CN102498942A - Method for producing oyster mushroom strains from birch chopsticks - Google Patents
Method for producing oyster mushroom strains from birch chopsticks Download PDFInfo
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- CN102498942A CN102498942A CN2011103435406A CN201110343540A CN102498942A CN 102498942 A CN102498942 A CN 102498942A CN 2011103435406 A CN2011103435406 A CN 2011103435406A CN 201110343540 A CN201110343540 A CN 201110343540A CN 102498942 A CN102498942 A CN 102498942A
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Abstract
The invention discloses a method for producing oyster mushroom strains from birch chopsticks. The method comprises four steps of: performing pretreatment on the chopsticks, preparing a culture medium for the chopsticks, performing inoculation culture and transferring the strains. According to the method disclosed by the invention, by carrying out pretreatment on the birch chopsticks, on the one hand, harmful substances in the birch chopsticks can be fully decomposed, and on the other hand, convenience is brought to the insertion of the birch chopsticks into a mycelium mass during the inoculation. When a culture medium for the birch chopsticks is prepared, as birch contains a large number of nutrient components required for growth of oyster mushrooms, only sucrose, a compound fertilizer, wheat bran and corn flour need to be further added, therefore the components in a nutrient solution are greatly simplified and the preparation process is also trouble-saving and effort-saving. When the inoculation is performed, only the chopsticks need to be directly inserted into the mycelium mass for completion, the operation process is convenient, fast, effort-saving and labor-saving and the pollution rate is very low. Mycelia are fully grown all over the whole chopstick strains after culture, the cell ages after the inoculation are short and consistent, the strains are fast in growth speed, the mycelia are robust, and the strains are high in purity and strong in resistance to other fungi. The method disclosed by the invention further has the more prominent advantages of low production cost and very significant economic benefits.
Description
Technical field
The present invention relates to the technology of preparing of flat mushroom strain, be specifically related to use disposable birch chopsticks to prepare the secondary kind of flat mushroom and the method for three grades of kinds as main raw material(s).
Background technology
At present, secondary kind and three grades of kinds that bag is planted flat mushroom are mainly granular bacteria strain, like wood chip bacterial classification, grain spawn, corncob bacterial classification etc.The granular bacteria strain proterties is stablized, and anti-hybrid ability is strong, and the inoculation operation is complicated, the inoculation work efficiency is low, time-consuming, production cost is higher but exist, and occupation of land is many, and cultivation cycle is long.
Summary of the invention
For overcoming the deficiency that prior art exists, the present invention provides that a kind of anti-hybrid ability is strong, fast growing bacteria and easy easy-to-use birch chopsticks are produced the flat mushroom strain method.
It is following that birch chopsticks of the present invention are produced the flat mushroom strain method step:
1) the chopsticks preliminary treatment is rejected substandard products crooked, cracking with disposable birch chopsticks, divides chopsticks jaw to fine away, and immerses in 5% limewash and soaks 20-24 hour, chopsticks is immersed in the clear water again, feeds 1500mg/h ozone 30-60 minute;
2) preparation chopsticks medium will pass through pretreated chopsticks and immerse and contain 5.5% glucose, 1.9% sucrose, 0.12% V
B1, 0.08% V
B9, in 0.2% the potassium dihydrogen phosphate, 0.2% magnesium sulfate solution, water temperature remains on 50-70 ℃, soak 1-2 hour after; Pull out and drain away the water, when airing to surface micro is done, put into the compound that 50% wheat bran adds 50% corn flour; Stir; Make chopsticks be stained with compound all over the body, then the chopsticks bunchy is tied up pack, the autoclaving sterilization is 1.5-2 hour under 121 ℃ of conditions;
3) the chopsticks bacterium bag after inoculated and cultured will be sterilized inserts flat mushroom strain with conventional method under aseptic condition; Then the chopstick strain bag was cultivated 15-20 days under 24-26 ℃ of constant temperature; The chopstick strain whole body just covers with mycelia; Transferred under the 10-11 ℃ of constant temperature rejuvenation 4-5 days, birch chopsticks flat mushroom strain is promptly processed;
4) after the switching bacterial classification is got the bacterium bag ready by the routine requirement, whole pair of chopsticks of cultured birch chopsticks flat mushroom strain are directly inserted in the bacterium bag, promptly accomplish inoculation.
