CN107535699A - A kind of preparation method rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe - Google Patents
A kind of preparation method rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe Download PDFInfo
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Abstract
The application is related to a kind of preparation method rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe.After gathering rumen fluid, strain liquid directly or after in vitro culture is made.After smart crude feed product is crushed, sized mixing, fermentation tank is pumped into；Heating up and be passed through CO2After deoxygenation, micro-element nutrition buffer solution and strain liquid are added, zymotic fluid is made after strictly anaerobic fermentation, then solid content wet basis and liquid nutrient solution is made in separation of solid and liquid.After liquid nutrient solution directly concentrates, liquid nutritional concentrated liquor is made；Enzymatic hydrolysis and fermentation after zymotic fluid solid content wet basis mixes with feedstuff, clean water, composite bacteria liquid, complex enzyme, glycolysis solid content wet basis is made.After glycolysis solid content wet basis is mixed with nutrition concentrated liquor, it is made rich in volatile fatty acid calcium fermented feed.Rich in nutrients such as volatile fatty acid calcium, mycoprotein, active small peptide, oligosaccharide, probiotics, having stimulates lactation, promotes digestion and improves feed intake and other effects feed prepared by the application.
Description
Technical field
The application belongs to feed, enzyme preparation and microbial technology field, and in particular to one kind is rich in volatile fatty acid calcium
The preparation method of cud source fermentative feedstuff of microbe.
Background technology
For many years, rumen microbial fermentation and its nutrition are always the research hot topic topic of ruminant animal nutrition.Cud
It is first stomach of the ruminant close to esophagus, volume there are about 70-120 litres, can be regarded as one and supplies anaerobic microorganism
The active fermentation tank of the vaccinization of breeding.In a sense, people raise ruminant and are really feeding its knurl
Microorganism in stomach.Research is found：Rumen microorganism mainly has bacterium, fungi and rumen metabolism.Bacterium includes:Fiber degradation
Bacterium, hemicellulose degradation bacteria, starch degrading bacterium, protein degradation bacterium, fat acid decomposition bacterium, acid-utilising bacterium, Lactic Acid Producing and methane phase
Bacterium etc., quantity are 108-1010/mL (rumen fluid).Rumen Fungi is anaerobic fungi, and cell membrane all contains chitin, produces trip
Dynamic spore, and nutritious body stage, there are as many as 16 kinds of 6 category, about 103-105/mL (rumen fluid) of quantity.Rumen metabolism
It is divided into two classes：One kind is cud infusorian；One kind is cud flagellate.Infusorian about hundreds of, the size of polypide between
Between 20-200 μm, quantity is 105-106/mL (rumen fluid), and gross weight accounts for the 20% of rumen content.And cud flagellum
Worm has 5 kinds, and the size of polypide is seldom relative to cud infusorian quantity in rumen fluid between 4-15 μm.
Rumen microorganism can produce various digestive ferments, the daily ration that host feeds be fermented, most of bacteriums can send out
One or more structural polysaccharides and depot polysaccharide in ferment feed, energy source is provided for its growth.And some bacteriums are then
Unfermentable carbohydrate, but it can utilize the simple product after carbohydrate hydrolysis or the major end products of carbohydate metabolism, such as lactic acid, formic acid
Or hydrogen etc. obtains energy.In rumen microorganism bacterial species, a part is the flora of decomposing protein and amino acid, and it ferments
Caused ammonia, amino acid and small peptide, and then synthesize rumen mycoprotein matter.
The final product of rumen microbial fermentation carbohydrate be volatile fatty acid (abbreviation VFA), B family vitamin,
VK and microbial bacteria body protein (MCP).Volatile fatty acid is the organic aliphatic acid that carbochain is 1-6, including acetic acid, propionic acid, different
Butyric acid, valeric acid, isovaleric acid, n-butyric acie etc..The common feature of volatile fatty acid is that have stronger volatility, therefore claims volatility
Aliphatic acid.Volatile fatty acid is the important intermediate of cud anaerobic fermentation digestion process.In ruminant body, to animal
Body is metabolized most importantly acetic acid, propionic acid and butyric acid.Wherein, second, third, butyric acid account for the 95% of lumen fermentation VFA total outputs,
And yield of acetic acid is maximum, the 70-75% of VFA total outputs is accounted for.Especially significantly：Recent research also found volatile fat
Lactation, growth, immune and breeding of the sour calcium (VFA-Ca) to ruminant all have extremely important effect.
Therefore, ruminant tumor gastric is a big fermentation tank full of microorganisms such as bacterium, infusorian and fungies.Certain meaning
Said in justice, raising ruminant is exactly to raise the microorganism in its cud, and the viable counts of rumen microorganism, species and fermentation
Product determines whether cud is healthy to a certain extent.In turn, the health of cud not only influences the growth of ruminant, milk production
Amount and milk quality, have an effect on the fertility of milk cow.
