CN105558303A - Yeast culture added with Chinese medicinal herb formulation and preparation method of yeast culture - Google Patents
Yeast culture added with Chinese medicinal herb formulation and preparation method of yeast culture Download PDFInfo
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Abstract
The invention discloses a preparation method of a yeast culture added with Chinese medicinal herb extract. On the basis of optimizing a conventional process for producing feed through yeast fermentation and combining liquid-solid phases, the Chinese medicinal herb extract is added, so that absorption and utilization of nutritional substances by livestock and poultry are improved and disease resistance of livestock and poultry is improved. The preparation method particularly includes steps of seed activated culturing, strain amplified culturing, liquid deep fermentation, solid aerobic fermentation, solid anaerobic fermentation, low-temperature environment-friendly drying and smashing and packaging.
Description
Technical field
The invention belongs to fermented feed technical field, the present invention relates to a kind of yeast culture adding Chinese herbal medicine formula and preparation method thereof.
Background technology
Probiotics, antibiotic best substitute in 21 century feed addictive, in culture fishery, and be widely applied in livestock and poultry animal aquaculture, that one has no side effect, noresidue, the pure natural fodder additive product had no drug resistance, and yeast culture is wherein applied a kind of rather widely especially.
Yeast culture (YeastCulture), as a kind of probiotics, also or be called symphysis unit, by yeast living cells, yeast metabolism product and culture medium composition thereof, form through specific zymotechnique anaerobic fermentation, abundant nutriment can be provided, as B family vitamin, amino acid, the short factor ~ the enzyme of digestion, organic acid (lactic acid, malic acid, formic acid, butanedioic acid), mineral matter and some ester classes, alcohols material and UGF etc., simultaneously, because of β ~ glucan, the materials such as mannosan have certain immunogenicity, with health role again, as balance intestinal microbiota, suppress pathogenic microorganism breeding thus improve animal immunizing power, Chinese scholars proves through large quantifier elimination, yeast culture is for breeding performonce fo animals, function of intestinal canal regulation and control etc. all have very large effect, the body weight of beef cattle can be increased as added YC in daily ration, feed efficiency, also lumen fermentation can be changed, increase cud lactic acid and utilize bacterium and cellulose-decomposing bacteria, increase digestibility, increase rumen bacteria concentration etc., but, probiotics is limited for the preventive and therapeutic effect of Animal diseases, and result of use cannot to embody in a short time be the large drawback of one.
Add Chinese herbal medicine extract, for animal disease resistant poison, improve immunity, digestibility, tonifying Qi and lifting yang, strengthening exterior and reducing sweat, promoting pus discharge and tissue regeneration etc. have remarkable result.In view of this, the object of the invention is to propose a kind of production method of adding the yeast culture of medicinal herb components, improve animal immunizing power, increase raiser's economic benefit.
Summary of the invention
In view of this, the invention provides a kind of yeast culture adding Chinese herbal medicine formula and preparation method thereof, by the optimization of zymotechnique, shorten fermentation period, reduce production cost, simple and easy Controlling Technology, carrying out large-scale production, by adding Chinese herbal and crude drugs preparations, improving the medicinal function of product.Meanwhile, obtained yeast culture product good palatability, can not only provide abundant nutriment, and play the effect of health care.
The present invention realizes by the following technical solutions:
A kind of preparation method adding the yeast culture of Chinese herbal medicine formula of the present invention, comprises following step:
1) with YEPD liquid basal medium, rejuvenation activation is carried out to yeast strain, cultivate, be then inoculated in shake-flask seed culture medium, then amplify cultivation by seeding tank step by step to fermentation tank, obtain yeast liquid fermentation culture;
2) mixed by a certain percentage with solid medium by Chinese recipe, described Chinese recipe is astragalus polyose medicinal extract and Salvia root P.E mixture;
3) mixed with solid medium by above-mentioned saccharomycete nutrient solution, be aerobic fermentation 18 ~ 24h under the condition of 25 ~ 35 DEG C in temperature, anaerobic fermentation is 24 ~ 48h about, is solid fermentation product.
Further, described yeast strain is seed selection height vigor bacterial classification voluntarily.
