CN103707376B - The method of rod fast culture edible fungi original seed - Google Patents
The method of rod fast culture edible fungi original seed Download PDFInfo
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Abstract
The present invention be more particularly directed to a kind of method utilizing rod fast culture edible fungi original seed. The method flow process is: rod pre-treatment-prepare culture material-rod by formula rate mixes-pack or bottle-sterilizing-inoculation-bacteria-use or temporarily store with culture material. Described rod pre-treatment first makes rod soak solution, the clean rod of cleaning is put into rod soak solution and soaks; Semen Maydis powder is broken into Semen Maydis powder, according to water 1000ml: the ratio of Semen Maydis powder 300-500g mixes, heated and boiled is brewed into mashed prod and obtains cornmeal mush, adds the activity charcoal powder being equivalent to Semen Maydis powder quality 1/5-1/3 in cornmeal mush, stirs even that corn gac is stuck with paste; Again the rod after immersion is put into corn gac to stick with paste, stick with paste evenly wrapping up in last layer corn gac around rod, obtain pre-treatment rod. The method original seed of the present invention makes quick, shortens and makes the bacterial classification cycle, and strain quality is good, bacterial classification cultured continuously time lengthening and not aging, preservation period extends.
Description
Technical field
The invention belongs to edible mushrooms technical field, it relates to the cultural method of edible fungi original seed.
Background technology
Edible fungus species refers to and is separated a large amount of edible mushrooms of nature from deriving from and filters out useful bacterial classification, being improved, stored for future use refers to spore in production, original meaning again, but in actual production, often the pure mycelium through artificial culture is together called bacterial classification together with culture medium. Source according to bacterial classification in production, reproductive order of generation and production object, be divided into female kind bacterial classification, and original seed and cultivar, be again one-level kind respectively, two grades of kinds and three grades of kinds. Female kind: pure mycelium or spore are bred in Tube propagation base and become, it is possible to breeding original seed, also suitable culture presevation. Original seed: female kind mycelia breeds on solid medium and becomes, and this process strengthens mycelia to the adaptability of culture environment, plays again and expands numerous effect, and original seed can directly go out mushroom. Cultivar: become by protospecies breeding, mainly numerous in order to expand, for producing the bacterial classification providing enough.
Edible Fungi has less investment, high efficiency, can make full use of the advantages such as time in slack season, and development was very fast in recent years. But often can run into some problems and sometimes ignore a very little link tend and cause very big loss making in bacterial classification process. The bacterial initial species making of edible mushrooms is the prerequisite of edible fungus culturing, and the original seed that purity height, vitality are strong is the prerequisite that edible fungus culturing obtains high yield high-quality. The quality of edible fungi original seed quality is not only related to economic benefit height and the prestige quality of a kind bacterium field, and is related to the cultivation benefit of vast mushroom agriculture. Along with the great development of mushroom industry, the turnout of bacterial classification constantly expands, and the quality of edible fungus species original seed and a bacterium time have become edible mushrooms and developed very important problem. In Edible Fungi, the cultivation of original seed generally adopts the way of wood chip plus wheat bran, and this method takes a lot of work, time-consuming, sending out bacterium slow, the time is long, generally needs about 30d just can use, plantation family, in order to catch up with season, race against time, needs the practical technology that can be bred as original seed within the short period of time especially.
