CN105918629A - Use of traditional Chinese medicine fermented product as antibiotic substitute in feed - Google Patents
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Abstract
The invention discloses a use of a traditional Chinese medicine fermented product as an antibiotic substitute in feed. The use method comprises weighing 25-35 parts of pilose asiabell root, 10-20 parts of wolfberry, 15-25 parts of medicinal cyathula root and 25-35 parts of spina date seeds, mixing the materials, crushing the mixture to obtain traditional Chinese medicine powder, carrying out sterilization, preparing a culture solution containing, by mass, 0.2-0.4% of starch, 0.6-0.8% of ammonium sulfate, 0.2-0.4% of dipotassium hydrogen phosphate, 0.1-0.2% of potassium dihydrogen phosphate, 0.05-0.07% of magnesium sulfate, 0.04-0.06% of calcium chloride and 0.02-0.04% of yeast extract paste, adjusting pH to 7.0-7.2, carrying out sterilization, mixing the traditional Chinese medicine powder and the culture solution according to a weight ratio of 1: 40-50 to obtain a mixed culture medium, adding a compound Hiiranlactone A into the mixed culture medium so that a concentration is in a range of 50-60 micrograms per gram, inoculating the mixed culture medium with 4-6% of bacillus subtilis, carrying out fermentation cultivation for 24-48h, controlling pH to 7.0-7.2, and carrying out drying at a low temperature until water content is in a range of 6-8% to obtain the traditional Chinese medicine fermented product. The traditional Chinese medicine fermented product can significantly improve fattening sheep growth performances.
Description
Technical field
The present invention relates to antibiotic substitute, be specifically related to a kind of herb fermenting product as antibiotic substitute application in feedstuff.
Background technology
Antibiotic medicine from be born so far, once saved countless life with the power of its eliminating bacteria, will infect or
Light or the various diseases of weight patients save out from misery.But excessively use antibiotic can cause pathogenic bacteria drug resistance enhancing etc.
A little row problems.Currently, antibiotic overuse is a global medical science, society and public health problem.Antibiotic is applied
In the feedstuff industry history of existing about 50 years, the development for animal husbandry has played great impetus, in order to prevent disease and carry
High economic benefit, widespread practice is intended in feedstuff add antibiotic and chemosynthesis class medicine.Have the most in the world more than 20
Plant antibiotic and ten several synthetic antimicrobial class medicines are applied in feedstuff.But along with improving constantly of people's level of understanding, people
Find the most unsurmountable drawback after the great economic benefit that antibiotic is brought.Such as bacterial drug resistance strengthens, resists
Raw element relies on, antibiotic causes autogenous infection, superinfection, the decline of animal immunizing power and poultry and Aquatic product product and
The problems such as the antibiotic remains in environment all bring negative effect to feed industry, aquaculture and human health.In view of antibiotic
Adverse effect, research worker is devoted to develop some always and can strengthen animal immunizing power, increase economic efficiency and protect the mankind
Substitutes For Antibiotic with Environmental security.
Summary of the invention
It is an object of the invention to provide a kind of herb fermenting product and as antibiotic substitute application in feedstuff.
The above-mentioned purpose of the present invention is achieved by techniques below scheme:
A kind of herb fermenting product, are made by the steps and form:
Step S1, prepares Chinese medicinal powder: weigh Radix Codonopsis 25～35 parts, Fructus Lycii 10～20 parts, Radix Cyathulae 15～25 parts, Semen Ziziphi Spinosae
25～35 parts, co-grinding, obtain Chinese medicinal powder, sterilizing is standby；
Step S2, prepares culture fluid, starch-containing 0.2～0.4%, ammonium sulfate 0.6～0.8%, dipotassium hydrogen phosphate 0.2～0.4%, phosphorus
Acid dihydride potassium 0.1～0.2%, magnesium sulfate 0.05～0.07%, calcium chloride 0.04～0.06%, yeast extract 0.02～0.04%, be matter
Amount percentage composition；Regulation pH value is 7.0～7.2, and sterilizing is standby；
Step S3, is mixed to get mixed culture by the Chinese medicinal powder of above-mentioned sterilizing and the culture fluid of sterilizing according to weight ratio 1:40～50
Base, first adds compound HiiranlactoneA so that it is the concentration in mixed culture medium is 50～60 μ g/g, then by 4～6%
Inoculum concentration accesses fermentation of bacillus subtilis and cultivates 24～48 hours, and control ph is 7.0～7.2；
Step S4, oven drying at low temperature, to moisture 6～8%, obtains herb fermenting product.
