CN102311902B - Novel culture method of Luzhou-flavor liquor pit mud - Google Patents
Novel culture method of Luzhou-flavor liquor pit mud Download PDFInfo
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- 238000012136 culture method Methods 0.000 title claims abstract description 25
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- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 42
- 241000894006 Bacteria Species 0.000 claims abstract description 35
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims abstract description 21
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims abstract description 21
- 239000001632 sodium acetate Substances 0.000 claims abstract description 21
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- 125000003118 aryl group Chemical group 0.000 claims description 35
- 208000035404 Autolysis Diseases 0.000 claims description 27
- 206010057248 Cell death Diseases 0.000 claims description 27
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- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 19
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- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 6
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- 238000009413 insulation Methods 0.000 claims description 6
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 3
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- 210000005253 yeast cell Anatomy 0.000 claims description 3
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- 239000004927 clay Substances 0.000 abstract description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 abstract 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 abstract 3
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 15
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- SHZIWNPUGXLXDT-UHFFFAOYSA-N ethyl hexanoate Chemical compound CCCCCC(=O)OCC SHZIWNPUGXLXDT-UHFFFAOYSA-N 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 3
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- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
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- 235000019634 flavors Nutrition 0.000 description 1
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Abstract
The invention discloses a novel culture method of Luzhou-flavor liquor pit mud and belongs to the field of liquor brewing. The novel culture method of Luzhou-flavor liquor pit mud comprises the following steps of carrying out one-grade culture by starter containing high-quality pit mud extract and caproic acid bacteria which are rejuvenated by a caproic acid bacteria synthesis culture solution and is preserved in a sandy soil tube, adding uniformly caproic acid bacteria to carry out second-grade and third-grade culture, adding dissolved 60g of dipotassium hydrogen phosphate, 60g of sodium acetate, 30g of magnesium sulfate and 1L of high-quality pit mud extract into a yeast autolytic culture solution to carry out forth-grade culture, adding dissolved 700g of dipotassium hydrogen phosphate,700g of sodium acetate, 350g of magnesium sulfate, 1kg of high-quality pit mud, 9kg of wheat koji powder and forth-grade starter into the yeast autolytic culture solution for fifth-grade culture, mixing well 40kg of clay, 60kg of sludge, 10kg of fresh vinasse, 10kg of wheat koji, 10kg of fresh yellow water, 1kg of bean cake powder, 1kg of high-quality pit mud, 0.2kg of dipotassium hydrogen phosphate, 0.2kg of sodium acetate, 0.1kg of magnesium sulfate and 4kg of 30 degrees of liquor by stirring, and carrying out culture for 30 days to obtain novel pit mud. The novel culture method of Luzhou-flavor liquor pit mud can be utilized for production of liquor and is an innovation.
Description
Technical field
Novel culture method of Luzhou-flavor liquor pit mud of the present invention relates to brewing technical field; Be particularly related to the technical field of cellar for storing things mud in the wine brewing; Be specifically related to the Novel culture method of Luzhou-flavor liquor pit mud technical field.
Background technology
With regard to the aromatic Chinese spirit technical field, as you know, produce aromatic Chinese spirit, cellar for storing things mud is the basis, and Daqu is power, and technique is crucial.Cellar for storing things mud in the fermentation vat is absolutely necessary, it is one of essential condition in the Production of Luzhou-flavor Liquor Technology, because cellar for storing things mud is various beneficial microorganism carriers and the habitats such as caproic acid bacteria, methanobacteria, butyric bacteria, what of these beneficial microorganism kinds and quantity, be the main standard of weighing pit mud quality, be related to the quality of beneficial microorganism enrichment (vigorous) and decay, aromatic Chinese spirit quality and success or failure, the quality of aromatic Chinese spirit product is played vital effect; Thus, do not have good cellar for storing things mud, just can not produce superior Luzhou-flavor quality liquor.Simultaneously, the main body flavour ingredient ethyl hexanoate of aromatic Chinese spirit is to produce during the fermentation the effect of ethanol (alcohol part) by esterifying liquid by caproic acid bacteria meta-bolites (caproic acid) and wine unstrained spirits in the mud of cellar for storing things to generate; So the keying action on mud basis, cellar for storing things has just significantly manifested out.At present, although the cellar for storing things mud nutritive ingredient that each wine industry production unit is cultivated is had nothing in common with each other, but ubiquitous deficiency, defective and drawback are: still continue to continue to use tradition and fall behind cellar mud production technique, its nutritive ingredient shortcoming is scientific and reasonable, abundant not, balanced not, can't be complementary, it is not because new cellar for storing things mud has ester fragrance, and store the foul smell that mud has hydrogen sulfide, and shortcoming is on affecting the research trial and the implementation that are perfume, taste composition generation and quality liquor yield rate of wine product; Because lacking the required effective microorganisms of production aromatic Chinese spirit in the mud of cellar for storing things preponderates, cause the milk-acid bacteria amount reproduction, the lactic acid that produces generates lactic acid salt with iron, the calcium ion of storing in the mud, cause that cellar for storing things mud hardens, changed cellar for storing things mud composition, the required effective microorganisms of production aromatic Chinese spirit is not perched and enrichment environment, the aromatic Chinese spirit quality percentage is significantly descended.In view of contriver's expertise with abundant working experience and to the excelsior unremitting pursue of cause, the present invention is exactly on conscientiously and fully investigation, understanding, analysis, the existing known technology of summary and present situation basis, succeeds in developing to take " Novel culture method " gordian technique.