Beneficial effect of the present invention: birch chopsticks of the present invention are produced the flat mushroom strain method, through the preliminary treatment to the birch chopsticks, harmful substance are decomposed fully, insert the bacterium bag when being convenient to inoculate on the other hand.During preparation birch chopsticks medium, because birch itself contains the nutrient component of a large amount of mushroom growth needs, therefore, only need to add a small amount of macroelement, vitamin, wheat bran and corn flour again, simplified the culture fluid composition greatly, the preparation process is the save trouble and labor also.Only need during inoculation that chopsticks are directly inserted the bacterium bag and just accomplish, convenient and swift, laborsaving saving of labor, and pollution rate is lower.Just cover with mycelia through cultivating the chopstick strain whole body; Inoculation back cell age is short, and the long speed of bacterial classification is fast and consistent, and mycelia is sturdy; Bacterial classification purity is high; Anti-hybrid ability is strong, has avoided conventional one-sided inoculation to occur growing after bacterium bag bottom is grown on bacterium bag top earlier, the aging phenomenon in bacterium bag top when bacterium bag bottom also there is not length good.The more outstanding advantage of the present invention is that production cost is low, from following several respects the present invention and conventional granulates bacterial classification is compared; (1) raw material: every bag of 100 pairs of chopstick strains of birch chopsticks flat mushroom strain of the present invention; Can inoculate 100 bags of three grades of kinds; And be example with wood chip conventional granulates bacterial classification, inoculate 8 bottles of secondary kinds of 100 bags of three grades of kind needs, about 3 yuan of every bag of cost of birch chopsticks flat mushroom strain; About 2 yuan of every bottle of cost of wood chip conventional granulates bacterial classification; The cost of inoculating 100 bags three grades kind birch chopsticks flat mushroom strain secondary kinds is 3 yuan, and the cost of wood chip conventional granulates bacterial classification secondary kind is 16 yuan, and the cost of raw material of wood chip bacterial classification is 5.3 times of chopstick strain.When (2) artificial: birch chopsticks flat mushroom strain inoculation operation of the present invention is very easy; Inoculation speed is very fast; Be example to inoculate 100 bags of three grades of kinds equally; Inoculation recruitment of the present invention be 0.3 people, hour, and the inoculation recruitment of wood chip conventional granulates bacterial classification be 2.5 people, hour, be 8.3 times of chopstick strain during wood chip bacterial classification artificial.(3) take up room: like calculating of raw material; The quantity of wood chip bacterial classification is 8 times of chopstick strain; It is slow that the wood chip bacterial classification is sent out bacterium, and the average time spent of secondary kind is about 30 days, and the birch chopstick strain only needed to use in 20 days; The former 1.5 times of taking up space than the latter more, two add up to the wood chip bacterial classification to take up room to be chopstick strain 12 times.
This shows that the resultant effect of birch chopsticks production flat mushroom strain method of the present invention is outstanding especially.
Embodiment
Produce the flat mushroom strain method with disposable birch chopsticks, divide chopsticks preliminary treatment, preparation chopsticks medium, inoculated and cultured and four steps of switching bacterial classification to accomplish.(1) the chopsticks preliminary treatment is rejected substandard products crooked, cracking with disposable birch chopsticks, divides chopsticks jaw to fine away, and immerses in 5% limewash and soaks 20-24 hour, chopsticks is immersed in the clear water again, feeds 1500mg/h ozone 30-60 minute; (2) preparation chopsticks medium will pass through pretreated chopsticks and immerse and contain 5.5% sucrose, 1.9 glucose, 0.12% V
B1, 0.08% V
B9, in 0.2% the potassium dihydrogen phosphate, 0.2% magnesium sulfate solution, water temperature remains on 50-70 ℃, soak 1-2 hour after; Pull out and drain away the water, when airing to surface micro is done, put into the compound that 50% wheat bran adds 50% corn flour; Stir; Make chopsticks be stained with compound all over the body, then the chopsticks bunchy is tied up pack, the autoclaving sterilization is 1.5-2 hour under 121 ℃ of conditions; (3) the chopsticks bacterium bag after inoculated and cultured will be sterilized inserts flat mushroom strain with conventional method under aseptic condition; Then the chopstick strain bag was cultivated 15-20 days under 24-26 ℃ of constant temperature; The chopstick strain whole body just covers with mycelia; Transferred under the 10-11 ℃ of constant temperature rejuvenation 4-5 days, birch chopsticks flat mushroom strain is promptly processed; (4) after the switching bacterial classification is got the bacterium bag ready by the routine requirement, whole pair of chopsticks of cultured birch chopsticks flat mushroom strain are directly inserted in the bacterium bag, promptly accomplish inoculation.
