CN101513161A - Formula for culture medium of cordyceps militaris liquid strains and method for culturing same - Google Patents
Formula for culture medium of cordyceps militaris liquid strains and method for culturing same Download PDFInfo
- Publication number
- CN101513161A CN101513161A CNA2009100262554A CN200910026255A CN101513161A CN 101513161 A CN101513161 A CN 101513161A CN A2009100262554 A CNA2009100262554 A CN A2009100262554A CN 200910026255 A CN200910026255 A CN 200910026255A CN 101513161 A CN101513161 A CN 101513161A
- Authority
- CN
- China
- Prior art keywords
- cordyceps militaris
- culture medium
- cultural method
- culture
- bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a formula for the culture medium of cordyceps militaris liquid strains and a method for culturing the same. The invention is characterized in that the method comprises the following steps: preparing a culture solution with the pH value being 7.5 from the following components by percentage: 0.8% of peptone, 2% of glucose, 0.1% of ammonium citrate, 0.1% of magnesium sulfate, 0.1% of monopotassium phosphate, 0.002% of vitamin B1 and 92.9% of water; filling containers, such as erlenmeyer flasks, saline bottles or preserving jars, with the culture solution; carrying out regular autoclaving on the culture solution; then, grafting the slant mother culture of cordyceps militaris onto the culture solution; and carrying out standing and culturing for 7 to 10 days in a light-blocking and airtight culture room with the temperature being 18 to 22 DEG C to obtain the cordyceps militaris liquid strains. The invention dispenses with fermentation tanks, shaking tables and other devices, therefore, the investment is small; no sterile air is introduced, therefore, the operation is convenient; and no energy is consumed, as a result, the invention is energy-saving and environment-friendly.
Description
Technical field
The present invention relates to a kind of Cordyceps militaris liquid spawn culture medium prescription and cultural method, belong to technical field of edible fungi production.
Background technology
Cordyceps militaris is also named Cordyceps militaris, is described as " east mountain balsam " by people, is a kind of precious Chinese herbal medicine with very high health care and nourishing value.The contained many medicinal ingredients of Cordyceps militaris all near or surpass Cordyceps sinensis, have with the similar magical drug effect of Cordyceps sinensis.After artificial cultivation Cordyceps militaris technology was achieved success, development speed was very fast in recent years, and Cordyceps militaris output increases sharply, and price reduces significantly, and apparent consumption also increases thereupon, had from the trend of medicine to the food development.2008, it was new resource food that the Ministry of Public Health has ratified Cordyceps militaris, will bring bright prospects for the Cordyceps militaris industry development.At present, artificial cultivation Cordyceps militaris, great majority adopt Cans as cultivating container, as main medium matter, required bacterial classification 3-4 days must cover with charge level, otherwise medium will go bad rotten with rice, cause the cultivation failure.Conventional food (medicine) can not be suitable for starter kind, original seed, production kind three tier structure kind method.The tool cultivation practices, the bacterial classification of Cordyceps militaris preferably adopts liquid spawn, could be easy to use, guarantee inoculation evenly, fast growth.In the prior art, food (medicine) is used the bacteria liquid bacterium culture medium, mainly filters with material cookings such as potato, analysis for soybean powder, corn flour, wheat brans, adds some glucose and trace element, adds water again and mixes.This medium is relatively more muddy, and transparency is not high, is difficult to observe the mycelial growth situation, is difficult for differentiating the bacterial classification quality, and the situation that the pollution bacterial classification is used to produce happens occasionally.Usually based on ventilation or stir culture, generally use equipment such as fermentation tank, shaking table on the cultural method, output investment ratio is bigger, operate more loaded down with trivial details, incompatibility ordinary people family production.
Summary of the invention
In order to overcome existing liquid spawn culture medium muddiness, transparency is not high, be difficult to observe the mycelial growth situation and use equipment such as fermentation tank, shaking table, investment is big, complex operation, deficiencies such as incompatibility ordinary people family production, the present invention discloses a kind of Cordyceps militaris liquid spawn culture medium prescription and cultural method, this medium not only is fit to the scarlet caterpiller fungus mycelium growth, and as clear as crystal, be convenient to observe the mycelial growth situation, can in time find and handle the bacterial classification of pollution, reduce because bacterial classification pollutes the unnecessary loss that inoculation causes, improve the cordyceps militaris cultivation success rate.This cultural method does not use equipment such as fermentation tank, shaking table, need not ventilate or stir culture, can successfully turn out energetic, the easy to use Cordyceps militaris liquid spawn of mycelia stalwartness.
