CN101456769A - Bag-cultivation shiitake chopstick strain raw material formula and preparation process - Google Patents
Bag-cultivation shiitake chopstick strain raw material formula and preparation process Download PDFInfo
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- CN101456769A CN101456769A CNA2007101588487A CN200710158848A CN101456769A CN 101456769 A CN101456769 A CN 101456769A CN A2007101588487 A CNA2007101588487 A CN A2007101588487A CN 200710158848 A CN200710158848 A CN 200710158848A CN 101456769 A CN101456769 A CN 101456769A
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- wooden chopsticks
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- 239000002994 raw material Substances 0.000 title claims abstract description 16
- 240000000599 Lentinula edodes Species 0.000 title abstract description 6
- 235000001715 Lentinula edodes Nutrition 0.000 title abstract description 4
- 238000002360 preparation method Methods 0.000 title description 2
- 238000009472 formulation Methods 0.000 title 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 33
- 239000000463 material Substances 0.000 claims abstract description 19
- 230000001954 sterilising effect Effects 0.000 claims abstract description 18
- 238000004519 manufacturing process Methods 0.000 claims abstract description 15
- 238000012258 culturing Methods 0.000 claims abstract description 13
- 239000010440 gypsum Substances 0.000 claims abstract description 7
- 229910052602 gypsum Inorganic materials 0.000 claims abstract description 7
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 6
- 239000002023 wood Substances 0.000 claims abstract description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 21
- 235000016709 nutrition Nutrition 0.000 claims description 18
- 230000035764 nutrition Effects 0.000 claims description 18
- 238000011081 inoculation Methods 0.000 claims description 9
- 230000000050 nutritive effect Effects 0.000 claims description 8
- 239000004615 ingredient Substances 0.000 claims description 6
- 229910019142 PO4 Inorganic materials 0.000 claims description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 4
- 238000010521 absorption reaction Methods 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- 239000002054 inoculum Substances 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 4
- 239000010452 phosphate Substances 0.000 claims description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 4
- 239000011591 potassium Substances 0.000 claims description 4
- 229910052700 potassium Inorganic materials 0.000 claims description 4
- 238000009423 ventilation Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 241000222519 Agaricus bisporus Species 0.000 claims 5
- 238000000034 method Methods 0.000 abstract description 8
- 241000894007 species Species 0.000 abstract 1
- 230000004083 survival effect Effects 0.000 abstract 1
- 244000251953 Agaricus brunnescens Species 0.000 description 15
- 230000001580 bacterial effect Effects 0.000 description 9
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 239000011121 hardwood Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 235000012204 lemonade/lime carbonate Nutrition 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 235000019691 monocalcium phosphate Nutrition 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
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Abstract
A bag-cultivation shiitake chopstick strain raw material formula and a manufacturing process relate to a bag-cultivation shiitake strain raw material formula and a manufacturing process for manufacturing strains by using wooden chopsticks, and belong to the field of edible mushroom production. The invention provides a bag-cultivated mushroom chopstick strain raw material formula which is rich in source, low in cost and high in survival rate, and provides a production process of the bag-cultivated mushroom chopstick strain which is simple and convenient to operate, high in strain purity and extremely low in pollution rate. The raw material formula of the invention comprises the following main materials and auxiliary materials by weight percent: main materials: 100 pairs of wooden chopsticks/bag; auxiliary materials: 79-88% of wood chips, 11-20% of wheat bran and 1% of gypsum. The making process of the chopstick strains comprises the following steps: preparing raw materials; (2) processing chopsticks; (3) bagging treatment; (4) sterilizing; (5) inoculating and culturing; (6) the mushroom chopstick strains are used for transferring cultivated species.
Description
Technical field:
The present invention relates to a kind of bag-cultivation of shiitake fungus strain raw material formula and utilize wooden chopsticks to make the manufacture craft of bacterial classification, belong to the Edible Fungi field.
