CN105360609A - Fodder premix and preparation method thereof - Google Patents
Fodder premix and preparation method thereof Download PDFInfo
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Abstract
The invention discloses fodder premix and a preparation method thereof. The preparation method comprises the steps that lactobacillus plantarum BW2013 strains, bacillus amyloliquefaciens A3 strains, bacillus substilis szx11 strains, rhodobacter sphaeroides WD-2 strains and baker's yeast are adopted as fermentation strains, a compound culture solution formed by compounding purslane extract, hairyvein agrimony extract, milkvetch root extract, humifuse euphorbia herb extract and licorice extract is adopted as a culture medium, fermentation is carried out, and the fodder premix with the effective viable count larger than or equal to 5*108 CFU/ml is prepared.
Description
Technical field
The present invention relates to microbe application and feed preblending agent modulation processing technique field, particularly relate to a kind of for improving Penaeus Vannmei immunity, improving enteron aisle disease, reduce syndromic feed preblending agent of Penaeus Vannmei Deaths and preparation method thereof.
Background technology
The total cultured area of the Penaeus Vannmei fresh water of China in 2005, seawater 1,500,000 mu, to 2010 namely more than 3,000,000 mu, annual production 90-100 ten thousand tons.The specialty that culture of Penaeus vannamei is no longer southern four provinces is started to 2012; North extend to the coastal areas such as Jiangsu and Zhejiang Provinces, Shandong, Hebei, Liaoning and develop more than 270 ten thousand mu rapidly, annual production reaches more than 1,500,000 tons.Since 2011, the disease death rate of culture of Penaeus vannamei is more and more higher, and rate is formed below 20% in most of area; To the first half of the year in 2012 in Hainan, the ground cultivation success rate such as Guangdong and Guangxi drops to about 10%, brings massive losses to white shrimp aquaculture.Its main cause be enteritis owing to propagating Penaeus Vannmei artificially, white just, dry Pancreatic Necrosis disease gets more and more, the Deaths syndrome (EMS) particularly constantly spread in recent years carrys out extreme loss to culture of Penaeus vannamei industrial belt.
Summary of the invention
In view of this, the object of the invention is to propose a kind of feed preblending agent and preparation method thereof, to improve the immune level of prawn, intestinal health, optimization water ecology, to reduce the incidence of disease of prawn, thus improve culture benefit.
Based on above-mentioned purpose, the preparation method of feed preblending agent provided by the invention comprises: with Lactobacillus plantarum LactobacillusplantarumBW2013 bacterial strain, bacillus amyloliquefaciens BacillusamyloliquefaciensA3 bacterial strain, Bacillus subtilis szx11 bacterial strain, hydrogenlike silicon ion RhodobactersphaeroidesWD-2 bacterial strain and bread microzyme are fermented bacterium, with purslane extract, Herba Agrimoniae extract, Astragalus Root P.E, Herba Euphorbiae Humifusae extract, the complex culture medium that licorice is composited is as culture medium, ferment respectively, make living bacteria count>=5 × 10
8the feed preblending agent of CFU/ml.
In some embodiments of the invention, comprise the following steps:
1) purslane, hairyvein agrimony, the Radix Astragali, wolf's milk and Radix Glycyrrhizae are put in water according to the weight ratio of 1 ~ 2:1 ~ 2:1 ~ 2:1 ~ 2:1 ~ 2, obtain Chinese medicine extract through boiling;
2) carbon source, nitrogenous source, inorganic salts, trace element are joined in described Chinese medicine extract, after sterilizing, cooling, access described Lactobacillus plantarum, bacillus amyloliquefaciens, bacillus subtilis and hydrogenlike silicon ion respectively, ferment respectively, respectively obtained Lactobacillus plantarum living bacterial liquid, bacillus amyloliquefaciens living bacterial liquid, bacillus subtilis living bacterial liquid and hydrogenlike silicon ion living bacterial liquid;
3) described four kinds of living bacterial liquids and bread microzyme living bacterial liquid are joined in mixed carrier, obtained described feed preblending agent after fermentation.
In some embodiments of the invention, the preparation method of described Lactobacillus plantarum living bacterial liquid comprises:
Glucose, water soluble starch, dusty yeast, whey powder, ammonium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium chloride, NaOH, thiamine, niacinamide, taurine, Chinese medicine extract are mixed with water; regulate pH to 6.5 ~ 7.5; Lactobacillus plantarum is inoculated after sterilizing, cooling; in 32 ~ 35 DEG C of condition bottom fermentations 20 ~ 40 hours, obtain containing viable bacteria amount>=3 × 10
8the Lactobacillus plantarum living bacterial liquid of CFU/ml.
In some embodiments of the invention, the preparation method of described bacillus amyloliquefaciens living bacterial liquid comprises:
Glucose, corn flour, water soluble starch, dusty yeast, ammonium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium chloride, sodium chloride, pyridoxine, niacinamide, manganese sulfate, Chinese medicine extract are mixed with water, regulate pH to 7.0 ~ 9.0, bacillus amyloliquefaciens is inoculated after sterilizing, cooling, in 28 ~ 30 DEG C of condition bottom fermentations 15 ~ 35 hours, obtain containing viable bacteria amount>=1 × 10
9the bacillus amyloliquefaciens living bacterial liquid of CFU/ml.
In some embodiments of the invention, the preparation method of described bacillus subtilis living bacterial liquid comprises:
Glucose, corn flour, water soluble starch, dusty yeast, ammonium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium chloride, sodium chloride, pyridoxine, niacinamide, manganese sulfate, Chinese medicine extract are mixed with water, regulate pH to 7.5 ~ 8.5, bacillus subtilis is inoculated after sterilizing, cooling, in 28 ~ 35 DEG C of condition bottom fermentations 15 ~ 35 hours, obtain containing viable bacteria amount>=1 × 10
9the bacillus subtilis living bacterial liquid of CFU/ml.
