CN1190145C - Process for producing stalk protein feed by multi-culture united anaerobic fermentation - Google Patents
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Abstract
The present invention relates to a method for producing stalk protein feed by multi-strain united anaerobic fermentation, which belongs to the field of using the multi-strain united anaerobic fermentation for producing the stalk protein feed. The present invention solves the problem of the low resource using rate of the stalks and other crops, and provides the feed increasing the protein content of the stalk feed and the animal foraging rate, and preventing animal intestinal tract diseases. The present invention uses dinitrogen fiber monads, yellow fiber producing monads, bacillus coagulans, lactobacillus acidophilus, bacillus subtilis, starch milk bacillus, complex phosphoesterasum bacteria, bacterium acidi propionici, krusei candida and pseudo endomycetaceae as bacterium sources. The modern biotechnology is used, the fermentation liquid uses vacuum freezing dryness for preparing bacterium powder which is provided with the anaerobic fermentation of crop stalks and other agriculture side products for producing the high protein feed of cattle, sheep, pigs, fish, chicken or other poultries. The present invention provides effective ways for the deep development of the animal husbandry, solving the insufficiency of protein feed, and relieving the conflict of people and animal food competition. Meanwhile, the environmental pollution is reduced, and the present invention has important economical and social benefits.
Description
Technical field
The invention belongs to stalk and other crops accessory substance fermentation production of protein feedstuff technical field, particularly the preparation of many bacterial classifications stover ferment agent and anaerobic fermentation thereof are produced the method for stalk and crops accessory substance protein feed.
Technical background
Along with the fast development of China's animal husbandry, the demand of feed all there has been higher requirement on quantity and kind.Greatly develop be of high nutritive value, feed that production cost is low is the trend of feed industrial development.As everyone knows, China is a large agricultural country, annual agricultural crop straw total output reaches more than 700,000,000 ton, wherein straw is 2.3 hundred million tons, 2.2 hundred million tons of cornstalks, 1.0 hundred million tons of beans and autumn coarse cereals class agricultural crop straws, peanut and potato class tendril, 1.0 hundred million tons of beet tops etc., this is a huge biological available resources.The utilization rate of stalk resource is low at present, only is 33%, and wasting phenomenon is very serious, and the overwhelming majority is directly burned, and causes atmosphere pollution.Arbitrarily stack some areas, and term of office erosion had both caused disaster hidden-trouble, grew mosquitos and flies again, destroys environmental sanitation.The animal husbandry development of China is comparatively slow compared with developed countries, causes the key reason of gap to be feed and feeding manner.The animal husbandry structure of China belongs to " concentrate type " or " feed consumption type " at present, and China has a large population and a few land, and cultivable soil only accounts for 1/7 of whole world cultivated area, but will support the population in the whole world 1/5, and the life of China is very nervous always with grain and grain for industrial uses.Along with the fast development of animal husbandry, the phenomenon of " people and animals strive grain " has appearred.Feed price is high, and the feeding cost height lacks the market competitiveness.The economic profit of livestock breeding industry can't guarantee, has seriously hindered the development of aquaculture.Excavate stalk resource, the development straw feed is the basic outlet that addresses this problem.At present, though carrying out the research unit of the microbial fermentation technology of stalk has much, the processing of concluding to stalk has four kinds substantially, be physical treatment method, chemical method, additive package method and ferment-fermented method, but strictly say, present technology is not perfect, can not push suitability for industrialized production to.The subject matter of prior art is:
(1) some technology only adopts the method for physical treatment, stalk is carried out simple physics handle, and the nutritional labeling of stalk is greatly improved, and the straw utilization rate is low, can not prevent animal intestine stomach trouble well.
(2) some technology only adopts the method for chemistry to handle stalk, utilizes ammoniacal liquor, and the plaster of paris etc. are handled stalk, the cost height, it is low to produce effects, and causes certain pollution, also easily cause the animal food poisoning, also exist the straw utilization rate low, can not prevent the problem of animal intestine stomach trouble well.
(3) some simple employing is added enzyme and various trace elements, and its cost is too high, can't promote well.
(4) lack the bacterial classification that improves forage protein content in some leavening, the stalk protein content of production is low.
(5) the leavening product is single, can only handle a kind of agricultural crop straw, can not satisfy the needs of different stalk kinds and the different object of feeding.
