CN102422776A - Solid strain culture medium of edible fungi and production method of solid strain - Google Patents
Solid strain culture medium of edible fungi and production method of solid strain Download PDFInfo
- Publication number
- CN102422776A CN102422776A CN2011102648621A CN201110264862A CN102422776A CN 102422776 A CN102422776 A CN 102422776A CN 2011102648621 A CN2011102648621 A CN 2011102648621A CN 201110264862 A CN201110264862 A CN 201110264862A CN 102422776 A CN102422776 A CN 102422776A
- Authority
- CN
- China
- Prior art keywords
- solid spawn
- bar
- production method
- culture matrix
- solid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Mushroom Cultivation (AREA)
Abstract
The invention provides a solid strain culture medium of edible fungi and a production method of the solid strain. The solid strain culture medium is characterized in that the solid strain culture medium is a strip object obtained by taking used disposable chopsticks as raw materials, washing the raw materials to remove stains, cutting the raw materials in classification, and dewatering and drying the cut raw materials. The production method of the solid strain comprises the steps of soaking, washing, draining, mixing with supplementary materials, loading, sterilizing, inoculating, culturing and the like. In the invention, the used disposable chopsticks are used as the raw materials, so that the resource utilization of solid wastes is realized, the wastes are changed into useful things, and the disadvantage is turned into the advantage; the environment pollution can be reduced, and the production cost of the strain can be greatly reduced; the economic benefit for fungi farmers to cultivate the edible fungi is increased, thus, the effect that shooting two birds with one stone can be get; and moreover, the development direction of low carbon economy and cycle economy is met, thus, the culture medium and the production method can be promoted and applied throughout the country.
Description
Technical field
The present invention relates to a kind of edible fungus culture medium, particularly relate to the production method of a kind of edible mushroom solid spawn culture matrix and solid spawn, belong to the Edible Fungi field.
Background technology
Seed is a lifespring, as the saying goes " spread what seed and open what flower ", " good kind has gone out seedling, and good seedling output is high ".This shows that seed is the basic factor of decision crop yield and quality, Edible Fungi also is that so bacterial classification is the necessary condition of Edible Fungi.Traditional edible fungus species is divided into two types of liquid spawn and solid spawns; Chinese patent CN 101717309A discloses medium of the rotten edible mushroom solid spawn of a kind of grass and the preparation method of solid spawn; The component of this medium and weight portion ratio thereof are peat soil 68～78, cotton seed hulls 10～15, wheat bran 10～15; Gypsum 1～2, sucrose 1～2 and the pH value that adds lime adjustment medium are 6.5～7.0.Chinese patent CN 1179622C discloses a kind of Agaricus blazei Murrill cultivated species culture medium prescription and preparation method, and its concrete prescription is by weight percentage: wheat berry 85～90%, broad-leaved wood chip 9～12%, sucrose 0.2～2%, gypsum 0.2～1%, urea 0.05～0.4%.Chinese patent CN 101941868A discloses a kind of culture medium of edible fungus, comprises the raw material of following parts by weight: wood chip 75-79, wheat skin 17-23, expect smart 2-4.Said material spermatophore is drawn together the raw material of following parts by weight: calcium carbonate 26-30, calcium sulphate 26-30, magnesium sulfate 8-9, potassium dihydrogen phosphate 8-9, lime 8-12, the urea 8-9 that contains 46% nitrogen, triacontanol 8-9, peptone 3-5, beef extract 2-3.In the prior art, the culture matrix of solid spawn mainly contains cotton seed hulls, corncob, wood chip or wheat, grain, branch etc.At present, the most frequently used bacterial classification raw materials for production cotton seed hulls, price per ton increases to about 2000 for 1000 yuan from less than in 2008, and provision prices such as wheat are higher.
Summary of the invention
The objective of the invention is to go up, cause the deficiency of bacterial classification production cost increase, a kind of edible mushroom solid spawn culture matrix of novel cheapness and the production method of solid spawn thereof are provided to conventional solid bacterium culture medium cost of material.
Technical scheme of the present invention realizes in the following manner:
A kind of edible mushroom solid spawn culture matrix is characterized in that: said solid spawn culture matrix is to be raw material with used throwaway chopsticks, through cleaning the bar after decontamination, classification are cut off, dehydrated.