Technical scheme of the present invention is achieved in that
1, culture medium prescription: peptone 0.8%, glucose 2%, ammonium citrate 0.1%, magnesium sulfate 0.1%, potassium dihydrogen phosphate 0.1%, Cobastab
10.002%, water 92.9%, pH value 7.5.
2, compound method: prepare quantity as required and take by weighing above-mentioned formula material in proportion respectively, earlier with Cobastab
1Be dissolved in water after pulverizing,, then peptone dissolved aqueous solution back and other raw materials with hot water, after 6-8 layer filtered through gauze, add water and complement to preparation quantity again with glucose, ammonium citrate, magnesium sulfate, the dissolving of potassium dihydrogen phosphate water.Adjust pH value to 7.5 with sodium hydrate aqueous solution at last.
3, bottling sterilization: the culture fluid for preparing is packed in 500 milliliters triangular flask, saline bottle or the Cans by the quantity of every bottle of 200-250 milliliter, after sealing with cotton or polypropylene film, putting into medical portable pressure cooker sterilizes, 1.1-1.3 kg/cm pressure kept 30-35 minute.
4, inoculation: after treating that culture-liquid temp is cooled to below 30 ℃, every bottle graft is gone into flush Cordyceps militaris inclined-plane female 2-3 of kind piece of grain of rice size of mycelia in gnotobasis.
5, cultural method: the bottle that will connect bacterial classification is put into the spawn culture chamber and carries out the airtight cultivation of shading, leaves standstill earlier and cultivates 24 hours, and manually rock 1-2 time later every day, prevents clumping of over grown hyphae, checks the mycelial growth situation in passing, finds that suspicious bacterial classification in time rejects.Culturing room's temperature is controlled at 18-22 ℃, and humidity keeps 70-80%, just can obtain the Cordyceps militaris liquid spawn in 7-10 days.Beneficial effect of the present invention: compared with prior art, the present invention has the following advantages:
1, all raw materials of medium all can directly be dissolved in water, do not need boiling, and are easy to make.
2, sterilization back culture fluid is as clear as crystal, is convenient to observe the mycelial growth situation, can in time find and handle the bacterial classification of pollution, reduces because bacterial classification pollutes the unnecessary loss that inoculation causes, and improves the cordyceps militaris cultivation success rate.
3, this cultural method does not use equipment such as fermentation tank, shaking table, does not need ventilation or stir culture, can save investment greatly, reduces production costs, and is fit to very much the ordinary people and applies.
4, this not electricity consumption of cultural method, consumes energy not, noiselessness without any pollution, meets the industrial policy of national energy-saving and environmental protecting to environment.
5, this method need not to feed filtrated air, and the enough mycelial growths of air need in the bottle, and the air inlet that can avoid like this causing because of the filter effect is bad is polluted, and improves the bacterial classification yield rate.
Embodiment:
Embodiment 1
1, prepare 14 of clean 500ml Cans, length of side 12cm, square polypropylene film and rubber band that thickness is 5 are an amount of.
2, preparation medium: earlier with Cobastab
16 (60 milligrams), be dissolved in water after pulverizing, take by weighing glucose 60 grams again, ammonium citrate 3 grams, magnesium sulfate 3 restrains, potassium dihydrogen phosphate 3 gram waters dissolvings, peptone 24 grams are dissolved back and other raw materials with hot water aqueous solution then, after 6-8 layer filtered through gauze, add water and complement to 3000 milliliters, adjust pH value to 7.5 with sodium hydrate aqueous solution at last.
3, bottling sterilization: the culture fluid for preparing is packed in the Cans by the amount of every bottle of 200-250 milliliter, after sealing with ready polypropylene film and rubber band, put into medical portable pressure cooker sterilization (can only adorn 14 of Cans), autoclaving operation sequence routinely, 1.1-1.3 kg/cm pressure kept 30-35 minute.
