CN1141884C - Microbial silage additive and its preparation method - Google Patents
Microbial silage additive and its preparation method Download PDFInfo
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- CN1141884C CN1141884C CNB021129096A CN02112909A CN1141884C CN 1141884 C CN1141884 C CN 1141884C CN B021129096 A CNB021129096 A CN B021129096A CN 02112909 A CN02112909 A CN 02112909A CN 1141884 C CN1141884 C CN 1141884C
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- fermentation
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Abstract
The present invention relates to a microorganism ensilage additive and a preparation method thereof, which belongs to the technical field of ensilage feed additives in the feed industry, and more specifically belongs to a fermentation promoter in the technical field of ensilage feed additives by classification. The method of the present invention comprises: lactobacillus is used as initial fermentation strains, B. longum is used as subsequent fermentation strains, and the microorganism ensilage additive is prepared by using the secondary fermentation technology and the freezing keeping alive post processing technology. In the fermentation process of the microorganism ensilage additive, optimized seed culture medium is adopted, fermentation tank culture medium is adopted, fermentation accelerating agents, fermentation activating agents and cryoprotective agents are added, so that the activity and the high efficiency of the product of the present invention are ensured, and the fermentation mode of the ensilage is effectively controlled. The development and the application of the product can develop feed resources, increase the nutritional quality of the ensilage, and improve the palatability, and has important effect on the development of the feed industry and the animal husbandry.
Description
Technical field
The present invention relates to a kind of microbial silage additive and preparation method thereof, belong to the silage additive technical field in feed industry, more specifically classification belongs to fermentation accelerant wherein.
Background technology
Feed industry is to be the comprehensive industry of raw material to utilize grain and accessory substance thereof, and the development feed industry is to strengthen the grain comprehensive utilization, saves food most important and valid approach.Feed accounts for about 70% of aquaculture production cost, and the development of feed industry is the principal element of animal husbandry scientific and technological progress.
China is large agricultural country, and annual about 5.7 hundred million tons of the various straws green and rough feeds such as (as wheat straw, straw, corn stalk) of producing wherein only about 25% is used as the cattle and sheep feed, causes the significant wastage of potential feed resource.Experiment confirm, is reasonably processed, is handled green and rough feeds and can improve its nutritive value greatly science, and the ensiling technology is effective.But it is big that traditional ensiling technology nutriment in ensilage loses, and opens easily corruption of back, cellar for storing things.Utilize the microorganism formulation that is specifically designed to ensiling of modern biotechnology development can overcome the problems referred to above effectively.
Domestic and international research to silage additive, mainly contain two aspects: the one, outer adding preservative agent (formaldehyde, sulfurous acid etc.), organic acid (formic acid) or inorganic acid (sulfuric acid, hydrochloric acid etc.), promptly belong to the interpolation fermentation inhibitor, stopping the activity of microorganism in the greenfeed, thereby reach the purpose of protecting the root of Dahurian angelica.Its advantage is to make the degree that the pH value of ensilage drops to be needed at the very start, thereby gets rid of or reduce the loss of some good gas and anaerobic fermentation in the ensilage.Shortcoming is that mineral acid may influence the mineral metabolism in the animal body.In addition, because the general negligible amounts of additional substance is higher to the specification requirement that ensilage mixes.The 2nd, microorganism ensiling Study on Technology promptly belongs to the fermentation accelerant technical field.Its technical essential is that the quick adjustment pH value reduces the consumption of nutriment in the ensilage as much as possible to optimum range, thereby reaches the purpose that improves the ensilage nutrient composition content indirectly; Emphasize ensiling quality and ensilage digestion and metabolism in animal body.Its difficult point is the seed selection of high yield lactic bacteria strain, compatibility and fermentation condition, processes keep-alive techniques after adding fermentation activator and suitable product.
The domestic patent of still not having at present microorganism ensiling agent aspect, the ensiling agent patent of the U.S. is more: as U.S.Pat No.4842871,4981705,5747020,6054148 etc.
The purpose of this invention is to provide a kind of microbial silage additive and preparation method thereof.Can improve ensiling straw lactic acid content 10% after adopting micro storage additive agent of the present invention, reduce pH value 0.25, improve the quality and the stability of ensilage greatly.
