CN102173883A - New compost formula for industrial cultivation of pleurotus eryngii - Google Patents
New compost formula for industrial cultivation of pleurotus eryngii Download PDFInfo
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- CN102173883A CN102173883A CN2011100374129A CN201110037412A CN102173883A CN 102173883 A CN102173883 A CN 102173883A CN 2011100374129 A CN2011100374129 A CN 2011100374129A CN 201110037412 A CN201110037412 A CN 201110037412A CN 102173883 A CN102173883 A CN 102173883A
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Abstract
The invention relates to a new compost formula for the industrial cultivation of pleurotus eryngii, which comprises 46% of wood dust, 14% of cottonseed husk, 18% of chaff, 18% of corn cob and 4% of corn meal. The new compost formula for the industrial cultivation of pleurotus eryngii has the advantages of appropriate carbon-nitrogen ratio, favorable physical behavior of culture media, fast growth of hyphae, short cultivation period and high first flush yield. By using the wood dust as the main raw material, the new compost formula is low in cost, thereby being applicable to the requirement of the industrial cultivation for only harvesting the first flush of pleurotus eryngii.
Description
Technical field
The invention belongs to the preparation prescription of edible fungus species, particularly a kind of culture material of Pleurotus eryngii industrial cultivation is newly filled a prescription.
Background technology
Pleurotus eryngii (Pleurotus eryngii (DC exFr.) Que1.) belongs to the Tricholomataceae pleurotus, and the another name eryngo is picked up the ears.Pleurotus eryngii is not only delicious in taste, nutritious, and has very high pharmaceutical use.Traditional cultivation technique can only be chosen in the time of the year when autumn changes into winter according to weather condition and cultivate, and can't satisfy people's consumer need throughout the year.
Summary of the invention
The invention provides with a kind of culture material of Pleurotus eryngii industrial cultivation and newly fill a prescription.It is to be the culture material prescription with wood chip, cotton seed hulls, wheat bran, corn cob, Semen Maydis powder, fine particle calcium carbonate, unslaked lime, by cultivating the fruiting experiment, and mycelial growth and microbiological contamination situation, sporophore appearance character, fruiting body yield, biological transformation ratio studied, finally determined Pleurotus eryngii industrial cultivation economical character culture material prescription preferably.
Concrete scheme of the present invention
The new prescription of a kind of culture material of Pleurotus eryngii industrial cultivation is: wood chip 46%, cotton seed hull 14%, wheat bran 18%, corn cob 18%, Semen Maydis powder 4%.
Characteristics of the present invention
It is suitable that the new prescription of the culture material of this Pleurotus eryngii industrial cultivation has carbon-nitrogen ratio, and the substratum physical behavior is good, and mycelial growth rate is fast, it is short to send out the bacterium time, damp mushroom output height is that the major ingredient cost is low with the wood chip, is applicable to only the gather requirement of a damp mushroom of factory culture.
Embodiment
Experiment material and method:
The Pleurotus eryngii bacterial classification is contained agrotechnique development corporation, Ltd. by letter shore, Tianjin standing grain and is provided.Culturing raw material: wood chip, cotton seed hulls, wheat bran, corn cob, Semen Maydis powder, fine particle calcium carbonate, unslaked lime.
The formulating of recipe of culture material
According to the practical situation of factory culture, and carried out Formula Design (table 2) with reference to the method for orthogonal experiment, each level of factor sees Table 1.Not designing in strict accordance with percentage composition at horizontal design aspect, also is the practical situation of considering the mass-producing operation, and the raw materials quality that adds with reality is used as level relatively, has good applicability.
Table 1 empirical factor level
Table 2 orthogonal experiment conceptual design (kg)
According to the different culture material of table 2 preparation, each prescription adds the fine particle calcium carbonate of gross weight 1% and 2% unslaked lime again.The fresh nothings of ingredient requirement such as Semen Maydis powder, cotton seed hulls, corn cob, wheat bran are gone mouldy.Earlier water is added in the raw material before the spice, make raw material fully absorb moisture after, be put in the special-purpose stirring machine respectively and stir.The culture material water content is controlled at 65%, regulates the pH value between 7.2-7.5.
