CN1559261A - Two-effects microbiological additives of forage specially used for ruminants - Google Patents
Two-effects microbiological additives of forage specially used for ruminants Download PDFInfo
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- Fodder In General (AREA)
Abstract
A dual-effect microbial feed additive for ruminant is prepared through pulverizing corn, steaming, liquefying, saccharified, inoculating lactobacillus acidophilus and saccharomyce cerevisiae, fermenting to obtain living bacterium preparation, culturing bacillus subtilis, aspergillus oryzae and aspergillus niger in the culture medium prepared from corn, bran and soybean dregs, fermenting to obtain composite enzyme preparation, and proportionally mixing said living bacterium preparation with said composite enzyme preparation. It can promote the growth and health of ruminant and increase the utilization rate of feed.
Description
One technical field
The present invention relates to special-purpose economic benefits and social benefits additive for microbe feedstuff of a kind of ruminant and preparation method thereof, belong to the additive for microbe feedstuff technical field.
Two background technologies
Feed safety is the significant problem of current whole world extensive concern.Add medicament residue and the generation of the pathogen resistance to the action of a drug that antibiotic and certain drug cause in feed, this has become a global difficult problem, and more and more countries takes the legislation means to forbid abuse of antibiotics and medicine.The additive for microbe feedstuff of development of new safety, substitute or alternative antibiotic of part and the application of medicine in livestock and poultry breeding industry, for alleviating feed and food-safety problem such as the residual and pathogen drug resistance of livestock products Chinese traditional medicine, ensure human health to have positive meaning.
Ruminant is because its unique physilogical characteristics, can utilize low value green and rough feeds and agricultural byproducts to be converted into the livestock products such as meat milk of high nutritive value, the feed efficiency height, culture benefit is obvious, and the ruminant aquaculture is an industry most effective in the modern agriculture industrial structure.Ruminant aquacultures such as development milk cow, beef cattle and mutton sheep etc. are alleviated the situation of China's grain relative shortage for making full use of feed resource, transform agricultural production, developing rural economy, aspect such as increase farmers' income be of great immediate significance.The additive for microbe feedstuff of exploitation ruminant special use, can improve the ruminant tumor gastric fermenting property effectively, improve the utilization rate and the conversion ratio of feed (especially roughage), improve the health status of animal body, reduce disease incident, avoid safety issues such as medicament residue and drug resistance, for the mankind provide more high-quality livestock products.
At present, domestic and international research and development about the ruminant additive for microbe feedstuff, mainly concentrate on two classes: a class is yeast culture and yeast active bacteria formulation, with the saccharomyces cerevisiae is that starting strain is prepared from by fermentation, contain active ingredient such as the active factors of growth of microorganism and living cells in the regulation and control animal alimentary canal, have regulation and control to improve the microbiotic effect of animal alimentary canal; One class is an enzyme preparation, microorganism with high enzymatic productivity is a starting strain, process solid or liquid fermentation and certain extraction process are prepared from, be generally and contain protease, amylase, lipase, cellulase, hemicellulase isoreactivity composition, can improve the utilization ratio of animal various components in the feed.This two series products all belongs to the additive product of single effect, does not directly possess microecological regulation and control and feed nutrient utilization and promotes two kinds of effects.
The purpose of this invention is to provide special-purpose economic benefits and social benefits additive for microbe feedstuff of a kind of ruminant and preparation method thereof.After adopting the present invention, can improve the feed intake of milk cow and, improve the calf daily gain and be up to 46.75%, reduce feedstuff-meat ratio and be up to 31.7%, improve milk production of cow and reach 6.30%, significantly increase economic benefit the utilization ratio of roughage.
