CN105211616A - A kind of method of comprehensive utilization to shrimping beam trawl - Google Patents
A kind of method of comprehensive utilization to shrimping beam trawl Download PDFInfo
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- CN105211616A CN105211616A CN201510705504.8A CN201510705504A CN105211616A CN 105211616 A CN105211616 A CN 105211616A CN 201510705504 A CN201510705504 A CN 201510705504A CN 105211616 A CN105211616 A CN 105211616A
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Abstract
The invention belongs to comprehensive utilization of aquatic products technical field, specifically disclose a kind of method of comprehensive utilization to shrimping beam trawl, further ferment by the shrimping beam trawl inoculation bafillus natto after self-dissolving hydrolysis, shrimp fully can be separated with shrimp shell like this, thus prepare the high shrimp shell meal of purity; Meanwhile, the albumen that feed liquid mid-early stage autolytic process is not hydrolyzed also can fully be hydrolyzed, and obtains the feeding active peptide with remarkable promoting immunity and growth; Because adopted bacillus subtilis subso natto NT-6 bacterial strain is antibacterial lipopeptid high yield strain excellent, a large amount of antibacterial lipopeptids with good characteristic can be produced while fermentation, thus, utilize this technique not only can solve and the difficult problem be separated is difficult to shrimp and shrimp shell in shrimping beam trawl comprehensive utilization, the antibacterial lipopeptid and the feeding active peptide product of nutrition little peptide compound that are rich in high-quality can also be obtained, significantly can reduce production cost, improve the added value to shrimping beam trawl, and realize producing without slag discharge.
Description
Technical field
The present invention relates to comprehensive utilization of aquatic products technical field, more specifically, relate to a kind of method of comprehensive utilization to shrimping beam trawl.
Background technology
Prawn is one of large aquatic products of China, cultured output the first in the world, and its converted products is sold main based on peeled shrimp outlet, therefore can produce in process comprise cannot the low value shrimp of processing and utilization, shrimp shell, shrimp foot and the first-class leftover bits and pieces of shrimp (accounting for whole shrimp 30 ~ 40%), containing crude protein more than 60% (butt) in leftover bits and pieces, also containing abundant astaxanthin, chitin and vitamin B12 etc., be of high nutritive value, therefore prawn shrimp head, shrimp shell are a kind of resources of high-quality.But for a long time, China does not effectively utilize this part resource, except being used on a small quantity produce except fertilizer or feed, major part is thrown away by as rubbish, so not only causes the serious waste of resource, and contaminated environment; How scientifically comprehensive full-scale development utilizes shrimping beam trawl resource, improves its economic worth, becomes problem demanding prompt solution.At present, existing document and patent report, utilize and refine the high-valued products such as astaxanthin, chitin, amino acid to shrimping beam trawl.But because production cost is high, exhaust emission is serious, extensively promotes and is restricted, find be easier to promote, added value is high day by day seems urgent to the comprehensive utilization new method of shrimping beam trawl.
Summary of the invention
Technical problem to be solved by this invention is the above-mentioned defect overcoming prior art existence, provides a kind of method of comprehensive utilization to shrimping beam trawl.
The object of the invention is to be achieved by the following technical programs:
To a method of comprehensive utilization for shrimping beam trawl, comprise the following steps:
S1. by broken for fresh shrimp head powder, after carrying out ultraviolet irradiation, with the speed of 5 DEG C/30min, temperature is risen to 40 ~ 65 DEG C, keep 2 ~ 4h;
S2. going in air blow tank, after adding auxiliary material mixing, sterilizing, is 10 by 1 ~ 5% volume ratio inoculum density
7~ 10
8the bacillus subtilis subso natto NT-6 of CFU/ml starts fermentation;
S3. terminate fermentation, obtain filtrate and filter residue respectively after filtration, filter residue obtains pure shrimp shell meal after washing, drying and crushing; The composite feed active peptide of antibacterial lipopeptid and the little peptide of nutrition is rich in the spray-dried rear acquisition of filtrate; Described bacillus subtilis subso natto NT-6 is stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preserving number is CGMCCNO.8121, and the preservation time is on September 3rd, 2013.
