CN110384178B - Lactic acid bacteria culture prepared based on vinasse and application of lactic acid bacteria culture in animal feed - Google Patents

Lactic acid bacteria culture prepared based on vinasse and application of lactic acid bacteria culture in animal feed Download PDF

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CN110384178B
CN110384178B CN201910786365.4A CN201910786365A CN110384178B CN 110384178 B CN110384178 B CN 110384178B CN 201910786365 A CN201910786365 A CN 201910786365A CN 110384178 B CN110384178 B CN 110384178B
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fermentation
lactobacillus
vinasse
lactic acid
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彭楠
刘玲
常章兵
田建平
梁运祥
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Sichuan Runge Biotechnology Co ltd
Huazhong Agricultural University
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Huazhong Agricultural University
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Abstract

The invention discloses a method for preparing a lactobacillus culture based on white spirit vinasse, which comprises the steps of pretreating the white spirit vinasse with water and sodium bicarbonate, drying and screening rice hulls to obtain the rice hulls and pretreated vinasse; adding 6-8% of lactobacillus fermentation strain and 30-60% of auxiliary material into the pre-treated distiller's grains, and performing anaerobic fermentation at 28-30 deg.C for 72-96h to obtain lactobacillus culture; wherein the auxiliary materials comprise a carbon source and a nitrogen source. The invention also discloses application of the lactobacillus culture in animal feed, and the feed intake and average body weight of animals can be obviously improved by adding the lactobacillus culture prepared by the invention into the animal feed in the form of additives.

Description

Lactic acid bacteria culture prepared based on vinasse and application of lactic acid bacteria culture in animal feed
Technical Field
The invention relates to the technical field of fermentation engineering, in particular to a lactobacillus culture prepared based on vinasse and application thereof in animal feed.
Background
Distiller's grains, namely red distiller's grains, fermented grains, dregs and the like, are residues left after brewing rice, wheat, sorghum and the like. The white spirit vinasse contains a large amount of rice hulls besides residues of grains, and the content of crude protein of the white spirit vinasse is high and can reach more than 20 percent, so that the white spirit vinasse can be added into feed of cattle, sheep or other animals. But the direct feeding of vinasse cannot fully utilize the nutritive value and has poor mouthfeel. Therefore, the distiller's grains are used as a substrate, the lactobacillus is inoculated for lactic acid fermentation, and then the animal is fed with the distiller's grains, so that the nutritional value is utilized to the maximum extent, and the mouthfeel of the animal is better.
The analysis of the fermentation process of the distiller's grains fermented by the microorganisms is developed, the utilization value of the distiller's grains fermented by the microorganisms is improved, the using amount of the distiller's grains in the feed is increased, and the method has important theoretical and practical significance. In the prior art, some patents and other publications disclose that culture is prepared by fermentation of white spirit vinasse and applied to feed, but in the fermentation process, various mixed bacteria are generally adopted: for example, various mixed fermentation cultures of yeast, mould, lactobacillus, bacillus subtilis and the like are complicated and fussy in process, and because the culture conditions of various strains are inconsistent, the final culture can be obtained only by adopting different culture conditions to carry out 2-3 stages of culture; in addition, although a plurality of strains are mixed for fermentation, the content of the final viable bacteria is not high, and analysis shows that the final viable bacteria is not high in quantity because the suitable culture conditions of the plurality of strains are different greatly and part of the strains are dead due to the fact that the strains are not suitable for the environment under different culture conditions, so that the application value of the feed is still to be studied; for the reasons mentioned above, incomplete fermentation of the spent grains may also result, and the resulting increase in protein content of the fermentation culture is not very significant. Therefore, it is necessary to prepare a fermentation culture based on white spirit vinasse for efficient application to animal feed according to the special condition of vinasse acidity.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and to provide at least the advantages described later.
