CN100417335C - Composite life beneficial bacteria culture for improving milk cow production performance and its making method - Google Patents
Composite life beneficial bacteria culture for improving milk cow production performance and its making method Download PDFInfo
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- CN100417335C CN100417335C CNB2005100099499A CN200510009949A CN100417335C CN 100417335 C CN100417335 C CN 100417335C CN B2005100099499 A CNB2005100099499 A CN B2005100099499A CN 200510009949 A CN200510009949 A CN 200510009949A CN 100417335 C CN100417335 C CN 100417335C
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- saccharomycete
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Abstract
The present invention provides a compound probiotic culture for improving the production performance of a milk cow, which is one of the following saccharomycetes including saccharomycopsis fibuligera, saccharomyce cerevisiae or Candida utilis, and Bulgarian acidophilic bacterium lacticum and lactobacillus. The compound probiotic culture is obtained in a processing mode by a solid and liquid mixing fermentation method, a liquid culture inoculum size enlarging method, etc. The compound probiotic culture is a novel green feed additive for a high-yield milk cow. If 50g of the compound probiotic culture is added into fine feed for each milk cow every day, the phenomenon that the milk yield of the high-yield milk cow is lowered under the condition of heat stress at the prior period of lactation can be can effectively relieved. A feeding experiment discovers that the milk yield of a group in which the compound probiotic culture is added is raised by 6.5% as compared with a contrast group. The compound probiotic culture is mainly suitable for the high-yield milk cow at the prior period of lactation, and has obvious effects under the condition of the heat stress in summer.
Description
(1) technical field
What the present invention relates to is a kind of feed addictive, specifically a kind of feed addictive that improves ruminant animal nutrition.The present invention also relates to the preparation method of this feed addictive.
(2) background technology
For high yield cow, descend in lactation feed intake in early stage, the energy of taking in from daily ration can not satisfy the needs of keeping with high yield, the produce power negative balance, milk cow is employed body fat in order to keep the output of milk, causes body weight to descend.Too many loss of weight can produce adverse influence to the whole lactational performance of giving milk, and can prolong the breeding time of milk cow simultaneously, and that has reduced milk cow effectively utilizes the time limit.Under the single relatively situation of present roughage, the concentrated feed amount is taked to increase in most places increases the output of milk, and the result produces multiple metabolic disease, and the benefit of milk cattle cultivating is reduced.How having taken good care of the cud of milk cow, made the little ecosystem in the cow rumen reach optimum state, improve the output of milk under the situation that does not change the daily ration structure, is the focus of studying both at home and abroad now.
(3) summary of the invention
The object of the present invention is to provide a kind of cud that can take good care of milk cow, make the little ecosystem in the cow rumen reach optimum state, under the situation that does not change the daily ration structure, can improve the compound probiotic culture that improves milk cow production performance of the output of milk.The present invention also aims to provide the processing method of this compound probiotic culture.
The object of the present invention is achieved like this: it is saccharomycete and Bulgarian lactobacillus acidophilus, adopts following method to process resulting product:
1, each rejuvenation of spawn: use aseptic technique to change in the test tube that sterilized solid medium is housed the barms of preserving, use aseptic technique to change in the conical flask that sterilized fluid nutrient medium is housed the Bulgarian lactobacillus acidophilus kind of preserving, at 30 ℃ of constant temperature culture 3-5 days;
2, saccharomycete bacteria suspension preparation: inject the SPSS of the 1/4-1/3 that accounts for the test tube volume in cultivating the saccharomycete test tube that finishes, vibration is washed, up to the physiological saline muddiness;
3, saccharomycete liquid medium preparation: with adding 3 times of water, 65 ℃ of water-bath saccharification after the fructus hordei germinatus pulverizing, filter, regulate pol and pH value and be respectively 15 ripple woodss, 6, under 121 ℃ of temperature, sterilized 20 minutes then;
4, saccharomycete liquid is cultivated: add the bacteria suspension for preparing in being cooled to 28-32 ℃ fluid nutrient medium, 30 ℃ of water-baths are cultivated, and stop when the fluid nutrient medium of clarification is obviously muddy;
5, compound bacteria solid medium preparation: is 82: 7: 11.8 with wheat bran, corn flour, dregs of beans and urea according to weight ratio: 0.2 ratio is mixed, and the adding weight ratio is 60% water, evenly mixes back 121 ℃ of sterilizations;
6, cultivate: cultured saccharomycete liquid culture medium and Bulgarian lactobacillus acidophilus fluid nutrient medium are admixed in the sterilized solid medium, inoculum concentration is 10%, and wherein the ratio of two kinds of bacterium is 2: 1, puts into tray and cultivates, 30 ℃ of temperature, time 72h;
7, drying: low temperature drying after the fermentation ends, packing.
