CN104938213A - Pleurotuseryngii pollution-free cultivation method - Google Patents
Pleurotuseryngii pollution-free cultivation method Download PDFInfo
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- CN104938213A CN104938213A CN201510347849.0A CN201510347849A CN104938213A CN 104938213 A CN104938213 A CN 104938213A CN 201510347849 A CN201510347849 A CN 201510347849A CN 104938213 A CN104938213 A CN 104938213A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Pest Control & Pesticides (AREA)
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Abstract
The invention discloses a pleurotuseryngii pollution-free cultivation method and belongs to the technical field of edible mushroom cultivation. The pleurotuseryngii pollution-free cultivation method comprises the following steps of cultivation material preparation, bottling and sterilization, inoculation, spawn running, primordia hastening, fruiting management and harvest. Pleurotuseryngii produced by the cultivation method is rich in nutrition, big in size, good in quality, good in taste and high in production volume, and the economic benefit of farmer households is increased.
Description
Technical field
The present invention relates to the culture technique of edible mushroom, particularly a kind of Xingbao mushroom method for non-polluted cultivation.
Background technology
Xingbao mushroom has another name called pleurotus eryngii, belongs to loose Zoopagales Pleurotaceae Pleurotus.Xingbao mushroom is that exploitation cultivation successfully integrates edible, medicinal, the Rare edible fungus new varieties of dietotherapy.Mushroom body has almond flavor, and meat is plump, and mouthfeel is fresh and tender, fragrant taste, nutritious, can cook tens road delicious foods.Xingbao mushroom is nutritious, and Xingbao mushroom is rich in the amino acid/11 7 kinds that total amount is 15.85%, and wherein the amino acid content of needed by human is 6.65%; In the dry mushroom of Xingbao mushroom, protein content is 20%, and crude fiber content is 13.28%, and crude fat content is 3.5%, is also rich in several mineral materials.Also have reducing blood lipid, norcholesterol, promotion gastro-intestinal digestion, strengthen body immunity, prevent the effects such as cardiovascular disease, pole is liked by people, and the market price is higher than flat mushroom 3 ~ 5 times.
Current Xingbao mushroom is mainly produced in a conventional manner, yields poorly and unstable, produces the impact of cultivation method by season, facility, environmental condition, nutrient imbalance causes bacterial classification survival rate low, mycelia is easily aging, causes bacterial classification to make a variation, and affects the growth of later stage Xingbao mushroom, even cause the underproduction of Xingbao mushroom, degradation serious consequence under quality, and the many not science of the batching of the planting material of most of Xingbao mushroom, trophic structure are unreasonable, cause the mycelia growing of pleurotus eryngii slow, and the cost of planting material is high, economic benefit is not good.
Summary of the invention
The object of the invention is to: for above-mentioned Problems existing, provide a kind of Xingbao mushroom method for non-polluted cultivation, the Xingbao mushroom of production is nutritious, individual greatly, quality is good, mouthfeel good, and output is high, increases the economic benefit at plantation family.
The technical solution used in the present invention is as follows:
A kind of Xingbao mushroom method for non-polluted cultivation, comprises the following steps:
1) planting material preparation: get out every raw material by the formula of planting material, the raw material chosen is fresh, dry, nothing is gone mouldy, and various material mixing is mixed thoroughly, fermentation 16 ~ 20h, after fermentation completes, then the stirring that adds water, ensure that planting material water content is 62% ~ 68%, pH value is 7.8 ~ 8.2;
2) to bottle sterilizing: by step 1) planting material for preparing puts into culture bottle, with film, culture bottle is sealed, and then by culture bottle middle sterilizing 2 ~ 2.5h under the pressure of 0.1 ~ 0.2MPa, sterilising temp is 110 ~ 120 DEG C, for subsequent use after being cooled to 25 ~ 30 DEG C;
3) inoculate: open film, adopt inoculating tool to implant in the planting material of culture bottle by pleurotus eryngii quel strains by aseptic guidelines; After inoculating, culture bottle moves into culturing room's lucifuge cultivation again, and culturing room's temperature controls at 20 ~ 26 DEG C, and humid control is 65 ~ 70%;
4) bacterium is sent out: within first week, send out bacterium indoor temperature and should control at 25 ~ 27 DEG C; Second week is sent out bacterium indoor temperature and should be controlled at 23 ~ 25 DEG C; The temperature risen for 3rd week should control at 21 ~ 23 DEG C, and 40 ~ 45d mycelia covers with culture bottle; PH value controls 6.8 ~ 7.2;
5) urge flower bud: after mycelia covers with culture bottle, continue cultivation 8 ~ 10d, carry out urging flower bud at 10 ~ 16 DEG C of temperature, culturing room's humidity controls, 90 ~ 95%, to add forced ventilation, and increases scattered light stimulation; Occur white former base through 3 ~ 6d, former base forms mushroom flower bud gradually;
6) management of producing mushroom: when former base is differentiated to form the small mushroom bud of 1 ~ 2cm, management of producing mushroom should be carried out in time, each bottleneck stays 3 ~ 4 mushroom flower buds, in the fruiting stage, pH value is 5.5 ~ 6.5, and culturing room's temperature should remain on 10 ~ 12 DEG C, after bacteria cover diameter grows to 2 ~ 3cm, carry out spray cooling humidification, gather first 2 days, the relative air humidity sending out bacterium room should control 90 ~ 95%;
7) gather: when Xingbao mushroom fruit body grows to more than 5cm, should gather in time; Hold tight stem when gathering to lift, be placed in plastic crate.
