CN111448946A - Dictyophora rubrovalvata branch strain cultivation method - Google Patents
Dictyophora rubrovalvata branch strain cultivation method Download PDFInfo
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- CN111448946A CN111448946A CN202010483968.XA CN202010483968A CN111448946A CN 111448946 A CN111448946 A CN 111448946A CN 202010483968 A CN202010483968 A CN 202010483968A CN 111448946 A CN111448946 A CN 111448946A
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- branches
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- poplar branches
- poplar
- steep liquor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Abstract
The invention discloses a Dictyophora rubrovalvata branch strain cultivation method, which comprises the following steps: (1) selecting raw materials, (2) selecting branches, (3) treating branches, (4) preparing corn steep liquor, (5) boiling for the first time, (6) boiling for the second time, (7) mixing raw materials, (8) sterilizing and (9) inoculating. The invention utilizes poplar branches as carriers of strains, can disperse the strains when being placed in a culture medium, enables the strains to grow at 360 degrees, has the advantages of fast inoculation, germination and feed intake, consistent germination and good stability, can effectively shorten the strain development time, has consistent strain age, ensures the strain activity, has high inoculation speed, is suitable for large-scale production, and has the stability of solid strains and the inoculation speed of liquid strains.
Description
Technical Field
The invention relates to the technical field of edible fungi, in particular to a Dictyophora rubrovalvata branch strain cultivation method.
Background
Dictyophora rubrovalvata is a characteristic edible fungus in Guizhou, and can be artificially cultivated in a large scale. Dictyophora rubrovalvata is cultivated in China at present.
In the existing dictyophora rubrovolvata strain cultivation process, most of sawdust solid strains are used as main strains, the inoculation speed is low, the inoculation amount is difficult to control, the germination is slow, the pollution rate is high, the strains generally grow slowly from top to bottom in a culture medium, the growth speed is slow, the large-scale production is not suitable, the dictyophora rubrovolvata liquid strain production is unstable, and therefore, the dictyophora rubrovolvata branch strain cultivation method is provided.
Disclosure of Invention
The invention aims to provide a Dictyophora rubrovalvata branch strain cultivation method to solve the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: a Dictyophora rubrovalvata branch strain cultivation method comprises the following steps:
(1) selecting raw materials: firstly, selecting corresponding poplar branches, sawdust, corn flour, white granulated sugar, gypsum powder, potassium dihydrogen phosphate and magnesium sulfate according to corresponding parts by weight for later use;
(2) selecting branches: selecting fresh poplar branches according to the production quantity, then cutting the fresh poplar branches into the sizes of 4-6mm in width, 4-6mm in thickness and 130-150mm in length for later use, and checking whether the poplar branches are damaged by insects or mildew or not before use;
(3) branch treatment: soaking the fresh poplar branches selected in the step (2) in saturated lime water prepared in advance, wherein the poplar branches are completely soaked in the lime water in the soaking process for more than 48 hours until no white core exists in the poplar branches, and the soaked poplar branches are cleaned by clear water and drained for later use;
(4) preparing corn steep liquor: diluting the fresh mildew-free corn flour weighed in the step (1) with water, and soaking for 1-2h for later use after dilution is completed;
(5) primary boiling: adding the white granulated sugar, the gypsum powder, the potassium dihydrogen phosphate, the magnesium sulfate and the triacontanol which are weighed in the step (1) into water to be completely dissolved, and pouring the mixture into a boiling pot to be boiled to obtain a boiling solution A for later use;
(6) secondary boiling: pouring the corn steep liquor soaked in the step (4) into the boiling solution A in the step (5), continuously heating and boiling, continuously stirring after the corn steep liquor is poured until the corn steep liquor is completely cooked to form paste, and naturally cooling the corn steep liquor to below 25 ℃ for later use;
(7) mixing raw materials: pouring the wood chips weighed in the step (1) into a basin, then pouring the fresh poplar branches soaked in the step (3) into the corn steep liquor in the step (6), fishing out the outer surfaces of the poplar branches after the corn steep liquor is fully coated on the outer surfaces of the poplar branches, spraying the prepared wood chips on the outer surfaces of the poplar branches, filling the wood chips into a fungus bag after the wood chips are fully coated, and sealing the fungus bag;
(8) and (3) sterilization: placing the branch culture medium loaded in the step (7) into an autoclave for sterilization for 2h, then taking out, and naturally cooling the culture medium to perform inoculation;
(9) inoculation: selecting pure white, pollution-free and aging-free dictyophora rubrovolvata stock seeds, carrying out branch fungus bag inoculation on a super clean bench, then transferring the stock seeds to a constant-temperature strain culture chamber for culture, and fully distributing hyphae after culturing for 75-90 days.
