CN115336502A - Formula and preparation method of cultivation fungus sticks of dictyophora rubrovolvata - Google Patents
Formula and preparation method of cultivation fungus sticks of dictyophora rubrovolvata Download PDFInfo
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- 230000003203 everyday effect Effects 0.000 claims description 5
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- 241000475042 Stolonifera Species 0.000 description 1
- RDXARWSSOJYNLI-UHFFFAOYSA-N [P].[K] Chemical compound [P].[K] RDXARWSSOJYNLI-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a dictyophora rubrovolvata cultivation bacteria stick formula which comprises the following raw materials in parts by weight: 70 to 72 parts of wood chips, 8 to 10 parts of bamboo powder, 10 to 12 parts of wheat bran, 3 parts of corn flour, 2 parts of soybean flour, 1 part of white sugar and KH 2 PO 4 0.1 to 0.2 part by weight of MgSO 4 0.03 to 0.05 part by weight, and 1 to 1.5 parts by weight of gypsum powder. A method for preparing Dictyophora rubrovalvata cultivation fungus stick comprises the following steps; stirring, bagging, sterilizing, cooling, inoculating, and culturing. The invention accelerates the growth of dictyophora rubrovolvata hyphae, can effectively shorten the culture time and increase the yield.
Description
Technical Field
Belongs to the technical field of agricultural biology, in particular to a dictyophora rubrovolvata cultivation fungus stick formula and a preparation method thereof.
Background
Dictyophora rubrovalvata (Vent. Ex pers.)Phallus rubrovolvatus Ji et Lio), belonging to basidiomycota, class of gastromycete, order of phalloideae, family of phalloideae, genus phalloidea, also known as bamboo sheng and bamboo ginseng, are known as "Xueyu Xianzi", "fungi emperor", "flowers of fungi" because the fruiting body has a umbrella-shaped skirt. The dictyophora rubrovolvata fruiting body is formed from pileus,The mushroom skirt, the stipe and the bacterium tray are rich in a large amount of amino acids, proteins, vitamins and other nutritional ingredients. Has effects of invigorating qi, nourishing brain, invigorating qi, nourishing yin, resisting tumor, resisting oxidation, resisting aging, and lowering blood sugar. Dictyophora rubrovolvata is a new species discovered in Mr. Kimura 1976 and successfully trained and planted in 1983, 12 species of Dictyophora rubrovolvata are reported all over the world, 7 species of Dictyophora rubrovolvata are discovered in China, and only 4 species of Dictyophora rubrovolvata, dictyophora induciosa, dictyophora phalloidea stolonifera and Dictyophora echinovolvata are edible. The quality of Dictyophora rubrovalvata belongs to the best quality, and Dictyophora rubrovalvata has sweet wine fragrance in fresh products, thick stipe, thick and large fungus skirt veins, no paste after long-term cooking, fresh, crisp and tender mouthfeel, delicious taste and rich nutrition, and is a large fungus which can be used as medicine and food. Dictyophora rubrovalvata is a rare edible fungus, hypha growth is slow, the unique biological characteristic is achieved, the formula components of a dictyophora rubrovalvata cultivation fungus stick in the prior art are single, the nutritional requirements for the growth of Dictyophora rubrovalvata hypha cannot be met, the hypha growth is slow after inoculation, the cultivation period is long, and the yield is low after cultivation.
Disclosure of Invention
The invention aims to overcome the defects and provides a dictyophora rubrovolvata cultivation fungus stick formula and a preparation method thereof, wherein the dictyophora rubrovolvata cultivation fungus stick formula can accelerate the growth of dictyophora rubrovolvata hyphae, effectively shorten the cultivation time and increase the yield.
The purpose of the invention and the main technical problem of solving the invention are realized by adopting the following technical scheme:
the invention relates to a cultivation fungus stick formula of dictyophora rubrovolvata, which comprises the following raw materials in parts by weight:
70 to 72 parts of sawdust, 8 to 10 parts of bamboo powder, 10 to 12 parts of wheat bran, 3 parts of corn flour, 2 parts of soybean meal, 1 part of white sugar and KH 2 PO 4 0.1 to 0.2 part by weight of MgSO 4 0.03 to 0.05 portion of gypsum powder and 1 to 1.5 portions of gypsum powder.