Chitin extraction, astaxanthin are generally used to the utilization of shrimping beam trawl, and when these two kinds of materials of extraction, all need shrimp to be separated with shrimp shell, but when both being separated in factorial praluction, there is certain difficulty, bring obstruction to actual production; Because the institutional framework of prawn is unique, internal organ are positioned at head, wherein containing abundant endogenous enzyme, because the activity of these endogenous enzymes is very high, can produce degradation under certain condition, generate peptide and amino acid etc., and produce self-dissolving protein in leftover bits and pieces; There is this principle of research and utilization to be separated shrimp and shrimp shell by the autolysis of endogenous protease, reclaim the protein component in shrimp head and shrimp shell simultaneously.This processing method plays certain effect aborning, but the leftover bits and pieces after process is still with a large amount of shrimp, and the proteolysis simultaneously reclaimed is also abundant not.
The present invention has done further improvement on the basis of existing technology, by further fermenting to shrimping beam trawl inoculation bacillus subtilis subso natto after self-dissolving hydrolysis, shrimp fully can be separated with shrimp shell like this, thus prepare the high shrimp shell meal of purity; Meanwhile, the albumen that feed liquid mid-early stage autolytic process is not hydrolyzed also can fully be hydrolyzed, and obtains the feeding active peptide with remarkable promoting immunity and growth; Because adopted bacillus subtilis subso natto NT-6 bacterial strain is antibacterial lipopeptid high yield strain excellent, a large amount of antibacterial lipopeptids with good characteristic can be produced while fermentation, thus, utilize this technique not only can solve and the difficult problem be separated is difficult to shrimp and shrimp shell in shrimping beam trawl comprehensive utilization, the antibacterial lipopeptid and the feeding active peptide product of nutrition little peptide compound that are rich in high-quality can also be obtained.
The present invention's fermented bacterium used is bacillus subtilis subso natto (Bacillussubtilissubsp.natto) NT-6 bacterial strain, be stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number is CGMCCNO.8121, preservation address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica; Preservation date is on September 3rd, 2013.
In addition, the heating rate prawn shell in S1 is separated important impact with shrimp, and heating rate is too high, and endogenous enzyme is thorough not to the degradation of shrimp, causes shrimp shell to be separated with shrimp bad; And heating rate is too low, not only play sufficient self-dissolving, shrimp and the adhesion of shrimp shell, also can affect the fermentation of later stage bacillus subtilis subso natto to mash, and we select heating rate to be 5 DEG C/30min here.
Ultraviolet irradiation and the hyperthermic treatment in early stage are in order to self-dissolving, but, consider the fermentation of later stage bacillus subtilis subso natto, the present invention improves in prior art, preferably, in the method for the invention, the time of ultraviolet irradiation described in S1 is 10 ~ 20min.
Preferably, add 1 ~ 3%NaCl after carrying out ultraviolet irradiation in S1, adjust ph is 7 ~ 8.
Preferably, described in S2, auxiliary material described in auxiliary material S2 is selected from molasses or glucose, sodium glutamate, dipotassium hydrogen phosphate, magnesium sulfate; Particularly, described auxiliary material is 0.5 ~ 2.0% molasses, 0.1 ~ 0.5% dipotassium hydrogen phosphate and 0.1% magnesium sulfate.
Later stage fermentation is not only to be separated shrimp and shrimp shell further, also need farthest to obtain the mixture being rich in antibacterial lipopeptid and active small peptide, therefore, here need to control zymotechnique, preferably, the technological parameter fermented described in S2 is: temperature 28 ~ 35 DEG C, speed of agitator 120 ~ 160r/min, throughput 50 ~ 80m
3/ h, tank pressure 0.10 ~ 0.12Mpa, 0.3% defoamer, incubation time 30 ~ 48h.
