CN105707453A - Preparing method of feed antibiotic substitute rich in lipopeptid and Chinese herbal medicine effective ingredients - Google Patents
Preparing method of feed antibiotic substitute rich in lipopeptid and Chinese herbal medicine effective ingredients Download PDFInfo
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- CN105707453A CN105707453A CN201610112485.2A CN201610112485A CN105707453A CN 105707453 A CN105707453 A CN 105707453A CN 201610112485 A CN201610112485 A CN 201610112485A CN 105707453 A CN105707453 A CN 105707453A
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Abstract
The invention discloses a preparing method of a feed antibiotic substitute rich in lipopeptid and Chinese herbal medicine effective ingredients. The preparing method comprises the main following steps that Chinese herbal medicine is smashed and screened in a 60-mesh mode for use; the smashed Chinese herbal medicine is added into a fermentation tank, and nutrient and water are added to serve as a fermentation culture medium; after being sterilized and cooled, the fermentation culture medium in the fermentation tank is inoculated with bacillus subtilis HL1 capable of generating anti-bacterial lipopeptid to be subjected to liquid aerobic fermentation; after fermentation is finished, fermentation liquor is subjected to filtration and precipitate removal to obtain supernatant liquor, the supernatant liquor is concentrated in a vacuum mode to obtain a concentrated solution, the concentration solution is adsorbed by an adsorption agent, and an adsorbing system can be prepared into the feed antibiotic substitute after being dried and smashed. The feed antibiotic substitute prepared through the method is rich in lipopeptid and Chinese herbal the medicine effective ingredients, has good properties of promoting growth and resisting disease, can replace feed antibiotic completely in the feed industry, and has a good application prospect.
Description
Technical field
The present invention relates to a kind of rich in lipopeptid with the preparation method of the feeding antibiotic substitute of Chinese herbal medicine effective ingredients, belong to technical field prepared by feed additive.
Background technology
At present the annual about 210,000 tons of antibiotic raw materials produced of China have 9.7 ten thousand tons for livestock breeding industry, account for gross annual output amount 46.1%.The antibiotic that uses of excess not only result in the cultivated animals drug resistance to antibiotic medicine, the resistance against diseases making cultivated animals declines, and these antibiotic can enter human body with food chain, human body is made inadvertently to produce drug-resistant bacteria, the most sick will increasing treats difficulty and expense, it could even be possible to " superbacteria " can be produced, situation about pasting medical help occurs.Along with improving constantly of people's quality of life, varieties of food items is included, and the safe and sanitary of meat product has had higher requirement, the strongest to the cry of " quality-assured meat ", " pollution-free food ".Therefore, the green succedaneum finding antibiotic is problem demanding prompt solution in China's feedstuff and cultivation industry development.
Chinese herbal medicine is the rarity in Chinese medicine treasure-house, and Chinese herbal medicine is as feed additive, has begun in Ancient Times in China.Chinese herbal medicine have have no side effect, be difficult to cause the characteristic such as resistance, noresidue, the green succedaneum as a kind of feeding antibiotic has been widely recognized at feed industry.From the eighties in 20th century, Chinese herbal medicine has been used for making feeding antibiotic succedaneum, and achieves certain application effect.After Chinese herbal medicine undergoes microbial fermentation, its effective ingredient is fully discharged so that it is more biological activity and curative effect study hotspot the most especially.China's feedstuff (2010,419(15): 13-17) document discloses the aspergillus oryzae of isolated from different herbal raw materials and ferments Chinese herbal medicine, and fermentation material cooking liquor the most relatively compares raising 36.03%, 60.83%, 27.64%, 41.32% to the bacteriostatic activity of escherichia coli, staphylococcus aureus, proteus vulgaris and Salmonella typhimurium.But the above fermentation to Chinese herbal medicine is simply confined to fully allow Chinese herbal medicine effective ingredients discharge, thus improves bacteriostatic activity, but the antibacterial activity of Chinese herbal medicine effective ingredients does not promote.Fermentation of Chinese herbal medicine exists maximum problem at present is that using dosage is bigger than normal.This not only adds the use cost of product, but also affect feed nutrition proportioning and palatability, so that fermentation of Chinese herbal medicine is widely used still without at feed industry.Therefore, it is badly in need of increasing substantially the antibacterial activity of effective ingredient in fermentation of Chinese herbal medicine, reduces the fermentation of Chinese herbal medicine using dosage at feed industry, to promote that fermentation of Chinese herbal medicine is in the extensive application of feed industry.