The enzymolysis feed that ferments is using microorganism, complex enzyme as biological feed fermentation agent strain, feedstuff is converted into micro-
Biological tropina, bioactive micro peptide amino acid, microbial activity probiotics, complex enzyme formulation are integrated biofermentation feeding
Material.Fermentation enzymolysis feed is not only suitable for the livestock animals such as pig, ox, sheep, is applied to the poultry animals such as chicken, duck, goose again, and be applicable
In various animals such as fishes and shrimps aquatic products, yellow meal worm insects.Fermentation enzymolysis Feed Energy makes feed removing toxic substances detoxification, greatly improves the peace of feed
Quan Xing, and light fragrance can be produced in the enzymolysis process that ferments, so as to add the palatability of animal.Ferment enzymolysis process
In can also generate a variety of organic acids, vitamin, biology enzyme, amino acid and other a variety of UGFs, substantially increase hair
The trophic level and digestive utilization ratio of ferment enzymolysis feed.Meanwhile the enzymolysis feed that ferments also improves the resistance of animal, reduces feed
Cost.But for existing fermented feed, remain in problems with：First, it is compound using separating both at home and abroad
Strain or industrialization strain carry out fermented feed, not yet find to be used directly to fermented feed using rumen microorganism or its nutrient solution；
2nd, both at home and abroad is to regulate and control rumen microbial fermentation pH using buffer solution, not yet find using calcium hydroxide or calcium carbonate come
Regulate and control rumen microbial fermentation pH, and the thus report of self-assembling formation volatile fat acid calcium salt or research；3rd, domestic and international cud
Liquid microbial liquid fermented feed, which exists, dries the shortcomings of difficult and drying cost is high, not yet finds rumen fluid microbial fermentation
Feed separation of solid and liquid, and carry out report or the research of liquid concentration and the fermentation of solid content secondary enzymolysis.
The content of the invention
The application be intended to overcome the above-mentioned regulation and control of rumen fluid microbial liquid fermented feed buffer solution both at home and abroad pH, dry it is difficult,
The shortcomings of drying cost is high, there is provided one kind is rich in volatile fatty acid calcium, rumen mycoprotein matter, active small peptide, oligosaccharide, benefit
The nutrients such as raw bacterium, having stimulates lactation, promotes digestion and improves the cud source fermentative feedstuff of microbe of feed intake and other effects.
The technical solution adopted by the present invention is as follows：
A kind of preparation method rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe, comprises the following steps：
1., the preparation of rumen microorganism strain liquid：
The rumen fluid of healthy ruminant is gathered, rumen microorganism strain liquid is made；
2., the preparation of rumen microbial fermentation liquid：
After crushing by concentrated feed and crude feed product, add water to size mixing, fermentation tank is pumped into；Heating up and be passed through CO2Exclude to raise
In slurry liquid after oxygen, add micro-element nutrition buffer solution and rumen microorganism strain liquid, continuous stream be hydrogenated with calcium oxide or
After strictly anaerobic ferments some hours under conditions of calcium carbonate fine powder, rumen microbial fermentation liquid is made；
3., the preparation of rumen microbial fermentation liquid solid content wet basis and liquid nutrient solution：
Equipped through separation of solid and liquid, it is wet that rumen microbial fermentation liquid separation of solid and liquid is made into rumen microbial fermentation liquid solid content
Base and liquid nutrient solution；
4., the preparation of liquid nutritional concentrated liquor：
Through steam concentrator, after liquid nutrient solution is directly concentrated, liquid nutritional concentrated liquor is prepared；
5., the preparation of glycolysis solid content wet basis：
Enzymatic hydrolysis and fermentation after rumen microbial fermentation liquid solid content wet basis is mixed with composite bacteria liquid, complex enzyme, ferment is made
Solve solid content wet basis；
Or rumen microbial fermentation liquid solid content wet basis is mixed with feedstuff, clean water, composite bacteria liquid, complex enzyme
Enzymatic hydrolysis and fermentation afterwards, glycolysis solid content wet basis is made；
6., the preparation rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe：
After rumen microbial fermentation liquid solid content wet basis is mixed with nutrition concentrated liquor, directly production is rich in volatility fat
Dry after fat acid calcium cud source fermentative feedstuff of microbe wet basis, or mixing or be mixed after being respectively dried rich in volatile fat
Sour calcium cud source fermentative feedstuff of microbe butt；
Or after glycolysis solid content wet basis is mixed with nutrition concentrated liquor, directly production is rich in volatile fatty acid calcium cud
Dry or be mixed after being respectively dried rich in volatile fatty acid calcium cud source after source fermentative feedstuff of microbe wet basis, or mixing
Fermentative feedstuff of microbe butt.