(6) employing that has the method for microorganism ferment making feed, but all under aerobic conditions, ferment, be subjected to harmful contamination by micro inevitably, polluted by aspergillus flavus and happen occasionally, make aspertoxin too high levels in the feed.
Handling the desirable leavening of stalk so release, improve fermented stalk protein feed quality, solve the low problem of straw resource utilization rate, is the task of top priority.
Summary of the invention
At above-mentioned situation, the present invention utilizes modern biotechnology, and the stalk protein feed of handling the high prevention animal intestine stomach trouble of desirable multi-strain fermentation agent of stalk and protein content and animal grazing rate and the method that many bacterial classifications are united, anaerobic fermentation is produced the stalk protein feed thereof are provided; And the corresponding low problem of effective rate of utilization that has solved agricultural straw resource.
Technical scheme of the present invention is achieved in that
The characteristics of the production method of the protein feed of many bacterial classifications anaerobic fermentation agricultural crop straw are: a kind of novel fermentation agent is provided, and its preparation technology adopts five class bacterium, and totally 10 strain bacterium are cultivated respectively; The centrifugal back of zymotic fluid adopts vacuum freeze drying to prepare Mixed Microbes powder pulvis; Agricultural crop straw and part accessory substance are under anaerobic carried out combined ferment, produce protein feed; The present invention specifically may further comprise the steps:
(1) preparation novel straw leavening:
1). select for use following microorganism fungus kind as the bacterium source: dinitrogen cellulomonas cartae A1; Produce yellowish fiber monad A2; Bacillus coagulans B1; Acidophilus strain B 2; Bacillus subtilis C1; Starch milk bacillus C2; Composite enzyme bacterium D1; Bacterium acidi propionici D2; The comparable Candida E1 of youth; Intend endomyces E2.
2). the preparation technology of stover ferment agent is as follows:
Slant strains → liquid spawn → level liquid cultivation → secondary Liquid Culture → three grades of Liquid Culture → ferment tank → centrifugal → vacuum freeze drying → pulverizing → generals: dinitrogen cellulomonas cartae A1; Produce yellowish fiber monad A2; Bacillus coagulans B1; Acidophilus strain B 2; Bacillus subtilis C1; Starch milk bacillus C2; Composite enzyme bacterium D1; Bacterium acidi propionici D2; The comparable Candida E1 of youth; Intend ten kinds of bacterium powder of endomyces E2 and mix → make many bacterial classifications stover ferment agent.Its concrete preparation technology is as follows:
(1) slant strains: dinitrogen cellulomonas cartae A1; Produce yellowish fiber monad A2; The inclined-plane is preserved and is used straw powder agar medium, and Liquid Culture is used peptone 1%, glucose 1%, yeast extract 0.5%, the culture medium of pH value 7.0 with fermentation; Yeast extract 1%, peptone 1%, glucose 0.6%, dipotassium hydrogen phosphate 0.2%, the culture medium of magnesium sulfate 0.1% are used in bacillus coagulans B1 inclined-plane preservation and Liquid Culture and fermentation; Acidophilus strain B 2 bacterial classifications are preserved and are used 10% milk powder culture medium, and Liquid Culture is used beef extract 1%, peptone 1%, glucose 2%, yeast extract 0.5%, the culture medium of milk powder 1% with fermentation; Bacillus subtilis C1; Starch milk bacillus C2; Broth bouillon is all used in composite enzyme bacterium D1 inclined-plane preservation and Liquid Culture and fermentation; Peptone 1% is all used in bacterium acidi propionici D2 inclined-plane preservation and Liquid Culture and fermentation, yeast extract 1%, sucrose 2%, the culture medium of ammonium phosphate 0.5%; The comparable Candida E1 of youth; Intend endomyces E2 inclined-plane and preserve use 10% malt extract medium, Liquid Culture is used peptone 2%, yeast extract, glucose 2% with fermentation; The static cultivation under 28～30 ℃ of conditions of all slant strains was put into 4 ℃ of refrigerators and is preserved after 20～24 hours.
(2) liquid spawn and level liquid are cultivated: picking 2 encircles in corresponding culture medium, and shaking table was cultivated 20～24 hours under 28～30 ℃ of conditions; Carry out inoculum concentration again and be 5～10% inoculation in corresponding culture medium, shaking table was cultivated 20～24 hours under 28～30 ℃ of conditions, carried out level liquid and cultivated.