Further, described herb fermenting product are made by the steps and form:
Step S1, prepares Chinese medicinal powder: weigh Radix Codonopsis 30 parts, Fructus Lycii 15 parts, Radix Cyathulae 20 parts, Semen Ziziphi Spinosae 30 parts, mixed
Closing and pulverize, obtain Chinese medicinal powder, sterilizing is standby；
Step S2, prepares culture fluid, starch-containing 0.3%, ammonium sulfate 0.7%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.15%,
Magnesium sulfate 0.06%, calcium chloride 0.05%, yeast extract 0.03%, be weight/mass percentage composition；Regulation pH value is 7.0～7.2,
Sterilizing is standby；
Step S3, is mixed to get mixed culture medium by the Chinese medicinal powder of above-mentioned sterilizing and the culture fluid of sterilizing according to weight ratio 1:45,
First add compound HiiranlactoneA so that it is the concentration in mixed culture medium is 55 μ g/g, then access by the inoculum concentration of 5%
Fermentation of bacillus subtilis is cultivated 36 hours, and control ph is 7.0～7.2；
Step S4, oven drying at low temperature, to moisture 6～8%, obtains herb fermenting product.
Further, described herb fermenting product are made by the steps and form:
Step S1, prepares Chinese medicinal powder: weigh Radix Codonopsis 25 parts, Fructus Lycii 10 parts, Radix Cyathulae 15 parts, Semen Ziziphi Spinosae 25 parts, mixed
Closing and pulverize, obtain Chinese medicinal powder, sterilizing is standby；
Step S2, prepares culture fluid, starch-containing 0.2%, ammonium sulfate 0.6%, dipotassium hydrogen phosphate 0.2%, potassium dihydrogen phosphate 0.1%,
Magnesium sulfate 0.05%, calcium chloride 0.04%, yeast extract 0.02%, be weight/mass percentage composition；Regulation pH value is 7.0～7.2,
Sterilizing is standby；
Step S3, is mixed to get mixed culture medium by the Chinese medicinal powder of above-mentioned sterilizing and the culture fluid of sterilizing according to weight ratio 1:40,
First add compound HiiranlactoneA so that it is the concentration in mixed culture medium is 50 μ g/g, then access by the inoculum concentration of 4%
Fermentation of bacillus subtilis is cultivated 48 hours, and control ph is 7.0～7.2；
Step S4, oven drying at low temperature, to moisture 6～8%, obtains herb fermenting product.
Further, described herb fermenting product are made by the steps and form:
Step S1, prepares Chinese medicinal powder: weigh Radix Codonopsis 35 parts, Fructus Lycii 20 parts, Radix Cyathulae 25 parts, Semen Ziziphi Spinosae 35 parts, mixed
Closing and pulverize, obtain Chinese medicinal powder, sterilizing is standby；
Step S2, prepares culture fluid, starch-containing 0.4%, ammonium sulfate 0.8%, dipotassium hydrogen phosphate 0.4%, potassium dihydrogen phosphate 0.2%,
Magnesium sulfate 0.07%, calcium chloride 0.06%, yeast extract 0.04%, be weight/mass percentage composition；Regulation pH value is 7.0～7.2,
Sterilizing is standby；
Step S3, is mixed to get mixed culture medium by the Chinese medicinal powder of above-mentioned sterilizing and the culture fluid of sterilizing according to weight ratio 1:50,
First add compound HiiranlactoneA so that it is the concentration in mixed culture medium is 60 μ g/g, then access by the inoculum concentration of 6%
Fermentation of bacillus subtilis is cultivated 24 hours, and control ph is 7.0～7.2；
Step S4, oven drying at low temperature, to moisture 6～8%, obtains herb fermenting product.
Above-mentioned herb fermenting product are as the purposes of feed additive.
Advantages of the present invention:
The herb fermenting product that the present invention provides can be added in feedstuff with substitute antibiotics, significantly improves the growth performance of domestic animal.
