CN106045706A - Bacillus licheniformis liquid preparation and preparation method thereof - Google Patents
Bacillus licheniformis liquid preparation and preparation method thereof Download PDFInfo
- Publication number
- CN106045706A CN106045706A CN201610401581.9A CN201610401581A CN106045706A CN 106045706 A CN106045706 A CN 106045706A CN 201610401581 A CN201610401581 A CN 201610401581A CN 106045706 A CN106045706 A CN 106045706A
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- CN
- China
- Prior art keywords
- bacillus licheniformis
- preparation
- spore
- liquid preparation
- liquid
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a preparation method of a bacillus licheniformis liquid preparation. The bacillus licheniformis liquid preparation comprises fermentation broth of one, two or three of three kinds of bacillus licheniformis preserved in the ACCC (Agricultural Culture Collection of China) with the preservation numbers of ACCC 11080, ACCC 10613 and ACCC 11106, citric acid, amino acid, sodium benzoate and borax. The preparation method comprises the following steps: one, two or three of the bacillus licheniformis fermentation broth with the spore forming rate being equal to or higher than 90% and the spore number being higher than or equal to 5 billion/ml is taken, heated to 55-65 DEG C, kept for 20 min, and is drawn off when cooled to 40 DEG C; the auxiliaries are added, in terms of every 1,000 L of the fermentation broth, the auxiliaries comprising 0.5-1 kg of citric acid, 40-80 kg of amino acid, 5 kg of sodium benzoate and 1-2 kg of the borax are added, and the materials are subjected to stirring dissolution and vacuum concentration to 50%. The preparation method has the beneficial effects that the loss of the spore number is 15% or lower when the product is stored for more than 6 months, and the problems of proneness to bottle expansion, stink and quick loss of spores of the bacillus licheniformis liquid preparation are solved.
Description
Technical field
The present invention relates to the preparation method of a kind of Bacillus licheniformis liquid preparation, belong to microorganism field.
Background technology
Agricultural lichens bacillus preparation is because having raising fertilizer efficiency, promoting to absorb, exempt to deep plough, improve crop yield, improvement
The effects such as crop quality, promotion root growth, are increasingly welcome by user.Three kinds of lichens spore bars involved in the present invention
Bacterium is the three kinds of Bacillus licheniformis being preserved in Chinese agriculture Culture Collection, wherein, every kind of Bacillus licheniformis
Deposit number be respectively ACCC11080, ACCC10613, ACCC11106.
In Bacillus licheniformis body preparation involved in the present invention, metabolite relatively horn of plenty, is more beneficial for plant absorption,
But liquid preparation can make the recovery of lichens spore sprout, easily produce a large amount of gases during storing transport, occur that swollen bottle phenomenon is concurrent
Smelly, cause spore number to reduce more than 45%, spore loss cannot meet business demand soon.
Summary of the invention
The purpose of the present invention is contemplated to overcome the deficiencies in the prior art, it is provided that the Bacillus licheniformis liquid of a kind of easy preservation
Body preparation and preparation method thereof.
For achieving the above object, the present invention is by the following technical solutions:
A kind of Bacillus licheniformis liquid preparation, including being preserved in Chinese agriculture Culture Collection deposit number respectively
For three kinds of Bacillus licheniformis of ACCC11080, ACCC10613, ACCC11106 one or both or three kinds of fermentation liquids therein
With citric acid, aminoacid, sodium benzoate, Borax, it is characterized in that: every 500L Bacillus licheniformis liquid preparation 1-Han citric acid
2kg, aminoacid 80-160kg, sodium benzoate 5-10kg, Borax 2-4kg, Bacillus licheniformis spore rate 90%, spore number
9000000000/ml.
