CN100387148C - Method for preparing cow rumen regulator - Google Patents
Method for preparing cow rumen regulator Download PDFInfo
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- CN100387148C CN100387148C CNB2004100591370A CN200410059137A CN100387148C CN 100387148 C CN100387148 C CN 100387148C CN B2004100591370 A CNB2004100591370 A CN B2004100591370A CN 200410059137 A CN200410059137 A CN 200410059137A CN 100387148 C CN100387148 C CN 100387148C
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Abstract
The present invention discloses a preparation method for a cow rumen regulator, which is mainly and technically characterized in that beer yeast and candida mycoderma are inoculated in an enriched medium of wort for primary enrichment culture and secondary enrichment culture medium; viable bacteria are inoculated in a liquid culture medium and a new substrate and are uniformly mixed for fermentation twice; a fermentation product is baked and ground after the fermentation so as to obtain a finished product. The preparation method has the advantages of scientific strain compatibility, no mutual repulsion in the process of culture and reasonable proportion of fermentation substrates and particle size. The product conforms to the desired nutrition and the conditions of the fermentation of each strain, and the fermentation potentiality of each strain is fully exerted. The product prepared by the method can promote and maintain the balance of microbiota in a rumen, stabilize the pH value of the rumen, relieve the acidosis of a milk cow caused by highly concentrated feed, reduce the concentration of ammonia in the rumen, promote the use of nitrogen, improve a microecological environment and increase the coarse feed utilization rate of the milk cow.
Description
Technical field
The present invention relates to a kind of preparation method of cow rumen regulation and control agent, refer in particular to saccharomycete and adopt bio-fermentation process, inoculation medium is carried out making after the degree of depth fermentation preparation method of cow rumen regulation and control agent.
Background technology
1. support the cud of the key of good milk cow, only possess the milk cow of good function of rumen, could bring into play its prouctiveness to greatest extent conditioned milk cow.By active material is provided, thereby activate growth and the metabolism of rumen microorganism in cud, can reach the purpose of regulation and control function of rumen.
2. cow rumen regulation and control agent comprises the mixture of yeast culture, it can provide the cow rumen microorganism needed nutrition substrate, stimulate the active of microbial bacterial and quicken the speed of its synthetic nutrient, make the microorganism in the cud provide more nutrient and energy for milk cow.
3. yeast culture is different from active dry yeasr.It is to be difficult to survival that probioticses such as active dry yeasr enter in the cud, and they can be subjected to the attack of complicated microorganism in the cud and the inhibition of the interior environment of cud, does not also have enough time often to breed and produce metabolite just to be killed.Therefore can not obtain whole beneficiating ingredients of being contained in the yeast culture by the active dry yeasr of feeding, so the preparation method who develops a kind of cow rumen regulation and control agent is very important.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of preparation method of cow rumen regulation and control agent.Prepare this cow rumen regulation and control agent and select the high-quality saccharomycete for use, and adopt advanced bio-fermentation process, inoculation medium is carried out degree of depth fermentation after, extracellular metabolic product, cellular content and sex change culture medium mixed and make.The bacterial classification that this preparation method uses increases bacterium cultivation acquisition by the saccharomycete of a few strain high-qualitys through two-stage.
Technical problem of the present invention is solved by following scheme:
The preparation method of cow rumen regulation and control agent, it is characterized in that: with brewer's yeast on the test tube slant, Candida is inoculated in the triangular flask that the brewer's wort enriched medium is housed in the ratio of every ring 40～80ml respectively, bottle stopper beyond the Great Wall, and wrap with sealing film, in temperature is 27-32 ℃, vibration was carried out one-level in 36-72 hour and is increased the bacterium cultivation under the 100-150 rev/min of condition, to increase the saccharomyces cerevisiae liquid that bacterium is cultivated through one-level respectively again, it is that 4-10 doubly is equipped with in the triangular flask of brewer's wort enriched medium that candidiasis liquid is inoculated into volume, in temperature is 27-32 ℃, vibration was carried out secondary in 36-72 hour and is increased the bacterium cultivation under the 100-150 rev/min of condition, to increase the saccharomyces cerevisiae liquid that bacterium is cultivated through secondary again, be inoculated into behind the candidiasis liquid mixing and be equipped with in the bottle that volume is a 10-20 times of fluid nutrient medium, fermented 48-72 hour 30-40 ℃ of condition next time, adding volume in the zymotic fluid that ferments is that 60-140 new substrate mixing doubly carried out secondary fermentation 36-72 hour, fermentation temperature is controlled at 30-40 ℃, adopt drying machine drying after the fermentation ends, the inlet temperature of dryer is not higher than 140 ℃, medium temperature is not higher than 50 ℃, outlet temperature is not higher than 80 ℃, and the material after the oven dry is pulverized, packing is finished product.
Technical problem of the present invention also can be solved by following scheme:
Described brewer's wort enriched medium adds the distilled water of 40-60 times of volume by brewer's wort, and pol transfers to 4-10 ripple woods, and PH transfers to 5.5-6.5,100-125 ℃ down sterilization made in 15-30 minute.
