CN105558897A - Manufacturing method of smelly mandarin fish - Google Patents

Manufacturing method of smelly mandarin fish Download PDF

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Publication number
CN105558897A
CN105558897A CN201610007558.1A CN201610007558A CN105558897A CN 105558897 A CN105558897 A CN 105558897A CN 201610007558 A CN201610007558 A CN 201610007558A CN 105558897 A CN105558897 A CN 105558897A
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China
Prior art keywords
mandarin fish
nutrient solution
hours
mucor racemosus
water
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CN201610007558.1A
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Chinese (zh)
Inventor
吕庆茂
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Harbin Weiping Technology Development Co Ltd
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Harbin Weiping Technology Development Co Ltd
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Priority to CN201610007558.1A priority Critical patent/CN105558897A/en
Publication of CN105558897A publication Critical patent/CN105558897A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum

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  • Meat, Egg Or Seafood Products (AREA)

Abstract

The invention belongs to the technical field of food processing, and especially relates to a manufacturing method of smelly mandarin fish. The manufacturing method comprises the following steps: washing a fresh mandarin fish, inoculating mucor racemosus onto the mandarin fish, carrying out fermentation for 30 to 40 hours, saving the mandarin fish for later use; adding laurel leaves, ginger slices, anise, Chinese prickly ash, salt, and white granulated sugar into clean water, heating the clean water until the water is boiling, cooling, adding liquor, a culture solution of staphylococcus epidermidis, and a culture solution of lactobacillus plantarum, evenly stirring to obtain a mixed bacterium fermented liquid; putting the fermented mandarin fish into a wood barrel, pouring the mixed bacterium fermented liquid into the wood barrel so as to submerge the mandarin fish, covering the wood barrel, carrying out fermentation for 15 to 20 hours at a temperature of 30 to 37 DEG C, then fermenting the mandarin fish for 1 to 2 days at a temperature of 15 to 20 DEG C, taking out the mandarin fish, freezing the mandarin fish, and packaging the mandarin fish to obtain finished product. The smelly mandarin fish manufactured by the provided method has the advantages of good taste, quick fermentation speed, stable quality and suitability for industrial production.