The preparation method of a kind of Bacillus licheniformis liquid preparation, Bacillus licheniformis liquid preparation includes being preserved in China
Agricultural Culture Collection deposit number is respectively three kinds of lichens buds of ACCC11080, ACCC10613, ACCC11106
Spore bacillus one or both or three kinds of fermentation liquids therein and citric acid, aminoacid, sodium benzoate, Borax, preparation method is: take
One of which or two or three the lichen bacillus ferments liquid, spore rate is 90%, and spore number 5,000,000,000/ml is heated to
55-65 DEG C, holding 20min, be down to when 40 DEG C put tank；Add adjuvant, be evaporated to 50% after stirring and dissolving, obtain lichens spore
Bacillus preparation；It is characterized in that: component and the content of described interpolation adjuvant is: the weight that every 1000L fermentation liquid adds adjuvant is: lemon
Lemon acid 0.5-1kg, aminoacid 40-80kg, sodium benzoate 5kg, Borax 1-2kg.
The present invention compared with prior art provides the benefit that: effect duration reaches more than 6 months, spore number loss less than 15%,
Solve Bacillus licheniformis liquid preparation and store easy swollen bottle during transport, the problem smelly, spore loss is fast.
Detailed description of the invention
Below in conjunction with specific embodiment, technical scheme is further described.
The lichen bacillus ferments liquid involved by invention refers to be preserved in Chinese agriculture Culture Collection
Deposit number is respectively three kinds of Bacillus licheniformis a kind of lichens bud therein of ACCC11080, ACCC10613, ACCC11106
Spore bacillus fermentation liquid or the lichen bacillus ferments liquid of two or three mixing.
As a example by a kind of ACCC11080 the lichen bacillus ferments liquid (hereinafter referred to as " fermentation liquid "), (two or three mixes
The Bacillus licheniformis liquid preparation preparation method closed is identical), traditional handicraft is prepared fermentation liquor technique and is: take fresh traditional handicraft
ACCC11080 the lichen bacillus ferments liquid of preparation, spore rate is 95%, and spore number is 6,300,000,000/ml；Every 1000L fermentation liquid subtracts
Pressure is concentrated into 500L, obtains Bacillus licheniformis liquid preparation.
After traditional handicraft prepares fermentation liquid placement 1 day, there is slight aerogenesis phenomenon, it will be seen that the bottle of dress liquid preparation has slightly
Swollen bottle phenomenon；After placing 5 days, hence it is evident that find out that the bottle bottom of dress liquid preparation rises cruelly with bottle cap.Open bottle, send substantially
Acid, stink, observe with microscope, finds that spore has 40-50% to change into thalline；After placing 8 days, observing thalline has 30% death,
Visual field endospore significantly reduces, and utilizing flat board detection method detection spore number is 7,200,000,000/ml；After placing 1 month, flat board is utilized to detect
Method detection spore number is only 5,600,000,000/ml, spore number loss more than 45%.
Embodiment 1
One Bacillus licheniformis liquid preparation of the present invention and preparation method thereof, takes ACCC11080 prepared by fresh traditional handicraft
The lichen bacillus ferments liquid, spore rate is 95%, and spore number is 6,300,000,000/ml；It is heated to 60 DEG C, keeps 20min, be down to 40 DEG C
Time put tank；Adding adjuvant, every 1000L fermentation liquid adds the weight of adjuvant and is: citric acid 1kg, aminoacid 80kg, sodium benzoate
5kg, Borax 2kg, be evaporated to 500L after stirring and dissolving, obtains Bacillus licheniformis liquid preparation.Detection spore number is 102
Hundred million/ml.
After the liquid preparation prepared by the present embodiment is placed 1 day, do not find that liquid bottle has swollen bottle phenomenon；Room temperature places 1
After Yue, finding no bottle phenomenon that rises, open bottle cap, do not smell pungent acid smell, microscope observes spore rate about 90%；
After room temperature is placed 3 months, finding no bottle phenomenon that rises, open bottle cap, do not smell pungent acid smell, microscope observes spore rate
About 90%, visual field endospore number does not has significant change, and utilizing flat board detection method detection viable count is 9,100,000,000/ml, and spore number damages
Lose 13.3%；Room temperature is placed 6 months, finds no bottle phenomenon that rises, opens bottle cap, does not smell pungent acid smell, and microscope is observed
Spore rate is about 90%, and visual field endospore number does not has significant change, and utilizing flat board detection method detection viable count is 8,800,000,000/ml, bud
Spore number loss 13.8%.