Summary of the invention
The present invention takes " Novel culture method " gordian technique, with the sixth of the twelve Earthly Branches acid bacterium rejuvenation of the synthetic nutrient solution of caproic acid bacteria with preservation of sand tube, carry out the one-level inoculation culture with the quality pit mud diffusion juice as seed, add successively the synthetic nutrient solution of caproic acid bacteria and do two, three grades of enlarged culturing, add dipotassium hydrogen phosphate and each 60 gram of sodium acetate of adding dissolving in the yeast autolysis nutrient solution, sal epsom 30 grams, described quality pit mud diffusion juice 1L 3. does the level Four enlarged culturing, dipotassium hydrogen phosphate and each 700 gram of sodium acetate of adding dissolving in the yeast autolysis nutrient solution, sal epsom 350 grams, 1 kilogram of quality pit mud, 9 kilograms in wheat koji powder, the level Four seed is done the Pyatyi enlarged culturing, with 40 kilograms of clays, 60 kilograms in mud, each 10 kilograms in fresh vinasse and wheat koji and fresh yellow water and Pyatyi seed, each 1 kilogram of soybean cake powder and quality pit mud, each 0.2 kilogram of dipotassium hydrogen phosphate and sodium acetate, 0.1 kilogram in sal epsom, 30 spend 4 kilograms of liquor turns the novel cellar for storing things mud that aromatic Chinese spirit was made in 35-37 ℃ of cultivation of even insulation in 30 days.
1., take " Novel culture method " gordian technique the purpose that reaches by the present invention is:, provide Novel culture method of Luzhou-flavor liquor pit mud; 2., make cellar for storing things mud break away from the production technique that tradition falls behind; 3., make the nutritive ingredient of cellar for storing things mud scientific and reasonable, abundant, balanced, complementary, be fragrant, taste compound and generate to further investigate and test and implementation is put into practice affect wine product; The breeding of lactic acid bacteria inhibiting suppresses lactic acid and iron, calcium ion generation lactic acid salt, suppresses cellar for storing things mud and hardens, and builds effective microorganisms and perches and enrichment environment; 4., build that wine product is build environment fragrant, taste compound in the mud of cellar for storing things, be that good condition is created in the production of aromatic Chinese spirit, be fragrant, taste compound and create good condition for wine product generates; 5., make pit mud culture realize stdn, standardization, mass-producing, and be convenient to implement, effect stability is reliable; 6., make pit mud culture under scientific and reasonable prerequisite, draw materials conveniently, avoid waste, saving resource, reduction cost, be beneficial to wide popularization and application.
For achieving the above object, technical scheme provided by the invention is:
A kind of technical scheme of Novel culture method of Luzhou-flavor liquor pit mud is:
1., preparation substratum: dipotassium hydrogen phosphate 0.4g, sodium acetate 5g, ammonium sulfate 0.5g, sal epsom 0.2g, yeast extract paste 5g, tap water 1000ml through after the cooling in 30 minutes of 0.1Mpa pressure sterilization, under aseptic condition, add dehydrated alcohol 20ml, calcium carbonate 10g behind the mixed dissolution, that cellar for storing things mud diffusion juice 100ml is prepared into the synthetic nutrient solution of caproic acid bacteria is for subsequent use;
2., preparation yeast autolysis nutrient solution: get 200 kilograms of fresh vinasses, add 800 liters in water, white sugar is 10 kilograms again, being cooled to 1 kilogram of interpolation has activated after 30-32 ℃ active dry yeast after boiling cultivates after 24 hours and product temperature to be increased to 37 ℃ of maintenances 48 hours, make it the yeast cell self-dissolving, it is for subsequent use to obtain the yeast autolysis nutrient solution;
3., cellar for storing things mud diffusion juice processes: get quality pit mud 10 grams, adding 90 milliliters in water, to obtain the quality pit mud diffusion juice after 80 ℃ of insulations are processed 10 minutes for subsequent use as seed;
4., preservation of sand tube rejuvenation of spawn: after the thin neck test tube that will be vaccinated, plug, preservation of sand tube bacterial classification are sterilized with alcohol, post label and put into sterilisable chamber, two described preservation of sand tube bacterial classifications are accessed described thin neck in vitro, add the synthetic nutrient solution of described caproic acid bacteria 1. to the thin neck of test tube place, plug shakes up beyond the Great Wall, and obtained preservation of sand tube rejuvenation of spawn seed liquor in 35-37 ℃ of heat insulating culture 7-9 days for subsequent use;