The wood chips are fresh wood chips and do not mildew, the water content is 30-40%, and the particle size of the wood chips is 0.8-1cm.
The bamboo powder is free from mildew, has a water content of 60-70%, is in a sawdust shape, and has a length of 0.3-0.5 cm and a width of 0.1-0.2cm.
The wheat bran, the corn flour and the soybean flour are not mildewed, the water content is 13-15%, the particle size of the wheat bran is 0.2-0.3cm, and the corn flour and the soybean flour are all in powder form.
The sawdust is birch and gao sawdust, cherry sawdust and alder sawdust.
The water content of the compost is 50-60%.
The invention discloses a method for preparing a Dictyophora rubrovalvata cultivation fungus stick, which comprises the following steps;
(1) Stirring: the preparation method comprises accurately weighing the main materials and the auxiliary materials according to the proportion of the formula, repeatedly stirring the main materials including sawdust, bamboo powder, wheat bran, corn flour, soybean powder and gypsum powder according to the material-water ratio of 1: 1.2, and mixing the other auxiliary materials including white sugar and KH 2 PO 4 、MgSO 4 Sequentially dissolving the materials in water, adding 1ppm of triacontanol, fully dissolving the materials to obtain auxiliary material mixed water, finally uniformly stirring the main material mixed water and the auxiliary material mixed water, kneading the materials into a cluster by hand, and dispersing the cluster by light release to obtain the culture material with the pH of 6 to 6.5.
(2) Bagging: and (2) putting the wet mixture obtained in the step (1) into a high-pressure high-density polyethylene bag, inserting a punching rod into one end of the fungus bag, and filling the fungus bag with the wet mixture, wherein the fungus bag must be tightly packed when filled, and the fungus bag is suitable for being held by a hand without finger dents, so that the Dictyophora rubrovolvata fungus stick to be sterilized is obtained.
(3) And (3) sterilization: it is sterilized on the same day by autoclaving at 121 deg.C under 0.135MPa for 3 hr.
(4) And (3) cooling: and (4) timely transferring the sterilized fungus stick cultivation bags obtained in the step (3) to a forced-ventilated cooling room, and inoculating when the cultivation bags are cooled to room temperature.
(5) Inoculation: inoculation is completed within 24 hours and is carried out in an inoculation box or an ultra-clean workbench; the inoculation method comprises wiping hands and inoculating tweezers with sterilized alcohol, taking out the perforating stick from the inoculating end of the stick, inoculating the solid spawn blocks into the holes with the tweezers, filling the whole holes, and sealing with sterilized sponge.
(6) Spawn running culture: and (3) transferring the inoculated cultivation bag into a culture room for cultivation and spawn running, controlling the temperature in a spawn running period to be kept at 24-26 ℃ at room temperature, keeping the temperature dry, and keeping the temperature at normal natural humidity, wherein the cultivation is required to be carried out in a dark environment, ventilating for 30-60min every day, checking the spawn germination and pollution conditions after inoculation for 7 days, timely cleaning the spawn sticks which are not germinated and polluted, cleaning for 2-3 times in the whole spawn running process, and obtaining the dictyophora rubrovolvata spawns to be cultivated after full hyphae grows.
The punching rod is 2cm in diameter, 16cm in length, provided with a hole at the tip and filled with non-degreased cotton.
The high-pressure high-density polyethylene bag has the dimensions of 160cm multiplied by 35cm multiplied by 0.65cc, and the weight after bagging is 1 plus or minus 0.05kg.
The seal is a 4.5cm square sponge.