As a kind of green succedaneum of feeding antibiotic, antibacterial peptide is generally of heat-resisting, has a broad antifungal spectrum, good water solubility and is not likely to produce the characteristics such as drug resistance, receiving much concern in recent years.Antibacterial lipopeptid be with bacillus subtilis (Bacillus subtilis), bacillus amyloliquefaciens (Bacillus
amyloliquefaciens), Bacillus natto (Bacillus natto) etc. for representative bacillus cereus produce a series of fat peptide matters.Antibacterial lipopeptid studies have reported that in the application of feed industry in recent years.Patent CN 101974586 A discloses the production method of a kind of bacillus subtilis antibacterial lipopeptid and the application in feedstuff.Bacillus subtilis fmbj through liquid fermentation, concentrate, be spray-dried after make the antibacterial lipopeptid product that a kind of titer is 20000～300000IU/g.By 4000 IU/ kilogram of interpolation antibacterial lipopeptids in daily ration of broiler, improve efficiency of feed utilization, and the growth promoter of broiler and the enhancing of immunity are all had preferable effect.Although antibacterial lipopeptid has a good application prospect at feed industry, but high due to production cost at present, do not obtain actual application at feed industry.Thus, reduce production cost or the using dosage of antibacterial lipopeptid of antibacterial lipopeptid, thus reduced use cost, be to promote that antibacterial lipopeptid solves the technical problem that needed for feed industry is widely applied.
Applicant proposes patent application 201510092596.7 " preparation method of the feed additive of a kind of aquatic product leftovers and Chinese herbal medicine mixed fermentation " on March 2nd, 2015.This patent application discloses with aquatic product leftovers and Chinese herbal medicine as raw material, prepares a kind of rich in Chinese herbal medicine effective ingredients, lipopeptid and the feed additive of little peptide by producing lipopeptid bacillus subtilis solid fermentation.In this patent application, so that the feed additive of preparation is rich in little peptide, add too much aquatic product leftovers in the fermentation medium, carbon-nitrogen ratio serious unbalance in fermentation medium, this had both been unfavorable for the raw lipopeptid of producing bacillus subtilis, also it is unfavorable for that producing bacillus subtilis non-starch polysaccharide enzyme, to Chinese herbal medicine cell wall of degrading, thus greatly reduces the antibacterial activity of the feed additive of preparation.
Summary of the invention
The problem existed for prior art, and the deficiency existing for patent application 201510092596.7 " preparation method of the feed additive of a kind of aquatic product leftovers and Chinese herbal medicine mixed fermentation " that applicant proposes, the patent application 201510092596.7 proposed on March 2nd, 2015 with applicant, as priority, the invention provides a kind of rich in lipopeptid with the preparation method of the feeding antibiotic substitute of Chinese herbal medicine effective ingredients.The main object of the present invention is the production cost of antibacterial lipopeptid to be reduced and Chinese herbal medicine fermentation, but also the antibacterial activity of Chinese herbal medicine effective ingredients to be improved and antibacterial lipopeptid, thus reduce antibacterial lipopeptid and the Chinese herbal medicine using dosage at feed industry, to promote antibacterial lipopeptid and Chinese herbal medicine being widely applied at feed industry.
In order to solve the problems referred to above, the technical solution adopted in the present invention is as follows: a kind of rich in lipopeptid with the preparation method of the feeding antibiotic substitute of Chinese herbal medicine effective ingredients, comprises the steps:
(1) by slant preservation can produce antibacterial lipopeptid bacillus subtilis (Bacillus subtilis) HL-1 is linked into after sterilizing in the LB fluid medium of cooling, in 37 DEG C, 150r/min cultivate 16h, be prepared as bacillus subtilis (Bacillus subtilis) HL-1 seed liquor is standby；
(2) 60 mesh sieves are crossed after being pulverized by Chinese herbal medicine standby；
(3) after step (2) being pulverized, Chinese herbal medicine joins in fermentation tank, and adds water and nutrient in fermentation tank, obtains fermentation medium after mixing；
(4) cultivation in fermentation tank step (3) obtained is based on 121 DEG C of sterilizing 20min；
(5) culture medium in the fermentation tank after sterilizing is cooled to room temperature, then accesses the bacillus subtilis HL-1 seed liquor prepared by step (1)；
(6) control of sweat: fermentation temperature is 30 DEG C～32 DEG C, and filtrated air intake is 0.5vvm～2.5vvm, and the rotating speed of fermentation tank is 150rpm～450rpm, and fermentation time is 35～45h；
(7) product handling system: after fermentation ends, fermentation liquor is crossed and is filtered to remove precipitation, collects supernatant；Supernatant is through being concentrated in vacuo to the 1/3～1/5 of original volume；Then feeding antibiotic substitute finished product can be prepared as after the ratio that concentrated solution and adsorbent are 1～3:1 in mass ratio being carried out mixing and absorption, absorption system drying and pulverizing.
