CN104232550B - A kind of lactic acid bacteria enriched medium and preparation method thereof - Google Patents
A kind of lactic acid bacteria enriched medium and preparation method thereof Download PDFInfo
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- CN104232550B CN104232550B CN201410512085.1A CN201410512085A CN104232550B CN 104232550 B CN104232550 B CN 104232550B CN 201410512085 A CN201410512085 A CN 201410512085A CN 104232550 B CN104232550 B CN 104232550B
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Abstract
A kind of lactic acid bacteria enriched medium, lactic acid bacteria enriched medium described in preparation 1L, consist of the following composition: the corn starch of 10 30g and brown sugar, the bean milk of 30 50g and egg yolk, the corn juice of 120 180mL, the medicated beer of 120 180mL and the Radix Dauci Sativae juice of 70 130mL, remaining complements to 1L with distilled water.The raw materials used low price of inventive formulation, makes culture medium prices, decreases enterprise's production cost.
Description
Technical field
The present invention relates to a kind of culture medium, be specifically related to a kind of lactic acid bacteria enriched medium.
Background technology
The preparation etc. of lactic acid bacteria separation and Culture and enzymology, lactobacillus leaven is required for good enriched medium, lactic acid bacteria is made to be bred in a large number, so that lactic acid bacteria has enough viable bacteria remainings through spray drying, freezing, lyophilization etc. after processing.The formula of culture medium is mainly made up of carbon source, nitrogen source, somatomedin etc., and existing culture medium prescription is mostly the raw material of industry, needs chemical industry shop to buy, and price comparison is expensive.
Summary of the invention
In order to solve the problems referred to above, the present invention provides raw material in a kind of formula to be easy to buy, and uses simple, lactic acid bacteria enriched medium of low cost and preparation method thereof.
The purpose of the present invention is achieved through the following technical solutions:
A kind of lactic acid bacteria enriched medium, lactic acid bacteria enriched medium described in preparation 1L, consist of the following composition: the corn starch of 10-30g and brown sugar, the bean milk of 30-50g and egg yolk, the corn juice of 120-180mL, the medicated beer of 120-180mL and the Radix Dauci Sativae juice of 70-130mL, remaining complements to 1L with distilled water；Described corn starch and brown sugar are carbon source, and its ratio is corn starch: brown sugar=1:2；Described bean milk and egg yolk are nitrogen source, and its ratio is bean milk: egg yolk=1:2；Described Radix Dauci Sativae juice is to be shredded by fresh carrot, after boiling with distilled water, and the juice obtained by filtered through gauze.
Described corn juice, medicated beer and Radix Dauci Sativae juice are somatomedin.
The manufacture method of a kind of lactic acid bacteria enriched medium, comprises the following steps:
(1) pretreatment of raw material: the manufacture method of bean milk is to take 50g Semen Glycines soy bean milk making machine to wear into bean milk, with distilled water constant volume to 500g；Egg yolk directly isolated from raw egg；The manufacture method of corn juice is that seed shelled by fresh Semen Maydis, takes constant volume after 400g distilled water boils 10min and, to 500mL, cooling, uses filtered through gauze extracting juice；The manufacture method of Radix Dauci Sativae juice is fresh carrot chopping, takes constant volume after 400g distilled water boils 10min and, to 500mL, cooling, uses filtered through gauze extracting juice；
(2) material: be called 1L according to the culture medium cumulative volume that need to prepare, weigh corn starch and the brown sugar of 10-30g, corn starch is 1:2 with the mass ratio of brown sugar, the bean milk of 30-50g and egg yolk, bean milk is 1:2 with the mass ratio of egg yolk, the corn juice of 120-180mL, the medicated beer of 120-180mL and the Radix Dauci Sativae juice of 70-130mL, be positioned over load weighted material in beaker；
(3) heating for dissolving: add distilled water in beaker to 700mL, then beaker is placed on asbestos gauge, little fire heating, and stirs with Glass rod, be supplemented with distilled water after material is completely dissolved in beaker to 1L；
(4) adjusting pH: the pH of detection culture medium, if pH meta-acid, the NaOH that can drip l mol/L regulates pH, stirring while adding, and uses pH detection paper at any time, until pH value reaches 6.8；If meta-alkali, then it is adjusted pH with the HCl of l mol/L；
(5) filter:Use filter paper filtering；
(6) subpackage: the culture medium funnel of preparation is distributed in triangular flask；
(7) add tampon: the tampon that triangle bottleneck makes of non-absorbent cotton beyond the Great Wall, make tampon overall length 3/5 fill in bottleneck；
(8) wrapping: culture medium be sub-packed in triangular flask, one layer of kraft paper of the tampon outsourcing of triangular flask or double-deck newspaper, tie with rope；
(9) sterilizing: by above-mentioned cultivation based on 121.3 DEG C of moist heat sterilization 20min.