Further, through step 1) aerobic fermentation after, saccharomycete viable count reaches 8 ~ 1,000,000,000 CFU/mL.
Further, in described step 3) in, described aerobic fermentation condition is: relative air humidity 60% ~ 70%, and the mixed moisture of solid medium of described saccharomycetes to make fermentation bacterium liquid and inoculation controls 50% ~ 60%, inoculum concentration is that 30% ~ 60%, pH controls 4.0 ~ 5.5.
Further, in described step 1) in, by described saccharomycete seed culture fluid with 1% ~ 3% inoculum concentration be seeded in dextrose broth expand numerous, aerobic fermentation 16 ~ 20 hours under 28 ~ 30 DEG C of conditions, obtained described yeast liquid fermentation culture.
Further, consisting of of described YEPD liquid basal medium: glucose: 15 ~ 30g/L, peptone: 15 ~ 25g/L, yeast extract 5 ~ 10g/L; One-level shake-flask seed culture medium consists of: glucose: 20 ~ 40g/L, sucrose: 20 ~ 40g/L, peptone: 5 ~ 10g/L, yeast extract: 10 ~ 20g/L, KH2PO4:0.5 ~ 1g/L, MgSO4:0.2 ~ 0.5g/L, initial pH5.5 ~ 6.0,115 DEG C of high pressure 20min, control this nutrient solution rotating speed at 160 ~ 180r/min after inoculation.
Further, in described step 1), to utilize submerged-culture method to carry out expansion numerous for fermentation tank, namely by the cultural method of pot bottom air agitation, medium component proportioning is: glucose: 15 ~ 30g/L, peptone: 5 ~ 15g/L, yeast extract: 10 ~ 20g/L, KH2PO4:0.5 ~ 1g/L, MgSO4:0.2 ~ 0.5g/L, wherein, when starting feed supplement after liquid fermentation 10h, supplemented medium forms: glucose: 15 ~ 30g/L, peptone: 2 ~ 5g/L, urea: 2 ~ 5g/L, totally 4 ~ 6 times, every 2 ~ 3 hours once.
Further, in described step 2) in, pulverize raw materials used in advance, with aperture 1.2mm screen filtration.
Further, the solid medium in described step 3) is made up of according to mass percent following material: corn flour: 50 ~ 60%, wheat bran: 30 ~ 40%, dregs of beans: 10 ~ 12%, Chinese herbal medicine extract: 0.05 ~ 0.5%; Or corn flour: 30 ~ 35%, maize peel: 25 ~ 40%, dregs of beans: 10 ~ 12%, wheat bran: 10 ~ 12%, rice bran: 10 ~ 12%, Chinese herbal medicine extract: 0.05 ~ 0.5%.
A kind of yeast culture, described yeast culture prepares according to the preparation method of the yeast culture in claim 1 ~ 9 described in any one.
A kind of yeast culture adding Chinese herbal medicine formula of the present invention and preparation method thereof has following beneficial effect:
In the preparation process of yeast culture provided by the invention, by saccharomycete liquid fermentation technology optimization, not only effectively shorten the production cycle, increase feed palatability, meanwhile, the drug action of Chinese herbal medicine extract also reaches and gives full play to, and makes a kind of yeast culture with unique function.
Major advantage of the present invention has: production technology cycle time, reduces production cost, and provides rich in protein, vitamin, the nutrition such as glycan and immune substance.
This product, except having the distinctive Nutrition and health function of yeast culture, also has unique pharmacological action, makes an addition in the feed of livestock and poultry, improves its adaptive capacity to environmental stress, strengthens its immunity and premunition, increase economic efficiency.
Detailed description of the invention
The present invention is described in detail below.
A kind of preparation method adding the yeast culture of Chinese herbal medicine formula of the present invention, comprises the following steps:
1), by bacterial classification carry out rejuvenation respectively, be seeded to Shaking culture, then carry out fermentation tank expand step by step aerobic fermentation cultivate, produce yeast liquid fermentation culture.
2), in proportion Chinese herbal medicine extract and solid fermentation culture medium are mixed, for subsequent use.