5., the first order seed inoculation culture: use thin neck test tube, the first, the described preservation of sand tube rejuvenation of spawn seed liquor that rejuvenation is good is inoculated inoculum size 10% by above-mentioned method 4.; The second, also inoculating by above-mentioned method 4. as seed through the described quality pit mud diffusion juice 3. of 80 ℃ of processing in 10 minutes, it is for subsequent use that the seed in first, second described thin neck test tube is cultivated 7 days acquisition first order seeds respectively at 35-37 ℃;
6., secondary seed enlarges inoculation culture: use 150 ml flasks, with the synthetic nutrient solution of 150 ml flasks, plug, described caproic acid bacteria, cultivate ripe described 5. in first, second first order seed after the alcohol sterilization, put into sterilisable chamber, first order seed in described first, second thin neck test tube is accessed respectively in 150 ml flasks, add the synthetic nutrient solution of caproic acid bacteria to full bottle place, after shaking up with S type Glass tubing plug beyond the Great Wall, it is for subsequent use that the inherent 0.6Mpa/Hg of the glass evacuated moisture eliminator of packing into obtained secondary seed in 7 days in 35-37 ℃ of heat insulating culture;
7., three grades of seeds enlarge inoculation culture: use the long-neck Florence flask, with the synthetic nutrient solution of 1000 milliliters of long-neck Florence flasks, plug, described caproic acid bacteria, cultivate ripe described secondary seed 6. and after the alcohol sterilization, put into sterilisable chamber, described first, second secondary seed is mixed in the described long-neck Florence flask of 1000 milliliters of accesses, add the synthetic nutrient solution of described caproic acid bacteria to bottle everywhere, after plug shook up beyond the Great Wall, it was for subsequent use to obtain three grades of seeds in 7 days in 35-37 ℃ of heat insulating culture;
8., the level Four seed enlarges inoculation culture: use the Ka Shi tank, add dipotassium hydrogen phosphate 60 grams that dissolved, sodium acetate 60 grams, sal epsom 30 grams in ripe described 9 kilograms of yeast autolysis nutrient solutions 2. with cultivating, accessing in described three grades of seeds 7. behind the described quality pit mud diffusion juice 1L 3. that processed in 10 minutes through 80 ℃, put into two bottles of described Ka Shi tanks, add described yeast autolysis nutrient solution to tank everywhere, after shaking up with S type Glass tubing plug beyond the Great Wall, it is for subsequent use to obtain the level Four seed in 7 days in 35-37 ℃ of heat insulating culture;
9., the Pyatyi seed enlarges inoculation culture: use vat, dipotassium hydrogen phosphate 700 grams, the sodium acetate 700 that interpolation has been dissolved in ripe described 90 kilograms of yeast autolysis nutrient solutions 2. restrains, sal epsom 350 restrains with cultivating, 1 kilogram of quality pit mud, 9 kilograms in wheat koji powder through heating and keeping 10 minutes to 90 ℃, put into described vat, access again described level Four seed 8., add described yeast autolysis nutrient solution to cylinder everywhere, beat after consumption shakes up, with the plastic cloth sealing, it is for subsequent use to obtain the Pyatyi seed in 9 days in 35-37 ℃ of heat insulating culture;
10., close mud and cultivate the novel cellar for storing things mud of aromatic Chinese spirit: closing mud the day before yesterday will be closing the mud place, the pit mud culture pond, close the mud required equipment, the required starting material of the novel pit mud culture of instrument and aromatic Chinese spirit are prepared to put in place, close the mud batching by 40 kilograms of clays, 60 kilograms in mud, 10 kilograms of fresh vinasses, 10 kilograms of wheat kojis, 1 kilogram of soybean cake powder, 1 kilogram of quality pit mud, 0.2 kilogram of dipotassium hydrogen phosphate, 0.1 kilogram in sal epsom, 0.2 kilogram of sodium acetate, 10 kilograms of fresh yellow waters, 4 kilograms of 30 degree liquor, 10 kilograms of compositions of described Pyatyi seed 9., the above-mentioned mud batching of closing is drawn in and closed mud field boundary ground, after angling spade to turn evenly with three teeth, application is closed the mud machine and is squeezed in the described pit mud culture pond, be incubated 35-37 ℃ and cultivated 30 days, be prepared into the novel cellar for storing things mud of aromatic Chinese spirit.
Described Novel culture method of Luzhou-flavor liquor pit mud, the active dry yeast that has activated described in the described yeast autolysis nutrient solution are that 2% syrup of active dry yeast and 10 times of active dry yeast weight activates 3-4 hour under 30-32 ℃ of temperature.