The invention also aims to provide a method for preparing a lactobacillus culture based on white spirit vinasse, which can remove the residual substances such as alcohols, aldehydes and the like by performing awakening pretreatment on the white spirit vinasse, simultaneously adjust the pH value, and supplement a proper amount of carbon source and nitrogen source to create a vinasse culture medium suitable for lactobacillus growth, thereby being beneficial to the growth of lactobacillus and the generation of nutrient substances.
The invention also aims to provide the application of the lactobacillus culture in animal feed, and the lactobacillus culture is added into the animal feed as an additive, so that the utilization rate of the animal to the feed can be obviously improved.
In order to achieve these objects and other advantages of the present invention, there is provided a method for preparing a lactic acid bacteria culture based on white spirit distiller's grains, comprising pretreating white spirit distiller's grains with water and sodium bicarbonate, drying and screening the rice hulls to obtain rice hulls and pretreated distiller's grains;
adding 6-8% of lactobacillus fermentation strain and 30-60% of auxiliary material into the pre-treated distiller's grains, and performing anaerobic fermentation at 28-30 deg.C for 72-96h to obtain lactobacillus culture;
wherein the auxiliary materials comprise a carbon source and a nitrogen source.
Preferably, the pretreatment is performed in a specific manner of: uniformly stirring the white spirit vinasse and 3-5 times of water, adjusting the pH value to 5.5-6.0 by using the sodium bicarbonate, filtering, and collecting solid vinasse;
and drying the solid vinasse at 45-50 ℃ until the water content is 30%, and screening the rice hulls.
Preferably, the auxiliary materials comprise wheat bran, soybean meal and molasses, and the wheat bran is measured by weight: bean pulp: molasses 10-12: 3-5: 3-5;
adding 70-80% of the total amount of the auxiliary materials into the pre-treated distiller's grains, grinding the rest auxiliary materials and the obtained rice hulls into fine powder to be added into a fermentation material together when the fermentation is carried out for 60-72 hours, uniformly stirring, and continuing to ferment until the end.
Preferably, the lactobacillus fermentation strain comprises lactobacillus bulgaricus and lactobacillus acidophilus, the lactobacillus bulgaricus and the lactobacillus acidophilus are subjected to amplification culture by fermentation culture media respectively to obtain a first fermentation liquid and a second fermentation liquid respectively, and the first fermentation liquid and the second fermentation liquid are mixed according to the volume ratio of 1:1 to obtain the lactobacillus fermentation strain;
wherein the fermentation medium comprises the following components in parts by weight: 6-8 parts of vinasse powder, 25-30 parts of corn flour, 15-20 parts of bean pulp, 3-5 parts of dipotassium hydrogen phosphate, 1-3 parts of monopotassium phosphate, 3-5 parts of sodium chloride, 0.5-2 parts of calcium carbonate, 0.05-0.1 part of magnesium sulfate, 0.01-0.05 part of manganese sulfate and 0.01-0.02 part of ferric sulfite; fermentation conditions are as follows: culturing at 28-30 deg.C for 72-96 hr.
Preferably, the order of magnitude of the bacteria content of the lactobacillus fermentation strain is not less than 108 cfu/mL.
Preferably, the distillers' grains powder is prepared by the following method: collecting fresh Chinese liquor distiller's grains, spreading and air drying under ventilation condition to thickness of 10-15cm, standing for 30-40min, directly freeze drying to water content below 12%, and irradiating under ultraviolet condition for 10-15 min.
Preferably, the ultraviolet irradiation conditions are: spreading the lyophilized distiller's grains into 5-10cm thick pieces, spreading after stirring every 2min irradiation under the conditions of wavelength of 254nm and height of 80-100cm, and continuing irradiation for 10 min.
Preferably, the method further comprises the following steps: the obtained lactobacillus culture is sealed and packaged in a sealing bag and stored at a temperature below 25 ℃.
The invention also provides application of the lactobacillus culture in animal feed, wherein the lactobacillus culture prepared by the method is added into the animal feed according to the weight ratio of 6-10 percent, and is used for feeding animals after being uniformly mixed.
