CN103224426B - Novel method for preparing black fungus mushroom branched wood cultivars - Google Patents
Novel method for preparing black fungus mushroom branched wood cultivars Download PDFInfo
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- CN103224426B CN103224426B CN201310134683.5A CN201310134683A CN103224426B CN 103224426 B CN103224426 B CN 103224426B CN 201310134683 A CN201310134683 A CN 201310134683A CN 103224426 B CN103224426 B CN 103224426B
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Abstract
The invention relates to black fungus mushroom branched wood cultivars which are characterized by using branched wood as a main raw material and adding auxiliary materials as fillers. The invention also comprises a preparation method for the cultivars. Compared with conventional wood dust cultivars, the branched wood cultivars have the advantages that 1. mycelia of the branched wood are not damaged during inoculation, strains can germinate in a short period, and the whole branched wood germinates at the same time, so that growth of infectious microbes is inhibited; 2. the branched wood mycelia have high anti-aging degree, so that the quality of the strains is increased; 3. cultivated fungus sticks are short and consistent in fungus age, so that the black fungus and mushroom are produced quickly, and production period is shorten and yield and quality of the product are increased; and 4. inoculation speed is fast, operations are simple and fast, and practical applicability is strong.
Description
Technical field
The invention belongs to edible fungus species and make field, is a kind of method of preparing black fungus branch wood cultivar.
Background technology
At present, the cultivar of China's black fungus, is Saw-dust mostly.Because Saw-dust operation in the time inoculating is loaded down with trivial details, operating speed is slow, injured of bacterial classification is large, is therefore easily bacterial contamination, simultaneously, the degree of depth that enters culture material due to bacterial classification is inadequate, therefore send out bacterium speed also very slow, the normal like this top mycelia situation that mycelia aging and bottom has just just started growth that causes, the cell age of upper and lower part mycelia is widely different, and this can cause fruiting irregular, affect output.
The cultivar that adopts the wooden standby black fungus of branch, can overcome the above problems.Allly contain xylogen, cellulosic branch wood all can be used as production of hybrid seeds raw material.
One of raw material, solidago canadesis.Now, each region government is changing huge fund and is forcing down its growth radix, in this process, can produce a large amount of Herba Solidaginis canes.This cane is nontoxic, and after tested, before blooming, cane contains protein 0.58%, robust fibre 33.8%, and full nitrogen 0.92g/kg, full phosphorus 1.97g/kg, full potassium 10.7g/kg, wherein protein and crude fiber content approach the conventional culturing raw material wood chip of edible mushrooms and cotton seed hulls.Therefore, Herba Solidaginis cane can be used as a raw material for wood fungus kind, carries out recycling.
Two of raw material, the discarded branch in the woody medicinal material woodss such as the bark of official magnolia, the bark of eucommia, this class branch dense materials, light and soft, suitable for making branch wood fungus kind.
Three of raw material, the fruit such as mulberry tree, peach, Japanese apricot, chestnut, grape, citrus, willow, oak, maple, Chinese parasol tree, weeping willow with and greening abandon branch with after the cutting of trees, these abandon a material compared with consolidation, very suitable for making branch wood fungus kind.
Summary of the invention
The present invention has following two aspects:
1, a kind of cultivation of auricularia auricula material compatibility taking branch wood as main raw material.
2, the making method of black fungus branch wood cultivar.
The technical solution adopted in the present invention is:
A kind of cultivation of auricularia auricula material, has following component and quality proportioning: the wooden 85%-95% of branch, and auxiliary material 5%-15%, is the quality of dry-matter, proportioning sum is 100%;
Branch wood: the stem stalk that utilizes solidago canadesis, trunk, branch, the twigs of the chaste tree that medicinal material woods (bark of official magnolia, the bark of eucommia etc.), fruit tree (mulberry tree, peach, chestnut, Japanese apricot, grape, citrus etc.) and landscape tree (willow, oak, maple, Chinese parasol tree, weeping willow etc.) are pruned, except the residual leaf of impurity elimination branch, be cut into (0.5-1) cm
2× (8-10) cm (determining length according to the diameter of producing bacterium bag) size, one end wedgewise, dries for subsequent use;
The component of auxiliary material and quality proportioning: hardwood sawdust 78%, wheat bran 20%, gypsum 1%, sugar 1%; In branch wood planting material making processes, auxiliary material is filled in the gap of branch wood.
A making method for black fungus branch wood cultivar, comprises the steps:
1. branch wood is processed: will do branch wood and pack Polywoven Bag into, and put the sucrose, the 0.3%KH that are furnished with 1% into
2pO
4solution in soak 8h-10h, on it, with weight suppression, to allow a wood fully sink in solution, to suction nutritive medium, whether suction to break into two with one's hands branch wood without dry heart degree of being, after pulling out, fully drain away the water, then puddle with a little dry auxiliary material;
2. auxiliary material preparation: according to a conventional method each auxiliary material component is mixed thoroughly, add water after mixing thoroughly, last, the water content of compound is controlled at 55%-57%, auxiliary material preparation has accused;
3. pack: adopt the high-pressure polyethylene bag cylinder of folding length 17cm, length 35cm, thick 0.05mm, first spread one deck auxiliary material, after put one deck branch wood, and then put one deck auxiliary material, then put again one deck branch wood, placement so from level to level, until pile.Here, auxiliary material will fill up all vacant part of cultivating bag, and compacting.On uppermost top layer, add a cover the auxiliary material of 1cm.This processing can make branch wood can fully touch up and down the nutrition from auxiliary material.