Described Novel culture method of Luzhou-flavor liquor pit mud, described 1. to 10. preparation quantity be basic quantity, its actual quantity is determined for increasing in proportion according to actual needs on the basis of basic quantity.
Described Novel culture method of Luzhou-flavor liquor pit mud is put on the fermentation vat wall of aromatic Chinese spirit and ground with the novel cellar for storing things mash of described aromatic Chinese spirit at the end, for the production fermentation of aromatic Chinese spirit ready.
Owing to adopted technical scheme provided by the invention; Because the present invention has adopted " Novel culture method " gordian technique; Since the present invention with the synthetic nutrient solution rejuvenation preservation of sand tube of caproic acid bacteria the sixth of the twelve Earthly Branches the acid bacterium and the quality pit mud diffusion juice do one-level as seed and cultivate, all add the synthetic nutrient solution of caproic acid bacteria and do two, three grades of cultivations, add dipotassium hydrogen phosphate and each 60 gram of sodium acetate of adding dissolving in the yeast autolysis nutrient solution, sal epsom 30 grams, quality pit mud diffusion juice 1L does level Four and cultivates, dipotassium hydrogen phosphate and each 700 gram of sodium acetate of adding dissolving in the yeast autolysis nutrient solution, sal epsom 350 grams, 1 kilogram of quality pit mud, 9 kilograms in wheat koji powder, the level Four seed is done Pyatyi and is cultivated, with 40 kilograms of clays, 60 kilograms in mud, each 10 kilograms in fresh vinasse and wheat koji and fresh yellow water and Pyatyi seed, each 1 kilogram of soybean cake powder and quality pit mud, each 0.2 kilogram of dipotassium hydrogen phosphate and sodium acetate, 0.1 kilogram in sal epsom, 30 spend 4 kilograms of liquor turns 30 days new cellars for storing things of one-tenth of 35-37 ℃ of cultivation of even insulation mud.Thereby so that the present invention with the beneficial effect that existing known technology is compared acquisition is:
" Novel culture method of Luzhou-flavor liquor pit mud " 1, is provided.
2, make pit mud culture break away from the production technique of traditional backwardness.
3, make the nutritive ingredient of cellar for storing things mud scientific and reasonable, abundant, balanced, complementary.
4, have in the mud of novel cellar for storing things and affect wine product and be fragrant, taste compound and generate required nutritive ingredient, obtained the beneficial effect that existing known technology can't be compared.
5, the effectively breeding of lactic acid bacteria inhibiting of novel cellar for storing things mud suppresses lactic acid and iron, calcium ion generation lactic acid salt, can suppress to store hardening of mud, has built that effective microorganisms is perched and enrichment environment; The taste that generates ester perfume (or spice) for wine product has been created good condition; Obtained the beneficial effect that existing known technology can't be compared.
6, make novel cellar for storing things mud under scientific and reasonable prerequisite, realize stdn, standardization, mass-producing, and be convenient to implement, effect stability is reliable, obtained the beneficial effect that existing known technology can't be compared.
7, owing to be provided with soybean cake powder among the present invention, so that the present invention has possessed the nutrition source of vegetable-protein, create good nutritional condition for the growth of the caproic acid bacteria in the pit mud culture, obtained the beneficial effect that existing known technology can't be compared.
8, owing to be provided with liquor among the present invention, thereby obtained pit mud culture is played initiation and is the beneficial effect of caproic acid growth increase carbon source.
9, be provided with inorganic salt solution owing among the present invention, thereby obtained to provide for caproic acid growth in the pit mud culture beneficial effect of trace element and raising wine quality.
10, owing to be provided with quality pit mud among the present invention, thereby obtained intrinsic caproic acid bacteria is grown in the quality pit mud micro-growth factor and beneficial microbe colony, can play initiation to pit mud culture, and the beneficial effect that takes full advantage of trace element in the quality pit mud.
11, be provided with yellow clay owing among the present invention, thereby obtain the beneficial effect as the various nutritive ingredient carrier functions of carrying.
12, owing to be provided with mud among the present invention, in the same beneficial effect while that obtains as the various nutritive ingredient carrier functions of carrying, mud not only nutrition itself is fully utilized, and it has the multiple beneficial microorganism species, to pit mud culture enrichment beneficial microbe colony dominate, obtained the beneficial effect that existing known technology can't be compared.
13, the cultivation of novel cellar for storing things mud is drawn materials under scientific and reasonable prerequisite conveniently, it is very convenient all to draw materials such as various starting material such as yeast nutrition liquid, inorganic salt, soybean cake powder, yellow clay, mud (mud in the lotus root pond), has also avoided simultaneously unnecessary waste, saving resource, reduction cost, has been beneficial to wide popularization and application.
Description of drawings
Figure of description is the technical process window synoptic diagram of the specific embodiment of the invention.
