick of 1.5-2% is anti-stress symphysis unit Chinese medicine composition.
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| CN103392936B (en) * | 2013-08-05 | 2014-10-22 | 山东新希望六和集团有限公司 | Compound feed for broilers at age between 1 day and 42 days and preparation method thereof |
| CN104886356A (en) * | 2014-03-05 | 2015-09-09 | 甘肃康源生物科技有限公司 | Animal stress-resistant Chinese herbal medicine additive and preparation method thereof |
| CN104719641B (en) * | 2015-03-15 | 2018-05-22 | 湖南农业大学 | A kind of preparation method of livestock and poultry anti-stress feed addictive |
| CN104799121B (en) * | 2015-05-18 | 2017-12-26 | 武汉中拓康明生物科技有限公司 | It is a kind of be used for improve chick high temperature resistance stress feed addictive |
| CN104799120B (en) * | 2015-05-18 | 2018-03-13 | 广东科润生物制药有限公司 | A kind of feed addictive for being used to improve chick low-temperature resistance stress |
| CN105477225B (en) * | 2015-12-18 | 2021-08-13 | 河北农业大学 | Traditional Chinese veterinary medicine for enhancing anti-stress capability of chicks, preparation method and feeding method |
| CN106666195A (en) * | 2016-12-20 | 2017-05-17 | 钦州市康绿宝农业有限公司 | Chick feed additive and preparation method thereof |
| CN107173622A (en) * | 2017-06-21 | 2017-09-19 | 安徽省金安禽业有限公司 | A kind of nutritious resisting stress egg feedstuff |
| CN108323622A (en) * | 2018-02-11 | 2018-07-27 | 四川达邦生物科技有限公司 | A kind of preparation method of the feed addictive containing composite vegetables extractive |
| CN108157664A (en) * | 2018-02-11 | 2018-06-15 | 四川达邦生物科技有限公司 | A kind of feed addictive containing composite vegetables extractive and its preparation process for being used to improve poultry immunity |
| CN108740306A (en) * | 2018-05-03 | 2018-11-06 | 武汉环绿时代生态园林工程有限公司 | A kind of feed addictive and its feeding method |
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| CN101338295B (en) * | 2008-08-09 | 2010-08-11 | 甘肃省科学院生物研究所 | Bacillus bifidus freeze concentration leaven and method for preparing same |
| CN102511662A (en) * | 2012-01-05 | 2012-06-27 | 上海创博生态工程有限公司 | Composite probiotic microorganism feed additive, preparation method and application thereof |
| CN102599345B (en) * | 2012-01-13 | 2013-07-31 | 山东华牧天元农牧股份有限公司 | Multicomponent biological compound agent for livestock |
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