CN110179015A - A kind of high-survival rate fermented shrimp material and preparation method thereof - Google Patents
A kind of high-survival rate fermented shrimp material and preparation method thereof Download PDFInfo
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- CN110179015A CN110179015A CN201910570482.7A CN201910570482A CN110179015A CN 110179015 A CN110179015 A CN 110179015A CN 201910570482 A CN201910570482 A CN 201910570482A CN 110179015 A CN110179015 A CN 110179015A
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- 229940035034 maltodextrin Drugs 0.000 claims abstract description 12
- 235000019733 Fish meal Nutrition 0.000 claims abstract description 11
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 11
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 11
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- 239000001257 hydrogen Substances 0.000 description 3
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- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
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- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 2
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- SFMJNHNUOVADRW-UHFFFAOYSA-N n-[5-[9-[4-(methanesulfonamido)phenyl]-2-oxobenzo[h][1,6]naphthyridin-1-yl]-2-methylphenyl]prop-2-enamide Chemical compound C1=C(NC(=O)C=C)C(C)=CC=C1N1C(=O)C=CC2=C1C1=CC(C=3C=CC(NS(C)(=O)=O)=CC=3)=CC=C1N=C2 SFMJNHNUOVADRW-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/173—Reuteri
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a kind of high-survival rate fermented shrimp material and preparation method thereof, the high-survival rate fermented shrimp material includes following substance: fish meal, dregs of beans, wheat bran, cornstarch, flour, black soldier flies slurry, maltodextrin, sodium alginate, the sub- liquid of activated spawn, raw material of the present invention cures in advance, fermentation in advance, make shrimp material raw material predigestion, fermentating metabolism generates a large amount of antibacterial substance simultaneously, lactic acid, digestive ferment etc., the utilization rate of feed can be improved, inhibit the breeding of pathogen in shrimp enteron aisle, repair shrimp enteron aisle, enhance the anti-ability of shrimp, reduce antibiotic usage, improve the unique effects such as survival rate.
Description
Technical field
The present invention relates to field of fodder, and in particular to a kind of high-survival rate fermented shrimp material and preparation method thereof.
Background technique
WithCultivationThe fast development of industry, scale livestock farming gradually increase cultivation density, necessarily lead to material culture in this way
Enteron aisle it is unhealthy, digestion and absorption goes wrong.It is well known that biofermentationFeedContaining profitable strain needed for animal,
Gradually received by raiser.By adding biofermentation material, is conducive to the maintenance of material culture enteron aisle, increases profitable strain and promote
The digestion and absorption of material culture, to adapt to following cultivation density.
Current fermented feed is all using anaerobism or aerobic type, and solid-fermented technique, moisture content is in 35-60 part
Between, it is sealed, applies after fermentation.
One development trend of aquaculture exactly gradually adopts particulate material to replace pervious powder, to improve cultivation effect
Benefit reduces environmental pollution.But raiser adds additive for microbe feedstuff or fermentation material in particulate material, it is very inconvenient, and
And fermentation material particle is all that moisture granulation is dried by high temperature, high temperature can make bacterium loss of activity, also not meet the thermophilic soft diet of animal
Property.
Summary of the invention
The purpose of the present invention is to provide a kind of high-survival rate fermented shrimp material, this seed shrimp material is solved because extruding is pelletized,
The problem of micro ecological product can not be utilized, the present invention pass through the breeding that every component compounding inhibits pathogen in shrimp enteron aisle, repair
Multiple shrimp enteron aisle, enhances the anti-ability of shrimp, reduces antibiotic usage, improves the unique effects such as survival rate.
In order to reach above-mentioned technical effect, the present invention the following technical schemes are provided:
It includes following material composition that height, which survives fermented shrimp material score by weight: 25-33 parts of fish meal, 30 parts -46 parts of dregs of beans, wheat
3-7 parts of bran, 4-9 parts of cornstarch, 3-8 parts of flour, black soldier flies starch 6-15 parts, 0.2-1 parts of maltodextrin, sodium alginate 0.1-
0.5 part, sub- liquid 10-22 parts of activated spawn.
