CN102342217A - Pollution-free cordyceps militaris strain production or fermentation method - Google Patents

Abstract

The invention relates to a pollution-free cordyceps militaris strain production or fermentation method, which comprises the following steps that: water is filled into and adequately mixed with a biological agent which facilitates the fermentation of cordyceps militaris strain to form a biological agent solution, and then the biological agent solution is filtered for use; water is added into ingredients of culture medium to fix the volume, then the biological agent solution is added, and the constant-temperature culture is executed after the steps of high-pressure sterilization and aseptic inoculation. The pollution-free cordyceps militaris strain production or fermentation method has beneficial effects that: the addition of antibiotics such as mycillin can be avoided, so no antibiotic remains in a finished product.

Description

A kind of pollution-free Cordyceps militaris spawn production or fermentation process

Technical field

The present invention relates to the Cordyceps militaris artificial breeding technique.

Background technology

Like Fig. 1, shown in Figure 2, in the market in the production process of like product, in order to improve output and control microbial contamination, and adopt interpolation blue or green, streptomycin more, cause in the product inevitably residually, and then can produce harmful effect to long-term user.

Particularly:

The flow sequence of first kind of cultural method employing is successively: water constant volume, autoclaving, aseptic inoculation, constant temperature culture are prepared burden, added to medium.

The flow sequence of second kind of cultural method employing is successively: water constant volume, mycillin interpolation, autoclaving, aseptic inoculation, constant temperature culture are prepared burden, added to medium.

Promptly second kind of cultural method added blue or green, streptomycin on the basis of first kind of cultural method.

Summary of the invention

The purpose of this invention is to provide a kind of pollution-free Cordyceps militaris spawn production or fermentation process, solve the residual problem of interpolation, antibiotic of antibiotic such as mycillin.

The objective of the invention is to realize through following technical scheme:

A kind of pollution-free Cordyceps militaris spawn production or fermentation process comprise that step is following:

The biologic product that will help the Cordyceps militaris spawn fermentation adds water and mixes fully formation biological solutions, and the filtration back is subsequent use;

Medium batching adds the water constant volume, adds biological solutions afterwards, pass through autoclaving, aseptic inoculation step then after, carry out constant temperature culture.

Beneficial effect of the present invention is: can exempt the interpolation of antibiotic such as mycillin, no any antibiotic is residual in the finished product.

Description of drawings

According to accompanying drawing and embodiment the present invention is done further explain below.

Fig. 1 is the flow chart of first kind of cultural method described in the technical background;

Fig. 2 is the flow chart of second kind of cultural method described in the technical background;

Fig. 3 is the flow chart of pollution-free Cordyceps militaris spawn production of the present invention or fermentation process.

Embodiment

As shown in Figure 3, a kind of pollution-free Cordyceps militaris spawn production or fermentation process comprise that step is following:

The biologic product that will help the Cordyceps militaris spawn fermentation adds water and mixes fully formation biological solutions, and the filtration back is subsequent use;

Medium batching adds the water constant volume, adds biological solutions afterwards, pass through autoclaving, aseptic inoculation step then after, carry out constant temperature culture.

Beneficial effect of the present invention is: can exempt the interpolation of antibiotic such as mycillin, no any antibiotic is residual in the finished product.

(1) in production procedure, before the medium autoclaving is handled, increase this case and handle (original production flow process 1), or the mycillin of cancelling former flow process adds (original production flow process 2) and continues afterwards to be undertaken by conventional method.

(2) in Cordyceps militaris spawn production at present or the fermentation production process, there is microbiological contamination rate high (original production flow process 1), and handles, can reduce the microbiological contamination rate on the one hand, thereby increase output through this case.

(3) in Cordyceps militaris spawn production at present or the fermentation production process, also there is the problem of mycillin residual (original production flow process 2).The used technology of this law is eliminated artificial antibiotic interpolations such as mycillin, thus in the finished product fruiting bodies of cordyceps militaris artificial antibiotic residue such as no mycillin, thereby the raising product quality.

With respect to original production flow process 1, can improve Cordyceps militaris output through this case processing, improve the enterprise production benefit and bring up to about 30%.

With respect to original production flow process 2, to handle through this case, the mycillin of cancelling former flow process adds, and can improve fruiting bodies of cordyceps militaris or fermentation value of finished goods 50-200%.

Person skilled in the art should be familiar with; The above embodiment is used for explaining the object of the invention; And be not with opposing qualification of the present invention; As long as in essential scope of the present invention, variation, modification that the foregoing description is done all will drop in the claim scope of the present invention.

Claims (1)

1. pollution-free Cordyceps militaris spawn production or fermentation process comprise that step is following:

The biologic product that will help the Cordyceps militaris spawn fermentation adds water and mixes fully formation biological solutions, and the filtration back is subsequent use;

Medium batching adds the water constant volume, adds biological solutions afterwards, pass through autoclaving, aseptic inoculation step then after, carry out constant temperature culture.

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