Embodiment
Embodiment one
Below in conjunction with Figure of description, the present invention is described in detail.Shown in Figure of description, particular content is as follows:
A kind of Novel culture method of Luzhou-flavor liquor pit mud,
1., preparation substratum: dipotassium hydrogen phosphate 0.4g, sodium acetate 5g, ammonium sulfate 0.5g, sal epsom 0.2g, yeast extract paste 5g, tap water 1000ml through after the cooling in 30 minutes of 0.1Mpa pressure sterilization, under aseptic condition, add dehydrated alcohol 20ml, calcium carbonate 10g behind the mixed dissolution, that cellar for storing things mud diffusion juice 100ml is prepared into the synthetic nutrient solution of caproic acid bacteria is for subsequent use;
2., preparation yeast autolysis nutrient solution: get 200 kilograms of fresh vinasses, add 800 liters in water, white sugar is 10 kilograms again, being cooled to 1 kilogram of interpolation has activated after 30-32 ℃ active dry yeast after boiling cultivates after 24 hours and product temperature to be increased to 37 ℃ of maintenances 48 hours, make it the yeast cell self-dissolving, it is for subsequent use to obtain the yeast autolysis nutrient solution;
3., cellar for storing things mud diffusion juice processes: get quality pit mud 10 grams, adding 90 milliliters in water, to obtain the quality pit mud diffusion juice after 80 ℃ of insulations are processed 10 minutes for subsequent use as seed;
4., preservation of sand tube rejuvenation of spawn: after the thin neck test tube that will be vaccinated, plug, preservation of sand tube bacterial classification are sterilized with alcohol, post label and put into sterilisable chamber, two described preservation of sand tube bacterial classifications are accessed described thin neck in vitro, add the synthetic nutrient solution of described caproic acid bacteria 1. to the thin neck of test tube place, plug shakes up beyond the Great Wall, and obtained preservation of sand tube rejuvenation of spawn seed liquor in 35-37 ℃ of heat insulating culture 7-9 days for subsequent use;
5., the first order seed inoculation culture: use thin neck test tube, the first, the described preservation of sand tube rejuvenation of spawn seed liquor that rejuvenation is good is inoculated inoculum size 10% by above-mentioned method 4.; The second, also inoculating by above-mentioned method 4. as seed through the described quality pit mud diffusion juice 3. of 80 ℃ of processing in 10 minutes, it is for subsequent use that the seed in first, second described thin neck test tube is cultivated 7 days acquisition first order seeds respectively at 35-37 ℃;
6., secondary seed enlarges inoculation culture: use 150 ml flasks, with the synthetic nutrient solution of 150 ml flasks, plug, described caproic acid bacteria, cultivate ripe described 5. in first, second first order seed after the alcohol sterilization, put into sterilisable chamber, first order seed in described first, second thin neck test tube is accessed respectively in 150 ml flasks, add the synthetic nutrient solution of caproic acid bacteria to full bottle place, after shaking up with S type Glass tubing plug beyond the Great Wall, it is for subsequent use that the inherent 0.6Mpa/Hg of the glass evacuated moisture eliminator of packing into obtained secondary seed in 7 days in 35-37 ℃ of heat insulating culture;
7., three grades of seeds enlarge inoculation culture: use the long-neck Florence flask, with the synthetic nutrient solution of 1000 milliliters of long-neck Florence flasks, plug, described caproic acid bacteria, cultivate ripe described secondary seed 6. and after the alcohol sterilization, put into sterilisable chamber, described first, second secondary seed is mixed in the described long-neck Florence flask of 1000 milliliters of accesses, add the synthetic nutrient solution of described caproic acid bacteria to bottle everywhere, after plug shook up beyond the Great Wall, it was for subsequent use to obtain three grades of seeds in 7 days in 35-37 ℃ of heat insulating culture;
8., the level Four seed enlarges inoculation culture: use the Ka Shi tank, add dipotassium hydrogen phosphate 60 grams that dissolved, sodium acetate 60 grams, sal epsom 30 grams in ripe described 9 kilograms of yeast autolysis nutrient solutions 2. with cultivating, accessing in described three grades of seeds 7. behind the described quality pit mud diffusion juice 1L 3. that processed in 10 minutes through 80 ℃, put into two bottles of described Ka Shi tanks, add described yeast autolysis nutrient solution to tank everywhere, after shaking up with S type Glass tubing plug beyond the Great Wall, it is for subsequent use to obtain the level Four seed in 7 days in 35-37 ℃ of heat insulating culture;
9., the Pyatyi seed enlarges inoculation culture: use vat, dipotassium hydrogen phosphate 700 grams, the sodium acetate 700 that interpolation has been dissolved in ripe described 90 kilograms of yeast autolysis nutrient solutions 2. restrains, sal epsom 350 restrains with cultivating, 1 kilogram of quality pit mud, 9 kilograms in wheat koji powder through heating and keeping 10 minutes to 90 ℃, put into described vat, access again described level Four seed 8., add described yeast autolysis nutrient solution to cylinder everywhere, beat after consumption shakes up, with the plastic cloth sealing, it is for subsequent use to obtain the Pyatyi seed in 9 days in 35-37 ℃ of heat insulating culture;