3), in proportion the above-mentioned yeast liquid fermentation culture that makes and Chinese herbal medicine extract mixed solid state cuture base are mixed, temperature controls 25 ~ 35 DEG C, and aerobic fermentation 18 ~ 24h, obtains solid fermentation head product;
4), by above-mentioned solid fermentation product carry out anaerobic fermentation 24 ~ 48h, be yeast culture;
5), by the yeast culture obtained carry out low-temperature environment-friendly dry, pulverize, packaging.
As the further improvement of technique scheme, yeast starter tank nutrient solution is inoculated into 7T fermentation tank with the inoculum concentration of 6 ~ 10%, after inoculation, fermentation tank slowly rotates 2 hours, allows barms fully hatch in isoperibol, and zymocyte liquid pH controls 4.0 ~ 5.5, during fermentation 20 ~ 24h, nutrient solution viable count reaches 8 ~ 1,000,000,000/mL.
As the further improvement of technique scheme, above-mentioned steps 3) in, its aerobic fermentation condition is: relative air humidity 60 ~ 70%, the moisture that described liquid fermentation and culture liquid mixes with the solid fermentation culture medium adding Chinese herbal medicine extract controls to be 50 ~ 60%, with upender 2 ~ 4 hours stirrings once, guarantee aerobic fermentation.
As the further improvement of technique scheme, in solid aerobic fermentation, viable count can reach 8 ~ 1,000,000,000 CFU/g.
As the further improvement of technique scheme, shake-flask seed liquid culture medium forms:
Glucose: 20 ~ 40g/L, sucrose: 20 ~ 40g/L, peptone: 5 ~ 10g/L, yeast extract: 10 ~ 20g/L, KH2PO4:0.5 ~ 1g/L, MgSO4:0.2 ~ 0.5g/L. initial pH5.5,115 DEG C of high pressure 20min.
As the further improvement of technique scheme, 7T ferment tank culture medium forms:
Fermentation tank culture medium composition proportion is: glucose: 15 ~ 30g/L, peptone: 5 ~ 15g/L, yeast extract: 10 ~ 20g/L, KH2PO4:0.5 ~ 1g/L, MgSO4:0.2 ~ 0.5g/L, wherein, when starting feed supplement after liquid fermentation 10h, supplemented medium forms: glucose: 15 ~ 30g/L, peptone: 2 ~ 5g/L, urea: 2 ~ 5g/L, altogether feed supplement 4 ~ 6 times, every 2 ~ 3 hours once.
As the further improvement of technique scheme, described solid fermentation culture medium composition:
Corn flour: 50 ~ 60%, wheat bran: 30 ~ 40%, dregs of beans: 10 ~ 12%, Chinese herbal medicine extract: 0.05 ~ 1%;
Or, corn flour: 30 ~ 35%, maize peel: 25 ~ 40%, dregs of beans: 10 ~ 12%, wheat bran: 10 ~ 12%, rice bran: 10 ~ 12%, Chinese herbal medicine extract: 0.05 ~ 0.5%.
Find out from above, in the preparation process of yeast culture provided by the invention, by saccharomycete liquid fermentation technology optimization, not only effectively shorten the production cycle, increase feed palatability, meanwhile, the drug action of Chinese herbal medicine extract also reaches and gives full play to, and makes a kind of yeast culture with unique function.
Embodiment 1:
In embodiment of the present invention, the yeast culture of preparation comprises the following steps:
(1) with basic YEPD fluid nutrient medium, rejuvenation is carried out to production bacterium: picking one ring inclined-plane bacterial strain, be inoculated in seed liquor culture medium, 150ml triangular flask culture medium loading amount is 50ml, 28 ~ 30 DEG C, 180 ~ 200r/min Shaking culture, 16 ~ 18h, this is primary seed solution, by this seed liquor with in the inoculum concentration access secondary seed liquid culture medium of 2%, 1000ml triangular flask liquid amount 360ml, 28 DEG C ~ 30 DEG C, 180 ~ 200r/min Shaking culture, 16 ~ 18h; Wherein, basic YEPD Liquid Culture based component is: glucose 10 ~ 20g/L, peptone 10 ~ 20g/L, yeast extract 5 ~ 10g/L, initial pH5.5; Secondary seed nutrient solution composition is: glucose: 20 ~ 40g/L, sucrose: 20 ~ 40g/L, peptone: 5 ~ 10g/L, yeast extract: the initial pH5.5 of 10 ~ 20g/L, KH2PO4:0.5 ~ 1g/L, MgSO4:0.2 ~ 0.5g/L.; Above-mentioned two kinds of culture mediums all high-temperature sterilization 25min under 115 DEG C of conditions.