Description

The preparation method of a kind of smelly mandarin fish
Technical field
The invention belongs to food processing technology field, particularly the preparation method of a kind of smelly mandarin fish.
Background technology
Smelly mandarin fish, also known as smelly mandarin fish, is time-honored traditional famous dish together of China, is one of representative of Huizhou City dish, originates from Huizhou Area, Anhui (Huangshan, modern Anhui Province one is with).Smelly mandarin fish smells smelly, perfume (or spice) of tasting, the smooth mouth of Fresh & Tender in Texture, alcohol, maintains this taste Normal juice of mandarin fish.It is that the sulfur-containing amino acid in fish protein is hydrolyzed under the effect of the protease of microorganism secretion that smelly mandarin fish smells smelly reason, create hydrogen sulfide, hydrogen sulfide has rotten egg smell, and smelly mandarin fish fragrant reason of tasting is, after fish meat protein is decomposed by the microorganisms, produce amino acid, and amino acid has delicious flavour.Traditional smelly mandarin fish preparation method be pickled for fresh mandarin fish light salt brine in the environment of room temperature about 25 DEG C, push down with stone, time after tens days, fish body sends like after smelly non-foul smell taste, owing to being employing spontaneous fermentation, so fermentation period is longer, generally all at 10 ~ 20 days, easily there is the phenomenon of bacteria infection or unstable quality in sweat, be difficult to realize large-scale industrial production.
Summary of the invention
The object of the invention is the deficiency for the smelly mandarin fish way of tradition and the preparation method of a kind of features good taste provided, fast, the stay-in-grade smelly mandarin fish of fermenting speed.
The object of the present invention is achieved like this:
Take analysis for soybean powder 3 grams, maltose 0.5 gram, potassium dihydrogen phosphate 0.2 gram, 0.1 gram, magnesium sulfate, yeast extract 0.1 gram, add water and stir and be settled to 100 milliliters, pH is adjusted to 6.0 ~ 6.5,121 DEG C of sterilizings 20 minutes, be cooled to 28 ~ 32 DEG C, inoculation Mucor racemosus, 28 ~ 32 DEG C, cultivate 20 ~ 24 hours to obtain Mucor racemosus nutrient solution under the condition of rotary shaker 160 ~ 200rpm, Mucor racemosus nutrient solution and sterilized water are mixed and the Mucor racemosus inoculation liquid that stirs to obtain according to the ratio of mass ratio 1:50.
Choose fresh mandarin fish to clean, put in Mucor racemosus inoculation liquid to soak after 1 minute and take out, be placed in square position, ferment 30 ~ 40 hours in the insulating box of temperature 28 ~ 32 DEG C, period turned over once face every 10 hours.
Take peptone 1g, beef extract 1g, dusty yeast 0.5g, glucose 2g, magnesium sulfate 0.05g, sodium acetate 0.5g, Triammonium citrate 0.2g, dipotassium hydrogen phosphate 0.1g, manganese sulfate 0.05g, sodium chloride 5g, the stirring and dissolving that adds water also is settled to 100 milliliters, and pH is adjusted to 6.0 ~ 6.5,121 DEG C of sterilizings 20 minutes, be cooled to 28 ~ 32 DEG C, inoculation MRSE, 28 ~ 32 DEG C, cultivate 20 ~ 24 hours to obtain MRSE nutrient solution under the condition of rotary shaker 160 ~ 200rpm.
Take casein peptone 1g, beef extract 1g, dusty yeast 0.5g, glucose 0.5g, sodium acetate 0.5g, dibasic ammonium citrate 0.2g, potassium dihydrogen phosphate 0.1g, magnesium sulfate 0.05g, the stirring and dissolving that adds water also is settled to 100 milliliters, and pH is adjusted to 6.5 ~ 7.0,121 DEG C of sterilizings 20 minutes, be cooled to 35 ~ 38 DEG C, inoculation Lactobacillus plantarum, under 35 ~ 38 DEG C of conditions, static gas wave refrigerator obtains Lactobacillus plantarum nutrient solution in 20 ~ 24 hours.
0.5 ~ 1% basyleave is put in clear water, the ginger splices of 1 ~ 2%, the anise of 1 ~ 2%, the Chinese prickly ash of 1 ~ 2%, the salt of 4 ~ 5%, the white granulated sugar of 0.5 ~ 1%, then 1 ~ 2 minute is boiled, after being cooled to 30 ~ 40 DEG C, add 50 ~ 60 degree of white wine of the water yield 2 ~ 4%, then add MRSE nutrient solution and Lactobacillus plantarum nutrient solution, the addition of MRSE nutrient solution is 0.1 ~ 0.2% of the water yield, the addition of Lactobacillus plantarum nutrient solution is 0.3 ~ 0.5% of the water yield, stirs and obtains mixed cell zymotic fluid.
By the mandarin fish of fermenting through Mucor racemosus, be put in wooden barrel, mixed cell zymotic fluid is poured in wooden barrel, mandarin fish is flooded, covers bung, 30 ~ 37 DEG C of condition bottom fermentations 15 ~ 20 hours, ferment again under proceeding to 15 ~ 20 DEG C of conditions 1 ~ 2 day, get product through freezing, packaging after taking-up.
Utilize the smelly mandarin fish that method of the present invention makes, features good taste, fermenting speed be fast, batch between steady quality, be suitable for large-scale industrial production.
Specific embodiments
Mucor racemosus CICC40491, MRSE CICC10469 that the present invention adopts, Lactobacillus plantarum CICC21805 purchase in Chinese industrial Microbiological Culture Collection administrative center.
Take analysis for soybean powder 3 grams, maltose 0.5 gram, potassium dihydrogen phosphate 0.2 gram, 0.1 gram, magnesium sulfate, yeast extract 0.1 gram, add water and stir and be settled to 100 milliliters, pH is adjusted to 6.2,121 DEG C of sterilizings 20 minutes, be cooled to 30 DEG C, inoculation Mucor racemosus, 30 DEG C, cultivate 22 hours to obtain Mucor racemosus nutrient solution under the condition of rotary shaker 180rpm, Mucor racemosus nutrient solution and sterilized water are mixed and the Mucor racemosus inoculation liquid that stirs to obtain according to the ratio of mass ratio 1:50.
Choose fresh mandarin fish to clean, put in Mucor racemosus inoculation liquid to soak after 1 minute and take out, be placed in square position, ferment 35 hours in the insulating box of temperature 30 DEG C, period turned over once face every 10 hours.
Take peptone 1g, beef extract 1g, dusty yeast 0.5g, glucose 2g, magnesium sulfate 0.05g, sodium acetate 0.5g, Triammonium citrate 0.2g, dipotassium hydrogen phosphate 0.1g, manganese sulfate 0.05g, sodium chloride 5g, the stirring and dissolving that adds water also is settled to 100 milliliters, and pH is adjusted to 6.2,121 DEG C of sterilizings 20 minutes, be cooled to 30 DEG C, inoculation MRSE, 30 DEG C, cultivate 22 hours to obtain MRSE nutrient solution under the condition of rotary shaker 180rpm.
Take casein peptone 1g, beef extract 1g, dusty yeast 0.5g, glucose 0.5g, sodium acetate 0.5g, dibasic ammonium citrate 0.2g, potassium dihydrogen phosphate 0.1g, magnesium sulfate 0.05g, the stirring and dissolving that adds water also is settled to 100 milliliters, and pH is adjusted to 6.8,121 DEG C of sterilizings 20 minutes, be cooled to 37 DEG C, inoculation Lactobacillus plantarum, under 37 DEG C of conditions, static gas wave refrigerator obtains Lactobacillus plantarum nutrient solution in 22 hours.
0.5% basyleave is put into, the ginger splices of 2%, the anise of 1% in clear water, the Chinese prickly ash of 1%, the salt of 4.5%, the white granulated sugar of 0.5%, then boils 1 ~ 2 minute, after being cooled to 35 DEG C, add 50 degree of white wine of the water yield 4%, then add MRSE nutrient solution and Lactobacillus plantarum nutrient solution, the addition of MRSE nutrient solution is 0.15% of the water yield, the addition of Lactobacillus plantarum nutrient solution is 0.4% of the water yield, stirs and obtains mixed cell zymotic fluid.
By the mandarin fish of fermenting through Mucor racemosus, be put in wooden barrel, mixed cell zymotic fluid poured in wooden barrel, mandarin fish is flooded, cover bung, 34 DEG C of condition bottom fermentations 18 hours, ferment again under proceeding to 15 DEG C of conditions 2 days, get product through freezing, packaging after taking-up.