Embodiment 2
One Bacillus licheniformis liquid preparation of the present invention and preparation method thereof, takes ACCC11080 prepared by fresh traditional handicraft
The lichen bacillus ferments liquid, spore rate is 94%, and spore number is 6,000,000,000/ml, is heated to 60 DEG C, keeps 20min, is down to 40 DEG C
Time put tank；Adding adjuvant, every 1000L fermentation liquid adds the weight of adjuvant and is: citric acid 0.8kg, aminoacid 60kg, sodium benzoate
5kg, Borax 1.5kg, after stirring and dissolving, blood pressure lowering is concentrated into 500L, obtains Bacillus licheniformis liquid preparation.Detection spore number is 104
Hundred million/ml.
After the liquid preparation prepared by the present embodiment is placed 1 day, do not find that liquid bottle has swollen bottle phenomenon；Room temperature places 1
After Yue, finding no bottle phenomenon that rises, open bottle cap, do not smell pungent acid smell, microscope is observed spore rate and is regarded about 90%
In wild, spore number does not has significant change, and utilizing flat board detection method detection viable count is 9,600,000,000/ml, spore number loss 7.7%；Room temperature
After placing 3 months, find no bottle phenomenon that rises, open bottle cap, do not smell pungent acid smell, microscope field of view endospore
Number does not has significant change, and utilizing flat board detection method detection viable count is 9,000,000,000/ml, spore number loss 13.5%.Room temperature places 6
After Yue, finding no bottle phenomenon that rises, open bottle cap, do not smell pungent acid smell, microscope field of view endospore number does not has
Significant change, utilizing flat board detection method detection viable count is 8,800,000,000/ml, spore number loss 15.4%.
Embodiment 3
One Bacillus licheniformis liquid preparation of the present invention and preparation method thereof, takes ACCC11080 prepared by fresh traditional handicraft
The lichen bacillus ferments liquid, spore rate is 96%, and spore number is 6,700,000,000/ml, is heated to 55 DEG C, keeps 20min, is down to 40 DEG C
Time put tank；Adding adjuvant, every 1000L fermentation liquid adds the weight of adjuvant and is: citric acid 0.5kg, aminoacid 40kg, sodium benzoate
5kg, Borax 1kg, be evaporated to 500L after stirring and dissolving, obtains Bacillus licheniformis liquid preparation.Detection spore number is 122
Hundred million/ml.
After the liquid preparation prepared by the present embodiment is placed 1 day, do not find that liquid bottle has swollen bottle phenomenon；After placing 1 month,
Finding no bottle phenomenon that rises, open bottle cap, do not smell pungent odor, microscope observation spore rate, about 90%, utilizes flat board
Detection method detection viable count is 11,400,000,000/ml, spore number loss 6.7%；After placing 3 months, find no bottle phenomenon that rises, open bottle
Lid, does not smell pungent odor, and field of microscope endospore number does not has significant change, utilizes flat board detection method to detect viable count and is
10800000000/ml, spore number loss 11.5%.After placing 6 months, find no bottle phenomenon that rises, open bottle cap, do not smell pungent smelly
Taste, field of microscope endospore number does not has significant change, and utilizing flat board detection method detection viable count is 10,700,000,000/ml, and spore number damages
Lose 12.3%.
Product of the present invention stores up to more than 6 months, spore number loss less than 15%, solves Bacillus licheniformis liquid system
The easy swollen bottle of agent, the problem smelly, spore loss is fast, compared with traditional handicraft, spore stability significantly improves, and significantly improves
The product quality of Bacillus licheniformis liquid preparation.