Compared with the prior art, the invention has obvious beneficial effects, and the technical scheme can be seen as follows: according to the biological characteristics of Dictyophora rubrovalvata mycelium, local wood such as Cinnamomum betulinum, fruit trees and fast-growing wood are selected, high-efficiency phosphorus-potassium compound fertilizer is added as a nutritional agent, and MgSO (MgSO) is added 4 The mineral elements and the triacontanol plant growth regulator are scientifically adjusted and proportioned, so that the growth of hyphae can be effectively promoted, the feeding speed is increased, the growth of mushrooms and fruit bodies is accelerated, and the yield and the biotransformation rate are improved. The culture material has balanced nutrition, and the prepared fungus stick has rapid germination, rapid growth speed and short culture period after inoculation, is suitable for the growth of dictyophora rubrovolvata hyphae, shortens the culture period of the fungus stick for dictyophora rubrovolvata cultivation to a certain extent, and can improve the economic benefit, see table 1;
| control of | Hypha growth rate mm/d | Number of days of full bag/d | Yield: jin/square meter | Number of fruiting/stubble | Biotransformation efficiency% |
| Tradition of | 0.78 | 133 | 2.4 | 1 | 22.85 |
| The invention | 1.0179 | 122 | 4.35 | 2 | 41.42 |
As can be seen from the table 1, the growth speed of hyphae using the cultivation method disclosed by the invention reaches 1.0179mm/d, the bag filling time is 122 days, the hyphae grows full 11 days in advance compared with the traditional formula, the hyphae germinates fast, the fungus age is regular, 400 square meters are effectively planted per mu, 6000 rods can be planted according to 15 rod/square meter planting calculation, the yield of dictyophora rubrovolvata can reach 4.35 kilo/square meter, the yield per mu is 1680 kilo of dictyophora phalloidea, the biological conversion rate is improved by more than 18.57%, 2 batches of mushrooms can be harvested, and the economic benefit is remarkable. Realizes the acceleration of the growth of dictyophora rubrovolvata hyphae, can effectively shorten the culture time and increase the yield.
Detailed Description
The technical means of the present invention will be described in further detail with reference to specific embodiments.
Example 1
The formula of the cultivation fungus stick of Dictyophora rubrovalvata comprises the following raw materials in parts by weight:
70 parts of wood dust and bamboo8 parts of flour, 10 parts of wheat bran, 3 parts of corn flour, 2 parts of soybean flour, 1 part of white sugar and KH 2 PO 4 0.1 part of MgSO 2 4 0.03 part of gypsum powder and 1 part of gypsum powder.
A method for preparing Dictyophora rubrovalvata cultivation fungus stick comprises the following steps;
(1) Stirring: the preparation method comprises accurately weighing the main materials and the auxiliary materials according to the proportion of the formula, repeatedly stirring the main materials including sawdust, bamboo powder, wheat bran, corn flour, soybean powder and gypsum powder according to the material-water ratio of 1: 1.2, and mixing the other auxiliary materials including white sugar and KH 2 PO 4 、MgSO 4 Sequentially dissolving in water, adding 1ppm of triacontanol, fully dissolving to obtain auxiliary material mixed water, stirring the main material and auxiliary material mixed water uniformly, kneading into a dough by hand, and dispersing by light weight to obtain the culture material with pH 6.
(2) Bagging: and (2) filling the compost obtained in the step (1) into a high-pressure high-density polyethylene bag, inserting a punching rod into one end of the fungus bag, filling the fungus bag, wherein the fungus bag must be tightly packed in the filling process, and the fungus bag is suitable for being held by a hand without finger dents to obtain the Dictyophora rubrovolvata fungus stick to be sterilized.
(3) And (3) sterilization: it is sterilized by autoclaving at 121 deg.C under 0.135Mpa for 3 hr.
(4) And (3) cooling: and (4) timely transferring the sterilized bacteria stick cultivation bags obtained in the step (3) to a forced-ventilated cooling room, and inoculating when the cultivation bags are cooled to room temperature.
(5) Inoculation: inoculation needs to be completed within 24 hours, and the inoculation is carried out in an inoculation box or an ultra-clean workbench; the inoculation method comprises wiping hands and inoculating tweezers with sterilized alcohol, taking out the perforating stick from the inoculating end of the fungus stick, inoculating the solid fungus blocks into the holes with the tweezers, filling the holes, and sealing with sterilized sponge.