In some embodiments of the invention, the preparation method of described hydrogenlike silicon ion living bacterial liquid comprises:
Ammonium sulfate, edible lactic acid, NaOH, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, magnesium sulfate, manganese sulfate, zinc sulfate, ferrous sulfate, copper sulphate, p-aminobenzoic acid, niacinamide, pyridoxine, Chinese medicine extract mix with water, regulate pH to 6.0 ~ 7.5, hydrogenlike silicon ion is inoculated after sterilizing, cooling, in 28 ~ 35 DEG C of condition bottom fermentations 15 ~ 35 hours, obtain containing viable bacteria amount>=5 × 10
8the hydrogenlike silicon ion living bacterial liquid of CFU/ml.
In some embodiments of the invention, described mixed carrier comprises: 800 ~ 1200 parts of beta glucans, 70 ~ 120 parts of dried silkworm chrysalis meals, 80 ~ 130 parts of fish meal, 5 ~ 20 parts of dusty yeasts, 50 ~ 150 parts of amino acid, 50 ~ 150 portions of corn flour, 20 ~ 80 parts of bean cake powders, 250 ~ 550 parts of rice brans, 50 ~ 120 parts of calcium dihydrogen phosphates, 20 ~ 80 parts of magnesium sulfate, 5 ~ 40 parts of sodium chloride, 2000 ~ 4000 betaines, 700 ~ 1400 parts of xylo-oligosaccharides, 500 ~ 1200 parts of taurines, 30 ~ 80 parts of thiamines, 30 ~ 80 parts of pyridoxines and 100 ~ 300 parts of niacinamide.
The present invention also provides a kind of feed preblending agent, and described feed preblending agent prepares according to the preparation method of above-mentioned feed preblending agent.
As can be seen here, feed preblending agent provided by the invention contains the Chinese traditional medicine composition compositions such as abundant active polysaccharide, flavones and viable bacteria body and metabolite thereof, it can be used as the micro-ecological feed pre-mixing agent of prawn culturing, viable bacteria body and metabolism lactic acid thereof can suppress enteropathogenic bacteria growing, active traditional Chinese medicine ingredients, bacterial metabolism product (amino acid, vitamin, trace element etc.) are more easily absorbed by shrimp, can play and reduce disease, the reduction death rate, the multiple-effect of growth promoting effects, increase yield.
Detailed description of the invention
For making the object, technical solutions and advantages of the present invention clearly understand, below in conjunction with specific embodiment, the present invention is described in more detail.
Fermented bacterium:
Lactobacillus plantarum (Lactobacillusplantarum) BW2013 bacterial strain, China Committee for Culture Collection of Microorganisms's common micro-organisms center is deposited on September 5th, 2014, deposit number is: CGMCCNo.9642, depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101.
Bacillus amyloliquefaciens (Bacillusamyloliquefaciens) A3 bacterial strain, China Committee for Culture Collection of Microorganisms's common micro-organisms center is deposited on October 15th, 2012, deposit number is: preservation enters an accession designation number CGMCCNO.6676, depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101.
Bacillus subtilis (Bacillussubtilis) szx11 bacterial strain, China Committee for Culture Collection of Microorganisms's common micro-organisms center is deposited on September 16th, 2013, deposit number is: CGMCCNO.8188, depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101.
Hydrogenlike silicon ion (Rhodobactersphaeroides) WD-2 bacterial strain, China Committee for Culture Collection of Microorganisms's common micro-organisms center is deposited on April 20th, 2010, deposit number is: CGMCCNO.3757, depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101.
Bread microzyme, market is bought.
Embodiment 1
As one embodiment of the present of invention, the preparation method of described feed preblending agent comprises the following steps:
1) Chinese medicine extract is prepared:
Put in 1000L Chinese medicine extracting tank by purslane 10kg, hairyvein agrimony 10kg, Radix Astragali 10kg, wolf's milk 10kg, Radix Glycyrrhizae 10kg, add water 500L wherein, is warming up to 100 DEG C, then filters after boiling 1h, obtain filtrate after soaking 1h; Add water 300L again in filter residue, and 100 DEG C of boilings were filtered after 40 minutes, merged twice filtrate, obtained Chinese medicine extract.By Chinese medicine extract stored in holding vessel, for subsequent use.
2) four kinds of living bacterial liquids are prepared respectively:
The preparation method following (for 1000L) of Lactobacillus plantarum living bacterial liquid: by glucose 6kg, water soluble starch 10kg, dusty yeast 4kg, whey powder 4kg, ammonium sulfate 3kg, potassium dihydrogen phosphate 350g, dipotassium hydrogen phosphate 250g, calcium chloride 150g, NaOH 500g, thiamine 6g, niacinamide 20g, taurine 10g, Chinese medicine extract 500L and water 500L mixes, stir, pH value of solution to 7.0 is regulated with sodium hydroxide solution and lactic acid, then in 120 DEG C of sterilizings, be cooled to 35 DEG C of inoculations again, inoculum concentration 10%, control temperature 32 ~ 35 DEG C fermentation 30h, obtain containing viable bacteria amount>=3 × 10
8the Lactobacillus plantarum living bacterial liquid of CFU/ml.
The preparation method following (for 1000L) of bacillus amyloliquefaciens living bacterial liquid: by glucose 3kg, corn flour 10kg, water soluble starch 10kg, dusty yeast 6kg, ammonium sulfate 4kg, potassium dihydrogen phosphate 400g, dipotassium hydrogen phosphate 250g, calcium chloride 1.2kg, sodium chloride 1.0kg, pyridoxine 10g, niacinamide 20g, manganese sulfate 1.5g, Chinese medicine extract 500L and water 500L mixes, stir, regulate pH value of solution to 8.0, then in 120 DEG C of sterilizings, be cooled to 32 DEG C of inoculations again, inoculum concentration 10%, control temperature 30 ~ 32 DEG C, 160r/min stirs fermentation 24h, obtain containing viable bacteria amount>=1 × 10
9the bacillus amyloliquefaciens living bacterial liquid of CFU/ml.
