CN1239709C - Comprehensive wine residue utilizing process of producing lactic acid and silage - Google Patents
Comprehensive wine residue utilizing process of producing lactic acid and silage Download PDFInfo
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- CN1239709C CN1239709C CNB021381364A CN02138136A CN1239709C CN 1239709 C CN1239709 C CN 1239709C CN B021381364 A CNB021381364 A CN B021381364A CN 02138136 A CN02138136 A CN 02138136A CN 1239709 C CN1239709 C CN 1239709C
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- vinasse
- lactic acid
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention relates to the alcohol field, the environmental protection field, the chemical industry field, the feed field, etc., and more specifically, the present invention comprises: grain stillage generated after alcohol fermentation is carried out is used for producing lactic acid and ensilage through lactic acid bacteria fermentation, so that the goal of comprehensively utilizing grain stillage, reducing alcohol production cost and carrying out clean production and environmental protection. The present invention has the main technical scheme that the grain stillage is used as a culture medium, and lactic acid bacteria strains are inoculated to carry out anaerobic fermentation at the temperature of 28 DEG C to 48 DEG C, the starting pH is from 3.0 to 7.0, and the fermentation time is from 14 hours to 100 hours. The present invention has the advantages that the utilization value of the grain stillage is greatly increased, the production cost of fermentation alcohol is reduced, the COD value of thick mash grain stillage can be effectively reduced by above 50%, and the environment is protected; simultaneously, cheap grain stillage is used as raw materials, the cost of lactic acid is correspondingly reduced, and the development of the chemical industry using the lactic acid as raw materials is promoted.
Description
Technical field
The present invention relates to fields such as alcohol, environmental protection, chemical industry, feed, specifically utilize the vinasse that produce after the zymamsis to produce the method for lactic acid and silage by lactobacillus-fermented.
Technical background
Alcohol is a kind of large leavened prod, occupies an important position in national economy.In recent years, because people are to energy shortage and the earth " Greenhouse effect " aggravation, temperature raises, the understanding of the catastrophic effect that causes is deepened with day aligned, add the use that is under an embargo of reason that some gasoline comburants that many gasoline production and supplier advertise in recent years such as methyl tertiary butyl ether (MTBE) etc. are harmful to health because of polluted underground water etc., utilize alcohol partly to substitute gasoline and also become more surging as the cry of liquid fuel and gasoline comburant as bioenergy.The development fuel alcohol not only can consume starch-containing raw materials such as a large amount of corns, promotes the sustainable development of agricultural planting industry; And can be used as the pollution that clean fuel substitutes gasoline or alleviates vehicle exhaust as gasoline dope, protect environment.Simultaneously, also can alleviate the intake pressure of strategic materials crude oil.China formally comes into effect the fuel alcohol plan.Exploitation fuel alcohol subject matter is that the fuel alcohol price is higher than gas price, is open question still in the world.At present, the price of fuel alcohol is higher than about 1200 yuan/ton of gas prices, and how reducing the zymamsis production cost is the focus of research.Simultaneously, fermentation alcohol can produce big quantity of fluid vinasse, and as not handling and utilizing, these vinasse will cause serious pollution to environment.At present, the vinasse in the unprocessed discharging of China grain distillery have become the second largest source of pollution of light industry.But more existing vinasse treatment processs exist investment big respectively, and weak points such as energy consumption height or poor processing effect have had a strong impact on applying of these technology.