Claims (2)
1. a Bacillus licheniformis liquid preparation, divides including being preserved in Chinese agriculture Culture Collection deposit number
Wei three kinds of Bacillus licheniformis of ACCC11080, ACCC10613, ACCC11106 one or both or three kinds of fermentations therein
Liquid and citric acid, aminoacid, sodium benzoate, Borax, is characterized in that: every 500L Bacillus licheniformis liquid preparation 1-Han citric acid
2kg, aminoacid 80-160kg, sodium benzoate 5-10kg, Borax 2-4kg, Bacillus licheniformis spore rate 90%, spore number
9000000000/ml.
2. a preparation method for Bacillus licheniformis liquid preparation, Bacillus licheniformis liquid preparation includes being preserved in China's agriculture
Industry Culture Collection deposit number is respectively three kinds of lichens spore of ACCC11080, ACCC10613, ACCC11106
Bacillus one or both or three kinds of fermentation liquids therein and citric acid, aminoacid, sodium benzoate, Borax, preparation method is: take it
In one or both or three kinds of the lichen bacillus ferments liquid, spore rate is 90%, and spore number 5,000,000,000/ml is heated to 55-
65 DEG C, holding 20min, be down to when 40 DEG C put tank；Add adjuvant, be evaporated to 50% after stirring and dissolving, obtain Bacillus licheniformis
Preparation；It is characterized in that: component and the content of described interpolation adjuvant is: the weight that every 1000L fermentation liquid adds adjuvant is: citric acid
0.5-1kg, aminoacid 40-80kg, sodium benzoate 5kg, Borax 1-2kg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610401581.9A CN106045706A (en) | 2016-06-11 | 2016-06-11 | Bacillus licheniformis liquid preparation and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610401581.9A CN106045706A (en) | 2016-06-11 | 2016-06-11 | Bacillus licheniformis liquid preparation and preparation method thereof |
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| CN106045706A true CN106045706A (en) | 2016-10-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610401581.9A Pending CN106045706A (en) | 2016-06-11 | 2016-06-11 | Bacillus licheniformis liquid preparation and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114395503A (en) * | 2021-12-06 | 2022-04-26 | 湖北工业大学 | Fermentation method for Bacillus licheniformis with high cell density and/or high spore quantity |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1584009A (en) * | 2004-06-02 | 2005-02-23 | 宁贵 | Fermenting liquid for feed with microbiological colonies |
| CN101294141A (en) * | 2007-04-28 | 2008-10-29 | 上海四季生物科技有限公司 | A set of living body microorganism preparations for preparing composite microorganism fertilizer and preparation method thereof |
| CN103553828A (en) * | 2013-11-12 | 2014-02-05 | 天津北洋百川生物技术有限公司 | Liquid compound biological fertilizer with long shelf life as well as preparation method and application thereof |
| CN103911314A (en) * | 2013-10-31 | 2014-07-09 | 领绿生物镇江有限公司 | Bacillus subtilis spore protective agent and usage method thereof |
-
2016
- 2016-06-11 CN CN201610401581.9A patent/CN106045706A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1584009A (en) * | 2004-06-02 | 2005-02-23 | 宁贵 | Fermenting liquid for feed with microbiological colonies |
| CN101294141A (en) * | 2007-04-28 | 2008-10-29 | 上海四季生物科技有限公司 | A set of living body microorganism preparations for preparing composite microorganism fertilizer and preparation method thereof |
| CN103911314A (en) * | 2013-10-31 | 2014-07-09 | 领绿生物镇江有限公司 | Bacillus subtilis spore protective agent and usage method thereof |
| CN103553828A (en) * | 2013-11-12 | 2014-02-05 | 天津北洋百川生物技术有限公司 | Liquid compound biological fertilizer with long shelf life as well as preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 刘志皋等: "《食品添加剂基础》", 31 January 1997, 中国轻工业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114395503A (en) * | 2021-12-06 | 2022-04-26 | 湖北工业大学 | Fermentation method for Bacillus licheniformis with high cell density and/or high spore quantity |
| CN114395503B (en) * | 2021-12-06 | 2023-08-22 | 湖北工业大学 | Fermentation method of bacillus licheniformis with high cell density and/or high spore amount |
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Application publication date: 20161026 |