(6) Spawn running culture: and (3) transferring the inoculated cultivation bag into a cultivation room for cultivation and spawn running, controlling the temperature at room temperature to be kept at 24 ℃ in a spawn running period, keeping the temperature dry, and keeping the temperature at normal natural humidity, wherein the cultivation is required to be carried out in a light-proof environment, ventilating for 30min every day, checking spawn germination and pollution conditions 7 days after inoculation, timely cleaning the spawn sticks which are not germinated and polluted, cleaning for 2 times in the whole spawn running process, and obtaining the dictyophora rubrovolvata spawn sticks to be cultivated after full hypha growth.
(7) Sterilizing soil, removing fungus bags, covering soil for cultivation, covering soil of about 5cm in shape, forming micro arc shape, and covering pine needles with thickness of about 2cm, wherein the soil humidity is maintained at 50%, the air temperature is 20 deg.C, and the humidity is 70%.
(8) Harvesting: and (3) cutting off the thalli from the bottom of the thalli by using a knife, directly pulling and damaging the thalli, peeling off the thalli after picking, slightly rotating until pileus and stipe fall off, and separately placing the pileus and the thalli.
Example 2
The formula of the cultivation fungus stick of Dictyophora rubrovalvata comprises the following raw materials in parts by weight:
71 parts of wood chips, 9 parts of bamboo powder, 11 parts of wheat bran, 3 parts of corn flour, 2 parts of soybean meal, 1 part of white sugar and KH 2 PO 4 0.15 part of MgSO (MgSO) 4 0.04 part of gypsum powder and 1.25 parts of gypsum powder.
A method for preparing Dictyophora rubrovalvata cultivation fungus stick comprises the following steps;
(1) Stirring: the preparation method comprises accurately weighing the main materials and the auxiliary materials according to the proportion of the formula, repeatedly stirring the main materials including sawdust, bamboo powder, wheat bran, corn flour, soybean powder and gypsum powder according to the material-water ratio of 1: 1.2, and mixing the other auxiliary materials including white sugar and KH 2 PO 4 、MgSO 4 Sequentially dissolving in water, adding 1ppm of triacontanol, fully dissolving to obtain auxiliary material mixed water, stirring the main material and auxiliary material mixed water uniformly, kneading into a dough by hand, and dispersing by light weight to obtain the culture material with pH of 6.3.
(2) Bagging: and (2) filling the compost obtained in the step (1) into a high-pressure high-density polyethylene bag, inserting a punching rod into one end of the fungus bag, filling the fungus bag tightly, and holding the fungus bag by hand without finger dents to obtain the dictyophora rubrovolvata fungus rod to be sterilized.
(3) And (3) sterilization: it is sterilized on the same day by autoclaving at 121 deg.C under 0.135MPa for 3 hr.
(4) And (3) cooling: and (4) timely transferring the sterilized fungus stick cultivation bags obtained in the step (3) to a forced-ventilated cooling room, and inoculating when the cultivation bags are cooled to room temperature.
(5) Inoculation: inoculation is completed within 24 hours and is carried out in an inoculation box or an ultra-clean workbench; the inoculation method comprises wiping hands and inoculating tweezers with sterilized alcohol, taking out the perforating stick from the inoculating end of the fungus stick, inoculating the solid fungus blocks into the holes with the tweezers, filling the holes, and sealing with sterilized sponge.
(6) Spawn running culture: and (3) transferring the inoculated cultivation bag into a cultivation room for cultivation and spawn running, controlling the temperature at room temperature to be kept at 25 ℃ in a spawn running period, keeping the temperature dry, and keeping the temperature at normal natural humidity, wherein cultivation is required to be carried out in a light-proof environment, ventilation is carried out for 45min every day, after inoculation is carried out for 7 days, the spawn germination and pollution conditions are checked, the spawn sticks which are not germinated and polluted are cleaned in time, the whole spawn running process needs to be cleaned for 2 times, and the spawns overgrow to obtain the dictyophora rubrovolvata spawns sticks to be cultivated.
(7) Sterilizing the soil, removing the fungus bags, covering soil for cultivation, covering soil of about 5cm in shape, forming micro arc shape, and covering pine needles with a thickness of about 2cm, wherein the soil humidity is kept at 55%, the air temperature is 203 ℃ and the humidity is 75% under management.
(8) Harvesting: and (3) cutting off the thalli from the bottom of the thalli by using a knife, directly pulling and damaging the thalli, peeling off the thalli after picking, slightly rotating until pileus and stipe fall off, and separately placing the pileus and the thalli.