2.3 the pack of culture material
Culture material is contained in the polypropylene plastics pocket of 17cm * 36cm, an opening with sack packer, charging requires evenly, degree of tightness is moderate, the sack collar, and with the sack turnover, cotton puts dampproof cover beyond the Great Wall.Pack quantity sees Table 3.
Table 3 culture material pack quantity
2.4 culture material sterilization, inoculation and cultivation
The bacterium bag is carried out high pressure steam sterilization, 121 ℃ of temperature, time 2.5h.Put into the cooling room cooling after the sterilization, when temperature is reduced to below 35 ℃, inoculate.In inoculation tank, adopt aseptic technique, tree fungus is directly inserted in the bacterium bag.Culturing room's constant temperature, dark culturing, temperature 23-25 ℃.Beginning is checked once weekly after cultivating 3-5d, in time sorts out and has polluted or dysgonic bacterium bag.Mycelia is covered with immigration fruiting room, back and carries out the after-ripening processing.
2.5 management of producing mushroom and gathering
Enter the management of producing mushroom stage after the after-ripening.The measures such as bag, mycelium stimulation of opening are carried out according to a conventional method.After forming original hase, begin to draw mouth, improve oxygen supply.The mushroom flower bud is long during to the Semen arachidis hypogaeae size, dredges with pocket knife and goes deformity and the overstocked mushroom flower bud of part.The mushroom lid is basic to launch, and does not gather when spore launches, and the damp mushroom of gathering is pruned laggard line data collection on request.
The bright product output (g) of biology efficient=sporophore/culture material dry mass (g) * 100%
2.6 Data Processing in Experiment
2.6.1 mycelial growth and microbiological contamination situation
Culture bag in each prescription is carried out the observation on Growth of mycelia, the results are shown in Table 4.As shown in Table 4, No. 2, No. 3, No. 5, No. 7, No. 8, No. 9 prescription mycelia growing way is better; Mycelia purseful fate is stabilized in about 25 days basically, and No. 6, No. 1 the prescription mycelial growth is slower, and bigger from the carbon-nitrogen ratio of these two prescriptions of analysis of filling a prescription, it is poor to nourish and grow, and mycelial growth is unable, will produce adverse influence to the growth of the sporophore in later stage; Aspect microbiological contamination, No. 9 pollution condition is comparatively serious, though the Pleurotus eryngii anti-hybrid ability is poor, the bacterium bag pollution rate of all prescriptions is all less than surpassing 7%, illustrate that disinfectant measure and environment are controlled can satisfy the factory culture requirement.
The Pleurotus eryngii mycelia all can grow in 9 culture material prescriptions, but the culture material prescription mycelial growth with 2,3,5, No. 8 is the most vigorous, and pollution rate is lower simultaneously, may be that mycelial growth is rapid, can obtain growth vigor at short notice, thereby suppress the partly growth of assorted bacterium.No. 1 and No. 4 poor growths, pollution rate is higher.
Table 4 different material prescription mycelial growth status investigation
2.6.2 the comparison of sporophore appearance character
Cap mean diameter 2.00cm as can be seen from Table 5 fill a prescription for No. 4, stem mean diameter 4.19cm, No. 6 prescription is respectively 1.79cm and 4.90cm, No. 8 prescription is respectively 2.75cm and 4.80cm, the cone shape of " big down little " appears in sporophore that these several prescriptions are described mostly, does not meet the commodity requirement of Pleurotus eryngii.The parton entity of No. 7 and No. 9 occurs spherical, can't measure cap, stem diameter, no commodity value.The bacteria cover diameter and the stem diameter of No. 2, No. 3, No. 5 and No. 9 are more or less the same, and most sporophores present bar-shaped, and the mushroom type is good, meets the standard of commodity mushroom.
The investigation of table 5 different material prescription sporophore stem and cap
As can be seen from Table 6, the sporophore abnormal rate of 6,7,8, No. 9 culture material prescriptions is higher, has reached more than 70%, may be because the decomposition of culture material nutrient and accumulation can not be satisfied the requirement that sporophore growth is grown, though can produce sporophore, sporophore tends to deformity, output is also low.2, the sporophore of 3, No. 5 culture material prescriptions presents bar-shapedly, and abnormal rate is controlled at below 14%, and cultivating rate is up to more than 94%, and the cycle is shorter, and the prescription of comparing other has remarkable advantages in the decomposition of nutrient aspect utilizing.