The Bacillus subtillis accessed in described step (1) (Bacillus subtilis) HL-1 can produce antibacterial lipopeptid, this bacterium is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on January 20th, 2015, deposit number is CGMCC No.10379, and preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica.
Chinese herbal medicine in described step (2) is the mixture of more than a kind of or two of which in the Radix Astragali, Flos Lonicerae, Pericarpium Granati, Radix Sanguisorbae, Rhizoma Coptidis, Herba Houttuyniae, Radix Sophorae Flavescentis and Rhizoma Smilacis Glabrae.
The quality of the addition of the Chinese herbal medicine in described step (3) Chinese herbal medicine to add accounts for water gross mass percentages, is 5%～20%.Nutrient in described step (3) is the mixture being made up of glucose, peptone, potassium chloride, magnesium chloride and dipotassium hydrogen phosphate.The quality of the addition of the nutrient in described step (3) nutrient to add accounts for water gross mass percentages, it is respectively as follows: glucose 0.01%～5%, peptone 0.01%～5%, potassium chloride 0.01%～5%, magnesium chloride 0.01%～5%, dipotassium hydrogen phosphate 0.01%～5%.
Bacillus subtilis in described step (5) (Bacillus subtilis) HL-1 seed liquor inoculum concentration with access seed liquor quality account for fermentation medium gross mass percentages, be 0.1%～8%.
Adsorbent used in described step (7) is the one in corn cob, powdered rice hulls, Semen Maydis powder, conch meal and zeolite powder.
The present invention compared with prior art, has the following advantages.
(1) production cost of the green succedaneum of feeding antibiotic is reduced.The present invention produces the method that the bacillus subtilis of antibacterial lipopeptid carries out liquid fermentation, and not only can ferment generation antibacterial lipopeptid, so that medium-height grass the effective elements of the medicine discharges.The fermentation of the fermentation of antibacterial lipopeptid with Chinese herbal medicine is united two into one by the present invention, and the two can share relevant production cost, thus reduces the production cost of the green succedaneum of feeding antibiotic on the whole.
(2) improve the antibacterial activity of antibacterial lipopeptid and Chinese herbal medicine effective ingredients, thus significantly reduce the using dosage of antibacterial lipopeptid and Chinese herbal medicine effective ingredients.The present invention has filtered out its effective ingredient and antibacterial lipopeptid from thousands of medium-height grass Drug Storage and has had the Chinese herbal medicine of collaborative inhibitory effect.The fermentation generation of antibacterial lipopeptid is not only united two into one by the present invention with Chinese herbal medicine fermentation, and due to the interaction of antibacterial lipopeptid Yu Chinese herbal medicine effective ingredients, the antibacterial activity of the two is all obviously enhanced.This is also not available for the most single Chinese herbal medicine fermentation and antibacterial lipopeptid fermenting and producing.The antibacterial activity of prepared antibacterial lipopeptid and Chinese herbal medicine effective ingredients is improve just because of the present invention, so that the using dosage of the feeding antibiotic substitute prepared by the present invention greatly reduces, the actually used dose ratio of antibacterial lipopeptid contained therein and Chinese herbal medicine effective ingredients decreases several times to tens times when being used alone.
Compared with the patent application 201510092596.7 " preparation method of the feed additive of a kind of aquatic product leftovers and Chinese herbal medicine mixed fermentation " that applicant proposes on March 2nd, 2015, in the present patent application, in fermentation raw material, carbon-nitrogen ratio is the normal range of fermenting and producing, this fermenting and producing being more beneficial for lipopeptid and the fermentation of Chinese herbal medicine, thus the feed additive antibacterial activity prepared by technical scheme that the present patent application provides is obviously enhanced.Produce it addition, the present patent application uses liquid fermentation method, it is easier to carry out large-scale production.
