CN105581023A - Microbial ferment feed additive conversion agent and application thereof - Google Patents
Microbial ferment feed additive conversion agent and application thereof Download PDFInfo
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- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 71
- 239000003674 animal food additive Substances 0.000 title abstract description 7
- 238000006243 chemical reaction Methods 0.000 title abstract 5
- 230000000813 microbial effect Effects 0.000 title abstract 5
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 47
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 47
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 45
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 45
- 241000186429 Propionibacterium Species 0.000 claims abstract description 44
- 240000006439 Aspergillus oryzae Species 0.000 claims abstract description 40
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims abstract description 40
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 32
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 32
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 32
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims abstract description 27
- 238000000855 fermentation Methods 0.000 claims abstract description 26
- 235000013336 milk Nutrition 0.000 claims abstract description 18
- 239000008267 milk Substances 0.000 claims abstract description 18
- 210000004080 milk Anatomy 0.000 claims abstract description 18
- 235000013601 eggs Nutrition 0.000 claims abstract description 6
- 235000013372 meat Nutrition 0.000 claims abstract description 4
- 238000010521 absorption reaction Methods 0.000 claims abstract description 3
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 claims abstract 8
- 235000019260 propionic acid Nutrition 0.000 claims abstract 4
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 claims abstract 4
- 230000001131 transforming effect Effects 0.000 claims description 65
- 102000004190 Enzymes Human genes 0.000 claims description 61
- 108090000790 Enzymes Proteins 0.000 claims description 61
- 229940088598 enzyme Drugs 0.000 claims description 61
- 108091005804 Peptidases Proteins 0.000 claims description 39
- 239000004365 Protease Substances 0.000 claims description 39
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 39
- 108010059892 Cellulase Proteins 0.000 claims description 36
- 229940106157 cellulase Drugs 0.000 claims description 36
- 241000894006 Bacteria Species 0.000 claims description 29
- 239000002131 composite material Substances 0.000 claims description 26
- 239000007788 liquid Substances 0.000 claims description 26
- 239000002994 raw material Substances 0.000 claims description 20
- 235000013379 molasses Nutrition 0.000 claims description 14
- 240000008042 Zea mays Species 0.000 claims description 10
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 10
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 10
- 235000005822 corn Nutrition 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 9
- 239000010902 straw Substances 0.000 claims description 9
- 235000013312 flour Nutrition 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 7
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 7
- 235000015099 wheat brans Nutrition 0.000 claims description 7
- 241000589516 Pseudomonas Species 0.000 claims description 6
- 230000009286 beneficial effect Effects 0.000 claims description 4
- 208000035240 Disease Resistance Diseases 0.000 claims description 2
- 241000235070 Saccharomyces Species 0.000 claims description 2
- 230000001079 digestive effect Effects 0.000 claims description 2
- 244000052616 bacterial pathogen Species 0.000 claims 1
- 241001465754 Metazoa Species 0.000 abstract description 9
- 230000004151 fermentation Effects 0.000 abstract description 9
- 201000010099 disease Diseases 0.000 abstract description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 7
- 230000007423 decrease Effects 0.000 abstract description 3
- 239000001913 cellulose Substances 0.000 abstract description 2
- 229920002678 cellulose Polymers 0.000 abstract description 2
- 230000036039 immunity Effects 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 230000029087 digestion Effects 0.000 abstract 1
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- 241000283690 Bos taurus Species 0.000 description 17
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- 230000037396 body weight Effects 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 241000287828 Gallus gallus Species 0.000 description 4
- 239000004459 forage Substances 0.000 description 4
- 230000002265 prevention Effects 0.000 description 4
- 208000024891 symptom Diseases 0.000 description 4
- 206010000060 Abdominal distension Diseases 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
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- 244000025254 Cannabis sativa Species 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
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- 239000000575 pesticide Substances 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 206010034018 Parosmia Diseases 0.000 description 1
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- 150000001413 amino acids Chemical class 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
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- 235000019784 crude fat Nutrition 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/61—Propionibacterium
- A23V2400/613—Acidipropionici
Landscapes
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a microbial ferment feed additive conversion agent and an application thereof and belongs to the technical field of feed additives. The microbial ferment feed additive conversion agent comprises Aspergillus oryzae, propionic acid producing Propionibacterium, rhodopseudomonas palustris, bacillus subtilis, saccharomyces cerevisiae and Lactobacillus acidophilus. The microbial ferment feed additive conversion agent can effectively increase yields of meat, eggs and milk, play roles in preventing and inhibiting diseases and enhancing immunity and increase the absorption and digestion utilization rate of feed by animals; after fermentation, the microbial ferment feed additive conversion agent can effectively increase nutrient substances of the feed, increase the protein content, reduce the cellulose content and decrease the number of moulds.