Three summary of the invention
The special-purpose economic benefits and social benefits microbe additive of a kind of ruminant, it is that the employing corn is a raw material, through steps such as pulverizing, boiling, liquefaction, saccharification, make corn liquefaction enzymolysis liquid, as the fermentation raw material of lactobacillus acidophilus (Lactobacillus acidophilus) and saccharomyces cerevisiae (Saccharomyce cerevisiae), fermentation is prepared into the active bacteria formulation of lactobacillus acidophilus and saccharomyces cerevisiae culture respectively; Adopt the enzymatic production culture medium of raw materials such as corn, wheat bran and dregs of beans as bacillus subtilis (Bacillus subtilis), aspergillus oryzae (Aspergillusoryzae) and aspergillus niger (Aspergillus niger), bacillus subtilis is used liquid fermentation, aspergillus oryzae and aspergillus niger solid mixed culture fermentation, and compatibility is made complex enzyme formulation; With active bacteria formulation and complex enzyme formulation in the composite dual-purpose additive product of making of 1: 4 ratio.The every gram of this invention product contains beneficial microbe viable count＞10
9Cfu, AMS is lived and to be every gram product per minute hydrolyzed starch amount＞4000 milligrams, protease activity is that every gram product per minute caseinhydrolysate generates amount of amino acid＞500 milligram, cellulase activity is every gram product per minute degraded cellulose amount＞200 milligrams, and lipase activity is every gram product per minute hydrolyzing triglyceride amount＞150 milligrams.
Preparation method of the present invention is
(1) active bacteria formulation preparation
1 bacterial classification
Lactobacillus acidophilus (Lactobacillus acidophilus), saccharomyces cerevisiae (Saccharomyce cerevisiae).
2 raw materials
After adopting fresh yellow maize to pulverize to handle, be that 1: 3.0～4.0 ratio adds 50 ℃ of warm water spices, be heated to 80 ℃ after mixing thoroughly and precooked 30～40 minutes, at 132～135 ℃, 2.8～3.0kg/cm in material-water ratio
2Boiling is 90 minutes under the condition, is cooled to about 65 ℃, adds high temperature Ye Huamei and carbohydrase and handles 3～4 hours, obtain the DE value and be more than 60%, pol is at the corn enzymatic saccharification liquid of 12～14 ° of Bx, regulating the pH value is about 4.5, is heated to about 90 ℃ to keep 30 minutes again, stand-by.
3 zymotechniques
(1) lactobacillus acidophilus (Lactobacillus acidophilus)
A. culture medium
Seed culture medium (g/1000ml): soy peptone 10g, beef extract 10g, yeast extract 5g, K
2HPO
42g, dibasic ammonium citrate 2g, sodium acetate 5g, glucose 20g, Tween 80 1mL, MgSO
47H
2O 0.58g, MnSO
44H
2O 0.25g, pH6.2 sterilized 20 minutes for 115 ℃.
Fermentation medium: corn enzymatic saccharification liquid adds 3% defatted soy flour, sterilizes 30 minutes for 121 ℃.
B. condition of culture
First order seed is cultivated: be shake flask fermentation, get the bottled seed culture medium 50ml of 250ml triangle, get slant activation bacterial classification 1 articulating and go in the culture medium, 30 ℃, the 220rpm rotary shaker was cultivated 36 hours.
Secondary seed is cultivated: the 10L automated seed canned seed culture medium 6L that ferments, by 5% inoculum concentration, 30 ℃, 300rpm cultivated 28 hours.
The full-automatic stirred fermentor dress of fermentation tank culture: 100L fermentation medium 60L adds inoculation by seeding tank stream, 10%, 30 ℃ of inoculum concentration, and 300rpm cultivated 28～32 hours.
(2) saccharomyces cerevisiae (Saccharomyce cerevisiae)
A. culture medium
Seed culture medium: get fresh moyashi and add water in 1: 10 ratio, boiled 30 minutes, use filtered through gauze, filtrate adds water and supplies initial volume, adds glucose 5%, and natural pH value was sterilized 30 minutes for 115 ℃.
Fermentation medium: corn enzymatic saccharification liquid.