Described fluid nutrient medium is mixed by the weight ratio of corn flour 10%-15%, wheat bran 10%-15%, wheat flour 5%-8%, diastatic malt 5%-10%, molasses 20%-30%, running water 20%-40%, adjusting PH is 4-6, sterilizes down at temperature 100-125 ℃ and makes in 15-30 minute.
Described new substrate is made by 1: 0.33～0.76 weight ratio mixing with water after evenly being mixed by the weight ratio of dregs of beans 1%-3%, albumen dregs of rice 8%-10%, certain herbaceous plants with big flowers dregs of rice 10%-20%, alcohol dregs of rice 10%-15%, corn 10%-15%, sesame seed meal 3%-5%, rice chaff dregs of rice 5%-10%, peanut chaff 3%-6%, rice bran 5%-8%, wheat bran 15%-22%, sulfuric acid amine 6%-8% again.
The granularity that described material is pulverized is 75% to cross 20 mesh sieves by 80 mesh sieves, 95% by 40 mesh sieves, 100%.
Advantage of the present invention is:
1. promote and safeguard microbiota balance in the cud, stablize ruminal pH value, alleviate the milk cow acid poisoning that high fine fodder causes, the concentration of ammonia promotes its utilization to nitrogen in the reduction cud, improves its micro-ecological environment.
2. the breeding of cellulose-decomposing bacteria and lactic acid bacteria in the stimulation cud improves the utilization rate of milk cow to roughage, improves the palatability of feed, increases the feed intake of dry, improves the performance of giving milk.
3. increase the anti-stress ability of milk cow, can resist the negative effect that heat stress brings to milk cow especially.This product contains bioactivators such as oligosaccharides, small-molecular peptides, protein, amino acid, beta glucan, mannose, alcohols, ester class.
4. the bacterial classification compatibility is scientific and reasonable, does not have to repel mutually in incubation to produce; Fermentation substrate proportioning and reasonable particle size meet the required nutrition and the condition of fermentation of every kind of bacterial classification, can give full play to the fermentation potentiality of every kind of bacterial classification;
Description of drawings
This accompanying drawing is cow rumen regulation and control agent preparation technology figure.
The specific embodiment
With brewer's yeast, Candida respectively from the test tube slant the picking five rings be inoculated into the triangular flask (capacity is 500ml) that 300ml brewer's wort enriched medium is housed, be to carry out 48 hours one-levels under 28 ℃ of conditions to increase bacterium and cultivate in temperature; To increase the saccharomyces cerevisiae liquid that bacterium is cultivated through one-level, each 60ml of candidiasis liquid is inoculated into respectively and is equipped with in the triangular flask that volume is the brewer's wort enriched medium of 600ml (capacity is 1000ml), in temperature is 30 ℃, vibration is carried out 48 hours secondarys and is increased the bacterium cultivation under 120 rev/mins of conditions, to increase the saccharomyces cerevisiae liquid 40ml that bacterium is cultivated through secondary again, candidiasis liquid 80ml is inoculated into and is equipped with in the triangular flask that volume is 1600 gram fluid nutrient mediums (the triangular flask capacity is 2000ml), fermented 60 hours 35 ℃ of conditions next time, adding volume in the zymotic fluid that ferments is that the 172000 new substrate mixings that restrain carried out secondary fermentation 60 hours, fermentation temperature is controlled at 35 ℃, dry after the fermentation ends, the inlet temperature of dryer is not higher than 140 ℃, medium temperature is not higher than 50 ℃, outlet temperature is not higher than 80 ℃, material after the oven dry is pulverized, and the granularity of pulverizing is 75% by 80 mesh sieves, 95% by 40 mesh sieves, 100% crosses 20 mesh sieves.Packing is finished product again.
Described secondary brewer's wort enriched medium adds the distilled water of 882ml by the 18ml brewer's wort, and pol transfers to 8 ripple woodss, and PH transfers to 6.0,120 ℃ down sterilization made in 30 minutes.
Described 1600 gram fluid nutrient mediums are mixed by corn flour 160 gram, wheat bran 240 grams, wheat flour 112 grams, diastatic malt 128 grams, molasses 320 grams and running water 640 grams, and regulating PH is 5,120 ℃ of temperature down sterilization made in 30 minutes.
Described new substrate 172000 grams by dregs of beans 1720 grams, the albumen dregs of rice 13760 grams, the certain herbaceous plants with big flowers dregs of rice 25800 grams, the alcohol dregs of rice 17200 grams, corn 25800 grams, sesame seed meal 8600 grams, the rice chaff dregs of rice 12040 grams, peanut chaff 8600 grams, rice bran 10320 grams, wheat bran 36120 grams, sulfuric acid amine 12040 gram mixings after, add 86000 gram water mixings again and make.