Detailed description of the invention
Further illustrate the essentiality content of the present invention below in conjunction with embodiment, but do not limit scope with this.To the greatest extent
The present invention is explained in detail by pipe with reference to preferred embodiment, it will be understood by those within the art that, can be to the present invention
Technical scheme modify or equivalent, without deviating from the spirit and scope of technical solution of the present invention.
The preparation method of compound Hiiranlactone A sees document: Secondary metabolites from the leaves of
Neolitsea hiiranensis and the anti-inflammatory activity of some of them,Phytochemistry,72
(2011),415–422。
Embodiment 1: the preparation of herb fermenting product
Step S1, prepares Chinese medicinal powder: weigh Radix Codonopsis 30 parts, Fructus Lycii 15 parts, Radix Cyathulae 20 parts, Semen Ziziphi Spinosae 30 parts, mixed
Closing and pulverize, obtain Chinese medicinal powder, sterilizing is standby；
Step S2, prepares culture fluid, starch-containing 0.3%, ammonium sulfate 0.7%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.15%,
Magnesium sulfate 0.06%, calcium chloride 0.05%, yeast extract 0.03%, be weight/mass percentage composition；Regulation pH value is 7.0～7.2,
Sterilizing is standby；
Step S3, is mixed to get mixed culture medium by the Chinese medicinal powder of above-mentioned sterilizing and the culture fluid of sterilizing according to weight ratio 1:45,
First add compound HiiranlactoneA so that it is the concentration in mixed culture medium is 55 μ g/g, then access by the inoculum concentration of 5%
Fermentation of bacillus subtilis is cultivated 36 hours, and control ph is 7.0～7.2；
Step S4, oven drying at low temperature, to moisture 6～8%, obtains herb fermenting product.
Embodiment 2: the preparation of herb fermenting product
Step S1, prepares Chinese medicinal powder: weigh Radix Codonopsis 25 parts, Fructus Lycii 10 parts, Radix Cyathulae 15 parts, Semen Ziziphi Spinosae 25 parts, mixed
Closing and pulverize, obtain Chinese medicinal powder, sterilizing is standby；
Step S2, prepares culture fluid, starch-containing 0.2%, ammonium sulfate 0.6%, dipotassium hydrogen phosphate 0.2%, potassium dihydrogen phosphate 0.1%,
Magnesium sulfate 0.05%, calcium chloride 0.04%, yeast extract 0.02%, be weight/mass percentage composition；Regulation pH value is 7.0～7.2,
Sterilizing is standby；
Step S3, is mixed to get mixed culture medium by the Chinese medicinal powder of above-mentioned sterilizing and the culture fluid of sterilizing according to weight ratio 1:40,
First add compound HiiranlactoneA so that it is the concentration in mixed culture medium is 50 μ g/g, then access by the inoculum concentration of 4%
Fermentation of bacillus subtilis is cultivated 48 hours, and control ph is 7.0～7.2；
Step S4, oven drying at low temperature, to moisture 6～8%, obtains herb fermenting product.
Embodiment 3: the preparation of herb fermenting product
Step S1, prepares Chinese medicinal powder: weigh Radix Codonopsis 35 parts, Fructus Lycii 20 parts, Radix Cyathulae 25 parts, Semen Ziziphi Spinosae 35 parts, mixed
Closing and pulverize, obtain Chinese medicinal powder, sterilizing is standby；
Step S2, prepares culture fluid, starch-containing 0.4%, ammonium sulfate 0.8%, dipotassium hydrogen phosphate 0.4%, potassium dihydrogen phosphate 0.2%,
Magnesium sulfate 0.07%, calcium chloride 0.06%, yeast extract 0.04%, be weight/mass percentage composition；Regulation pH value is 7.0～7.2,
Sterilizing is standby；
Step S3, is mixed to get mixed culture medium by the Chinese medicinal powder of above-mentioned sterilizing and the culture fluid of sterilizing according to weight ratio 1:50,
First add compound HiiranlactoneA so that it is the concentration in mixed culture medium is 60 μ g/g, then access by the inoculum concentration of 6%
Fermentation of bacillus subtilis is cultivated 24 hours, and control ph is 7.0～7.2；
Step S4, oven drying at low temperature, to moisture 6～8%, obtains herb fermenting product.