Furthermore, the dictyophora rubrovolvata branch strain manufacturing raw materials comprise, by mass, 78-82% of poplar branches, 14-16% of sawdust, 2.5-3% of corn flour, 1-2% of white granulated sugar, 0.8-1.2% of gypsum powder and 0.3-0.7% of monopotassium phosphate.
Further, the size of the fungus bag is 15 x 33cm, and the surface sealing of the fungus bag is performed by a 38mm lantern ring waterproof cover.
The invention utilizes poplar branches as carriers of strains, can disperse the strains when being placed in a culture medium, enables the strains to grow at 360 degrees, has the advantages of fast inoculation, germination and feed intake, consistent germination and good stability, can effectively shorten the strain development time, has consistent strain age, ensures the strain activity, has high inoculation speed, is suitable for large-scale production, and has the stability of solid strains and the inoculation speed of liquid strains.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
a Dictyophora rubrovalvata branch strain cultivation method comprises the following steps:
(1) selecting raw materials: firstly, selecting corresponding poplar branches, sawdust, corn flour, white granulated sugar, gypsum powder, potassium dihydrogen phosphate and magnesium sulfate according to corresponding parts by weight for later use;
(2) selecting branches: selecting fresh poplar branches according to the production capacity, then cutting the fresh poplar branches into the sizes of 4mm in width, 4mm in thickness and 130mm in length for later use, and checking whether the poplar branches are damaged by insects or not before use;
(3) branch treatment: soaking the fresh poplar branches selected in the step (2) in saturated lime water prepared in advance, wherein the poplar branches are completely soaked in the lime water in the soaking process for more than 48 hours until no white core exists in the poplar branches, and the soaked poplar branches are cleaned by clear water and drained for later use;
(4) preparing corn steep liquor: diluting the fresh mildew-free corn flour weighed in the step (1) with water, and soaking for 1h for later use after dilution is completed;
(5) primary boiling: adding the white granulated sugar, the gypsum powder, the potassium dihydrogen phosphate, the magnesium sulfate and the triacontanol which are weighed in the step (1) into water to be completely dissolved, and pouring the mixture into a boiling pot to be boiled to obtain a boiling solution A for later use;
(6) secondary boiling: pouring the corn steep liquor soaked in the step (4) into the boiling solution A in the step (5), continuously heating and boiling, continuously stirring after the corn steep liquor is poured until the corn steep liquor is completely cooked to form paste, and naturally cooling the corn steep liquor to below 25 ℃ for later use;
(7) mixing raw materials: pouring the wood chips weighed in the step (1) into a basin, then pouring the fresh poplar branches soaked in the step (3) into the corn steep liquor in the step (6), fishing out the outer surfaces of the poplar branches after the corn steep liquor is fully coated on the outer surfaces of the poplar branches, spraying the prepared wood chips on the outer surfaces of the poplar branches, filling the wood chips into a fungus bag after the wood chips are fully coated, and sealing the fungus bag;
(8) and (3) sterilization: placing the branch culture medium loaded in the step (7) into an autoclave for sterilization for 2h, then taking out, and naturally cooling the culture medium to perform inoculation;
(9) inoculation: selecting a dictyophora rubrovolvata stock seed with white hypha, no pollution and no aging, carrying out branch fungus bag inoculation on a super clean bench, then transferring the stock seed to a constant-temperature strain culture chamber for culture, and spreading the hypha for use after culturing for 75 days.
Further, the dictyophora rubrovolvata branch strain manufacturing raw materials comprise, by mass, 81.4% of poplar branches, 14% of sawdust, 2.5% of corn flour, 1% of white granulated sugar, 0.8% of gypsum powder and 0.3% of potassium dihydrogen phosphate.
Further, the size of the fungus bag is 15 x 33cm, and the surface sealing of the fungus bag is performed by a 38mm lantern ring waterproof cover.