The preparation method following (for 1000L) of bacillus subtilis living bacterial liquid: by glucose 3kg, corn flour 10kg, water soluble starch 10kg, dusty yeast 6kg, ammonium sulfate 4kg, potassium dihydrogen phosphate 400g, dipotassium hydrogen phosphate 250g, calcium chloride 1.2kg, sodium chloride 1.0kg, pyridoxine 10g, niacinamide 20g, manganese sulfate 1.5g, Chinese medicine extract 500L and water 500L, regulate pH value of solution to 8.0, then in 120 DEG C of sterilizings, be cooled to 32 DEG C of inoculations again, inoculum concentration 10%, control temperature 30 ~ 32 DEG C, 160r/min stirs fermentation 24h, obtain containing viable bacteria amount>=1 × 10
9the bacillus subtilis living bacterial liquid of CFU/ml.
The preparation method following (for 1000L) of hydrogenlike silicon ion living bacterial liquid: by ammonium sulfate 2600g, edible lactic acid 6.5L, NaOH 1600g, potassium dihydrogen phosphate 650g, dipotassium hydrogen phosphate 350g, magnesium sulfate 180g, manganese sulfate 1.0g, zinc sulfate 1.2g, ferrous sulfate 5g, copper sulphate 40mg, p-aminobenzoic acid 250mg, niacinamide 2g, pyridoxine 1.0g, Chinese medicine extract 300L, add water to 1000L again, regulate pH value of solution to 6.6 ~ 6.8, then in 121 DEG C of sterilizings, be cooled to 32 DEG C of inoculations again, inoculum concentration 10%, control temperature 30 ~ 32 DEG C, pass into compression to purify air, adjustment venting pressure 0.2Mpa gas-lifting type stirs fermentation 24h, obtain containing viable bacteria amount>=5 × 10
8the hydrogenlike silicon ion living bacterial liquid of CFU/ml.
The preparation method following (for 100L) of bread microzyme living bacterial liquid: 100L water is dropped in actication of culture tank, water is heated to 100 DEG C of sterilizings, keep 30 minutes, be cooled to 32 ~ 35 DEG C, again bread viable yeast bacterial classification 2kg is put in water, 120r/min stirs 30min, obtains viable bacteria content>=1 × 10
8bread microzyme living bacterial liquid.
3) beta glucan 1000g, dried silkworm chrysalis meal 90kg, fish meal 100kg, dusty yeast 10kg, amino acid/11 00kg, corn flour 100kg, bean cake powder 50kg, rice bran 400kg, calcium dihydrogen phosphate 80kg, magnesium sulfate 50kg, sodium chloride 20kg, betaine 3000g, xylo-oligosaccharide 1000g, taurine 800g, thiamine 50g, pyridoxine 50g and niacinamide 200g are mixed, obtained mixed carrier.
Get mixed carrier 1000kg, Lactobacillus plantarum living bacterial liquid 50kg, bacillus amyloliquefaciens living bacterial liquid 20kg, bacillus subtilis living bacterial liquid 20kg, hydrogenlike silicon ion living bacterial liquid 100kg, bread microzyme living bacterial liquid 10kg.
Mixed carrier is proceeded in dry powder auger tank, pass into steam by tank body chuck while stirring and heat to 105 DEG C of maintenance 1h sterilizing, be then cooled to 32 ~ 35 DEG C.Joined while stirring in mixed carrier by above-mentioned five kinds of living bacterial liquids 100,000 grades of cleaning shops, adjustment temperature to 30 ~ 35 DEG C, after auger ferments 4 hours, make effective viable bacteria and are about 9 × 10
9the Penaeus Vannmei fermentation bait pre-mixing agent of cfu/g.
Embodiment 2
As an alternative embodiment of the invention, the preparation method of described feed preblending agent comprises the following steps:
1) Chinese medicine extract is prepared:
Put in 1000L Chinese medicine extracting tank by purslane 15kg, hairyvein agrimony 10kg, Radix Astragali 20kg, wolf's milk 13kg, Radix Glycyrrhizae 18kg, add water 550L wherein, is warming up to 100 DEG C, then filters after boiling 1.2h, obtain filtrate after soaking 1.2h; Add water 280L again in filter residue, and 100 DEG C of boilings were filtered after 35 minutes, merged twice filtrate, obtained Chinese medicine extract.By Chinese medicine extract stored in holding vessel, for subsequent use.
2) four kinds of living bacterial liquids are prepared respectively:
The preparation method following (for 1000L) of Lactobacillus plantarum living bacterial liquid: by glucose 6.5kg, water soluble starch 8kg, dusty yeast 4.5kg, whey powder 4.2kg, ammonium sulfate 2.7kg, potassium dihydrogen phosphate 360g, dipotassium hydrogen phosphate 235g, calcium chloride 155g, NaOH 520g, thiamine 6.5g, niacinamide 22g, taurine 12g, Chinese medicine extract 450L and water 550L mixes, stir, pH value of solution to 7.2 is regulated with sodium hydroxide solution and lactic acid, then in 110 DEG C of sterilizings, be cooled to 35 DEG C of inoculations again, inoculum concentration 12%, control temperature 32 ~ 35 DEG C fermentation 40h, obtain containing viable bacteria amount>=3 × 10
8the Lactobacillus plantarum living bacterial liquid of CFU/ml.