Embodiment 4: the preparation of herb fermenting product
Step S1, prepares Chinese medicinal powder: weigh Radix Codonopsis 30 parts, Fructus Lycii 15 parts, Radix Cyathulae 20 parts, Semen Ziziphi Spinosae 30 parts, mixed
Closing and pulverize, obtain Chinese medicinal powder, sterilizing is standby；
Step S2, prepares culture fluid, starch-containing 0.3%, ammonium sulfate 0.7%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.15%,
Magnesium sulfate 0.06%, calcium chloride 0.05%, yeast extract 0.03%, be weight/mass percentage composition；Regulation pH value is 7.0～7.2,
Sterilizing is standby；
Step S3, is mixed to get mixed culture medium by the Chinese medicinal powder of above-mentioned sterilizing and the culture fluid of sterilizing according to weight ratio 1:45,
First add compound HiiranlactoneA so that it is the concentration in mixed culture medium is 50 μ g/g, then access by the inoculum concentration of 5%
Fermentation of bacillus subtilis is cultivated 36 hours, and control ph is 7.0～7.2；
Step S4, oven drying at low temperature, to moisture 6～8%, obtains herb fermenting product.
Embodiment 5: the preparation of herb fermenting product
Step S1, prepares Chinese medicinal powder: weigh Radix Codonopsis 30 parts, Fructus Lycii 15 parts, Radix Cyathulae 20 parts, Semen Ziziphi Spinosae 30 parts, mixed
Closing and pulverize, obtain Chinese medicinal powder, sterilizing is standby；
Step S2, prepares culture fluid, starch-containing 0.3%, ammonium sulfate 0.7%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.15%,
Magnesium sulfate 0.06%, calcium chloride 0.05%, yeast extract 0.03%, be weight/mass percentage composition；Regulation pH value is 7.0～7.2,
Sterilizing is standby；
Step S3, is mixed to get mixed culture medium by the Chinese medicinal powder of above-mentioned sterilizing and the culture fluid of sterilizing according to weight ratio 1:45,
First add compound HiiranlactoneA so that it is the concentration in mixed culture medium is 60 μ g/g, then access by the inoculum concentration of 5%
Fermentation of bacillus subtilis is cultivated 36 hours, and control ph is 7.0～7.2；
Step S4, oven drying at low temperature, to moisture 6～8%, obtains herb fermenting product.
Embodiment 6: the contrast of embodiment 1, without compound HiiranlactoneA
Step S1, prepares Chinese medicinal powder: weigh Radix Codonopsis 30 parts, Fructus Lycii 15 parts, Radix Cyathulae 20 parts, Semen Ziziphi Spinosae 30 parts, mixed
Closing and pulverize, obtain Chinese medicinal powder, sterilizing is standby；
Step S2, prepares culture fluid, starch-containing 0.3%, ammonium sulfate 0.7%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.15%,
Magnesium sulfate 0.06%, calcium chloride 0.05%, yeast extract 0.03%, be weight/mass percentage composition；Regulation pH value is 7.0～7.2,
Sterilizing is standby；
Step S3, is mixed to get mixed culture medium by the Chinese medicinal powder of above-mentioned sterilizing and the culture fluid of sterilizing according to weight ratio 1:45,
Inoculum concentration by 5% accesses fermentation of bacillus subtilis and cultivates 36 hours, and control ph is 7.0～7.2；
Step S4, oven drying at low temperature, to moisture 6～8%, obtains herb fermenting product.
Embodiment 7: effect example
One, materials and methods
1, material: prepare herb fermenting product 1～6 according to the method for embodiment 1～6 respectively.
2, method
2.1 experimental animals and packet
Select the first-filial generation sheep 42 of 3 monthly age northeast thin-wool sheep × German Mutton merino, be randomly divided into 7 groups, often organize 6
Head, male and female half and half.Matched group uses Basic drawing to feed.Test group 1～6 is added respectively on the basis of Basic drawing respectively
The herb fermenting product 1～6 of embodiment 1～6 preparation of 600mg/kg.