Description
Technical field
The present invention relates to a kind of enzyme microb feed and add transforming agent and application thereof, belong to field of feed additive technology.
Background technology
At present forage grass, the feed grain of China's cultivated animals are mainly still to utilize chemical fertilizer, pesticide producing agricultural product out, due to greatlyAmount is used the residual product quality problem that just brings many cultivated animals of fertile medicine of fertilizer and pesticide, crop products, cultivated animalsOwn body, disease-resistant and assimilate function and can not sit on the sidelines too, therefore, our animal products quality exists for many yearsThe problem of decline, epidemic prevention cost polluting at rising, environment and water also can not be ignored. Use beneficial microbe and ferment product,To solve an as above effective way of problem. But owing to being subject to the restriction of the factors such as production technology and pursuit interests, on marketMicrobial bacterial agent product often bacterial classification is single, and poor activity, even if the U.S. exists bacterial classification list too with the import bacteria preparation of sending outOne problem, comprises Japanese EM, due to two during the last ten years, the stagnation of operation technique, occurred many unsatisfactory, asThe problem that product yield reduces although product protection effect is good. Therefore, select a good microorganism combination, and attached itWith ferment effect, make it with real accessing by product, realize quality and rise, the healthy existence of cultivated animals and improvement environmentThe object of polluting, the present invention has analysed diseases prevention scientifically and has pressed down the assimilating of sick, cultivated animals, feed-use grass, residual with the fertile medicine of grainStay processing and lignin thereof, coarse-fibred degraded, the problems such as the smell change of oil extraction thing, and by the effect of fermentation technique,Solve to greatest extent the problems referred to above, had very important significance.
Summary of the invention
For solving the deficiencies in the prior art, the invention provides a kind of enzyme microb feed and add transforming agent and application thereof, adoptTechnical scheme as follows:
The object of the present invention is to provide a kind of enzyme microb feed to add transforming agent, comprise following active component: aspergillus oryzae,Produce propionibacterium acide-propionici, Rhodopseudomonas palustris, bacillus subtilis, saccharomyces cerevisiae, lactobacillus acidophilus.
Preferably, described enzyme microb feed adds transforming agent, also contains protease and cellulase.
More preferably, the addition of described protease is 0.001mg/L-0.1mg/L; The addition of described cellulase is0.001mg/L-0.1mg/L。
Preferably, described aspergillus oryzae, produces propionibacterium acide-propionici, and the concentration of Rhodopseudomonas palustris and bacillus subtilis is1.0×108CFU/mL-9×108CFU/mL; Described saccharomyces cerevisiae and lactobacillus acidophilus concentration are 1.0 × 108CFU/mL-8.5×108CFU/mL。
More preferably, described aspergillus oryzae, produces propionibacterium acide-propionici, and the concentration of Rhodopseudomonas palustris and bacillus subtilis is5.0×108CFU/mL-9.0×108CFU/mL; Described saccharomyces cerevisiae and lactobacillus acidophilus concentration are 4.8 × 108CFU/mL–8.5×108CFU/mL。
The above arbitrary enzyme microb feed adds transforming agent in the application of preparing in biological feedstuff.
Preferably, described application, step is as follows:
1) cultivate respectively aspergillus oryzae, produce propionibacterium acide-propionici, Rhodopseudomonas palustris, bacillus subtilis, saccharomyces cerevisiae and have a liking for acidLactobacillus to logarithmic phase obtains bacterium liquid, each bacterium liquid is carried out composite, makes aspergillus oryzae after composite, produces propionibacterium acide-propionici, natural pondThe concentration of pool red pseudomonas and bacillus subtilis is 1.0 × 108CFU/mL-9×108CFU/mL, saccharomyces cerevisiae andLactobacillus acidophilus concentration is 1.0 × 108CFU/mL-8.5×108CFU/mL, obtains enzyme microb feed and adds conversionAgent;
2) taking one or more in powder of straw, corn flour, dregs of beans, wheat bran, oily chaff and rape cake as raw material, in 100 parts of raw materialsAdding 0.3 part of-1.5 parts of step 1) the enzyme microb feed that obtains adds transforming agent, and then adds 0.3 part of-1.5 parts of molasses,At 18 DEG C-40 DEG C, carry out anaerobic fermentation.
