CN103214280A - Compatibility of 'strip' cultispecies compost of edible mushroom and manufacturing method thereof - Google Patents
Compatibility of 'strip' cultispecies compost of edible mushroom and manufacturing method thereof Download PDFInfo
- Publication number
- CN103214280A CN103214280A CN2013101714305A CN201310171430A CN103214280A CN 103214280 A CN103214280 A CN 103214280A CN 2013101714305 A CN2013101714305 A CN 2013101714305A CN 201310171430 A CN201310171430 A CN 201310171430A CN 103214280 A CN103214280 A CN 103214280A
- Authority
- CN
- China
- Prior art keywords
- strip
- stem stalk
- auxiliary material
- culture
- bag
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Landscapes
- Mushroom Cultivation (AREA)
Abstract
The invention relates to a 'strip' cultispecies compost of an edible mushroom. The compost is characterized in that the cultispecies of the edible mushroom is manufactured based on stalks of plants as raw materials. The invention further comprises a manufacturing method of the compost. Compared with the prior art, the invention has the advantages that 1, hypha in the strip is not damaged during grafting, the culture can germinate in a short time, and the whole strip germinates at the same to effectively inhibit growth of infectious microbe; 2, the anti-aging degree of hypha in the strip is high and the culture quality is improved; and 3, the cultispecies compost is short in age and consistent, so that mushroom is produced quickly and orderly, the production period is shortened, and the output and quality of products are improved.
Description
Technical field
Content of the present invention comprises edible mushrooms " strip " cultivar culture material compatibility, and the making method of this culture material, belong to edible fungus species and make the field,
Background technology
At present, China's edible mushroom cultivated species is the wood chip bacterial classification mostly.Because wood chip bacterial classification operation when inoculation is loaded down with trivial details, operating speed is slow, therefore easily be bacterial contamination, simultaneously, because bacterial classification often is shelved on the pre-opened hole of culture bottle (bag), can not enter the bottom of cultivation material, therefore it is also very slow to send out bacterium speed, often like this causes the aging and mycelia of bottom of top mycelia just just to begin the situation of growing, and promptly the cell age of upper and lower part mycelia is widely different, and this can cause fruiting irregular, influences output.
Make edible mushrooms " strip " cultivar, use the strip bacterial classification inoculation, can overcome the above problems.Allly contain xylogen, cellulosic plant haulm all can be used as production of hybrid seeds raw material.
One of raw material, ramie, Tong boon.Ramie, Tong fiber crops are the traditional fibre crops of China, and cultivated area is throughout the year more than 200,000 hectares, its tankage ramie bone, Tong boon rich cellulose, hemicellulose, its content of cellulose and the similar deciduous species of Mierocrystalline cellulose form.Therefore unusual suitable for making " strip " bacterial classification of these discarded ramies, Tong boon.
Two of raw material, reed stalk.The northeast of China, North China, the Inner Mongol, Xinjiang are the establishing in large scale zones of reed.The plant of reed is tall and big, straight and upright, its stem stalk xylogen, content of cellulose height, suitable for making " strip " bacterial classification.
Three of raw material, agricultural crop straws such as Chinese sorghum, grain, sesame, Sunflower Receptacle, cotton, soybean, corn.These agricultural crop straws distribute extensively, quantity is many, and its quality is sturdy and hard, and as production of hybrid seeds raw material, the potentiality of utilization are very big.
Summary of the invention
The present invention has following two aspect contents:
1, a kind of is edible mushrooms " strip " the cultivar culture material compatibility of main raw material with the agricultural crop straw.
2, the making method of edible mushrooms " strip " culture material.