The invention at least comprises the following beneficial effects:
the distiller's grains are used as a main raw material, and the fermented distiller's grains also contain residual substances such as alcohols and aldehydes, so that the fermentation is not beneficial to the growth of lactic acid bacteria; then, after drying, screening the rice hulls, which is more beneficial to creating an anaerobic condition, and because the vinasse contains low sugar content and insufficient carbon source, although the vinasse contains higher protein, the carbon source and the nitrogen source are far from insufficient compared with lactobacillus fermentation strains for full fermentation, and the full decomposition of the vinasse and the growth of the lactobacillus are facilitated through the supplement of a proper amount of the carbon source and the nitrogen source; and then a solid state fermentation technology is adopted under a proper temperature condition, a biological fermentation product rich in lactic acid bacteria can be produced, so that the conversion rate of crude protein in the vinasse is effectively improved, the crude fiber content in the vinasse is reduced, and byproducts such as lactic acid and the like are generated to enrich the taste. The product is used as an additive to be added into biological feeds such as cattle and sheep, the palatability of vinasse is improved, the feed intake of animals can be effectively improved, the growth of the animals is promoted, the cost of the complete feed is reduced, toxic substances in the vinasse can be degraded or converted under the action of bacteria, and the safety is higher. Therefore, the lactobacillus culture obtained by the fermentation method with the distiller's grains as the substrate changes the distiller's grains into valuable, reduces the environmental pollution, and more importantly, the additive is applied to the animal feed, so that the utilization rate of the feed can be obviously improved, the growth of animals is promoted, and the method has better economic benefit and social benefit.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The present invention is described in further detail below to enable those skilled in the art to practice the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
The invention provides a method for preparing a lactobacillus culture based on white spirit vinasse, which comprises the steps of pretreating the white spirit vinasse by using water and sodium bicarbonate, drying and screening rice hulls to obtain the rice hulls and pretreated vinasse;
adding 6-8% of lactobacillus fermentation strain and 30-60% of auxiliary material into the pre-treated distiller's grains, and performing anaerobic fermentation at 28-30 deg.C for 72-96h to obtain lactobacillus culture;
wherein the auxiliary materials comprise a carbon source and a nitrogen source.
In a preferred embodiment, the pretreatment is performed in a specific manner: uniformly stirring the white spirit vinasse and 3-5 times of water, adjusting the pH value to 5.5-6.0 by using the sodium bicarbonate, filtering, and collecting solid vinasse;
and drying the solid vinasse at 45-50 ℃ until the water content is 30%, and screening the rice hulls.
In the scheme, the pH value of the solid vinasse is controlled to be 5.5-6.0 after pretreatment, which is the pH range most suitable for the growth of lactic acid bacteria.
And drying at 45-50 ℃, so that the water content of the solid vinasse is reduced to be easy to screen out most of rice husks, part of heat-labile mixed bacteria can be killed, part of mixed bacteria suitable for growth can be prevented from rapidly growing on the machine so as to absorb and utilize part of nutritional ingredients in the mixed bacteria, and the common vinasse contains a small amount of yeast and mould which grow under the condition but are not the optimum growth condition, so that a large amount of yeast and mould can not be generated, and the growth of lactic acid bacteria at the later stage is not influenced.
In a preferred scheme, the auxiliary materials comprise wheat bran, soybean meal and molasses, and the wheat bran is as follows by weight: bean pulp: molasses 10-12: 3-5: 3-5;
adding 70-80% of the total amount of the auxiliary materials into the pre-treated distiller's grains, grinding the rest auxiliary materials and the obtained rice hulls into fine powder to be added into a fermentation material together when the fermentation is carried out for 60-72 hours, uniformly stirring, and continuing to ferment until the end.