4. sterilizing, cooling: pocket is carried out to autoclaving, first make 100 DEG C of sterilizing 1h, then make 121 DEG C of autoclaving 2h; The type of cooling is: naturally cool to below 25 DEG C;
5. inoculation: after access original seed, culturing room's temperature is controlled at 20 DEG C-22 DEG C, and relative air humidity is controlled at 60%-75%, through the cultivation of 30d-32d, mycelia can be covered with whole bag.Because branch wood is compared with wood chip close structure, therefore cover with after whole bag mycelia, must pass through again the After-mature cultivation of 7d-20d (hardwood material ripening time is long, and loose material is shorter), so that mycelial growth is to the xylem of branch wood.
Production application: when bacterial classification inoculation, one of the wedge shape of the branch wood that covers with mycelia is inserted in cultivating bag, on the cultivating bag that is generally 45cm in stock column length, inserted uniformly four (cultivating bag traverses), the 2mm of the wooden end of branch stays outside bag, to play the sealing process of inoculation position.After inoculation, thermophilic is cultivated, and through the cultivation of 35d-38d, mycelia just can be covered with whole bag, and a bacterium time shortens 12d-15d than Saw-dust.
The present invention, taking branch wood as main raw material, makes cultivation of auricularia auricula kind.Its advantage is: the one, and the mycelia in when inoculation branch wood is injury-free, and bacterial classification can be sprouted in a short time, and whole branch wood sends out bacterium simultaneously, has effectively suppressed the growth of miscellaneous bacteria; The 2nd, in a wood, the anti-aging degree of mycelia is high, has improved strain quality; The 3rd, the bacterium rod cell age of cultivation is short and consistent, can use like this ear (mushroom) soon and neatly, shortens the production cycle, improves output and the quality of product; The 4th, inoculation speed is fast, easy and simple to handle, quick, practical.
Embodiment
Mode 1: in the present embodiment, mushroom branch wood cultivar comprises following component and quality proportioning: branch wood 90%, auxiliary material 10%.
Branch wood: be the stem stalk of solidago canadesis, be cut into (0.5-1) cm
2× 8cm size, one end wedgewise;
The component of auxiliary material and quality proportioning: hardwood sawdust 78%, wheat bran 20%, gypsum 1%, sugar 1%;
Making method:
1. branch wood is processed: will do branch wood and pack Polywoven Bag into, and put the sucrose, the 0.3%KH that are furnished with 1% into
2pO
4solution in soak 8h, after pulling out, fully drain away the water, then puddle with a little dry auxiliary material;
2. auxiliary material preparation: spice according to a conventional method, the water content of auxiliary material is controlled at 55%-57%;
3. pack: the high-pressure polyethylene bag cylinder that adopts folding length 17cm, length 35cm, thick 0.05mm, first spread one deck auxiliary material, on it, put one deck branch wood, and then put one deck auxiliary material, then put one deck branch wood again, place so from level to level, auxiliary material will fill up the vacant part of sack, and compacting, the auxiliary material of 1cm is added a cover on surface;
4. sterilizing, cooling: pocket is carried out to autoclaving, first keep 100 DEG C of sterilizing 1h, then rise to 121 DEG C of autoclaving 2h; The type of cooling: naturally cool to below 25 DEG C;
5. inoculation: after access original seed, culturing room's temperature is controlled at 20 DEG C-22 DEG C, and relative air humidity is controlled at 60%-75%, through the cultivation of 30d, mycelia is covered with full bag, and then the After-mature cultivation of process 7d, can be used for cultivating bag inoculation.
Utilize the wooden cultivar of branch of the present embodiment, the shiitake cultivation bag that inoculation stock column length is 45cm, through the cultivation of 35d, mycelia just can be covered with full bag, and a bacterium time shortens 15d than Saw-dust.
Mode 2: in the present embodiment, black fungus branch wood cultivar comprises following component and quality proportioning: branch wood 85%, auxiliary material 15%.
Branch wood: by peach branch, be cut into (0.5-1) cm
2× 8cm size, one end wedgewise;
The component of auxiliary material and quality proportioning: hardwood sawdust 78%, wheat bran 20%, gypsum 1%, sugar 1%;
Making method reference example 1, because the material of peach is compared with consolidation, therefore After-mature cultivation time lengthening is 15d.
Utilize the wooden cultivar of branch of the present embodiment, the cultivation of auricularia auricula bag that inoculation stock column length is 45cm, through the cultivation of 38d, mycelia just can be covered with full bag, and a bacterium time shortens 12d than Saw-dust.