Preferably, the concentrated feed is the one or more of feedstuff in Minister Agriculture of China's feedstuff catalogue, mainly
For dregs of beans, cotton dregs, rapeseed dregs, peanut meal, fish meal, blood meal, plasma proteins, hyperglobulinemia, milk powder, lactose, whey powder, wheat
Gluten, corn protein powder, rice protein powder, DDGS, soybean, pea, red bean, corn, paddy, crack rice, wheat, wheat bran, rice
Chaff, flour, oat, barley, sweet potato, soybean protein isolate, FSPC, fruit and vegetable, root tuber stem tuber, sugar molasse sugar
One or more in slurry and food processing industry byproduct, or the one or more in the dilated product of above-mentioned concentrated feed；
The roughage is the one or more of feedstuff in Minister Agriculture of China's feedstuff catalogue, predominantly crops
One or more in stalk, pasture powder, the leaf leaf of bamboo and Chinese herbal medicine, or above-mentioned roughage.
Preferably, the concentrated feed is the one or more of feedstuff in Minister Agriculture of China's feedstuff catalogue, mainly
For dregs of beans, cotton dregs, rapeseed dregs, peanut meal, corn protein powder, DDGS, soybean, pea, corn, paddy, wheat, wheat bran, rice bran,
One or more in oat, barley, sugar molasse syrup and food processing industry byproduct；
The roughage is the one or more of feedstuff in Minister Agriculture of China's feedstuff catalogue, predominantly crops
Stalk and pasture powder.
Preferably, the preparation condition of step 2. middle rumen microbial fermentation liquid is as follows：
(1), fermentation temperature：10~60 DEG C；
(2), ferment pH：3.0~9.0；
(3), fermented type：Continuously it is passed through CO20.1-60 hours, form strictly anaerobic fermentation condition；
(4), rumen microorganism strain inoculum concentration：0.1~50wt.%
(5), fermentation time：1-360 hours.
Preferably, the preparation condition of step 2. middle rumen microbial fermentation liquid is as follows：
(1), fermentation temperature：38~40 DEG C；
(2), ferment pH：5.0~7.0；
(3), fermented type：Continuously it is passed through CO20.5-1 hours, form strictly anaerobic fermentation condition；
(4), rumen microorganism strain inoculum concentration：5~10wt.%
(5), fermentation time：72-96 hours.
Preferably, the preparation condition of step 4. middle liquid nutritional concentrated liquor is as follows：
(1), thickening temperature：30~100 DEG C；
(2), concentrate solid content：40~80wt.%.
Preferably, the preparation condition of step 4. middle liquid nutritional concentrated liquor is as follows：
(1), thickening temperature：55~65 DEG C；
(2), concentrate solid content：50~55wt.%.
Preferably, the preparation condition of step 5. middle glycolysis solid content wet basis is as follows：
(1), rumen microbial fermentation liquid solid content wet basis and feedstuff, clean water, composite bacteria liquid, the weight of complex enzyme
Amount is than being 100：0~500：0~1000：0.5~50：0.0001~10；
(2), the yeast seeds liquid in composite bacteria liquid：Bacillus subtilis strain liquid：The viable bacteria of lactic acid bacteria culturers liquid
Quantity ratio is 100：0~200：0~100；
(3), the 1,4 beta-glucanase in complex enzyme, beta-glucosidase：Zytase：Mannase：The work of pectase
Property ratio be 100：0~1000：0~500：0~300：0~200；
(4), enzymatic hydrolysis and fermentation temperature：10~60 DEG C, enzymatic hydrolysis and fermentation starting pH：3~9, first anaerase after aerobic enzymatic hydrolysis and fermentation
Hydrolysis and fermentation 12-240 hours.
Preferably, the preparation condition of step 5. middle glycolysis slag dregs of rice wet basis is as follows：
(1), rumen microbial fermentation liquid solid content wet basis and feedstuff, clean water, composite bacteria liquid, the weight of complex enzyme
Amount is than being 100：50~100：0~10：5~10：0.05~0.1；
(2), the yeast seeds liquid in composite bacteria liquid：Bacillus subtilis strain liquid：The viable bacteria of lactic acid bacteria culturers liquid
Quantity ratio is 100：30~50：10~20；
(3), the 1,4 beta-glucanase in complex enzyme, beta-glucosidase：Zytase：Mannase：The work of pectase
Property ratio be 100：40~60：30~50：30~50：10~20；
(4), enzymatic hydrolysis and fermentation temperature：30~40 DEG C, enzymatic hydrolysis and fermentation starting pH：6~7, first anaerase after aerobic enzymatic hydrolysis and fermentation
Hydrolysis and fermentation 48-60 hours.