As preferred version of the present invention, described composts or fertilisers of cultivating is made up of the raw material of following weight percentage: cotton seed hulls 40 ~ 60%, hardwood crumbs 15 ~ 25%, bagasse 3 ~ 6%, beanstalk 5 ~ 8%, onion powder 2 ~ 5%, dried orange peel 0.6 ~ 1.5%, potassium dihydrogen phosphate 0.2 ~ 0.6%, land plaster 0.8 ~ 1.5%, brown sugar 0.5 ~ 1.0%.
As preferred version of the present invention, the requirement of described increase scattered light is illuminance 600 ~ 1000lux, irradiation time 6 ~ 8h.
In sum, beneficial effect of the present invention:
1, pleurotus eryngii cultivating material scientific matching of the present invention; with the addition of the nutritive element that cotton seed hulls, hardwood crumbs, bagasse, beanstalk, onion powder, dried orange peel, potassium dihydrogen phosphate, land plaster, brown sugar etc. meet woodear growth needs; scientific matching ensures needed for nutrition; avoid overnutrition or oligotrophy; obtained composts or fertilisers of cultivating material is loose, good permeability, mycelial growth have sufficient oxygen; meet the temperature and humidity of Growth of Pleurotus eryngii environment, thus ensure that it well absorbs the nutrition of composts or fertilisers of cultivating.
2, the control of the inventive method temperature, humidity, pH value is not only conducive to the growth of fungus mycelium, is also fruiting phase high yield, stablizes and lay a good foundation; The Xingbao mushroom produced is nutritious, individual greatly, quality is good, mouthfeel good, and output is high, increases the economic benefit at plantation family.
Embodiment
The present invention is not limited to the embodiment of the following stated; the following stated is only preferred embodiment of the present invention; not in order to limit the present invention; all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Below in conjunction with embodiment, the present invention is further described:
Embodiment 1
A kind of Xingbao mushroom method for non-polluted cultivation, comprises the following steps:
1) planting material preparation: get out every raw material by the formula of planting material, the raw material chosen is fresh, dry, nothing is gone mouldy, the mixing of cotton seed hulls 55%, hardwood crumbs 20%, bagasse 5%, beanstalk 6%, onion powder 3%, dried orange peel 0.8%, potassium dihydrogen phosphate 0.5%, land plaster 1.0% and brown sugar 0.8% is mixed thoroughly, fermentation 18h, after fermentation completes, add water stirring again, and ensure that planting material water content is 65%, pH value is 8.0;
2) to bottle sterilizing: by step 1) planting material for preparing puts into culture bottle, with film, culture bottle is sealed, and then by sterilizing 2h during culture bottle is under the pressure of 0.15MPa, sterilising temp is 110 DEG C, for subsequent use after being cooled to 28 DEG C;
3) inoculate: open film, adopt inoculating tool to implant in the planting material of culture bottle by pleurotus eryngii quel strains by aseptic guidelines; After inoculating, culture bottle moves into culturing room's lucifuge cultivation again, and culturing room's temperature controls at 22 DEG C, and humid control is 70%;
4) bacterium is sent out: within first week, send out bacterium indoor temperature and should control at 26 DEG C; Second week is sent out bacterium indoor temperature and should be controlled at 24 DEG C; The temperature risen for 3rd week should control at 22 DEG C, and 40d mycelia covers with culture bottle; PH value controls 6.8;
5) urge flower bud: after mycelia covers with culture bottle, continue to cultivate 10d, carry out urging flower bud at 15 DEG C of temperature, culturing room's humidity controls, 90%, to add forced ventilation, and increases scattered light stimulation; Occur white former base through 5d, former base forms mushroom flower bud gradually;
6) management of producing mushroom: when former base is differentiated to form the small mushroom bud of 2cm, management of producing mushroom should be carried out in time, each bottleneck stays 4 mushroom flower buds, in the fruiting stage, pH value is 5.8, and culturing room's temperature should remain on 10 DEG C, after bacteria cover diameter grows to 3cm, carry out spray cooling humidification, gather first 2 days, the relative air humidity sending out bacterium room should control 95%;
7) gather: when Xingbao mushroom fruit body grows to more than 5cm, should gather in time; Hold tight stem when gathering to lift, be placed in plastic crate.