Example two:
a Dictyophora rubrovalvata branch strain cultivation method comprises the following steps:
(1) selecting raw materials: firstly, selecting corresponding poplar branches, sawdust, corn flour, white granulated sugar, gypsum powder, potassium dihydrogen phosphate and magnesium sulfate according to corresponding parts by weight for later use;
(2) selecting branches: selecting fresh poplar branches according to the production capacity, then cutting the fresh poplar branches into sizes with the width of 6mm, the thickness of 6mm and the length of 150mm for later use, and checking whether the poplar branches are damaged by insects or not before use;
(3) branch treatment: soaking the fresh poplar branches selected in the step (2) in saturated lime water prepared in advance, wherein the poplar branches are completely soaked in the lime water in the soaking process for more than 48 hours until no white core exists in the poplar branches, and the soaked poplar branches are cleaned by clear water and drained for later use;
(4) preparing corn steep liquor: diluting the fresh mildew-free corn flour weighed in the step (1) with water, and soaking for 2 hours for later use after dilution is completed;
(5) primary boiling: adding the white granulated sugar, the gypsum powder, the potassium dihydrogen phosphate, the magnesium sulfate and the triacontanol which are weighed in the step (1) into water to be completely dissolved, and pouring the mixture into a boiling pot to be boiled to obtain a boiling solution A for later use;
(6) secondary boiling: pouring the corn steep liquor soaked in the step (4) into the boiling solution A in the step (5), continuously heating and boiling, continuously stirring after the corn steep liquor is poured until the corn steep liquor is completely cooked to form paste, and naturally cooling the corn steep liquor to below 25 ℃ for later use;
(7) mixing raw materials: pouring the wood chips weighed in the step (1) into a basin, then pouring the fresh poplar branches soaked in the step (3) into the corn steep liquor in the step (6), fishing out the outer surfaces of the poplar branches after the corn steep liquor is fully coated on the outer surfaces of the poplar branches, spraying the prepared wood chips on the outer surfaces of the poplar branches, filling the wood chips into a fungus bag after the wood chips are fully coated, and sealing the fungus bag;
(8) and (3) sterilization: placing the branch culture medium loaded in the step (7) into an autoclave for sterilization for 2h, then taking out, and naturally cooling the culture medium to perform inoculation;
(9) inoculation: selecting a dictyophora rubrovolvata stock seed with white hypha, no pollution and no aging, carrying out branch fungus bag inoculation on a super clean bench, then transferring the stock seed to a constant-temperature strain culture chamber for culture, and fully spreading the hypha after culturing for 90 days.
Furthermore, the dictyophora rubrovolvata branch strain manufacturing raw materials comprise, by mass, 78.1% of poplar branches, 15% of sawdust, 3% of corn flour, 2% of white granulated sugar, 1.2% of gypsum powder and 0.7% of potassium dihydrogen phosphate.
Further, the size of the fungus bag is 15 x 33cm, and the surface sealing of the fungus bag is performed by a 38mm lantern ring waterproof cover.
Example three:
a Dictyophora rubrovalvata branch strain cultivation method comprises the following steps:
(1) selecting raw materials: firstly, selecting corresponding poplar branches, sawdust, corn flour, white granulated sugar, gypsum powder, potassium dihydrogen phosphate and magnesium sulfate according to corresponding parts by weight for later use;
(2) selecting branches: selecting fresh poplar branches according to the production quantity, then cutting the fresh poplar branches into the sizes of 4-6mm in width, 4-6mm in thickness and 130-150mm in length for later use, and checking whether the poplar branches are damaged by insects or mildew or not before use;
(3) branch treatment: soaking the fresh poplar branches selected in the step (2) in saturated lime water prepared in advance, wherein the poplar branches are completely soaked in the lime water in the soaking process for more than 48 hours until no white core exists in the poplar branches, and the soaked poplar branches are cleaned by clear water and drained for later use;
(4) preparing corn steep liquor: diluting the fresh mildew-free corn flour weighed in the step (1) with water, and soaking for 1-2h for later use after dilution is completed;
(5) primary boiling: adding the white granulated sugar, the gypsum powder, the potassium dihydrogen phosphate, the magnesium sulfate and the triacontanol which are weighed in the step (1) into water to be completely dissolved, and pouring the mixture into a boiling pot to be boiled to obtain a boiling solution A for later use;
(6) secondary boiling: pouring the corn steep liquor soaked in the step (4) into the boiling solution A in the step (5), continuously heating and boiling, continuously stirring after the corn steep liquor is poured until the corn steep liquor is completely cooked to form paste, and naturally cooling the corn steep liquor to below 25 ℃ for later use;
(7) mixing raw materials: pouring the wood chips weighed in the step (1) into a basin, then pouring the fresh poplar branches soaked in the step (3) into the corn steep liquor in the step (6), fishing out the outer surfaces of the poplar branches after the corn steep liquor is fully coated on the outer surfaces of the poplar branches, spraying the prepared wood chips on the outer surfaces of the poplar branches, filling the wood chips into a fungus bag after the wood chips are fully coated, and sealing the fungus bag;
(8) and (3) sterilization: placing the branch culture medium loaded in the step (7) into an autoclave for sterilization for 2h, then taking out, and naturally cooling the culture medium to perform inoculation;
(9) inoculation: selecting pure white, pollution-free and aging-free dictyophora rubrovolvata stock seeds, carrying out branch fungus bag inoculation on a super clean bench, then transferring the stock seeds to a constant-temperature strain culture chamber for culture, and fully distributing hyphae after culturing for 75-90 days.