In described step (2), it is 1L according to the culture medium cumulative volume that need to prepare, weigh corn starch and the brown sugar of 20g, corn starch is 1:2 with the mass ratio of brown sugar, the corn juice that mass ratio is 1:2,150mL of the bean milk of 40g and egg yolk, bean milk and egg yolk, the medicated beer of 150mL and the Radix Dauci Sativae juice of 100mL, be positioned over load weighted material in beaker.
The invention has the beneficial effects as follows:
1), all raw materials be conveniently easy to get, just can buy in supermarket, manufacture method is the most uncomplicated；
2), cost relatively low, Carnis Bovis seu Bubali cream, peptone in conventional formulation are the most expensive, and all items in inventive formulation is the most cheap.
Detailed description of the invention
Embodiment 1
A kind of lactic acid bacteria enriched medium, lactic acid bacteria enriched medium described in preparation 1L, consist of the following composition: the corn starch of 20g and brown sugar, corn starch is 1:2 with the mass ratio of brown sugar, the corn juice that mass ratio is 1:2,150mL of the bean milk of 40g and egg yolk, bean milk and egg yolk, the medicated beer of 150mL and the Radix Dauci Sativae juice of 100mL, remaining complements to 1L with distilled water.
The manufacture method of a kind of lactic acid bacteria enriched medium, comprises the following steps:
(1) pretreatment of raw material: the manufacture method of bean milk is to take 50g Semen Glycines soy bean milk making machine to wear into bean milk, with distilled water constant volume to 500g；Egg yolk directly isolated from raw egg；The manufacture method of corn juice is that seed shelled by fresh Semen Maydis, takes constant volume after 400g distilled water boils 10min and, to 500mL, cools down, with 8 layers of filtered through gauze extracting juice；The manufacture method of Radix Dauci Sativae juice is fresh carrot chopping, takes constant volume after 400g distilled water boils 10min and, to 500mL, cools down, with 8 layers of filtered through gauze extracting juice；
(2) material: be called 1L according to the culture medium cumulative volume that need to prepare, weigh corn starch and the brown sugar of 20g, corn starch is 1:2 with the mass ratio of brown sugar, the bean milk of 40g and egg yolk, bean milk is 1:2 with the mass ratio of egg yolk, the corn juice of 150mL, the medicated beer of 150mL and the Radix Dauci Sativae juice of 100mL, be positioned over load weighted material in beaker；
(3) heating for dissolving: add distilled water in beaker to 700mL, then beaker is placed on asbestos gauge, little fire heating, and stirs with Glass rod, be supplemented with distilled water after material is completely dissolved in beaker to 1L；
(4) adjusting pH: the pH of detection culture medium, if pH meta-acid, the NaOH that can drip l mol/L regulates pH, stirring while adding, and uses pH detection paper at any time, until pH value reaches 6.8；
(5) filter:Use filter paper filtering；
(6) subpackage: the culture medium funnel of preparation is distributed in triangular flask；
(7) add tampon: the tampon that triangle bottleneck makes of non-absorbent cotton beyond the Great Wall, make tampon overall length 3/5 fill in bottleneck；
(8) wrapping: culture medium be sub-packed in triangular flask, one layer of kraft paper of the tampon outsourcing of triangular flask or double-deck newspaper, tie with rope；
(9) sterilizing: by above-mentioned cultivation based on 121.3 DEG C of moist heat sterilization 20min.
It is 1.5 × 10 by the amount of being seeded initially7The bacteria suspension of CFU/mL is coated on culture medium flat plate, cultivates 5.5h through 40 DEG C, and result is 1.75 × 109CFU/mL。
Embodiment 2
A kind of lactic acid bacteria enriched medium, lactic acid bacteria enriched medium described in preparation 1L, consist of the following composition: the corn starch of 10g and brown sugar, corn starch is 1:2 with the mass ratio of brown sugar, the corn juice that mass ratio is 1:2,120mL of the bean milk of 30g and egg yolk, bean milk and egg yolk, the medicated beer of 120mL and the Radix Dauci Sativae juice of 70mL, remaining complements to 1L with distilled water.