The preparation method following (for 1000L) of bacillus amyloliquefaciens living bacterial liquid: by glucose 2.5kg, corn flour 13kg, water soluble starch 10kg, dusty yeast 5.5kg, ammonium sulfate 4.5kg, potassium dihydrogen phosphate 450g, dipotassium hydrogen phosphate 200g, calcium chloride 1.0kg, sodium chloride 1.2kg, pyridoxine 10g, niacinamide 25g, manganese sulfate 1.2g, Chinese medicine extract 480L and water 520L mixes, stir, regulate pH value of solution to 8.2, then in 110 DEG C of sterilizings, be cooled to 32 DEG C of inoculations again, inoculum concentration 11%, control temperature 30 ~ 32 DEG C, 180r/min stirs fermentation 30h, obtain containing viable bacteria amount>=1 × 10
9the bacillus amyloliquefaciens living bacterial liquid of CFU/ml.
The preparation method following (for 1000L) of hydrogenlike silicon ion living bacterial liquid: by ammonium sulfate 2800g, edible lactic acid 6.0L, NaOH 1500g, potassium dihydrogen phosphate 680g, dipotassium hydrogen phosphate 360g, magnesium sulfate 160g, manganese sulfate 1.2g, zinc sulfate 1.0g, ferrous sulfate 5.6g, copper sulphate 42mg, p-aminobenzoic acid 240mg, niacinamide 2.2g, pyridoxine 1.1g, Chinese medicine extract 400L, add water to 1000L again, regulate pH value of solution to 6.0 ~ 6.5, then in 112 DEG C of sterilizings, be cooled to 32 DEG C of inoculations again, inoculum concentration 13%, control temperature 30 ~ 32 DEG C, pass into compression to purify air, adjustment venting pressure 0.2Mpa gas-lifting type stirs fermentation 28h, obtain containing viable bacteria amount>=5 × 10
8the hydrogenlike silicon ion living bacterial liquid of CFU/ml.
The preparation method following (for 100L) of bread microzyme living bacterial liquid: 100L water is dropped in actication of culture tank, water is heated to 100 DEG C of sterilizings, keep 35 minutes, be cooled to 30 ~ 33 DEG C, again bread viable yeast bacterial classification 1.8kg is put in water, 130r/min stirs 30min, obtains viable bacteria content>=1 × 10
8bread microzyme living bacterial liquid.
3) beta glucan 950g, dried silkworm chrysalis meal 100kg, fish meal 110kg, dusty yeast 15kg, amino acid/11 20kg, corn flour 95kg, bean cake powder 45kg, rice bran 420kg, calcium dihydrogen phosphate 70kg, magnesium sulfate 40kg, sodium chloride 30kg, betaine 3050g, xylo-oligosaccharide 1100g, taurine 1000g, thiamine 55g, pyridoxine 55g and niacinamide 250g are mixed, obtained mixed carrier.
Get mixed carrier 1000kg, Lactobacillus plantarum living bacterial liquid 50kg, bacillus amyloliquefaciens living bacterial liquid 25kg, bacillus subtilis living bacterial liquid 20kg, hydrogenlike silicon ion living bacterial liquid 120kg, bread microzyme living bacterial liquid 8kg.
Mixed carrier is proceeded in dry powder auger tank, pass into steam by tank body chuck while stirring and heat to 110 DEG C of maintenance 1.2h sterilizing, be then cooled to 32 ~ 35 DEG C.Joined while stirring in mixed carrier by above-mentioned five kinds of living bacterial liquids 100,000 grades of cleaning shops, adjustment temperature to 33 ~ 35 DEG C, after auger ferments 5 hours, make effective viable bacteria and are about 1 × 10
10the Penaeus Vannmei fermentation bait pre-mixing agent of cfu/g.
Embodiment 3
As an alternative embodiment of the invention, the preparation method of described feed preblending agent comprises the following steps:
1) Chinese medicine extract is prepared:
Put in 1000L Chinese medicine extracting tank by purslane 10kg, hairyvein agrimony 20kg, Radix Astragali 12kg, wolf's milk 16kg, Radix Glycyrrhizae 15kg, add water 600L wherein, is warming up to 100 DEG C, then filters after boiling 1.5h, obtain filtrate after soaking 0.8h; Add water 320L again in filter residue, and 100 DEG C of boilings were filtered after 45 minutes, merged twice filtrate, obtained Chinese medicine extract.By Chinese medicine extract stored in holding vessel, for subsequent use.
2) four kinds of living bacterial liquids are prepared respectively:
The preparation method following (for 1000L) of Lactobacillus plantarum living bacterial liquid: by glucose 5.6kg, water soluble starch 10.5kg, dusty yeast 4.2kg, whey powder 4kg, ammonium sulfate 3.3kg, potassium dihydrogen phosphate 375g, dipotassium hydrogen phosphate 260g, calcium chloride 145g, NaOH 490g, thiamine 5.4g, niacinamide 19g, taurine 10g, Chinese medicine extract 550L and water 450L mixes, stir, pH value of solution to 6.8 is regulated with sodium hydroxide solution and lactic acid, then in 115 DEG C of sterilizings, be cooled to 35 DEG C of inoculations again, inoculum concentration 8%, control temperature 32 ~ 35 DEG C fermentation 28h, obtain containing viable bacteria amount>=3 × 10
8the Lactobacillus plantarum living bacterial liquid of CFU/ml.
The preparation method following (for 1000L) of bacillus amyloliquefaciens living bacterial liquid: by glucose 3.4kg, corn flour 10kg, water soluble starch 14kg, dusty yeast 5.8kg, ammonium sulfate 4.4kg, potassium dihydrogen phosphate 360g, dipotassium hydrogen phosphate 260g, calcium chloride 1.5kg, sodium chloride 0.8kg, pyridoxine 12g, niacinamide 25g, manganese sulfate 1.25g, Chinese medicine extract 460L and water 640L mixes, stir, regulate pH value of solution to 7.8, then in 115 DEG C of sterilizings, be cooled to 32 DEG C of inoculations again, inoculum concentration 9%, control temperature 30 ~ 32 DEG C, 150r/min stirs fermentation 20h, obtain containing viable bacteria amount>=1 × 10
9the bacillus amyloliquefaciens living bacterial liquid of CFU/ml.