Then, this seed culture fluid is carried out expansion with the seed fermentation tank of the inoculum concentration access 500L of 1 ~ 3% numerous, control temperature is 28 ~ 30 DEG C, aerobic fermentation 16 ~ 20 hours, wherein, just should slow rotary rotor during access, adjusting rotary speed is 40r/min, after 2 hours, rotating speed is adjusted to 60 ~ 180r/min, obtained culture propagation seed liquor, wherein, seeding tank fluid nutrient medium is: glucose: 15 ~ 30g/L, peptone: 5 ~ 10g/L, yeast extract: 10 ~ 20g/L, KH2PO4:0.5 ~ 1g/L, the initial pH5.5 of MgSO4:0.2 ~ 0.5g/L., 115 DEG C of high pressure 20min.
Below, by this seed culture fluid with 6 ~ 10% inoculum concentration access 7T fermentation tank, control temperature is 28 ~ 32 DEG C, aerobic fermentation 20 ~ 24 hours, wherein 7T ferment tank culture medium consists of: glucose: 15 ~ 30g/L, peptone: 5 ~ 15g/L, yeast extract: 10 ~ 20g/L, KH2PO4:0.5 ~ 1g/L, MgSO4:0.2 ~ 0.5g/L; Wherein, when starting feed supplement after liquid fermentation 10h, supplemented medium forms: glucose: 15 ~ 30g/L, peptone: 2 ~ 5g/L, urea: 2 ~ 5g/L, totally 4 ~ 6 times, every 2 ~ 3 hours quantitative material supplementings once.
(2) pulverized in advance by solid culture based raw material, with aperture 1.2mm screen filtration, mix in proportion with Chinese herbal medicine extract, wherein, solid medium is corn flour: 50 ~ 60%, wheat bran: 30 ~ 40%, dregs of beans: 10 ~ 12%, Chinese herbal medicine extract: 0.05 ~ 0.1%;
(3) the Yeast Cultivation liquid (bacteria containing amount is 8 ~ 1,000,000,000 CFU/mL) step 1) obtained and step 2) solid medium batch mixer mix, its moisture controls 50 ~ 60%, 25 ~ 35 DEG C of aerobic fermentations 18 ~ 24 hours, obtains solid fermentation head product.Wherein, in this process, controlling relative air humidity is that 60% ~ 70%, pH controls 4.0 ~ 5.0, can the growth of effective relation control miscellaneous bacteria.
(4) above-mentioned solid fermentation product is carried out anaerobic fermentation, ferment after 24 ~ 48 hours, carried out low temperature drying, pulverize, be yeast culture after packaging, this product has the special alcohol fragrance of fermentation.
Embodiment 2 is the further embodiments on embodiment 1 basis, described with corn flour, wheat bran, and dregs of beans is the Chinese herbal medicine extract addition of solid base fermentation is 0.1 ~ 0.5%.
Embodiment 3 is the further embodiments on embodiment 1 basis, described with corn flour, wheat bran, dregs of beans, maize peel, and rice bran is the Chinese herbal medicine extract addition of solid base fermentation is 0.05 ~ 0.1%.
Embodiment 4 is the further embodiments on embodiment 1 basis, described with corn flour, wheat bran, dregs of beans, maize peel, and rice bran is the Chinese herbal medicine extract addition of solid base fermentation is 0.1 ~ 5%.