B. condition of culture
First order seed is cultivated: be shake flask fermentation, get the bottled seed culture medium 50ml of 250ml triangle, get slant activation bacterial classification 1 articulating and go in the culture medium, 28 ℃, 260rpm cultivated 16 hours.
Secondary seed is cultivated: the 10L automated seed canned fermentation medium 6L that ferments, by 5% inoculum concentration, 28 ℃, 300rpm cultivated 18 hours.
The full-automatic stirred fermentor dress of fermentation tank culture: 100L fermentation medium 60L adds inoculation by seeding tank stream, 10%, 28 ℃ of inoculum concentration, and 200rpm cultivated 18～24 hours.
4 dry processing
Add in the zymotic fluid 0.5% whey powder as protective agent after, pass through spray-drying respectively, make lactobacillus acidophilus cultures (viable count＞1.5 * 10
9Cfu/g) and yeast culture (viable count＞1.0 * 10
10Cfu/g), the two is re-dubbed viable count in 40: 60 ratio and surpasses 6.6 * 10
9The active bacteria formulation of cfu/g.
(2) enzyme preparation
1 bacterial classification
Bacillus subtilis (Bacillus subtilis), aspergillus oryzae (Aspergillus oryzae) and aspergillus niger (Aspergillus niger).
2 zymotechniques
(1) bacillus subtilis (Bacillus subtilis)
A. culture medium
Seed culture medium: soya-bean cake powder 3%, corn flour 2%, wheat bran 3%, Na
2HPO
40.4%, KH
2PO
40.03%, natural pH value was sterilized 20 minutes for 121 ℃.
Fermentation medium: soya-bean cake powder 3%, corn flour 5%, wheat bran 4%, Na
2HPO
40.4%, KH
2PO
40.03%, natural pH value was sterilized 20 minutes for 121 ℃.
Feeding medium during fermentation liquid: get silkworm chrysalis: lime: water mixes, 1.0 * 10 in 100: 6: 600 ratio
5Heating hydrolysis is 1 hour under the Pa steam pressure, makes the silkworm chrysalis hydrolyzate, as feeding medium during fermentation liquid.
B. condition of culture
Seed culture: the 10L automated seed canned fermentation medium 6L that ferments, get the cell suspension (about 1.0 * 10 that slant strains is made
11Cfu/ml) insert seeding tank by 1% inoculum concentration, 30 ℃, 1: 1.2 volume ratio of ventilation, 350rpm cultivated 8 hours.
The full-automatic stirred fermentor dress of fermented and cultured: 100L fermentation medium 60L adds inoculation by seeding tank stream, 10%, 30 ℃ of inoculum concentration, and 300rpm, 1: 0.4 volume ratio of ventilation was cultivated 8 hours; Improving ventilation again is 1: 0.6, cultivates 12 hours; Changing ventilation at last is 1: 0.8 volume ratio, and stream adds the silkworm chrysalis hydrolyzate fed-batch fermentation of 5% volume, cultivates 12～16 hours again.Zymotic fluid directly carries out spray-drying, makes bacillus subtilis complex enzyme pulvis.
(2) aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae)
A. culture medium
Seed culture medium: with wheat bran and water by etc. part by weight mix 121 ℃ of sterilizations 30 minutes thoroughly.
The solid fermentation culture medium: wheat bran 20%, dregs of beans 70%, rice bran 10% is 1: 1 a ratio poach in material-water ratio, 121 ℃ of sterilizations 30 minutes.
B. condition of culture
Seed culture: the bottled seed culture medium wet feed of 1000ml triangle 50g, insert the spore suspension (5.0 * 10 that the inclined-plane is made
7Individual/ml) 1.0ml, after mixing thoroughly, 30 ℃, leave standstill and cultivated 30 hours, middle every 12 hours button bottles 1 time.Aspergillus niger is consistent with the aspergillus oryzae condition of culture.