Example 3
The formula of the cultivation fungus stick of Dictyophora rubrovalvata comprises the following raw materials in parts by weight:
72 parts of wood chips, 10 parts of bamboo powder, 12 parts of wheat bran, 3 parts of corn flour, 2 parts of soybean meal, 1 part of white sugar and KH 2 PO 4 0.2 part of MgSO 2 4 0.05 part of gypsum powder and 1.5 parts of gypsum powder.
A method for preparing Dictyophora rubrovalvata cultivation fungus stick comprises the following steps;
(1) Stirring: the preparation method comprises weighing the main materials and the auxiliary materials according to the proportion of the formula, and mixing the main materials including wood dust, bamboo powder, and water according to the material-water ratio of 1: 1.2,Mixing testa Tritici, semen Maydis powder, semen glycines powder and Gypsum Fibrosum powder, stirring, adding white sugar and KH 2 PO 4 、MgSO 4 Sequentially dissolving in water, adding 1ppm of triacontanol, fully dissolving to obtain auxiliary material mixed water, stirring the main material and auxiliary material mixed water uniformly, kneading into a dough by hand, and dispersing by light weight to obtain the culture material with pH of 6.5.
(2) Bagging: and (2) filling the compost obtained in the step (1) into a high-pressure high-density polyethylene bag, inserting a punching rod into one end of the fungus bag, filling the fungus bag, wherein the fungus bag must be tightly packed in the filling process, and the fungus bag is suitable for being held by a hand without finger dents to obtain the Dictyophora rubrovolvata fungus stick to be sterilized.
(3) And (3) sterilization: it is sterilized on the same day by autoclaving at 121 deg.C under 0.135MPa for 3 hr.
(4) And (3) cooling: and (4) timely transferring the sterilized fungus stick cultivation bags obtained in the step (3) to a forced-ventilated cooling room, and inoculating when the cultivation bags are cooled to room temperature.
(5) Inoculation: inoculation is completed within 24 hours and is carried out in an inoculation box or an ultra-clean workbench; the inoculation method comprises wiping hands and inoculating tweezers with sterilized alcohol, taking out the perforating stick from the inoculating end of the fungus stick, inoculating the solid fungus blocks into the holes with the tweezers, filling the holes, and sealing with sterilized sponge.
(6) Spawn running culture: and transferring the inoculated cultivation bag into a cultivation room for cultivation and spawn running, controlling the temperature in a spawn running period to be 26 ℃ at room temperature, keeping the temperature dry, keeping the temperature at the normal natural humidity, requiring cultivation in a light-proof environment, ventilating for 60min every day, checking spawn germination and pollution conditions 7 days after inoculation, timely cleaning un-germinated and polluted mushroom sticks, cleaning for 3 times in the whole spawn running process, and obtaining the dictyophora rubrovolvata mushroom sticks to be cultivated after hyphae overgrows.
(7) Sterilizing soil, removing fungus bags, covering soil for cultivation, covering soil of about 6cm in shape, forming micro arc shape, and covering pine needles with thickness of about 2cm, wherein the soil humidity is maintained at 60%, air temperature is 25 deg.C, and humidity is 80%.
(8) Harvesting: and cutting off the fungus cables from the bottom of the fungus tray by using a knife, directly pulling and damaging the fungus cables, peeling off the fungus tray after picking, slightly rotating until the pileus and the stipe fall off, and separately placing the stipe and the pileus.
While the foregoing is directed to the preferred embodiment of the present invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow.
Claims (10)
1. The formula of the cultivation fungus stick of Dictyophora rubrovalvata comprises the following raw materials in parts by weight:
70 to 72 parts of wood chips, 8 to 10 parts of bamboo powder, 10 to 12 parts of wheat bran, 3 parts of corn flour, 2 parts of soybean flour, 1 part of white sugar and KH 2 PO 4 0.1 to 0.2 part by weight of MgSO 4 0.03 to 0.05 portion of gypsum powder and 1 to 1.5 portions of gypsum powder.