Existing document has reported technology that multiple eating bacterium original seed is bred as fast, as land-reclaimable academy of sciences of Heilongjiang Province biotechnology center has delivered one section " edible fungi original seed is bred as technology fast " (" modernization agricultural " on " modernization agricultural " periodical, 2nd phase in 2002 (total 271st phase) the 016th page) article, wherein describe a kind of method taking corn as culture material and making edible fungi original seed: select fresh, totally, without the corn grain gone mouldy, 12h (the most handy hot-water soak in winter is soaked with clear water, soak time is a little longer), boil to without white hard core with pot again, namely thoroughly well cooked but not mushy, then repeatedly rinse with cold water, wash thick liquid off, it is made to be shot shape, spread the 2��3h that dries in the air out, remove surface-moisture, load in glucose bottle (or wine bottle), corn grain is filled to the 2/3 of bottle height. autoclaving, pressure is 127.4��1137.2kPa, sterilizing 50min, atmospheric cooking sterilizing, 100 DEG C are steamed 6��8h. after sterilizing, material bottle temperature is dropped to less than 30 DEG C and can connect bacterium. this edible fungi original seed manufacture technology, draws materials easily, manufactures simple, sends out bacterium time relatively ordinary method shortening about 20d (mushroom class, auricularia auriculajudae, hedgehog hydnum shorten about 15d). substratum nutrition is abundant, and mycelial growth is vigorous. after proceeding to cultivar, sprouting fast, surviving rate height, sowing quantity is few. application number be 200910244881.0 Chinese patent report a kind of edible mushrooms two grades of kind (original seed) culture medium prescriptions and making method, taking haydite as main starting material, make edible mushrooms two grades of kind (original seed) substratum, culture medium prescription is: particle diameter is the haydite 75-85 jin of 0.5-1cm, wheat bran 14-24 jin, rice husk 14-24 jin, gypsum 1-2 jin, haydite need to soak with haydite soak solution, and haydite soak solution is that potato, glucose and water are formulated according to the ratio of 200g:20g:1000ml, use the method bacterial classification make quick, strain quality better, also has joint grain, the advantage such as efficient. application number be 201210011797.6 patent of invention disclose the making method of a kind of edible fungi original seed substratum, choose wheat, terra alba and calcium carbonate as raw material, first after wheat being soaked 24 hours with the clarification liming of 2%, picking up wheat clear water to rinse well, then be placed in water and boil, boiling time is 15��20 minutes, add terra alba and calcium carbonate again, stirring evenly, naturally cooling, can obtain required substratum, described wheat, terra alba and calcium carbonate mass ratio used is wheat 96%, terra alba 2% and calcium carbonate 2%, the method is practical, and cost of manufacture is low, and making method is simple, is convenient to penetration and promotion application, as substratum, can improve the output of edible mushrooms.
In above-mentioned method, the original seed made by culture material of wood chip, cotton seed hulls etc., needs into block and uses when inoculating and use, otherwise the fracture of bacterial classification mycelium, bacterial classification is sprouted relatively slow, directly affects the quality of three grades of kinds. , in addition it is also necessary to separately ward off path, therefore find and be more conducive to bacterial classification sprouting and protect bacterial classification mycelium not to be destroyed when using and divide and plant, reach the object reducing breeding cost raising strain quality and output.
Summary of the invention
In view of this, it is an object of the invention to provide a kind of method utilizing rod fast culture edible fungi original seed, this method reduce breeding cost, shorten bacterial classification fabrication cycle.
For realizing above-mentioned technical purpose, the technical scheme of the present invention is:
Rod pretreatment process, comprises the step carried out as follows:
(1) trees or bamboo sheet being made thick 0.5-0.6cm, the rod of long 10-15cm, described rod is put into clear water, ultrasonic cleaning is clean, for subsequent use;
(2) the clean rod of cleaning in step (1) is put into the immersion of rod soak solution and it is no less than 60 points of kinds, pick up rod after immersion and drain stand-by; Described rod soak solution is according to water 1000ml: sucrose 25-40g: unslaked lime 5-10g: potassium primary phosphate 2-5g: the ratio of magnesium sulfate 1-5g is prepared;
(3) select fresh, clean, carry out pulverizing to obtain Semen Maydis powder without the corn that goes mouldy, by described Semen Maydis powder with water according to water 1000ml: the ratio of Semen Maydis powder 300-500g mixes, heated and boiled is brewed into mashed prod and obtains cornmeal mush, add, in gained cornmeal mush, the activity charcoal powder being equivalent to Semen Maydis powder quality 1/5-1/3, stir even that corn gac is stuck with paste;
(4) the corn gac that the rod drained in step (2) is put into step (3) gained is stuck with paste, and sticks with paste evenly wrapping up in last layer corn gac around rod, obtains pre-treatment rod.
Further, described rod pretreatment process, in described step (1), described trees are the raw broadleaf tree of 1-2.
Further, described rod pretreatment process, in described step (1), described trees are the trees entering resting stage.
Further, described rod pretreatment process, in described step (2), puts into rod soak solution by cleaning clean rod in step (1), and the ultrasonication of 40-60kHZ heated and boiled are soaked and be no less than 60 points of kinds.
Further, described rod pretreatment process, in described step (2), puts into rod soak solution by cleaning clean rod in step (1), the ultrasonication of 40-60kHZ and heated and boiled soaks 80 points of kinds.
Further, described rod pretreatment process, in described step (2), described rod soak solution is also containing the chitosan being equivalent to sucrose quality 1/5-2/5.
Further, described rod pretreatment process, in described step (3), described corn pulverizes together with maize spike stalk.