Lactic acid (Lactic acid) is one of a kind of edible, the most general organic acid that mouthfeel is moderate.The lactic acid chemical name is a 2 hydroxy propanoic acid, is colourless or faint yellow maltose shape thick liquid, relative density 1.2485 (15 ℃), and fusing point is 16.8 ℃, 122 ℃ (1.995 * 10 of boiling point
3Handkerchief) the lactic acid water-absorbent is strong, in soluble in water, ethanol, ether and the glycerine, is insoluble to benzene and chloroform.As one of three big organic acids, multiple fields such as lactic acid is widely used in and brewages, medicine, leather, cigarette, chemical industry, food, printing and dyeing, can be used as sanitas, acidic flavoring agent, flavour agent, disinfectant etc., along with improving constantly of people's living standard, the new purposes of lactic acid finds that constantly its demand also increases day by day.Lactic acid molecules contains a unsymmetrical carbon, therefore has opticity, and optical isomer is arranged, and is divided into the D-type, L-type, DL-type.Because human body and animal only contain the L-serum lactic dehydrogenase, thereby decompose the L-lactic acid that produces from body or take in.L-lactic acid removes directly as sterilizing agent aspect medical, also can be made into L-Sodium.alpha.-hydroxypropionate, L-lactic acid and make transfusion with glucose, amino acid etc. are composite, but therapic acid is poisoned and high false mass formed by blood stasis.The Application Areas that L-lactic acid is opened up energetically is to produce poly (l-lactic acid).Poly (l-lactic acid) belongs to nontoxic macromolecular compound, has biocompatibility.And poly (l-lactic acid) is biodegradable, makes plastics, causes " white pollution " unlike PVC, PP plastics.Therefore L-lactic acid also is the focus of present scientific research.
Ensiling (silage) feed is meant a kind of nutritious, succulence, anti-storage that fresh green fodder forms through lactobacillus-fermented, can is a kind of mode that milk-acid bacteria is widely used in feed for annual feed of feeding.Because the growth and breeding of milk-acid bacteria and the generation of multiple meta-bolites, can preserve and improve the nutrient in the green fodder, also can suppress the growth of spoilage microorganisms simultaneously,, silage fresh in long time maintenance is the effective ways of feeds such as modulation storage green fodder and stalk, is the practical and effective measure that develops stock raising and produce.
Technology contents
The objective of the invention is to seek the method that lactic acid and silage are produced in a kind of vinasse comprehensive utilization, to solve the deficiency of present vinasse treatment process, passive processing is become initiatively effectively comprehensive utilization, thereby reduce the Alcohol Production cost, the protection environment.Produce high value added product lactic acid and feed---silage efficiently simultaneously, promote livestock industry and be the chemical industry of raw material with lactic acid.
According to design provided by the invention, with vinasse is substratum, in vinasse, add glucose or starch material earlier, wherein the add-on of glucose is 0.2~4.0% of vinasse, the add-on of starch is 1.0~4.0% of vinasse, adds nitrogenous source again in vinasse, and its add-on is 0.5~1.0% of vinasse, and then in vinasse, adding corn steep liquor, add-on is 0.15~0.75% of vinasse; In the time of 28~48 ℃, insert lactobacillus inoculation again, carry out anaerobically fermenting, initial pH3.0~7.0, fermentation time is 14~100 hours, fermentation ends obtains fermented liquid; Utilize fermented liquid to the feed ensiling again, produce silage; Or utilize broth extraction lactic acid; Unit is a mass percent.
Before inserting lactobacillus inoculation, can in vinasse, add glucose or starch material, its add-on is 0~4.0% of vinasse, unit is a mass percent.
Before inserting lactobacillus inoculation, also can in vinasse, add nitrogenous source again, its add-on is 0~2.0% of vinasse.
Described nitrogenous source is an ammonium sulfate, and add-on is 0~1.0% of vinasse; Or/and the ammonium chloride add-on is 0~1.0% of vinasse; Or/and volatile salt, add-on is 0~1.0% of vinasse; Or/and urea, add-on is 0~0.5% of vinasse; Or/and fish meal, add-on be vinasse 0~0.75% or/and soybean cake powder, add-on is 0~2.0% of vinasse, unit is a mass percent.
Before inserting lactobacillus inoculation, in vinasse, add corn steep liquor again, add-on is 0~1.0% of vinasse.
Before inserting lactobacillus inoculation, in vinasse, also add inorganic salt, add-on is 0~0.5% of vinasse.
Used inorganic salt are potassium primary phosphate, and add-on is 0~0.5% of vinasse; Or/and dipotassium hydrogen phosphate, add-on is 0~0.5% of vinasse; Or/and sodium-chlor, add-on is 0~0.5% of vinasse.
Described lactobacillus inoculation can be to be applicable to the lactobacillus inoculation of producing lactic acid.Lactobacillus inoculation adopts liquid fermenting to carry out I and II and spreads cultivation, and seed culture medium is vinasse.The inoculum size of fermentations at different levels is 5~15%, and unit is a percent by volume.