Said throwaway chopsticks are wooden or bamboo material is processed, and nothing is gone mouldy, the round chopsticks of diameter 3-5mm or width 5-7mm, the flat chopsticks of thickness 3-5mm; Length is 160-240mm.
Said cleaning decontamination is that the hot water that the throwaway chopsticks of collecting are put into earlier more than 80 ℃ is removed greasy dirt, rinses the drop branch that anhydrates again well with running water.
It is that the throwaway chopsticks that clean up are classified by different length earlier that said classification is cut off, and again it evenly is cut into the 2-3 section, forms the bar of length 5-8mm.
Said dehydrating is to adopt the method dry or dry that the water content of bar is dropped to below 14%.
A kind of method of utilizing above-mentioned solid spawn culture matrix to produce the edible mushroom solid spawn is characterized in that carrying out as follows:
(1) soak: with the bar dry product that throwaway chopsticks are processed to form, the limewash with 1% soaked 5-7 hour, made it fully suction moisture, did not have the white heart;
(2) clean: will pass through bar that limewash soaks and put into clear water and clean, and make the pH value reduce to 7-8;
(3) drain: put into the basket drop branch that anhydrates through the bar that cleans, dry to water content 55-60% subsequent use;
(4) join auxiliary material: the dried bar of every kg is joined the dried auxiliary material of 0.1-0.2kg, and the adjunct ingredient weight ratio is wood chip or corncob 60-75%, wheat bran 15-25%, corn flour 5-10%, land plaster 1%, sugar 1%, auxiliary material water content 60-65%;
(5) charging: the bar that will dry is earlier uprightly packed in vial or the special plastic bag, and again at the surface coverage auxiliary material, auxiliary material thickness 0.3-0.6cm seals bottleneck or sack;
(6) sterilization: 1.5-2kg/cm
2The autoclaving of pressure kept 3-4 hour, and normal-pressure sterilization kept 12-18 hour;
(7) inoculation: in gnotobasis, insert edible fungus species, inoculum concentration 10-20%;
(8) cultivate: will inoculate good container and put into culturing room's cultivation 25-35 days of 20-28 ℃.
Beneficial effect of the present invention:
1, the present invention is a raw material with used throwaway chopsticks, realizes the recycling of solid wastes utilization, meets low-carbon economy, recycling economy development direction.
2, be the culture matrix of edible mushroom solid spawn with the solid waste, turn waste into wealth, turn harm into good, not only can reduce environmental pollution, and can reduce the bacterial classification production cost greatly, improve the economic benefit of mushroom farming culturing edible fungus, kill two birds with one stone.
Embodiment
Below in conjunction with specific embodiment; Further illustrate the present invention; Should understand this embodiment only be used to the present invention is described and be not used in the restriction scope of the present invention; After having read the present invention, those skilled in the art all fall within the application's accompanying claims institute restricted portion to the modification of the various equivalent form of values of the present invention.
Embodiment 1:
A kind of edible mushroom solid spawn culture matrix is to be raw material with used throwaway chopsticks, through cleaning the bar after decontamination, classification are cut off, dehydrated.
Said throwaway chopsticks are that wood materials is processed, and nothing is gone mouldy, the round chopsticks of diameter 3mm or width 5mm, the flat chopsticks of thickness 3mm; Length is 210mm.
The hot water of at first throwaway chopsticks of collecting being put into earlier more than 80 ℃ cleans, and, rinses the drop branch that anhydrates again well to remove greasy dirt with running water.
Then the throwaway chopsticks that clean up are classified by different length earlier, again it evenly is cut into 3 sections, form the bar of length 7mm.
Adopt the method for drying to carry out dried at last, the water content of bar dry product is dropped to below 14%.