The invention provides a kind of rich in lipopeptid with the preparation method of the feeding antibiotic substitute of Chinese herbal medicine effective ingredients.The present invention not only reduces the production cost of feeding antibiotic green succedaneum；But also greatly reduce lipopeptid and the Chinese herbal medicine effective ingredients using dosage at feed industry.The feeding antibiotic substitute rich in lipopeptid and Chinese herbal medicine effective ingredients that the present invention the obtains pig in South China, chicken, the culture experiment of prawn show, the feeding antibiotic substitute prepared by the inventive method can substitute feeding antibiotic completely, substantially reduce aquaculture cost, achieve significant economic benefit and social benefit.
Detailed description of the invention
In order to be more fully understood that the present invention, with regard to the selection of kind, fermentation parameter and fermentation mode of Chinese herbal medicine, the invention will be further described in conjunction with the embodiments below, but protection scope of the present invention is not limited to this.
Embodiment 1: by slant preservation can produce antibacterial lipopeptid bacillus subtilis (Bacillus subtilis) HL-1 is linked into after sterilizing in the LB fluid medium of cooling, in 37 DEG C, 150r/min cultivate 16h, be prepared as bacillus subtilis (Bacillus subtilis) HL-1 seed liquor is standby；60 mesh sieves are crossed standby after being pulverized by the Radix Astragali.10 kilograms of Radixs Astragali pulverized, 500 grams of glucoses, 700 grams of peptones, 600 grams of potassium chloride, 600 grams of magnesium chlorides, 900 grams of dipotassium hydrogen phosphates are mixed together with 100 kg of water, it is thus achieved that the fermentation medium after mixing；Fermentation tank is cultivated based on standby after 121 DEG C of sterilizing 20min；Access in the fermentation tank of sterilizing cooling 3 kilograms of bacillus subtilises (Bacillus subtilis) HL-1 seed liquor；Fermentation temperature is 32 DEG C, and filtrated air intake is 2.0vvm, and the rotating speed of fermentation tank is 400rpm, and fermentation time is 35h；After fermentation ends, fermentation liquor is crossed and is filtered to remove precipitation, collects supernatant；Supernatant is through the 1/3 of evaporation and concentration to original volume；Concentrated solution is that the ratio of 1.5:1 is adsorbed in mass ratio by corn cob；A kind of feeding antibiotic substitute finished product rich in lipopeptid and Chinese herbal medicine effective ingredients can be prepared as after absorption system drying and pulverizing.
Embodiment 2: by slant preservation can produce antibacterial lipopeptid bacillus subtilis (Bacillus subtilis) HL-1 is linked into after sterilizing in the LB fluid medium of cooling, in 37 DEG C, 150r/min cultivate 16h, be prepared as bacillus subtilis (Bacillus subtilis) HL-1 seed liquor is standby；60 mesh sieves are crossed standby after the Radix Astragali and Herba Houttuyniae being pulverized.10 kilograms of composite Chinese herbals (+6 kilograms of Herba Houttuyniae of 4 kilograms of Radixs Astragali), 450 grams of glucoses, 650 grams of peptones, 600 grams of potassium chloride, 600 grams of magnesium chlorides, 900 grams of dipotassium hydrogen phosphates are mixed together with 100 kg of water, it is thus achieved that the fermentation medium after mixing；Fermentation tank is cultivated based on standby after 121 DEG C of sterilizing 20min；Access in the fermentation tank of sterilizing cooling 3 kilograms of bacillus subtilises (Bacillus subtilis) HL-1 seed liquor；Fermentation temperature is 30 DEG C, and filtrated air intake is 2.0vvm, and the rotating speed of fermentation tank is 400rpm, and fermentation time is 42h；After fermentation ends, fermentation liquor is crossed and is filtered to remove precipitation, collects supernatant；Supernatant is through the 1/4 of evaporation and concentration to original volume；Concentrated solution is that the ratio of 1.5:1 is adsorbed in mass ratio by conch meal；A kind of feeding antibiotic substitute finished product rich in lipopeptid and Chinese herbal medicine effective ingredients can be prepared as after absorption system drying and pulverizing.