Fermentation time of the present invention can be divided into two sections in 14~100 hours, and promptly 14~32 hours, the gained fermented liquid was used for silage; 45~100 hours, fermentation ends obtains can be for the fermented liquid that extracts lactic acid.
The present invention can add lime carbonate during the fermentation also can not add lime carbonate, if add lime carbonate then added every 10~24 hours during the fermentation, addition decide (adding common method calculating by lime carbonate in the present lactic acid-producing) according to concrete fermentation appearance.When producing lactic acid, generally need to add lime carbonate; When producing silage, in lactic fermentation process, do not need to add lime carbonate.
Vinasse of the present invention both can be distiller's grains, can use common vinasse, also can be the vinasse that raw material fermentation obtains.Described distiller's grain is meant and adopts the described zymamsis technology of patent ZL96117189.8 and adopt the described technology of patent ZL96117189.8 to carry out the vinasse that zymamsis obtained with other cereal and potato starch raw material that the ZL96117189.8 patent does not comprise.Described common vinasse are meant the vinasse that adopt traditional zymamsis (more than or equal to 3: 1, residual total reducing sugar was smaller or equal to 18 grams per liter karusens as water addition ratio) to be produced.Producing the appropriate to the occasion distiller's grain of use of lactic acid, can be with the also available distiller's grain of common vinasse when the production silage.Described raw material fermentation is at present conventional alcohol raw material liquid fermenting basis, reduces water addition ratio to smaller or equal to the zymamsis of carrying out after 2.5.
The present invention adopts present normally used lactic acid extraction technology to broth extraction lactic acid.
The present invention adopts present normally used Silaging method to the Silaging method of feed.
All zymamsiss of the present invention all are meant and utilize yeast to carry out zymamsis; Described all zymamsiss all do not comprise solid-state zymamsis.
Unit of the present invention does not have specified otherwise and is mass percent.
The invention has the advantages that: 1. the present invention has improved the utility value of vinasse greatly, reduces the production cost of fermentation alcohol; 2. the present invention COD more than 50% that can effectively reduce distiller's grain can realize the zero release of vinasse substantially if combine with the total reflux technology of zymamsis, effectively protects environment; 3. milk-acid bacteria mainly relies on the utilized material in the vinasse to keep growth, breed required nutrition among the present invention, other additive consumption seldom, and vinasse are very cheap, therefore reduced the production cost of lactic acid and silage, promoted livestock industry and be the development of the chemical industry of raw material with lactic acid; 4. the scope of initial pH is wide in the production technique of the present invention, and the pH of distiller's grain is generally about 4.5, and therefore not needing generally speaking or only need will be with a small amount of acid-alkali accommodation pH, and the consumption of saving soda acid reduces cost; 5. because zymamsis only utilizes the most of starch in the raw material, solid substance composition in the poor liquid mainly is rich nutrient substances such as unemployed carbohydrate, fat, ash content, amino acid and multivitamin, the yeast used of the zymamsis of rich in proteins is also stayed in the vinasse in addition, therefore to the feed ensiling, the nutrition of gained silage is abundanter behind the lactobacillus-fermented certain hour for vinasse.
Embodiment
Below carry out the description of embodiment with regard to the present invention, wherein, except that vinasse, the add-on of all the other materials all refers to based on the distiller's grain add-on, the mass percent of converting out, the density of vinasse is to be similar to 1 kg/liter.Specify as nothing, vinasse described in the embodiment are common vinasse.Specific embodiment is as follows:
Embodiment 1: based on the vinasse of maize raw material alcohol thick mash fermentation, insert lactobacillus inoculation, and initial pH5.5, at 37 ℃ of anaerobically fermentings, fermentation 24hr.Get fermented liquid to the feed ensiling, ensiling is respond well.
Embodiment 2: based on the vinasse of cassava raw material alcohol thick mash fermentation, add 1.5% starch, and 2.5% glucose, 2.0% soybean cake powder inserts lactobacillus inoculation, and initial pH5.8 exists, 40 ℃ of following anaerobically fermentings, fermentation 63hr.Fermenting process added lime carbonate every 12 hours, and the addition of lime carbonate is 8 ± 2 grams per liter fermented liquids.After the fermentation ends, fermented liquid lactic acid content 74.2 grams per liter fermented liquids, the COD clearance reaches 55.27%.