4, inoculation: after treating that culture-liquid temp is cooled to below 30 ℃, every bottle graft is gone into flush Cordyceps militaris inclined-plane female 2-3 of kind piece of grain of rice size of mycelia in gnotobasis.
5, cultivate: the Cans that will connect bacterial classification are put into culturing room and carry out the airtight cultivation of shading, leave standstill earlier to cultivate 24 hours, and manually rock 1-2 time later every day, prevents clumping of over grown hyphae, checks the mycelial growth situation in passing, find that suspicious bacterial classification in time rejects.Culturing room's temperature is controlled at 18-22 ℃, and humidity keeps 70-80%, just can obtain the Cordyceps militaris liquid spawn that can be used to produce in 7-10 days.
6, inoculation is handled: with cultured Cordyceps militaris liquid spawn, under gnotobasis, pick up the mycoderma that lumps with aseptic tweezers, add the 200ml sterile water, stir bacterium liquid again, smash mycelia, guarantee even inoculation, reduce and stop up.
7, use: the Cordyceps militaris liquid spawn of handling well is poured into through in the inoculation watering can of aseptic process, evenly be sprayed onto successively on the Cordyceps militaris rice medium of the bacterium of going out and get final product every bottle of sprinkling amount 3-5ml.
Embodiment 2
1,500ml triangular flask or saline bottle are cleaned.According to the bacterial classification requirement, determine to produce the bottle number, determine culture fluid quantity, every bottle of 200-250ml according to producing the bottle number again.
2, preparation medium: add peptone 8 grams by 1000 milliliters of culture fluids, glucose 20 grams, ammonium citrate 1 gram, magnesium sulfate 1 gram, potassium dihydrogen phosphate 1 gram, Cobastab
120 milligrams ratio takes by weighing needed raw material.Earlier with Cobastab
1Be dissolved in water after pulverizing, again with glucose, ammonium citrate, magnesium sulfate, the dissolving of potassium dihydrogen phosphate water, then peptone is dissolved the aqueous solution of back and other raw materials with hot water, after 6-8 layer filtered through gauze, add water and complement to the culture fluid quantity that needs are produced, adjust pH value to 7.5 with sodium hydrate aqueous solution at last.
3, bottling sterilization: with the culture fluid for preparing by the quantity of every bottle of 200-250 milliliter pack into triangular flask or saline bottle, cotton plug beyond the Great Wall, wrap brown paper, putting into medical portable pressure cooker sterilizes, autoclaving operation sequence routinely, 1.1-1.3 kg/cm pressure kept 30-35 minute.
4, inoculation: after treating that culture-liquid temp is cooled to below 30 ℃, every bottle graft is gone into flush Cordyceps militaris inclined-plane female 2-3 of kind piece of grain of rice size of mycelia in gnotobasis.
5, cultivate: the Cans that will connect bacterial classification are put into culturing room and carry out the airtight cultivation of shading, leave standstill earlier to cultivate 24 hours, and manually rock 1-2 time later every day, prevents clumping of over grown hyphae, checks the mycelial growth situation in passing, find that suspicious bacterial classification in time rejects.Culturing room's temperature is controlled at 18-22 ℃, and humidity keeps 70-80%, just can obtain the Cordyceps militaris liquid spawn that can be used to produce in 7-10 days.
6, inoculation is handled: with cultured Cordyceps militaris liquid spawn, under gnotobasis, pick up the mycoderma that lumps with aseptic tweezers, add the 200ml sterile water, stir bacterium liquid again, smash mycelia, guarantee even inoculation, reduce and stop up.
7, use: the Cordyceps militaris liquid spawn of handling well is poured into through in the inoculation watering can of aseptic process, evenly be sprayed onto successively on the Cordyceps militaris rice medium of the bacterium of going out and get final product every bottle of sprinkling amount 3-5ml.
Claims (6)
1. a Cordyceps militaris liquid spawn culture medium is filled a prescription and cultural method, it is characterized in that:
1) culture medium prescription: peptone 0.8%, glucose 2%, ammonium citrate 0.1%, magnesium sulfate 0.1%, potassium dihydrogen phosphate 0.1%, Cobastab
10.002%, water 92.9%, pH value 7.5.