(3) secondary Liquid Culture: carry out inoculum concentration and be 5～10% inoculation in corresponding culture medium, shaking table was cultivated 20～24 hours under 28～30 ℃ of conditions, carried out the secondary Liquid Culture.
(4) three grades of Liquid Culture: carry out inoculum concentration and be 5～10% inoculation in corresponding culture medium, shaking table was cultivated 20～24 hours under 28～30 ℃ of conditions, carried out three grades of Liquid Culture.
(5) ferment tank: carry out inoculum concentration and be 5～10% inoculation in corresponding culture medium, shaking table was cultivated 20～24 hours under 28～30 ℃ of conditions, carried out the Liquid Culture fermentation.
(6) centrifugal, vacuum freeze drying and pulverizing: zymotic fluid is directly imported centrifuge carries out centrifugal back and in advancing vacuum freeze drier, handle, to final water content 3%.Pulverize then.
(7) mix: above-mentioned ten kinds of bacterium powder are mixed, be leavening.
(2) utilize many bacterial classifications anaerobic fermentation to produce stalk and other agricultural and sideline product albumen feed:
Agricultural crop straw and other agricultural and sideline product are cut up with a hay cutter the powder of straw that is swaged into 3～5cm or is ground into 20 sieve meshes, each ton adds the leavening 3g that a bag mixes, the mixed proportion of agricultural crop straw and water is 1: 0.5～1, after mixing, under 7～40 ℃ of anaerobic conditions, ferment, can make protein content in 35～7 days and be 10～30% the protein feed that is applicable to ox, sheep, pig, fish, chicken or other poultry, wherein the fish protein feed is a graininess.
Need to prove: the production method of the protein feed of many bacterial classifications anaerobic fermentation agricultural crop straw, according to the different objects of feeding, different raw material and agricultural and sideline product thereof, ratio that can each bacterial classification of appropriate change prepares the different leavening that meets production requirement.Dinitrogen cellulomonas cartae A1 in the stover ferment agent; Produce yellowish fiber monad A2; Bacillus coagulans B1; Acidophilus strain B 2; Bacillus subtilis C1; Starch milk bacillus C2; Composite enzyme bacterium D1; Bacterium acidi propionici D2; The comparable Candida E1 of youth; The mixed proportion of intending endomyces E2 is preferably: 1.5～2: 1.5～2: 0.5～1.5: 0.5～1.5: 0.5～1.5: 0.5～1.5: 0.5～1.5: 0.5～1.5: 1.5～2: 1.5～2.
The principle of the invention is to utilize many bacterial classifications anaerobic fermentation to produce the method for stalk protein feed, and the preparation technology of this leavening adopts five class bacterium, and totally 10 strain bacterium are cultivated respectively, and the centrifugal back of zymotic fluid adopts vacuum freeze drying to prepare Mixed Microbes powder pulvis.Since in this leavening special assembly acid-producing bacteria, in anaerobic fermentation process, it is enough low that the lactic acid of generation, propionic acid etc. drop to the pH value of feed, suppressed growing of pernicious bacteria effectively, guaranteed the security of feed and be easy to keeping quality.
Beneficial effect:
Product of the present invention is that agricultural crop straw and other agricultural and sideline product under anaerobic carry out the series high protein feed that combined ferment is produced.Its method makes full use of microbial fermentation technology, according to the physio-biochemical characteristics of different strain, carries out the optimum organization of bacterial classification, and the inoculating proportion in the appropriate change leavening can obtain meeting the serial leavening of production requirement.But this leavening useful effect is in various agricultural crop straws and the agricultural and sideline product of part.Technology of the present invention is according to the different objects of feeding, and different stalks and agricultural and sideline product can be made into bovine protein feed, sheep protein feed, pig protein feedstuff, fish protein feed (making particle), the white feed of egg or other poultry protein feed.The present invention rely on microbial reproduction fast, easily cultivate, characteristic that metabolic capability is strong, utilize the technology of many bacterial classifications anaerobic fermentation, produce various stalk protein feeds, overcome the drawback of prior art, reached the protein content that improves straw feed, the purpose that improves the effective rate of utilization of animal grazing rate, prevention animal intestine stomach trouble, raising stalk.Since in this leavening special assembly acid-producing bacteria, in anaerobic fermentation process, it is enough low that the lactic acid of generation, propionic acid etc. drop to the pH value of feed, suppressed growing of pernicious bacteria effectively, guaranteed the security of feed and be easy to keeping quality.Major advantage of the present invention is summarized as follows:
1. low, the small investment of production cost, instant effect, high efficiency;
2. the present invention adopts Vacuum Freezing ﹠ Drying Technology to carry out the processing of zymotic fluid, can keep the activity and the number in bacterium source well.