Preferably, described molasses are identical with enzyme microb feed interpolation transforming agent addition.
Preferably, described application, step is as follows:
1) cultivate respectively aspergillus oryzae, produce propionibacterium acide-propionici, Rhodopseudomonas palustris, bacillus subtilis, saccharomyces cerevisiae and have a liking for acidLactobacillus to logarithmic phase obtains bacterium liquid, each bacterium liquid is carried out composite, makes aspergillus oryzae after composite, produces propionibacterium acide-propionici, natural pondThe concentration of pool red pseudomonas and bacillus subtilis is 1.0 × 108CFU/mL-9×108CFU/mL, saccharomyces cerevisiaeWith lactobacillus acidophilus concentration be 1.0 × 108CFU/mL-8.5×108CFU/mL, then adds protease and cellulaseMake the concentration of protease and cellulase be 0.001mg/L-0.1mg/L, obtain enzyme microb feed and add transforming agent;
2) taking one or more in powder of straw, corn flour, dregs of beans, wheat bran, oily chaff and rape cake as raw material, in 100 parts of raw materialsAdd 0.3 part of-1.5 parts of step 1) obtain enzyme microb feed add transforming agent, 0.3 part of-1.5 parts of molasses, at 18 DEG C-40 DEG CUnder carry out anaerobic fermentation.
More preferably, described application, concrete steps are as follows:
1) cultivate respectively aspergillus oryzae, produce propionibacterium acide-propionici, Rhodopseudomonas palustris, bacillus subtilis, saccharomyces cerevisiae and have a liking for acidLactobacillus to logarithmic phase obtains bacterium liquid, each bacterium liquid is carried out composite, makes aspergillus oryzae after composite, produces propionibacterium acide-propionici, natural pondThe concentration of pool red pseudomonas and bacillus subtilis is 5.0 × 108CFU/mL, saccharomyces cerevisiae and lactobacillus acidophilus concentrationBe 4.8 × 108CFU/mL, then adds protease and cellulase that the concentration of protease and cellulase is0.05mg/L, obtains enzyme microb feed and adds transforming agent;
2) taking one or more in powder of straw, corn flour, dregs of beans, wheat bran, oily chaff and rape cake as raw material, in 100 parts of raw materialsAdding 1 part of step 1) the enzyme microb feed that obtains adds transforming agent, and then adds 1 part of molasses, at 18 DEG C-40 DEG CCarry out anaerobic fermentation.
Biological feedstuff prepared by the above either method improving meat, eggs and milk output, improve cultivated animals disease resistance ability andApplication in abilities of digestive and absorption, raising fermented feed beneficial bacterium viable count.
Beneficial effect of the present invention:
Microorganism teaching feed provided by the invention adds transforming agent, can effectively improve the output of meat, eggs and milk, can also play anti-Sick, press down effect sick, that improve immunity, improve the assimilate utilization rate of animal to feed, by can be effective after fermentationImprove the nutriment of feed, improve protein content, reduce content of cellulose, and reduce mould quantity.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention will be further described, but the present invention is not subject to the restriction of embodiment.
Embodiment 1:
The present embodiment provides a kind of enzyme microb feed to add transforming agent, comprises aspergillus oryzae, produces propionibacterium acide-propionici, and marsh is redPseudomonad, bacillus subtilis, saccharomyces cerevisiae, lactobacillus acidophilus, also contains protease and cellulase, wherein: meter QuMould, produce propionibacterium acide-propionici, the concentration of Rhodopseudomonas palustris and bacillus subtilis is 5.0 × 108CFU/mL; Described wineBrewer yeast and lactobacillus acidophilus concentration are 4.8 × 108CFU/mL。
Cultivate respectively aspergillus oryzae, produce propionibacterium acide-propionici, Rhodopseudomonas palustris, bacillus subtilis, saccharomyces cerevisiae and have a liking for acidLactobacillus to logarithmic phase obtains bacterium liquid, each bacterium liquid is carried out composite, makes aspergillus oryzae after composite, produces propionibacterium acide-propionici, and marsh is redThe concentration of pseudomonad and bacillus subtilis is 5.0 × 108CFU/mL, saccharomyces cerevisiae and lactobacillus acidophilus concentration are4.8×108CFU/mL, obtains enzyme microb feed and adds transforming agent; Taking powder of straw as raw material, in 100 parts of raw materials, add 1Part enzyme microb feed adds transforming agent, and then adds 1 part of molasses, at 18 DEG C-40 DEG C, carries out anaerobic fermentation.