10., close mud and cultivate the novel cellar for storing things mud of aromatic Chinese spirit: closing mud the day before yesterday will be closing the mud place, the pit mud culture pond, close the mud required equipment, the required starting material of the novel pit mud culture of instrument and aromatic Chinese spirit are prepared to put in place, close the mud batching by 40 kilograms of clays, 60 kilograms in mud, 10 kilograms of fresh vinasses, 10 kilograms of wheat kojis, 1 kilogram of soybean cake powder, 1 kilogram of quality pit mud, 0.2 kilogram of dipotassium hydrogen phosphate, 0.1 kilogram in sal epsom, 0.2 kilogram of sodium acetate, 10 kilograms of fresh yellow waters, 4 kilograms of 30 degree liquor, 10 kilograms of compositions of described Pyatyi seed 9., the above-mentioned mud batching of closing is drawn in and closed mud field boundary ground, after angling spade to turn evenly with three teeth, application is closed the mud machine and is squeezed in the described pit mud culture pond, be incubated 35-37 ℃ and cultivated 30 days, be prepared into the novel cellar for storing things mud of aromatic Chinese spirit.
Described Novel culture method of Luzhou-flavor liquor pit mud, the active dry yeast that has activated described in the described yeast autolysis nutrient solution are that 2% syrup of active dry yeast and 10 times of active dry yeast weight activates 3-4 hour under 30-32 ℃ of temperature.
Described Novel culture method of Luzhou-flavor liquor pit mud, described 1. to 10. preparation quantity be basic quantity, its actual quantity is determined for increasing in proportion according to actual needs on the basis of basic quantity.
Described Novel culture method of Luzhou-flavor liquor pit mud is put on the fermentation vat wall of aromatic Chinese spirit and ground with the novel cellar for storing things mash of described aromatic Chinese spirit at the end, for the production fermentation of aromatic Chinese spirit ready.
In above-mentioned specific implementation process: implement with 30,31,32 ℃ respectively boiling rear cooling in the described preparation yeast autolysis nutrient solution; To implementing to cultivate 7,8,9 days respectively with 35,36,37 ℃ of insulations respectively in the described preservation of sand tube rejuvenation of spawn; Described first order seed inoculation culture, secondary seed are enlarged inoculation culture, three grades of seeds to be enlarged inoculation culture, level Four seeds and enlarges to cultivate with 35,36,37 ℃ respectively in the inoculation culture and implemented in 7 days; Implemented in 9 days cultivating with 35,36,37 ℃ respectively in the described Pyatyi seed expansion inoculation culture; The described mud that closes is cultivated in the novel cellar for storing things mud of aromatic Chinese spirit respectively to be incubated 35,36,37 ℃ and cultivated and implemented in 30 days; To implementing under 30,31,32 ℃ of temperature, to activate 3,3.5,4 hours respectively in the prepared yeast autolysis nutrient solution; To described 1. to 10. preparation quantity be that basic quantity is implemented to enlarge 1,2,3,5,7,8,10,12,15,20,25,30,35,40,45,50,55,60,65,70,75,80 times respectively; All received the good result of expection.
Embodiment two
Implement by embodiment one, just:
1--5 level seed enlarged culturing conditional request sees the following form
Embodiment three
Implement by embodiment one, just on the basis of embodiment one, carry out multiple multiple enforcement and carry out simultaneously the detection of indices to dwindle 30,25,20,15,10,5 times respectively, obtained the good result of expection; So that the present invention has sturdy small-scale test, expanding test basis.
Embodiment four
Implement by embodiment one, just on the basis of embodiment one, carry out multiple multiple enforcement and carry out simultaneously the detection of indices to enlarge 1,3,5,7,10,15,20,25,30 times respectively, obtained the good result of expection; So that the present invention has sturdy again expanding test, Production Type Test basis, for specifically in wine brewing is produced application implementation established solid basis.
Embodiment five
Use embodiment one, two, three, the four novel cellar for storing things mud of making, through having gone the industrial experimentation of aromatic Chinese spirit, the novel cellar for storing things mash that is about to described aromatic Chinese spirit is solid in the fermentation vat of aromatic Chinese spirit, industrial experimentation through the row aromatic Chinese spirit, because the present invention has played vital role to content and the eurythmy of the trace ingredientss such as aromatic Chinese spirit four large esters, and having determined quality and the style and features of aromatic Chinese spirit, the ethyl hexanoate that embodies ester perfume (or spice) in the wine product of the present invention is 309.17mg/100mL, the 188.3mg/100mL of contrast test group exceeds 120.8mg/100mL.Obtained the good result of expection through property examination on probation.