Claims (1)

1. a preparation method for smelly mandarin fish, is characterized in that being prepared from through following process:
(1) take analysis for soybean powder 3 grams, maltose 0.5 gram, potassium dihydrogen phosphate 0.2 gram, 0.1 gram, magnesium sulfate, yeast extract 0.1 gram, add water and stir and be settled to 100 milliliters, pH is adjusted to 6.0 ~ 6.5,121 DEG C of sterilizings 20 minutes, be cooled to 28 ~ 32 DEG C, inoculation Mucor racemosus, 28 ~ 32 DEG C, cultivate 20 ~ 24 hours to obtain Mucor racemosus nutrient solution under the condition of rotary shaker 160 ~ 200rpm, Mucor racemosus nutrient solution and sterilized water are mixed and the Mucor racemosus inoculation liquid that stirs to obtain according to the ratio of mass ratio 1:50;
(2) choose fresh mandarin fish to clean, put in Mucor racemosus inoculation liquid to soak after 1 minute and take out, be placed in square position, ferment 30 ~ 40 hours in the insulating box of temperature 28 ~ 32 DEG C, period turned over once face every 10 hours;
(3) take peptone 1g, beef extract 1g, dusty yeast 0.5g, glucose 2g, magnesium sulfate 0.05g, sodium acetate 0.5g, Triammonium citrate 0.2g, dipotassium hydrogen phosphate 0.1g, manganese sulfate 0.05g, sodium chloride 5g, the stirring and dissolving that adds water also is settled to 100 milliliters, and pH is adjusted to 6.0 ~ 6.5,121 DEG C of sterilizings 20 minutes, be cooled to 28 ~ 32 DEG C, inoculation MRSE, 28 ~ 32 DEG C, cultivate 20 ~ 24 hours to obtain MRSE nutrient solution under the condition of rotary shaker 160 ~ 200rpm;
(4) take casein peptone 1g, beef extract 1g, dusty yeast 0.5g, glucose 0.5g, sodium acetate 0.5g, dibasic ammonium citrate 0.2g, potassium dihydrogen phosphate 0.1g, magnesium sulfate 0.05g, the stirring and dissolving that adds water also is settled to 100 milliliters, and pH is adjusted to 6.5 ~ 7.0,121 DEG C of sterilizings 20 minutes, be cooled to 35 ~ 38 DEG C, inoculation Lactobacillus plantarum, under 35 ~ 38 DEG C of conditions, static gas wave refrigerator obtains Lactobacillus plantarum nutrient solution in 20 ~ 24 hours;
(5) in clear water, put into 0.5 ~ 1% basyleave, the ginger splices of 1 ~ 2%, the anise of 1 ~ 2%, the Chinese prickly ash of 1 ~ 2%, the salt of 4 ~ 5%, the white granulated sugar of 0.5 ~ 1%, then 1 ~ 2 minute is boiled, after being cooled to 30 ~ 40 DEG C, add 50 ~ 60 degree of white wine of the water yield 2 ~ 4%, then add MRSE nutrient solution and Lactobacillus plantarum nutrient solution, the addition of MRSE nutrient solution is 0.1 ~ 0.2% of the water yield, the addition of Lactobacillus plantarum nutrient solution is 0.3 ~ 0.5% of the water yield, stirs and obtains mixed cell zymotic fluid;
(6) the mandarin fish of will ferment through Mucor racemosus, is put in wooden barrel, and mixed cell zymotic fluid is poured in wooden barrel, mandarin fish is flooded, covers bung, 30 ~ 37 DEG C of condition bottom fermentations 15 ~ 20 hours, ferment again under proceeding to 15 ~ 20 DEG C of conditions 1 ~ 2 day, get product through freezing, packaging after taking-up.
CN201610007558.1A 2016-01-07 2016-01-07 Manufacturing method of smelly mandarin fish Withdrawn CN105558897A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106072039A (en) * 2016-06-28 2016-11-09 安徽徽滋坊食品贸易有限公司 A kind of smelly Siniperca chuatsi manufacture method
CN106261964A (en) * 2016-08-16 2017-01-04 武汉市农业科学技术研究院水产科学研究所 A kind of production method of Hubei flavor fermentation Siniperca chuatsi
CN107410939A (en) * 2017-06-26 2017-12-01 安徽省农业科学院农产品加工研究所 A kind of preparation method for the fermentation mandarin fish for being used for steamed culinary art
CN109480221A (en) * 2018-12-22 2019-03-19 大连民族大学 It is a kind of by Mucor and the gadus of Fermentation Condition of Monascus spp and preparation method thereof
CN111411139A (en) * 2020-03-02 2020-07-14 安徽省农业科学院农产品加工研究所 Cysteine-containing peptide of mandarin fish protein source and preparation method thereof, cysteine-containing peptide-zinc chelate and preparation method and application thereof
CN111411052A (en) * 2020-03-02 2020-07-14 安徽省农业科学院农产品加工研究所 Staphylococcus saprophyticus and application thereof in preparation of smelly mandarin fish flavor material
CN112841565A (en) * 2021-02-05 2021-05-28 中国水产科学研究院南海水产研究所 Processing method for enhancing fish meat elasticity by biological method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1903046A (en) * 2005-07-30 2007-01-31 孙义国 Method for pickling mandarin fish
CN102871151A (en) * 2012-10-17 2013-01-16 安徽省农业科学院农产品加工研究所 Manufacturing method of mandarin fish product
CN104223198A (en) * 2014-09-30 2014-12-24 安徽省农业科学院农产品加工研究所 Marinate mandarin fish production method
CN104757608A (en) * 2015-05-05 2015-07-08 安徽省绩溪县劳模实业有限公司 Pickling method for Hui school smelly fish