Embodiment 3: based on the vinasse of maize raw material alcohol thick mash fermentation, adds 4.0% starch, inserts lactobacillus inoculation, and initial pH3.0, at 28 ℃ of anaerobically fermentings, fermentation 100hr.Fermenting process added lime carbonate every 24 hours, and the addition of lime carbonate is 9 ± 2 grams per liter fermented liquids.After the fermentation ends, fermented liquid lactic acid content 66.7 grams per liter fermented liquids, the COD clearance reaches 53.31%.
Embodiment 4: based on the vinasse of maize raw material alcohol raw material fermentation, add 1.0% ammonium sulfate, 0.5% corn steep liquor inserts lactobacillus inoculation, initial pH6.0, and at 37 ℃ of following anaerobically fermentings, fermentation 88hr.Fermenting process added lime carbonate every 17 hours, and the addition of lime carbonate is 8.5 ± 2 grams per liter fermented liquids.After the fermentation ends, fermented liquid lactic acid content 78.3 grams per liter fermented liquids, the COD clearance reaches 61.49%.
Embodiment 5: based on the vinasse of maize raw material alcohol thick mash fermentation, adds 1.0% starch, inserts lactobacillus inoculation, and initial pH3.0, at 37 ℃ of anaerobically fermentings, fermentation 28hr.Get fermented liquid to the feed ensiling, ensiling is respond well.
Embodiment 6: the vinasse based on cassava, the fermentation of melon dry blending raw material alcohol, add 0.5% urea, and 1.0% corn steep liquor inserts lactobacillus inoculation, initial pH5.5, at 48 ℃ of following anaerobically fermentings, fermentation 14hr.Get fermented liquid to the feed ensiling, ensiling is respond well.
Embodiment 7: based on the vinasse of wheat materials zymamsis, add 0.2% glucose, 1.0% volatile salt, 0.25% corn steep liquor, 0.5% sodium-chlor, 0.15% potassium primary phosphate, 0.25% dipotassium hydrogen phosphate inserts lactobacillus inoculation, initial pH7.0, at 32 ℃ of following anaerobically fermentings, fermentation 32hr.Get fermented liquid to the feed ensiling, ensiling is respond well.
Embodiment 8: the vinasse of cassava raw material alcohol fermentation are the basis, add 0.35% soybean cake powder, 1.0% ammonium chloride, and 0.5% dipotassium hydrogen phosphate, 0.05% sodium-chlor inserts lactobacillus inoculation, at 42 ℃ of following anaerobically fermentings, initial pH6.5, fermentation 20hr.Get fermented liquid to the feed ensiling, ensiling is respond well.
Embodiment 9: based on the vinasse of cassava raw material alcohol thick mash fermentation, add 4.0% glucose, and 0.5% soybean cake powder, 0.15% corn steep liquor, 0.5% potassium primary phosphate inserts lactobacillus inoculation, and initial pH5.8 at 45 ℃ of following anaerobically fermentings, fermented 45 hours.Fermenting process added lime carbonate every 10 hours, and the addition of lime carbonate is 8.5 ± 2 grams per liter fermented liquids.After the fermentation ends, fermented liquid lactic acid content 68.9 grams per liter fermented liquids, the COD clearance reaches 53.46%.
Embodiment 10: based on the vinasse of wheat materials alcohol thick mash fermentation, add 1.2% starch, and 0.8% glucose, 0.75% fish meal, 0.25% ammonium chloride, 0.75% corn steep liquor, initial pH4.5 inserts lactobacillus inoculation, at 32 ℃ of following anaerobically fermentings, fermentation 78hr.Fermenting process added lime carbonate every 15 hours, and the addition of lime carbonate is 7 ± 2 grams per liter fermented liquids.After the fermentation ends, fermented liquid lactic acid content 65.1 grams per liter fermented liquids, the COD clearance reaches 60.11%.