Furthermore, the dictyophora rubrovolvata branch strain manufacturing raw materials comprise, by mass, 80% of poplar branches, 14.6% of sawdust, 2.8% of corn flour, 1.3% of white granulated sugar, 0.8% of gypsum powder and 0.5% of potassium dihydrogen phosphate.
Further, the size of the fungus bag is 15 x 33cm, and the surface sealing of the fungus bag is performed by a 38mm lantern ring waterproof cover.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (3)
1. A Dictyophora rubrovalvata branch strain cultivation method is characterized by comprising the following steps: the method for cultivating Dictyophora rubrovalvata branch strains comprises the following steps:
(1) selecting raw materials: firstly, selecting corresponding poplar branches, sawdust, corn flour, white granulated sugar, gypsum powder, potassium dihydrogen phosphate and magnesium sulfate according to corresponding parts by weight for later use;
(2) selecting branches: selecting fresh poplar branches according to the production quantity, then cutting the fresh poplar branches into the sizes of 4-6mm in width, 4-6mm in thickness and 130-150mm in length for later use, and checking whether the poplar branches are damaged by insects or mildew or not before use;
(3) branch treatment: soaking the fresh poplar branches selected in the step (2) in saturated lime water prepared in advance, wherein the poplar branches are completely soaked in the lime water in the soaking process for more than 48 hours until no white core exists in the poplar branches, and the soaked poplar branches are cleaned by clear water and drained for later use;
(4) preparing corn steep liquor: diluting the fresh mildew-free corn flour weighed in the step (1) with water, and soaking for 1-2h for later use after dilution is completed;
(5) primary boiling: adding the white granulated sugar, the gypsum powder, the potassium dihydrogen phosphate, the magnesium sulfate and the triacontanol which are weighed in the step (1) into water to be completely dissolved, and pouring the mixture into a boiling pot to be boiled to obtain a boiling solution A for later use;
(6) secondary boiling: pouring the corn steep liquor soaked in the step (4) into the boiling solution A in the step (5), continuously heating and boiling, continuously stirring after the corn steep liquor is poured until the corn steep liquor is completely cooked to form paste, and naturally cooling the corn steep liquor to below 25 ℃ for later use;
(7) mixing raw materials: pouring the wood chips weighed in the step (1) into a basin, then pouring the fresh poplar branches soaked in the step (3) into the corn steep liquor in the step (6), fishing out the outer surfaces of the poplar branches after the corn steep liquor is fully coated on the outer surfaces of the poplar branches, spraying the prepared wood chips on the outer surfaces of the poplar branches, filling the wood chips into a fungus bag after the wood chips are fully coated, and sealing the fungus bag;
(8) and (3) sterilization: placing the branch culture medium loaded in the step (7) into an autoclave for sterilization for 2h, then taking out, and naturally cooling the culture medium to perform inoculation;
(9) inoculation: selecting pure white, pollution-free and aging-free dictyophora rubrovolvata stock seeds, carrying out branch fungus bag inoculation on a super clean bench, then transferring the stock seeds to a constant-temperature strain culture chamber for culture, and fully distributing hyphae after culturing for 75-90 days.
2. The method for cultivating Dictyophora rubrovalvata branch strains according to claim 1, wherein the method comprises the following steps: the dictyophora rubrovolvata branch strain is prepared from 78-82% of poplar branches, 14-16% of sawdust, 2.5-3% of corn flour, 1-2% of white granulated sugar, 0.8-1.2% of gypsum powder and 0.3-0.7% of monopotassium phosphate in parts by mass.