Claims (1)
1. the birch chopsticks are produced the flat mushroom strain method, and step is following:
1) the chopsticks preliminary treatment is rejected substandard products crooked, cracking with disposable birch chopsticks, divides chopsticks jaw to fine away, and immerses in 5% limewash and soaks 20-24 hour, chopsticks is immersed in the clear water again, feeds 1500mg/h ozone 30-60 minute;
2) preparation chopsticks medium will pass through pretreated chopsticks and immerse and contain 5.5% glucose, 1.9% sucrose, 0.12% V
B1, 0.08% V
B9, in 0.2% the potassium dihydrogen phosphate, 0.2% magnesium sulfate solution, water temperature remains on 50-70 ℃, soak 1-2 hour after; Pull out and drain away the water, when airing to surface micro is done, put into the compound that 50% wheat bran adds 50% corn flour; Stir; Make chopsticks be stained with compound all over the body, then the chopsticks bunchy is tied up pack, the autoclaving sterilization is 1.5-2 hour under 121 ℃ of conditions;
3) the chopsticks bacterium bag after inoculated and cultured will be sterilized inserts flat mushroom strain with conventional method under aseptic condition; Then the chopstick strain bag was cultivated 15-20 days under 24-26 ℃ of constant temperature; The chopstick strain whole body just covers with mycelia; Transferred under the 10-11 ℃ of constant temperature rejuvenation 4-5 days, birch chopsticks flat mushroom strain is promptly processed;
4) after the switching bacterial classification is got the bacterium bag ready by the routine requirement, whole pair of chopsticks of cultured birch chopsticks flat mushroom strain are directly inserted in the bacterium bag, promptly accomplish inoculation.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011103435406A CN102498942A (en) | 2011-11-04 | 2011-11-04 | Method for producing oyster mushroom strains from birch chopsticks |
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| CN2011103435406A CN102498942A (en) | 2011-11-04 | 2011-11-04 | Method for producing oyster mushroom strains from birch chopsticks |
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| CN102498942A true CN102498942A (en) | 2012-06-20 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103707376A (en) * | 2013-12-12 | 2014-04-09 | 重庆市福鑫洋食用菌有限公司 | Method for rapidly culturing edible fungus stock by using wood pole |
| CN106085883A (en) * | 2016-07-19 | 2016-11-09 | 陈毅忠 | A kind of preparation method utilizing waste wood chopsticks to prepare culture medium of edible fungus |
| CN106538242A (en) * | 2016-11-01 | 2017-03-29 | 陈天泰 | A kind of willow section edible mushroom cultivated species compost and cultigen preparation method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103707376A (en) * | 2013-12-12 | 2014-04-09 | 重庆市福鑫洋食用菌有限公司 | Method for rapidly culturing edible fungus stock by using wood pole |
| CN103707376B (en) * | 2013-12-12 | 2016-06-08 | 重庆市福鑫洋食用菌有限公司 | The method of rod fast culture edible fungi original seed |
| CN106085883A (en) * | 2016-07-19 | 2016-11-09 | 陈毅忠 | A kind of preparation method utilizing waste wood chopsticks to prepare culture medium of edible fungus |
| CN106538242A (en) * | 2016-11-01 | 2017-03-29 | 陈天泰 | A kind of willow section edible mushroom cultivated species compost and cultigen preparation method |
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Application publication date: 20120620 |