The technical solution adopted in the present invention is:
A kind of edible mushroom cultivated species culture material has following component and quality proportioning: strip stem stalk 90%-95%, and auxiliary material 5%-10% is the quality of dry-matter, the proportioning sum is 100%;
Strip stem stalk: utilize agricultural crop straws (vertical profile 2-4 sheets such as the reed rod that the stem stalk is thicker, cornstalk) such as ramie bone, Tong boon, reed stalk and Chinese sorghum, grain, sesame, Sunflower Receptacle, cotton, soybean, corn, be cut into (0.5-1) cm
2* (12-16) cm size (determining kind of a bar length according to the height of the length of cultivating bag or bottle) is dried standby;
The component of auxiliary material and quality proportioning: hardwood sawdust 78%, wheat bran 20%, gypsum 1%, sugar 1%; In the making processes of cultivar, auxiliary material is filled in the gap of strip stem stalk.
The making method of a kind of edible mushrooms " strip " culture material comprises the steps:
1. strip stem stalk is handled: with the strip stem stalk Polywoven Bag of packing into, put into and be furnished with 1% sucrose, 0.3%KH
2PO
4Solution in soak 2h-6h, with weight suppression, to allow the stem stalk fully sink in the solution, to suction nutritive medium, whether do not have dried heart degree of being, after pulling out, fully drain away the water if suctioning to break the stem stalk into two with one's hands, puddle with a little dried auxiliary material then on it;
2. auxiliary material preparation: according to a conventional method each auxiliary material component is mixed thoroughly, mix Hou Jiashui thoroughly, last, the water content of compound is controlled at 55%-57%;
3. pack: adopt the high-pressure polyethylene bag tube of folding length 17cm, length 35cm, thick 0.05mm, spread one deck auxiliary material earlier, the vertical one deck strip stem stalk, all vacant parts of filling up cultivating bag then with auxiliary material, and compacting put in back.On uppermost top layer, add a cover the thick auxiliary material of 1cm.This processing can make the stem stalk can both fully touch nutrition from auxiliary material up and down.
4. sterilize, cool off: pocket is carried out autoclaving, make 100 ℃ of sterilization 1h earlier, make 121 ℃ of autoclaving 2h then; The type of cooling is: naturally cool to below 25 ℃;
After pocket inserted original seed, culturing room's temperature was controlled at 20 ℃-22 ℃, and relative air humidity is controlled at 60%-75%, and through the cultivation of 30d-32d, mycelia can be covered with whole bag.And then the after-ripening cultivation of process 2d-7d (according to stem stalk quality hardness decision time length), so that mycelial growth to the inside of stem stalk, promptly can be used for producing.
Use in the production: when inoculation culture bottle or bag, " strip " bacterial classification that covers with mycelia is directly inserted in the pre-opened hole of culture bottle (bag), every bottle (bag) inserts a root fungus bar, and auxiliary material is loose in charge level.After the inoculation, thermophilic is cultivated, and through the cultivation of 15d-20d, mycelia just can be covered with bottle (bag), sends out bacterium time ratio wood chip bacterial classification and shortens 10d-15d.Edible mushroomss such as Pleurotus eryngii, Pleurotus geesteranus, flat mushroom, Pleurotus sajor-caju, Coprinus comatus, Stropharia rugoso-annulata, straw mushroom, needle mushroom, YUHUANG MUSHROOM, dictyophora phalloidea are suitable for making " strip " cultivar all.
The present invention is a main raw material with the stem stalk of plant, makes edible mushroom cultivated species.Its advantage is: the one, and the mycelia in when inoculation bacterium bar is injury-free, and bacterial classification can be sprouted in a short time, and whole root fungus bar sends out bacterium simultaneously, has effectively suppressed the growth of assorted bacterium; The 2nd, the anti-aging degree height of mycelia has improved strain quality in the bacterium bar; The 3rd, cultivation bacterium material cell age is short and consistent, can make fruiting fast and neat like this, has shortened the production cycle, has improved the output and the quality of product.
Embodiment
Mode 1: contain abalone mushroom " strip " cultivar culture material compatibility in the present embodiment: ramie bone 92%, auxiliary material 8% all is the quality of dry-matter, the proportioning sum is 100%.