A kind of method of utilizing above-mentioned solid spawn culture matrix to produce the edible mushroom solid spawn is characterized in that carrying out as follows:
(1) soak: with the bar dry product that throwaway chopsticks are processed to form, the limewash with 1% soaked 5 hours, made it fully suction moisture, did not have the white heart;
(2) clean: will pass through bar that limewash soaks and put into clear water and clean, and make the pH value reduce to 7;
(3) drain: put into the basket drop branch that anhydrates through the bar that cleans, dry to water content 58% subsequent use;
(4) join auxiliary material: the dried bar of every kg is joined the dried auxiliary material of 0.15kg, and the adjunct ingredient weight ratio is wood chip or corncob 63%, wheat bran 25%, corn flour 10%, land plaster 1%, sugar 1%, auxiliary material water content 63%;
(5) charging: the bar that will dry is earlier uprightly packed in vial or the special plastic bag, and again at the surface coverage auxiliary material, auxiliary material thickness 0.3cm seals bottleneck or sack;
(6) sterilization: 1.5-2kg/cm
2The autoclaving of pressure kept 3 hours, and normal-pressure sterilization kept 12 hours;
(7) inoculation: in gnotobasis, insert edible fungus species, inoculum concentration 10%;
(8) cultivate: will inoculate good container and put into culturing room's cultivation 25 days of 20-28 ℃.
Embodiment 2:
Said throwaway chopsticks are that bamboo material is processed, and nothing is gone mouldy, the round chopsticks of diameter 4mm or width 6mm, the flat chopsticks of thickness 4mm; Length is 160mm.
The hot water of earlier throwaway chopsticks of collecting being put into earlier about 90 ℃ cleans, and, rinses the drop branch that anhydrates again well to remove greasy dirt with running water.
Then the throwaway chopsticks that clean up are classified by different length, again it evenly is cut into 2 sections, form the bar of length 8mm.
Adopt the method for oven dry that bar is carried out dried at last, its water content is dropped to below 14%.
A kind of method of utilizing above-mentioned solid spawn culture matrix to produce the edible mushroom solid spawn is characterized in that carrying out as follows:
(1) soak: with the bar dry product that throwaway chopsticks are processed to form, the limewash with 1% soaked 6 hours, made it fully suction moisture, did not have the white heart;
(2) clean: will pass through bar that limewash soaks and put into clear water and clean, and make the pH value reduce to 7.5;
(3) drain: put into the basket drop branch that anhydrates through the bar that cleans, dry to water content 60% subsequent use;
(4) join auxiliary material: the dried bar of every kg is joined the dried auxiliary material of 0.2kg, and the adjunct ingredient weight ratio is wood chip or corncob 75%, wheat bran 18%, corn flour 5%, land plaster 1%, sugar 1%, auxiliary material water content 60-65%;
(5) charging: the bar that will dry is earlier uprightly packed in vial or the special plastic bag, and again at the surface coverage auxiliary material, auxiliary material thickness 0.5cm seals bottleneck or sack;
(6) sterilization: 1.5-2kg/cm
2The autoclaving of pressure kept 3.5 hours, and normal-pressure sterilization kept 18 hours;
(7) inoculation: in gnotobasis, insert edible fungus species, inoculum concentration 20%;
(8) cultivate: will inoculate good container and put into culturing room's cultivation 30 days of 20-28 ℃.
Claims (6)
1. the production method of edible mushroom solid spawn culture matrix and solid spawn is characterized in that: said solid spawn culture matrix is to be raw material with used throwaway chopsticks, through cleaning the bar after decontamination, classification are cut off, dehydrated.
2. the production method of a kind of edible mushroom solid spawn culture matrix according to claim 1 and solid spawn; It is characterized in that: said throwaway chopsticks are wooden or bamboo material is processed; Nothing is gone mouldy, the round chopsticks of diameter 3-5mm or width 5-7mm, the flat chopsticks of thickness 3-5mm; Length is 160-240mm.
3. the production method of a kind of edible mushroom solid spawn culture matrix according to claim 1 and solid spawn; It is characterized in that: said cleaning decontamination is that the hot water that the throwaway chopsticks of collecting are put into earlier more than 80 ℃ is removed greasy dirt; Rinse the drop branch that anhydrates again well with running water.
4. the production method of a kind of edible mushroom solid spawn culture matrix according to claim 1 and solid spawn; It is characterized in that: it is that the throwaway chopsticks that clean up are classified by different length earlier that said classification is cut off; Again it evenly is cut into the 2-3 section, forms the bar of length 5-8mm.