Product of the present invention is to adopt such method to make:
1, each rejuvenation of spawn: use aseptic technique to change in the test tube that sterilized solid medium is housed the barms of preserving, use aseptic technique to change in the conical flask that sterilized fluid nutrient medium is housed the Bulgarian lactobacillus acidophilus kind of preserving, at 30 ℃ of constant temperature culture 3-5 days;
2, saccharomycete bacteria suspension preparation: inject the SPSS of the 1/4-1/3 that accounts for the test tube volume in cultivating the saccharomycete test tube that finishes, vibration is washed, up to the physiological saline muddiness;
3, saccharomycete liquid medium preparation: with adding 3 times of water, 65 ℃ of water-bath saccharification after the fructus hordei germinatus pulverizing, filter, regulate pol and pH value and be respectively 15 ripple woodss, 6, under 121 ℃ of temperature, sterilized 20 minutes then;
4, saccharomycete liquid is cultivated: add the bacteria suspension for preparing in being cooled to 28-32 ℃ fluid nutrient medium, 30 ℃ of water-baths are cultivated, and stop when the fluid nutrient medium of clarification is obviously muddy;
5, compound bacteria solid medium preparation: is 82: 7: 11.8 with wheat bran, corn flour, dregs of beans and urea according to weight ratio: 0.2 ratio is mixed, and the adding weight ratio is 60% water, evenly mixes back 121 ℃ of sterilizations;
6, cultivate: cultured saccharomycete liquid culture medium and Bulgarian lactobacillus acidophilus fluid nutrient medium are admixed in the sterilized solid medium, inoculum concentration is 10%, and wherein the ratio of two kinds of bacterium is 2: 1, puts into tray and cultivates, 30 ℃ of temperature, time 72h;
7, drying: low temperature drying after the fermentation ends, packing are made product.
Method of the present invention can also comprise:
1, described saccharomycete is a saccharomycopsis fibuligera.
2, described saccharomycete is a saccharomyces cerevisiae.
3, described saccharomycete is a candida utili.
The present invention adopts the solid-liquid composting fermentation by the screening to bacterial classification, strengthens the technological means such as inoculum concentration of liquid culture.By screening to different bacterium, pick out the improved seeds of under China's present stage raising condition (sheep's hay+fine fodder), utilize the discarded object of agricultural production to ferment under certain conditions through specially treated and form product as raw material.This product mainly is by changing the lumen fermentation type, increase the capacity usage ratio of animal to roughage, finally reaching the purpose of volume increase.That product has is cheap, use characteristics such as simple, that addition is few, additive effect is obvious, have no adverse reaction.According to evidence, add 50g product of the present invention, can improve output of milk 1.75Kg, if the milk price of butterfat percnetage 3.5% is 1.8 yuan/Kg, then input-output ratio is 1: 26.
Product of the present invention is a kind of a kind of novel green feed additive of high yield cow, every ox is added 50g every day in fine fodder, can effectively extenuate the output of milk that the high yield cow lactation is under the heat stress condition in earlier stage and descend, find that by feeding experiment the interpolation group improves the output of milk 6.5% than control group.The main applicable object of this product is the high yield cow in lactation early stage, and effect is especially obvious under summer fever stress situation.
The color and luster taupe of product of the present invention is mixed with certain white powder material, and sour milk fragrance is arranged, and product is mainly regulated and control cow rumen by dual mode, and the firstth, the living cells that contains in the product, the living cells quantity in this product can reach 5,500,000,000 cfu/g; The secondth, the active factors that contains in the product.By the two acting in conjunction the little ecology of cow rumen is regulated, thereby improve the output of milk.
(4) specific embodiments
For example the present invention is done more detailed description below:
The saccharomycete name that this product uses is called any of saccharomycopsis fibuligera, saccharomyces cerevisiae and candida utili kind, the lactic acid bacteria name is called Bulgarian lactobacillus acidophilus, used bacterial classification different from the past, for guaranteeing product quality, adopt the solid-liquid composting fermentation, strengthen the inoculum concentration of liquid culture.The concrete operations step is as follows:
1 each rejuvenation of spawn uses aseptic technique to change in the test tube that sterilized solid medium is housed the barms of preserving, use aseptic technique to change in the conical flask that sterilized fluid nutrient medium is housed the lactobacillus inoculation of preserving, at 30 ℃ of constant temperature culture 3-5 days.
