CN106801021A - A kind of fermentation method for producing of novel buckle capsule laminating adhesive yeast - Google Patents
A kind of fermentation method for producing of novel buckle capsule laminating adhesive yeast Download PDFInfo
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- CN106801021A CN106801021A CN201710181208.1A CN201710181208A CN106801021A CN 106801021 A CN106801021 A CN 106801021A CN 201710181208 A CN201710181208 A CN 201710181208A CN 106801021 A CN106801021 A CN 106801021A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
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Abstract
The invention discloses a kind of fermentation process for detaining capsule laminating adhesive yeast, comprise the following steps：（1）It is prepared by liquid seed culture medium：Tested by medium optimization, draw the optimal liquid seed culture medium of culture denitrifying bacteria；（2）It is prepared by seed：Conventional bacterial screening and activation is first carried out, then culture obtains the liquid seeds in logarithmic phase in liquid seed culture medium；（3）The preparation of solid plate：Prepare the solid plate culture medium containing fluid nutrient medium composition；（4）Amplify fermentation：By step（2）In the liquid seeds that obtain be sprayed onto solid plate surface and close fermented and cultured in fermenting cellar；（5）Collect bacterium mud；（6）It is prepared by bacterium powder.The production method can produce button capsule laminating adhesive yeast in enormous quantities, and Saccharomycopsis fibuligera powder viable count is more, and production process operation difficulty is low, and raw material overall utilization rate is high, and low production cost, production efficiency are high.
Description
Technical field
The present invention relates to technical field of microbial fermentation, specifically, the present invention relates to a kind of novel buckle capsule laminating adhesive yeast
Fermentation method for producing.
Background technology
Industrial biotechnology overcomes the energy and environmental crisis caused by traditional chemical process mode, just increasingly causes
The attention of people.Starch is the primary raw material of industrial biotechnology, its abundance, cheap, is reproducible natural money
Source.Button capsule laminating adhesive yeast (Saccharomycopsis fibuligera), also known as Endomycopsis Fibnligera (Endomycopsis
Fibuligera), uncooked amylum α-amylase and carbohydrase can be expressed, it is considered to be produce the best ascus of amylolytic enzyme class
One of saccharomycete, biofermentation is carried out using capsule laminating adhesive yeast is detained, the various products required for starch being directly processed into people
Product and without liquefaction, saccharification, can energy saving, reduces cost, the processing for starch is significant, and button capsule laminating adhesive ferment
For female more traditional saccharomyces cerevisiae, with more rich secondary metabolism.However, the research on saccharomycopsis fibuligera bacterium is big
Many, in the optimization of the auxiliary of brewing industry, have no for the research in terms of its large-scale production, with button capsule laminating adhesive yeast
Deepization of physiological property research, the effect of button capsule laminating adhesive yeast is gradually by it is known that the demand for buckleing capsule laminating adhesive yeast is got over
Come bigger, thus need to seek it is a kind of can mass carry out detain capsule laminating adhesive yeast fermentation production method.
The content of the invention
Based on this, the invention reside in the defect for overcoming prior art, there is provided a kind of fermentation life of novel buckle capsule laminating adhesive yeast
Product method, the production method can produce button capsule laminating adhesive yeast in enormous quantities, and Saccharomycopsis fibuligera powder viable count is more, produces
Journey operation difficulty is low, and raw material overall utilization rate is high, and low production cost, production efficiency are high.
It is described another object of the present invention is to bacterium powder prepared by the fermentation method for producing for providing the button capsule laminating adhesive yeast
Bacterium powder is easy to storage, can directly be added when starch is processed and used.