Embodiment 2
A kind of Xingbao mushroom method for non-polluted cultivation, comprises the following steps:
1) planting material preparation: get out every raw material by the formula of planting material, the raw material chosen is fresh, dry, nothing is gone mouldy, the mixing of cotton seed hulls 60%, hardwood crumbs 15%, bagasse 3%, beanstalk 8%, onion powder 5%, dried orange peel 1.5%, potassium dihydrogen phosphate 0.6%, land plaster 1.5% and brown sugar 1.0% is mixed thoroughly, fermentation 20h, after fermentation completes, add water stirring again, and ensure that planting material water content is 68%, pH value is 8.2;
2) to bottle sterilizing: by step 1) planting material for preparing puts into culture bottle, with film, culture bottle is sealed, and then by sterilizing 2.5h during culture bottle is under the pressure of 0.2MPa, sterilising temp is 120 DEG C, for subsequent use after being cooled to 30 DEG C;
3) inoculate: open film, adopt inoculating tool to implant in the planting material of culture bottle by pleurotus eryngii quel strains by aseptic guidelines; After inoculating, culture bottle moves into culturing room's lucifuge cultivation again, and culturing room's temperature controls at 26 DEG C, and humid control is 70%;
4) bacterium is sent out: within first week, send out bacterium indoor temperature and should control at 25 DEG C; Second week is sent out bacterium indoor temperature and should be controlled at 23 DEG C; The temperature risen for 3rd week should control at 21 DEG C, and 40 ~ 45d mycelia covers with culture bottle; PH value controls 7.2;
5) urge flower bud: after mycelia covers with culture bottle, continue to cultivate 10d, carry out urging flower bud at 16 DEG C of temperature, culturing room's humidity controls, 95%, to add forced ventilation, and increases scattered light stimulation; Occur white former base through 6d, former base forms mushroom flower bud gradually;
6) management of producing mushroom: when former base is differentiated to form the small mushroom bud of 2cm, management of producing mushroom should be carried out in time, each bottleneck stays 4 mushroom flower buds, in the fruiting stage, pH value is 6.5, and culturing room's temperature should remain on 12 DEG C, after bacteria cover diameter grows to 3cm, carry out spray cooling humidification, gather first 2 days, the relative air humidity sending out bacterium room should control 95%;
7) gather: when Xingbao mushroom fruit body grows to more than 5cm, should gather in time; Hold tight stem when gathering to lift, be placed in plastic crate.
Embodiment 3
A kind of Xingbao mushroom method for non-polluted cultivation, comprises the following steps:
1) planting material preparation: get out every raw material by the formula of planting material, the raw material chosen is fresh, dry, nothing is gone mouldy, the mixing of cotton seed hulls 40%, hardwood crumbs 25%, bagasse 6%, beanstalk 5%, onion powder 5%, dried orange peel 1.5%, potassium dihydrogen phosphate 0.6%, land plaster 1.5% and brown sugar 1.0% is mixed thoroughly, fermentation 16h, after fermentation completes, add water stirring again, and ensure that planting material water content is 62%, pH value is 7.8;
2) to bottle sterilizing: by step 1) planting material for preparing puts into culture bottle, with film, culture bottle is sealed, and then by sterilizing 2h during culture bottle is under the pressure of 0.1MPa, sterilising temp is 110 DEG C, for subsequent use after being cooled to 25 DEG C;
3) inoculate: open film, adopt inoculating tool to implant in the planting material of culture bottle by pleurotus eryngii quel strains by aseptic guidelines; After inoculating, culture bottle moves into culturing room's lucifuge cultivation again, and culturing room's temperature controls at 20 DEG C, and humid control is 65%;
4) bacterium is sent out: within first week, send out bacterium indoor temperature and should control at 25 DEG C; Second week is sent out bacterium indoor temperature and should be controlled at 23 DEG C; The temperature risen for 3rd week should control at 21 DEG C, and 40d mycelia covers with culture bottle; PH value controls 6.8;
5) urge flower bud: after mycelia covers with culture bottle, continue to cultivate 8d, carry out urging flower bud at 10 DEG C of temperature, culturing room's humidity controls, 90%, to add forced ventilation, and increases scattered light stimulation; Occur white former base through 3d, former base forms mushroom flower bud gradually;
6) management of producing mushroom: when former base is differentiated to form the small mushroom bud of 1cm, management of producing mushroom should be carried out in time, each bottleneck stays 3 mushroom flower buds, in the fruiting stage, pH value is 5.5, and culturing room's temperature should remain on 10 ~ 12 DEG C, after bacteria cover diameter grows to 2cm, carry out spray cooling humidification, gather first 2 days, the relative air humidity sending out bacterium room should control 90%;
7) gather: when Xingbao mushroom fruit body grows to more than 5cm, should gather in time; Hold tight stem when gathering to lift, be placed in plastic crate.