Claims (5)
1, the method for a kind of vinasse comprehensive utilization production lactic acid and silage, it is characterized in that, with vinasse is substratum, add glucose or starch material earlier in vinasse, wherein the add-on of glucose is 0.2~4.0% of vinasse, and the add-on of starch is 1.0~4.0% of vinasse, in vinasse, add nitrogenous source again, its add-on is 0.5~1.0% of vinasse, and then adds corn steep liquor in vinasse, and add-on is 0.15~0.75% of vinasse; In the time of 28~48 ℃, insert lactobacillus inoculation again, carry out anaerobically fermenting, initial pH3.0~7.0, fermentation time is 14~100 hours, fermentation ends obtains fermented liquid; Utilize fermented liquid to the feed ensiling again, produce silage; Or utilize broth extraction lactic acid; Unit is a mass percent.
2, the method that lactic acid and silage are produced in described vinasse comprehensive utilization according to claim 1, it is characterized in that: before inserting lactobacillus inoculation, add inorganic salt again in vinasse, add-on is 0~0.5% of vinasse, and unit is a mass percent.
3, the method that lactic acid and silage are produced in described vinasse comprehensive utilization according to claim 2, it is characterized in that: used inorganic salt are potassium primary phosphate, add-on is 0~0.5% of vinasse; Unit is a mass percent.
4, the method that lactic acid and silage are produced in described vinasse comprehensive utilization according to claim 2, it is characterized in that: used inorganic salt are potassium primary phosphate, dipotassium hydrogen phosphate and sodium-chlor, unit is a mass percent.
5, the method that lactic acid and silage are produced in described vinasse comprehensive utilization according to claim 2, it is characterized in that: used inorganic salt are dipotassium hydrogen phosphate and sodium-chlor, unit is a mass percent.
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CNB021381364A CN1239709C (en) | 2002-08-16 | 2002-08-16 | Comprehensive wine residue utilizing process of producing lactic acid and silage |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101818175A (en) * | 2010-04-28 | 2010-09-01 | 江苏绿丰生物药业有限公司 | Method for preparing L-lactic acid through mixed fermentation of distiller grain and jinggangmycin fermentation residues |
Families Citing this family (10)
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DK2361507T3 (en) * | 2006-07-14 | 2016-08-01 | Legarth Lone | A process for the preparation of homo fermented feed products |
CN101919491A (en) * | 2009-06-16 | 2010-12-22 | 管新飞 | Method for processing highland barley lees powder |
CN102640852B (en) * | 2012-05-28 | 2013-07-31 | 安徽农业大学 | Vegetable soybean hull silage and preparation method thereof |
CN103330050A (en) * | 2013-05-10 | 2013-10-02 | 张永根 | Corn steep liquor and rice straw mixed fermentation feed production technology |
CN103420489A (en) * | 2013-07-09 | 2013-12-04 | 湖南工业大学 | Novel method for producing organic acid by using industrial wastewater produced in food fermentation process |
CN103829042B (en) * | 2014-03-26 | 2016-05-25 | 广西新天德能源有限公司 | A kind of production method of polyvitmin active protein cassava feed |
CN104232550B (en) * | 2014-09-29 | 2016-10-19 | 安阳工学院 | A kind of lactic acid bacteria enriched medium and preparation method thereof |
CN105994945B (en) * | 2016-06-03 | 2019-11-08 | 山东省农业科学院畜牧兽医研究所 | A kind of lactobacillus-fermented feed and preparation method thereof |
CN111100803B (en) * | 2018-10-26 | 2022-05-17 | 财团法人农业科技研究院 | Method for culturing acid-resistant lactic acid bacteria and application thereof |
CN110384178B (en) * | 2019-08-23 | 2021-04-20 | 华中农业大学 | Lactic acid bacteria culture prepared based on vinasse and application of lactic acid bacteria culture in animal feed |
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2002
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101818175A (en) * | 2010-04-28 | 2010-09-01 | 江苏绿丰生物药业有限公司 | Method for preparing L-lactic acid through mixed fermentation of distiller grain and jinggangmycin fermentation residues |
CN101818175B (en) * | 2010-04-28 | 2012-07-04 | 江苏绿叶农化有限公司 | Method for preparing L-lactic acid through mixed fermentation of distiller grain and jinggangmycin fermentation residues |
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