The preparation method following (for 1000L) of hydrogenlike silicon ion living bacterial liquid: by ammonium sulfate 2500g, edible lactic acid 5.5L, NaOH 1650g, potassium dihydrogen phosphate 600g, dipotassium hydrogen phosphate 400g, magnesium sulfate 200g, manganese sulfate 0.88g, zinc sulfate 1.2g, ferrous sulfate 6g, copper sulphate 45mg, p-aminobenzoic acid 270mg, niacinamide 2.5g, pyridoxine 0.85g, Chinese medicine extract 350L, add water to 1000L again, regulate pH value of solution to 6.9 ~ 7.3, then in 115 DEG C of sterilizings, be cooled to 32 DEG C of inoculations again, inoculum concentration 8%, control temperature 28 ~ 30 DEG C, pass into compression to purify air, adjustment venting pressure 0.25Mpa gas-lifting type stirs fermentation 20h, obtain containing viable bacteria amount>=5 × 10
8the hydrogenlike silicon ion living bacterial liquid of CFU/ml.
The preparation method following (for 100L) of bread microzyme living bacterial liquid: 100L water is dropped in actication of culture tank, water is heated to 100 DEG C of sterilizings, keep 25 minutes, be cooled to 33 ~ 35 DEG C, again bread viable yeast bacterial classification 2.2kg is put in water, 120r/min stirs 40min, obtains viable bacteria content>=1 × 10
8bread microzyme living bacterial liquid.
3) beta glucan 1100g, dried silkworm chrysalis meal 100kg, fish meal 100kg, dusty yeast 12kg, amino acid 95kg, corn flour 105kg, bean cake powder 60kg, rice bran 380kg, calcium dihydrogen phosphate 88kg, magnesium sulfate 46kg, sodium chloride 23kg, betaine 2950g, xylo-oligosaccharide 980g, taurine 770g, thiamine 57g, pyridoxine 51g and niacinamide 190g are mixed, obtained mixed carrier.
Get mixed carrier 1000kg, Lactobacillus plantarum living bacterial liquid 60kg, bacillus amyloliquefaciens living bacterial liquid 20kg, bacillus subtilis living bacterial liquid 24kg, hydrogenlike silicon ion living bacterial liquid 92kg, bread microzyme living bacterial liquid 12kg.
Mixed carrier is proceeded in dry powder auger tank, pass into steam by tank body chuck while stirring and heat to 108 DEG C of maintenance 1h sterilizing, be then cooled to 32 ~ 34 DEG C.Joined while stirring in mixed carrier by above-mentioned five kinds of living bacterial liquids 100,000 grades of cleaning shops, adjustment temperature to 30 ~ 34 DEG C, after auger ferments 3.5 hours, make effective viable bacteria and are about 8 × 10
8the Penaeus Vannmei fermentation bait pre-mixing agent of cfu/g.
Embodiment 4
As an alternative embodiment of the invention, the preparation method of described feed preblending agent comprises the following steps:
1) Chinese medicine extract is prepared:
Put in 1000L Chinese medicine extracting tank by purslane 10kg, hairyvein agrimony 12kg, Radix Astragali 10kg, wolf's milk 18kg, Radix Glycyrrhizae 10kg, add water 580L wherein, is warming up to 100 DEG C, then filters after boiling 1.5h, obtain filtrate after soaking 1h; Add water 330L again in filter residue, and 100 DEG C of boilings were filtered after 38 minutes, merged twice filtrate, obtained Chinese medicine extract.By Chinese medicine extract stored in holding vessel, for subsequent use.
2) four kinds of living bacterial liquids are prepared respectively:
The preparation method following (for 1000L) of Lactobacillus plantarum living bacterial liquid: by glucose 5kg, water soluble starch 10kg, dusty yeast 5kg, whey powder 5kg, ammonium sulfate 3.5kg, potassium dihydrogen phosphate 300g, dipotassium hydrogen phosphate 300g, calcium chloride 170g, NaOH 460g, thiamine 6.8g, niacinamide 25g, taurine 6g, Chinese medicine extract 600L and water 400L mixes, stir, pH value of solution to 7.0 is regulated with sodium hydroxide solution and lactic acid, then in 120 DEG C of sterilizings, be cooled to 35 DEG C of inoculations again, inoculum concentration 10%, control temperature 32 ~ 35 DEG C fermentation 30h, obtain containing viable bacteria amount>=3 × 10
8the Lactobacillus plantarum living bacterial liquid of CFU/ml.
The preparation method following (for 1000L) of bacillus amyloliquefaciens living bacterial liquid: by glucose 3.5kg, corn flour 9.3kg, water soluble starch 8.8kg, dusty yeast 8kg, ammonium sulfate 5kg, potassium dihydrogen phosphate 380g, dipotassium hydrogen phosphate 280g, calcium chloride 1.3kg, sodium chloride 1.2kg, pyridoxine 12g, niacinamide 16g, manganese sulfate 1.6g, Chinese medicine extract 500L and water 500L mixes, stir, regulate pH value of solution to 8.5, then in 112 DEG C of sterilizings, be cooled to 32 DEG C of inoculations again, inoculum concentration 12%, control temperature 30 ~ 32 DEG C, 170r/min stirs fermentation 28h, obtain containing viable bacteria amount>=1 × 10
9the bacillus amyloliquefaciens living bacterial liquid of CFU/ml.