In implementation of the present invention, to measure cultivating ripe novel pit mud quality composition, measurement result sees the following form:
Cultivate as a result table 100 time batch mean value of ripe novel pit mud quality Determination of composition
The above is preferred embodiment of the present invention only, is not the present invention is done any pro forma restriction; The those of ordinary skill of all industry, but all shown in the by specification accompanying drawing and the above, and implement swimmingly the present invention; But the equivalent variations of making differentiation not breaking away from technical solution of the present invention is equivalent embodiment of the present invention, all still belongs to technical scheme of the present invention.
Claims (4)
1. Novel culture method of Luzhou-flavor liquor pit mud is characterized in that:
1., preparation substratum: dipotassium hydrogen phosphate 0.4g, sodium acetate 5g, ammonium sulfate 0.5g, sal epsom 0.2g, yeast extract paste 5g, tap water 1000ml through after the cooling in 30 minutes of 0.1Mpa pressure sterilization, under aseptic condition, add dehydrated alcohol 20ml, calcium carbonate 10g behind the mixed dissolution, that cellar for storing things mud diffusion juice 100ml is prepared into the synthetic nutrient solution of caproic acid bacteria is for subsequent use;
2., preparation yeast autolysis nutrient solution: get 200 kilograms of fresh vinasses, add 800 liters in water, white sugar is 10 kilograms again, being cooled to 1 kilogram of interpolation has activated after 30-32 ℃ active dry yeast after boiling cultivates after 24 hours and product temperature to be increased to 37 ℃ of maintenances 48 hours, make it the yeast cell self-dissolving, it is for subsequent use to obtain the yeast autolysis nutrient solution;
3., cellar for storing things mud diffusion juice processes: get quality pit mud 10 grams, adding 90 milliliters in water, to obtain the quality pit mud diffusion juice after 80 ℃ of insulations are processed 10 minutes for subsequent use as seed;
4., preservation of sand tube rejuvenation of spawn: after the thin neck test tube that will be vaccinated, plug, preservation of sand tube bacterial classification are sterilized with alcohol, post label and put into sterilisable chamber, two described preservation of sand tube bacterial classifications are accessed described thin neck in vitro, add the synthetic nutrient solution of described caproic acid bacteria 1. to the thin neck of test tube place, plug shakes up beyond the Great Wall, and obtained preservation of sand tube rejuvenation of spawn seed liquor in 35-37 ℃ of heat insulating culture 7-9 days for subsequent use;
5., the first order seed inoculation culture: use thin neck test tube, the first, the described preservation of sand tube rejuvenation of spawn seed liquor that rejuvenation is good is inoculated inoculum size 10% by above-mentioned method 4.; The second, also inoculating by above-mentioned method 4. as seed through the described quality pit mud diffusion juice 3. of 80 ℃ of processing in 10 minutes, it is for subsequent use that the seed in first, second described thin neck test tube is cultivated 7 days acquisition first order seeds respectively at 35-37 ℃;
6., secondary seed enlarges inoculation culture: use 150 ml flasks, with the synthetic nutrient solution of 150 ml flasks, plug, described caproic acid bacteria, cultivate ripe described 5. in first, second first order seed after the alcohol sterilization, put into sterilisable chamber, first order seed in described first, second thin neck test tube is accessed respectively in 150 ml flasks, add the synthetic nutrient solution of caproic acid bacteria to full bottle place, after shaking up with S type Glass tubing plug beyond the Great Wall, it is for subsequent use that the inherent 0.6Mpa/Hg of the glass evacuated moisture eliminator of packing into obtained secondary seed in 7 days in 35-37 ℃ of heat insulating culture;
7., three grades of seeds enlarge inoculation culture: use the long-neck Florence flask, with the synthetic nutrient solution of 1000 milliliters of long-neck Florence flasks, plug, described caproic acid bacteria, cultivate ripe described secondary seed 6. and after the alcohol sterilization, put into sterilisable chamber, described first, second secondary seed is mixed in the described long-neck Florence flask of 1000 milliliters of accesses, add the synthetic nutrient solution of described caproic acid bacteria to bottle everywhere, after plug shook up beyond the Great Wall, it was for subsequent use to obtain three grades of seeds in 7 days in 35-37 ℃ of heat insulating culture;