Mensuration to the Xingbao mushroom nutrient component of above-described embodiment 1-3:
After testing, every 100 grams of Xingbao mushroom fruit bodys about contain 21.85 grams, protein, 1.83 grams, fat, 57.35 grams, carbohydrate, raw fiber 6.52 grams, reducing sugar 2.27g, total reducing sugar 35.87g, water soluble ingredient 66.87g, mannitol 2.18g, free amino acid 2.41g.
As from the foregoing, the Xingbao mushroom that method of the present invention is produced, nutritious, environmental protection is nuisanceless, individual large, quality is good, mouthfeel good, output is high.
Claims (3)
1. an Xingbao mushroom method for non-polluted cultivation, is characterized in that comprising the following steps:
1) planting material preparation: get out every raw material by the formula of planting material, the raw material chosen is fresh, dry, nothing is gone mouldy, and various material mixing is mixed thoroughly, fermentation 16 ~ 20h, after fermentation completes, then the stirring that adds water, ensure that planting material water content is 62% ~ 68%, pH value is 7.8 ~ 8.2;
2) to bottle sterilizing: by step 1) planting material for preparing puts into culture bottle, with film, culture bottle is sealed, and then by culture bottle middle sterilizing 2 ~ 2.5h under the pressure of 0.1 ~ 0.2MPa, sterilising temp is 110 ~ 120 DEG C, for subsequent use after being cooled to 25 ~ 30 DEG C;
3) inoculate: open film, adopt inoculating tool to implant in the planting material of culture bottle by pleurotus eryngii quel strains by aseptic guidelines; After inoculating, culture bottle moves into culturing room's lucifuge cultivation again, and culturing room's temperature controls at 20 ~ 26 DEG C, and humid control is 65 ~ 70%;
4) bacterium is sent out: within first week, send out bacterium indoor temperature and should control at 25 ~ 27 DEG C; Second week is sent out bacterium indoor temperature and should be controlled at 23 ~ 25 DEG C; The temperature risen for 3rd week should control at 21 ~ 23 DEG C, and 40 ~ 45d mycelia covers with culture bottle; PH value controls 6.8 ~ 7.2;
5) urge flower bud: after mycelia covers with culture bottle, continue cultivation 8 ~ 10d, carry out urging flower bud at 10 ~ 16 DEG C of temperature, culturing room's humidity controls, 90 ~ 95%, to add forced ventilation, and increases scattered light stimulation; Occur white former base through 3 ~ 6d, former base forms mushroom flower bud gradually;
6) management of producing mushroom: when former base is differentiated to form the small mushroom bud of 1 ~ 2cm, management of producing mushroom should be carried out in time, each bottleneck stays 3 ~ 4 mushroom flower buds, in the fruiting stage, pH value is 5.5 ~ 6.5, and culturing room's temperature should remain on 10 ~ 12 DEG C, after bacteria cover diameter grows to 2 ~ 3cm, carry out spray cooling humidification, gather first 2 days, the relative air humidity sending out bacterium room should control 90 ~ 95%;
7) gather: when Xingbao mushroom fruit body grows to more than 5cm, should gather in time; Hold tight stem when gathering to lift, be placed in plastic crate.