5. the production method of a kind of edible mushroom solid spawn culture matrix according to claim 1 and solid spawn is characterized in that: said dehydrating is to adopt the method dry or dry that the water content of bar is dropped to below 14%.
6. method of utilizing the described solid spawn culture matrix of claim 1 to produce the edible mushroom solid spawn is characterized in that carrying out as follows:
(1) soak: with the bar dry product that throwaway chopsticks are processed to form, the limewash with 1% soaked 5-7 hour, made it fully suction moisture, did not have the white heart;
(2) clean: will pass through bar that limewash soaks and put into clear water and clean, and make the pH value reduce to 7-8;
(3) drain: put into the basket drop branch that anhydrates through the bar that cleans, dry to water content 55-60% subsequent use;
(4) join auxiliary material: the dried bar of every kg is joined the dried auxiliary material of 0.1-0.2kg, and the adjunct ingredient weight ratio is wood chip or corncob 60-75%, wheat bran 15-25%, corn flour 5-10%, land plaster 1%, sugar 1%, auxiliary material water content 60-65%;
(5) charging: the bar that will dry is earlier uprightly packed in vial or the special plastic bag, and again at the surface coverage auxiliary material, auxiliary material thickness 0.3-0.6cm seals bottleneck or sack;
(6) sterilization: 1.5-2kg/cm
2The autoclaving of pressure kept 3-4 hour, and normal-pressure sterilization kept 12-18 hour;
(7) inoculation: in gnotobasis, insert edible fungus species, inoculum concentration 10-20%;
(8) cultivate: will inoculate good container and put into culturing room's cultivation 25-35 days of 20-28 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102648621A CN102422776A (en) | 2011-09-08 | 2011-09-08 | Solid strain culture medium of edible fungi and production method of solid strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102648621A CN102422776A (en) | 2011-09-08 | 2011-09-08 | Solid strain culture medium of edible fungi and production method of solid strain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102422776A true CN102422776A (en) | 2012-04-25 |
Family
ID=45956878
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011102648621A Pending CN102422776A (en) | 2011-09-08 | 2011-09-08 | Solid strain culture medium of edible fungi and production method of solid strain |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102422776A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103214280A (en) * | 2013-04-05 | 2013-07-24 | 邬金飞 | Compatibility of 'strip' cultispecies compost of edible mushroom and manufacturing method thereof |
| CN103707376A (en) * | 2013-12-12 | 2014-04-09 | 重庆市福鑫洋食用菌有限公司 | Method for rapidly culturing edible fungus stock by using wood pole |
| CN104106370A (en) * | 2014-06-04 | 2014-10-22 | 保山富群农业科技有限公司 | Liquid strain of edible mushrooms and method for producing liquid strain of edible mushrooms |
| CN104370651A (en) * | 2014-12-03 | 2015-02-25 | 遵义市高谋食用菌种植场 | Medium for culturing edible fungi |
| CN104429586A (en) * | 2013-09-18 | 2015-03-25 | 孙君莲 | Edible fungus liquid strain multi-point rapid spawn running method in cultivation bag |
| CN105900692A (en) * | 2016-05-24 | 2016-08-31 | 黑龙江八农垦大学 | Method for cultivating hericium erinaceus by means of corncobs |
| CN108812082A (en) * | 2018-05-30 | 2018-11-16 | 江苏圣福来生态农业有限公司 | A kind of edible mushroom greenhouse cultivation method |
-
2011
- 2011-09-08 CN CN2011102648621A patent/CN102422776A/en active Pending
Non-Patent Citations (4)
| Title |
|---|
| 任桂梅 等: "废筷子制作食用菌枝条菌种初探", 《安徽农业科学》 * |
| 唐玉琴 等: "废弃方便筷子制食用菌栽培种技术", 《中国林副特产》 * |
| 张连合 等: "食用菌高效制种及栽培技术研究", 《北方园艺》 * |
| 李会杰 等: "利用枝条菌种高效栽培平菇", 《食用菌》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103214280A (en) * | 2013-04-05 | 2013-07-24 | 邬金飞 | Compatibility of 'strip' cultispecies compost of edible mushroom and manufacturing method thereof |
| CN104429586A (en) * | 2013-09-18 | 2015-03-25 | 孙君莲 | Edible fungus liquid strain multi-point rapid spawn running method in cultivation bag |