It should be noted that：Micro-element nutrition buffer solution is according to the method introduced in the paper delivered before Cheng Maoji etc.
Prepare.Specifically paper is：Cheng Maoji, Lu Dexun, Wang Hongrong, Wang Gang, Zhao Xiuying, rumen microorganism external degradation separate sources peptide
Speed research,《Agricultural University Of Nanjing's journal》- 2003,26 (1)：50-55.
The beneficial effects of the present invention are：Prepared by the present invention raises rich in volatile fatty acid calcium cud source microbial fermentation
Material, rich in nutrients such as volatile fatty acid calcium, rumen mycoprotein matter, active small peptide, oligosaccharide, probiotics, secretes with stimulation
Breast, promote digestion and improve feed intake and other effects.
Embodiment
Embodiment 1
A kind of preparation method rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe, comprises the following steps：
1., the preparation of rumen microorganism strain liquid：
The rumen fluid 20kg (195L) of 5 healthy cows is gathered, rumen microorganism strain liquid 200kg is made；
2., the preparation of rumen microbial fermentation liquid：
After 50kg dregs of beans, 150kg corns, the crushing of 400kg wheat straws plus 1400kg water are sized mixing, fermentation tank is pumped into；It is passed through steaming
Vapour is warming up to 39 DEG C and continues to be passed through CO in 20 minutes2Exclude in feed slurries after oxygen, add 20kg micro-element nutritions buffering
Liquid and 200kg rumen microorganism strain liquids, strictly anaerobic ferments under conditions of continuous stream is hydrogenated with calcium oxide or calcium carbonate fine powder
After 72 hours, 2200kg rumen microbial fermentation liquid is made.
3., the preparation of rumen microbial fermentation liquid solid content wet basis and liquid nutrient solution：
Equipped through separation of solid and liquid, 1050kg rumen microorganisms hair is made in 2200kg rumen microbial fermentation liquid separation of solid and liquid
Zymotic fluid solid content wet basis and 1150kg liquid nutrient solutions.
4., the preparation of liquid nutritional concentrated liquor：
Through steam concentrator, after 1150kg liquid nutrient solutions are directly concentrated, the concentration of 250kg liquid nutrient solutions is prepared
Liquid.
5., the preparation of glycolysis solid content wet basis：
1050kg rumen microbial fermentations liquid solid content wet basis and 250kg wheat brans, 80kg composite bacterias liquid, 0.5kg is multiple
Enzymatic hydrolysis and fermentation 60 hours, is made 1350kg glycolysis solid content wet basis after synthase mixing.
6., the preparation rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe：
After 1350kg rumen microbial fermentations liquid solid content wet basis is mixed with 250kg nutrition concentrated liquors, mixed after drying
Conjunction is made 815kg and is rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe butt.
Wherein, the yeast seeds liquid in composite bacteria liquid：Bacillus subtilis strain liquid：The viable bacteria of lactic acid bacteria culturers liquid
Quantity ratio is 100:30:30；
1,4 beta-glucanase, beta-glucosidase in complex enzyme：Zytase：Mannase：The active ratio of pectase
For 100:50:50:50:50；
Embodiment 2
A kind of preparation method rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe, comprises the following steps：
1., the preparation of rumen microorganism strain liquid：
The rumen fluid 5kg (4.9L) of 5 healthy goats is gathered, rumen microorganism strain liquid 50kg is made；
2., the preparation of rumen microbial fermentation liquid：
After 10kg dregs of beans, 50kg corns, the crushing of 100kg wheat straws plus 340kg water are sized mixing, fermentation tank is pumped into；It is passed through steam
It is warming up to 39 DEG C and continues to be passed through CO in 10 minutes2Exclude in feed slurries after oxygen, add 3kg micro-element nutritions buffer solution and
50kg rumen microorganism strain liquids, strictly anaerobic ferments 96 hours under conditions of continuous stream is hydrogenated with calcium oxide or calcium carbonate fine powder
Afterwards, 550kg rumen microbial fermentation liquid is made.
3., the preparation of rumen microbial fermentation liquid solid content wet basis and liquid nutrient solution：
Equipped through separation of solid and liquid, 220kg rumen microbial fermentations are made in 550kg rumen microbial fermentation liquid separation of solid and liquid
Liquid solid content wet basis and 330kg liquid nutrient solutions.