Further, described rod pretreatment process, in described step (3), the particle diameter of described Semen Maydis powder is 100-200 order.
The method of rod fast culture edible fungi original seed, comprises the step carried out as follows:
1) rod pre-treatment
Trees or bamboo sheet are made thick 0.5-0.6cm, the rod of long 10-15cm, carry out processing to obtain pre-treatment rod according to the rod pretreatment process described in above-mentioned arbitrary item;
2) disinfection inoculation
Suitable culture material formula is selected according to selecting the wood-decay fungi cultivated, conveniently require to prepare culture material according to formula, by step 1) in the pre-treatment rod that obtains roll in described culture material, load one by one after rod is stained with culture material in plastics bag or vial, after filling, plastics bag or vial are carried out conventional sterilizing and inoculation, plastics bag or vial be placed under 20-25 DEG C of temperature environment lucifuge bacteria after inoculation, plastics bag or full bottle can be covered with through 15-20 days, become original seed.
Sterilizing is wanted thoroughly, and whether sterilizing is thoroughly related to the success or failure making bacterial classification, it suffices to say that the core of the making of edible fungus species is exactly that sterilizing is thorough; After sterilizing, bacterium bag temperature can not be inoculated higher than 28 DEG C, some mushroom agricultures only armrest touch bacterium bag surface, feeling that temperature does not start inoculation in higher position, after bacterial classification accesses, the waste heat at Jun Dai center is released gradually, bacterium bag bacterial classification is heated too high and dead, so when inoculating, must cool thoroughly.
Through 15-20 days, bacterial classification covered with plastics bag or full bottle, can continue to cultivate 20-30 days again, and the strain quality made like this can be better, more desirable for making the effect of cultivar.
The bacterial classification cultivated is put in about 4 DEG C, and dry, lucifuge place preserves, and it can not be made to be in has in the bigger environment becoming temperature, and the bacterial classification generally made preferably uses in 15d, and the shelf time does not exceed 30d.
The bacterial classification that the method for the present invention makes, because the cultured continuously time can reach about 45 days, bacterial classification is not aging, is equivalent to as longer 20-30 days in the fungi preservation phase utilizing wood chip to make than usual way.
The useful effect of the present invention: the major advantage of the present invention has: (1) rod wide material sources, enormous amount, current major part is all discarded or is only made firewood, causes significant wastage, uses it for cultivation edible fungi original seed, can reduce the cost of manufacture of original seed. (2) rod has been carried out pre-treatment by the present invention, pretreated rod is for cultivating edible fungi original seed, substratum permeability is good, cultivate at 20-25 DEG C, mycelial growth is rapid, within about 15-20 days, just covers with full culturing bottle, and incubation time shortens 10-15 days than conventional original seed, thus shorten cell age, extremely it is suitable for dashing off edible fungus species. (3) bacterial classification that the method for the present invention makes, the cultured continuously time can reach about 45 days and not aging, and Primary spawn time lengthening, is equivalent to as longer 20-30 days in the fungi preservation phase utilizing wood chip to make than usual way.
Embodiment
In order to make the object, technical solutions and advantages of the present invention clearly, below the preferred embodiments of the present invention are described in detail.
Wood-decay fungi has the ability decomposing xylogen, Mierocrystalline cellulose in timber, can be glucose, amino acid etc. by its decomposition and inversion, the nutritive substance directly absorbing as hyphal cell and utilizing.
Embodiment 1
1. rod pre-treatment
(1) choosing that the 1-2 entering resting stage is raw, the poplar-branch of thick about 10 millimeters, branch is gone epidermis, makes thick 0.5-0.6cm, the rod of long 10-15cm, described rod is put into clear water, ultrasonic cleaning is clean, for subsequent use;
(2) put into rod soak solution immersion 120 points of kinds by step (1) is cleaned clean rod, pick up rod after immersion and drain stand-by; Described rod soak solution is according to water 1000ml: sucrose 30g: unslaked lime 6g: potassium primary phosphate 3g: the ratio of magnesium sulfate 3g is prepared;
(3) select fresh, clean, carry out pulverizing to obtain Semen Maydis powder without the corn that goes mouldy, by described Semen Maydis powder with water according to water 1000ml: the ratio of Semen Maydis powder 300g mixes, heated and boiled is brewed into mashed prod and obtains cornmeal mush, add, in gained cornmeal mush, the activity charcoal powder being equivalent to Semen Maydis powder quality 1/3, stir even that corn gac is stuck with paste;
(4) the corn gac that the rod drained in step (2) is put into step (3) gained is stuck with paste, and sticks with paste evenly wrapping up in last layer corn gac around rod, obtains pre-treatment rod.