Claims (8)
1. one kind rich in lipopeptid and the preparation method of the feeding antibiotic substitute of Chinese herbal medicine effective ingredients, it is characterised in that: the method comprises the steps:
(1) by slant preservation can produce antibacterial lipopeptid bacillus subtilis (Bacillus
subtilis) HL-1 is linked into after sterilizing in the LB fluid medium of cooling, in 37 DEG C, 150r/min cultivate 16h, be prepared as bacillus subtilis (Bacillus subtilis) HL-1 seed liquor is standby；
(2) 60 mesh sieves are crossed after being pulverized by Chinese herbal medicine standby；
(3) after step (2) being pulverized, Chinese herbal medicine joins in fermentation tank, and adds water and nutrient in fermentation tank, obtains fermentation medium after mixing；
(4) cultivation in fermentation tank step (3) obtained is based on 121 DEG C of sterilizing 20min；
(5) culture medium in the fermentation tank after sterilizing is cooled to room temperature, then accesses the bacillus subtilis HL-1 seed liquor prepared by step (1)；
(6) control of sweat: fermentation temperature is 30 DEG C～32 DEG C, and filtrated air intake is 0.5vvm～2.5vvm, and the rotating speed of fermentation tank is 150rpm～450rpm, and fermentation time is 35～45h；
(7) product handling system: after fermentation ends, fermentation liquor is crossed and is filtered to remove precipitation, collects supernatant；Supernatant is through being concentrated in vacuo to the 1/3～1/5 of original volume；Then feeding antibiotic substitute finished product can be prepared as after the ratio that concentrated solution and adsorbent are 1～3:1 in mass ratio being carried out mixing and absorption, absorption system drying and pulverizing.
It is the most according to claim 1 a kind of rich in lipopeptid with the preparation method of the feeding antibiotic substitute of Chinese herbal medicine effective ingredients, it is characterised in that: bacillus subtilis used in described step (1) (Bacillus subtilis) HL-1 deposit number at China Committee for Culture Collection of Microorganisms's common micro-organisms center is CGMCC No.10379.
It is the most according to claim 1 a kind of rich in lipopeptid with the preparation method of the feeding antibiotic substitute of Chinese herbal medicine effective ingredients, it is characterised in that: the Chinese herbal medicine in described step (2) is the mixture of more than a kind of or two of which in the Radix Astragali, Flos Lonicerae, Pericarpium Granati, Radix Sanguisorbae, Rhizoma Coptidis, Herba Houttuyniae, Radix Sophorae Flavescentis and Rhizoma Smilacis Glabrae.
The most according to claim 1 a kind of rich in lipopeptid with the preparation method of the feeding antibiotic substitute of Chinese herbal medicine effective ingredients, it is characterized in that: the quality of the addition of the Chinese herbal medicine in described step (3) Chinese herbal medicine to add accounts for water gross mass percentages, is 5%～20%.
It is the most according to claim 1 a kind of rich in lipopeptid with the preparation method of the feeding antibiotic substitute of Chinese herbal medicine effective ingredients, it is characterised in that: the nutrient in described step (3) is the mixture being made up of glucose, peptone, potassium chloride, magnesium chloride and dipotassium hydrogen phosphate.
The most according to claim 1 a kind of rich in lipopeptid with the preparation method of the feeding antibiotic substitute of Chinese herbal medicine effective ingredients, it is characterized in that: the quality of the addition of the nutrient in described step (3) nutrient to add accounts for water gross mass percentages, it is respectively as follows: glucose 0.01%～5%, peptone 0.01%～5%, potassium chloride 0.01%～5%, magnesium chloride 0.01%～5%, dipotassium hydrogen phosphate 0.01%～5%.
It is the most according to claim 1 a kind of rich in lipopeptid with the preparation method of the feeding antibiotic substitute of Chinese herbal medicine effective ingredients, it is characterised in that: bacillus subtilis in described step (5) (Bacillus
subtilis) HL-1 seed liquor inoculum concentration with access seed liquor quality account for fermentation medium gross mass percentages, be 0.1%～8%.