Background technology:
Mushroom (Lentinus edodes), another name mushroom, thick mushroom are that one of maximum edible fungus variety is measured in the northern area plantation.The mushroom mycelium vitality is extremely strong, can make full use of matrix nutrition, is applicable to plurality of raw materials, and cotton seed hulls, weed tree sawdust, corn cob etc. all can be decided and be planted raw material; The cultivation auxiliary material mainly contains wheat bran, rice bran, sucrose, lime carbonate, calcium superphosphate etc.In the bacterial classification production process, usually adopt conventional solid spawn inoculation technique and method, common bag-cultivation of shiitake fungus bacterial classification cell age obvious difference, the upper, middle and lower position cell age speed of growth is inconsistent, inner easily implicit assorted bacterium, make to pollute to increase and improve, generally can reach about 20%, culture cycle is long, the growth cycle is 40-50 days, the mycelia vigor descends, and after planting bacterial classification recovers and the material feeding ability, causes producing the failure that the collarium joint is sent out in cultivation.Inoculating process is loaded down with trivial details, and speed is slow, and is time-consuming, influences producer's direct economic benefit.
Summary of the invention:
The present invention is exactly at the problems referred to above, and a kind of bag cultivation champignon chopstick strain raw material formula abundant, that cost is low, surviving rate is high of originating is provided, and the manufacture craft of a kind of easy and simple to handle, bacterial classification purity height, bag cultivation champignon chopstick strain that pollution rate is extremely low is provided.
For achieving the above object, the present invention adopts following technical scheme, and composition of raw materials of the present invention is made up of by following weight percent major ingredient and auxiliary material: major ingredient: 100 pairs/bag of wooden chopsticks; Auxiliary material: wood chip 79-88%, wheat bran 11-20%, gypsum 1%.
The manufacture craft of chopstick strain of the present invention may further comprise the steps:
(1) raw material configuration: major ingredient: 100 pairs/bag of wooden chopsticks; Auxiliary material: wood chip 79-88%, wheat bran 11-20%, gypsum 1%, make nutrition accessory; Nutrition accessory configures the water content to 60% that nutrition accessory is adjusted in the back.
(2) wooden chopsticks are handled: wooden chopsticks are fined away, put into container, press with weight, with 1.0% sugar, 0.2-0.5% potassium primary phosphate and 0.1-0.3% sal epsom, make nutritive medium, wooden chopsticks are soaked 12-24h, or with nutritive medium poach 20-60min, allow the abundant moisture absorption of wooden chopsticks, do not have the dried heart and can pull out;
(3) pack is handled: roll in the nutrition accessory for preparing in the two wooden chopsticks ratios of every bag of 50-100, make wooden chopsticks fully touch nutrition accessory on every side up and down, will interspace between the wooden chopsticks, pack did not highly have wooden chopsticks 2cm, tying then;
(4) sterilization: autoclaving requires 1.5kg/cm
2Under the pressure, sterilization 2-2.5h; Normal-pressure sterilization is under 100 ℃ of conditions of temperature, and 10-12h sterilizes;
(5) inoculation culture: sack is opened female kind of access mushroom particle, and inoculum size is 15%-20%, and temperature is controlled at 23 ± 1 ℃ in the culturing room of immigration cleaning then; Guarantee ventilation, mycelia can be covered with pocket in 25 days-30 days, and champignon chopstick strain is made;
(6) with the champignon chopstick strain cultivar of transferring:
Cultivar after the sterilization is directly put on the culturing rack,, reduced to 15-25 ℃, whole pair of chopstick strain directly inserted in the material, stay 1cm to seal, cultivated 25-35 days, make the cultivating champignon kind when the cultivating bag temperature at culturing room's disinfection together.
Beneficial effect of the present invention:
The present invention compares with conventional mushroom solid spawn manufacture craft, the invention belongs to open inoculation, and easy and simple to handle, infection rate is low; Cell age is unanimous between the higher and lower levels, bacterial classification purity height, and anti-assorted power is strong; Culture cycle is short, fuel saving; Expand numerous index and improve 2～3 times, reduce with kind of a cost.Shorten and cultivate 10-15 days mycelia time, the bacterial classification reguarity is simplified vaccine program, shortens inoculation time.
Description of drawings:
Fig. 1 is a bag cultivation champignon chopstick strain manufacture craft of the present invention.