In the scheme, most of carbon sources in the vinasse are utilized, so carbon sources suitable for growth of lactic acid bacteria need to be supplemented, nitrogen sources are supplemented in a proper amount according to needs, auxiliary materials are added step by step, the early-stage growth of the lactic acid bacteria mainly depends on sugar decomposition to produce acid so as to reduce the pH value, when the sugar is insufficient, the protein is decomposed, the utilization of the protein in the vinasse is facilitated, the simultaneous supplementation of a large amount of carbon sources is avoided, the carbon sources are only excessive to influence the utilization of the protein in the vinasse, when the fermentation is carried out for 60-72h, the lactic acid bacteria strain reaches a vigorous period, in order to keep a large amount of strains, the residual carbon sources and the nitrogen sources are supplemented, the stirring is uniform, the complete screening cannot be carried out according to the rice hulls, the fermentation products can also be stirred in addition to the secondary supplementation of the carbon sources, the rice hulls are crushed into 800-mesh 1000-mesh powder and then added into the powder, more gaps can be filled, the continuous fermentation of lactic acid bacteria is facilitated, and the rice hulls screened out by scoring can be fully utilized.
In a preferred scheme, the lactobacillus fermentation strain comprises lactobacillus bulgaricus and lactobacillus acidophilus, the lactobacillus bulgaricus and the lactobacillus acidophilus are subjected to amplification culture by fermentation culture media respectively to obtain a first fermentation liquid and a second fermentation liquid respectively, and the first fermentation liquid and the second fermentation liquid are mixed according to a volume ratio of 1:1 to obtain the lactobacillus fermentation strain;
wherein the fermentation medium comprises the following components in parts by weight: 6-8 parts of vinasse powder, 25-30 parts of corn flour, 15-20 parts of bean pulp, 3-5 parts of dipotassium hydrogen phosphate, 1-3 parts of monopotassium phosphate, 3-5 parts of sodium chloride, 0.5-2 parts of calcium carbonate, 0.05-0.1 part of magnesium sulfate, 0.01-0.05 part of manganese sulfate and 0.01-0.02 part of ferric sulfite; fermentation conditions are as follows: culturing at 28-30 deg.C for 72-96 hr.
In the scheme, the lactobacillus fermentation strain comprises lactobacillus bulgaricus and lactobacillus acidophilus, and the fermentation liquid is mixed according to the ratio of 1:1, because the two kinds of bacteria belong to the lactobacillus, the required nutritional conditions are similar, the same culture medium can be adopted for fermentation, the two kinds of bacteria are acid-resistant, and along with the generation of lactic acid in the fermentation process, the existence of the lactobacillus acidophilus is beneficial to the preservation of the viable count.
In a preferable scheme, the order of magnitude of the bacteria content of the lactobacillus fermentation strain is not less than 108cfu/mL。
In a preferred scheme, the vinasse powder is prepared by the following method: collecting fresh Chinese liquor distiller's grains, spreading and air drying under ventilation condition to thickness of 10-15cm, standing for 30-40min, directly freeze drying to water content below 12%, and irradiating under ultraviolet condition for 10-15 min.
In the scheme, the residual alcohol can be volatilized by spreading and drying, the mixed bacteria which are not low-temperature resistant can be destroyed by freeze drying, the cell structure is destroyed, and the ultraviolet irradiation is carried out, so that the mixed bacteria can be thoroughly removed.
In a preferred embodiment, the ultraviolet irradiation conditions are as follows: spreading the lyophilized distiller's grains into 5-10cm thick pieces, spreading after stirring every 2min irradiation under the conditions of wavelength of 254nm and height of 80-100cm, and continuing irradiation for 10 min.
In the scheme, the vinasse is turned, stirred and spread and dried in the air, and the intermittent ultraviolet irradiation is favorable for thoroughly removing the mixed bacteria.
In a preferred embodiment, the method further comprises: the obtained lactobacillus culture is sealed and packaged in a sealing bag and stored at a temperature below 25 ℃.
In the above scheme, the activity and acidity of the lactic acid bacteria in the culture can be maintained under a cool or shady condition at a temperature lower than room temperature.
The invention also provides application of the lactobacillus culture in animal feed, which is characterized in that the lactobacillus culture prepared by the method is added into the animal feed according to the weight ratio of 6-10 percent, and the mixture is uniformly mixed and used for feeding animals.
Lactobacillus bulgaricus and Lactobacillus acidophilus were purchased from Shandong Chuangsheng Probiotics technology Limited.