2. disinfection inoculation
The wood-decay fungi that the present embodiment is selected is mushroom strain, culture material formula is: wood chip 78%, wheat bran 20%, sucrose 1%, gypsum 1%, conveniently require to prepare culture material according to formula, by step 1) in the pre-treatment rod that obtains roll in described culture material, load one by one in vial after rod is stained with culture material, after filling, vial bottleneck is wrapped with clean kraft paper, carry out normal-pressure sterilization 8 hours, treat after sterilizing that vial temperature is down to less than 28 DEG C, open kraft paper and carry out conventional inoculation, after inoculation, vial bottleneck still wraps kraft paper, vial is placed under 20-25 DEG C of temperature environment lucifuge bacteria, namely full bottle is covered with through 20 days, become original seed.Namely original seed now can be used for the making of cultivar, if after bacterial classification covers with full bottle, then continues to cultivate 20-30 days, and the strain quality made like this can be better, more desirable for making the effect of cultivar. The bacterial classification cultivated is put in about 4 DEG C, and dry, lucifuge place preserves, and it can not be made to be in has in the bigger environment becoming temperature, and the bacterial classification generally made preferably uses in 15d, and the shelf time does not exceed 30d; And with the bacterial classification that the method for the present invention makes, because the cultured continuously time can reach about 45 days, be equivalent to as longer 20-30 days in the fungi preservation phase utilizing wood chip to make than usual way.
Embodiment 2
1. rod pre-treatment
(1) choosing that 1-2 is raw, the willow branch of thick about 10 millimeters, branch is gone epidermis, makes thick 0.5-0.6cm, the rod of long 10-15cm, described rod is put into clear water, ultrasonic cleaning is clean, for subsequent use;
(2) put into rod soak solution by step (1) is cleaned clean rod, the ultrasonication of 40-60kHZ and heated and boiled soaks 80 points of kinds, pick up rod after immersion and drain stand-by; Described rod soak solution is according to water 1000ml: sucrose 35g: unslaked lime 8g: potassium primary phosphate 4g: the ratio of magnesium sulfate 4g is prepared;
(3) select fresh, clean, carry out pulverizing to obtain Semen Maydis powder without the corn that goes mouldy, by described Semen Maydis powder with water according to water 1000ml: the ratio of Semen Maydis powder 450g mixes, heated and boiled is brewed into mashed prod and obtains cornmeal mush, add, in gained cornmeal mush, the activity charcoal powder being equivalent to Semen Maydis powder quality 1/4, stir even that corn gac is stuck with paste;
(4) the corn gac that the rod drained in step (2) is put into step (3) gained is stuck with paste, and sticks with paste evenly wrapping up in last layer corn gac around rod, obtains pre-treatment rod.
2. disinfection inoculation
The wood-decay fungi that the present embodiment is selected is black fungus bacterial classification, culture material formula is: wheat bran 78%, rice bran 20%, sucrose 1%, gypsum 1%, conveniently require to prepare culture material according to formula, by step 1) in the pre-treatment rod that obtains roll in described culture material, load one by one in vial after rod is stained with culture material, after filling, vial bottleneck is wrapped with clean kraft paper, carry out autoclaving 2 hours, pressure is 130kPa, treat after sterilizing that vial temperature is down to less than 28 DEG C, open kraft paper and carry out conventional inoculation, after inoculation, vial bottleneck still wraps kraft paper, vial is placed under 20-25 DEG C of temperature environment lucifuge bacteria, namely full bottle is covered with through 20 days, become original seed. namely original seed now can be used for the making of cultivar, if after bacterial classification covers with full bottle, then continues to cultivate 20-30 days, and the strain quality made like this can be better, more desirable for making the effect of cultivar. the bacterial classification cultivated is put in about 4 DEG C, and dry, lucifuge place preserves, and it can not be made to be in has in the bigger environment becoming temperature, and the bacterial classification generally made preferably uses in 15d, and the shelf time does not exceed 30d, and with the bacterial classification that the method for the present invention makes, because the cultured continuously time can reach about 45 days, be equivalent to as longer 20-30 days in the fungi preservation phase utilizing wood chip to make than usual way.