Mixing fermentation culture: get sterilization back solid fermentation material, in the process fermentation plant of disinfecting, open wide and to dry in the air when the material temperature is 40 ℃ of left and right sides, cultured aspergillus niger and aspergillus oryzae seed material are rubbed broken, respectively be spread in the fermentation material and mix inoculation thoroughly by 5% amount branch, inoculation after fermentation material is tiled on the fermentation bed, about 2～3 centimetres of thickness, the control temperature is 30 ℃, relative humidity is 85%～90%, when cultivation was cultured to 12 and 24 hours, each tilting table once; Be cultured to 30 hours, reduce room temperature to 25 ℃, cultivated again 24～30 hours; Get the aspergillus complex enzyme formulation after fermentation material results are air-dry.
3 preparation processing
Bacillus subtilis complex enzyme pulvis and aspergillus aspergillus complex enzyme formulation mix composite modulation according to 1: 1 part by weight, become the complex enzyme formulation that contains AMS, protease, cellulase and lipase isoreactivity.
(3) composite modulation
Active bacteria formulation and complex enzyme formulation are carried out composite according to 1: 4 proportion compatibility, make additive product after mixing.The every gram of product contains beneficial microbe viable count＞10
9Cfu, AMS is lived and to be every gram product per minute hydrolyzed starch amount＞4000 milligrams, protease activity is that every gram product per minute caseinhydrolysate generates amount of amino acid＞500 milligram, cellulase activity is every gram product per minute degraded cellulose amount＞200 milligrams, and lipase activity is every gram product per minute hydrolyzing triglyceride amount＞150 milligrams.
Advantage of the present invention be with this product as additive, possessed two kinds of effects of active bacteria formulation and enzyme preparation simultaneously, improve on the one hand the ruminant tumor gastric fermentation, increase feed intake, strengthen animal health condition, improve production performance; Improve efficiency of feed utilization on the other hand, improve the price of deed, reduce production costs; Can improve the economic benefit of breeding production from two aspects.After using product of the present invention in the actual production, can improve the feed intake of milk cow and, improve the calf daily gain and be up to 46.75%, reduce feedstuff-meat ratio and be up to 31.7% the utilization ratio of roughage, improve milk production of cow and reach 6.30%, significantly increase economic benefit.
Four specific embodiment
Embodiment 1.
In replacement cattle workshop, a milk cattle cultivating field, 40 of the holstein cows at 11～16 monthly ages of selection are divided into test group and control group by the randomized block experiment design.Daily ration consists of: 1.95 kilograms of corns, 0.3 kilogram of dregs of beans, 0.24 kilogram of cotton dregs, 0.39 kilogram in wheat bran, 10 kilograms of corn silages, 3 kilograms of brewex's grains, 0.12 kilogram of premix, hay free choice feeding, test group every day be divided into mixing thoroughly to add with fine fodder for 2 times with product 50 grams of the present invention and feed, control group does not add.Raise for hive off indefinite groove of examination Niu Caiyong, divide every day and go up the groove feeding three times, 7～8 hours at interval.
In 34 days experimental period, the feed intake and the grazing speed that add test ox behind the product of the present invention are normal, and the test group average daily gain is 1.13 kilograms, improved 46.75% than control group, feedstuff-meat ratio is 2.66, improves than control group and reduces by 31.7%, and concrete result of the test sees Table 1.The result that feeds shows that product of the present invention can improve digestibility and the utilization rate of calf to roughage, obviously increases daily gain, reduces feedstuff-meat ratio, improves the price of deed, increases economic benefit.
The test of table 1 growing cattle gaining effect
The test group control group
Ox number (head) 20 20
Experimental period (my god) 34 34
Initial average heavy (kilogram) 320.75 323.60
Average heavy (kilogram) 359.15 349.90 in test back
Average daily gain (kilogram) 1.13 0.77
Fine fodder: increased weight 2.66: 1 3.90: 1
Embodiment 2.