Particular content of the present invention:
A kind of microbial silage additive; it is to adopt Bacillus acidi lactici (Lactobacillus) as initial fermented bacterium;, make through secondary fermentation and through processing after the bacterial classification keep-alive as follow-up fermented bacterium with long Bifidobacterium Bifidum (B.longum), viable count is greater than 10 in the product 1 year
10/ gram can improve ensiling straw lactic acid content 10% after the interpolation, reduce pH value 0.25.
The preparation method of product of the present invention is
(1) bacterial classification
With Bacillus acidi lactici (Lactobacillus) as initial fermented bacterium, with long Bifidobacterium Bifidum (B.longum) as follow-up fermented bacterium.
(2) zymotechnique
Seed culture medium is formed (g/100ml):
Tryptone 1, lactose 0.5, beef extract 0.4, yeast extract 0.3, NaCl 0.4, bromocresol purple 0.3, Tween-80 0.1, L-HCl cysteine 0.1, culture medium PH7.0,115 ℃ of sterilizations 15 minutes.
Fermentation tank culture medium is formed (g/100ml)
Peptone 2.0, glucose 1.0, yeast juice 0.5, beef extract 0.5, L-HCl cysteine 0.1, culture medium PH7.0,115 ℃ of sterilizations 15 minutes.
Condition of culture
Seed culture: be shake flask fermentation, get the bottled liquid 30ml of 250ml triangle, by 10% inoculum concentration, 30 ℃, the 220r/min rotary shaker was cultivated 36 hours.
Fermentation tank culture: the 15L canned liquid 6-7L that ferments automatically, by 5% inoculum concentration, 30 ℃, 550r/min, ventilation 1vvm cultivates 28～32h.
(3) composite preparation microbial silage additive
The zymotic fluid that two kinds of bacterial classifications are made respectively carries out composite, and its proportioning is: the initial zymotic fluid of Bacillus acidi lactici: the follow-up zymotic fluid of long Bifidobacterium Bifidum is 40～60: 60～40 (W/W).
Add calcium salt and metal salt compound and promote activator as fermentation, it consists of (the composite zymotic fluid of g/100ml): Triammonium citrate 2.0, FeSO
47H
2O 0.035, MgSO
47H
2O 0.575, MnCl
24H
2O 0.11, KH
2PO
40.3, CaCl
20.4.
Add polysaccharide compound as cryoprotective agent, its addition is (percetage by weight of composite zymotic fluid): trehalose 0.1, sorbose 0.1.
(4) process after the keep-alive
0～-4 ℃ of freezing keep-alive down, store standby products obtained therefrom.
Advantage of the present invention is that the Application and Development of microbial silage additive can be utilized feedstock production ensilages such as crop stalk, herbage, hydrophyte fodder, the exploitation feed resource, improve efficiency of feed utilization, more herbvore domestic animals of developing grain-saving type are quickly improved meat product output.
Microbial silage additive can be controlled the ensilage fermentation pattern, reduces the loss of ensilage nutriment in ensilage, improves palatability, reduces toxic and side effect, prevents corrupt histamine and the mycotoxin that produces.
The application of microbial silage additive is to solve plant-eating animal roughage shortage of resources, save the feed consumption, improve the effective way of ensilage nutritional quality, product stability and livestock-raising level, can strengthen the competitiveness in the international market of China's feed industry and livestock products, will make contributions for feed industry and Developing of Animal Industry.
The specific embodiment
Embodiment 1.
With the above-mentioned culture medium of this specification, adopt Lactobacillus as initial fermented bacterium, carry out seed culture and fermentation tank culture, make initial zymotic fluid.
Carry out seed culture and fermentation tank culture with B.longum as follow-up fermented bacterium in addition, make follow-up zymotic fluid.
With weight ratio 50: 50, two kinds of zymotic fluids are composite, and add as fermentation that this specification is above-mentioned promotes activator and cryoprotective agent, product is-4 ℃ times freezing keep-alives.
Embodiment 2.
With weight ratio 60: 40 that two kinds of zymotic fluids are composite, its fermentation condition, additive, freezing keep-alive condition are all with embodiment 1.