The yeast culture Testing index contrast that embodiment 1 ~ 4 obtains is as shown in table 1:
| Glycan | Crude protein | Coarse ash | Moisture | |
| Embodiment 1 | 2.22% | 18.88% | 4.00% | 9.08% |
| Embodiment 2 | 2.61% | 19.86% | 4.04% | 8.90% |
| Embodiment 3 | 2.87% | 20.29% | 4.80% | 7.35% |
| Embodiment 4 | 2.97% | 20.22% | 4.85% | 8.22% |
Table 1 yeast culture Testing index contrast table
Each embodiment in this description all adopts the mode of going forward one by one to describe, and what each embodiment stressed is the difference with other embodiments, between each embodiment identical similar part mutually see.
Although described the preferred embodiment of the embodiment of the present invention, those skilled in the art once obtain the basic creative concept of cicada, then can make other change and amendment to these embodiments.So claims are intended to be interpreted as comprising preferred embodiment and falling into all changes and the amendment of embodiment of the present invention scope.
What finally illustrate is, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although with reference to preferred embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not departing from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (10)
1. add a preparation method for the yeast culture of Chinese herbal medicine formula, it is characterized in that, comprise following step:
1) with YEPD liquid basal medium, rejuvenation activation is carried out to yeast strain, cultivate, be then inoculated in shake-flask seed culture medium, then amplify cultivation by seeding tank step by step to fermentation tank, obtain yeast liquid fermentation culture;
2) mixed by a certain percentage with solid medium by Chinese recipe, described Chinese recipe is astragalus polyose medicinal extract and Salvia root P.E mixture;
3) mixed with solid medium by above-mentioned saccharomycete nutrient solution, be aerobic fermentation 18 ~ 24h under the condition of 25 ~ 35 DEG C in temperature, anaerobic fermentation is 24 ~ 48h about, is solid fermentation product.
2. a kind of preparation method adding the yeast culture of Chinese herbal medicine formula according to claim 1, is characterized in that, described yeast strain is seed selection height vigor bacterial classification voluntarily.
3. a kind of preparation method adding the yeast culture of Chinese herbal medicine formula according to claim 1, is characterized in that, through step 1) aerobic fermentation after, saccharomycete viable count reaches 8 ~ 1,000,000,000 CFU/mL.
4. a kind of preparation method adding the yeast culture of Chinese herbal medicine formula according to claim 1, it is characterized in that, in described step 3) in, described aerobic fermentation condition is: relative air humidity 60% ~ 70%, the mixed moisture of solid medium of described saccharomycetes to make fermentation bacterium liquid and inoculation controls 50% ~ 60%, inoculum concentration is that 30% ~ 60%, pH controls 4.0 ~ 5.5.
5. a kind of preparation method adding the yeast culture of Chinese herbal medicine formula according to claim 1, it is characterized in that, in described step 1) in, by described saccharomycete seed culture fluid with 1% ~ 3% inoculum concentration be seeded in dextrose broth expand numerous, aerobic fermentation 16 ~ 20 hours under 28 ~ 30 DEG C of conditions, obtained described yeast liquid fermentation culture.
6. a kind of preparation method adding the yeast culture of Chinese herbal medicine formula according to claim 1, is characterized in that, consisting of of described YEPD liquid basal medium: glucose: 15 ~ 30g/L, peptone: 15 ~ 25g/L, yeast extract 5 ~ 10g/L; One-level shake-flask seed culture medium consists of: glucose: 20 ~ 40g/L, sucrose: 20 ~ 40g/L, peptone: 5 ~ 10g/L, yeast extract: 10 ~ 20g/L, KH2PO4:0.5 ~ 1g/L, MgSO4:0.2 ~ 0.5g/L, initial pH5.5 ~ 6.0,115 DEG C of high pressure 20min, control this nutrient solution rotating speed at 160 ~ 180r/min after inoculation.
7. a kind of preparation method adding the yeast culture of Chinese herbal medicine formula according to claim 1, it is characterized in that, in described step 1), to utilize submerged-culture method to carry out expansion numerous for fermentation tank, namely by the cultural method of pot bottom air agitation, medium component proportioning is: glucose: 15 ~ 30g/L, peptone: 5 ~ 15g/L, yeast extract: 10 ~ 20g/L, KH2PO4:0.5 ~ 1g/L, MgSO4:0.2 ~ 0.5g/L, wherein, when starting feed supplement after liquid fermentation 10h, supplemented medium forms: glucose: 15 ~ 30g/L, peptone: 2 ~ 5g/L, urea: 2 ~ 5g/L, totally 4 ~ 6 times, every 2 ~ 3 hours once.