Preferably, the height survives fermented shrimp material in parts by weight by following material composition: 30 parts of fish meal, 33 parts of dregs of beans,
5 parts of wheat bran, 5 parts of cornstarch, 5 parts of flour, black soldier flies starch 6.5 parts, 0.5 part of maltodextrin, 0.5 part of sodium alginate, activation bacterium
15 parts of seed liquor, 0.5 part of diammonium hydrogen citrate.
Preferably, the sub- liquid of the activated spawn includes enterococcus faecalis, bacillus subtilis, saccharomyces cerevisiae, lichens gemma bar
Bacterium, lactobacillus reuteri composition.
Preferably, enterococcus faecalis in the sub- liquid of the activated spawn, bacillus subtilis, saccharomyces cerevisiae, bacillus licheniformis,
Lactobacillus reuteri volume ratio: 3-4:1-2:2-3:1-2:3-4.
A kind of a kind of preparation method preparing high-survival rate fermented shrimp material the following steps are included:
(1) activation bacterium is transferred in sterile water respectively, forms the sub- liquid of activated spawn, concentration is 1.0 × 109Cfu/ml~
5.0×109cfu/ml；
(2) seed culture fluid that step (1) obtains is transferred to respectively in the fermentor containing fermentation medium, culture is arrived
18-24 hours, concentration was 5.0 × 108Cfu/ml~8.0 × 109cfu/ml；
(3) fish meal, dregs of beans, wheat bran, cornstarch, flour, black soldier flies slurry, maltodextrin are all crossed into 0.18-0.30mm
Sieve.
(4) liquid spawn that obtains step (2) and in mixed liquor, the diammonium hydrogen citrate of 0.1 part -0.5 part of addition,
It is inoculated into step (3) the fermented shrimp material feedstuff, inoculum concentration is 10-22 parts；
(5) the uniformly mixed feedstuff that step (4) obtains is sub-packed in 20-35 kilograms of special be vented certainly and unidirectionally sends out
In ferment bag, it is placed in ferment at constant temperature in 25-32 DEG C of environment, fermentation time is -11 days 7 days, and surface of material is covered with white bacterium point, and companion
There are strong aroma, sour taste, carries out next stage immediately；
(6) the fermented shrimp feed powder material that step (5) obtains is unpacked at sterile, in the spinning extrusion granulator to have sterilized in advance
It pelletizes on machine, is sub-packed in the 1-5 kilograms of unidirectional fermentation bag of special exhaust certainly after granulation at sterile；It is placed in 25-32 DEG C of environment
Secondary ferment at constant temperature, fermentation time are -5 days 3 days.
Black soldier flies slurry: black soldier flies are septic Stratiomyidae insect, can feeding fowl and animal excrement and house refuse, production is high
The animal protein feed of value, having breeding, biomass is big rapidly, and feeding habits are extensive, sorption enhanced rate is high, is easy management, raising
At low cost, the features such as animal palatability is good, black soldier flies starch protein and the content of fatty acid is very high, its thick protein contains
The Plant protein feeds such as amount and semen viciae fabae, sunflower seeds are very close, but the fatty content that it is wherein contained is higher than them,
Amino acid content is similar to fish meal, but is higher than common bean powder.
Enterococcus faecalis: enterococcus faecalis (Enterococcus faecalis) is Gram-positive, hydrogen peroxide feminine gender ball
Bacterium is one of flora in humans and animals enteron aisle, can generate natural antibiotics, be conducive to body health；It can also produce simultaneously
The antibacterial substances such as endophytic bacteria element inhibit the growth of the pathogens such as Escherichia coli and salmonella, improve intestinal microenvironment；It can also press down
The breeding of urease-producing bacterium and spoilage organisms in enteron aisle processed reduces enteron aisle urease and endotoxic content, make in blood ammonia and
Endotoxic content decline.
Bacillus subtilis: bacillus subtilis is to harmful micro- life such as vibrios, Escherichia coli and baculoviral in aquatic products
Object has very strong inhibiting effect, effectively prevents aquatic livestock enteritis, the diseases such as gill rot.Secretion is a large amount ofChitinaseFunction, it is several
Fourth matter enzyme can decompose disease fungusCell wallAnd inhibit fungal disease, it decomposes in aquaculture pondPoisonous and harmful substance, purified water
Matter；Residual bait, excrement, organic matter etc. in decomposing pool have the short grained effect of rubbish in very strong cleaning water；Bacillus subtilis
Shrimp disease can be reduced, prawn yield can be greatly improved, to increase economic efficiency, biological environmental production stimulates aquatic products
The development of animal immune organ enhances immunity of organisms；Reduce shrimp disease, hence it is evident that prawn yield is improved, to improve
Economic benefit purifies water, pollution-free, noresidue.