Claims (2)
1. microbial silage additive; it is characterized in that adopting Bacillus acidi lactici (Lactobacillus) as initial fermented bacterium; with long Bifidobacterium Bifidum (B.longum) as follow-up fermented bacterium; through secondary fermentation and through processing after the bacterial classification keep-alive and make, viable count is greater than 10 in the product 1 year
10/ gram can improve ensiling straw lactic acid content 10% after the interpolation, reduce pH value 0.25.
2. the preparation method of a microbial silage additive is characterized in that:
(1) bacterial classification
With Bacillus acidi lactici (Lactobacillus) as initial fermented bacterium, with long Bifidobacterium Bifidum (B.longum) as follow-up fermented bacterium;
(2) zymotechnique
Seed culture medium is formed (g/100ml):
Tryptone 1, lactose 0.5, beef extract 0.4, yeast extract 0.3,
NaCl 0.4, bromocresol purple 0.3, and Tween-80 0.1, L-HCl cysteine 0.1, culture medium PH7.0,115 ℃ of sterilizations 15 minutes,
Fermentation tank culture medium is formed (g/100ml):
Peptone 2.0, glucose 1.0, yeast juice 0.5, beef extract 0.5, L-HCl cysteine 0.1,115 ℃ of sterilizations of culture medium PH7.0 15 minutes,
Condition of culture
Seed culture: be shake flask fermentation, get the bottled liquid 30ml of 250ml triangle, by 10% inoculum concentration, 30 ℃, the 220r/min rotary shaker was cultivated 36 hours,
Fermentation tank culture: the 15L canned liquid 6～7L that ferments automatically, by 5% inoculum concentration, 30 ℃, 500r/min, ventilation 1vvm cultivates 28～32h,
(3) composite preparation microbial silage additive
The zymotic fluid that two kinds of bacterial classifications are made respectively carries out composite, and its proportioning is
The initial zymotic fluid of Bacillus acidi lactici: the follow-up zymotic fluid of long Bifidobacterium Bifidum is 40～60: 60～40 (W/W)
Add calcium salt and metal salt compound and promote activator as fermentation, it consists of (the composite zymotic fluid of g/100ml): Triammonium citrate 2.0, FeSO
47H
2O 0.035, MgSO
47H
2O 0.575, MnCl
24H
2O 0.11, KH
2PO
40.3, CaCl
20.4,
Add polysaccharide compound as cryoprotective agent, its addition is (percetage by weight of composite zymotic fluid): trehalose 0.1, and sorbose 0.1,
(4) process after the keep-alive: 0～-4 ℃ of freezing keep-alive down, store standby products obtained therefrom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021129096A CN1141884C (en) | 2002-04-18 | 2002-04-18 | Microbial silage additive and its preparation method |
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| CNB021129096A CN1141884C (en) | 2002-04-18 | 2002-04-18 | Microbial silage additive and its preparation method |
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| CN1141884C true CN1141884C (en) | 2004-03-17 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4101729B2 (en) * | 2003-10-22 | 2008-06-18 | 劉 楊 | Livestock feed additive |
| CN101965906A (en) * | 2010-09-15 | 2011-02-09 | 中国热带农业科学院热带作物品种资源研究所 | Preparation method of stylosanthes guianensis biological feed |
| CN102488120B (en) * | 2011-12-28 | 2013-02-13 | 广西东园生态农业科技有限公司 | Preparation method of fermenting ensilaged feed |
| CN105192247A (en) * | 2014-06-25 | 2015-12-30 | 都江堰惠农生物技术有限责任公司 | Green silage additive and preparing method thereof |
| CN106423355B (en) * | 2016-11-30 | 2018-10-02 | 宁夏鑫德粮油工贸有限公司 | A kind of echelon wheat wetting technique |
| CN107439795B (en) * | 2017-06-29 | 2021-02-05 | 青岛海瓴生物科技有限公司 | Feed additive containing composite seaweed and preparation process thereof |
| CN111557378B (en) * | 2020-05-20 | 2022-09-27 | 江南大学 | Method for preparing fermented feed by using bifidobacterium longum |
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2002
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