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1903046A (en) * 2005-07-30 2007-01-31 孙义国 Method for pickling mandarin fish
CN102871151A (en) * 2012-10-17 2013-01-16 安徽省农业科学院农产品加工研究所 Manufacturing method of mandarin fish product
CN104223198A (en) * 2014-09-30 2014-12-24 安徽省农业科学院农产品加工研究所 Marinate mandarin fish production method
CN104757608A (en) * 2015-05-05 2015-07-08 安徽省绩溪县劳模实业有限公司 Pickling method for Hui school smelly fish

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106072039A (en) * 2016-06-28 2016-11-09 安徽徽滋坊食品贸易有限公司 A kind of smelly Siniperca chuatsi manufacture method
CN106261964A (en) * 2016-08-16 2017-01-04 武汉市农业科学技术研究院水产科学研究所 A kind of production method of Hubei flavor fermentation Siniperca chuatsi
CN107410939A (en) * 2017-06-26 2017-12-01 安徽省农业科学院农产品加工研究所 A kind of preparation method for the fermentation mandarin fish for being used for steamed culinary art
CN109480221A (en) * 2018-12-22 2019-03-19 大连民族大学 It is a kind of by Mucor and the gadus of Fermentation Condition of Monascus spp and preparation method thereof
CN111411139A (en) * 2020-03-02 2020-07-14 安徽省农业科学院农产品加工研究所 Cysteine-containing peptide of mandarin fish protein source and preparation method thereof, cysteine-containing peptide-zinc chelate and preparation method and application thereof
CN111411052A (en) * 2020-03-02 2020-07-14 安徽省农业科学院农产品加工研究所 Staphylococcus saprophyticus and application thereof in preparation of smelly mandarin fish flavor material
CN111411052B (en) * 2020-03-02 2022-04-08 安徽省农业科学院农产品加工研究所 Staphylococcus saprophyticus and application thereof in preparation of smelly mandarin fish flavor material
CN111411139B (en) * 2020-03-02 2022-04-29 安徽省农业科学院农产品加工研究所 Cysteine-containing peptide of mandarin fish protein source and preparation method thereof, cysteine-containing peptide-zinc chelate and preparation method and application thereof
CN112841565A (en) * 2021-02-05 2021-05-28 中国水产科学研究院南海水产研究所 Processing method for enhancing fish meat elasticity by biological method

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