Embodiment:
Embodiment 1:2500 bag champignon chopstick strain is produced
(1) strain culture-material configuration:
2500 bags of three grades of kinds need 25 bags of chopsticks kinds (100 pairs every bag), and nutrition accessory is considered to be worth doing with 2000g hardwood, the 400g brown shorts, and white sugar 25g, gypsum 25g is made into, and the water content of adjusting nutrition accessory reaches 60%.
(2) wooden chopsticks are handled:
Manufacture per 100 pairs with wooden chopsticks and adorn one bag (every mu or 2500 pairs of wooden chopsticks of every canopy preparation), wooden chopsticks are fined away, wooden chopsticks are put into container, press with weight,, make nutritive medium with 1.0% sugar, 0.2% potassium primary phosphate and 0.1% sal epsom, wooden chopsticks are soaked 12-24h, also available nutritive medium poach 20min allows the abundant moisture absorption of wooden chopsticks, does not have the dried heart and can pull out.
(3) pack is handled: roll in the nutrition accessory for preparing in 100 pairs every bag wooden chopsticks ratios, make wooden chopsticks fully touch nutrition accessory up and down on every side, to interspace between the wooden chopsticks, not only ventilate, also be beneficial to when inoculation bacterial classification and fall in the middle of the wooden chopsticks and a bag end, will at the bottom of bag, spread one deck before the pack and prepare burden, prevent that wooden chopsticks from puncturing bag at the bottom of, pack height not have chopsticks 2cm centimetre to be advisable, then tying.
(4) sterilization: autoclaving requires 1.5kg/cm
2Under the pressure, sterilization 2-2.5h.Normal-pressure sterilization should reach 100 ℃ in temperature, sterilization 10-12h.
(5) inoculation culture: carry out aseptic technique according to a conventional method, sack is opened inserted the female kind of mushroom particle, inoculum size is 15%-20%, and temperature is controlled at 23 ± 1 ℃ in the culturing room of immigration cleaning then.Guarantee ventilation, mycelia both can be covered with pocket in 25 days-30 days, and champignon chopstick strain is made.
(6) connect the cultivar method with champignon chopstick strain
Cultivar after the sterilization is directly put on the culturing rack after taking the dish out of the pot,, when the cultivating bag temperature is reduced to 25 ℃, whole pair of chopstick strain directly inserted in the material, stay 1cm to seal, cultivated 25-35 days, make the cultivating champignon kind with culturing room's disinfection together.
Embodiment 2:5000 bag champignon chopstick strain is produced
(1) strain culture-material configuration:
5000 bags of three grades of kinds need 50 bags of chopsticks kinds (100 pairs every bag), and auxiliary material is considered to be worth doing with 3500g hardwood, the 1300g brown shorts, and white sugar 50g, gypsum 50g is made into, and the water content of adjusting nutrition accessory reaches 60%.
(2) wooden chopsticks are handled:
Manufacture per 100 pairs with wooden chopsticks and adorn one bag, wooden chopsticks are fined away, wooden chopsticks are put into container, press with weight,, make nutritive medium with 0.5% sugar, 0.2% potassium primary phosphate and 0.05% sal epsom, wooden chopsticks are soaked 12-24h, also available nutritive medium poach 20min allows the abundant moisture absorption of wooden chopsticks, does not have the dried heart and can pull out.
(3) pack is handled: roll in the nutrition accessory for preparing in 100 pairs every bag wooden chopsticks ratios, make wooden chopsticks fully touch nutrition accessory up and down on every side, to interspace between the wooden chopsticks, not only ventilate, also be beneficial to when inoculation bacterial classification and fall in the middle of the wooden chopsticks and a bag end, will at the bottom of bag, spread one deck before the pack and prepare burden, prevent that wooden chopsticks from puncturing bag at the bottom of, pack height not have wooden chopsticks 2cm to be advisable, then tying.
(4) sterilization: autoclaving requires 1.5kg/cm
2Under the pressure, sterilization 2-2.5h.Normal-pressure sterilization should reach 100 ℃ of sterilization 10-12h down in temperature.