2.2 method for breeding
60 days test periods.Use routine to raise and immunity during whole test, free choice feeding, freely drink water.
2.3 test indexs and method
Growth performance: statistics initial weight, end weight, Average weight increasing a day and feed-weight ratio.
3, statistical analysis
Test data uses SPSS20.0 software to carry out variance analysis, and the significance of difference carries out Duncans multiple comparisons, number in table
Value is expressed as Mean ± SD.
Two, result
The results are shown in Table 1.
As shown in Table 1, the Average weight increasing a day of test group 14,5 is above matched group, and feed-weight ratio is below matched group, with matched group
Compare significant difference (P ＜ 0.05)；The Average weight increasing a day of test group 6 and matched group there was no significant difference (P ＞ 0.05), feed-weight ratio
(P ＞ 0.05) is there was no significant difference with matched group.
The impact on Fattening Sheep growth performance of table 1 extract
The technique effect of test group 2,3 is basically identical with the technique effect of test group 4,5.
The above results shows, the herb fermenting product that the present invention provides add the growth performance being remarkably improved Fattening Sheep in feedstuff to,
This effect may with similar antibiotic is relevant.
The effect of above-described embodiment indicates that the essentiality content of the present invention, but does not limit protection scope of the present invention with this.
It will be understood by those within the art that, technical scheme can be modified or equivalent, and not take off
Essence and protection domain from technical solution of the present invention.
Claims (5)
1. herb fermenting product, it is characterised in that be made by the steps and form:
Step S1, prepares Chinese medicinal powder: weigh Radix Codonopsis 25～35 parts, Fructus Lycii 10～20 parts, Radix Cyathulae 15～25 parts, Semen Ziziphi Spinosae
25～35 parts, co-grinding, obtain Chinese medicinal powder, sterilizing is standby；
Step S2, prepares culture fluid, starch-containing 0.2～0.4%, ammonium sulfate 0.6～0.8%, dipotassium hydrogen phosphate 0.2～0.4%, phosphorus
Acid dihydride potassium 0.1～0.2%, magnesium sulfate 0.05～0.07%, calcium chloride 0.04～0.06%, yeast extract 0.02～0.04%, be matter
Amount percentage composition；Regulation pH value is 7.0～7.2, and sterilizing is standby；
Step S3, is mixed to get mixed culture by the Chinese medicinal powder of above-mentioned sterilizing and the culture fluid of sterilizing according to weight ratio 1:40～50
Base, first adds compound Hiiranlactone A so that it is the concentration in mixed culture medium is 50～60 μ g/g, then by 4～6%
Inoculum concentration accesses fermentation of bacillus subtilis and cultivates 24～48 hours, and control ph is 7.0～7.2；
Step S4, oven drying at low temperature, to moisture 6～8%, obtains herb fermenting product.
Herb fermenting product the most according to claim 1, it is characterised in that be made by the steps and form:
Step S1, prepares Chinese medicinal powder: weigh Radix Codonopsis 30 parts, Fructus Lycii 15 parts, Radix Cyathulae 20 parts, Semen Ziziphi Spinosae 30 parts, mixed
Closing and pulverize, obtain Chinese medicinal powder, sterilizing is standby；
Step S2, prepares culture fluid, starch-containing 0.3%, ammonium sulfate 0.7%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.15%,
Magnesium sulfate 0.06%, calcium chloride 0.05%, yeast extract 0.03%, be weight/mass percentage composition；Regulation pH value is 7.0～7.2,
Sterilizing is standby；
Step S3, is mixed to get mixed culture medium by the Chinese medicinal powder of above-mentioned sterilizing and the culture fluid of sterilizing according to weight ratio 1:45,
First add compound HiiranlactoneA so that it is the concentration in mixed culture medium is 55 μ g/g, then access by the inoculum concentration of 5%
Fermentation of bacillus subtilis is cultivated 36 hours, and control ph is 7.0～7.2；
Step S4, oven drying at low temperature, to moisture 6～8%, obtains herb fermenting product.