Embodiment 2:
The present embodiment provides a kind of enzyme microb feed to add transforming agent, comprises aspergillus oryzae, produces propionibacterium acide-propionici, and marsh is redPseudomonad, bacillus subtilis, saccharomyces cerevisiae, lactobacillus acidophilus, also contains protease and cellulase, wherein: meter QuMould, produce propionibacterium acide-propionici, the concentration of Rhodopseudomonas palustris and bacillus subtilis is 9.0 × 108CFU/mL; Described wineBrewer yeast and lactobacillus acidophilus concentration are 8.5 × 108CFU/mL。
Cultivate respectively aspergillus oryzae, produce propionibacterium acide-propionici, Rhodopseudomonas palustris, bacillus subtilis, saccharomyces cerevisiae and have a liking for acidLactobacillus to logarithmic phase obtains bacterium liquid, each bacterium liquid is carried out composite, makes aspergillus oryzae after composite, produces propionibacterium acide-propionici, and marsh is redThe concentration of pseudomonad and bacillus subtilis is 9.0 × 108CFU/mL, saccharomyces cerevisiae and lactobacillus acidophilus concentration are8.5×108CFU/mL, obtains enzyme microb feed and adds transforming agent; Taking corn flour as raw material, in 100 parts of raw materials, add0.3 part of enzyme microb feed adds transforming agent, and then adds 0.3 part of molasses, at 18 DEG C-40 DEG C, carries out anaerobic fermentation.
Embodiment 3:
The present embodiment provides a kind of enzyme microb feed to add transforming agent, comprises aspergillus oryzae, produces propionibacterium acide-propionici, and marsh is redPseudomonad, bacillus subtilis, saccharomyces cerevisiae, lactobacillus acidophilus, also contains protease and cellulase, wherein: meter QuMould, produce propionibacterium acide-propionici, the concentration of Rhodopseudomonas palustris and bacillus subtilis is 1.0 × 108CFU/mL; Described wineBrewer yeast and lactobacillus acidophilus concentration are 1.0 × 108CFU/mL。
Cultivate respectively aspergillus oryzae, produce propionibacterium acide-propionici, Rhodopseudomonas palustris, bacillus subtilis, saccharomyces cerevisiae and have a liking for acidLactobacillus to logarithmic phase obtains bacterium liquid, each bacterium liquid is carried out composite, makes aspergillus oryzae after composite, produces propionibacterium acide-propionici, and marsh is redThe concentration of pseudomonad and bacillus subtilis is 1.0 × 108CFU/mL, saccharomyces cerevisiae and lactobacillus acidophilus concentration are1.0×108CFU/mL, obtains enzyme microb feed and adds transforming agent; Taking oily chaff as raw material, in 100 parts of raw materials, add 1.5Part enzyme microb feed adds transforming agent, and then adds 1.5 parts of molasses, at 18 DEG C-40 DEG C, carries out anaerobic fermentation.
Embodiment 4:
The present embodiment provides a kind of enzyme microb feed to add transforming agent, comprises aspergillus oryzae, produces propionibacterium acide-propionici, and marsh is redPseudomonad, bacillus subtilis, saccharomyces cerevisiae, lactobacillus acidophilus, also contains protease and cellulase, wherein: meter QuMould, produce propionibacterium acide-propionici, the concentration of Rhodopseudomonas palustris and bacillus subtilis is 5.0 × 108CFU/mL; Described wineBrewer yeast and lactobacillus acidophilus concentration are 4.8 × 108CFU/mL; The addition of protease and cellulase is 0.05mg/L.
Cultivate respectively aspergillus oryzae, produce propionibacterium acide-propionici, Rhodopseudomonas palustris, bacillus subtilis, saccharomyces cerevisiae and have a liking for acidLactobacillus to logarithmic phase obtains bacterium liquid, each bacterium liquid is carried out composite, makes aspergillus oryzae after composite, produces propionibacterium acide-propionici, and marsh is redThe concentration of pseudomonad and bacillus subtilis is 5.0 × 108CFU/mL, saccharomyces cerevisiae and lactobacillus acidophilus concentration are4.8×108CFU/mL, then adds protease and cellulase to make the concentration of protease and cellulase be 0.05mg/L, obtainsObtain enzyme microb feed and add transforming agent; Taking powder of straw as raw material, in 100 parts of raw materials, add 1 part of enzyme microb feed to addAdd transforming agent, and then add 1 part of molasses, at 18 DEG C-40 DEG C, carry out anaerobic fermentation.