38 of healthy adult holstein cows are chosen in one-tenth cow workshop a milk cattle cultivating field, age-based, parity, the lactation moon, the principle that daily yielding is close, and pairing is divided into test group and control group, 19 every group.Daily ration consists of: 1.01 kilograms of corn stalk pieces, corn stalk powder 3.01,0.39 kilogram in sheep's hay, 13.79 kilograms of corn silages, 4.58 kilograms of brewex's grains, 0.22 kilogram of the dried dregs of rice, 1.8 kilograms in carrot, 9.76 kilograms of fine fodders, test group every day be divided into mixing thoroughly to add with fine fodder for 2 times with product 50 grams of the present invention and feed, control group does not add.Divide feeding three times every day for the examination ox, three times mechanical pipe is milked, 7～8 hours at interval.
Through test in 35 days, test group increased day by day and gives milk 1.79 kilograms, and control group increases day by day and gives milk 0.06 kilogram, and test group increases 1.73 kilograms of milk than control group every day more, amount of increase in production 6.30%, and the test group butterfat percnetage has a net increase of 0.11 percentage point than control group, and concrete data see Table 2.The result that feeds shows that product of the present invention can improve lumen fermentation, improves efficiency of feed utilization and conversion ratio, improves the lactating cow output of milk, improves butterfat percnetage, can increase the culturing economic benefit.
The table 2 cow in milk effect test of giving milk
The test group control group
Ox number (head) 19 19
Experimental period (my god) 35 35
Initial per day the giving milk (kilogram) 27.30 26.11 of the average daily output of milk
(kilogram) 29.09 26.17 of on average giving milk after the test
(kilogram) 1.79 0.06 of giving milk on average increases day by day
Amount of increase in production 6.3%
The initial per day butterfat percnetage of butterfat percnetage (%) 3.48 3.45
The average butterfat percnetage (%) 3.53 3.39 in test back
Butterfat percnetage has a net increase of percentage and counts 0.11
Claims (2)
1. the special-purpose economic benefits and social benefits microbe additive of a ruminant, it is characterized in that being prepared into the active bacteria formulation of lactobacillus acidophilus and saccharomyces cerevisiae culture by fermentation, with make complex enzyme formulation with liquid fermentation, aspergillus oryzae and aspergillus niger with solid mixed culture fermentation compatibility by bacillus subtilis, the composite in proportion economic benefits and social benefits feedstuff additive product of making.The every gram of this invention product contains beneficial microbe viable count＞10
9Cfu, AMS is lived and to be every gram product per minute hydrolyzed starch amount＞4000 milligrams, protease activity is that every gram product per minute caseinhydrolysate generates amount of amino acid＞500 milligram, cellulase activity is every gram product per minute degraded cellulose amount＞200 milligrams, and lipase activity is every gram product per minute hydrolyzing triglyceride amount＞150 milligrams.After using product of the present invention in the actual production, can improve the calf daily gain and be up to 46.75%, reduce feedstuff-meat ratio and be up to 31.7%, improve milk production of cow and reach 6.30%, significantly increase economic benefit.
2. the preparation method of the special-purpose economic benefits and social benefits microbe additive of a ruminant is characterized in that:
(1) active bacteria formulation preparation
1 bacterial classification
Lactobacillus acidophilus (Lactobacillus acidophilus), saccharomyces cerevisiae (Saccharomyce cerevisiae).
2 raw materials
After adopting fresh yellow maize to pulverize to handle, be that 1: 3.0～4.0 ratio adds 50 ℃ of warm water spices, be heated to 80 ℃ after mixing thoroughly and precooked 30～40 minutes, at 132～135 ℃, 2.8～3.0kg/cm in material-water ratio
2Boiling is 90 minutes under the condition, is cooled to about 65 ℃, adds high temperature Ye Huamei and carbohydrase and handles 3～4 hours, obtain the DE value and be more than 60%, pol is at the corn enzymatic saccharification liquid of 12～14 ° of Bx, regulating the pH value is about 4.5, is heated to about 90 ℃ to keep 30 minutes again, stand-by.