3. practical, easy to operate, easily promote;
4. fermentation condition is extensive, and the cycle is short, the production efficiency height;
5. non-secondary pollution is beneficial to environmental protection;
6. with this ferment-fermented stalk protein feed that makes, its protein content height, L-lactic acid content height is rich in profitable strain, can obviously improve the output of milk and the butterfat percnetage of milk cow after edible, helps the zooprophylazis enterogastritis.
7. overcome the disadvantage that existing product is single, can not satisfy different stalk kinds and the different object of feeding, provide series to meet the leavening of production requirement and satisfy the series high protein feed of many-sided demand, wherein the fish protein feed is made graininess; Be widely applicable for the nursing of ox, sheep, pig, fish, chicken or other poultry.
In this leavening special assembly acid-producing bacteria, in anaerobic fermentation process, it is enough low that the lactic acid of generation, propionic acid etc. drop to the pH value of feed, suppressed growing of pernicious bacteria effectively, guaranteed the security of feed and be easy to keeping quality.
The present invention for the deficiency of greatly developing, solve protein feed of animal husbandry, alleviate contradiction that people and animals strive grain, improve agroecological environment, reduce environmental pollution, promote the effect that agricultural structure is adjusted, effective way is provided, and has realized making full use of of resource.Have great economic benefit and social benefit.
The specific embodiment
Embodiment 1:
A kind ofly utilize the method for crop straws for producing protein feed by many bacterial classifications anaerobic fermentation, it may further comprise the steps:
(1) preparation stover ferment agent:
1) select for use following microorganism fungus kind as the bacterium source: dinitrogen cellulomonas cartae A1; Produce yellowish fiber monad A2; Bacillus coagulans B1; Acidophilus strain B 2; Bacillus subtilis C1; Starch milk bacillus C2; Composite enzyme bacterium D1; Bacterium acidi propionici D2; The comparable Candida E1 of youth; Intend endomyces E2;
2) preparation technology of stover ferment agent is as follows:
Slant strains → liquid spawn → level liquid cultivation → secondary Liquid Culture → three grades Liquid Culture → ferment tank → centrifugal → vacuum freeze drying → pulverizing → the tunning of ten kinds of bacterial classifications such as A1, A2, B1, B2, C1, C2, D1, D2, E1, E2 is mixed → make different stover ferment agents in different ratios;
Below preparation technology is described in detail:
(1) slant strains: A1, A2 inclined-plane are preserved and are used straw powder agar medium, and Liquid Culture is used peptone 1%, glucose 1%, yeast extract 0.5%, the culture medium of pH value 7.0 with fermentation; Preservation of B1 inclined-plane and Liquid Culture and fermentation are same: use yeast extract 1%, peptone 1%, glucose 0.6%, dipotassium hydrogen phosphate 0.2%, the culture medium of magnesium sulfate 0.1%; The B2 bacterial classification is preserved and is used 10% milk powder culture medium, and Liquid Culture is used beef extract 1%, peptone 1%, glucose 2%, yeast extract 0.5%, the culture medium of milk powder 1% with fermentation; Broth bouillon is all used in C1, C2, the preservation of D1 inclined-plane and Liquid Culture and fermentation; Peptone 1% is all used in preservation of D2 inclined-plane and Liquid Culture and fermentation, yeast extract 1%, sucrose 2%, the culture medium of ammonium phosphate 0.5%; E1, E2 inclined-plane are preserved and are used 10% malt extract medium, and Liquid Culture is used peptone 2%, yeast extract, glucose 2% with fermentation; The static cultivation under 28～30 ℃ of conditions of all slant strains was put into 4 ℃ of refrigerators and is preserved after 24 hours;
(2) liquid spawn and level liquid are cultivated: picking 2 encircles in corresponding culture medium, cultivates 24 hours under 28～30 ℃ of conditions; In corresponding culture medium, carry out inoculum concentration again and be 5～10% inoculation, under 28～30 ℃ of conditions, cultivated 24 hours, carry out level liquid and cultivate;
(3) secondary Liquid Culture: in corresponding culture medium, carry out inoculum concentration and be 5～10% inoculation, under 28～30 ℃ of conditions, cultivated 24 hours, carry out the secondary Liquid Culture;
(4) three grades of Liquid Culture: in corresponding culture medium, carry out inoculum concentration and be 5～10% inoculation, under 28～30 ℃ of conditions, cultivated 24 hours, carry out three grades of Liquid Culture;
(5) ferment tank: in corresponding culture medium, carry out inoculum concentration and be 5～10% inoculation, under 28～30 ℃ of conditions, cultivated 24 hours, carry out the Liquid Culture fermentation;
(6) centrifugal, vacuum freeze drying and pulverizing: zymotic fluid is directly imported centrifuge in vacuum freeze drier, handles again after centrifugal, to final water content 3%.Pulverize then;
(7) mix: above-mentioned ten kinds of bacterium powder are mixed.With dinitrogen cellulomonas cartae A1, produce yellowish fiber monad A2, bacillus coagulans B1, acidophilus strain B 2, bacillus subtilis C1, starch milk bacillus C2, composite enzyme bacterium D1, bacterium acidi propionici D2, the comparable Candida E1 of youth, intend endomyces E2 by following ratio 2: 2: 1: 1: 1: 1.5: 1.5: 1: 2: 2 mixed must the stover ferment agent products.