Saccharomyces cerevisiae: also known asSaccharomyces cerevisiaeOr budding yeast.Saccharomyces cerevisiae be with the widest primary yeast of human relation,
Not only due to traditionally it for make bread andSteamed bunEqual food and wine brewing are used as true in modern molecular and cell biology
Kernel normal form biology, functions as the model organism of protokaryonEscherichia coli。
Bacillus licheniformis: the adjustable intestinal flora of bacillus licheniformis, the effect of antagonism pathogenic bacteria.The bacterium after oral
Into people's enteron aisle, there is antibacterial action to staphylococcus and saccharomycete, and to Bifidobacterium Bifidum, Bacillus acidi lactici, bacteroid, streptococcus fecalis
Growth then have facilitation.This product has the characteristics that rapid-action, high curative effect is the one kind for treating enteric infection or flora imbalance
Safe and reliable probiotics.Suitable for enterobacteriaceae caused by acute and chronic diarrhea caused by bacterium and mould and a variety of causes
The prevention and treatment of group's imbalance.
Lactobacillus reuteri: lactobacillus reuteri is reported at present almost to may be present in all vertebrates and lactation
Bacillus acidi lactici in animal intestinal tract.It is to have to improve allergic constitution, pre- hypo-allergenic recurrent exerbation, and adjust the benefit of function of intestinal canal
Raw bacterium.
The utility model has the advantages that
1, high-survival rate fermented shrimp material provided by the invention adds enterococcus faecalis, bacillus subtilis, wine brewing in shrimp material
The bacterium such as yeast, bacillus licheniformis, lactobacillus reuteri are by making shrimp material raw material predigestion, while fermentating metabolism generates largely
Antibacterial substance, lactic acid, digestive ferment etc. can improve the utilization rate of feed, inhibit the breeding of pathogen in shrimp enteron aisle, repair shrimp intestines
Road enhances the anti-ability of shrimp, reduces antibiotic usage, improves the unique effects such as survival rate.
2, the preparation method of high-survival rate fermented shrimp material provided by the invention reaches low temperature using spinning extrusion granulator machine and makes
The purpose of grain, the special proportion of proportion, inoculum concentration and its shrimp material in conjunction with various bacterium in the sub- liquid of activated spawn solve routine
Damage of the granulator pelletization high temperature to microorganism and bioactive substance, at the same solve common fermentation feed powder it is more, waste
Greatly, to severe water pollution, thus the problem of largely effecting on shrimp survival rate, enterococcus faecalis, bacillus subtilis, saccharomyces cerevisiae,
The peculiar ratio of bacillus licheniformis, lactobacillus reuteri, histamine content in the feed that can effectively degrade reduce burden of liver, mention
High shrimp turns the motility rate deposited the liver phase, adds the flour, maltodextrin, sodium alginate of different proportion, efficiently solves fermented shrimp material system
Water resistance after grain and aqueous inadequate problem is sunk, in the microorganisms energy fast degradation water such as bacillus licheniformis, bacillus subtilis
The organic matters such as excrement, residual bait, the harmful substances such as ammonia nitrogen, nitrite in degrading cultivation water.