2) compound method: prepare quantity as required and take by weighing above-mentioned formula material in proportion respectively, earlier with Cobastab
1Be dissolved in water after pulverizing,, then peptone dissolved aqueous solution back and other raw materials with hot water, after 6-8 layer filtered through gauze, add water and complement to preparation quantity again with glucose, ammonium citrate, magnesium sulfate, the dissolving of potassium dihydrogen phosphate water.Adjust pH value to 7.5 with sodium hydrate aqueous solution at last.
3) bottling sterilization: the culture fluid for preparing is packed in the culture vessel bottle, after sealing, put into medical portable pressure cooker and sterilize.
4) inoculation: after treating that culture-liquid temp is cooled to below 30 ℃, insert the female kind in Cordyceps militaris inclined-plane.
5) cultural method: the bottle that will connect bacterial classification is put into the spawn culture chamber and carries out the airtight cultivation of shading, just can obtain the Cordyceps militaris liquid spawn that can be used to produce in 7-10 days.
2, according to described a kind of Cordyceps militaris liquid spawn culture medium prescription of claim 1 and cultural method, it is characterized in that: described culture vessel bottle can be triangular flask, saline bottle or Cans etc., volume 500ml.
3, a kind of Cordyceps militaris liquid spawn culture medium prescription according to claim 1 and cultural method is characterized in that: described sealing, and Cans adopt length of side 12cm, and the square polypropylene film that thickness is 5 is tied tight with rubber band; Triangular flask, saline bottle adopt cotton plug or polypropylene film to seal.
4, a kind of Cordyceps militaris liquid spawn culture medium prescription according to claim 1 and cultural method, it is characterized in that: described sterilization is an autoclaving operation sequence routinely, 1.1-1.3 kg/cm pressure kept 30-35 minute.
5, according to described a kind of Cordyceps militaris liquid spawn culture medium prescription of claim 1 and cultural method, it is characterized in that: described inoculation is to insert female kind the in Cordyceps militaris inclined-plane that mycelia flushes in gnotobasis, quantity 2-3 piece, and size is similar to the grain of rice.
6, a kind of Cordyceps militaris liquid spawn culture medium prescription according to claim 1 and cultural method, it is characterized in that: the airtight cultivation of described shading, be that the seed bottle that will inoculate leaves standstill cultivation 24 hours earlier, manually rock 1-2 time every day later on, prevent clumping of over grown hyphae, check the mycelial growth situation in passing, find that suspicious bacterial classification in time rejects.Culturing room's temperature is controlled at 18-22 ℃, and humidity keeps 70-80%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100262554A CN101513161A (en) | 2009-03-18 | 2009-03-18 | Formula for culture medium of cordyceps militaris liquid strains and method for culturing same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100262554A CN101513161A (en) | 2009-03-18 | 2009-03-18 | Formula for culture medium of cordyceps militaris liquid strains and method for culturing same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101513161A true CN101513161A (en) | 2009-08-26 |
Family
ID=41037908
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2009100262554A Pending CN101513161A (en) | 2009-03-18 | 2009-03-18 | Formula for culture medium of cordyceps militaris liquid strains and method for culturing same |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101513161A (en) |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102084780A (en) * | 2010-05-31 | 2011-06-08 | 陈卫东 | Method for culturing cordyceps militaris sporocarp with high-content bioactive substances |