3. The method for cultivating Dictyophora rubrovalvata branch strains according to claim 1, wherein the method comprises the following steps: the size of the fungus bag is 15 x 33cm polypropylene fungus bag, and the surface sealing of the fungus bag is performed by a 38mm lantern ring waterproof cover.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112400608A (en) * | 2020-11-09 | 2021-02-26 | 云南省农业技术推广总站 | Method for culturing and propagating strain after inoculation by utilizing mulberry branches and propagation device |
| CN113973645A (en) * | 2021-10-27 | 2022-01-28 | 贵州省山地资源研究所 | Method for preparing Dictyophora rubrovalvata branch seeds |
| CN115336502A (en) * | 2022-08-31 | 2022-11-15 | 贵州金荪生物科技开发有限责任公司 | Formula and preparation method of cultivation fungus sticks of dictyophora rubrovolvata |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1235755A (en) * | 1999-05-14 | 1999-11-24 | 安晨明 | Technology for producing dendritical spawn of bag cultivated edible mushroom |
| KR100717124B1 (en) * | 2006-09-04 | 2007-05-10 | 최경남 | Artificial cultivating method of dictyophora indusiata in the bare ground |
| CN102172173A (en) * | 2011-03-04 | 2011-09-07 | 广东省微生物研究所 | Method for cultivating and producing sporocarps of dictyophora multicolor berk. and broome |
| CN103392507A (en) * | 2013-08-10 | 2013-11-20 | 黄艳芳 | Method for preparing bar-shaped strain |
| CN103707376A (en) * | 2013-12-12 | 2014-04-09 | 重庆市福鑫洋食用菌有限公司 | Method for rapidly culturing edible fungus stock by using wood pole |
| CN104285677A (en) * | 2014-10-30 | 2015-01-21 | 武汉岁岁丰农业科技开发有限公司 | Method for making edible mushroom pegwood strain |
| CN104718969A (en) * | 2013-12-18 | 2015-06-24 | 于汇 | Production technology and using method for edible mushroom branch mother seeds |
| CN105237139A (en) * | 2015-08-29 | 2016-01-13 | 安远县天华现代农业有限责任公司 | Preparation and culturing method of tree branch bacterial strain of pleurotus eryngii and hypsizygus marmoreus |
| CN108739047A (en) * | 2018-05-10 | 2018-11-06 | 山东恒发食用菌有限公司 | A kind of production of Pleurotus nebrodensis tree fungus and breeding method |
| CN109156272A (en) * | 2018-08-31 | 2019-01-08 | 成都市宁升绿康食品有限公司 | Seafood mushroom cultivates tree fungus preparation process |
-
2020
- 2020-06-01 CN CN202010483968.XA patent/CN111448946A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1235755A (en) * | 1999-05-14 | 1999-11-24 | 安晨明 | Technology for producing dendritical spawn of bag cultivated edible mushroom |
| KR100717124B1 (en) * | 2006-09-04 | 2007-05-10 | 최경남 | Artificial cultivating method of dictyophora indusiata in the bare ground |
| CN102172173A (en) * | 2011-03-04 | 2011-09-07 | 广东省微生物研究所 | Method for cultivating and producing sporocarps of dictyophora multicolor berk. and broome |
| CN103392507A (en) * | 2013-08-10 | 2013-11-20 | 黄艳芳 | Method for preparing bar-shaped strain |
| CN103707376A (en) * | 2013-12-12 | 2014-04-09 | 重庆市福鑫洋食用菌有限公司 | Method for rapidly culturing edible fungus stock by using wood pole |
| CN104718969A (en) * | 2013-12-18 | 2015-06-24 | 于汇 | Production technology and using method for edible mushroom branch mother seeds |
| CN104285677A (en) * | 2014-10-30 | 2015-01-21 | 武汉岁岁丰农业科技开发有限公司 | Method for making edible mushroom pegwood strain |
| CN105237139A (en) * | 2015-08-29 | 2016-01-13 | 安远县天华现代农业有限责任公司 | Preparation and culturing method of tree branch bacterial strain of pleurotus eryngii and hypsizygus marmoreus |
| CN108739047A (en) * | 2018-05-10 | 2018-11-06 | 山东恒发食用菌有限公司 | A kind of production of Pleurotus nebrodensis tree fungus and breeding method |
| CN109156272A (en) * | 2018-08-31 | 2019-01-08 | 成都市宁升绿康食品有限公司 | Seafood mushroom cultivates tree fungus preparation process |
Non-Patent Citations (3)
| Title |
|---|
| 廖智贤: "竹荪开放式制种方法", 《农业科技通讯》 * |
| 张传忠等: "竹木签菌种的制作与使用", 《食用菌》 * |
| 邹方伦主编: "《贵州特色菌物和珍稀菌类的栽培与驯化研究》", 30 June 2007 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112400608A (en) * | 2020-11-09 | 2021-02-26 | 云南省农业技术推广总站 | Method for culturing and propagating strain after inoculation by utilizing mulberry branches and propagation device |
| CN113973645A (en) * | 2021-10-27 | 2022-01-28 | 贵州省山地资源研究所 | Method for preparing Dictyophora rubrovalvata branch seeds |
| CN115336502A (en) * | 2022-08-31 | 2022-11-15 | 贵州金荪生物科技开发有限责任公司 | Formula and preparation method of cultivation fungus sticks of dictyophora rubrovolvata |
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Application publication date: 20200728 |
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