Specific embodiment
The present invention is further elaborated with the following Examples:
Embodiment 1
A kind of preparation method of high viability fermented shrimp material, method includes the following steps:
(1): by the enterococcus faecalis of activation, bacillus subtilis, saccharomyces cerevisiae, bacillus licheniformis (Bacillus
Licheniformis), lactobacillus reuteri slant strains are transferred in sterile water respectively, formed seed liquor, concentration be 1.0 ×
109cfu/mlcfu/ml；
(2): the seed culture fluid that step (1) obtains is transferred to respectively with 3 parts of inoculum concentration containing fermentation medium
In fermentor, control fermentation temperature, stirring rate, ventilatory capacity, tank pressure, the parameters such as oxyty, when culture was by 18 hours, concentration
It is 5.0 × 108cfu/ml；(37 ± 1 DEG C, stirring rate 0rpm, ventilatory capacity 0V/ of enterococcus faecalis, lactobacillus reuteri fermentation temperature
Vmin, tank press 0.04-0.05Mpa, 5-10 parts of oxyty；Bacillus subtilis, the lichen bacillus ferments temperature 37 ± 1
DEG C, stirring rate 220rpm, ventilatory capacity 10V/Vmin, tank press 0.04-0.05Mpa, 60-80 parts of oxyty；Saccharomyces cerevisiae
28 ± 1 DEG C of fermentation temperature, stirring rate 160rpm, ventilatory capacity 5V/Vmin, tank presses 0.04-0.05Mpa, oxyty 50-70
Part)
(3) mass fraction, the fermented shrimp feedstuff mainly consist of the following components: 25 parts of fish meal, dregs of beans 30 are pressed
Part, 3 parts of wheat bran, 4 parts of cornstarch, 3 parts of flour, black soldier flies starch 6 parts, 0.2 part of maltodextrin, 0.1 part of sodium alginate.The original
Material all crosses 0.18-0.30mm sieve.
(4) liquid spawn for obtaining step (2), according to enterococcus faecalis, bacillus subtilis, saccharomyces cerevisiae, lichens bud
Spore bacillus, lactobacillus reuteri volume ratio 3:1:2:1:3 ratio are uniformly mixed, and in mixed liquor, the citric acid of 0.1 part of addition
Hydrogen diammonium is inoculated into step (3) the fermented shrimp material feedstuff, and inoculum concentration is 10 parts；
(5) it is unidirectional to be sub-packed in 20-35 kilograms of special exhaust certainly for the uniformly mixed feedstuff for obtaining step (4)
In fermentation bag, it is placed in ferment at constant temperature in 25-32 DEG C of environment, everyday for 7, surface of material is covered with white bacterium point, and companion to fermentation time
There are strong aroma, sour taste, carries out next stage immediately；
(6) the fermented shrimp feed powder material that step (5) obtains is unpacked at sterile, in the spinning extrusion granulator to have sterilized in advance
It pelletizes on machine, is sub-packed in the 1-5 kilograms of unidirectional fermentation bag of special exhaust certainly after granulation at sterile；It is placed in 25-32 DEG C of environment
Secondary ferment at constant temperature, fermentation time are 3 days.
Embodiment 2
A kind of preparation method of high viability fermented shrimp material, method includes the following steps:
(1): by enterococcus faecalis (Enterococcus faecalis), the bacillus subtilis (Bacillus of activation
Subtilis), saccharomyces cerevisiae (Saccharomyces cerevisiae), bacillus licheniformis (Bacillus
Licheniformis), lactobacillus reuteri (Lactobacillus reuteri) slant strains are transferred in sterile water respectively, shape
At seed liquor, concentration is 5.0 × 109cfu/ml；
(2): the seed culture fluid that step (1) obtains is transferred to respectively with 8 parts of inoculum concentration containing fermentation medium
In fermentor, the parameters such as control fermentation temperature, stirring rate, ventilatory capacity, tank pressure, oxyty, culture arrives 18-24 hours, dense
Degree is 8.0 × 109cfu/ml；(37 ± 1 DEG C, stirring rate 0rpm of enterococcus faecalis, lactobacillus reuteri fermentation temperature, ventilatory capacity
0V/Vmin, tank press 0.04-0.05Mpa, 5-10 parts of oxyty；Bacillus subtilis, the lichen bacillus ferments temperature 37
± 1 DEG C, stirring rate 220rpm, ventilatory capacity 10V/Vmin, tank press 0.04-0.05Mpa, 60-80 parts of oxyty；Wine brewing ferment
28 ± 1 DEG C, stirring rate 160rpm, ventilatory capacity 5V/Vmin of female fermentation temperature, tank presses 0.04-0.05Mpa, oxyty 50-
70 parts)
(3): pressing mass fraction, the fermented shrimp feedstuff mainly consist of the following components: 33 parts of fish meal, dregs of beans 46
Part, 7 parts of wheat bran, 9 parts of cornstarch, 8 parts of flour, black soldier flies starch 15 parts, 1 part of maltodextrin, 0.5 part of sodium alginate.The original
Material all crosses 0.18-0.30mm sieve.