Claims (2)
1. the preparation method of cow rumen regulation and control agent, it is characterized in that: with brewer's yeast on the test tube slant, Candida is inoculated in the bottle that the brewer's wort enriched medium is housed in the ratio of every ring 40～80ml respectively, bottle stopper beyond the Great Wall, and wrap with sealing film, in temperature is 27-32 ℃, vibration was carried out one-level in 36-72 hour and is increased the bacterium cultivation under the 100-150 rev/min of condition, to increase the saccharomyces cerevisiae liquid that bacterium is cultivated through one-level respectively again, it is that 4-10 doubly is equipped with in the bottle of brewer's wort enriched medium that candidiasis liquid is inoculated into volume, in temperature is 27-32 ℃, vibration was carried out secondary in 36-72 hour and is increased the bacterium cultivation under the 100-150 rev/min of condition, to increase the saccharomyces cerevisiae liquid that bacterium is cultivated through secondary again, be inoculated into behind the candidiasis liquid mixing and be equipped with in the bottle that volume is a 10-20 times of fluid nutrient medium, fermented 48-72 hour 30-40 ℃ of condition next time, adding volume in the zymotic fluid that ferments is that 60-140 new substrate mixing doubly carried out secondary fermentation 36-72 hour, fermentation temperature is controlled at 30-40 ℃, adopt drying machine drying after the fermentation ends, the inlet temperature of dryer is not higher than 140 ℃, medium temperature is not higher than 50 ℃, outlet temperature is not higher than 80 ℃, material after the oven dry is pulverized, packing is finished product, described brewer's wort enriched medium is added the distilled water of 40-60 times of volume by brewer's wort, pol transfers to 4-10 ripple woods, PH transfers to 5.5-6.5, sterilization was made in 15-30 minute under 100-125 ℃, described fluid nutrient medium is by corn flour 10%-15%, wheat bran 10%-15%, wheat flour 5%-8%, diastatic malt 5%-10%, molasses 20%-30%, the weight ratio of running water 20%-40% mixes, adjusting PH is 4-6, sterilization was made in 15-30 minute under temperature 100-125 ℃, described new substrate is by dregs of beans 1%-3%, albumen dregs of rice 8%-10%, certain herbaceous plants with big flowers dregs of rice 10%-20%, alcohol dregs of rice 10%-15%, corn 10%-15%, sesame seed meal 3%-5%, rice chaff dregs of rice 5%-10%, peanut chaff 3%-6%, rice bran 5%-8%, wheat bran 15%-22%, the weight ratio of sulfuric acid amine 6%-8% is made by 1: 0.33～0.76 weight ratio mixing with water after evenly mixing again.
2. preparation method according to claim 1 is characterized in that: the granularity that described material is pulverized is 75% to cross 20 mesh sieves by 80 mesh sieves, 95% by 40 mesh sieves, 100%.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2004100591370A CN100387148C (en) | 2004-08-13 | 2004-08-13 | Method for preparing cow rumen regulator |
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| CNB2004100591370A CN100387148C (en) | 2004-08-13 | 2004-08-13 | Method for preparing cow rumen regulator |
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| CN1732797A CN1732797A (en) | 2006-02-15 |
| CN100387148C true CN100387148C (en) | 2008-05-14 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101390572B (en) * | 2008-10-22 | 2011-07-06 | 南京农业大学 | Efficient cow rumen regulation and control agent |
| CN102499331B (en) * | 2011-12-28 | 2014-01-15 | 内蒙古伊利实业集团股份有限公司 | Dairy cow rumen regulating agent and preparation method thereof |
| CN103719537A (en) * | 2013-12-18 | 2014-04-16 | 四川宜宾全福饲料有限公司 | Nonreactive biological fermented feed and preparation method thereof |
| CN106508810A (en) * | 2016-12-30 | 2017-03-22 | 新疆西部牧业股份有限公司 | Angus beef finished cattle feeding and management method |
| CN114532452B (en) * | 2022-04-26 | 2022-07-15 | 北京挑战农业科技有限公司 | Method for preparing ruminal fermented feed by using defective fruits and application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1259299A (en) * | 1999-11-24 | 2000-07-12 | 吉林大学 | Dehusk and detoxin of cotton-seed cake to produce protein feed |
| CN1310949A (en) * | 2001-01-17 | 2001-09-05 | 中国科学院微生物研究所 | Method for producing straw protein fodder by using microbiological straw fermentation |
| CN1435113A (en) * | 2003-03-06 | 2003-08-13 | 天津科技大学 | Process for producing stalk protein feed by multi-culture united anaerobic fermentation |
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- 2004-08-13 CN CNB2004100591370A patent/CN100387148C/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1259299A (en) * | 1999-11-24 | 2000-07-12 | 吉林大学 | Dehusk and detoxin of cotton-seed cake to produce protein feed |
| CN1310949A (en) * | 2001-01-17 | 2001-09-05 | 中国科学院微生物研究所 | Method for producing straw protein fodder by using microbiological straw fermentation |
| CN1435113A (en) * | 2003-03-06 | 2003-08-13 | 天津科技大学 | Process for producing stalk protein feed by multi-culture united anaerobic fermentation |
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