The preparation method following (for 1000L) of hydrogenlike silicon ion living bacterial liquid: by ammonium sulfate 2680g, edible lactic acid 5.5L, NaOH 1550g, potassium dihydrogen phosphate 700g, dipotassium hydrogen phosphate 390g, magnesium sulfate 188g, manganese sulfate 1.3g, zinc sulfate 1.0g, ferrous sulfate 5.5g, copper sulphate 50mg, p-aminobenzoic acid 260mg, niacinamide 2.2g, pyridoxine 1.1g, Chinese medicine extract 600L, add water to 1000L again, regulate pH value of solution to 7.1 ~ 7.5, then in 110 DEG C of sterilizings, be cooled to 32 DEG C of inoculations again, inoculum concentration 10%, control temperature 30 ~ 35 DEG C, pass into compression to purify air, adjustment venting pressure 0.15Mpa gas-lifting type stirs fermentation 20h, obtain containing viable bacteria amount>=5 × 10
8the hydrogenlike silicon ion living bacterial liquid of CFU/ml.
The preparation method following (for 100L) of bread microzyme living bacterial liquid: 100L water is dropped in actication of culture tank, water is heated to 100 DEG C of sterilizings, keep 30 minutes, be cooled to 32 ~ 35 DEG C, again bread viable yeast bacterial classification 2kg is put in water, 120r/min stirs 30min, obtains viable bacteria content>=1 × 10
8bread microzyme living bacterial liquid.
3) beta glucan 980g, dried silkworm chrysalis meal 98kg, fish meal 80kg, dusty yeast 12kg, amino acid 98kg, corn flour 90kg, bean cake powder 53kg, rice bran 400kg, calcium dihydrogen phosphate 80kg, magnesium sulfate 44kg, sodium chloride 28kg, betaine 3100g, xylo-oligosaccharide 985g, taurine 800g, thiamine 52g, pyridoxine 51g and niacinamide 210g are mixed, obtained mixed carrier.
Get mixed carrier 1000kg, Lactobacillus plantarum living bacterial liquid 60kg, bacillus amyloliquefaciens living bacterial liquid 20kg, bacillus subtilis living bacterial liquid 27kg, hydrogenlike silicon ion living bacterial liquid 90kg, bread microzyme living bacterial liquid 15kg.
Mixed carrier is proceeded in dry powder auger tank, pass into steam by tank body chuck while stirring and heat to 102 DEG C of maintenance 2h sterilizing, be then cooled to 34 ~ 35 DEG C.Joined while stirring in mixed carrier by above-mentioned five kinds of living bacterial liquids 100,000 grades of cleaning shops, adjustment temperature to 30 ~ 32 DEG C, after auger ferments 5.5 hours, make effective viable bacteria and are about 1.5 × 10
10the Penaeus Vannmei fermentation bait pre-mixing agent of cfu/g.
The purposes of feed preblending agent described in embodiment 5
The purposes of described feed preblending agent comprises:
(1) described fermentation bait pre-mixing agent is admixed in feed according to the 10%-20% of feeding quality, and before throwing something and feeding 0.5 ~ 2 hour premix, then the warm water admixing feed gross weight 10 ~ 20%, to strengthen humidity, is convenient to the beneficial flora activation breeding in feed preblending agent.
(2) 0.5 ~ 2 hour is shifted to an earlier date in clean container or polybag by this feed preblending agent and feed premix, and cover tightly container or tighten plastic bag mouth bait pre-mixing agent beneficial flora is fully activated, and the Effective Component of Chinese Medicine in fermentation bait pre-mixing agent and activation thalline are fully attached in feed.Need to add described fermentation bait pre-mixing agent every day 1-2 time according to cultivation.
(3), when throwing something and feeding, the feed mixing mixed fermentation bait pre-mixing agent is directly rendered on the material platform on the shrimp pool.
The nursing experiment of feed preblending agent described in embodiment 6
(1) Penaeus Vannmei feeds experiment in 99 days
Place: lake-light shrimp field, Chikan District, Zhanjiang, Guangdong Province, feed coefficient intensive culture.
Experimental technique: adopt with batch shrimp seedling, throw on the same day.Select honest feed, setting experiment 1 by 1.5%, experiment 2 by 2.0%, experiment 3 mixes by 2.5% and feeds feed preblending agent provided by the invention, every day sooner or later mixes and feeds twice.The warm water of control group premix isodose.Dead shrimp counting is collected the dirty pipe box net in every day from the row end.
26 DEG C of times of average salinity 15 ‰ average temperature of breeding water body: 9 days-August 17 May in 2015
| Subject | Area (mu) | Throw seedling (tail/mu) | 30 days average substances | 92 days substances | The death rate | Average yield per mu |
| Experiment 1 | 4.2 | 60000 | 6.12g | 26.3g | 11.50％ | 1396kg |
| Experiment 2 | 4.0 | 60000 | 6.12g | 24.4g | 13.0％ | 1244kg |
| Experiment 3 | 4.0 | 60000 | 6.16g | 24.0g | 14.8％ | 1226kg |
| Contrast 1 | 3.5 | 60000 | 4.8g | 17.95g | 45.8％ | 583kg |
| Contrast 2 | 4.0 | 60000 | 5.0g | 19.0g | 48.4％ | 668kg |
| Contrast 3 | 4.0 | 60000 | 4.5g | 18.0g | 50.26％ | 537kg |
(2) Penaeus Vannmei feeds experiment in 99 days
Place: symbol shrimp field, Qionghai mayor slope town, Hainan Province, high-order culture pond.Experimental technique: adopt with batch Zhengda Company's shrimp seedling, select Zhengda Company's feed.Setting experiment 1 by 1.5%, experiment 2 by 2.0%, experiment 3 mixes by 2.5% and feeds feed preblending agent provided by the invention, every day sooner or later mixes and feeds twice.Control group mixes the warm water of feeding isodose.Dead shrimp counting is collected the dirty pipe box net in every day from the row end.