Example 1
A method for preparing a lactic acid bacteria culture based on white spirit vinasse comprises the following steps:
(1) uniformly stirring the white spirit vinasse by using 3 times of water, adjusting the pH value to 5.5 by using the sodium bicarbonate, filtering, and collecting solid vinasse;
(2) drying the solid vinasse at 45 ℃ until the water content is 30%, and screening rice hulls to obtain rice hulls and pre-treated vinasse;
(3) adding 6% of lactobacillus fermentation strain and 30% of auxiliary material (70% of the total amount) into the pre-treated distiller's grains, maintaining the water content at 55%, performing anaerobic fermentation at 28 deg.C for 60h, adding the rest auxiliary material and 800 mesh rice hull powder, and continuing fermentation until 72h is finished to obtain lactobacillus culture;
wherein, the auxiliary materials are wheat bran: bean pulp: the weight ratio of the molasses is 10: 3: 3, preparing a composition;
the lactobacillus strain comprises lactobacillus bulgaricus and lactobacillus acidophilus, the lactobacillus bulgaricus and the lactobacillus acidophilus are respectively cultured for 72 hours at 28 ℃ in a fermentation culture medium to respectively obtain a first fermentation liquid and a second fermentation liquid, and the first fermentation liquid and the second fermentation liquid are mixed according to the volume ratio of 1:1 to obtain the lactobacillus fermentation strain;
the fermentation medium comprises the following components in parts by weight: 6 parts of vinasse powder, 25 parts of corn flour, 15 parts of soybean meal, 3 parts of dipotassium hydrogen phosphate, 1 part of monopotassium phosphate, 3 parts of sodium chloride, 0.5 part of calcium carbonate, 0.05 part of magnesium sulfate, 0.01 part of manganese sulfate and 0.01 part of ferric sulfite;
the distiller's grains powder is prepared by directly drying fresh distiller's grains to water content of 12%, and grinding into 800 mesh fine powder.
In the above examples, the total viable count of lactic acid bacteria was measured to be 6.4X 10 by the PCA plate assay9cfu/mL; the crude protein content is 25.3%; the content of L-lactic acid is 50.5 g/L.
Example 2
A method for preparing a lactic acid bacteria culture based on white spirit vinasse comprises the following steps:
(1) uniformly stirring the white spirit vinasse with water of which the volume is 4 times that of the white spirit vinasse, adjusting the pH value to 5.8 with the sodium bicarbonate, filtering, and collecting solid vinasse;
(2) drying the solid vinasse at 45 ℃ until the water content is 30%, and screening rice hulls to obtain rice hulls and pre-treated vinasse;
(3) adding 6.8% of lactobacillus fermentation strain and 30% -60% of auxiliary material (75% of the total amount) into the pre-treated distiller's grains, keeping the water content at 58%, performing anaerobic fermentation at 30 ℃ for 72h, adding the rest auxiliary material and 1000 meshes of rice hull powder, and continuing fermentation until 96h to obtain a lactobacillus culture;
wherein, the auxiliary materials are wheat bran: bean pulp: the weight ratio of the molasses is 11: 4: 5, preparing a composition;
the lactobacillus strain comprises lactobacillus bulgaricus and lactobacillus acidophilus, the lactobacillus bulgaricus and the lactobacillus acidophilus are respectively cultured for 96 hours at 30 ℃ in a fermentation medium to respectively obtain a first fermentation liquid and a second fermentation liquid, and the first fermentation liquid and the second fermentation liquid are mixed according to the volume ratio of 1:1 to obtain the lactobacillus fermentation strain;
the fermentation medium comprises the following components in parts by weight: 7 parts of vinasse powder, 30 parts of corn flour, 15 parts of soybean meal, 4 parts of dipotassium hydrogen phosphate, 2 parts of monopotassium phosphate, 4 parts of sodium chloride, 1.5 parts of calcium carbonate, 0.05 part of magnesium sulfate, 0.03 part of manganese sulfate and 0.01 part of ferric sulfite;
the distiller's grains powder is prepared by directly drying fresh distiller's grains to water content of 12%, and grinding into 800 mesh fine powder.