The investigation of table 6 different material prescription sporophore proterties
2.6.3 fruiting body yield relatively
Come as can be seen from Table 7, No. 2 prescription sporophore weight in average maximums reach 0.38kg, have competitive edge preferably aspect mode of appearance.3,5 numbers it, be respectively 0.34kg and 0.33kg, 4, No. 7 the poorest.Between single mushroom fresh weight and bacteria cover diameter, the stem length substantial connection is arranged, along with the single mushroom fresh weight of the increase of bacteria cover diameter, stem length also can correspondingly increase, but in Pleurotus eryngii industrial production, can artificially suppress its cap size for the commodity value that improves Pleurotus eryngii, make its bacteria cover diameter substantially with stem diameter unanimity, with the form of control commodity mushroom, thereby improve its exterior quality.
Table 7 different material prescription single bag of output of sporophore (kg)
2.6.4 the different ingredients biological transformation ratio is analyzed
As can be seen from Table 8, No. 2 biological transformation ratio has reached 91.4%, No. 3 also to 76.5%, and the biological transformation ratio less than 30% of No. 4 and No. 7.Method according to orthogonal experiment is carried out range analysis (result is unlisted) to each processing, carries out randomized blocks analysis of variance simultaneously, the results are shown in Table 9.
Table 8 different material prescription sporophore biological transformation ratio statistics (%)
The variance analysis of table 9 different material prescription sporophore biological transformation ratio
F test shows that biological transformation ratio difference is not remarkable between district's group, illustrates that (5 sampling) preparation of raw material and other experiment condition difference are little between 5 district's groups, and experimental data is measured credible.C factor (Semen Maydis powder) differences is not remarkable, explanation is under 3 set concentration, its biological transformation ratio does not have significant difference, the inequality heteropole is remarkable between A factor (cotton seed hulls), B factor (wheat bran), D factor (corn cob) differences is remarkable, illustrates that all there were significant differences for its biological transformation ratio under 3 set concentration, need further make multiple comparisons, the results are shown in Table 10, table 11, table 12.
Biological transformation ratio significance of difference test between table 10 different content cotton seed hulls
Biological transformation ratio significance of difference test between table 11 different content wheat bran
Biological transformation ratio significance of difference test between table 12 different content corn cob
By table 10, the explanation of table 11 analytical results, there is significant difference between the cotton seed hulls different content, the highest with A1 (7.5kg) biological transformation ratio, its utmost point is significantly higher than A2 (15kg) and A3 (22.5kg); Also have significant difference between the wheat bran different content, higher with B2 (10kg) and B3 (15kg) biological transformation ratio, they are apparently higher than B1 (5kg); And there is not utmost point conspicuous level between the corn cob different content.According to the situation of experimental variance statistics, the sporophore biological transformation ratio difference between each prescription is (data are unlisted) extremely significantly, and in order further to determine the significance of difference between group, the multiple comparisons between treatment combination the results are shown in Table 13.
The table 13 different ingredients Pleurotus eryngii biological transformation ratio significance of difference
As can be seen from Table 13, the difference of biological transformation ratio is not remarkable between No. 2 and No. 3 prescription, and No. 2 prescription is compared significant difference or extremely remarkable with other prescriptions, No. 2 prescription can be used as optimum combination of the present invention, and promptly wood chip 46%, cotton seed hull 14%, wheat bran 18%, corn cob 18%, Semen Maydis powder 4%.