8. a kind of preparation method adding the yeast culture of Chinese herbal medicine formula according to claim 1, is characterized in that, in described step 2) in, pulverize raw materials used in advance, with aperture 1.2mm screen filtration.
9. a kind of preparation method adding the yeast culture of Chinese herbal medicine formula according to claim 1, it is characterized in that, solid medium in described step 3) is made up of according to mass percent following material: corn flour: 50 ~ 60%, wheat bran: 30 ~ 40%, dregs of beans: 10 ~ 12%, Chinese herbal medicine extract: 0.05 ~ 0.5%; Or corn flour: 30 ~ 35%, maize peel: 25 ~ 40%, dregs of beans: 10 ~ 12%, wheat bran: 10 ~ 12%, rice bran: 10 ~ 12%, Chinese herbal medicine extract: 0.05 ~ 0.5%.
10. a yeast culture, is characterized in that, described yeast culture prepares according to the preparation method of the yeast culture in claim 1 ~ 9 described in any one.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105519791A (en) * | 2016-01-27 | 2016-04-27 | 郭礼强 | Preparation method of traditional Chinese medicine and pollen feed for bumblebees |
| CN106929437A (en) * | 2017-04-25 | 2017-07-07 | 西安鑫汉宝生物科技有限公司 | A kind of preparation method of the yeast culture of wine yeast fermentation |
| CN106967769A (en) * | 2017-03-23 | 2017-07-21 | 河南省农业科学院园艺研究所 | Improve fermentation medium and cultural method that pale purple purple spore bacterium produces leucinostatin |
| CN107410701A (en) * | 2017-04-25 | 2017-12-01 | 西安鑫汉宝生物科技有限公司 | A kind of method of sweet sorghum stalk fermented feed and application thereof |
| CN107788249A (en) * | 2017-12-08 | 2018-03-13 | 雷山万城经济开发商贸有限公司 | A kind of agent of feed for pregnancy sow and preparation method thereof |
| CN107950783A (en) * | 2017-12-12 | 2018-04-24 | 湖南德人牧业科技有限公司 | A kind of fine fodder for preventing mastitis for milk cows and preparation method thereof, equipment |
Citations (1)
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| CN101558823A (en) * | 2008-04-15 | 2009-10-21 | 北京大北农科技集团股份有限公司 | Fermented liquid feed for Pig and poultry as well as preparation method and application thereof |
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- 2015-12-23 CN CN201510972685.0A patent/CN105558303A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101558823A (en) * | 2008-04-15 | 2009-10-21 | 北京大北农科技集团股份有限公司 | Fermented liquid feed for Pig and poultry as well as preparation method and application thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105519791A (en) * | 2016-01-27 | 2016-04-27 | 郭礼强 | Preparation method of traditional Chinese medicine and pollen feed for bumblebees |
| CN106967769A (en) * | 2017-03-23 | 2017-07-21 | 河南省农业科学院园艺研究所 | Improve fermentation medium and cultural method that pale purple purple spore bacterium produces leucinostatin |
| CN106967769B (en) * | 2017-03-23 | 2021-04-23 | 河南省农业科学院园艺研究所 | Fermentation medium and culture method for improving leucotrichin production of lilac purpurea |
| CN106929437A (en) * | 2017-04-25 | 2017-07-07 | 西安鑫汉宝生物科技有限公司 | A kind of preparation method of the yeast culture of wine yeast fermentation |
| CN107410701A (en) * | 2017-04-25 | 2017-12-01 | 西安鑫汉宝生物科技有限公司 | A kind of method of sweet sorghum stalk fermented feed and application thereof |
| CN107788249A (en) * | 2017-12-08 | 2018-03-13 | 雷山万城经济开发商贸有限公司 | A kind of agent of feed for pregnancy sow and preparation method thereof |
| CN107950783A (en) * | 2017-12-12 | 2018-04-24 | 湖南德人牧业科技有限公司 | A kind of fine fodder for preventing mastitis for milk cows and preparation method thereof, equipment |
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