(4) liquid spawn for obtaining step (2), according to enterococcus faecalis, bacillus subtilis, saccharomyces cerevisiae, lichens bud
Spore bacillus, lactobacillus reuteri volume ratio: 4:2:3:2::4: ratio is uniformly mixed, and in mixed liquor, the lemon of 0.5 part of addition
Lemon acid hydrogen diammonium is inoculated into step (3) the fermented shrimp material feedstuff, and inoculum concentration is 22 parts；
(5) it is unidirectional to be sub-packed in 20-35 kilograms of special exhaust certainly for the uniformly mixed feedstuff for obtaining step (4)
In fermentation bag, it being placed in ferment at constant temperature in 25-32 DEG C of environment, fermentation time is 11 days, and surface of material is covered with white bacterium point, and with
Strong aroma, sour taste, carry out next stage immediately；
(6) the fermented shrimp feed powder material that step (5) obtains is unpacked at sterile, in the spinning extrusion granulator to have sterilized in advance
It pelletizes on machine, is sub-packed in the 1-5 kilograms of unidirectional fermentation bag of special exhaust certainly after granulation at sterile；It is placed in 25-32 DEG C of environment
Secondary ferment at constant temperature, fermentation time are 5 days.
Embodiment 3
A kind of preparation method of high viability fermented shrimp material, method includes the following steps:
(1): by enterococcus faecalis (Enterococcus faecalis), the bacillus subtilis (Bacillus of activation
Subtilis), saccharomyces cerevisiae (Saccharomyces cerevisiae), bacillus licheniformis (Bacillus
Licheniformis), lactobacillus reuteri (Lactobacillus reuteri) slant strains are transferred in sterile water respectively, shape
At seed liquor, concentration is 5.0 × 109cfu/ml；
(2): the seed culture fluid that step (1) obtains is transferred to respectively with 8 parts of inoculum concentration containing fermentation medium
In fermentor, the parameters such as control fermentation temperature, stirring rate, ventilatory capacity, tank pressure, oxyty, culture arrives 18-24 hours, dense
Degree is 8.0 × 109cfu/ml；(37 ± 1 DEG C, stirring rate 0rpm of enterococcus faecalis, lactobacillus reuteri fermentation temperature, ventilatory capacity
0V/Vmin, tank press 0.04-0.05Mpa, 5-10 parts of oxyty；Bacillus subtilis, the lichen bacillus ferments temperature 37
± 1 DEG C, stirring rate 220rpm, ventilatory capacity 10V/Vmin, tank press 0.04-0.05Mpa, 60-80 parts of oxyty；Wine brewing ferment
28 ± 1 DEG C, stirring rate 160rpm, ventilatory capacity 5V/Vmin of female fermentation temperature, tank presses 0.04-0.05Mpa, oxyty 50-
70 parts)
(3) by mass percentage, the fermented shrimp feedstuff mainly consist of the following components: 30 parts of fish meal, dregs of beans
33 parts, 5 parts of wheat bran, 5 parts of cornstarch, 5 parts of flour, black soldier flies slurry 6.5 parts, 0.5 part of maltodextrin, 0.5 part of sodium alginate, institute
It states raw material and all crosses 0.18-0.30mm sieve.
(4) liquid spawn for obtaining step (2), according to enterococcus faecalis, bacillus subtilis, saccharomyces cerevisiae, lichens bud
Spore bacillus, lactobacillus reuteri volume ratio 4:2:3:2::4: ratio is uniformly mixed, and in mixed liquor, the lemon of 0.5 part of addition
Sour hydrogen diammonium is inoculated into step (3) the fermented shrimp material feedstuff, and inoculum concentration is 15 parts；
(5) it is unidirectional to be sub-packed in 20-35 kilograms of special exhaust certainly for the uniformly mixed feedstuff for obtaining step (4)
In fermentation bag, it being placed in ferment at constant temperature in 25-32 DEG C of environment, fermentation time is 11 days, and surface of material is covered with white bacterium point, and with
Strong aroma, sour taste, carry out next stage immediately；
(6) the fermented shrimp feed powder material that step (5) obtains is unpacked at sterile, in the spinning extrusion granulator to have sterilized in advance
It pelletizes on machine, is sub-packed in the 1-5 kilograms of unidirectional fermentation bag of special exhaust certainly after granulation at sterile；It is placed in 25-32 DEG C of environment
Secondary ferment at constant temperature, fermentation time are 5 days.