Its technical scheme is as follows：
A kind of fermentation process for detaining capsule laminating adhesive yeast, comprises the following steps：
（1）It is prepared by liquid seed culture medium：Tested by medium optimization, draw the optimal liquid strain of culture denitrifying bacteria
Sub- culture medium, Medium Proportion is：8~12g of yeast extract, 18~22g of bacteriological peptone, 18~22g of malt flour, it is soluble
18~22g of starch, water 1L；
（2）It is prepared by seed：Conventional bacterial screening and activation is first carried out, the liquid seed culture medium after optimization is then inoculated in
In, 36~48h is cultivated in incubator, obtain the liquid seeds in logarithmic phase；
（3）The preparation of solid plate：In every 1L button capsule laminating adhesive yeast liquid seed culture mediums, 18~32g agar, sterilizing are added
After pour into flat board, after cooling i.e. can be made into the solid plate culture medium containing fluid nutrient medium composition；
（4）Amplify fermentation：By step（2）In the liquid seeds that obtain uniformly be sprayed on solid plate media surface, then place
In fermented and cultured is closed in 35 DEG C of ferment at constant temperature rooms, cultivate 24~36 hours；
（5）Collect bacterium mud：After fermentation ends, flat board is taken out into fermenting cellar, then with the thin slice after sterilizing, by plating medium
Bacterium mud scrape off, collect, obtain ultrahigh concentration bacterium mud；
（6）It is prepared by bacterium powder：Ultrahigh concentration bacterium mud is made bacterium powder.
The present invention first passes through medium optimization experiment and determines optimal liquid seed culture medium, and strain is through screening and activating
Culture obtains logarithmic phase liquid seeds in liquid seed culture medium afterwards, then makes logarithmic phase liquid seeds in solid plate culture medium
It is upper to amplify fermentation, it is collected into ultrahigh concentration bacterium mud and is made bacterium powder.Compared to liquid-state fermentation technology, fermentation process of the present invention
Viable bacteria inactivates caused by without to bacterium solution concentrate drying, reducing bacterium solution concentrate drying.Fermentation process of the present invention was produced
Journey operation difficulty is low, and raw material overall utilization rate is high, and the runoff water of generation is few, and Saccharomycopsis fibuligera powder viable count is more, production
Low cost, production efficiency are high.
Wherein in one embodiment, the Medium Proportion is：Yeast extract 10g, bacteriological peptone 20g, malt flour
20g, soluble starch 20g, water 1L.
Wherein in one embodiment, the strain density of the ultrahigh concentration bacterium mud is not less than 1.0 × 1013 cfu/g。
Wherein in one embodiment, the bacterium powder is prepared as：Ultrahigh concentration bacterium mud is mixed with sterilized water, obtains highly concentrated
Degree Saccharomycopsis fibuligera liquid, adds silica, is adsorbed by drying, and crosses 60 mesh sieves, after oversize is crushed again
60 mesh sieves are crossed, bacterium powder is obtained.
Wherein in one embodiment, the bacterium powder is prepared as：It is aided with after ultrahigh concentration bacterium mud is directly mixed with milk
Protective agent, spray-dried or freeze-drying is made bacterium powder.
Wherein in one embodiment, the protective agent is glycerine and/or sucrose.
The Saccharomycopsis fibuligera powder that the fermentation process of the button capsule laminating adhesive yeast is prepared.
Wherein in one embodiment, the bacterium powder viable count is not less than 4.0 × 1012 cfu/g。
Present invention also offers application of the Saccharomycopsis fibuligera powder in starch processing.By the button capsule laminating adhesive ferment
When female bacterium powder is added in starch, Saccharomycopsis fibuligera powder can express uncooked amylum α-amylase and carbohydrase, can make starch without
Need to liquefy, be saccharified by be directly processed into required product.
The beneficial effects of the present invention are：The invention discloses a kind of new button capsule laminating adhesive yeast fermentation process, first lead to
Cross medium optimization experiment and determine optimal liquid seed culture medium, strain is after screening and activation in liquid seed culture medium
Middle culture obtains logarithmic phase liquid seeds, then logarithmic phase liquid seeds is amplified fermentation in solid plate culture medium, is collected into
Ultrahigh concentration bacterium mud is simultaneously made bacterium powder, and the fermentation process production process operation difficulty is low, and raw material overall utilization rate is high, production effect
Rate is high, and the runoff water of generation is few, and bacterium powder viable count is more.