In the above examples, the total viable count of lactic acid bacteria was determined to be 7.5X 10 by the PCA plate assay9cfu/mL; the crude protein content was 28%; the content of L-lactic acid is 58.3 g/L.
Example 3
A method for preparing a lactic acid bacteria culture based on white spirit vinasse comprises the following steps:
(1) uniformly stirring the white spirit vinasse with water of which the volume is 5 times that of the white spirit vinasse, adjusting the pH value to 6.0 with the sodium bicarbonate, filtering, and collecting solid vinasse;
(2) drying the solid vinasse at 50 ℃ until the water content is 30%, and screening rice hulls to obtain rice hulls and pre-treated vinasse;
(3) adding 8% of lactobacillus fermentation strain and 60% of auxiliary materials (80% of the total amount) into the pre-treated distiller grains, keeping the water content at 60%, performing anaerobic fermentation at 30 ℃ for 72h, adding the rest auxiliary materials and 1000 meshes of rice hull powder, and continuing fermentation until 96h to obtain a lactobacillus culture;
wherein, the auxiliary materials are wheat bran: bean pulp: the weight ratio of the molasses is 12: 5: 5, preparing a composition;
the lactobacillus strain comprises lactobacillus bulgaricus and lactobacillus acidophilus, the lactobacillus bulgaricus and the lactobacillus acidophilus are respectively cultured for 96 hours at 30 ℃ in a fermentation medium to respectively obtain a first fermentation liquid and a second fermentation liquid, and the first fermentation liquid and the second fermentation liquid are mixed according to the volume ratio of 1:1 to obtain the lactobacillus fermentation strain;
the fermentation medium comprises the following components in parts by weight: 8 parts of vinasse powder, 30 parts of corn flour, 20 parts of soybean meal, 5 parts of dipotassium hydrogen phosphate, 3 parts of monopotassium phosphate, 5 parts of sodium chloride, 2 parts of calcium carbonate, 0.1 part of magnesium sulfate, 0.05 part of manganese sulfate and 0.02 part of ferric sulfite;
the distiller's grains powder is prepared by directly drying fresh distiller's grains to water content of 12%, and grinding into 800 mesh fine powder.
In the above examples, the total viable count of lactic acid bacteria was measured to be 6.76X 10 by the PCA plate assay method9cfu/mL; the crude protein content was 26.4%; the content of L-lactic acid was 56 g/L.
Example 4
The difference from the example 2 is that the preparation method of the vinasse powder comprises the following steps: collecting fresh Chinese liquor distiller's grains, spreading and air drying under ventilation condition to 10cm thickness, standing for 30min, directly freeze drying to water content below 12%, and irradiating under ultraviolet condition for 10 min.
Wherein, the ultraviolet irradiation conditions are as follows: spreading the lyophilized distiller's grains into 5cm thick, spreading after 2min irradiation once under the conditions of wavelength of 254nm and distance height of 100cm, and continuing irradiation for 10 min.
In the above examples, the total viable count of lactic acid bacteria was measured to be 8.1X 10 by the PCA plate assay9cfu/mL; the crude protein content was 31%; the content of L-lactic acid was 65.8 g/L.
Example 5
A certain farmer in Shaanxi province, Yanyang city, selects 30 white goats with the same weight to perform experiments, randomly divides the white goats into three equal groups, namely, group A, group B and group C, selects common feed purchased from the market, wherein the daily ration adopts a mixture of corn and straw, the common feed is added to the group A on the basis of the daily ration, the common feed mixed with 6% of the lactic acid culture prepared in the embodiment 4 is added to the group B on the basis of the daily ration, the common feed mixed with 10% of the lactic acid culture prepared in the embodiment 4 is added to the group C on the basis of the daily ration, and after the group C is fed for 2 months, compared with the group A, the daily feed intake of the group B and the group C is respectively increased by 25% and 18%; meanwhile, compared with the group A, the average monthly weight gain of the white goats in the group B and the group C is respectively improved by 30-35% and 20-25%.