4., the preparation of liquid nutritional concentrated liquor：
Through steam concentrator, after 330kg liquid nutrient solutions are directly concentrated, the concentration of 90kg liquid nutrient solutions is prepared
Liquid.
5., the preparation of glycolysis solid content wet basis：
Enzyme after 220kg rumen microbial fermentation liquid solid content wet basis 10kg composite bacterias liquid, 0.05kg complex enzymes are mixed
Hydrolysis and fermentation 71 hours, 222kg glycolysis solid content wet basis is made.
6., the preparation rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe：
After 222kg rumen microbial fermentations liquid solid content wet basis is mixed with 90kg nutrition concentrated liquors, mixed after drying
150kg is made and is rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe butt.
Wherein, the yeast seeds liquid in composite bacteria liquid：Bacillus subtilis strain liquid：The viable bacteria of lactic acid bacteria culturers liquid
Quantity ratio is 100:50:30；
1,4 beta-glucanase, beta-glucosidase in complex enzyme：Zytase：Mannase：The active ratio of pectase
For 100:60:40:30:30；
Embodiment 3
1., the preparation of rumen microorganism strain liquid：
The rumen fluid 30kg (195L) of 5 healthy beef cattles is gathered, rumen microorganism strain liquid 300kg is made；
2., the preparation of rumen microbial fermentation liquid：
After 100kg cotton dregs, 100kg corns, 100kg barleys, the crushing of 700kg wheat straws plus 2500kg water are sized mixing, hair is pumped into
Fermentation tank；Steam is passed through to be warming up to 39 DEG C and continue to be passed through CO in 20 minutes2Exclude in feed slurries after oxygen, to add the micro members of 50kg
Plain nutrition buffer solution and 300kg rumen microorganism strain liquid is tight under conditions of continuous stream is hydrogenated with calcium oxide or calcium carbonate fine powder
After lattice anaerobic fermentation 72 hours, 4300kg rumen microbial fermentation liquid is made.
3., the preparation of rumen microbial fermentation liquid solid content wet basis and liquid nutrient solution：
Equipped through separation of solid and liquid, 2100kg rumen microorganisms hair is made in 4300kg rumen microbial fermentation liquid separation of solid and liquid
Zymotic fluid solid content wet basis and 2200kg liquid nutrient solutions.
4., the preparation of liquid nutritional concentrated liquor：
Through steam concentrator, after 2200kg liquid nutrient solutions are directly concentrated, the concentration of 420kg liquid nutrient solutions is prepared
Liquid.
5., the preparation of glycolysis solid content wet basis：
By 2100kg rumen microbial fermentations liquid solid content wet basis and 200kg wheat brans, 200 cotton dregs, 200kg composite bacterias
Enzymatic hydrolysis and fermentation 72 hours, is made 2650kg glycolysis solid content wet basis after liquid, the mixing of 0.8kg complex enzymes.
6., the preparation rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe：
After 2650kg rumen microbial fermentations liquid solid content wet basis is mixed with 420kg nutrition concentrated liquors, mixed after drying
Conjunction is made 1175kg and is rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe butt.
Wherein, the yeast seeds liquid in composite bacteria liquid：Bacillus subtilis strain liquid：The viable bacteria of lactic acid bacteria culturers liquid
Quantity ratio is 100:40:20；
1,4 beta-glucanase, beta-glucosidase in complex enzyme：Zytase：Mannase：The active ratio of pectase
For 100:50:50:20:20；
Embodiment 4
1., the preparation of rumen microorganism strain liquid：
The rumen fluid 20kg (195L) of 5 healthy cows is gathered, rumen microorganism strain liquid 200kg is made；
2., the preparation of rumen microbial fermentation liquid：
100kg cotton dregs, 200kg corns, 200kg barleys, 200kg wheat straws are crushed, adds, after 1600kg water is sized mixing, is pumped into
Fermentation tank；Steam is passed through to be warming up to 39 DEG C and continue to be passed through CO in 10 minutes2Exclude that in feed slurries after oxygen, it is micro to add 30kg
Element nutrition buffer solution and 200kg rumen microorganism strain liquids, under conditions of continuous stream is hydrogenated with calcium oxide or calcium carbonate fine powder
After strictly anaerobic ferments 72 hours, 2530kg rumen microbial fermentation liquid is made.
3., the preparation of rumen microbial fermentation liquid solid content wet basis and liquid nutrient solution：
Equipped through separation of solid and liquid, 650kg rumen microorganisms hair is made in 2530kg rumen microbial fermentation liquid separation of solid and liquid
Zymotic fluid solid content wet basis and 1880kg liquid nutrient solutions.
4., the preparation of liquid nutritional concentrated liquor：
Through steam concentrator, after 1880kg liquid nutrient solutions are directly concentrated, the concentration of 580kg liquid nutrient solutions is prepared
Liquid.