Embodiment 5:
The present embodiment provides a kind of enzyme microb feed to add transforming agent, comprises aspergillus oryzae, produces propionibacterium acide-propionici, and marsh is redPseudomonad, bacillus subtilis, saccharomyces cerevisiae, lactobacillus acidophilus, also contains protease and cellulase, wherein: meter QuMould, produce propionibacterium acide-propionici, the concentration of Rhodopseudomonas palustris and bacillus subtilis is 9.0 × 108CFU/mL; Described wineBrewer yeast and lactobacillus acidophilus concentration are 8.5 × 108CFU/mL; The addition of protease and cellulase is 0.001mg/L.
Cultivate respectively aspergillus oryzae, produce propionibacterium acide-propionici, Rhodopseudomonas palustris, bacillus subtilis, saccharomyces cerevisiae and have a liking for acidLactobacillus to logarithmic phase obtains bacterium liquid, each bacterium liquid is carried out composite, makes aspergillus oryzae after composite, produces propionibacterium acide-propionici, and marsh is redThe concentration of pseudomonad and bacillus subtilis is 9.0 × 108CFU/mL, saccharomyces cerevisiae and lactobacillus acidophilus concentration are8.5×108CFU/mL, then adds protease and cellulase to make the concentration of protease and cellulase be 0.001mg/L,Obtain enzyme microb feed and add transforming agent; Taking corn flour as raw material, in 100 parts of raw materials, add 0.3 part of enzyme microb to raiseMaterial adds transforming agent, and then adds 0.3 part of molasses, at 18 DEG C-40 DEG C, carries out anaerobic fermentation.
Embodiment 6:
The present embodiment provides a kind of enzyme microb feed to add transforming agent, comprises aspergillus oryzae, produces propionibacterium acide-propionici, and marsh is redPseudomonad, bacillus subtilis, saccharomyces cerevisiae, lactobacillus acidophilus, also contains protease and cellulase, wherein: meter QuMould, produce propionibacterium acide-propionici, the concentration of Rhodopseudomonas palustris and bacillus subtilis is 1.0 × 108CFU/mL; Described wineBrewer yeast and lactobacillus acidophilus concentration are 1.0 × 108CFU/mL; The addition of protease and cellulase is 0.1mg/L.
Cultivate respectively aspergillus oryzae, produce propionibacterium acide-propionici, Rhodopseudomonas palustris, bacillus subtilis, saccharomyces cerevisiae and have a liking for acidLactobacillus to logarithmic phase obtains bacterium liquid, each bacterium liquid is carried out composite, makes aspergillus oryzae after composite, produces propionibacterium acide-propionici, and marsh is redThe concentration of pseudomonad and bacillus subtilis is 1.0 × 108CFU/mL, saccharomyces cerevisiae and lactobacillus acidophilus concentration are1.0×108CFU/mL, then adds protease and cellulase to make the concentration of protease and cellulase be 0.1mg/L, obtainsObtain enzyme microb feed and add transforming agent; Taking oily chaff as raw material, in 100 parts of raw materials, add 1.5 parts of enzyme microb feeds to addAdd transforming agent, and then add 1.5 parts of molasses, at 18 DEG C-40 DEG C, carry out anaerobic fermentation.
Embodiment 7:
The present embodiment provides a kind of enzyme microb feed to add transforming agent, comprises aspergillus oryzae, produces propionibacterium acide-propionici, and marsh is redPseudomonad, bacillus subtilis, saccharomyces cerevisiae, lactobacillus acidophilus, also contains protease and cellulase, wherein: meter QuMould, produce propionibacterium acide-propionici, the concentration of Rhodopseudomonas palustris and bacillus subtilis is 5.0 × 108CFU/mL; Described wineBrewer yeast and lactobacillus acidophilus concentration are 4.8 × 108CFU/mL; The addition of protease and cellulase is 0.05mg/L.