Specific embodiment
To make the objects, technical solutions and advantages of the present invention become more apparent, below in conjunction with specific embodiment, to this
Invention is described in further detail.It should be appreciated that specific embodiment described herein is only used to explain this hair
It is bright, do not limit protection scope of the present invention.
Embodiment 1
（1）It is prepared by liquid seed culture medium：Tested by medium optimization, draw the optimal liquid strain of culture denitrifying bacteria
Sub- culture medium, Medium Proportion is：Yeast extract 10g, bacteriological peptone 20g, malt flour 20g, soluble starch 20g, water
1L；
（2）It is prepared by seed：Conventional bacterial screening and activation is first carried out, the liquid seed culture medium after optimization is then inoculated in
In, 40h is cultivated in incubator, obtain the liquid seeds in logarithmic phase；
（3）The preparation of solid plate：In every 1L button capsule laminating adhesive yeast liquid seed culture mediums, 24g agar is added, fallen after sterilizing
Enter in flat board, the solid plate culture medium containing fluid nutrient medium composition is can be made into after cooling；
（4）Amplify fermentation：By step（2）In the liquid seeds that obtain uniformly be sprayed on solid plate media surface, then place
In fermented and cultured is closed in 35 DEG C of ferment at constant temperature rooms, cultivate 30 hours；
（5）Collect bacterium mud：After fermentation ends, flat board is taken out into fermenting cellar, then with the thin slice after sterilizing, by plating medium
Bacterium mud scrape off, collect, obtain ultrahigh concentration bacterium mud；
（6）It is prepared by bacterium powder：Ultrahigh concentration bacterium mud is mixed with sterilized water, high concentration Saccharomycopsis fibuligera liquid, addition two is obtained
Silica, is adsorbed by drying, and crosses 60 mesh sieves, crosses 60 mesh sieves after oversize is crushed again, is made 1500g bacterium powders.
Embodiment 2
（1）It is prepared by liquid seed culture medium：Tested by medium optimization, draw the optimal liquid strain of culture denitrifying bacteria
Sub- culture medium, Medium Proportion is：Yeast extract 8g, bacteriological peptone 22g, malt flour 22g, soluble starch 18g, water 1L；
（2）It is prepared by seed：Conventional bacterial screening and activation is first carried out, the liquid seed culture medium after optimization is then inoculated in
In, 36h is cultivated in incubator, obtain the liquid seeds in logarithmic phase；
（3）The preparation of solid plate：In every 1L button capsule laminating adhesive yeast liquid seed culture mediums, 18g agar is added, fallen after sterilizing
Enter in flat board, the solid plate culture medium containing fluid nutrient medium composition is can be made into after cooling；
（4）Amplify fermentation：By step（2）In the liquid seeds that obtain uniformly be sprayed on solid plate media surface, then place
In fermented and cultured is closed in 35 DEG C of ferment at constant temperature rooms, cultivate 36 hours；
（5）Collect bacterium mud：After fermentation ends, flat board is taken out into fermenting cellar, then with the thin slice after sterilizing, by plating medium
Bacterium mud scrape off, collect, obtain ultrahigh concentration bacterium mud；
（6）It is prepared by bacterium powder：Ultrahigh concentration bacterium mud is mixed with sterilized water, high concentration Saccharomycopsis fibuligera liquid, addition two is obtained
Silica, is adsorbed by drying, and crosses 60 mesh sieves, crosses 60 mesh sieves after oversize is crushed again, is made 1500g bacterium powders.