(2) utilize many bacterial classifications anaerobic fermentation to produce the stalk protein feed:
Agricultural crop straw is got cornstalk, and hand hay cutter is swaged into 3～5cm, and each ton adds the leavening 3g that a bag mixes, and the mixed proportion of crops cornstalk and water is 1: 0.5～1, after mixing, under anaerobic ferments.Under 10 ℃ of conditions, can make protein content in 30 days and be 15～20% stalk protein feed.It is feeding that this cornstalk protein feed can be supplied with ruminants such as cattle and sheep.
Embodiment 2:
Preparation stover ferment agent and stalk are handled with embodiment 1; Need to prove, utilize many bacterial classifications anaerobic fermentation to produce the temperature of stalk protein feed, is under 35 ℃ condition, can make protein content in 7 days and be 15～20% stalk protein feed.It is feeding that this stalk protein feed can be supplied with ruminants such as cattle and sheep.
Embodiment 3:
Preparation stover ferment agent and stalk are handled with embodiment 1; Need to prove, utilize many bacterial classifications anaerobic fermentation to produce the temperature of stalk protein feed, is under 20 ℃ condition, can make protein content in 20 days and be 20～30% stalk protein feed.It is feeding that this stalk protein feed can be supplied with ruminants such as cattle and sheep.
Embodiment 4:
Stover ferment agent is with dinitrogen cellulomonas cartae A1, produce yellowish fiber monad A2, bacillus coagulans B1, acidophilus strain B 2, bacillus subtilis C1, starch milk bacillus C2, composite enzyme bacterium D1, bacterium acidi propionici D2, the comparable Candida E1 of youth, intend endomyces E2 in following ratio 1.5: 1.5: 1.5: 1.5: 1.5: 1: 0.5: 0.5: 1.5: 1.5 were mixed, and made the stalk mixed culture fermentation agent.Agricultural crop straw is got straw, is ground into the powder of straw of 20 sieve meshes.The mixed proportion of rice straw powder and water and other technology, method are with embodiment 1.It is feeding that this rice straw protein feed can be supplied with non-ruminant animals such as pig chicken.
Embodiment 5:
Preparation stover ferment agent and stalk are handled with embodiment 4; Other technology, method are with embodiment 2.It is feeding that this rice straw protein feed can be supplied with non-ruminant animals such as pig chicken.
Embodiment 6:
Preparation stover ferment agent and stalk are handled with embodiment 4; Other technology, method are with embodiment 3.It is feeding that this rice straw protein feed can be supplied with non-ruminant animals such as pig chicken.
Embodiment 7:
Stover ferment agent is with dinitrogen cellulomonas cartae A1, produce yellowish fiber monad A2, bacillus coagulans B1, acidophilus strain B 2, bacillus subtilis C1, starch milk bacillus C2, composite enzyme bacterium D1, bacterium acidi propionici D2, the comparable Candida E1 of youth, intend endomyces E2 in following ratio 1.8: 1.8: 0.5: 0.5: 0.5: 0.5: 1: 1.5: 1.8: 1.8 were mixed, and make mixed culture fermentation agent.The crops accessory substance is got bagasse, is ground into the ground-slag of 20 sieve meshes.The mixed proportion of bagasse powder and water and other technology, method are with embodiment 1.This bagasse powder protein feed, it is feeding to supply with non-ruminant animals such as pig chicken.