While embodiments of the invention have been disclosed above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable in various fields of endeavor to which the invention pertains, and further modifications may readily be made by those skilled in the art, it being understood that the invention is not limited to the details shown and described herein without departing from the generic concept as defined by the claims and their equivalents.

Claims (5)

1. A method for preparing a lactobacillus culture based on white spirit vinasse is characterized in that the white spirit vinasse is pretreated by water and sodium bicarbonate, and then rice hulls are dried and screened to obtain the rice hulls and pretreated vinasse; the specific mode of the pretreatment is as follows: uniformly stirring the white spirit vinasse and 3-5 times of water, adjusting the pH value to 5.5-6.0 by using the sodium bicarbonate, filtering, and collecting solid vinasse; drying the solid vinasse at 45-50 ℃ until the water content is 30%, and screening the rice hulls;
adding 6-8% of lactobacillus fermentation strain and 30-60% of auxiliary material into the pre-treated distiller's grains, and performing anaerobic fermentation at 28-30 deg.C for 72-96h to obtain lactobacillus culture; wherein the auxiliary materials comprise a carbon source and a nitrogen source; adding 70-80% of the total amount of the auxiliary materials into the pre-treated distiller's grains, grinding the rest auxiliary materials and the obtained rice hulls into fine powder to be added into a fermentation material together when the fermentation is carried out for 60-72 hours, uniformly stirring, and continuing to ferment until the end;
the lactobacillus fermentation strain comprises lactobacillus bulgaricus and lactobacillus acidophilus, the lactobacillus bulgaricus and the lactobacillus acidophilus are subjected to amplification culture by fermentation culture media respectively to obtain a first fermentation liquid and a second fermentation liquid respectively, and the first fermentation liquid and the second fermentation liquid are mixed according to the volume ratio of 1:1 to obtain the lactobacillus fermentation strain;
the fermentation medium comprises the following components in parts by weight: 6-8 parts of vinasse powder, 25-30 parts of corn flour, 15-20 parts of bean pulp, 3-5 parts of dipotassium hydrogen phosphate, 1-3 parts of monopotassium phosphate, 3-5 parts of sodium chloride, 0.5-2 parts of calcium carbonate, 0.05-0.1 part of magnesium sulfate, 0.01-0.05 part of manganese sulfate and 0.01-0.02 part of ferric sulfite, wherein the fermentation conditions are as follows: culturing at 28-30 deg.C for 72-96 hr;
the order of magnitude of the bacteria content of the lactobacillus fermentation strain is not less than 108 cfu/mL；
The auxiliary materials comprise wheat bran, soybean meal and molasses, and the wheat bran is as follows by weight: bean pulp: molasses 10-12: 3-5: 3-5.
2. The method for preparing a lactic acid bacteria culture based on white spirit stillage according to claim 1, wherein the distillers meal is prepared by the following method: collecting fresh Chinese liquor distiller's grains, spreading and air drying under ventilation condition to thickness of 10-15cm, standing for 30-40min, directly freeze drying to water content below 12%, and irradiating under ultraviolet condition for 10-15 min.
3. The method for preparing a lactic acid bacteria culture based on white spirit distiller's grains according to claim 2, wherein the ultraviolet irradiation conditions are: spreading the lyophilized distiller's grains into 5-10cm thick pieces, spreading after stirring every 2min irradiation under the conditions of wavelength of 254nm and height of 80-100cm, and continuing irradiation for 10 min.
4. The method of preparing a lactic acid bacteria culture based on white spirit stillage of claim 1, further comprising: the obtained lactobacillus culture is sealed and packaged in a sealing bag and stored at a temperature below 25 ℃.
5. The use of a lactic acid bacteria culture in animal feed, characterized in that the lactic acid bacteria culture prepared according to the method of any one of claims 1 to 4 is added to the animal feed in a weight ratio of 6% to 10%, and the mixture is used for feeding animals after being uniformly mixed.
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