| CN103707376A (en) * | 2013-12-12 | 2014-04-09 | 重庆市福鑫洋食用菌有限公司 | Method for rapidly culturing edible fungus stock by using wood pole |
| CN103707376B (en) * | 2013-12-12 | 2016-06-08 | 重庆市福鑫洋食用菌有限公司 | The method of rod fast culture edible fungi original seed |
| CN104106370A (en) * | 2014-06-04 | 2014-10-22 | 保山富群农业科技有限公司 | Liquid strain of edible mushrooms and method for producing liquid strain of edible mushrooms |
| CN104106370B (en) * | 2014-06-04 | 2016-08-24 | 保山富群农业科技有限公司 | A kind of edible fungi liquid strain and production method thereof |
| CN104370651A (en) * | 2014-12-03 | 2015-02-25 | 遵义市高谋食用菌种植场 | Medium for culturing edible fungi |
| CN105900692A (en) * | 2016-05-24 | 2016-08-31 | 黑龙江八农垦大学 | Method for cultivating hericium erinaceus by means of corncobs |
| CN108812082A (en) * | 2018-05-30 | 2018-11-16 | 江苏圣福来生态农业有限公司 | A kind of edible mushroom greenhouse cultivation method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102422776A (en) | Solid strain culture medium of edible fungi and production method of solid strain | |
| CN101627699B (en) | Method for planting edible fungi and producing artificial grass peat by using cabo | |
| CN105176881B (en) | A method of producing the engineering microbial inoculum and its production active bio-organic fertilizer of active bio-organic fertilizer | |
| CN100551227C (en) | A kind of Volvaria volvacea cultivation waste material method of cultivating agaricus bisporus on the spot of utilizing | |
| CN105379560A (en) | Method for cultivating edible fungi | |
| CN106242669A (en) | A kind of biological organic fertilizer | |
| CN103880527B (en) | A kind of be main raw material with pure cassava wastes culture medium of edible fungus, Preparation Method And The Use | |
| CN101914450B (en) | Fungus agent for biologically dewatering compost and preparation method thereof | |
| CN102617208A (en) | Edible fungus residue organic fertilizer raw materials and preparation method thereof | |
| CN104621050A (en) | Food chain culturing method of maggots and earthworms | |
| CN103102189A (en) | Organic fertilizer formula making use of factory sludge and seafood mushroom dregs and production process of organic fertilizer formula | |
| CN103583227A (en) | Method for cultivating good-quality high-yield hericium erinaceus by means of mulberry branches | |
| CN103483064A (en) | Culture medium using waste pomegranate twigs as matrix and preparation method thereof | |
| AU2020102268A4 (en) | Edible fungus culture medium based on disposable bamboo chopsticks and preparation method and application thereof | |
| CN102379211A (en) | Method for planting king oyster mushroom by using grape branch | |
| CN103086767A (en) | High-potassium organic composite fertilizer special for dragon fruit and production method using domestic sludge | |
| CN101946608B (en) | Method for cultivating oyster mushroom by using banana stems | |
| CN106278752A (en) | A kind of mineral organic fertilizer | |
| CN107721630A (en) | Using White mushroom planting material of the snake room bedding and padding as primary raw material of giving up | |
| CN104560941A (en) | Method for enhancing worm grass fermentation biomass | |
| CN102503664B (en) | Edible fungus cultivation medium containing waste feed as major ingredient and preparation method thereof | |
| CN107602287A (en) | It is a kind of to utilize ferment bacterium compost of preserved apple processing byproduct and preparation method thereof | |
| CN103621310A (en) | Wild beef mushroom successfully trained and cultivated by using mountain grass and mushroom residues | |
| Khan et al. | Efficiency of oyster mushroom Pleurotus columbinus-P 8 using date palm leaves with combination of wheat straw and cotton waste for its yield improvement | |
| CN103373866A (en) | Preparation method of microbial organic fertilizer capable of preventing and treating root rot of crops |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120425 |