Pure 26.2 DEG C of times of freshwater aquiculture water body average temperature: 23 days-July 1 March in 2015
| Subject | Area (mu) | Throw seedling (tail/mu) | 50 days average substances | 120 days substances | The death rate | Average yield per mu |
| Experiment 1 | 2.8 | 50000 | 9.10g | 28.05g | 18.25％ | 1146kg |
| Experiment 2 | 2.9 | 50000 | 8.16g | 27.80g | 23.45％ | 1064kg |
| Experiment 3 | 3.3 | 50000 | 8.11g | 29.0g | 24.43％ | 1096kg |
| Contrast 1 | 3.1 | 50000 | 7.33g | 24.4g | 36.0％ | 784kg |
| Contrast 2 | 2.6 | 50000 | 7.89g | 22.7g | 40.50％ | 675kg |
| Contrast 3 | 2.8 | 50000 | 7.75g | 22.7g | 45.80％ | 722kg |
(3) Penaeus Vannmei feeds experiment in 99 days
Place: East, Leizhou City, Guangdong Province Zhang Mei plant (the high-order pond of warmhouse booth).
Experimental technique: adopt with batch positive prawn seedling, select Guangdong Yue Hai feed, setting experiment 1 by 1.5%, experiment 2 by 2.0%, experiment 3 mixes by 2.5% and feeds feed preblending agent provided by the invention, every day sooner or later mixes and feeds twice.Control group mixes the warm water of feeding isodose.Dead shrimp counting is collected the dirty pipe box net in every day from the row end.
The average salinity of breeding water body is 12 days February-2015 years on the 4th November in 2014 of 20 ‰ average temperature 24.2 DEG C
| Subject | Area (mu) | Throw seedling (tail/mu) | 30 days average substances | 109 days substances | The death rate | Average yield per mu |
| Experiment 1 | 2.6 | 80000 | 4.97g | 21.9g | 14.0％ | 1425kg |
| Experiment 2 | 2.4 | 80000 | 5.13g | 20.8g | 13.6％ | 1370kg |
| Experiment 3 | 2.5 | 80000 | 5.10g | 20.5g | 15.5％ | 1296kg |
| Experiment 4 | 2.9 | 80000 | 5.08g | 19.g | 12.8％ | 1234kg |
| Contrast 1 | 3.1 | 80000 | 3.69g | 17.5g | 48.0％ | 728kg |
| Contrast 2 | 2.2 | 80000 | 4.66g | 18.2g | 45.0％ | 800kg |
| Contrast 3 | 2.2 | 80000 | 4.69g | 19.2g | 41.5％ | 887kg |
(4) Penaeus Vannmei feeds experiment in 99 days
Place: Chen Gui prawn field, sea-gull island, Fanyu District, Guangdong Province, Low-salinity Tu Tang half intensive culture.
Experimental technique: adopt with batch shrimp seedling, throw on the same day.Select honest feed, setting experiment 1 by 1.5%, experiment 2 by 2.0%, experiment 3 mixes by 2.5% and feeds feed preblending agent provided by the invention, every day sooner or later mixes and feeds twice.The warm water of control group premix isodose.Dead shrimp counting is collected the dirty pipe box net in every day from the row end.
28.2 DEG C of times of breeding water body average salinity 1.0/1000 average temperature: 5 days-July 13 April in 2014
| Area (mu) | Throw seedling (tail/mu) | 40 days average substances | 90 days substances | The death rate | Average yield per mu | |
| Experiment 1 | 3.3 | 50000 | 5.80g | 12.46g | 18.65％ | 505kg |
| Experiment 2 | 3.1 | 50000 | 6.32g | 15.06g | 17.00％ | 625kg |
| Experiment 3 | 2.4 | 50000 | 6.12g | 14.89g | 18.87％ | 605kg |
| Contrast 1 | 3.0 | 50000 | 4.21g | 10.56g | 54.90％ | 290kg |
| Contrast 2 | 3.8 | 50000 | 4.37g | 10.61g | 48.18％ | 275kg |
| Contrast 3 | 3.8 | 50000 | 4.05g | 10.61g | 50.15％ | 282kg |
Feed preblending agent provided by the invention contains the Chinese traditional medicine composition compositions such as abundant active polysaccharide, flavones and probio and metabolite thereof, it can be used as the micro-ecological feed pre-mixing agent of prawn culturing, there is good prevention effect to Penaeus Vannmei enteron aisle disease, obviously can reduce the empty stomach of its jejunum, enteritis, the white enteron aisle disease such as just; Significantly improve cultivation survival rate.Obtain zymotic fluid through method provided by the invention, as the feed preblending agent Application and Development of culture of Penaeus vannamei, there are wide market prospects.
Feed preblending agent provided by the invention is the fermented feed pre-mixing agent having feed nutrition, combined hearth-care bacterium, traditional Chinese medicine ingredients concurrently; be different from feed addictive; play a major role is protection prawn liver pancreas, raising immunologic function; the early stage comprehensive dead disease of prevention prawn; the output of prawn can be significantly improved, reduce the death rate.
Those of ordinary skill in the field are to be understood that: the discussion of above any embodiment is only exemplary, and not intended to be implies that the scope of the present disclosure (comprising claim) is limited to these examples; Under thinking of the present invention, can combine between the technical characteristic in above embodiment or different embodiment yet, and there are other changes many of different aspect of the present invention as above, they do not provide in details for the sake of simplicity.Therefore, within the spirit and principles in the present invention all, any omission made, amendment, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. the preparation method of a feed preblending agent, it is characterized in that, comprise: with Lactobacillus plantarum LactobacillusplantarumBW2013 bacterial strain, bacillus amyloliquefaciens BacillusamyloliquefaciensA3 bacterial strain, Bacillus subtilis szx11 bacterial strain, hydrogenlike silicon ion RhodobactersphaeroidesWD-2 bacterial strain and bread microzyme are fermented bacterium, with purslane extract, Herba Agrimoniae extract, Astragalus Root P.E, Herba Euphorbiae Humifusae extract, the complex culture medium that licorice is composited is as culture medium, ferment respectively, make living bacteria count>=5 × 10
8the feed preblending agent of CFU/ml.