Embodiment 3
（1）It is prepared by liquid seed culture medium：Tested by medium optimization, draw the optimal liquid strain of culture denitrifying bacteria
Sub- culture medium, Medium Proportion is：Yeast extract 12g, bacteriological peptone 18g, malt flour 18g, soluble starch 22g, water
1L；
（2）It is prepared by seed：Conventional bacterial screening and activation is first carried out, the liquid seed culture medium after optimization is then inoculated in
In, 48h is cultivated in incubator, obtain the liquid seeds in logarithmic phase；
（3）The preparation of solid plate：In every 1L button capsule laminating adhesive yeast liquid seed culture mediums, 32g agar is added, fallen after sterilizing
Enter in flat board, the solid plate culture medium containing fluid nutrient medium composition is can be made into after cooling；
（4）Amplify fermentation：By step（2）In the liquid seeds that obtain uniformly be sprayed on solid plate media surface, then place
In fermented and cultured is closed in 35 DEG C of ferment at constant temperature rooms, cultivate 24 hours；
（5）Collect bacterium mud：After fermentation ends, flat board is taken out into fermenting cellar, then with the thin slice after sterilizing, by plating medium
Bacterium mud scrape off, collect, obtain ultrahigh concentration bacterium mud；
（6）It is prepared by bacterium powder：It is aided with protective agent glycerine and sucrose after ultrahigh concentration bacterium mud is directly mixed with milk, it is spray-dried
It is made 1500g bacterium powders.
Comparative example 1
With yeast extract 10g, bacteriological peptone 20g, malt flour 20g, soluble starch 20g, water 1L.Prepare fluid nutrient medium
10L, fermenting and producing is carried out by liquid fermentation process flow make-up capsule laminating adhesive yeast.Final products carry out concentrate drying, with dioxy
SiClx is inserts, is made 1500g bacterium powders.
Comparative example 2
With yeast extract 10g, bacteriological peptone 20g, malt flour 20g, soluble starch 20g, water 1L are seed culture medium, 500g
Wheat bran, 1000g cavings is solid matrix, and fermenting and producing is carried out with solid fermentation process flow, and final products drying is broken, is made
1500g bacterium powders.
Viable count measurement result is carried out to the bacterium powder of embodiment 1~3, comparative example 1, comparative example 2 using microbiological turbidity method
It is shown in Table 1.
Table 1
| Detection project | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 |
| Bacterium powder viable count/cfu/g |
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-described embodiment
In each technical characteristic it is all possible combination be all described, as long as however, the combination of these technical characteristics do not exist lance
Shield, is all considered to be the scope of this specification record.
Embodiment described above only expresses several embodiments of the invention, and its description is more specific and detailed, but simultaneously
Can not therefore be construed as limiting the scope of the patent.It should be pointed out that coming for one of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (7)
1. it is a kind of detain capsule laminating adhesive yeast fermentation process, it is characterised in that comprise the following steps：
（1）It is prepared by liquid seed culture medium：Tested by medium optimization, draw the optimal liquid strain of culture denitrifying bacteria
Sub- culture medium, Medium Proportion is：8~12g of yeast extract, 18~22g of bacteriological peptone, 18~22g of malt flour, solubility are formed sediment
18~22g of powder, water 1L；
（2）It is prepared by seed：Conventional bacterial screening and activation is first carried out, the liquid seed culture medium after optimization is then inoculated in
In, 36~48h is cultivated in incubator, obtain the liquid seeds in logarithmic phase；
（3）The preparation of solid plate：In every 1L button capsule laminating adhesive yeast liquid seed culture mediums, 18~32g agar, sterilizing are added
After pour into flat board, after cooling i.e. can be made into the solid plate culture medium containing fluid nutrient medium composition；
（4）Amplify fermentation：By step（2）In the liquid seeds that obtain uniformly be sprayed on solid plate media surface, then place
In fermented and cultured is closed in 35 DEG C of ferment at constant temperature rooms, cultivate 24~36 hours；
（5）Collect bacterium mud：After fermentation ends, flat board is taken out into fermenting cellar, then with the thin slice after sterilizing, by plating medium
Bacterium mud scrape off, collect, obtain ultrahigh concentration bacterium mud；
（6）It is prepared by bacterium powder：Ultrahigh concentration bacterium mud is made bacterium powder.