Cultivate respectively aspergillus oryzae, produce propionibacterium acide-propionici, Rhodopseudomonas palustris, bacillus subtilis, saccharomyces cerevisiae and have a liking for acidLactobacillus to logarithmic phase obtains bacterium liquid, each bacterium liquid is carried out composite, makes aspergillus oryzae after composite, produces propionibacterium acide-propionici, and marsh is redThe concentration of pseudomonad and bacillus subtilis is 5.0 × 108CFU/mL, saccharomyces cerevisiae and lactobacillus acidophilus concentration are4.8×108CFU/mL, then adds protease and cellulase to make the concentration of protease and cellulase be 0.05mg/L, obtainsObtain enzyme microb feed and add transforming agent; Taking rape cake, dregs of beans and wheat bran as raw material, to add in 100 parts of raw materials 0.8 part micro-Biologic ferment feed adds transforming agent, and then adds 0.8 part of molasses, at 18 DEG C-40 DEG C, carries out anaerobic fermentation.
Embodiment 8: effect embodiment
Prepare respectively enzyme microb feed according to the preparation method of embodiment 1 and embodiment 4 and add transforming agent, powder of straw is sent outNutriment before and after ferment detects, and result is as shown in table 1.
Table 1 nutrition content
Known by table 1 data, the application of the invention enzyme microb feed can obviously improve slightly after adding transforming agent fermentationThe content of albumen and crude fat, effectively reduces the content of crude fibre and fungi count. Meanwhile, by comparing embodiment 1 and embodiment4 data can know, the effect of adding cellulase and protease is better than un-added effect.
Prepare respectively enzyme microb feed according to the preparation method of embodiment 3 and embodiment 6 and add transforming agent, to oily chaff fermentationThe nutriment of front and back detects, and result is as shown in table 2.
Table 2 nutrition content
Known by table 2 data, the application of the invention enzyme microb feed can obviously improve egg after adding transforming agent fermentationWhite matter content and amino acid, fat content also increases, and effectively reduces the content of crude fibre and ash content. Meanwhile, by comparingThe data of embodiment 3 and embodiment 6 can know, the effect of adding cellulase and protease is better than un-added effect.
Embodiment 9
Prepare enzyme microb feed according to the method for embodiment 1-7 and add transforming agent, and carry out anaerobic fermentation acquisition fermented feed,Fermented feed is fed to 80 cow heads, each experimental group 10 cow heads, the output of milk of milk cow is at 50 jin ± 2 jin, by groupClosing and making every group of average output of milk of milk cow is 50 jin. The every cow head 2.3 jin of fermented feeds of feeding every day, feed 20 days continuously, noteThe total output of every day after record Cow-feeding fermented feed. Gather as shown in table 3. Control group is set simultaneously, control group Cow-feedingAdd the feed of transforming agent feeds without enzyme microb feed. Experimental period is: November～12 in winter in Harbin, HeilungkiangMonth.
Table 3 milk cow daily milk yield
More than test in the November～December in winter in Harbin, Heilungkiang and carry out, normal milk production of cow along with turning of weather cold and underFall, add transforming agent fermented feed by the enzyme microb that uses the present embodiment, find not turning with weather of milk production of cowCold and decline, the output of milk increases to some extent on the contrary. Milk production of cow starts from feeding within 2nd～3 days, to start significantly to increase, a Zhou HoupingAll increase production 4.2 jin. Experimental group reaches 19.7% than the highest raising of the control group output of milk. When within 20 days, experiment finishes, the output of milk is protectedKeep steady fixed.
Embodiment 9
Prepare enzyme microb feed according to the method for embodiment 1-7 and add transforming agent, and carry out anaerobic fermentation acquisition fermented feed,Fermented feed is fed to beef cattle, and each experimental group arranges 10 beef cattles, and the body weight of every beef cattle is at 500kg～600kg, every headThe milk cow 5 jin of fermented feeds of feeding every day, feed 20 days continuously, and beef cattle weight records the result control group that simultaneously arranges as shown in table 4,The feed without the fermentation of corresponding microorganism ferment feed interpolation transforming agent of control group fed equivalent.
Table 4 beef cattle growth after 20d of feeding
Find through experiment, after the forage feed beef cattle with enzyme microb interpolation transforming agent fermentation preparation of the present invention, can make beef cattleBody weight significantly increase, apparently higher than control group, body weight is the highest to be improved up to 18kg, a 7.4kg left side and control group only can increase weightThe right side, the present invention has more 10kg left and right than control group, is the twice of control group. Add the effect of protease and cellulase simultaneouslyFruit is better than the effect of not adding protease and cellulase.