The manufacture method of a kind of lactic acid bacteria enriched medium, comprises the following steps:
(1) pretreatment of raw material: the manufacture method of bean milk is to take 50g Semen Glycines soy bean milk making machine to wear into bean milk, with distilled water constant volume to 500g；Egg yolk directly isolated from raw egg；The manufacture method of corn juice is that seed shelled by fresh Semen Maydis, takes constant volume after 400g distilled water boils 10min and, to 500mL, cooling, uses filtered through gauze extracting juice；The manufacture method of Radix Dauci Sativae juice is fresh carrot chopping, takes constant volume after 400g distilled water boils 10min and, to 500mL, cooling, uses filtered through gauze extracting juice；
(2) material: be called 1L according to the culture medium cumulative volume that need to prepare, weigh corn starch and the brown sugar of 10g, corn starch is 1:2 with the mass ratio of brown sugar, the bean milk of 30g and egg yolk, bean milk is 1:2 with the mass ratio of egg yolk, the corn juice of 120mL, the medicated beer of 120mL and the Radix Dauci Sativae juice of 70mL, be positioned over load weighted material in beaker；
(3) heating for dissolving: add distilled water in beaker to 700mL, then beaker is placed on asbestos gauge, little fire heating, and stirs with Glass rod, be supplemented with distilled water after material is completely dissolved in beaker to 1L；
(4) adjusting pH: the pH of detection culture medium, if pH meta-acid, the NaOH that can drip l mol/L regulates pH, stirring while adding, and uses pH detection paper at any time, until pH value reaches 6.8；
(5) filter:Use filter paper filtering；
(6) subpackage: the culture medium funnel of preparation is distributed in triangular flask；
(7) add tampon: the tampon that triangle bottleneck makes of non-absorbent cotton beyond the Great Wall, make tampon overall length 3/5 fill in bottleneck；
(8) wrapping: culture medium be sub-packed in triangular flask, one layer of kraft paper of the tampon outsourcing of triangular flask or double-deck newspaper, tie with rope；
(9) sterilizing: by above-mentioned cultivation based on 121.3 DEG C of moist heat sterilization 20min.
It is 1.5 × 10 by the amount of being seeded initially7The bacteria suspension of CFU/mL is coated on culture medium flat plate, cultivates 5.5h through 40 DEG C, and result is 1.65 × 109CFU/mL。
Embodiment 3
A kind of lactic acid bacteria enriched medium, prepares lactic acid bacteria enriched medium described in 1L, consists of the following composition: the corn starch of 30g and brown sugar, the bean milk of 50g and egg yolk, the corn juice of 180mL, the medicated beer of 180mL and the Radix Dauci Sativae juice of 130mL, remaining complements to 1L with distilled water.
The manufacture method of a kind of lactic acid bacteria enriched medium, comprises the following steps:
(1) pretreatment of raw material: the manufacture method of bean milk is to take 50g Semen Glycines soy bean milk making machine to wear into bean milk, with distilled water constant volume to 500g；Egg yolk directly isolated from raw egg；The manufacture method of corn juice is that seed shelled by fresh Semen Maydis, takes constant volume after 400g distilled water boils 10min and, to 500mL, cooling, uses filtered through gauze extracting juice；The manufacture method of Radix Dauci Sativae juice is fresh carrot chopping, takes constant volume after 400g distilled water boils 10min and, to 500mL, cooling, uses filtered through gauze extracting juice；
(2) material: be called 1L according to the culture medium cumulative volume that need to prepare, weigh corn starch and the brown sugar of 30g, corn starch is 1:2 with the mass ratio of brown sugar, the bean milk of 50g and egg yolk, bean milk is 1:2 with the mass ratio of egg yolk, the corn juice of 180mL, the medicated beer of 180mL and the Radix Dauci Sativae juice of 130mL, be positioned over load weighted material in beaker；
(3) heating for dissolving: add distilled water in beaker to 700mL, then beaker is placed on asbestos gauge, little fire heating, and stirs with Glass rod, be supplemented with distilled water after material is completely dissolved in beaker to 1L；
(4) adjusting pH: the pH of detection culture medium, if pH meta-acid, the NaOH that can drip l mol/L regulates pH, stirring while adding, and uses pH detection paper at any time, until pH value reaches 6.8；
(5) filter:Use filter paper filtering；
(6) subpackage: the culture medium funnel of preparation is distributed in triangular flask；
(7) add tampon: the tampon that triangle bottleneck makes of non-absorbent cotton beyond the Great Wall, make tampon overall length 3/5 fill in bottleneck；
(8) wrapping: culture medium be sub-packed in triangular flask, one layer of kraft paper of the tampon outsourcing of triangular flask or double-deck newspaper, tie with rope；
(9) sterilizing: by above-mentioned cultivation based on 121.3 DEG C of moist heat sterilization 20min.