5., the preparation of glycolysis solid content wet basis：
By 650kg rumen microbial fermentations liquid solid content wet basis and 100 cotton dregs, 80kg composite bacterias liquid, 0.3kg complex enzymes
Enzymatic hydrolysis and fermentation 72 hours after mixing, 810kg glycolysis solid content wet basis is made.
6., the preparation rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe：
After 810kg rumen microbial fermentations liquid solid content wet basis is mixed with 580kg nutrition concentrated liquors, mixed after drying
785kg is made and is rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe butt.
Wherein, the yeast seeds liquid in composite bacteria liquid：Bacillus subtilis strain liquid：The viable bacteria of lactic acid bacteria culturers liquid
Quantity ratio is 100:50:20；
1,4 beta-glucanase, beta-glucosidase in complex enzyme：Zytase：Mannase：The active ratio of pectase
For 100:50:40:30:20；
Embodiment 5
1., the preparation of rumen microorganism strain liquid：
The rumen fluid 15kg (195L) of 2 healthy cows and 3 healthy cows is gathered, rumen microorganism strain liquid is made
150kg；
2., the preparation of rumen microbial fermentation liquid：
100kg cotton dregs, 200kg corns and 200kg wheat brans are crushed, adds, after 1200kg water is sized mixing, is pumped into fermentation tank；It is logical
Enter steam to be warming up to 39 DEG C and continue to be passed through CO in 15 minutes2Exclude in feed slurries after oxygen, to add 20kg micro-element nutritions
Buffer solution and 150kg rumen microorganism strain liquids, the strictly anaerobic under conditions of continuous stream is hydrogenated with calcium oxide or calcium carbonate fine powder
After fermentation 72 hours, 1870kg rumen microbial fermentation liquid is made.
3., the preparation of rumen microbial fermentation liquid solid content wet basis and liquid nutrient solution：
Equipped through separation of solid and liquid, 550kg rumen microorganisms hair is made in 1870kg rumen microbial fermentation liquid separation of solid and liquid
Zymotic fluid solid content wet basis and 1320kg liquid nutrient solutions.
4., the preparation of liquid nutritional concentrated liquor：
Through steam concentrator, after 1320kg liquid nutrient solutions are directly concentrated, the concentration of 510kg liquid nutrient solutions is prepared
Liquid.
5., the preparation of glycolysis solid content wet basis：
By 550kg rumen microbial fermentations liquid solid content wet basis and 200 cotton dregs, 60kg composite bacterias liquid, 0.3kg complex enzymes
Enzymatic hydrolysis and fermentation 72 hours after mixing, 785kg glycolysis solid content wet basis is made.
6., the preparation rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe：
After 785kg rumen microbial fermentations liquid solid content wet basis is mixed with 510kg nutrition concentrated liquors, mixed after drying
685kg is made and is rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe butt.
Wherein, the yeast seeds liquid in composite bacteria liquid：Bacillus subtilis strain liquid：The viable bacteria of lactic acid bacteria culturers liquid
Quantity ratio is 100:60:20；
1,4 beta-glucanase, beta-glucosidase in complex enzyme：Zytase：Mannase：The active ratio of pectase
For 100:60:50:40:30；
What the first, prepared by embodiment 1 and embodiment 5 is rich in main in the fermentative feedstuff of microbe of volatile fatty acid calcium cud source
Activity substance content, it see the table below 1,2.
Prepared by table 1, embodiment 1 is rich in nutrition content in the product of microorganism fermented forage of VFA-Ca cuds source
Prepared by table 2, embodiment 5 is rich in nutrition content in the product of microorganism fermented forage of VFA-Ca cuds source
2nd, the feeding cow effect rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe prepared by embodiment 1.