3 zymotechniques
(1) lactobacillus acidophilus (Lactobacillus acidophilus)
A. culture medium
Seed culture medium (g/1000ml): soy peptone 10g, beef extract 10g, yeast extract 5g, K
2HPO
42g, dibasic ammonium citrate 2g, sodium acetate 5g, glucose 20g, Tween 80 1mL, MgSO
47H
2O 0.58g, MnSO
44H
2O 0.25g, pH6.2 sterilized 20 minutes for 115 ℃.
Fermentation medium: corn enzymatic saccharification liquid adds 3% defatted soy flour, sterilizes 30 minutes for 121 ℃.
B. condition of culture
First order seed is cultivated: be shake flask fermentation, get the bottled seed culture medium 50ml of 250ml triangle, get slant activation bacterial classification 1 articulating and go in the culture medium, 30 ℃, the 220rpm rotary shaker was cultivated 36 hours.
Secondary seed is cultivated: the 10L automated seed canned seed culture medium 6L that ferments, by 5% inoculum concentration, 30 ℃, 300rpm cultivated 28 hours.
The full-automatic stirred fermentor dress of fermentation tank culture: 100L fermentation medium 60L adds inoculation by seeding tank stream, 10%, 30 ℃ of inoculum concentration, and 300rpm cultivated 28～32 hours.
(2) saccharomyces cerevisiae (Saccharomyce cerevisiae)
A. culture medium
Seed culture medium: get fresh moyashi and add water in 1: 10 ratio, boiled 30 minutes, use filtered through gauze, filtrate adds water and supplies initial volume, adds glucose 5%, and natural pH value was sterilized 30 minutes for 115 ℃.
Fermentation medium: corn enzymatic saccharification liquid.
B. condition of culture
First order seed is cultivated: be shake flask fermentation, get the bottled seed culture medium 50ml of 250ml triangle, get slant activation bacterial classification 1 articulating and go in the culture medium, 28 ℃, 260rpm cultivated 16 hours.
Secondary seed is cultivated: the 10L automated seed canned fermentation medium 6L that ferments, by 5% inoculum concentration, 28 ℃, 300rpm cultivated 18 hours.
The full-automatic stirred fermentor dress of fermentation tank culture: 100L fermentation medium 60L adds inoculation by seeding tank stream, 10%, 28 ℃ of inoculum concentration, and 200rpm cultivated 18～24 hours.
4 dry processing
Add in the zymotic fluid 0.5% whey powder as protective agent after, pass through spray-drying respectively, make lactobacillus acidophilus cultures (viable count＞1.5 * 10
9Cfu/g) and yeast culture (viable count＞1.0 * 10
10Cfu/g), the two is re-dubbed viable count in 40: 60 ratio and surpasses 6.6 * 10
9The active bacteria formulation of cfu/g.
(2) enzyme preparation
1 bacterial classification
Bacillus subtilis (Bacillus subtilis), aspergillus oryzae (Aspergillus oryzae) and aspergillus niger (Aspergillus niger).
2 zymotechniques
(1) bacillus subtilis (Bacillus subtilis)
A. culture medium
Seed culture medium: soya-bean cake powder 3%, corn flour 2%, wheat bran 3%, Na
2HPO
40.4%, KH
2PO
40.03%, natural pH value was sterilized 20 minutes for 121 ℃.
Fermentation medium: soya-bean cake powder 3%, corn flour 5%, wheat bran 4%, Na
2HPO
40.4%, KH
2PO
40.03%, natural pH value was sterilized 20 minutes for 121 ℃.
Feeding medium during fermentation liquid: get silkworm chrysalis: lime: water mixes, 1.0 * 10 in 100: 6: 600 ratio
5Heating hydrolysis is 1 hour under the Pa steam pressure, makes the silkworm chrysalis hydrolyzate, as feeding medium during fermentation liquid.