Comparative example 1-9 fermented shrimp material proportion see the table below 1
Table 1
The preparation method of comparative example 1-9 and other preparation conditions are same as Example 3.
The difference of comparative example 10 and embodiment 3 be only that the sub- liquid of activated spawn without enterococcus faecalis, other preparation methods with
Preparation condition is same as Example 3.
The difference of comparative example 11 and embodiment 3 is only that the sub- liquid of activated spawn without bacillus subtilis, other preparation sides
Method is same as Example 3 with preparation condition.
The difference of comparative example 12 and embodiment 3 be only that the sub- liquid of activated spawn without saccharomyces cerevisiae, other preparation methods with
Preparation condition is same as Example 3.
The difference of comparative example 13 and embodiment 3 is only that the sub- liquid of activated spawn without bacillus licheniformis, other preparation sides
Method is same as Example 3 with preparation condition.
The difference of comparative example 14 and embodiment 3 is only that the sub- liquid of activated spawn without lactobacillus reuteri, other preparation sides
Method is same as Example 3 with preparation condition.
Comparative example 15 is certain commercially available shrimp material.
Shrimp seedling is divided into 18 parts, it is commercially available using the embodiment 1-3 and comparative example 1-14 shrimp material prepared and comparative example 15 respectively
Shrimp material feed is raised under identical environment, and survival rate and rate of body weight gain see the table below 2.
| Survival rate (%) | Rate of body weight gain (%) | Specific growth rate | |
| Embodiment 1 | 94.23±0.05 | 196.23±0.05 | 2.64±0.05 |
| Embodiment 2 | 96.76±0.06 | 198.34±0.06 | 2.76±0.07 |
| Embodiment 3 | 97.34±0.03 | 199.23±0.05 | 2.56±0.05 |
| Comparative example 1 | 87.76±0.05 | 163.35±0.05 | 2.07±0.05 |
| Comparative example 2 | 83.56±0.05 | 173.54±0.05 | 1.97±0.05 |
| Comparative example 3 | 84.77±0.05 | 153.67±0.05 | 1.87±0.05 |
| Comparative example 4 | 84.96±0.05 | 133.97±0.05 | 1.85±0.05 |
| Comparative example 5 | 82.33±0.05 | 163.17±0.05 | 1.47±0.05 |
| Comparative example 6 | 85.76±0.05 | 153.22±0.05 | 1.67±0.05 |
| Comparative example 7 | 86.97±0.05 | 163.44±0.05 | 1.69±0.05 |
| Comparative example 8 | 82.43±0.05 | 153.44±0.05 | 1.56±0.05 |
| Comparative example 10 | 83.76±0.04 | 165.23±0.04 | 1.97±0.05 |
| Comparative example 11 | 85.12±0.05 | 143.34±0.05 | 1.97±0.05 |
| Comparative example 12 | 86.76±0.06 | 176.43±0.06 | 1.56±0.05 |
| Comparative example 13 | 80.12±0.06 | 157.23±0.04 | 1.87±0.05 |
| Comparative example 14 | 78.24±0.07 | 145.87±0.04 | 1.97±0.05 |
| Comparative example 15 | 78.23±0.05 | 127.86±0.06 | 1.86 ± 0,05 |
It is compared by comparative example 1-9 and comparative example 3 as it can be seen that black soldier flies slurry, maltodextrin and activated spawn in inventive formulation
Sub- liquid collective effect, which reaches, guarantees shrimp seedling survival rate and rate of body weight gain, compares visible activated spawn by comparative example 10-14 and embodiment 3
Enterococcus faecalis, bacillus subtilis, saccharomyces cerevisiae, bacillus licheniformis, lactobacillus reuteri are synergistic in sub- liquid.