Herb fermenting product the most according to claim 1, it is characterised in that be made by the steps and form:
Step S1, prepares Chinese medicinal powder: weigh Radix Codonopsis 25 parts, Fructus Lycii 10 parts, Radix Cyathulae 15 parts, Semen Ziziphi Spinosae 25 parts, mixed
Closing and pulverize, obtain Chinese medicinal powder, sterilizing is standby；
Step S2, prepares culture fluid, starch-containing 0.2%, ammonium sulfate 0.6%, dipotassium hydrogen phosphate 0.2%, potassium dihydrogen phosphate 0.1%,
Magnesium sulfate 0.05%, calcium chloride 0.04%, yeast extract 0.02%, be weight/mass percentage composition；Regulation pH value is 7.0～7.2,
Sterilizing is standby；
Step S3, is mixed to get mixed culture medium by the Chinese medicinal powder of above-mentioned sterilizing and the culture fluid of sterilizing according to weight ratio 1:40,
First add compound HiiranlactoneA so that it is the concentration in mixed culture medium is 50 μ g/g, then access by the inoculum concentration of 4%
Fermentation of bacillus subtilis is cultivated 48 hours, and control ph is 7.0～7.2；
Step S4, oven drying at low temperature, to moisture 6～8%, obtains herb fermenting product.
Herb fermenting product the most according to claim 1, it is characterised in that be made by the steps and form:
Step S1, prepares Chinese medicinal powder: weigh Radix Codonopsis 35 parts, Fructus Lycii 20 parts, Radix Cyathulae 25 parts, Semen Ziziphi Spinosae 35 parts, mixed
Closing and pulverize, obtain Chinese medicinal powder, sterilizing is standby；
Step S2, prepares culture fluid, starch-containing 0.4%, ammonium sulfate 0.8%, dipotassium hydrogen phosphate 0.4%, potassium dihydrogen phosphate 0.2%,
Magnesium sulfate 0.07%, calcium chloride 0.06%, yeast extract 0.04%, be weight/mass percentage composition；Regulation pH value is 7.0～7.2,
Sterilizing is standby；
Step S3, is mixed to get mixed culture medium by the Chinese medicinal powder of above-mentioned sterilizing and the culture fluid of sterilizing according to weight ratio 1:50,
First add compound HiiranlactoneA so that it is the concentration in mixed culture medium is 60 μ g/g, then access by the inoculum concentration of 6%
Fermentation of bacillus subtilis is cultivated 24 hours, and control ph is 7.0～7.2；
Step S4, oven drying at low temperature, to moisture 6～8%, obtains herb fermenting product.
5. the arbitrary described herb fermenting product of Claims 1 to 4 are as the purposes of feed additive.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107996822A (en) * | 2017-11-10 | 2018-05-08 | 湖北华大瑞尔科技有限公司 | A kind of fermenting plant feed addictive of substitute antibiotics and preparation method thereof |
| CN108125021A (en) * | 2017-11-10 | 2018-06-08 | 湖北华大瑞尔科技有限公司 | A kind of feed additive for controlling diarrhea rate and preparation method thereof |
| CN108464390A (en) * | 2018-04-16 | 2018-08-31 | 苏州绿野田源农业科技有限公司 | A kind of dregs of beans biological fermentation feed and its production method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105360609A (en) * | 2015-10-22 | 2016-03-02 | 无锡中科活力生物技术有限公司 | Fodder premix and preparation method thereof |
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2016
- 2016-04-27 CN CN201610272271.1A patent/CN105918629A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105360609A (en) * | 2015-10-22 | 2016-03-02 | 无锡中科活力生物技术有限公司 | Fodder premix and preparation method thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107996822A (en) * | 2017-11-10 | 2018-05-08 | 湖北华大瑞尔科技有限公司 | A kind of fermenting plant feed addictive of substitute antibiotics and preparation method thereof |
| CN108125021A (en) * | 2017-11-10 | 2018-06-08 | 湖北华大瑞尔科技有限公司 | A kind of feed additive for controlling diarrhea rate and preparation method thereof |
| CN107996822B (en) * | 2017-11-10 | 2021-03-23 | 湖北华大瑞尔科技有限公司 | Fermented plant feed additive for replacing antibiotics and preparation method thereof |
| CN108464390A (en) * | 2018-04-16 | 2018-08-31 | 苏州绿野田源农业科技有限公司 | A kind of dregs of beans biological fermentation feed and its production method |
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