Embodiment 10
Prepare enzyme microb feed according to the method for embodiment 1-7 and add transforming agent, and carry out anaerobic fermentation acquisition fermented feed,The corn feed of fermented feed alternative 30% is fed to pig, and each experimental group arranges 10 pigs, the about 40kg of body weight of every pigLeft and right. Control group is set simultaneously, the feed without the fermentation of corresponding microorganism ferment feed interpolation transforming agent of control group fed equivalent.It is as shown in table 5 that body weight records result.
Table 5 beef cattle weight
Find through experiment, after the forage feed pig with enzyme microb interpolation transforming agent fermentation preparation of the present invention, can make the body of pigRepresentation work increases, and apparently higher than control group, body weight gain rate can improve up to 127.79%-130.35%, increases than control groupLong rate improves 9.19%～11.75%, and the effect of simultaneously adding protease and cellulase is better than does not add protease and cellulaseEffect.
Embodiment 11
Prepare enzyme microb feed according to the method for embodiment 1-7 and add transforming agent, and carry out anaerobic fermentation acquisition fermented feed,Fermented feed is substituted to 30% corn feed and feed to chicken, it is the chicken of 21 weeks that each experimental group arranges 50 all ages, feed toWeek is 27 weeks age, every chicken 100g feed of feeding. Control group is set simultaneously, control group fed equivalent without corresponding microorganismFerment feed adds the feed of transforming agent fermentation. Record result as shown in table 6.
Table 6 statistics
Find through experiment, after the forage feed chicken with enzyme microb interpolation transforming agent fermentation preparation of the present invention, can make the product of chickenEgg amount significantly increases, and apparently higher than control group, laying rate can improve 8%-20% with respect to all egg productions of control group, adds simultaneouslyThe effect of protease and cellulase is better than the effect of not adding protease and cellulase.
Embodiment 12
The diseases prevention of adding transforming agent for explanation enzyme microb feed of the present invention presses down disease effect, and the present embodiment has carried out following experiment.According to the formula of embodiment 4 and preparation method ferment obtain fermented feed, by fermented feed feed to 3 headbands have abdominal distension and3 headbands have the milk cow that ruminates slow symptom, record symptom as shown in table 7 after feeding. Control group is set simultaneously, and control group is respectivelyTo with abdominal distension and ruminate slow symptom Cow-feeding equivalent add raising of transforming agent fermentation without corresponding microorganism ferment feedMaterial.
Table 7 pathography result
Known by above-mentioned experiment, add fermented feed prepared by transforming agent with enzyme microb feed of the present invention and can alleviate cultivationThe abdominal distension of animal and the symptom of not ruminating, enzyme microb feed of the present invention adds transforming agent can play the effect that diseases prevention presses down disease.
Although the present invention with preferred embodiment openly as above, it is not in order to limit the present invention, is anyly familiar with this technologyPeople, without departing from the spirit and scope of the present invention, can do various changes and modification, and therefore protection scope of the present invention shouldShould be with being as the criterion that claims were defined.
Claims (10)
1. enzyme microb feed adds a transforming agent, it is characterized in that, comprises following active component: aspergillus oryzae, produces propionic acid propionic acidBacillus, Rhodopseudomonas palustris, bacillus subtilis, saccharomyces cerevisiae, lactobacillus acidophilus.
2. enzyme microb feed adds transforming agent according to claim 1, it is characterized in that, also contains protease and cellulase.
3. enzyme microb feed adds transforming agent according to claim 2, it is characterized in that, the addition of described protease is0.001mg/L-0.1mg/L; The addition of described cellulase is 0.001mg/L-0.1mg/L.
4. add transforming agent according to enzyme microb feed described in claim 1 or 2, it is characterized in that, described aspergillus oryzae, produces propionic acid thirdAcidfast bacilli, the concentration of Rhodopseudomonas palustris and bacillus subtilis is 1.0 X 108CFU/mL-9ⅹ108CFU/mL；Described saccharomyces cerevisiae and lactobacillus acidophilus concentration are 1.0 X 108CFU/mL-8.5ⅹ108CFU/mL。
5. enzyme microb feed adds transforming agent according to claim 4, it is characterized in that described aspergillus oryzae produces propionic acid propionic acidBacillus, the concentration of Rhodopseudomonas palustris and bacillus subtilis is 5.0 X 108CFU/mL-9.0ⅹ108CFU/mL；Described saccharomyces cerevisiae and lactobacillus acidophilus concentration are 4.8 X 108CFU/mL–8.5ⅹ108CFU/mL。
6. described in claim 1-5, arbitrary enzyme microb feed adds transforming agent in the application of preparing in biological feedstuff.