(5) inoculation culture: carry out aseptic technique according to a conventional method, sack is opened inserted the female kind of mushroom particle, inoculum size is 15%-20%, and temperature is controlled at 23 ± 1 ℃ in the culturing room of immigration cleaning then.Guarantee ventilation, mycelia both can be covered with pocket in 25 days-30 days, and champignon chopstick strain is made.
(6) connect the cultivar method with champignon chopstick strain
Cultivar after the sterilization is directly put on the culturing rack after taking the dish out of the pot,, when the cultivating bag temperature is reduced to 25 ℃, whole pair of chopstick strain directly inserted in the material, stay 1cm to seal, cultivated 25-35 days, make the cultivating champignon kind with culturing room's disinfection together.
Claims (2)
1, bag cultivation champignon chopstick strain raw material formula is characterized in that composition of raw materials is made up of by following weight percent major ingredient and auxiliary material: major ingredient: 100 pairs/bag of wooden chopsticks; Auxiliary material: wood chip 79-88%, wheat bran 11-20%, gypsum 1%.
2, bag cultivation champignon chopstick strain manufacture craft is characterized in that may further comprise the steps:
(1) raw material configuration: major ingredient: 100 pairs/bag of wooden chopsticks; Auxiliary material: wood chip 79-88%, wheat bran 11-20%, gypsum 1%, make nutrition accessory; Nutrition accessory configures the water content to 60% that nutrition accessory is adjusted in the back;
(2) wooden chopsticks are handled: wooden chopsticks are fined away, put into container, press with weight, with 1.0% sugar, 0.2-0.5% potassium primary phosphate and 0.1-0.3% sal epsom, make nutritive medium, wooden chopsticks are soaked 12-24h, or with nutritive medium poach 20-60min, allow the abundant moisture absorption of wooden chopsticks, do not have the dried heart and can pull out;
(3) pack is handled: roll in the nutrition accessory for preparing in the two wooden chopsticks ratios of every bag of 50-100, make wooden chopsticks fully touch nutrition accessory on every side up and down, will interspace between the wooden chopsticks, pack did not highly have wooden chopsticks 2cm, tying then;
(4) sterilization: autoclaving requires 1.5kg/cm
2Under the pressure, sterilized 2-2.5 hour; Normal-pressure sterilization was sterilized 10-12 hour under 100 ℃ of conditions of temperature;
(5) inoculation culture: sack is opened female kind of access mushroom particle, and inoculum size is 15%-20%, and temperature is controlled at 23 ± 1 ℃ in the culturing room of immigration cleaning then; Guarantee ventilation, mycelia can be covered with pocket in 25 days-30 days, and champignon chopstick strain is made;
(6) with the champignon chopstick strain cultivar of transferring:
Cultivar after the sterilization is directly put on the culturing rack,, reduced to 15-25 ℃, whole pair of chopstick strain directly inserted in the material, stay 1cm to seal, cultivated 25-35 days, make the cultivating champignon kind when the cultivating bag temperature at culturing room's disinfection together.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101588487A CN101456769A (en) | 2007-12-12 | 2007-12-12 | Bag-cultivation shiitake chopstick strain raw material formula and preparation process |
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| CNA2007101588487A CN101456769A (en) | 2007-12-12 | 2007-12-12 | Bag-cultivation shiitake chopstick strain raw material formula and preparation process |
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| CN101456769A true CN101456769A (en) | 2009-06-17 |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498942A (en) * | 2011-11-04 | 2012-06-20 | 顾环环 | Method for producing oyster mushroom strains from birch chopsticks |
| CN102763559A (en) * | 2011-05-05 | 2012-11-07 | 杨光 | Method for producing strains by recycling disposable chopsticks |