Strip stem stalk: the ramie bone is cut into (0.5-1) cm
2* 15cm size;
The component of auxiliary material and quality proportioning: hardwood sawdust 78%, wheat bran 20%, gypsum 1%, sugar 1%;
Making method:
1. strip stem stalk is handled: with the dry straw Polywoven Bag of packing into, put into and be furnished with 1% sucrose, 0.3%KH
2PO
4Solution in soak 5h, with weight suppression, to allow the stem stalk fully sink in the solution, to suction nutritive medium, whether do not have dried heart degree of being, after pulling out, fully drain away the water if suctioning to break the stem stalk into two with one's hands, puddle with a little dried auxiliary material then on it;
2. auxiliary material preparation: according to a conventional method each auxiliary material component is mixed thoroughly, mix Hou Jiashui thoroughly, last, the water content of compound is controlled at 55%;
3. pack: adopt the high-pressure polyethylene bag tube of folding length 17cm, length 35cm, thick 0.05mm, spread one deck auxiliary material earlier, the vertical one deck strip stem stalk, all vacant parts of filling up cultivating bag then with auxiliary material, and compacting put in back.On uppermost top layer, add a cover the thick auxiliary material of 1cm.This processing can make the stem stalk can both fully touch nutrition from auxiliary material up and down.
4. sterilize, cool off: pocket is carried out autoclaving, make 100 ℃ of sterilization 1h earlier, make 121 ℃ of autoclaving 2h then; The type of cooling is: naturally cool to below 25 ℃;
5. inoculation: after inserting original seed, culturing room's temperature is controlled at 20 ℃, and relative air humidity is controlled at 65%, and through the cultivation of 30d, mycelia is covered with whole bag.And then the after-ripening cultivation of process 5d, promptly can be used for the cultivating bag inoculation.
Utilize " strip " cultivar of present embodiment, during inoculation 1100ml * 78mmPP culture bottle, " strip " bacterial classification that covers with mycelia is directly inserted in the pre-opened hole of culture bottle, every bottle graft is gone into a root fungus bar, and auxiliary material is placed on charge level.After the inoculation, thermophilic is cultivated, and through the cultivation of 15d, mycelia is covered with whole bottle, sends out bacterium time ratio wood chip bacterial classification and shortens 10d.
Mode 2: Pleurotus geesteranus in the present embodiment " strip " cultivar culture material compatibility: cotton stalk 90%, auxiliary material 10% all is the quality of dry-matter, the proportioning sum is 100%.
Strip stem stalk: the cotton stalk is cut into (0.5-1) cm
2* 16cm size;
The component of auxiliary material and quality proportioning: hardwood sawdust 78%, wheat bran 20%, gypsum 1%, sugar 1%;
Making method reference example 1.Utilize the strip cultivar of present embodiment, during inoculation 17cm * 33cm cultivating bag, " strip " bacterial classification that covers with mycelia is vertically inserted in the pre-opened hole of cultivating bag, every bag is inserted a root fungus bar, and auxiliary material is placed on charge level.After the inoculation, thermophilic is cultivated, and through the cultivation of 20d, mycelia is covered with whole bag, sends out bacterium time ratio wood chip bacterial classification and shortens 12d.
Claims (8)
1. an edible mushrooms " strip " cultivar culture material is characterized in that containing following component and quality proportioning: strip stem stalk 90%-95%, and auxiliary material 5%-10% is the quality of dry-matter, the proportioning sum is 100%.
2. " strip " according to claim 1 culture material is characterized in that: it is raw material that strip stem stalk adopts ramie, Tong boon.
3. " strip " according to claim 1 culture material is characterized in that: it is raw material that strip stem stalk adopts reed stalk.
4. " strip " according to claim 1 culture material is characterized in that: it is raw material that strip stem stalk adopts agricultural crop straws such as Chinese sorghum, grain, sesame, Sunflower Receptacle, cotton, soybean, corn.