Embodiment 3
1. rod pre-treatment
(1) choosing that 1-2 is raw, the willow branch of thick about 10 millimeters, branch is gone epidermis, makes thick 0.5-0.6cm, the rod of long 10-15cm, described rod is put into clear water, ultrasonic cleaning is clean, for subsequent use;
(2) put into rod soak solution by step (1) is cleaned clean rod, the ultrasonication of 40-60kHZ and heated and boiled soaks 80 points of kinds, pick up rod after immersion and drain stand-by;Described rod soak solution is according to water 1000ml: sucrose 35g: unslaked lime 8g: potassium primary phosphate 4g: the ratio of magnesium sulfate 4g is prepared; Rod soak solution also adds the chitosan being equivalent to sucrose quality 2/5.
(3) select fresh, clean, without the corn that goes mouldy, described corn comprises maize spike stalk, it is 100 orders by crush maize to particle diameter, obtain Semen Maydis powder, by described Semen Maydis powder with water according to water 1000ml: the ratio of Semen Maydis powder 450g mixes, heated and boiled is brewed into mashed prod and obtains cornmeal mush, adds, in gained cornmeal mush, the activity charcoal powder being equivalent to Semen Maydis powder quality 1/4, stirs even that corn gac is stuck with paste;
(4) the corn gac that the rod drained in step (2) is put into step (3) gained is stuck with paste, and sticks with paste evenly wrapping up in last layer corn gac around rod, obtains pre-treatment rod.
2. disinfection inoculation
The wood-decay fungi that the present embodiment is selected is black fungus bacterial classification, culture material formula is: wheat bran 78%, rice bran 20%, sucrose 1%, gypsum 1%, conveniently require to prepare culture material according to formula, by step 1) in the pre-treatment rod that obtains roll in described culture material, load one by one in vial after rod is stained with culture material, after filling, vial bottleneck is wrapped with clean kraft paper, carry out autoclaving 2 hours, pressure is 130kPa, treat after sterilizing that vial temperature is down to less than 28 DEG C, open kraft paper and carry out conventional inoculation, after inoculation, vial bottleneck still wraps kraft paper, vial is placed under 20-25 DEG C of temperature environment lucifuge bacteria, namely full bottle is covered with through 15 days, become original seed. namely original seed now can be used for the making of cultivar, if after bacterial classification covers with full bottle, then continues to cultivate 20-30 days, and the strain quality made like this can be better, more desirable for making the effect of cultivar. the bacterial classification cultivated is put in about 4 DEG C, and dry, lucifuge place preserves, and it can not be made to be in has in the bigger environment becoming temperature, and the bacterial classification generally made preferably used in 15 days, and the shelf time does not exceed 30 days, and with the bacterial classification that the method for the present invention makes, because the cultured continuously time can reach about 45 days, be equivalent to as longer 20-30 days in the fungi preservation phase utilizing wood chip to make than usual way.
Cultivating edible fungi original seed with rod, the permeability of substratum is good, cultivates at 20-25 DEG C, mycelial growth is rapid, within about 15-20 days, just covers with full culturing bottle, and incubation time shortens 10-15 days than conventional original seed, thus shorten cell age, extremely it is suitable for dashing off wood-decay fungi bacterial classification.
Because rod surface-area is big, mycelia, except growth is except branch surface, also can be grown in rod, and the hyphae length carried is big, is just increased inoculum size undoubtedly when transferring cultivar. When transferring wood chip, husk class cultivar, only need to pick up in the middle of a rod insertion substratum with aseptic nipper, inoculate simply easy to operate, be conducive to pollution abatement, increase success ratio of inoculation. After inoculation, bacterial classification is sprouted from upper, middle and lower multiple spot, and mycelia outwards spreads from inner, and the full bottle purseful time generally can do sth. in advance 10-15 days. In addition, one bottle of original seed can connect the cultivar of 110-120 bag (bottle), and inoculation bag (bottle) number is many, saves original seed, comparatively economical.
What finally illustrate is, above embodiment is only in order to illustrate the technical scheme of the present invention and unrestricted, although with reference to better embodiment to invention has been detailed explanation, it will be understood by those within the art that, technical scheme can be modified or equivalent replacement, and not departing from objective and the scope of technical solution of the present invention, it all should be encompassed in the middle of the right of the present invention.