| CN102229507A (en) * | 2011-05-28 | 2011-11-02 | 镇江市食用菌研究所 | Cordyceps militaris water culturing medium |
| CN102293122A (en) * | 2011-06-24 | 2011-12-28 | 王满玉 | Cultivating method for cordyceps militaris by using manyprickle acathopanax root |
| CN102318544A (en) * | 2011-05-28 | 2012-01-18 | 镇江市食用菌研究所 | A kind of Cordyceps militaris ciltivating process |
| CN102342217A (en) * | 2010-07-29 | 2012-02-08 | 于兴军 | Pollution-free cordyceps militaris strain production or fermentation method |
| CN102696402A (en) * | 2012-07-04 | 2012-10-03 | 黑龙江大学 | Culturing method of liquorice-based cordyceps militaris |
| CN102715012A (en) * | 2012-06-13 | 2012-10-10 | 镇江市食用菌研究所 | Cultivation method of Cordyceps militaris |
| CN104163685A (en) * | 2014-06-30 | 2014-11-26 | 桂林丰茂源农业技术开发有限公司 | A cordyceps militaris sporophore culture medium comprising Chinese globeflower broken bits |
| CN104585510A (en) * | 2015-02-13 | 2015-05-06 | 秦皇岛高通生物科技有限公司 | Feed additive containing mixed bacterium fermented product and preparation method for feed additive |
| CN104584855A (en) * | 2013-10-31 | 2015-05-06 | 江苏学府生物工程有限公司 | Cordyceps militaris extruding, puffing and sterilizing production method |
| CN104920064A (en) * | 2015-05-20 | 2015-09-23 | 湖州师范学院 | Preparing method of mother culture special for production of Cordyceps militaris |
| CN105660187A (en) * | 2016-02-23 | 2016-06-15 | 张家港顺泰元生物科技有限公司 | Aseptic silkworm cordyceps militaris strain inoculation system and method |
| CN107347446A (en) * | 2017-07-25 | 2017-11-17 | 南江宏信生物科技有限公司 | The cultural method of Cordceps militaris |
| CN107960270A (en) * | 2017-08-31 | 2018-04-27 | 广西沙田仙人滩农业投资有限公司 | A kind of method of cordyceps militaris cultivation |
| CN108633627A (en) * | 2018-08-13 | 2018-10-12 | 梅州市佳诚食用菌有限公司 | The method of cultured mycelia |
-
2009
- 2009-03-18 CN CNA2009100262554A patent/CN101513161A/en active Pending
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102084780B (en) * | 2010-05-31 | 2013-02-13 | 陈卫东 | Method for culturing cordyceps militaris sporocarp with high-content bioactive substances |
| CN102084780A (en) * | 2010-05-31 | 2011-06-08 | 陈卫东 | Method for culturing cordyceps militaris sporocarp with high-content bioactive substances |
| CN102342217A (en) * | 2010-07-29 | 2012-02-08 | 于兴军 | Pollution-free cordyceps militaris strain production or fermentation method |
| CN102229507A (en) * | 2011-05-28 | 2011-11-02 | 镇江市食用菌研究所 | Cordyceps militaris water culturing medium |
| CN102318544A (en) * | 2011-05-28 | 2012-01-18 | 镇江市食用菌研究所 | A kind of Cordyceps militaris ciltivating process |
| CN102229507B (en) * | 2011-05-28 | 2013-07-03 | 镇江市食用菌研究所 | Cordyceps militaris water culturing medium |
| CN102293122A (en) * | 2011-06-24 | 2011-12-28 | 王满玉 | Cultivating method for cordyceps militaris by using manyprickle acathopanax root |
| CN102293122B (en) * | 2011-06-24 | 2012-07-25 | 王满玉 | Cultivating method for cordyceps militaris by using manyprickle acathopanax root |
| CN102715012A (en) * | 2012-06-13 | 2012-10-10 | 镇江市食用菌研究所 | Cultivation method of Cordyceps militaris |
| CN102696402A (en) * | 2012-07-04 | 2012-10-03 | 黑龙江大学 | Culturing method of liquorice-based cordyceps militaris |
| CN104584855A (en) * | 2013-10-31 | 2015-05-06 | 江苏学府生物工程有限公司 | Cordyceps militaris extruding, puffing and sterilizing production method |
| CN104163685A (en) * | 2014-06-30 | 2014-11-26 | 桂林丰茂源农业技术开发有限公司 | A cordyceps militaris sporophore culture medium comprising Chinese globeflower broken bits |
| CN104163685B (en) * | 2014-06-30 | 2016-08-24 | 桂林丰茂源农业技术开发有限公司 | A kind of Cordyceps militaris (L.) Link.sporophore culture medium adding Flos Trollii slag |
| CN104585510A (en) * | 2015-02-13 | 2015-05-06 | 秦皇岛高通生物科技有限公司 | Feed additive containing mixed bacterium fermented product and preparation method for feed additive |