5. " strip " according to claim 1 culture material is characterized in that: strip stem stalk is cut into (0.5-1) cm
2* (12-16) cm size (determining kind of a bar length) according to the height of the length of cultivating bag or bottle.
6. culture material according to claim 1 is characterized in that: the component of auxiliary material and quality proportioning: hardwood sawdust 78%, wheat bran 20%, gypsum 1%, sugar 1%.
7. the making method of the culture material described in the claim 1~6 is characterized in that:
1. strip stem stalk is handled: with the strip stem stalk Polywoven Bag of packing into, put into and be furnished with 1% sucrose, 0.3%KH
2PO
4Solution in soak 2h-6h, with weight suppression, to allow the stem stalk fully sink in the solution, to suction nutritive medium, whether do not have dried heart degree of being, after pulling out, fully drain away the water if suctioning to break the stem stalk into two with one's hands, puddle with a little dried auxiliary material then on it;
2. auxiliary material preparation: according to a conventional method each auxiliary material component is mixed thoroughly, mix Hou Jiashui thoroughly, last, the water content of compound is controlled at 55%-57%;
3. pack: adopt the high-pressure polyethylene bag tube of folding length 17cm, length 35cm, thick 0.05mm, spread one deck auxiliary material earlier, the vertical one deck strip stem stalk of putting in back, fill up all vacant parts of cultivating bag then with auxiliary material, and compacting, on uppermost top layer, add a cover the thick auxiliary material of 1cm;
4. sterilize, cool off: pocket is carried out autoclaving, make 100 ℃ of sterilization 1h earlier, make 121 ℃ of autoclaving 2h then; The type of cooling is: naturally cool to below 25 ℃.
8. make cultivar with edible mushrooms " strip " culture material described in the claim 1-7, after it is characterized in that inserting original seed, culturing room's temperature is controlled at 20 ℃-22 ℃, relative air humidity is controlled at 60%-75%, through the cultivation of 30d-32d, mycelia can be covered with whole bag, and then cultivates through the after-ripening of 2d-7d (according to stem stalk quality decision time length), so that mycelial growth to the inside of stem stalk, promptly can be used for producing.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2013101714305A CN103214280A (en) | 2013-04-05 | 2013-04-05 | Compatibility of 'strip' cultispecies compost of edible mushroom and manufacturing method thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2013101714305A CN103214280A (en) | 2013-04-05 | 2013-04-05 | Compatibility of 'strip' cultispecies compost of edible mushroom and manufacturing method thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
CN103214280A true CN103214280A (en) | 2013-07-24 |
Family
ID=48812503
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2013101714305A Pending CN103214280A (en) | 2013-04-05 | 2013-04-05 | Compatibility of 'strip' cultispecies compost of edible mushroom and manufacturing method thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103214280A (en) |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103392507A (en) * | 2013-08-10 | 2013-11-20 | 黄艳芳 | Method for preparing bar-shaped strain |
CN103430769A (en) * | 2013-08-10 | 2013-12-11 | 黄艳芳 | Method for cultivating mushroom by strip-shaped corn straws |
CN103601596A (en) * | 2013-12-04 | 2014-02-26 | 邬金梅 | Method for preparing russula cultivation material by utilizing sunflower by-products |
CN103626553A (en) * | 2013-12-03 | 2014-03-12 | 邬金梅 | Method utilizing sunflower by-products to manufacture white fungus cultivation material |