2. the fermentation process of capsule laminating adhesive yeast is detained according to claim 1, it is characterised in that the Medium Proportion is：Ferment
Female cream 10g, bacteriological peptone 20g, malt flour 20g, soluble starch 20g, water 1L.
3. the fermentation process of capsule laminating adhesive yeast is detained according to claim 1, it is characterised in that the bacterium powder is prepared as：Will be super
High concentration bacterium mud mixes with sterilized water, obtains high concentration Saccharomycopsis fibuligera liquid, adds silica, is adsorbed by drying,
60 mesh sieves are crossed, 60 mesh sieves are crossed after oversize is crushed again, bacterium powder is obtained.
4. the fermentation process of capsule laminating adhesive yeast is detained according to claim 1, it is characterised in that the bacterium powder is prepared as：Will be super
High concentration bacterium mud is aided with protective agent after directly mixing with milk, spray-dried or freeze-drying is made bacterium powder.
5. according to claim 4 detain capsule laminating adhesive yeast fermentation process, it is characterised in that the protective agent be glycerine and/
Or sucrose.
6. what the fermentation process of button capsule laminating adhesive yeast described in claim 1-5 any claims was prepared buckles capsule laminating adhesive yeast
Bacterium powder.
7. application of the Saccharomycopsis fibuligera powder described in claim 6 in starch processing.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710181208.1A CN106801021A (en) | 2017-03-24 | 2017-03-24 | A kind of fermentation method for producing of novel buckle capsule laminating adhesive yeast |
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| CN201710181208.1A CN106801021A (en) | 2017-03-24 | 2017-03-24 | A kind of fermentation method for producing of novel buckle capsule laminating adhesive yeast |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108660084A (en) * | 2018-05-23 | 2018-10-16 | 河南农业大学 | The preparation method of a large amount of button capsule laminating adhesive yeast |
| CN111876295A (en) * | 2020-08-10 | 2020-11-03 | 宜宾五粮液股份有限公司 | Mulberry fruit wine fermented by saccharomyces cerevisiae and preparation method thereof |
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| CN1685922A (en) * | 2005-04-29 | 2005-10-26 | 东北农业大学 | Composite life beneficial bacteria culture for improving milk cow production performance and its making method |
| CN105695373A (en) * | 2016-04-18 | 2016-06-22 | 广州市佰沃生物科技有限公司 | Bacillus subtilis(natto) fermentation method |
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2017
- 2017-03-24 CN CN201710181208.1A patent/CN106801021A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1685922A (en) * | 2005-04-29 | 2005-10-26 | 东北农业大学 | Composite life beneficial bacteria culture for improving milk cow production performance and its making method |
| CN105695373A (en) * | 2016-04-18 | 2016-06-22 | 广州市佰沃生物科技有限公司 | Bacillus subtilis(natto) fermentation method |
Non-Patent Citations (3)
| Title |
|---|
| 0. V. SARATHI REDDY AND S. C. BASAPPA: "Selection and Characterization of Endomycopsis fibdigera Strains for One-step Fermentation of Starch to Ethanol", 《STARCH》 * |
| DHANWANT K.SANDHU等: "Production of a-Amylase by Saccharomycopsis fibuligera(Syn. Endomycopsis fibuligera)", 《J.FERMENT.TEEHNOL.》 * |
| 应玲云等: "烧酒曲中扣囊复膜酵母的分离及鉴定", 《食品与发酵工业》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108660084A (en) * | 2018-05-23 | 2018-10-16 | 河南农业大学 | The preparation method of a large amount of button capsule laminating adhesive yeast |
| CN108660084B (en) * | 2018-05-23 | 2021-11-12 | 河南农业大学 | Preparation method of large amount of Saccharomycopsis fibuligera |
| CN111876295A (en) * | 2020-08-10 | 2020-11-03 | 宜宾五粮液股份有限公司 | Mulberry fruit wine fermented by saccharomyces cerevisiae and preparation method thereof |
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Application publication date: 20170606 |