This hair can be understood and used the above description of the embodiments is intended to facilitate those skilled in the art
It is bright.Person skilled in the art obviously easily can make various modifications to these embodiments, and described herein
General Principle is applied in other embodiments without having to go through creative labor.Therefore, the present invention is not limited to the above embodiments,
Those skilled in the art's announcement according to the present invention, improvement and modification made without departing from the scope of the present invention all should be in this hairs
Within bright protection scope.
Claims (5)
1. a kind of high-survival rate fermented shrimp material, which is characterized in that it includes following object that the height, which survives fermented shrimp material score by weight,
Matter composition: 25-33 parts of fish meal, 30 parts -46 parts of dregs of beans, 3-7 parts of wheat bran, 4-9 parts of cornstarch, 3-8 parts of flour, black soldier flies starch 6-
15 parts, 0.2-1 parts of maltodextrin, 0.1-0.5 parts of sodium alginate, sub- liquid 10-22 parts of activated spawn.
2. a kind of high-survival rate fermented shrimp material as described in claim 1, which is characterized in that the height survives fermented shrimp material by weight
Score is measured by following material composition: 30 parts of fish meal, 33 parts of dregs of beans, 5 parts of wheat bran, 5 parts of cornstarch, 5 parts of flour, black soldier flies slurry
6.5 parts, 0.5 part of maltodextrin, 0.5 part of sodium alginate, 15 parts, 0.5 part of diammonium hydrogen citrate of the sub- liquid of activated spawn.
3. a kind of high-survival rate fermented shrimp material as described in claim 1, which is characterized in that contain in the sub- liquid of activated spawn
Enterococcus faecalis, bacillus subtilis, saccharomyces cerevisiae, bacillus licheniformis, lactobacillus reuteri.
4. a kind of high-survival rate fermented shrimp material as claimed in claim 3, which is characterized in that excrement intestines in the sub- liquid of activated spawn
Coccus, bacillus subtilis, saccharomyces cerevisiae, bacillus licheniformis, lactobacillus reuteri volume ratio: 3-4:1-2:2-3:1-2:3-
4。
5. it is a kind of prepare a kind of preparation method of high-survival rate fermented shrimp material described in claim 1-4 the following steps are included:
(1) seed culture fluid that step (1) obtains is transferred to the hair containing fermentation medium with 3 parts -8 parts of inoculum concentration respectively
In fermentation tank, the sub- liquid of activated spawn is formed, concentration is 1.0 × 109Cfu/ml~5.0 × 109cfu/ml；
(2) seed culture fluid that step (1) obtains is transferred to respectively in the fermentor containing fermentation medium, 18- is arrived in culture
24 hours, concentration was 5.0 × 108Cfu/ml~8.0 × 109cfu/ml；
(3) fish meal, dregs of beans, wheat bran, cornstarch, flour, black soldier flies slurry, maltodextrin are all crossed into 0.18-0.30mm sieve.
(4) liquid spawn that obtains step (2) and in mixed liquor, the diammonium hydrogen citrate of 0.1 part -0.5 part of addition, inoculation
Into step (3) the fermented shrimp material feedstuff, inoculum concentration is 10-22 parts；
(5) the uniformly mixed feedstuff that step (4) obtains is sub-packed in 20-35 kilograms of the special unidirectional fermentation bag of exhaust certainly
In, it is placed in ferment at constant temperature in 25-32 DEG C of environment, fermentation time is -11 days 7 days, and surface of material is covered with white bacterium point, and with dense
Strongly fragrant aroma, sour taste, carry out next stage immediately；
(6) the fermented shrimp feed powder material that step (5) obtains is unpacked at sterile, in the spinning extrusion granulator machine to have sterilized in advance
Granulation is sub-packed in the 1-5 kilograms of unidirectional fermentation bag of special exhaust certainly after granulation at sterile；It is placed in secondary in 25-32 DEG C of environment
Ferment at constant temperature, fermentation time are -5 days 3 days.
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| CN111066945A (en) * | 2019-12-19 | 2020-04-28 | 广东省微生物研究所（广东省微生物分析检测中心） | Pelletization-free prawn fermented feed |
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| CN114886056A (en) * | 2022-06-02 | 2022-08-12 | 湖南艾布鲁环保科技股份有限公司 | Heisui river horsefly compound shrimp and crab feed and shrimp and crab ecological breeding method |
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