CN103641556A (en) * | 2013-12-08 | 2014-03-19 | 邬金梅 | Method for manufacturing pholiota nameko culture material from sunflower byproducts |
CN103875458A (en) * | 2014-04-16 | 2014-06-25 | 山西奥格姆农业科技有限公司 | Method for shortening spawn running cycle of bagged cultivated pleurotus eryngii |
CN103951489A (en) * | 2014-04-19 | 2014-07-30 | 朱汉学 | Flammulina velutipes culture medium prepared from multiple weeds |
CN103951488A (en) * | 2014-04-19 | 2014-07-30 | 朱汉学 | Flammulina velutipes culture medium prepared from sunflower straws |
CN103951487A (en) * | 2014-04-19 | 2014-07-30 | 朱汉学 | Flammulina velutipes culture medium prepared from sesame/soybean straws |
CN104016772A (en) * | 2014-05-07 | 2014-09-03 | 合肥福泉现代农业科技有限公司 | Shiitake culture medium prepared from reed straw and preparation method thereof |
CN104221718A (en) * | 2014-09-30 | 2014-12-24 | 汤阴县食用菌研究所 | Formula of edible fungi branch strain compost and preparation method of strain compost |
CN110447461A (en) * | 2019-09-24 | 2019-11-15 | 贵州山环菌草科技有限公司 | A kind of cultural method improving edible mushroom yield and quality |
CN114027094A (en) * | 2021-10-29 | 2022-02-11 | 河北华绿之珍生物技术有限公司 | Reed straw composite culture medium and application thereof in industrialized cultivation of flammulina velutipes |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08294326A (en) * | 1995-04-28 | 1996-11-12 | Kansai Sogo Kankyo Center:Kk | Cultivation of mushroom |
JP4116362B2 (en) * | 2002-08-14 | 2008-07-09 | グアメイ マッシュルームス インコーポレイテッド | Method and apparatus for sterilization of plant planting base |
CN101456769A (en) * | 2007-12-12 | 2009-06-17 | 辽宁东方农业科技有限公司 | Bag cultivation champignon chopstick strain raw material formula and production process |
CN102422776A (en) * | 2011-09-08 | 2012-04-25 | 镇江市食用菌研究所 | Solid strain culture medium of edible fungi and production method of solid strain |
-
2013
- 2013-04-05 CN CN2013101714305A patent/CN103214280A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08294326A (en) * | 1995-04-28 | 1996-11-12 | Kansai Sogo Kankyo Center:Kk | Cultivation of mushroom |
JP4116362B2 (en) * | 2002-08-14 | 2008-07-09 | グアメイ マッシュルームス インコーポレイテッド | Method and apparatus for sterilization of plant planting base |
CN101456769A (en) * | 2007-12-12 | 2009-06-17 | 辽宁东方农业科技有限公司 | Bag cultivation champignon chopstick strain raw material formula and production process |
CN102422776A (en) * | 2011-09-08 | 2012-04-25 | 镇江市食用菌研究所 | Solid strain culture medium of edible fungi and production method of solid strain |
Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103392507A (en) * | 2013-08-10 | 2013-11-20 | 黄艳芳 | Method for preparing bar-shaped strain |
CN103430769A (en) * | 2013-08-10 | 2013-12-11 | 黄艳芳 | Method for cultivating mushroom by strip-shaped corn straws |
CN103626553A (en) * | 2013-12-03 | 2014-03-12 | 邬金梅 | Method utilizing sunflower by-products to manufacture white fungus cultivation material |
CN103601596A (en) * | 2013-12-04 | 2014-02-26 | 邬金梅 | Method for preparing russula cultivation material by utilizing sunflower by-products |
CN103641556A (en) * | 2013-12-08 | 2014-03-19 | 邬金梅 | Method for manufacturing pholiota nameko culture material from sunflower byproducts |
CN103641556B (en) * | 2013-12-08 | 2015-03-25 | 邬金梅 | Method for manufacturing pholiota nameko culture material from sunflower byproducts |
CN103875458A (en) * | 2014-04-16 | 2014-06-25 | 山西奥格姆农业科技有限公司 | Method for shortening spawn running cycle of bagged cultivated pleurotus eryngii |
CN103951487A (en) * | 2014-04-19 | 2014-07-30 | 朱汉学 | Flammulina velutipes culture medium prepared from sesame/soybean straws |
CN103951488A (en) * | 2014-04-19 | 2014-07-30 | 朱汉学 | Flammulina velutipes culture medium prepared from sunflower straws |