It is 1.5 × 10 by the amount of being seeded initially7The bacteria suspension of CFU/mL is coated on culture medium flat plate, cultivates 5.5h through 40 DEG C, and result is 1.85 × 109CFU/mL。
Claims (3)
1. a lactic acid bacteria enriched medium, it is characterized in that: lactic acid bacteria enriched medium described in preparation 1L, consist of the following composition: the corn starch of 10-30g and brown sugar, the bean milk of 30-50g and egg yolk, the corn juice of 120-180mL, the medicated beer of 120-180mL and the Radix Dauci Sativae juice of 70-130mL, remaining complements to 1L with distilled water；Described corn starch and brown sugar are carbon source, and its ratio is corn starch: brown sugar=1:2；Described bean milk and egg yolk are nitrogen source, and its ratio is bean milk: egg yolk=1:2；Described Radix Dauci Sativae juice is to be shredded by fresh carrot, after boiling with distilled water, and the juice obtained by filtered through gauze.
2. the manufacture method of a lactic acid bacteria enriched medium, it is characterised in that: comprise the following steps:
(1) pretreatment of raw material: the manufacture method of bean milk is to take 50g Semen Glycines soy bean milk making machine to wear into bean milk, with distilled water constant volume to 500g；Egg yolk directly isolated from raw egg；The manufacture method of corn juice is that seed shelled by fresh Semen Maydis, takes constant volume after 400g distilled water boils 10min and, to 500mL, cooling, uses filtered through gauze extracting juice；The manufacture method of Radix Dauci Sativae juice is fresh carrot chopping, takes constant volume after 400g distilled water boils 10min and, to 500mL, cooling, uses filtered through gauze extracting juice；
(2) material: be called 1L according to the culture medium cumulative volume that need to prepare, weigh corn starch and the brown sugar of 10-30g, corn starch is 1:2 with the mass ratio of brown sugar, the bean milk of 30-50g and egg yolk, bean milk is 1:2 with the mass ratio of egg yolk, the corn juice of 120-180mL, the medicated beer of 120-180mL and the Radix Dauci Sativae juice of 70-130mL, be positioned over load weighted material in beaker；
(3) heating for dissolving: add distilled water in beaker to 700mL, then beaker is placed on asbestos gauge, little fire heating, and stirs with Glass rod, be supplemented with distilled water after material is completely dissolved in beaker to 1L；
(4) adjusting pH: the pH of detection culture medium, if pH meta-acid, the NaOH that can drip l mol/L regulates pH, stirring while adding, and uses pH detection paper at any time, until pH value reaches 6.8；If meta-alkali, then use l
The HCl of mol/L is adjusted pH；
(5) filter:Use filter paper filtering；
(6) subpackage: the culture medium funnel of preparation is distributed in triangular flask；
(7) add tampon: the tampon that triangle bottleneck makes of non-absorbent cotton beyond the Great Wall, make tampon overall length 3/5 fill in bottleneck；
(8) wrapping: culture medium be sub-packed in triangular flask, one layer of kraft paper of the tampon outsourcing of triangular flask or double-deck newspaper, tie with rope；
(9) sterilizing: by above-mentioned cultivation based on 121.3 DEG C of moist heat sterilization 20min.
The manufacture method of a kind of lactic acid bacteria enriched medium the most according to claim 2, it is characterized in that: in described step (2), it is 1L according to the culture medium cumulative volume that need to prepare, weigh the bean milk that mass ratio is 1:2,40g and the egg yolk of corn starch and the brown sugar of 20g, corn starch and brown sugar, bean milk is 1:2 with the mass ratio of egg yolk, the corn juice of 150mL, the medicated beer of 150mL and the Radix Dauci Sativae juice of 100mL, be positioned over load weighted material in beaker.
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