Embodiment 8:
Stover ferment agent is with dinitrogen cellulomonas cartae A1, produce yellowish fiber monad A2, bacillus coagulans B1, acidophilus strain B 2, bacillus subtilis C1, starch milk bacillus C2, composite enzyme bacterium D1, bacterium acidi propionici D2, the comparable Candida E1 of youth, intend endomyces E2 bacterium powder in following ratio 1.5: 1.5: 1.5: 1.5: 1.5: 1: 0.5: 0.5: 2: 2 mixings made the stalk mixed culture fermentation agent.Agricultural crop straw is got straw, is ground into the powder of straw of 20 sieve meshes.The mixed proportion of rice straw powder and water and other technology, method are with embodiment 1.Fermented behind the feed through the expanded particle of making.It is feeding that this protein feed can be supplied with fish.
Claims (2)
1. utilize many bacterial classifications anaerobic fermentation to produce the method for stalk protein feed, it is characterized in that: adopt novel multi-strain fermentation agent, the preparation technology of this leavening adopts five class bacterium, and totally 10 strain bacterium are cultivated respectively; The centrifugal back of zymotic fluid adopts vacuum freeze drying to prepare bacterium powder pulvis; Agricultural crop straw and part accessory substance are under anaerobic carried out combined ferment, produce protein feed; Specifically may further comprise the steps:
(1) preparation novel straw leavening:
1) select for use following microorganism fungus kind as the bacterium source: dinitrogen cellulomonas cartae (A1); Produce yellowish fiber monad (A2); Bacillus coagulans (B1); Lactobacillus acidophilus (B2); Bacillus subtilis (C1); Starch milk bacillus (C2); Composite enzyme bacterium (D1); Bacterium acidi propionici (D2); The comparable Candida of youth (E1); Intend endomyces (E2);
2) preparation technology of stover ferment agent is as follows:
Slant strains → liquid spawn → level liquid cultivation → secondary Liquid Culture → three grades of Liquid Culture → ferment tank → centrifugal → vacuum freeze drying → pulverizing → generals: dinitrogen cellulomonas cartae (A1); Produce yellowish fiber monad (A2); Bacillus coagulans (B1); Lactobacillus acidophilus (B2); Bacillus subtilis (C1); Starch milk bacillus (C2); Composite enzyme bacterium (D1); Bacterium acidi propionici (D2); The comparable Candida of youth (E1); Intend ten kinds of bacterium powder of endomyces (E2) and mix → make many bacterial classifications stover ferment agent; Its concrete preparation technology is as follows:
(1) slant strains: dinitrogen cellulomonas cartae (A1); Produce yellowish fiber monad (A2); The inclined-plane is preserved and is used straw powder agar medium, and Liquid Culture is used peptone 1%, glucose 1%, yeast extract 0.5%, the culture medium of pH value 7.0 with fermentation; Yeast extract 1%, peptone 1%, glucose 0.6%, dipotassium hydrogen phosphate 0.2%, the culture medium of magnesium sulfate 0.1% are used in bacillus coagulans (B1) inclined-plane preservation and Liquid Culture and fermentation; Lactobacillus acidophilus (B2) bacterial classification is preserved and is used 10% milk powder culture medium, and Liquid Culture is used beef extract 1%, peptone 1%, glucose 2%, yeast extract 0.5%, the culture medium of milk powder 1% with fermentation; Bacillus subtilis (C1); Starch milk bacillus (C2); Broth bouillon is all used in composite enzyme bacterium (D1) inclined-plane preservation and Liquid Culture and fermentation; Peptone 1% is all used in bacterium acidi propionici (D2) inclined-plane preservation and Liquid Culture and fermentation, yeast extract 1%, sucrose 2%, the culture medium of ammonium phosphate 0.5%; The comparable Candida of youth (E1); Intend endomyces (E2) inclined-plane and preserve use 10% malt extract medium, Liquid Culture is used peptone 2%, yeast extract, glucose 2% with fermentation; The static cultivation under 28～30 ℃ of conditions of all slant strains was put into 4 ℃ of refrigerators and is preserved after 20～24 hours.