| CN103145482A (en) * | 2013-03-28 | 2013-06-12 | 岳阳云景生物科技有限公司 | Lentinus edodes culture medium mainly prepared from bamboo crumb and method for producing lentinus edodes by using culture medium |
| CN103214280A (en) * | 2013-04-05 | 2013-07-24 | 邬金飞 | Compatibility of 'strip' cultispecies compost of edible mushroom and manufacturing method thereof |
| CN103396257A (en) * | 2013-08-17 | 2013-11-20 | 邢台奥美菌业有限公司 | Culture material for bag-cultivated mushroom and preparation method thereof |
| CN103707376A (en) * | 2013-12-12 | 2014-04-09 | 重庆市福鑫洋食用菌有限公司 | Method for rapidly culturing edible fungus stock by using wood pole |
| CN104221718A (en) * | 2014-09-30 | 2014-12-24 | 汤阴县食用菌研究所 | Formula of edible fungi branch strain compost and preparation method of strain compost |
| CN104472223A (en) * | 2014-12-26 | 2015-04-01 | 青岛华盛绿能农业科技有限公司 | Method for fruiting bag-cultivated mushroom at one time |
| CN105237139A (en) * | 2015-08-29 | 2016-01-13 | 安远县天华现代农业有限责任公司 | Preparation and culturing method of tree branch bacterial strain of pleurotus eryngii and hypsizygus marmoreus |
| CN107593274A (en) * | 2017-10-31 | 2018-01-19 | 贵州省印江自治县梵净山生态菌业有限公司 | A kind of breeding method of mushroom |
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2007
- 2007-12-12 CN CNA2007101588487A patent/CN101456769A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102763559B (en) * | 2011-05-05 | 2015-08-26 | 杨光 | Utilize and reclaim the method that throwaway chopsticks make bacterial classification |
| CN102763559A (en) * | 2011-05-05 | 2012-11-07 | 杨光 | Method for producing strains by recycling disposable chopsticks |
| CN102498942A (en) * | 2011-11-04 | 2012-06-20 | 顾环环 | Method for producing oyster mushroom strains from birch chopsticks |
| CN103145482A (en) * | 2013-03-28 | 2013-06-12 | 岳阳云景生物科技有限公司 | Lentinus edodes culture medium mainly prepared from bamboo crumb and method for producing lentinus edodes by using culture medium |
| CN103214280A (en) * | 2013-04-05 | 2013-07-24 | 邬金飞 | Compatibility of 'strip' cultispecies compost of edible mushroom and manufacturing method thereof |
| CN103396257B (en) * | 2013-08-17 | 2015-08-19 | 邢台奥美菌业有限公司 | Bag-cultivation of shiitake fungus culture material and preparation method thereof |
| CN103396257A (en) * | 2013-08-17 | 2013-11-20 | 邢台奥美菌业有限公司 | Culture material for bag-cultivated mushroom and preparation method thereof |
| CN103707376A (en) * | 2013-12-12 | 2014-04-09 | 重庆市福鑫洋食用菌有限公司 | Method for rapidly culturing edible fungus stock by using wood pole |
| CN103707376B (en) * | 2013-12-12 | 2016-06-08 | 重庆市福鑫洋食用菌有限公司 | The method of rod fast culture edible fungi original seed |
| CN104221718A (en) * | 2014-09-30 | 2014-12-24 | 汤阴县食用菌研究所 | Formula of edible fungi branch strain compost and preparation method of strain compost |
| CN104221718B (en) * | 2014-09-30 | 2016-11-09 | 汤阴县食用菌研究所 | The formula of a kind of edible mushroom tree fungus compost and the preparation method of this compost |
| CN104472223A (en) * | 2014-12-26 | 2015-04-01 | 青岛华盛绿能农业科技有限公司 | Method for fruiting bag-cultivated mushroom at one time |
| CN105237139A (en) * | 2015-08-29 | 2016-01-13 | 安远县天华现代农业有限责任公司 | Preparation and culturing method of tree branch bacterial strain of pleurotus eryngii and hypsizygus marmoreus |
| CN107593274A (en) * | 2017-10-31 | 2018-01-19 | 贵州省印江自治县梵净山生态菌业有限公司 | A kind of breeding method of mushroom |
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Effective date of registration: 20090508 Address after: One hundred and ten thousand one hundred and sixty-one No. 84 Dongling Road, Dongling District, Liaoning, Shenyang, Postal Code: 110161 Applicant after: Liaoning Institute for Edible Fungus,Liaoning Academy of Agricultural Sciences Address before: Liaoning Province, Shenyang Dongling District No. 91 Guan Qiao Applicant before: Liaoning Dongfang Agricultural Science and Technology Co., Ltd. |
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