Volatile fatty acid calcium cud source fermentative feedstuff of microbe is rich in lactating cow for prepared by discussion embodiment 1
Feeding effect, choose date of parturition difference and be no more than 3 days, genetic background identical He Sitan 2-3 tires lactating cow 60, by body
Principle similar in weight, parity is randomly divided into 3 groups, and every group of 5 repetitions are each to repeat 2 cow heads.Control group daily ration does not add use
Fermented feed.Common commercially available fermented feed group daily ration addition uses common commercially available fermented feed 60kg/ tons.Test group addition addition
Using rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe 60kg/ tons.Each group feed protein and energy are close,
For the TMR daily rations of standard.90 days experimental periods.Result of the test such as table 3 below：
Shadow rich in VFA-Ca cuds source fermentative feedstuff of microbe to lactating cow production performance prepared by table 3, embodiment 1
Ring
| Project | Control group | Common commercially available fermented feed group | Cud source fermentative feedstuff of microbe group |
| Feed intake (kg) | 22.35 | 23.02 | 26.77 |
| The output of milk (kg) | 38.70 | 39.07 | 43.24 |
| 3.5% correction milk (kg) | 38.51 | 38.73 | 42.98 |
| Butterfat percnetage/% | 3.22 | 3.25 | 3.67 |
| Feed efficiency | 1.81 | 1.78 | 1.69 |
Common commercially available fermented feed 60kg/ tons are added in daily ration and rich in volatile fatty acid calcium cud source microbial fermentation
After feed 60kg/ tons, the feed intake of lactating cow improves 3.00% and 19.78% than control group respectively, and the output of milk compares respectively
0.96% and 11.73% are improved according to group, 3.5% correction milk improves 5.71% and 11.61%, butterfat percnetage than control group respectively
0.93% and 13.98% is improved than control group respectively, feed efficiency (milk feed ratio) reduces 1.66% He than control group respectively
6.63%.As a result show：Will to the feeding effect of lactating cow rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe
Significantly better than control group.
Compared with adding common commercially available fermented feed 60kg/ tons, addition is rich in volatile fatty acid calcium cud source microorganism
After fermented feed 60kg/ tons, the feed intake of lactating cow improves 14.01%, and the output of milk improves 9.64% than control group respectively,
3.5% correction milk improves 9.89% than control group respectively, and butterfat percnetage improves 11.44% than control group respectively, feed efficiency (milk
Material ratio) reduce 5.32% than control group respectively.As a result show：Rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe
Common commercially available fermented feed will be significantly better than to the feeding effect of lactating cow.
Above content is only that affiliated those skilled in the art are to being retouched to the application example and explanation
The specific embodiment stated is made various modifications or supplement or substituted using similar mode, without departing from the design of invention
Or surmount scope defined in the claims, the protection domain of the application all should be belonged to.
Claims (9)
- A kind of 1. preparation method rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe, it is characterised in that including with Lower step：1., the preparation of rumen microorganism strain liquid：The rumen fluid of healthy ruminant is gathered, rumen microorganism strain liquid is made；2., the preparation of rumen microbial fermentation liquid：After crushing by concentrated feed and crude feed product, add water to size mixing, fermentation tank is pumped into；Heating up and be passed through CO2Exclude feed slurries After middle oxygen, micro-element nutrition buffer solution and rumen microorganism strain liquid are added, calcium oxide or calcium carbonate are hydrogenated with continuous stream After strictly anaerobic ferments some hours under conditions of fine powder, rumen microbial fermentation liquid is made；3., the preparation of rumen microbial fermentation liquid solid content wet basis and liquid nutrient solution：Equipped through separation of solid and liquid, by rumen microbial fermentation liquid separation of solid and liquid be made rumen microbial fermentation liquid solid content wet basis and Liquid nutrient solution；4., the preparation of liquid nutritional concentrated liquor：Through steam concentrator, after liquid nutrient solution is directly concentrated, liquid nutritional concentrated liquor is prepared；5., the preparation of glycolysis solid content wet basis：Enzymatic hydrolysis and fermentation after rumen microbial fermentation liquid solid content wet basis is mixed with composite bacteria liquid, complex enzyme, obtained glycolysis are consolidated Shape thing wet basis；Or enzyme after rumen microbial fermentation liquid solid content wet basis is mixed with feedstuff, clean water, composite bacteria liquid, complex enzyme Hydrolysis and fermentation, glycolysis solid content wet basis is made；6., the preparation rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe：After rumen microbial fermentation liquid solid content wet basis is mixed with nutrition concentrated liquor, directly production is rich in volatile fatty acid Dry or be mixed after being respectively dried rich in volatile fatty acid calcium after calcium cud source fermentative feedstuff of microbe wet basis, or mixing Cud source fermentative feedstuff of microbe butt；Or after glycolysis solid content wet basis is mixed with nutrition concentrated liquor, directly production is micro- rich in volatile fatty acid calcium cud source Dry or be mixed after being respectively dried rich in the micro- life in volatile fatty acid calcium cud source after biological fermentation feed wet basis, or mixing Thing fermented feed butt.