It is the most according to claim 1 a kind of rich in lipopeptid with the preparation method of the feeding antibiotic substitute of Chinese herbal medicine effective ingredients, it is characterised in that: adsorbent used in described step (7) is the one in corn cob, powdered rice hulls, Semen Maydis powder, conch meal and zeolite powder.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510092596.7A CN104719635A (en) | 2015-03-02 | 2015-03-02 | Method for preparing feed additive mixed and fermented by aquatic product leftover and Chinese herbal medicine |
| CN2015100925967 | 2015-03-02 |
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| CN105707453A true CN105707453A (en) | 2016-06-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510092596.7A Pending CN104719635A (en) | 2015-03-02 | 2015-03-02 | Method for preparing feed additive mixed and fermented by aquatic product leftover and Chinese herbal medicine |
| CN201610106541.1A Pending CN105746881A (en) | 2015-03-02 | 2016-02-27 | Method for preparing substituent of antibiotics for feed by fermenting Chinese herbal medicines through lipopeptide-producing bacillus subtilis |
| CN201610112485.2A Pending CN105707453A (en) | 2015-03-02 | 2016-03-01 | Preparing method of feed antibiotic substitute rich in lipopeptid and Chinese herbal medicine effective ingredients |
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| CN201510092596.7A Pending CN104719635A (en) | 2015-03-02 | 2015-03-02 | Method for preparing feed additive mixed and fermented by aquatic product leftover and Chinese herbal medicine |
| CN201610106541.1A Pending CN105746881A (en) | 2015-03-02 | 2016-02-27 | Method for preparing substituent of antibiotics for feed by fermenting Chinese herbal medicines through lipopeptide-producing bacillus subtilis |
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Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104719635A (en) * | 2015-03-02 | 2015-06-24 | 广东海洋大学 | Method for preparing feed additive mixed and fermented by aquatic product leftover and Chinese herbal medicine |
| CN104719636A (en) * | 2015-03-02 | 2015-06-24 | 广东海洋大学 | Method for preparing feed antibiotic substitute by using aquatic product processing wastewater and Chinese herbal medicine |
| CN105211616B (en) * | 2015-10-27 | 2020-01-07 | 广东海洋大学 | Comprehensive utilization method of prawn leftovers |
| CN105707473B (en) * | 2016-03-09 | 2019-08-13 | 广东海洋大学 | A method of the feeding antivirus somatotropic agent of green is prepared using aquatic product protein and Chinese herbal medicine hot fermentation |
| CN105661012B (en) * | 2016-03-09 | 2019-10-29 | 广东海洋大学 | A kind of hot fermentation method of Chinese herbal medicine |
| CN105746886B (en) * | 2016-03-09 | 2019-08-23 | 广东海洋大学 | A method of compound green feed additive for promoting growth is prepared using aquatic product protein and Chinese herbal medicine fermentation |
| CN105724764B (en) * | 2016-03-09 | 2019-10-29 | 广东海洋大学 | A method of antibacterial green feed additive for promoting growth is prepared using aquatic product protein fermentation |
| CN105746889B (en) * | 2016-03-09 | 2019-09-17 | 广东海洋大学 | A method of antibacterial green feed additive for promoting growth is prepared using aquatic product protein two-step fermentation |
| CN105661013B (en) * | 2016-03-09 | 2019-11-19 | 广东海洋大学 | A kind of method that fermentation of Chinese herbal medicine prepares compound feed additive for promoting growth |
| CN109943502B (en) * | 2019-03-04 | 2022-02-01 | 青岛海洋生物医药研究院 | Probiotics, leavening agent and application in Chinese herbal medicine preparation |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102094054A (en) * | 2010-12-07 | 2011-06-15 | 南京农业大学 | Production method of Bacillus subtilis antimicrobial lipopeptide and application of Bacillus subtilis antimicrobial lipopeptide in piglet feeds |
| CN104719635A (en) * | 2015-03-02 | 2015-06-24 | 广东海洋大学 | Method for preparing feed additive mixed and fermented by aquatic product leftover and Chinese herbal medicine |
| CN105211634A (en) * | 2015-10-13 | 2016-01-06 | 延川县富源康茶业专业合作社 | A kind of regulant for animal's growth and preparation method thereof |
| CN105211616A (en) * | 2015-10-27 | 2016-01-06 | 广东海洋大学 | A kind of method of comprehensive utilization to shrimping beam trawl |
-
2015
- 2015-03-02 CN CN201510092596.7A patent/CN104719635A/en active Pending
-
2016
- 2016-02-27 CN CN201610106541.1A patent/CN105746881A/en active Pending
- 2016-03-01 CN CN201610112485.2A patent/CN105707453A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102094054A (en) * | 2010-12-07 | 2011-06-15 | 南京农业大学 | Production method of Bacillus subtilis antimicrobial lipopeptide and application of Bacillus subtilis antimicrobial lipopeptide in piglet feeds |
| CN104719635A (en) * | 2015-03-02 | 2015-06-24 | 广东海洋大学 | Method for preparing feed additive mixed and fermented by aquatic product leftover and Chinese herbal medicine |
| CN105211634A (en) * | 2015-10-13 | 2016-01-06 | 延川县富源康茶业专业合作社 | A kind of regulant for animal's growth and preparation method thereof |
| CN105211616A (en) * | 2015-10-27 | 2016-01-06 | 广东海洋大学 | A kind of method of comprehensive utilization to shrimping beam trawl |
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| CN104719635A (en) | 2015-06-24 |
| CN105746881A (en) | 2016-07-13 |
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