B. condition of culture
Seed culture: the 10L automated seed canned fermentation medium 6L that ferments, get the cell suspension (about 1.0 * 10 that slant strains is made
11Cfu/m1) insert seeding tank by 1% inoculum concentration, 30 ℃, 1: 1.2 volume ratio of ventilation, 350rpm cultivated 8 hours.
The full-automatic stirred fermentor dress of fermented and cultured: 100L fermentation medium 60L adds inoculation by seeding tank stream, 10%, 30 ℃ of inoculum concentration, and 300rpm, 1: 0.4 volume ratio of ventilation was cultivated 8 hours; Improving ventilation again is 1: 0.6, cultivates 12 hours; Changing ventilation at last is 1: 0.8 volume ratio, and stream adds the silkworm chrysalis hydrolyzate fed-batch fermentation of 5% volume, cultivates 12～16 hours again.Zymotic fluid directly carries out spray-drying, makes bacillus subtilis complex enzyme pulvis.
(2) aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae)
A. culture medium
Seed culture medium: with wheat bran and water by etc. part by weight mix 121 ℃ of sterilizations 30 minutes thoroughly.
The solid fermentation culture medium: wheat bran 20%, dregs of beans 70%, rice bran 10% is 1: 1 a ratio poach in material-water ratio, 121 ℃ of sterilizations 30 minutes.
B. condition of culture
Seed culture: the bottled seed culture medium wet feed of 1000ml triangle 50g, insert the spore suspension (5.0 * 10 that the inclined-plane is made
7Individual/ml) 1.0ml, after mixing thoroughly, 30 ℃, leave standstill and cultivated 30 hours, middle every 12 hours button bottles 1 time.Aspergillus niger is consistent with the aspergillus oryzae condition of culture.
Mixing fermentation culture: get sterilization back solid fermentation material, in the process fermentation plant of disinfecting, open wide and to dry in the air when the material temperature is 40 ℃ of left and right sides, cultured aspergillus niger and aspergillus oryzae seed material are rubbed broken, respectively be spread in the fermentation material and mix inoculation thoroughly by 5% amount branch, inoculation after fermentation material is tiled on the fermentation bed, about 2～3 centimetres of thickness, the control temperature is 30 ℃, relative humidity is 85%～90%, when cultivation was cultured to 12 and 24 hours, each tilting table once; Be cultured to 30 hours, reduce room temperature to 25 ℃, cultivated again 24～30 hours; Get the aspergillus complex enzyme formulation after fermentation material results are air-dry.
3 preparation processing
Bacillus subtilis complex enzyme pulvis and aspergillus aspergillus complex enzyme formulation mix composite modulation according to 1: 1 part by weight, become the complex enzyme formulation that contains AMS, protease, cellulase and lipase isoreactivity.
(3) composite modulation
Active bacteria formulation and complex enzyme formulation are carried out composite according to 1: 4 proportion compatibility, make additive product after mixing.The every gram of product contains beneficial microbe viable count＞10
9Cfu, AMS is lived and to be every gram product per minute hydrolyzed starch amount＞4000 milligrams, protease activity is that every gram product per minute caseinhydrolysate generates amount of amino acid＞500 milligram, cellulase activity is every gram product per minute degraded cellulose amount＞200 milligrams, and lipase activity is every gram product per minute hydrolyzing triglyceride amount＞150 milligrams.
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|---|---|---|---|
| CNB2004100045511A CN1283172C (en) | 2004-02-23 | 2004-02-23 | Two-effects microbiological additives of forage specially used for ruminants |
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|---|---|---|---|
| CNB2004100045511A CN1283172C (en) | 2004-02-23 | 2004-02-23 | Two-effects microbiological additives of forage specially used for ruminants |
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| Publication Number | Publication Date |
|---|---|
| CN1559261A true CN1559261A (en) | 2005-01-05 |
| CN1283172C CN1283172C (en) | 2006-11-08 |
Family
ID=34439606
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
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