Compared with prior art, the present invention has following beneficial effect:
The invention provides a kind of method of comprehensive utilization to shrimping beam trawl, further ferment by the mash inoculation bacillus subtilis subso natto after self-dissolving hydrolysis, shrimp fully can be separated with shrimp shell like this, thus prepare the high shrimp shell meal of purity; Meanwhile, the albumen that feed liquid mid-early stage autolytic process is not hydrolyzed also can fully be hydrolyzed, and obtains the feeding active peptide with remarkable promoting immunity and growth; Because adopted bacillus subtilis subso natto NT-6 bacterial strain is antibacterial lipopeptid high yield strain excellent, a large amount of antibacterial lipopeptids with good characteristic can be produced while fermentation, thus, utilize this technique not only can solve and the difficult problem be separated is difficult to shrimp and shrimp shell in shrimping beam trawl comprehensive utilization, the antibacterial lipopeptid and the feeding active peptide product of nutrition little peptide compound that are rich in high-quality can also be obtained, significantly can reduce production cost, improve the added value to shrimping beam trawl, and realize producing without slag discharge.
Detailed description of the invention
Further illustrate content of the present invention below in conjunction with specific embodiment, but should not be construed as limitation of the present invention.Without departing from the spirit and substance of the case in the present invention, the simple modification do the inventive method, step or condition or replacement, all belong to scope of the present invention; If do not specialize, the conventional means that technological means used in embodiment is well known to those skilled in the art.
The cultivation of bacillus subtilis subso natto NT-6:
(1) seed culture medium (g/L): tryptone 10.0g, yeast extract 5.0g, NaCl5.0g, water 1000mL, pH7.2.
(2) expansion of seed is cultivated: after seed culture medium cultivates 18h, (now cell concentration reaches 10 to bacillus subtilis subso natto NT-6 inoculation
7~ 10
8cFU/ml).
embodiment 1
To a method of comprehensive utilization for shrimping beam trawl, specifically comprise the following steps:
S1. by broken for fresh shrimp head powder, carry out ultraviolet irradiation 10min, put into heat insulation tank in 30W uviol lamp, add the water of 1 times of volume, 1%NaCl, adjust ph is 7, with the speed of 5 DEG C/30min, temperature is risen to 40 DEG C, keeps 2h;
S2. go in air blow tank, add the water of 1 times of volume, and add 0.5%(weight ratio) molasses, 0.1%(weight ratio) dipotassium hydrogen phosphate and 0.1%(weight ratio) magnesium sulfate, adjust pH is 7,110 DEG C and keeps 10 ~ 15min sterilizing, by 1% volume ratio inoculation through expanding the bacillus subtilis subso natto NT-6 cultivated, then fermented and cultured is carried out, fermentation parameter is: temperature 30 DEG C, speed of agitator 120r/min, throughput 55m
3/ h, tank pressure 0.10Mpa, 0.3%(weight ratio) defoamer, incubation time 32h.
S3. fermentation is terminated, filtrate and filter residue is obtained respectively with after strainer filtering, filter residue obtains pure shrimp shell meal after washing, drying and crushing, and can be used as the raw material preparing astaxanthin and shitosan, the composite feed active peptide of antibacterial lipopeptid and the little peptide of nutrition is rich in the spray-dried rear acquisition of filtrate; Here spray-dired technique is: temperature 210 DEG C, rotation speed of fan 70 turns/s; Excipient: porous-starch.
The purity of the shrimp shell meal of final acquisition is 96%, and the content of antibacterial lipopeptid is 1200mg/kg, and the content of active small peptide is 210g/kg.
embodiment 2
To a method of comprehensive utilization for shrimping beam trawl, specifically comprise the following steps:
S1. by broken for fresh shrimp head powder, carry out ultraviolet irradiation 15min, put into heat insulation tank in 30W uviol lamp, add the water of 1 times of volume, 2%NaCl, adjust ph is 7.5, with the speed of 5 DEG C/30min, temperature is risen to 50 DEG C, keeps 3h;
S2. go in air blow tank, add the water of 1.5 times of volumes, and add 1%(weight ratio) molasses, 0.3%(weight ratio) dipotassium hydrogen phosphate and 0.1%(weight ratio) magnesium sulfate, adjust pH is 7.3,110 DEG C and keeps 10 ~ 15min sterilizing, by 3% volume ratio inoculation through expanding the bacillus subtilis subso natto NT-6 cultivated, then fermented and cultured is carried out, fermentation parameter is: temperature 32 DEG C, speed of agitator 140r/min, throughput 65m
3/ h, tank pressure 0.11Mpa, 0.3%(weight ratio) defoamer, incubation time 40h.