(2) liquid spawn and level liquid are cultivated: picking 2 encircles in corresponding culture medium, and shaking table was cultivated 20～24 hours under 28～30 ℃ of conditions; Carry out inoculum concentration again and be 5～10% inoculation in corresponding culture medium, shaking table was cultivated 20～24 hours under 28～30 ℃ of conditions, carried out level liquid and cultivated;
(3) secondary Liquid Culture: carry out inoculum concentration and be 5～10% inoculation in corresponding culture medium, shaking table was cultivated 20～24 hours under 28～30 ℃ of conditions, carried out the secondary Liquid Culture;
(4) three grades of Liquid Culture: carry out inoculum concentration and be 5～10% inoculation in corresponding culture medium, shaking table was cultivated 20～24 hours under 28～30 ℃ of conditions, carried out three grades of Liquid Culture;
(5) ferment tank: carry out inoculum concentration and be 5～10% inoculation in corresponding culture medium, shaking table was cultivated 20～24 hours under 28～30 ℃ of conditions, carried out the Liquid Culture fermentation;
(6) centrifugal, vacuum freeze drying and pulverizing: zymotic fluid is directly imported centrifuge carries out centrifugal back and in advancing vacuum freeze drier, handle, to final water content 3%.Pulverize then;
(7) mix: above-mentioned ten kinds of bacterium powder are mixed, be leavening;
(2) utilize many bacterial classifications anaerobic fermentation to produce stalk and other agricultural and sideline product albumen feed: agricultural crop straw and other agricultural and sideline product are cut up with a hay cutter the powder of straw that is swaged into 3～5cm or is ground into 20 sieve meshes, each ton adds the leavening 3g that a bag mixes, the mixed proportion of agricultural crop straw and water is 1: 0.5～1, after mixing, under 7～40 ℃ of anaerobic conditions, ferment, can make protein content in 35～7 days and be 10～30% the protein feed that is applicable to ox, sheep, pig, fish, chicken or other poultry, wherein the fish protein feed is a graininess.
2. according to the described method of utilizing many bacterial classifications anaerobic fermentation to produce the stalk protein feed of claim 1, it is characterized in that: according to the different objects of feeding, different raw material and agricultural and sideline product thereof, the ratio that changes each bacterial classification prepares the different leavening that meets production requirement, dinitrogen cellulomonas cartae (A1) in the stover ferment agent; Produce yellowish fiber monad (A2); Bacillus coagulans (B1); Lactobacillus acidophilus (B2); Bacillus subtilis (C1); Starch milk bacillus (C2); Composite enzyme bacterium (D1); Bacterium acidi propionici (D2); The comparable Candida of youth (E1); The mixed proportion of intending endomyces (E2) is preferably: 1.5～2: 1.5～2: 0.5～1.5: 0.5～1.5: 0.5～1.5: 0.5～1.5: 0.5～1.5: 0.5～1.5: 1.5～2: 1.5～2.
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| CN101138392B (en) * | 2007-07-30 | 2011-08-17 | 湖南农业大学 | Micro-zoology preparations for feeding |
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| CN102349594B (en) * | 2011-09-29 | 2013-01-23 | 湘潭大学 | Method for carrying out comprehensive utilization on fish guts |
| CN104543336A (en) * | 2014-12-25 | 2015-04-29 | 山东西王糖业有限公司 | Method for producing feed through pretreating at high temperature and fermenting at low temperature |
| CN105104857A (en) * | 2015-08-28 | 2015-12-02 | 方标嵩 | Fermented feed for feeding wild porkers and production method thereof |
| CN105475643A (en) * | 2015-12-25 | 2016-04-13 | 广州甘蔗糖业研究所 | Sugar cane byproduct cattle feed capable of promoting digestion |
| CN105586295A (en) * | 2016-01-22 | 2016-05-18 | 竹山县鑫源皂素有限责任公司 | Bacillus coagulans, culture method thereof and application of Bacillus coagulans in preparation of bio-organic fertilizer |
| CN107629982A (en) * | 2017-10-17 | 2018-01-26 | 高俊 | A kind of preparation method of efficient straw leavening |
| CN108048346B (en) * | 2017-12-06 | 2021-06-18 | 辽宁省农业科学院 | Compound microbial inoculum for morchella cultivation and external nutrition and application thereof |
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2003
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103229902A (en) * | 2013-03-29 | 2013-08-07 | 蚌埠大成食品有限公司 | High-protein cattle forage and preparation method thereof |
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