Claims (1)
1. a black fungus branch wood cultivar, is characterized in that, culture material contains following component and quality proportioning: the wooden 85%-95% of branch, and auxiliary material 5%-15%, is the quality of dry-matter, proportioning sum is 100%; Described branch wood adopts peach branch; Described auxiliary material component and quality proportioning are: hardwood sawdust 78%, wheat bran 20%, gypsum 1%, sugar 1%; The making method of described black fungus branch wood cultivar is:
1. branch wood is processed: will do branch wood and be cut into (0.5-1) cm
2× (8-10) cm size, one end wedgewise, pack Polywoven Bag into, put into be furnished with 1% sucrose, the solution of 0.3%KH2PO4 soaks 8h-10h, on it, suppress with weight, to allow a wood fully sink in solution, to suction nutritive medium, after pulling out, fully drain away the water, then puddle with a little dry auxiliary material;
2. auxiliary material preparation: according to a conventional method each auxiliary material component is mixed thoroughly, add water after mixing thoroughly, last, the water content of compound is controlled at 55%-57%;
3. pack: adopt the high-pressure polyethylene bag cylinder of folding length 17cm, length 35cm, thick 0.05mm, first spread one deck auxiliary material, after put one deck branch wood, and then put one deck auxiliary material, then put again one deck branch wood, placement so from level to level, until pile;
4. sterilizing, cooling: Bag Material is carried out to autoclaving, first make 100 DEG C of sterilizing 1h, then make 121 DEG C of autoclaving 2h; The type of cooling is: naturally cool to below 25 DEG C;
5. inoculation: after access original seed, culturing room's temperature is controlled at 20 DEG C-22 DEG C, and relative air humidity is controlled at 60%-75%, through the cultivation of 30d-32d, mycelia can be covered with whole bag; Because branch wood is compared with wood chip close structure, therefore cover with after whole bag mycelia, must pass through again the After-mature cultivation of 7d-20d, so that mycelial growth is to an xylem for branch wood.
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| CN103387466B (en) * | 2013-08-02 | 2014-12-24 | 邬金飞 | Black fungus culture compatible product and preparation method of culture |
| CN103704011A (en) * | 2013-12-09 | 2014-04-09 | 广西柳城县成霖农业科技有限公司 | High-yield fungus package for cultivation of black funguses |
| CN104106377B (en) * | 2014-07-31 | 2015-12-02 | 桂林丰茂源农业技术开发有限公司 | The cultivation method of woodear |
| CN104311256B (en) * | 2014-09-29 | 2017-10-03 | 云南省农业科学院经济作物研究所 | A kind of mushroom cultivation substrate made of industrial hemp |
| CN104221718B (en) * | 2014-09-30 | 2016-11-09 | 汤阴县食用菌研究所 | The formula of a kind of edible mushroom tree fungus compost and the preparation method of this compost |
| CN104478546B (en) * | 2014-11-03 | 2016-09-21 | 河北大学 | A kind of method utilizing Vitex chinensis Mill. bits to cultivate hedgehog hydnum |
| CN104429591B (en) * | 2014-11-03 | 2016-07-06 | 河北大学 | A kind of method utilizing Vitex chinensis Mill. bits to cultivate Auricularia polytricha (Mout) Sacc. |
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| CN104744140A (en) * | 2015-03-04 | 2015-07-01 | 邬金梅 | Preparation method of black fungus cultivation material |
| CN104744139A (en) * | 2015-03-04 | 2015-07-01 | 邬金梅 | Preparation method of black fungus cultivation material |
| CN104973958A (en) * | 2015-06-25 | 2015-10-14 | 广西柳城县成霖农业科技有限公司 | Black fungus culture substrate and preparation method therefor |
| CN105859415A (en) * | 2016-04-14 | 2016-08-17 | 上海大学 | Novel auricularia auricula culture material and preparation method |
| CN106538242A (en) * | 2016-11-01 | 2017-03-29 | 陈天泰 | A kind of willow section edible mushroom cultivated species compost and cultigen preparation method |
| CN109997603A (en) * | 2019-03-19 | 2019-07-12 | 南京康之春生物科技有限公司 | A kind of cultural method of the artificial Bag Material ganoderma lucidum of optimization |
| CN111466253A (en) * | 2020-03-27 | 2020-07-31 | 安康市农业科学研究院 | Cultivation method and application of mushroom cultivar |
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| DE4012671C2 (en) * | 1990-04-20 | 1994-05-19 | Grabbe Klaus | Use of liquid manure prepared by rotting |
| CN1235755A (en) * | 1999-05-14 | 1999-11-24 | 安晨明 | Technology for producing dendritical spawn of bag cultivated edible mushroom |
| CN1806496A (en) * | 2006-01-24 | 2006-07-26 | 陈若霞 | Edible fungus cultivation method |
| CN102523934B (en) * | 2012-02-10 | 2013-06-12 | 国家林业局泡桐研究开发中心 | Production method for cultivating functional eucommia wood ear |
| CN102603403A (en) * | 2012-03-23 | 2012-07-25 | 福建农林大学 | Mushroom cultivation substrate |
| CN102835247B (en) * | 2012-08-09 | 2014-07-16 | 颍上县鸿涛菌业专业合作社 | Method for cultivating agaric with mulberry branches |
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