S3. fermentation is terminated, filtrate and filter residue is obtained respectively with after strainer filtering, filter residue obtains pure shrimp shell meal after washing, drying and crushing, and can be used as the raw material preparing astaxanthin and shitosan, the composite feed active peptide of antibacterial lipopeptid and the little peptide of nutrition is rich in the spray-dried rear acquisition of filtrate; Here spray-dired technique is: temperature 210 DEG C, rotation speed of fan 70 turns/s; Excipient: porous-starch.
The purity of the shrimp shell meal of final acquisition is 99%, and the content of antibacterial little peptide is 1500mg/kg, and the content of active small peptide is 250g/kg.
embodiment 3
To a method of comprehensive utilization for shrimping beam trawl, specifically comprise the following steps:
S1. by broken for fresh shrimp head powder, carry out ultraviolet irradiation 20min, put into heat insulation tank in 30W uviol lamp, add the water of 1 times of volume, 3%NaCl, adjust ph is 8, with the speed of 5 DEG C/30min, temperature is risen to 65 DEG C, keeps 4h;
S2. go in air blow tank, add the water of 2 times of volumes, and add 2%(weight ratio) molasses, 0.5%(weight ratio) dipotassium hydrogen phosphate and 0.1%(weight ratio) magnesium sulfate, adjust pH is 7.5,110 DEG C and keeps 10 ~ 15min sterilizing, by 5% volume ratio inoculation through expanding the bacillus subtilis subso natto NT-6 cultivated, then fermented and cultured is carried out, fermentation parameter is: temperature 35 DEG C, speed of agitator 160r/min, throughput 75m
3/ h, tank pressure 0.10Mpa, 0.3%(weight ratio) defoamer, incubation time 48h.
S3. fermentation is terminated, filtrate and filter residue is obtained respectively with after strainer filtering, filter residue obtains pure shrimp shell meal after washing, drying and crushing, and can be used as the raw material preparing astaxanthin and shitosan, the composite feed active peptide of antibacterial lipopeptid and the little peptide of nutrition is rich in the spray-dried rear acquisition of filtrate; Here spray-dired technique is: temperature 210 DEG C, rotation speed of fan 70 turns/s; Excipient: porous-starch.
The purity of the shrimp shell meal of final acquisition is 97%, and the content of antibacterial little peptide is 1380mg/kg, and the content of active small peptide is 220g/kg.
comparative example 1
Experimental technique with embodiment 2, uniquely unlike, in S1, heating rate is 5 DEG C/3min; Analyze the final product obtained, result shows: the purity of gained shrimp shell meal is 95%, and the content of antibacterial little peptide is 1300mg/kg, and the content of active small peptide is 200g/kg.
comparative example 2
Experimental technique, with embodiment 2, unlike S1 is uniquely: by broken for fresh shrimp head powder, carry out ultraviolet irradiation 30min in 30W uviol lamp, then soaks with the NaCl that concentration is 0.07M, and temperature is the gradient temperature of 50 DEG C (risings per minute 5 DEG C), and the time is 40min.
Analyze the final product that obtains, result shows: the purity of gained shrimp shell meal is 96%, and the content of antibacterial lipopeptid is 1330mg/kg, and the content of active small peptide is 215g/kg.
comparative example 3
Experimental technique is with embodiment 2, and uniquely different unlike fermentation parameter used, fermentation parameter used in this comparative example is: temperature 25 DEG C, speed of agitator 100r/min, throughput 40m
3/ h, tank pressure 0.08Mpa, 0.2% defoamer, incubation time 28h, analyze the final product that obtains, result shows: the purity of gained shrimp shell meal is 92%, and the content of antibacterial lipopeptid is 680mg/kg, and the content of active small peptide is 160g/kg.