CN103951489A (en) * | 2014-04-19 | 2014-07-30 | 朱汉学 | Flammulina velutipes culture medium prepared from multiple weeds |
CN104016772A (en) * | 2014-05-07 | 2014-09-03 | 合肥福泉现代农业科技有限公司 | Shiitake culture medium prepared from reed straw and preparation method thereof |
CN104016772B (en) * | 2014-05-07 | 2016-02-10 | 合肥福泉现代农业科技有限公司 | A kind of reed stalk is mushroom culture medium of raw material and preparation method thereof |
CN104221718A (en) * | 2014-09-30 | 2014-12-24 | 汤阴县食用菌研究所 | Formula of edible fungi branch strain compost and preparation method of strain compost |
CN104221718B (en) * | 2014-09-30 | 2016-11-09 | 汤阴县食用菌研究所 | The formula of a kind of edible mushroom tree fungus compost and the preparation method of this compost |
CN110447461A (en) * | 2019-09-24 | 2019-11-15 | 贵州山环菌草科技有限公司 | A kind of cultural method improving edible mushroom yield and quality |
CN114027094A (en) * | 2021-10-29 | 2022-02-11 | 河北华绿之珍生物技术有限公司 | Reed straw composite culture medium and application thereof in industrialized cultivation of flammulina velutipes |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103214280A (en) | Compatibility of 'strip' cultispecies compost of edible mushroom and manufacturing method thereof | |
CN102037849B (en) | Tremella fuciformis strain culture method | |
CN103392501B (en) | A kind of ramulus mori elegant precious mushroom of cultivation and the method for regulation and control fruiting time | |
CN103224426B (en) | Novel method for preparing black fungus mushroom branched wood cultivars | |
CN104541987A (en) | Method for cultivating oyster mushrooms by fermented materials of corncobs | |
CN103210788A (en) | Industrialized production method of pleurotus eryngii | |
CN109197381B (en) | Method for planting hypsizygus marmoreus by liquefying solid strains | |
JP2011078394A (en) | Fermentation medium for edible mushroom and production method therefor | |
CN103371053A (en) | Method for cultivating green natural oyster mushrooms by using pure-length rice straws | |
CN102173883A (en) | New compost formula for industrial cultivation of pleurotus eryngii | |
CN102498937A (en) | Method for culturing oyster mushroom | |
CN101857488A (en) | Formula of cultivation material for cultivating pleurotus nebrodensis | |
CN103190290B (en) | A kind of method utilizing renewable resources formula to produce woodear | |
CN112021068A (en) | Rejuvenation method of tremella aurantialba sporocarp strains | |
CN107409833A (en) | A kind of preparation method of degradable planting container | |
CN104285677B (en) | A kind of preparation method of edible mushroom peg wood bacterial classification | |
CN103299822A (en) | Schizophyllum commune Fr high-yield fruiting body strain and three-dimensional ecology-returning cultivation method | |
CN109757299B (en) | Cultivation method for improving quality of ganoderma lucidum spore powder | |
US20100139157A1 (en) | Fungal bed cultivation method of hon-shimeji mushroom | |
CN103396218A (en) | Formula of Pleurotus citrinopileatus stock compost and preparation method of compost | |
KR101944613B1 (en) | Medium composite of shiitake and cultivation method using thereof | |
CN104686209A (en) | Method for cultivating mushroom by fermented biogas residue materials | |
KR20200012367A (en) | How to grow flower mushrooms | |
JPH02156828A (en) | Artificial cultivation of shiitake mushroom | |
CN104961562B (en) | A kind of mushroom cultivation material and the preparation method and application thereof of addition part sunflower seed shell |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C12 | Rejection of a patent application after its publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130724 |