Claims (1)
1. the culture material of a Pleurotus eryngii industrial cultivation is newly filled a prescription, and it is characterized by: wood chip 46%, cotton seed hull 14%, wheat bran 18%, corn cob 18%, Semen Maydis powder 4%.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102523925A (en) * | 2012-01-09 | 2012-07-04 | 福建省农业科学院土壤肥料研究所 | Bag-cultured edible fungus industrialized culture method capable of preventing cotton plug in culture bag from being wetted |
| CN102550288A (en) * | 2012-01-09 | 2012-07-11 | 福建省农业科学院土壤肥料研究所 | Edible fungus strain preparation method capable of preventing damp cotton plugs in strain bottles or culture bags |
| CN103254000A (en) * | 2013-06-05 | 2013-08-21 | 山东福禾菌业科技有限公司 | Pleurotus eryngii compost formula |
| CN104140310A (en) * | 2014-08-21 | 2014-11-12 | 湖南省宇秀生物科技有限公司 | Culture medium for industrial cultivation of pleurotus eryngii |
| CN104909927A (en) * | 2015-06-19 | 2015-09-16 | 桂林健成生物科技开发有限公司 | Application of seed coats/embryos and rhizomes after sprouting vegetable collection in pleurotus eryngii culture |
| CN105110995A (en) * | 2015-09-29 | 2015-12-02 | 福建省农业科学院食用菌研究所 | Pleurotus eryngii culture medium containing pig farming padding and preparation method thereof |
| CN108147875A (en) * | 2018-03-07 | 2018-06-12 | 常熟理工学院 | A kind of factory culture pleurotus eryngii cultivating material |
| CN113854039A (en) * | 2021-10-20 | 2021-12-31 | 贵州贵旺生物科技有限公司 | Industrial production method of pleurotus eryngii |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101684054A (en) * | 2009-03-27 | 2010-03-31 | 广东星河生物科技股份有限公司 | Formula of culture medium for high yield beech mushrooms and production process |
| CN101935257A (en) * | 2010-09-28 | 2011-01-05 | 上海市农业科学院 | Culture material for pleurotus eryngii |
-
2011
- 2011-02-14 CN CN2011100374129A patent/CN102173883A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101684054A (en) * | 2009-03-27 | 2010-03-31 | 广东星河生物科技股份有限公司 | Formula of culture medium for high yield beech mushrooms and production process |
| CN101935257A (en) * | 2010-09-28 | 2011-01-05 | 上海市农业科学院 | Culture material for pleurotus eryngii |
Non-Patent Citations (1)
| Title |
|---|
| 廖志敏等: "杏鲍菇工厂化栽培配方的研究", 《第二届全国食用菌中青年专家学术交流会论文集》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102523925A (en) * | 2012-01-09 | 2012-07-04 | 福建省农业科学院土壤肥料研究所 | Bag-cultured edible fungus industrialized culture method capable of preventing cotton plug in culture bag from being wetted |
| CN102550288A (en) * | 2012-01-09 | 2012-07-11 | 福建省农业科学院土壤肥料研究所 | Edible fungus strain preparation method capable of preventing damp cotton plugs in strain bottles or culture bags |
| CN103254000A (en) * | 2013-06-05 | 2013-08-21 | 山东福禾菌业科技有限公司 | Pleurotus eryngii compost formula |
| CN104140310A (en) * | 2014-08-21 | 2014-11-12 | 湖南省宇秀生物科技有限公司 | Culture medium for industrial cultivation of pleurotus eryngii |
| CN104140310B (en) * | 2014-08-21 | 2016-08-24 | 湖南省宇秀生物科技有限公司 | A kind of culture medium of factory culture Pleurotus eryngii |
| CN104909927A (en) * | 2015-06-19 | 2015-09-16 | 桂林健成生物科技开发有限公司 | Application of seed coats/embryos and rhizomes after sprouting vegetable collection in pleurotus eryngii culture |
| CN105110995A (en) * | 2015-09-29 | 2015-12-02 | 福建省农业科学院食用菌研究所 | Pleurotus eryngii culture medium containing pig farming padding and preparation method thereof |
| CN105110995B (en) * | 2015-09-29 | 2018-06-15 | 福建省农业科学院食用菌研究所 | A kind of pleurotus eryngii cultivating material of the bedding and padding containing pig raising and preparation method thereof |
| CN108147875A (en) * | 2018-03-07 | 2018-06-12 | 常熟理工学院 | A kind of factory culture pleurotus eryngii cultivating material |
| CN113854039A (en) * | 2021-10-20 | 2021-12-31 | 贵州贵旺生物科技有限公司 | Industrial production method of pleurotus eryngii |
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Application publication date: 20110907 |