The preparation of 2 saccharomycete bacteria suspensions is injected SPSS in cultivating the saccharomycete test tube that finishes, be approximately 1/4 of test tube volume, and vibration is washed, up to the physiological saline muddiness.
3 saccharomycete liquid medium preparation fructus hordei germinatus add water, water-bath saccharification after pulverizing, and filter, and regulate pol and pH value and are respectively 15 ripple woodss, 6, sterilize 20 minutes for 121 ℃ then.
4 saccharomycete liquid are cultivated and add the bacteria suspension for preparing in the fluid nutrient mediums that is cooled to about 30 ℃, and 30 ℃ of water-baths are cultivated,, when the fluid nutrient medium of clarifying is obviously muddy, stop, needing 20h.
The preparation of 5 compound bacteria solid mediums is 82: 7: 11.8 with wheat bran, corn flour, dregs of beans and urea according to weight ratio: 0.2 ratio is mixed, and adds 60% water, evenly mixes back 121 ℃ of sterilizations.
6 cultivations are admixed cultured saccharomycete liquid culture medium and lactic acid bacteria liquid culture medium in the sterilized solid medium, and inoculum concentration is 10%, puts into tray and cultivates, 30 ℃ of temperature, time 72h.
Low temperature drying after the 7 dry fermentation ends, packing get final product.
Claims (5)
1. compound probiotic culture that improves milk cow production performance, it is characterized in that: it is saccharomycete and Bulgarian lactobacillus acidophilus, adopts following method to process resulting product:
(1), each rejuvenation of spawn: use aseptic technique to change in the test tube that sterilized solid medium is housed the barms of preserving, use aseptic technique to change in the conical flask that sterilized fluid nutrient medium is housed the Bulgarian lactobacillus acidophilus kind of preserving, at 30 ℃ of constant temperature culture 3-5 days;
(2), saccharomycete bacteria suspension preparation: inject the SPSS of the 1/4-1/3 that accounts for the test tube volume in cultivating the saccharomycete test tube that finishes, vibration is washed, up to the physiological saline muddiness;
(3), saccharomycete liquid medium preparation: with adding 3 times of water, 65 ℃ of water-bath saccharification after the fructus hordei germinatus pulverizing, filter, regulate pol and pH value and be respectively 15 ripple woodss, 6, under 121 ℃ of temperature, sterilized 20 minutes then;
(4), saccharomycete liquid cultivates: add the bacteria suspension for preparing in being cooled to 28-32 ℃ fluid nutrient medium, 30 ℃ of water-baths are cultivated, and stop when the fluid nutrient medium of clarification is obviously muddy;
(5), compound bacteria solid medium preparation: with wheat bran, corn flour, dregs of beans and urea according to 82: 7: 11.8: 0.2 ratio is mixed, and adds weight ratio and be 60% water, evenly mixes back 121 ℃ of sterilizations;
(6), cultivate: cultured saccharomycete liquid culture medium and Bulgarian lactobacillus acidophilus fluid nutrient medium are admixed in the sterilized solid medium, inoculum concentration is 10%, and wherein the ratio of two kinds of bacterium is 2: 1, puts into tray and cultivates, 30 ℃ of temperature, time 72h;
(7), drying: low temperature drying after the fermentation ends, packing.