8., the level Four seed enlarges inoculation culture: use the Ka Shi tank, add dipotassium hydrogen phosphate 60 grams that dissolved, sodium acetate 60 grams, sal epsom 30 grams in ripe described 9 kilograms of yeast autolysis nutrient solutions 2. with cultivating, accessing in described three grades of seeds 7. behind the described quality pit mud diffusion juice 1L 3. that processed in 10 minutes through 80 ℃, put into two bottles of described Ka Shi tanks, add described yeast autolysis nutrient solution to tank everywhere, after shaking up with S type Glass tubing plug beyond the Great Wall, it is for subsequent use to obtain the level Four seed in 7 days in 35-37 ℃ of heat insulating culture;
9., the Pyatyi seed enlarges inoculation culture: use vat, dipotassium hydrogen phosphate 700 grams, the sodium acetate 700 that interpolation has been dissolved in ripe described 90 kilograms of yeast autolysis nutrient solutions 2. restrains, sal epsom 350 restrains with cultivating, 1 kilogram of quality pit mud, 9 kilograms in wheat koji powder through heating and keeping 10 minutes to 90 ℃, put into described vat, access again described level Four seed 8., add described yeast autolysis nutrient solution to cylinder everywhere, beat after consumption shakes up, with the plastic cloth sealing, it is for subsequent use to obtain the Pyatyi seed in 9 days in 35-37 ℃ of heat insulating culture;
10., close mud and cultivate the novel cellar for storing things mud of aromatic Chinese spirit: closing mud the day before yesterday will be closing the mud place, the pit mud culture pond, close the mud required equipment, the required starting material of the novel pit mud culture of instrument and aromatic Chinese spirit are prepared to put in place, close the mud batching by 40 kilograms of clays, 60 kilograms in mud, 10 kilograms of fresh vinasses, 10 kilograms of wheat kojis, 1 kilogram of soybean cake powder, 1 kilogram of quality pit mud, 0.2 kilogram of dipotassium hydrogen phosphate, 0.1 kilogram in sal epsom, 0.2 kilogram of sodium acetate, 10 kilograms of fresh yellow waters, 4 kilograms of 30 degree liquor, 10 kilograms of compositions of described Pyatyi seed 9., the above-mentioned mud batching of closing is drawn in and closed mud field boundary ground, after angling spade to turn evenly with three teeth, application is closed the mud machine and is squeezed in the described pit mud culture pond, be incubated 35-37 ℃ and cultivated 30 days, be prepared into the novel cellar for storing things mud of aromatic Chinese spirit.
2. Novel culture method of Luzhou-flavor liquor pit mud according to claim 1, it is characterized in that: the active dry yeast that has activated described in the described yeast autolysis nutrient solution is that 2% syrup of active dry yeast and 10 times of active dry yeast weight activates 3-4 hour under 30-32 ℃ of temperature.
3. described Novel culture method of Luzhou-flavor liquor pit mud according to claim 1 is characterized in that: described 1. to 10. preparation quantity be basic quantity, its actual quantity is determined for increasing in proportion according to actual needs on the basis of basic quantity.
4. described Novel culture method of Luzhou-flavor liquor pit mud according to claim 1 is characterized in that: the novel cellar for storing things mash of described aromatic Chinese spirit is put on the fermentation vat wall of aromatic Chinese spirit and ground at the end, for the production fermentation of aromatic Chinese spirit ready.
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| CN104694324B (en) * | 2014-03-05 | 2017-04-05 | 江苏乾天酒业有限公司 | A kind of pit mud preparation technology |
| CN106085741B (en) * | 2016-08-30 | 2019-05-21 | 花冠集团酿酒股份有限公司 | A kind of Luzhou-flavor liquo band pit mud nutrient fluid cultural method |
| CN107034108B (en) * | 2017-05-09 | 2020-07-07 | 江南大学 | Method for improving refreshing purity of Luzhou-flavor liquor through pit mud maintenance |
| CN109762692A (en) * | 2019-01-21 | 2019-05-17 | 四川大学 | A kind of method that brewery quickly adjusts pit mud nutrient fluid pH value |
| CN111019832B (en) * | 2019-12-30 | 2022-04-15 | 湖北工业大学 | Normal temperature preservation method of caproic acid bacteria |
| CN111440838A (en) * | 2020-03-25 | 2020-07-24 | 北京科技大学 | Method for producing hexanoic acid by fermenting white spirit vinasse by enriching hexanoic acid functional bacteria in pit mud |
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| CN1193090C (en) * | 1999-11-12 | 2005-03-16 | 四川沱牌曲酒股份有限公司 | Artificial distiller's yeast culturing process |
| CN101503654A (en) * | 2009-01-21 | 2009-08-12 | 江苏双沟酒业股份有限公司 | Aromatic Qu liquor pit mud and preparation thereof |
| CN101503655B (en) * | 2009-03-17 | 2012-05-23 | 李家民 | Preparation of artificial pit mud for improving aged aroma of aroma type white spirit |
| CN101768536B (en) * | 2010-02-11 | 2012-04-25 | 蒋钟璆 | Novel technique for quickly preparing aged cellar mud |
| CN101948722A (en) * | 2010-09-03 | 2011-01-19 | 刘怀颖 | Luzhou-flavor Daqu liquor pit mud and preparation method thereof |
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