2. a kind of Xingbao mushroom method for non-polluted cultivation according to claim 1, is characterized in that described composts or fertilisers of cultivating is made up of the raw material of following weight percentage: cotton seed hulls 40 ~ 60%, hardwood crumbs 15 ~ 25%, bagasse 3 ~ 6%, beanstalk 5 ~ 8%, onion powder 2 ~ 5%, dried orange peel 0.6 ~ 1.5%, potassium dihydrogen phosphate 0.2 ~ 0.6%, land plaster 0.8 ~ 1.5%, brown sugar 0.5 ~ 1.0%.
3. a kind of Xingbao mushroom method for non-polluted cultivation according to claim 1, is characterized in that: the requirement of described increase scattered light is illuminance 600 ~ 1000lux, irradiation time 6 ~ 8h.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105379556A (en) * | 2015-10-28 | 2016-03-09 | 江苏久禾生物科技发展有限公司 | Industrial pleurotus erygii cultivation method adopting temperature-controlled fruiting management technique |
| CN105503443A (en) * | 2016-01-15 | 2016-04-20 | 西南大学 | Flue-cured tobacco straw substitute cultivation pleurotus eryngii substrate and preparation method thereof |
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|---|---|---|---|---|
| JPH07184473A (en) * | 1993-12-28 | 1995-07-25 | Masakichi Matsui | Method for artificially cultivating mushroom of genus pleurotus |
| CN101857494A (en) * | 2010-06-01 | 2010-10-13 | 浙江海洋学院 | Culture medium for pleurotus eryngii and cultivation method of pleurotus eryngii |
| CN101863704A (en) * | 2010-06-21 | 2010-10-20 | 浙江海洋学院 | Pleurotus eryngii culture medium and culture method thereof |
| CN102898191A (en) * | 2011-07-29 | 2013-01-30 | 张娇 | Preparation method for cultivation material of pleurotus eryngii |
| CN103333023A (en) * | 2013-07-05 | 2013-10-02 | 邬金飞 | Pleurotus eryngii cultivation material compatibility and manufacture method of cultivation material |
| CN103408369A (en) * | 2013-08-08 | 2013-11-27 | 邬金飞 | Compatibility and manufacturing method of Pleurotus eryngii cultivation material |
| CN103992177A (en) * | 2014-05-20 | 2014-08-20 | 广西壮族自治区农业科学院植物保护研究所 | High-yield cultivation method for pleurotus eryngii and culture medium therefor |
| CN103999692A (en) * | 2014-06-06 | 2014-08-27 | 江苏久禾生物科技发展有限公司 | Industrial cultivation method of pleurotus eryngii |
| CN104303821A (en) * | 2014-09-15 | 2015-01-28 | 管兴 | Method for cultivating pleurotus eryngii industrially |
-
2015
- 2015-06-23 CN CN201510347849.0A patent/CN104938213A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07184473A (en) * | 1993-12-28 | 1995-07-25 | Masakichi Matsui | Method for artificially cultivating mushroom of genus pleurotus |
| CN101857494A (en) * | 2010-06-01 | 2010-10-13 | 浙江海洋学院 | Culture medium for pleurotus eryngii and cultivation method of pleurotus eryngii |
| CN101863704A (en) * | 2010-06-21 | 2010-10-20 | 浙江海洋学院 | Pleurotus eryngii culture medium and culture method thereof |
| CN102898191A (en) * | 2011-07-29 | 2013-01-30 | 张娇 | Preparation method for cultivation material of pleurotus eryngii |
| CN103333023A (en) * | 2013-07-05 | 2013-10-02 | 邬金飞 | Pleurotus eryngii cultivation material compatibility and manufacture method of cultivation material |
| CN103408369A (en) * | 2013-08-08 | 2013-11-27 | 邬金飞 | Compatibility and manufacturing method of Pleurotus eryngii cultivation material |
| CN103992177A (en) * | 2014-05-20 | 2014-08-20 | 广西壮族自治区农业科学院植物保护研究所 | High-yield cultivation method for pleurotus eryngii and culture medium therefor |
| CN103999692A (en) * | 2014-06-06 | 2014-08-27 | 江苏久禾生物科技发展有限公司 | Industrial cultivation method of pleurotus eryngii |
| CN104303821A (en) * | 2014-09-15 | 2015-01-28 | 管兴 | Method for cultivating pleurotus eryngii industrially |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105379556A (en) * | 2015-10-28 | 2016-03-09 | 江苏久禾生物科技发展有限公司 | Industrial pleurotus erygii cultivation method adopting temperature-controlled fruiting management technique |
| CN105503443A (en) * | 2016-01-15 | 2016-04-20 | 西南大学 | Flue-cured tobacco straw substitute cultivation pleurotus eryngii substrate and preparation method thereof |
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