2. The dictyophora rubrovolvata cultivation fungus stick formula according to claim 1, wherein the wood chips are fresh wood chips, do not mildew, have a water content of 30-40% and have a particle size of 0.8-1cm.
3. The cultivation fungus stick formula of dictyophora rubrovolvata as claimed in claim 1, wherein the bamboo powder is free of mildew, has a water content of 60-70%, is in a saw-dust shape, and has a length of 0.3-0.5 cm and a width of 0.1-0.2cm.
4. The Dictyophora rubrovalvata cultivation fungus stick formula according to claim 1, wherein the wheat bran, the corn flour and the soybean flour are not mildewed, the water content is 13 to 15 percent, the particle size of the wheat bran is 0.2 to 0.3cm, and the corn flour and the soybean flour are in powder form.
5. The dictyophora rubrovolvata cultivation stick formula according to claim 1, characterized in that the wood chips are withered and withered wood chips, cherry wood chips and alder wood chips.
6. The cultivation fungus stick formula of dictyophora rubrovolvata as claimed in claim 1, characterized in that the water content of the compost is 50-60%.
7. A method for preparing Dictyophora rubrovalvata cultivation fungus stick comprises the following steps;
(1) Stirring: the preparation method comprises accurately weighing the main materials and the auxiliary materials according to the proportion of the formula, repeatedly stirring the main materials including sawdust, bamboo powder, wheat bran, corn flour, soybean powder and gypsum powder according to the material-water ratio of 1: 1.2, and mixing the other auxiliary materials including white sugar and KH 2 PO 4 、MgSO 4 Sequentially dissolving the materials in water, adding 1ppm of triacontanol, fully dissolving the materials to obtain auxiliary material mixed water, finally uniformly stirring the main material mixed water and the auxiliary material mixed water, kneading the materials into a cluster by hands, and dispersing the cluster by light release to obtain a culture material with the pH value of 6 to 6.5;
(2) Bagging: filling the compost obtained in the step (1) into a high-pressure high-density polyethylene bag, inserting a punching rod into one end of the fungus bag, filling the fungus bag, and tightly filling the fungus bag in a filling process, wherein the fungus bag is suitable for being held by a hand without finger recession to obtain the dictyophora rubrovolvata fungus rod to be sterilized;
(3) And (3) sterilization: sterilizing the strain in the same day by high pressure steam sterilization at 121 deg.C under 0.135Mpa for 3 hr;
(4) And (3) cooling: transferring the sterilized fungus stick cultivation bags obtained in the step (3) to a forced-ventilated cooling room in time, and inoculating when the cultivation bags are cooled to room temperature;
(5) Inoculation: inoculation needs to be completed within 24 hours, and the inoculation is carried out in an inoculation box or an ultra-clean workbench; the inoculation method comprises wiping hands and inoculating tweezers with sterilized alcohol, taking out the perforating stick from the inoculation end of the fungus stick, inoculating the solid fungus blocks into the holes with the tweezers, filling the whole holes, and sealing with sterilized sponge;
(6) Spawn running culture: and (3) transferring the inoculated cultivation bag into a culture room for cultivation and fungus growing, controlling the temperature in a fungus growing period to be kept at 24-26 ℃ at room temperature, keeping the temperature dry, and keeping the general natural humidity, wherein the cultivation is required to be carried out in a dark environment, ventilating for 30-60min every day, checking the germination and pollution conditions of strains after inoculation for 7 days, timely cleaning the fungus sticks without germination and pollution, cleaning for 2-3 times in the whole fungus growing process, and obtaining the dictyophora rubrovolvata fungus sticks to be cultivated after the hyphae overgrows.
8. The method of claim 7, wherein the perforated stick is 2cm in diameter, 16cm in length, has a hole at its tip, and is packed with non-absorbent cotton.
9. The method for preparing a dictyophora rubrovolvata cultivation fungus stick as claimed in claim 7, wherein the size of the high-pressure high-density polyethylene bag is 160cm x 35cm x 0.65cc, and the weight of the bag is 1 plus or minus 0.05kg.
10. The method for preparing Dictyophora rubrovalvata cultivation mushroom stick according to claim 7, wherein the seal is a square sponge of 4.5 cm.
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