Claims (9)
1. rod pretreatment process, it is characterised in that, comprise the step carried out as follows:
(1) trees being made thick 0.5-0.6cm, the rod of long 10-15cm, puts into clear water by described rod, and ultrasonic cleaning is clean, for subsequent use;
(2) the clean rod of cleaning in step (1) is put into the immersion of rod soak solution and it is no less than 60 minutes, pick up rod after immersion and drain stand-by; Described rod soak solution is according to water 1000ml: sucrose 25-40g: unslaked lime 5-10g: potassium primary phosphate 2-5g: the ratio of magnesium sulfate 1-5g is prepared;
(3) select fresh, clean, carry out pulverizing to obtain Semen Maydis powder without the corn that goes mouldy, by described Semen Maydis powder with water according to water 1000ml: the ratio of Semen Maydis powder 300-500g mixes, heated and boiled is brewed into mashed prod and obtains cornmeal mush, add, in gained cornmeal mush, the activity charcoal powder being equivalent to Semen Maydis powder quality 1/5-1/3, stir even that corn gac is stuck with paste;
(4) the corn gac that the rod drained in step (2) is put into step (3) gained is stuck with paste, and sticks with paste evenly wrapping up in last layer corn gac around rod, obtains pre-treatment rod.
2. rod pretreatment process according to claim 1, it is characterised in that, in described step (1), described trees are the raw broadleaf tree of 1-2.
3. rod pretreatment process according to claim 2, it is characterised in that, in described step (1), described trees are the trees entering resting stage.
4. rod pretreatment process according to claim 1, it is characterized in that, in described step (2), putting into rod soak solution by cleaning clean rod in step (1), the ultrasonication of 40-60kHZ heated and boiled are soaked and are no less than 60 minutes.
5. rod pretreatment process according to claim 4, it is characterized in that, in described step (2), put into rod soak solution by step (1) is cleaned clean rod, the ultrasonication of 40-60kHZ and heated and boiled soaks 80 minutes.
6. rod pretreatment process according to claim 1, it is characterised in that, in described step (2), described rod soak solution is also containing the chitosan being equivalent to sucrose quality 1/5-2/5.
7. rod pretreatment process according to claim 1, it is characterised in that, in described step (3), described corn pulverizes together with maize spike stalk.
8. rod pretreatment process according to claim 1, it is characterised in that, in described step (3), the particle diameter of described Semen Maydis powder is 100-200 order.
9. the method for rod fast culture edible fungi original seed, it is characterised in that, comprise the step carried out as follows:
1) rod pre-treatment
Carry out processing to obtain pre-treatment rod according to the method described in the arbitrary item of claim 1-8;
2) disinfection inoculation
Suitable culture material formula is selected according to selecting the wood-decay fungi cultivated, then conveniently require to prepare culture material according to formula, by step 1) in the pre-treatment rod that obtains roll in described culture material, load one by one after rod is stained with culture material in plastics bag or vial, after filling, plastics bag or vial are carried out conventional sterilizing and inoculation, plastics bag or vial be placed under 20-25 DEG C of temperature environment lucifuge bacteria after inoculation, plastics bag or full bottle can be covered with through 15-20 days, become original seed.
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| CN104285677B (en) * | 2014-10-30 | 2016-03-30 | 武汉岁岁丰农业科技开发有限公司 | A kind of preparation method of edible mushroom peg wood bacterial classification |
| CN111448946A (en) * | 2020-06-01 | 2020-07-28 | 安徽省百麓现代农业科技有限公司 | Dictyophora rubrovalvata branch strain cultivation method |
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| CN101456769A (en) * | 2007-12-12 | 2009-06-17 | 辽宁东方农业科技有限公司 | Bag cultivation champignon chopstick strain raw material formula and production process |
| CN102422776A (en) * | 2011-09-08 | 2012-04-25 | 镇江市食用菌研究所 | Solid strain culture medium of edible fungi and production method of solid strain |
| CN102498942A (en) * | 2011-11-04 | 2012-06-20 | 顾环环 | Method for producing oyster mushroom strains from birch chopsticks |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2004065195A (en) * | 2002-08-09 | 2004-03-04 | Sangaku Renkei Kiko Kyushu:Kk | Mushroom bed for mushroom cultivation and method for producing the same |
| CN101456769A (en) * | 2007-12-12 | 2009-06-17 | 辽宁东方农业科技有限公司 | Bag cultivation champignon chopstick strain raw material formula and production process |
| CN102422776A (en) * | 2011-09-08 | 2012-04-25 | 镇江市食用菌研究所 | Solid strain culture medium of edible fungi and production method of solid strain |
| CN102498942A (en) * | 2011-11-04 | 2012-06-20 | 顾环环 | Method for producing oyster mushroom strains from birch chopsticks |
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