7. application according to claim 6, is characterized in that, step is as follows:
1) cultivate respectively aspergillus oryzae, produce propionibacterium acide-propionici, Rhodopseudomonas palustris, bacillus subtilis, saccharomyces cerevisiae and have a liking for acidLactobacillus to logarithmic phase obtains bacterium liquid, each bacterium liquid is carried out composite, makes aspergillus oryzae after composite, produces propionibacterium acide-propionici, natural pondThe concentration of pool red pseudomonas and bacillus subtilis is 1.0 X 108CFU/mL-9ⅹ108CFU/mL, saccharomyces cerevisiae andLactobacillus acidophilus concentration is 1.0 X 108CFU/mL-8.5ⅹ108CFU/mL, obtains enzyme microb feed and adds conversionAgent;
2) taking one or more in powder of straw, corn flour, dregs of beans, wheat bran, oily chaff and rape cake as raw material, in 100 parts of raw materialsAdding 0.3 part of-1.5 parts of step 1) the enzyme microb feed that obtains adds transforming agent, and then adds 0.3 part of-1.5 parts of molasses,At 18 DEG C-40 DEG C, carry out anaerobic fermentation.
8. application according to claim 6, is characterized in that, step is as follows:
1) cultivate respectively aspergillus oryzae, produce propionibacterium acide-propionici, Rhodopseudomonas palustris, bacillus subtilis, saccharomyces cerevisiae and have a liking for acidLactobacillus to logarithmic phase obtains bacterium liquid, each bacterium liquid is carried out composite, makes aspergillus oryzae after composite, produces propionibacterium acide-propionici, natural pondThe concentration of pool red pseudomonas and bacillus subtilis is 1.0 X 108CFU/mL-9ⅹ108CFU/mL, saccharomyces cerevisiaeWith lactobacillus acidophilus concentration be 1.0 X 108CFU/mL-8.5ⅹ108CFU/mL, then adds protease and cellulaseMake the concentration of protease and cellulase be 0.001mg/L-0.1mg/L, obtain enzyme microb feed and add transforming agent;
2) taking one or more in powder of straw, corn flour, dregs of beans, wheat bran, oily chaff and rape cake as raw material, in 100 parts of raw materialsAdding 0.3 part of-1.5 parts of step 1) the enzyme microb feed that obtains adds transforming agent, and then adds 0.3 part of-1.5 parts of molasses,At 18 DEG C-40 DEG C, carry out anaerobic fermentation.
9. application according to claim 8, is characterized in that, concrete steps are as follows:
1) cultivate respectively aspergillus oryzae, produce propionibacterium acide-propionici, Rhodopseudomonas palustris, bacillus subtilis, saccharomyces cerevisiae and have a liking for acidLactobacillus to logarithmic phase obtains bacterium liquid, each bacterium liquid is carried out composite, makes aspergillus oryzae after composite, produces propionibacterium acide-propionici, natural pondThe concentration of pool red pseudomonas and bacillus subtilis is 5.0 X 108CFU/mL, saccharomyces cerevisiae and lactobacillus acidophilus concentrationBe 4.8 X 108CFU/mL, then adds protease and cellulase that the concentration of protease and cellulase is0.05mg/L, obtains enzyme microb feed and adds transforming agent;
2) taking one or more in powder of straw, corn flour, dregs of beans, wheat bran, oily chaff and rape cake as raw material, in 100 parts of raw materialsAdding 1 part of step 1) the enzyme microb feed that obtains adds transforming agent, and then adds 1 part of molasses, at 18 DEG C-40 DEG CCarry out anaerobic fermentation.
10. the biological feedstuff that described in claim 7-9 prepared by either method is improving the disease resistance of meat, eggs and milk output, raising cultivated animalsApplication in ability and abilities of digestive and absorption, raising fermented feed beneficial bacterium viable count.
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| CN107212166A (en) * | 2017-07-24 | 2017-09-29 | 新疆生产建设兵团第十师畜牧兽医站 | Beef cattle biological feedstuff and its method based on sunflower straw/sunflower seed shell |
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