- A kind of 2. preparation side rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe according to claim 1 Method, it is characterised in thatThe concentrated feed be Minister Agriculture of China's feedstuff catalogue in feedstuff one or more, predominantly dregs of beans, cotton dregs, Rapeseed dregs, peanut meal, fish meal, blood meal, plasma proteins, hyperglobulinemia, milk powder, lactose, whey powder, wheat gluten flour, corn Albumen powder, rice protein powder, DDGS, soybean, pea, red bean, corn, paddy, crack rice, wheat, wheat bran, rice bran, flour, swallow Wheat, barley, sweet potato, soybean protein isolate, FSPC, fruit and vegetable, root tuber stem tuber, sugar molasse syrup and food add One or more in one or more in industry byproduct, or the dilated product of above-mentioned concentrated feed；The roughage is the one or more of feedstuff in Minister Agriculture of China's feedstuff catalogue, predominantly crops straw One or more in stalk, pasture powder, the leaf leaf of bamboo and Chinese herbal medicine, or above-mentioned roughage.
- A kind of 3. preparation rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe according to claim 1 or 2 Method, it is characterised in thatThe concentrated feed be Minister Agriculture of China's feedstuff catalogue in feedstuff one or more, predominantly dregs of beans, cotton dregs, Rapeseed dregs, peanut meal, corn protein powder, DDGS, soybean, pea, corn, paddy, wheat, wheat bran, rice bran, oat, barley, sugar One or more in class molasses syrup and food processing industry byproduct；The roughage is the one or more of feedstuff in Minister Agriculture of China's feedstuff catalogue, predominantly agricultural crop straw And pasture powder.
- A kind of 4. preparation side rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe according to claim 1 Method, it is characterised in that the preparation condition of step 2. middle rumen microbial fermentation liquid is as follows：(1), fermentation temperature：10~60 DEG C；(2), ferment pH：3.0~9.0；(3), fermented type：Continuously it is passed through CO20.1-60 hours, form strictly anaerobic fermentation condition；(4), rumen microorganism strain inoculum concentration：0.1~50wt.%(5), fermentation time：1-360 hours.
- A kind of 5. preparation rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe according to claim 1 or 4 Method, it is characterised in that the preparation condition of step 2. middle rumen microbial fermentation liquid is as follows：(1), fermentation temperature：38~40 DEG C；(2), ferment pH：5.0~7.0；(3), fermented type：Continuously it is passed through CO20.5-1 hours, form strictly anaerobic fermentation condition；(4), rumen microorganism strain inoculum concentration：5~10wt.%(5), fermentation time：72-96 hours.
- A kind of 6. preparation side rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe according to claim 1 Method, it is characterised in that the preparation condition of step 4. middle liquid nutritional concentrated liquor is as follows：(1), thickening temperature：30~100 DEG C；(2), concentrate solid content：40~80wt.%.
- A kind of 7. preparation rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe according to claim 1 or 6 Method, it is characterised in that the preparation condition of step 4. middle liquid nutritional concentrated liquor is as follows：(1), thickening temperature：55~65 DEG C；(2), concentrate solid content：50~55wt.%.
- A kind of 8. preparation side rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe according to claim 1 Method, it is characterised in that the preparation condition of step 5. middle glycolysis solid content wet basis is as follows：(1), rumen microbial fermentation liquid solid content wet basis and feedstuff, clean water, composite bacteria liquid, the weight ratio of complex enzyme For 100：0~500：0~1000：0.5~50：0.0001~10；(2), the yeast seeds liquid in composite bacteria liquid：Bacillus subtilis strain liquid：The number of viable of lactic acid bacteria culturers liquid Than for 100：0~200：0~100；(3), the 1,4 beta-glucanase in complex enzyme, beta-glucosidase：Zytase：Mannase：The active ratio of pectase For 100：0~1000：0~500：0~300：0~200；(4), enzymatic hydrolysis and fermentation temperature：10~60 DEG C, enzymatic hydrolysis and fermentation starting pH：3~9, first anaerobism enzymolysis is sent out after aerobic enzymatic hydrolysis and fermentation Ferment 12-240 hours.
- A kind of 9. preparation rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe according to claim 1 or 8 Method, it is characterised in that the preparation condition of step 5. middle glycolysis slag dregs of rice wet basis is as follows：(1), rumen microbial fermentation liquid solid content wet basis and feedstuff, clean water, composite bacteria liquid, the weight ratio of complex enzyme For 100：50~100：0~10：5~10：0.05~0.1；(2), the yeast seeds liquid in composite bacteria liquid：Bacillus subtilis strain liquid：The number of viable of lactic acid bacteria culturers liquid Than for 100：30~50：10~20；(3), the 1,4 beta-glucanase in complex enzyme, beta-glucosidase：Zytase：Mannase：The active ratio of pectase For 100：40~60：30~50：30~50：10~20；(4), enzymatic hydrolysis and fermentation temperature：30~40 DEG C, enzymatic hydrolysis and fermentation starting pH：6~7, first anaerobism enzymolysis is sent out after aerobic enzymatic hydrolysis and fermentation Ferment 48-60 hours.
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