2. the preparation method of feed preblending agent according to claim 1, is characterized in that, comprises the following steps:
1) purslane, hairyvein agrimony, the Radix Astragali, wolf's milk and Radix Glycyrrhizae are put in water according to the weight ratio of 1 ~ 2:1 ~ 2:1 ~ 2:1 ~ 2:1 ~ 2, obtain Chinese medicine extract through boiling;
2) carbon source, nitrogenous source, inorganic salts, trace element are joined in described Chinese medicine extract, after sterilizing, cooling, access described Lactobacillus plantarum, bacillus amyloliquefaciens, bacillus subtilis and hydrogenlike silicon ion respectively, ferment respectively, respectively obtained Lactobacillus plantarum living bacterial liquid, bacillus amyloliquefaciens living bacterial liquid, bacillus subtilis living bacterial liquid and hydrogenlike silicon ion living bacterial liquid;
3) described four kinds of living bacterial liquids and bread microzyme living bacterial liquid are joined in mixed carrier, obtained described feed preblending agent after fermentation.
3. the preparation method of feed preblending agent according to claim 2, is characterized in that, the preparation method of described Lactobacillus plantarum living bacterial liquid comprises:
Glucose, water soluble starch, dusty yeast, whey powder, ammonium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium chloride, NaOH, thiamine, niacinamide, taurine, Chinese medicine extract are mixed with water; regulate pH to 6.5 ~ 7.5; Lactobacillus plantarum is inoculated after sterilizing, cooling; in 32 ~ 35 DEG C of condition bottom fermentations 20 ~ 40 hours, obtain containing viable bacteria amount>=3 × 10
8the Lactobacillus plantarum living bacterial liquid of CFU/ml.
4. the preparation method of feed preblending agent according to claim 2, is characterized in that, the preparation method of described bacillus amyloliquefaciens living bacterial liquid comprises:
Glucose, corn flour, water soluble starch, dusty yeast, ammonium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium chloride, sodium chloride, pyridoxine, niacinamide, manganese sulfate, Chinese medicine extract are mixed with water, regulate pH to 7.0 ~ 9.0, bacillus amyloliquefaciens is inoculated after sterilizing, cooling, in 28 ~ 30 DEG C of condition bottom fermentations 15 ~ 35 hours, obtain containing viable bacteria amount>=1 × 10
9the bacillus amyloliquefaciens living bacterial liquid of CFU/ml.
5. the preparation method of feed preblending agent according to claim 2, is characterized in that, the preparation method of described bacillus subtilis living bacterial liquid comprises:
Glucose, corn flour, water soluble starch, dusty yeast, ammonium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium chloride, sodium chloride, pyridoxine, niacinamide, manganese sulfate, Chinese medicine extract are mixed with water, regulate pH to 7.5 ~ 8.5, bacillus subtilis is inoculated after sterilizing, cooling, in 28 ~ 35 DEG C of condition bottom fermentations 15 ~ 35 hours, obtain containing viable bacteria amount>=1 × 10
9the bacillus subtilis living bacterial liquid of CFU/ml.
6. the preparation method of feed preblending agent according to claim 2, is characterized in that, the preparation method of described hydrogenlike silicon ion living bacterial liquid comprises:
Ammonium sulfate, edible lactic acid, NaOH, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, magnesium sulfate, manganese sulfate, zinc sulfate, ferrous sulfate, copper sulphate, p-aminobenzoic acid, niacinamide, pyridoxine, Chinese medicine extract mix with water, regulate pH to 6.0 ~ 7.5, hydrogenlike silicon ion is inoculated after sterilizing, cooling, in 28 ~ 35 DEG C of condition bottom fermentations 15 ~ 35 hours, obtain containing viable bacteria amount>=5 × 10
8the hydrogenlike silicon ion living bacterial liquid of CFU/ml.
7. the preparation method of feed preblending agent according to claim 2, it is characterized in that, described mixed carrier comprises: 800 ~ 1200 parts of beta glucans, 70 ~ 120 parts of dried silkworm chrysalis meals, 80 ~ 130 parts of fish meal, 5 ~ 20 parts of dusty yeasts, 50 ~ 150 parts of amino acid, 50 ~ 150 portions of corn flour, 20 ~ 80 parts of bean cake powders, 250 ~ 550 parts of rice brans, 50 ~ 120 parts of calcium dihydrogen phosphates, 20 ~ 80 parts of magnesium sulfate, 5 ~ 40 parts of sodium chloride, 2000 ~ 4000 betaines, 700 ~ 1400 parts of xylo-oligosaccharides, 500 ~ 1200 parts of taurines, 30 ~ 80 parts of thiamines, 30 ~ 80 parts of pyridoxines and 100 ~ 300 parts of niacinamide.
8. a feed preblending agent, is characterized in that, described feed preblending agent prepares according to the preparation method of the feed preblending agent in claim 1 ~ 7 described in any one.
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| CN107513512A (en) * | 2017-10-16 | 2017-12-26 | 宜宾学院 | The Rhodobacter capsulatus bacterial strain of degrading aspergillus flavus B1 a kind of and its application and degradation agent and its application |
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| CN106417916A (en) * | 2016-09-29 | 2017-02-22 | 张元生 | Licking blocks for ruminants to lick and preparation method thereof |
| CN107513512A (en) * | 2017-10-16 | 2017-12-26 | 宜宾学院 | The Rhodobacter capsulatus bacterial strain of degrading aspergillus flavus B1 a kind of and its application and degradation agent and its application |
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