| CN104585510B (en) * | 2015-02-13 | 2017-11-24 | 秦皇岛高通生物科技有限公司 | A kind of mixed fungus fermentation thing feed addictive and preparation method thereof |
| CN104920064A (en) * | 2015-05-20 | 2015-09-23 | 湖州师范学院 | Preparing method of mother culture special for production of Cordyceps militaris |
| CN105660187A (en) * | 2016-02-23 | 2016-06-15 | 张家港顺泰元生物科技有限公司 | Aseptic silkworm cordyceps militaris strain inoculation system and method |
| CN107347446A (en) * | 2017-07-25 | 2017-11-17 | 南江宏信生物科技有限公司 | The cultural method of Cordceps militaris |
| CN107960270A (en) * | 2017-08-31 | 2018-04-27 | 广西沙田仙人滩农业投资有限公司 | A kind of method of cordyceps militaris cultivation |
| CN108633627A (en) * | 2018-08-13 | 2018-10-12 | 梅州市佳诚食用菌有限公司 | The method of cultured mycelia |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101513161A (en) | Formula for culture medium of cordyceps militaris liquid strains and method for culturing same | |
| CN104012298B (en) | The complete production of hybrid seeds technique of edible fungus liquid kind submerged fermentation and culture medium prescription thereof | |
| CN102523917B (en) | Method for cultivating straw mushroom | |
| CN102057836B (en) | Method for quickly producing edible fungus liquid strain by utilizing primary-secondary type culture tank | |
| CN102835251B (en) | Submerged fermentation culturing method for medicinal hericium erinaceus mycelium liquid | |
| CN108522153A (en) | A method of utilizing liquid spawn fast-propagation hickory chick cultigen | |
| CN107488598B (en) | Burdock-based cordyceps militaris mycelium and preparation method thereof | |
| CN104431090A (en) | Tea fungus production method and tea fungus product | |
| CN102845225A (en) | Hypsizygus marmoreus liquid strain fermenting technique | |
| CN105309750A (en) | Comprehensive utilization and production technology of high-content antrodia camphorate fungal active polysaccharide, triterpene fine powder, and byproducts thereof | |
| CN108934785B (en) | Liquid strain culture method and cultivation method of boletus nigricans | |
| CN102224792A (en) | Aquaculture method of selenium-rich hericium erinaceus | |
| CN1837354A (en) | Ganoderma lucidum hypha powder or ganoderma lucidum tea and dual fermentation process thereof | |
| CN108718915A (en) | Improve the culture medium and cultural method of pleurotus edible fungus yield | |
| CN107384824A (en) | Stalk rotten agent soon | |
| CN102318545B (en) | Water culturing method for hericium erinaceus | |
| CN102318544A (en) | A kind of Cordyceps militaris ciltivating process | |
| CN106635829B (en) | A kind of preparation method of Phellinus granular bacteria strain | |
| CN102742690A (en) | Health-care functional tea and preparation method thereof | |
| CN107746810A (en) | A kind of method for effectively improving Phellinus liquid cultured mycelia yield | |
| CN100513549C (en) | Liquid mushroom producing method and fermenting set | |
| CN105602857B (en) | A kind of optimization method that wild cicada fungus liquid spawn is manually cultivated | |
| CN110150029A (en) | Application of the Chinese redbud in terms of edible fungus culturing | |
| CN103283487B (en) | Method for culturing pleurotus geesteranus liquid-state strains | |
| CN108179116A (en) | A kind of mushroom high density fermentation production method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: ZHENJIANG CITY MUSHROOM INSTITUTE Free format text: FORMER OWNER: ZHU ZHONGGUI Effective date: 20091023 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20091023 Address after: Institute of edible fungi in Jiangsu city of Zhenjiang Province Post encoding Zongze Road No. 118 Zhenjiang City: 212008 Applicant after: Zhenjiang City Edible Fungus Research Institute Address before: Institute of edible fungi in Jiangsu city of Zhenjiang Province Post encoding Zongze Road No. 118 Zhenjiang City: 212008 Applicant before: Zhu Zhonggui |
|
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20090826 |