2. preparation method of improving the compound probiotic culture of milk cow production performance is characterized in that:
(1) each rejuvenation of spawn: use aseptic technique to change in the test tube that sterilized solid medium is housed the barms of preserving, use aseptic technique to change in the conical flask that sterilized fluid nutrient medium is housed the Bulgarian lactobacillus acidophilus kind of preserving, at 30 ℃ of constant temperature culture 3-5 days;
(2) saccharomycete bacteria suspension preparation: inject the SPSS of the 1/4-1/3 that accounts for the test tube volume in cultivating the saccharomycete test tube that finishes, vibration is washed, up to the physiological saline muddiness;
(3) saccharomycete liquid medium preparation: with adding 3 times of water, 65 ℃ of water-bath saccharification after the fructus hordei germinatus pulverizing, filter, regulate pol and pH value and be respectively 15 ripple woodss, 6, under 121 ℃ of temperature, sterilized 20 minutes then;
(4) saccharomycete liquid is cultivated: add the bacteria suspension for preparing in being cooled to 28-32 ℃ fluid nutrient medium, 30 ℃ of water-baths are cultivated, and stop when the fluid nutrient medium of clarification is obviously muddy;
(5) compound bacteria solid medium preparation: with wheat bran, corn flour, dregs of beans and urea according to 82: 7: 11.8: 0.2 ratio is mixed, and adds weight ratio and be 60% water, evenly mixes back 121 ℃ of sterilizations;
(6) cultivate: cultured saccharomycete liquid culture medium and Bulgarian lactobacillus acidophilus fluid nutrient medium are admixed in the sterilized solid medium, inoculum concentration is 10%, and wherein the ratio of two kinds of bacterium is 2: 1, puts into tray and cultivates, 30 ℃ of temperature, time 72h;
(7) drying: low temperature drying after the fermentation ends, packing are made product.
3. the preparation method of improving the compound probiotic culture of milk cow production performance according to claim 2, it is characterized in that: described saccharomycete is a saccharomycopsis fibuligera.
4. the preparation method of improving the compound probiotic culture of milk cow production performance according to claim 2, it is characterized in that: described saccharomycete is a saccharomyces cerevisiae.
5. the preparation method of improving the compound probiotic culture of milk cow production performance according to claim 2, it is characterized in that: described saccharomycete is a candida utili.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283321A (en) * | 2010-06-18 | 2011-12-21 | 哈尔滨爱特杰牧业有限公司 | Cow rumen fermentation accelerator |
Families Citing this family (7)
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| CN101313732B (en) * | 2008-07-17 | 2011-09-21 | 潘玉林 | Semi-wet livestock and poultry fine ecological feed additive agent and preparation method thereof |
| CN101637220B (en) * | 2008-07-29 | 2011-05-25 | 张起凡 | Anti-virus feed additive for reducing cholesterol, production process thereof and application |
| CN101366455B (en) * | 2008-10-11 | 2011-02-02 | 唐山师范学院 | Processing method for preparing increasing milk agent for cow with seed cake enzymolysis peptide |
| CN102018098B (en) * | 2010-12-10 | 2013-06-12 | 江南大学 | Preparation method for fermented feed for corn-bean pulp type daily ration |
| CN106801021A (en) * | 2017-03-24 | 2017-06-06 | 广东瑞丰生态环境科技股份有限公司 | A kind of fermentation method for producing of novel buckle capsule laminating adhesive yeast |
| CN109362962B (en) * | 2018-12-28 | 2021-11-09 | 宁波天赋饲料科技有限公司 | Fermented feed capable of reducing heat stress of cows in middle and later lactation periods |
| CN111528346A (en) * | 2020-04-22 | 2020-08-14 | 华中农业大学 | Probiotic formula capable of improving feed intake of cattle and use method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1435113A (en) * | 2003-03-06 | 2003-08-13 | 天津科技大学 | Process for producing stalk protein feed by multi-culture united anaerobic fermentation |
| CN1559261A (en) * | 2004-02-23 | 2005-01-05 | 中国农业科学院畜牧研究所 | Two-effects microbiological additives of forage specially used for ruminants |
| CN1570082A (en) * | 2004-04-27 | 2005-01-26 | 包头市天赋食品有限公司 | Multiple strain microorganism ferment production method and its uses in cow fine fodder |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1435113A (en) * | 2003-03-06 | 2003-08-13 | 天津科技大学 | Process for producing stalk protein feed by multi-culture united anaerobic fermentation |
| CN1559261A (en) * | 2004-02-23 | 2005-01-05 | 中国农业科学院畜牧研究所 | Two-effects microbiological additives of forage specially used for ruminants |
| CN1570082A (en) * | 2004-04-27 | 2005-01-26 | 包头市天赋食品有限公司 | Multiple strain microorganism ferment production method and its uses in cow fine fodder |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283321A (en) * | 2010-06-18 | 2011-12-21 | 哈尔滨爱特杰牧业有限公司 | Cow rumen fermentation accelerator |
| CN102283321B (en) * | 2010-06-18 | 2012-08-22 | 哈尔滨爱特杰牧业有限公司 | Cow rumen fermentation accelerator |
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