Claims (6)
1. to a method of comprehensive utilization for shrimping beam trawl, it is characterized in that, comprise the following steps:
S1. by broken for fresh shrimp head powder, after carrying out ultraviolet irradiation, with the speed of 5 DEG C/30min, temperature is risen to 40 ~ 65 DEG C, keep 2 ~ 4h;
S2. going in air blow tank, after adding auxiliary material mixing, sterilizing, is 10 by 1 ~ 5% volume ratio inoculum density
7~ 10
8the bacillus subtilis subso natto NT-6 of CFU/ml starts fermentation;
S3. terminate fermentation, obtain filtrate and filter residue respectively after filtration, filter residue obtains pure shrimp shell meal after washing, drying and crushing; The composite feed active peptide of antibacterial lipopeptid and the little peptide of nutrition is rich in the spray-dried rear acquisition of filtrate; Described bacillus subtilis subso natto NT-6 is stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preserving number is CGMCCNO.8121, and the preservation time is on September 3rd, 2013.
2. according to claim 1 to the method for comprehensive utilization of shrimping beam trawl, it is characterized in that, the time of ultraviolet irradiation described in S1 is 10 ~ 20min.
3. according to claim 1 to the method for comprehensive utilization of shrimping beam trawl, it is characterized in that, add 1 ~ 3%NaCl after carrying out ultraviolet irradiation in S1, adjust ph is 7 ~ 8.
4. according to claim 1 to the method for comprehensive utilization of shrimping beam trawl, it is characterized in that, auxiliary material described in S2 is selected from molasses or glucose, sodium glutamate, dipotassium hydrogen phosphate, magnesium sulfate.
5. according to claim 4 to the method for comprehensive utilization of shrimping beam trawl, it is characterized in that, described auxiliary material is 0.5 ~ 2.0% molasses, 0.1 ~ 0.5% dipotassium hydrogen phosphate and 0.1% magnesium sulfate.
6. according to claim 1 to the method for comprehensive utilization of shrimping beam trawl, it is characterized in that, the technological parameter fermented described in S2 is: temperature 28 ~ 35 DEG C, speed of agitator 120 ~ 160r/min, throughput 50 ~ 80m
3/ h, tank pressure 0.10 ~ 0.12Mpa, 0.3% defoamer, incubation time 30 ~ 48h.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105685477A (en) * | 2015-03-02 | 2016-06-22 | 广东海洋大学 | Preparation method of antibiotic substitute rich in subtilin and effective Chinese herbal medicine ingredients for feeding |
| CN105707453A (en) * | 2015-03-02 | 2016-06-29 | 广东海洋大学 | Preparing method of feed antibiotic substitute rich in lipopeptid and Chinese herbal medicine effective ingredients |
| CN105724550A (en) * | 2016-03-09 | 2016-07-06 | 广东海洋大学 | Method for performing bone and meat separation on fish wastes by utilizing microbial fermentation |
| CN108991238A (en) * | 2018-07-18 | 2018-12-14 | 岭商（浙江）生物科技有限公司 | A kind of hydrolysis shrimp cream and its production method and application |
| CN109198176A (en) * | 2018-08-13 | 2019-01-15 | 浙江大学 | A kind of method that shrimp shell solid state fermentation prepares culture of microorganism |
| CN114145389A (en) * | 2021-12-24 | 2022-03-08 | 中国海洋大学 | Feed additive and preparation method thereof |
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| CN109198176A (en) * | 2018-08-13 | 2019-01-15 | 浙江大学 | A kind of method that shrimp shell solid state fermentation prepares culture of microorganism |
| CN114145389A (en) * | 2021-12-24 | 2022-03-08 | 中国海洋大学 | Feed additive and preparation method thereof |
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